scholarly journals Identification of Salt and Drought Biochemical Stress Markers in Several Silene vulgaris Populations

2019 ◽  
Vol 11 (3) ◽  
pp. 800 ◽  
Author(s):  
Aleksandra Koźmińska ◽  
Alina Wiszniewska ◽  
Ewa Hanus-Fajerska ◽  
Monica Boscaiu ◽  
Mohamad Al Hassan ◽  
...  
Keyword(s):  
Salt Stress ◽  
Photosynthetic Pigments ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Total Phenolic ◽  
Enzymatic Antioxidants ◽  
Silene Vulgaris ◽  
Chlorophylls A And B ◽  
Monovalent Ions ◽  
Contrasting Habitats

This study attempted to determine short-term responses to drought and salt stress in different Silene vulgaris genotypes and to identify potential abiotic stress biochemical indicators in this species. Four populations from contrasting habitats were subjected to drought and three levels of salinity under controlled greenhouse conditions. The determination of several growth parameters after the stress treatments allowed for ranking the tolerance to stress of the four analyzed populations on the basis of their relative degree of stress-induced growth inhibition. This was then correlated with changes in the leaf levels of monovalent ions (Na+, Cl−, and K+), photosynthetic pigments (chlorophylls a and b, carotenoids), osmolytes (total soluble sugars, proline), and non-enzymatic antioxidants (total phenolic compounds and flavonoids). Despite the observed differences, all four populations appeared to be relatively tolerant to both stress conditions, which in general did not cause a significant degradation of photosynthetic pigments and did not generate oxidative stress in the plants. Drought and salinity tolerance in S. vulgaris was mostly dependent on the use of Na+ and K+ for osmotic adjustment under stress, a mechanism that appeared to be constitutive, and not stress-induced, since relatively high concentrations of these cations (without reaching toxic levels) were also present in the leaves of control plants. The inhibition of additional transportation of toxic ions to the leaves, in response to increasing external salinity, seemed to be a relevant mechanism of tolerance, specifically to salt stress, whereas accumulation of soluble sugars under drought conditions may have contributed to tolerance to drought.

scholarly journals Responses to Salt Stress in Portulaca: Insight into Its Tolerance Mechanisms

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1660
Author(s):  
Orsolya Borsai ◽  
Mohamad Al Hassan ◽  
Cornel Negrușier ◽  
M. Dolores Raigón ◽  
Monica Boscaiu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Salt Stress ◽  
Photosynthetic Pigments ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Total Phenolic ◽  
Saline Stress ◽  
Enzymatic Antioxidants ◽  
Stress Markers ◽  
Monovalent Ions

Climate change and its detrimental effects on agricultural production, freshwater availability and biodiversity accentuated the need for more stress-tolerant varieties of crops. This requires unraveling the underlying pathways that convey tolerance to abiotic stress in wild relatives of food crops, industrial crops and ornamentals, whose tolerance was not eroded by crop cycles. In this work we try to demonstrate the feasibility of such strategy applying and investigating the effects of saline stress in different species and cultivars of Portulaca. We attempted to unravel the main mechanisms of stress tolerance in this genus and to identify genotypes with higher tolerance, a procedure that could be used as an early detection method for other ornamental and minor crops. To investigate these mechanisms, six-week-old seedlings were subjected to saline stress for 5 weeks with increasing salt concentrations (up to 400 mM NaCl). Several growth parameters and biochemical stress markers were determined in treated and control plants, such as photosynthetic pigments, monovalent ions (Na+, K+ and Cl−), different osmolytes (proline and soluble sugars), oxidative stress markers (malondialdehyde—a by-product of membrane lipid peroxidation—MDA) and non-enzymatic antioxidants (total phenolic compounds and total flavonoids). The applied salt stress inhibited plant growth, degraded photosynthetic pigments, increased concentrations of specific osmolytes in both leaves and roots, but did not induce significant oxidative stress, as demonstrated by only small fluctuations in MDA levels. All Portulaca genotypes analyzed were found to be Na+ and Cl− includers, accumulating high amounts of these ions under saline stress conditions, but P. grandiflora proved to be more salt tolerant, showing only a small reduction under growth stress, an increased flower production and the lowest reduction in K+/Na+ rate in its leaves.

scholarly journals Variation in Chemicals and Growth Parameters of Taşköprü Garlic

2020 ◽  
Vol 8 (4) ◽  
pp. 847-854
Author(s):  
Nezahat Turfan
Keyword(s):  
Amino Acid ◽  
Bioactive Compounds ◽  
Soluble Protein ◽  
Amylase Activity ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Total Phenolic ◽  
Glucose Content ◽  
Proline Amino Acid ◽  
Β Carotene

Main goals of the present study were (1) to initially investigate the nutrient contents and bioactive compounds in the bulb and cloves of garlic, and (2) to study the growth parameter after planting. Garlic bulbs were firstly separated into three categories as pickled, big and small, while the big garlic cloves were also classified into three categories as big, small and central. Secondly, the garlic samples were analyzed before planting for their element profile, proline, soluble protein, free amino acid, β-carotene, lycopene, total phenolic, soluble sugars, SOD and α-amylase activities. Finally, the growth parameters were measured using the cultivated cloves and the pickled bulbs. According to the result, the highest soluble protein, N, phenolic, lycopene and α amylase activity (97.06 mg, 2.58%, 971 mg, 0.368 mg and 38.13 EU, respectively) were recorded in the biggest cloves. The highest proline, amino acid, glucose content (93.84 µmol, 23.54 mg, 230.89 mg, respectively) and K, P, S, Mg, Mn, Fe and Zn (21940 ppm, 7577 ppm, 12200 ppm, 504 ppm, 38.1ppm, 377,7ppm and 44.5 ppm, respectively) were found in the pickled bulb. The maximum level of β-carotene (0.282 mg), Ca, Cl and Sr (11260ppm, 818.7ppm and 47.9 ppm) were determined in the small bulbs. Based on the growth parameters of seedlings, the highest value of shoot and root length (39.12 cm and 24.11 cm respectively), the fresh weight of shoot and root (5.29 g and 4.54 g respectively) and dry weight of shoot and root (1.70 g and 1.24 g respectively) were noted with the big cloves. The results of the current study have indicated that the pickled cloves have higher macro and micro nutrients, proline, amino acid and glucose, while the big cloves of garlic have higher proline, phenolic, N%, lycopene and amylase activity. It can be said that the big cloves showed good value for the five bioactive compounds, but the pickled exhibited good value for the macro and micro element and glucose.

scholarly journals Effect of Salt Stress on Growth, Physiological Parameters, and Ionic Concentration of Water Dropwort (Oenanthe javanica) Cultivars

2021 ◽  
Vol 12 ◽  
Author(s):  
Sunjeet Kumar ◽  
Gaojie Li ◽  
Jingjing Yang ◽  
Xinfang Huang ◽  
Qun Ji ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Activities ◽  
Soluble Sugars ◽  
Limiting Factor ◽  
Enzymatic Antioxidants ◽  
High Accumulation ◽  
Oenanthe Javanica ◽  
Leaves And Roots ◽  
Water Dropwort ◽  
Number Of Branches

Salt stress is an important environmental limiting factor. Water dropwort (Oenanthe javanica) is an important vegetable in East Asia; however, its phenotypic and physiological response is poorly explored. For this purpose, 48 cultivars of water dropwort were grown hydroponically and treated with 0, 50, 100, and 200 mm NaCl for 14 days. Than their phenotypic responses were evaluated, afterward, physiological studies were carried out in selected sensitive and tolerant cultivars. In the present study, the potential tolerant (V11E0022) and sensitive (V11E0135) cultivars were selected by screening 48 cultivars based on their phenotype under four different levels of salt concentrations (0, 50, 100, and 200 mm). The results depicted that plant height, number of branches and leaves were less effected in V11E0022, and most severe reduction was observed in V11E0135 in comparison with others. Than the changes in biomass, ion contents, accumulation of reactive oxygen species, and activities of antioxidant enzymes and non-enzymatic antioxidants were determined in the leaves and roots of the selected cultivars. The potential tolerant cultivar (V11E0022) showed less reduction of water content and demonstrated low levels of Na+ uptake, malondialdehyde, and hydrogen peroxide (H2O2) in both leaves and roots. Moreover, the tolerant cultivar (V11E0022) showed high antioxidant activities of ascorbate peroxidase (APX), superoxide dismutase, peroxidase, catalase (CAT), reduced glutathione (GSH), and high accumulation of proline and soluble sugars compared to the sensitive cultivar (V11E0135). These results suggest the potential tolerance of V11E0022 cultivar against salt stress with low detrimental effects and a good antioxidant defense system. The observations also suggest good antioxidant capacity of water dropwort against salt stress. The findings of the present study also suggest that the number of branches and leaves, GSH, proline, soluble sugars, APX, and CAT could serve as the efficient markers for understanding the defense mechanisms of water dropwort under the conditions of salt stress.

scholarly journals Responses to Drought in Seedlings of European Larch (Larix decidua Mill.) from Several Carpathian Provenances

Forests ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 511
Author(s):  
Ioana M. Plesa ◽  
Mohamad Al Hassan ◽  
Sara González-Orenga ◽  
Adriana F. Sestras ◽  
Oscar Vicente ◽  
...  
Keyword(s):  
Drought Stress ◽  
Phenolic Compounds ◽  
Early Detection ◽  
Photosynthetic Pigments ◽  
Soluble Sugars ◽  
Larix Decidua ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
European Larch ◽  
Total Soluble Sugars

European larch (Larix decidua Mill.) has been reported either as more tolerant or as more sensitive to drought than conifers with perennial leaves. Previous studies have revealed that Carpathian populations of European larch display a high genetic variability. A comparative study of the responses of these populations to drought stress at the seedling stage might allow the identification of drought tolerant genotypes and reliable drought stress biomarkers, which could be eventually used for the early detection of drought effects in larch, not only under control greenhouse conditions, but also in their natural stands. Growth responses were analyzed in larch seedlings from six Romanian Carpathian populations, submitted to one month of mild drought stress under controlled conditions. Levels of photosynthetic pigments (chlorophylls a and b, and carotenoids), osmolytes (proline and total soluble sugars), monovalent cations (Na+ and K+), and malondialdehyde (MDA) and non-enzymatic antioxidants (total phenolics and flavonoids) were compared with control treatments and between populations. Growth and the pattern of the biochemical responses were very similar in the six populations. Drought stress lead to stem length decrease in all population, whereas reduction of fresh weight of needles was significant only in one population (BVVC), and reduction of water content of needles in two populations (BVVC and GuHo). The optimal biochemical traits for an early detection of drought symptoms in this species is the increase—in most populations—of total soluble sugars, MDA, and total phenolic compounds, whereas K+ reduction was significant in all populations. Photosynthetic pigments remained unchanged, except for the Anin population where they were reduced under stress. Multivariate principal component and hierarchical clustering analyses confirmed the impact of drought in the growth and physiology of European larch, and revealed that the humidity of the substrate was positively correlated with the growth parameters and the levels of K+ in needles, and negatively correlated with the levels of MDA, total soluble sugars, total phenolic compounds, and flavonoids in needles.

scholarly journals Antioxidant Enzymatic Activities and Growth Response of Quinoa (Chenopodium quinoa Willd) to Exogenous Selenium Application

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 719
Author(s):  
Ahlam Khalofah ◽  
Hussein Migdadi ◽  
Ehab El-Harty
Keyword(s):  
Water Content ◽  
Photosynthetic Pigments ◽  
Relative Water Content ◽  
Defense Mechanism ◽  
Higher Plants ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Low Concentrations ◽  
Relative Water ◽  
High Concentrations

Selenium is a trace element essential to many organisms, including higher plants. At low concentrations, it enhances growth and development; however, it is toxic at high concentrations. The development of crops with proper levels of selenium will be worth for both nutrition and Se-based therapeutics. This study aimed to investigate the morphological, physiological, and biochemical responses of the quinoa plant to 0, 2.5, 5, 10, and 20 mg/L of Na2SeO3·5H2O. Selenium at low concentrations (2.5 and 5 mg/L), quinoa plant showed a significant increase of growth parameters, relative water content, photosynthetic pigments, proline, total soluble sugars, and antioxidant enzymes activities as (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD, ascorbate peroxidase (APX), and glutathione reductase (GR)), and contents of malondialdehyde (MDA) and H2O2 were reduced. However, high concentrations (10 and 20) mg/L caused a decrease in plant growth parameters, relative water content, and photosynthetic pigments. In contrast, excess selenium increased the oxidative stress monitored by hydrogen peroxide and lipid peroxidation levels. The enzymatic antioxidant system responded to the selenium supply significantly increased. Osmolytes compounds, such as total sugars and proline, increased in selenium-treated plants. The increase in these osmolytes compounds may show a defense mechanism for the osmotic readjustment of quinoa plants to mitigate the toxicity caused by selenium. This study shows the morphological and physiological responses that must be considered for success in the sustainable cultivation of quinoa plants in environments containing excess selenium.

scholarly journals Mitigation of Salinity Stress Effects on Broad Bean Productivity Using Calcium Phosphate Nanoparticles Application

Horticulturae ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 75
Author(s):  
Amira K. Nasrallah ◽  
Ahmed A. Kheder ◽  
Maimona A. Kord ◽  
Ahmed S. Fouad ◽  
Mohamed M. El-Mogy ◽  
...  
Keyword(s):  
Salt Stress ◽  
Calcium Phosphate ◽  
Salinity Stress ◽  
Agricultural Sector ◽  
Broad Bean ◽  
Growth Parameters ◽  
Saline Water ◽  
Soluble Sugars ◽  
Higher Plant ◽  
Plant Yield

Water salinity is one of the major abiotic stresses, and the use of saline water for the agricultural sector will incur greater demand in the coming decades. Recently, nanoparticles (NPs) have been used for developing numerous plant fertilizers as a smart and powerful form of material with dual action that can alleviate the adverse effects of salinity and provide the plant with more efficient nutrient forms. This study evaluated the influence of calcium phosphate NPs (CaP-NPs) as a soil fertilizer application on the production and bioactive compounds of broad bean plants under salinity stress. Results showed that salinity had deleterious effects on plant yield with 55.9% reduction compared to control. On the other hand, CaP-NPs dramatically improved plant yield by 30% compared to conventional fertilizer under salinity stress. This improvement could be attributed to significantly higher enhancement in total soluble sugars, antioxidant enzymes, proline content, and total phenolics recorded use of nano-fertilizer compared to conventional use under salt stress. Additionally, nano-fertilizer reflected better mitigatory effects on plant growth parameters, photosynthetic pigments, and oxidative stress indicators (MDA and H2O2). Therefore, our results support the replacement of traditional fertilizers comprising Ca2+ or P with CaP-nano-fertilizers for higher plant productivity and sustainability under salt stress.

scholarly journals Screening for drought tolerance in cultivars of the ornamental genusTagetes(Asteraceae)

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2133 ◽  
Author(s):  
Raluca Cicevan ◽  
Mohamad Al Hassan ◽  
Adriana F. Sestras ◽  
Jaime Prohens ◽  
Oscar Vicente ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Drought Tolerance ◽  
Plant Growth ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Stem Length ◽  
Total Phenolic ◽  
Total Soluble Sugars ◽  
Drought Tolerant ◽  
Components Analysis

Drought tolerance was evaluated in twelve cultivars of three ornamentalTagetesspecies (T. patula,T. tenuifoliaandT. erecta). A stress treatment was performed by completely stopping watering of plants maintained in controlled greenhouse conditions. After three weeks, several plant growth parameters (stem length (SL), fresh weight (FW) and water content (WC)), photosynthetic pigments (chlorophylls and carotenoids (Car)), osmolytes (proline (Pro), glycine betaine (GB) and total soluble sugars (TSS)), an oxidative stress maker (malondialdehyde (MDA)) and antioxidants (total phenolic compounds (TPC) and total flavonoids (TF)) were measured. Considerable differences in the evaluated traits were found among the control and drought-stressed plants. Drought stress generally caused a marked reduction in plant growth and carotenoid pigments, and an increase in soluble solutes and oxidative stress. For most cultivars, proline levels in stressed plants increased between 30 and 70-fold compared to the corresponding controls. According to the different measured parameters, on averageT. erectaproved to be more tolerant to drought thanT. patulaandT. tenuifolia. However, a considerable variation in the tolerance to drought was found within each species. The traits with greater association to drought tolerance as well as the most tolerant cultivars could be clearly identified in a principal components analysis (PCA). Overall, our results indicate that drought tolerant cultivars ofTagetescan be identified at early stages using a combination of plant growth and biochemical markers.

scholarly journals Responses to Drought and Salinity in the Endangered Species Ligularia sibirica (L.) Cass.

