ПОКАЗАТЕЛИ ЕСТЕСТВЕННОЙ РЕЗИСТЕНТНОСТИ КОРОВ ЧЕРНО-ПЕСТРОЙ ПОРОДЫ В ЗАВИСИМОСТИ ОТ УРОВНЯ МОЛОЧНОЙ ПРОДУКТИВНОСТИ

Author(s):  
Yu.M. SMIRNOVA ◽  
A.V. PLATONOV ