scholarly journals Comparative GC-MS analysis of the composition of metabolites of aboveground and underground parts of Comarum palustre L

2021 ◽  
Vol 10 (4) ◽  
pp. 95-103
Author(s):  
J. Strugar ◽  
A. A. Orlova ◽  
M. N. Povydysh
Keyword(s):  
Secondary Metabolites ◽  
Raw Materials ◽  
Biologically Active ◽  
Quantitative Composition ◽  
Leningrad Region ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Plant Raw Materials ◽  
Comarum Palustre ◽  
The Relationship

Introduction. Understanding the mechanisms of accumulation of individual groups of biologically active substances in promising types of plant raw materials and the possibility of predicting them is important for solving fundamental and applied problems of pharmaceuticals. To date, differences have been revealed in the qualitative and quantitative composition of secondary metabolites in the aboveground and underground of Comarum palustre L., however, the issue remains unstudied.Aim. Comparative metabolomic study of the composition of the primary metabolites of the aboveground and underground parts of Comarum palustre L.Materials and methods. The object of the study was the aboveground and underground parts of Comarum palustre L., harvested in the vicinity of the nursery of medicinal plants of the St. Petersburg State Chemical and Pharmaceutical University (Leningrad Region, Vsevolozhsky District, Priozerskoe Highway, 38 km) in 2019 and dried. Metabolomic studies based on GC-MS method was perfomed. A statistical analysis based on the MetaboAnalyst 5.0 platform was used.Results and discussion. Analysis of the chromatograms obtained using the GC-MS method revealed the content of 933 primary metabolites in the aboveground and underground parts of Comarum palustre L., 120 of which were identified. Using a number of statistical methods, 10 metabolites from monosaccharides, acids and alcohols, making the greatest contribution to the manifestation of differences between the studied samples, were identified.Conclusion. The study revealed the relationship between the composition of primary and secondary metabolites in medicinal plant raw materials.

Comparative Histochemical analysis of above-ground parts of Filipendula vulgaris and Filipendula ulmaria growing in Central Kazakhstan

2021 ◽  
pp. 4863-4867
Author(s):  
Yerbolat Tulebayev A. ◽  
Margarita Ishmuratova Yu. ◽  
Irina Losseva V. ◽  
Wirginia Kukuła-Koch ◽  
Ewa Poleszak ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Raw Materials ◽  
Sesquiterpene Lactones ◽  
Biologically Active ◽  
Histochemical Analysis ◽  
Filipendula Ulmaria ◽  
Plant Raw Materials ◽  
Related Substances ◽  
Histochemical Tests ◽  
The Republic

The histochemical analysis of medicinal plants and raw materials makes it possible to establish the presence of certain groups of active and related substances or products of their metabolism, as well as to study their localization in tissues. The article presents the results of histochemical analysis of leaves, flowers, petioles and stems of Filipendula vulgaris and Filipendula ulmaria. These types of plant are used in traditional medicine as an anti-inflammatory, antipyretic and bactericidal agent. Our research objective - to determine the peculiarities of the localization features of individual groups of secondary metabolites in the aerial part of meadowsweet and meadowsweet growing in the territory of Central Kazakhstan at the microscopic level. Materials and Methods. Transverse sections of petioles, stem and whole flower; transverse and superficial sections of leaves of Filipendula vulgaris and Filipendula ulmaria were fixed in Strauss-Fleming solution, after which histochemical reactions were carried out to locate and identify essential oils, phenolic acids, flavonoids, sesquiterpene lactones, polysaccharides and alkaloids. The study of microscopic signs, as well as histochemical tests were carried out according to the methods of the State Pharmacopoeia of the Republic of Kazakhstan using a light microscope Biomed-4. Results and Discussion. The localization of biologically active substances in the studied types of plant raw materials was determined by histochemical methods using light microscopy. The presence of phenolic compounds, flavonoids, traces of essential oil, alkaloids and sesquiterpene lactones was established. The absence of polysaccharides in the investigated micropreparations was established. Conclusions. For the first time, the localization of secondary metabolites in the tissues of Filipendula vulgaris and Filipendula ulmaria was studied by histochemical tests. The results of histochemical studies can be used to confirm the authenticity, identification and standardization of the aerial parts of Filipendula vulgaris and Filipendula ulmaria.

TECHNOLOGY OF PRODUCTION OF BIOLOGICALLY ACTIVE PLANT COMPLEX: DETERMINATION OF REGULATED PARAMETERS, FUNCTIONAL PROPERTIES

2019 ◽  
Keyword(s):  
Functional Properties ◽  
Viral Infections ◽  
Raw Materials ◽  
Extraction Process ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Composition ◽  
Comprehensive Treatment ◽  
Plant Raw Materials ◽  
Technology Of Production

The technology of production of a biologically active phytocomplex in the form of a biologically active additive has been discussed. The qualitative and quantitative composition of the formulation, which forms the functional properties of a specialized product, has scientifically been determined. The main stages of production include the preparation and input control of the raw material, the preparation of a mixture for encapsulation, including the steps of dispensing, sieving, grinding and mixing at a rate of 100 kg / 1 h, encapsulation and deducting, packaging, packaging and storage. A description of the technology of ex-traction of plant raw materials has been given, which is of great importance in the formation of consumer properties of the product being developed. The extraction process includes: obtaining liquid recovery us-ing demineralized water with dissolved in it sodium bicarbonate in an amount of 8% of the dry feed con-tent. The extractant-raw ratio is 1:13, the extraction time is 4 hours for each time; thickening of the liquid extract. It is carried out by means of vacuum in a vacuum evaporator plant before obtaining an extract with a solids content of not less than 20%; obtaining a dry extract. It is carried out by spray drying at a temperature of 90-95 ° C. The advantage of the developed technology of dietary supplements is the possi-bility of combining several differently directed biologically active substances in one capsule. The gelati-nous membrane reliably protects the contents from various environmental factors. Functional properties of the formulation formula of a specialized product are aimed at the prevention and comprehensive treatment of infectious viral diseases, confirmed by the conclusion of experts from the Russian Federal Service for Consumer Rights and Consumer Protection and the results of clinical trials in a group of pa-tients with acute respiratory viral infections. The developed product has been approved and produced at the enterprises of the company «Art Life».

scholarly journals Comparative Phytochemical Analyses and Metabolic Profiling of Different Phenotypes of Chinese Cabbage (Brassica Rapa ssp. Pekinensis)

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 587 ◽  
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Soo-Yun Park ◽  
Jae Kwang Kim ◽  
Sang Un Park
Keyword(s):  
Secondary Metabolites ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
High Performance ◽  
Total Carotenoids ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Phenolic Contents ◽  
Flight Mass Spectrometry ◽  
The Relationship

This study aimed to comprehensively examine the interface between primary and secondary metabolites in oval- and rectangular-shaped Chinese cabbage (Brassica rapa ssp. pekinensis) using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In addition to differences in shape, there was significant morphological variation between the two cultivars. The rectangular variety had greater height and deeper green color, whereas the oval variety had more leaves and greater width. A total of 42 primary metabolites identified by GC-TOFMS were subjected to partial least-squares discriminant, which indicated significant differences in the primary and secondary metabolisms of the two cultivars. Furthermore, total glucosinolate and phenolic contents were higher in the oval cultivar, whereas the rectangular cultivar contained a higher level of total carotenoids. This metabolome study comprehensively describes the relationship between primary and secondary metabolites in the oval and rectangular cultivars of Chinese cabbage and provides information useful for developing strategies to enhance the biosynthesis of glucosinolates, phenolics, and carotenoids in Chinese cabbage. Additionally, this work highlights that HPLC and GC-TOFMS–based metabolite profiling is suitable techniques to determine metabolic differences in Chinese cabbage.

scholarly journals ASSESSMENT OF THE CONTENT OF POLYPHENOLS IN BIOTECHNOLOGICAL RAW MATERIAL IRIS SIBIRICA L. STAR VARIETY IN COMPARISON WITH INTACT PLANTS

2019 ◽  
pp. 239-250 ◽  
Author(s):  
Ekaterina Alekseevna Antipova ◽  
Lyudmila Evdokimovna Kudrikova ◽  
Lyudmila Ivanovn Tikhomirova ◽  
Natalya Grigoryevna Bazarnova ◽  
Marina Yurievna Cheprasova ◽  
...  
Keyword(s):  
Raw Materials ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Raw Material ◽  
Soil Conditions ◽  
Quantitative Composition ◽  
Active Compounds ◽  
Plant Raw Materials ◽  
Intact Plants ◽  
Growing Conditions

Cultivation of plant raw materials in artificial conditions will inevitably lead to a change in the qualitative and quantitative composition of secondary metabolites in comparison with the composition of plants obtained in soil conditions. The study of the metabolic profile of such plants is very important on the one hand for our understanding of temporal (ontogenetic) and spatial changes in the metabolome of plants, on the other hand in the applied plan to obtain an alternative quality medicinal plant raw materials. In this regard, the aim of this study was to study the content of phenolic compounds in biotechnological raw materials Iris sibirica Grade Стерх in comparison with intact plants. As objects of study used plants propagated mikrokanale (regenerants), which were further grown under conditions of hydroponics (hydroponic). Intact plants were harvested in the field at the age of six years. As a result of research in raw materials Iris sibirica Grade Стерх, grown under different conditions, were found following a group of biologically active compound (БАС), corresponding to the genus Iris L.: fenilpropionovoy acid (Komarova and ferulic acid and their derivatives), flavonoids (glycosides of apigenin, apigenin-7-O-glycoside), isoflavones, and phenolic acids (vanillic acid), neytralizuya flavonoids-glycosides (glycosides of kaempferol and apigenin), stilbene. The qualitative composition of biologically active compounds of Iris sibirica Стерх depended on the growing conditions, while the biotechnological raw materials (hydroponic plants) are the closest to intact plants in terms of the content of biologically active compounds.  This allows us to consider the biotechnology of obtaining the raw material Iris sibirica L. based on hydroponic cultivation coupled with the micropropagation in an alternative way.

scholarly journals Methods for the determination of flavonoids in plant raw materials and products of their processing

2020 ◽  
Author(s):  
N.V. Alpatova ◽  
◽  
I.A. Dubrovskaya ◽  
M.V. Slobodyanik ◽  
◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Comparative Analysis ◽  
Literature Review ◽  
Raw Materials ◽  
Biologically Active ◽  
Quantitative Composition ◽  
Plant Materials ◽  
Qualitative And Quantitative ◽  
Plant Raw Materials

Flavonoids are an important component of plant materials; they have medicinal and biologically active properties. To use them as medicines, it is necessary to determine their qualitative and quantitative composition. The aim of this work is a comparative analysis of the identification and metrological characteristics of methods for the determination of flavonoids in plant raw materials and products of their processing. The paper presents a brief literature review of the main methods for the study of flavonoids. Certified and advanced techniques, such as NMR and mass spectrometry, have been discussed.

scholarly journals Widely Targeted Metabolomics Analysis of Different Parts of Salsola collina Pall

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1126
Author(s):  
Shipeng Li ◽  
Ye Chen ◽  
Ying Duan ◽  
Yinhui Zhao ◽  
Di Zhang ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Principal Component ◽  
Biologically Active ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Medicinal Value ◽  
History Of ◽  
Widely Targeted Metabolomics ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Active Substances

Salsola collina Pall has a long history of being used as a traditional medicine to treat hypertension, headache, insomnia, constipation and vertigo. However, only a few biologically active substances have been identified from S. collina. Here, the shoots and roots of S. collina, namely L-Sc and R-Sc, were studied. The primary and secondary metabolites were investigated using ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). A total of 637 putative metabolites were identified and these metabolites were mainly classified into ten different categories. Correlation analysis, hierarchical clustering analysis, principal component analysis and orthogonal partial least squares discriminant analysis of metabolites showed that the L-Sc samples could be clearly separated from the R-Sc samples. Differential accumulated metabolite analysis revealed that most of differential primary metabolites were significantly lower in the L-Sc than in the R-Sc. Conversely, the major differential secondary metabolites had higher levels in the L-Sc than in the R-Sc. Further analysis indicated that the flavonoids were the major putative antioxidant components and most of putative antioxidant components exhibited higher relative concentrations in the L-Sc than the R-Sc. These results improve our understanding of metabolite accumulation and provide a reference for the study of medicinal value in S. collina.

COMPARATIVE ANALYSIS OF BIOACTIVE SUBSTANCES CONTENT IN THE LEAVES AND FRUITS OF A ROWAN-TREE (SORBUS AUCUPARIA FRUCTUS) AND ASSESSMENT OF PROSPECTS IN MEDICINE AND PHARMACY

2020 ◽  
pp. 58-62
Author(s):  
Chernichkina A.D.
Keyword(s):  
Raw Materials ◽  
Wide Distribution ◽  
Biologically Active ◽  
Bioactive Substances ◽  
Plant Raw Materials ◽  
Mountain Ash ◽  
The Russian Federation ◽  
Materials Used ◽  
Active Substances ◽  
Pectin Substances

A large number of biologically active substances, organic acids, tannins, and pectin substances were detected during the study of fruits, leaves, and pulp of the fruits of mountain Ash. The content of these substances in leaves and pulp will significantly expand the arsenal of medicinal plant raw materials used. Given the wide distribution of mountain Ash in the territory of the Russian Federation, harvesting leaves from the same plants after harvesting the fruit and using pulp will make it possible to obtain new phytopreparations.

On the diagnostics of Adonis L. species (Ranunculaceae) of the Russian flora

2020 ◽  
pp. 3-14
Author(s):  
Aleksandr Luferov
Keyword(s):  
Raw Materials ◽  
Natural Populations ◽  
Structural Features ◽  
Biologically Active ◽  
Scientific Medicine ◽  
Diagnostic Features ◽  
Plant Raw Materials ◽  
Similar Chemical ◽  
Chemical Studies ◽  
Alternative Sources

The article provides brief information about cardiotonic, sedative, cytostatic, diuretic, and antibacterial effects of biologically active compounds of Adonis L. (Ranunculaceae) species. Chemical studies allowed to identify the cardiac glycosides, or cardenolides: or cardenolides: adontoxin, adonitol, adonitoxigenin, acetyldigitoxin and others. In scientific medicine, it is currently allowed to use Adonis vernalis L. Other types of Adonis have a similar chemical composition and are offered as substitutes for this official species, for example, Adonis apennina L. Many Adonis species have limited natural resources, and in some regions are rare, requiring conservation of their natural populations. The search for alternative sources of medicinal plant raw materials, based on this, is relevant. The experimental part of our research was carried out using the morphological and geographical method with the involvement of information on ecology and phenology. For the first time summarizes the diagnostic features of Adonis flora of Russian flora. Previously unknown structural features (shape and size of anthers) were identified that characterize the subgenera Adonanthe and Adonis. Taxonomic study of the genus Adonis of the Russian flora allowed us to determine its species composition, clarify its systematic affiliation, and nomenclature synonyms. 9 species were identified. Of these, 6 are perennials belonging to the subgenus Adonanthe, section Consiligo, which includes 2 subsections: Amurenses (2 species) and Vernales, which is differentiated into 2 rows: Apenninae (2 species) and Vernales (2 species). Subgenus Adonis is represented by 2 sections: Adonis (1 species) and Lophocarpa with sections Aestivales (1 species) and Dentatae (1 species). For all the considered species and varieties, the main distribution areas are given. A key has been compiled to determine the wild Adonis species distributed in Russia.

PROSPECTS FOR USING ASTRAGALUS ONOBRYCHIS AS A SOURCE OF NATURAL ANTIOXIDANTS

2021 ◽  
pp. 47-53
Author(s):  
Pozdnyakovа Т.А. ◽  
Kuleshovа Е.S. ◽  
Bubenchikov R.A.
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Chemical Interaction ◽  
Raw Materials ◽  
Natural Antioxidants ◽  
The Body ◽  
Plant Raw Materials ◽  
Total Antioxidant ◽  
Promising Source ◽  
The Relationship

The predominance of synthetic and genetically modified foods in the diet of a modern person often leads to disruption of natural metabolic processes and the accumulation of toxic free radicals in the body, which provokes the development of various diseases. Natural antioxidants from medicinal plants can help maintain health and stop the processes of peroxidation. In our opinion, Аstragalus onobrychis can become a promising source of natural antioxidants. The aim of this study was to establish the antioxidant activity in extracts from the herb Astragalus onobrychis. In order to obtain objective and reliable results during our research, we used two methods: the method of titrimetry and spectrophotometry of free radicals. The titrimetric method is based on the chemical interaction between an oxidizing agent (potassium permanganate) and natural antioxidants present in extracts from plant raw materials, since they exhibit the properties of reducing agents. The spectrophotometric method makes it possible to assess the value of antioxidant activity and the duration of the reaction by assessing the interaction of 2,2-diphenyl-1-picrylhydrazil with plant antioxidants. To establish the relationship between the antioxidant activity and the presence of phenolic compounds in the plant, the content of flavonoids and hydroxycinnamic acids in the herb Astragalus onobrychis was determined. As a result of the studies, the total antioxidant activity of extracts from the herb Astragalus onobrychis was established and it was revealed that its value depends on the type of extractant used, since this factor is associated with the content of various groups of natural antioxidants in the extraction. The data obtained indicate the prospects of using the herb Astragalus onobrychis as a source of natural antioxidants.

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