2016 ◽  
Vol 73 (2) ◽  
pp. 252
Author(s):  
Andreea Natalia Matei ◽  
Mohamad Al Hassan ◽  
Monica Boscaiu ◽  
Valeriu Alexiu ◽  
Oscar Vicente
Keyword(s):  
Oxidative Stress ◽  
Salt Stress ◽  
Growth Parameters ◽  
Anthropogenic Activities ◽  
Soluble Sugars ◽  
Stress Factors ◽  
Plant Distribution ◽  
Negative Effects ◽  
Negative Effect ◽  
Stress Biomarker

The negative effects of environmental stress factors on plant distribution and survival are increasing due to climate change and anthropogenic activities. We have analysed some responses to abiotic stress in Ligularia sibirica, a postglacial relict that is critically endangered in Europe. L. sibirica seedlings were subjected to water or salt stress treatments in the greenhouse. After the treatments, plant material was harvested and several growth parameters were measured; leaf contents of common osmolytes, the degree of oxidative stress affecting the plants and the level of antioxidant phenolic compounds were also determined. Both, drought and, especially, salt stress had a negative effect on the growth of L. sibirica plants. Treated plants showed an increase in proline (Pro) and total soluble sugars (TSS) levels, stronger under salt stress. Malondialdehyde (MDA, an oxidative stress biomarker) contents almost doubled, and antioxidant phenolics increased significantly in salt-stressed, but not in water-stressed plants. Pro accumulation can be used as a salt and drought stress biomarker in L. sibirica and, together with TSS, likely contributes to osmotic adjustment under stress. Increase of antioxidant phenolics appears to partly compensate the salt-induced generation of oxidative stress.

scholarly journals Comparative Studies on the Physiological and Biochemical Responses to Salt Stress of Eggplant (Solanum melongena) and Its Rootstock S. torvum

Agriculture ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 328
Author(s):  
Marco Brenes ◽  
Jason Pérez ◽  
Sara González-Orenga ◽  
Andrea Solana ◽  
Monica Boscaiu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Specific Activity ◽  
Soluble Sugars ◽  
Solanum Melongena ◽  
High Salt ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Enzymatic Antioxidants ◽  
Biochemical Responses ◽  
Physiological And Biochemical

This study investigated the physiological and biochemical responses to salinity stress of Solanum melongena and its wild relative, Solanum torvum, commonly used as eggplant rootstock. Young plants of both species were watered during 25 days with NaCl aqueous solutions at the following four final concentrations: 0 (for the controls), 100, 200, and 300 mM. Plant growth parameters, photosynthetic pigments content, monovalent ion concentrations in roots and leaves, leaf levels of osmolytes (proline and total soluble sugars), oxidative stress markers (MDA and H2O2), non-enzymatic antioxidants (total phenolic compounds and total flavonoids), and enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione reductase) were determined after the stress treatments. Salt-induced growth reduction was more significant in S. melongena than in S. torvum, especially at high salt concentrations, indicating a (slightly) higher salt tolerance of the wild species. The mechanisms of tolerance of S. torvum were partly based on the active transport of toxic ions to the leaves at high external salinity and, presumably, a better capacity to store them in the vacuoles, as well as on the accumulation of proline to higher concentrations than in the cultivated eggplant. MDA and H2O2 contents did not vary in response to the salt treatments in S. torvum. However, in S. melongena, MDA content increased by 78% when 300 mM NaCl was applied. No activation of antioxidant mechanisms, accumulation of antioxidant compounds, or increase in the specific activity of antioxidant enzymes in any of the studied species was induced by salinity. The relatively high salt tolerance of S. torvum supports its use as rootstock for eggplant cultivation in salinized soils and as a possible source of salt-tolerance genes for the genetic improvement of cultivated eggplant.

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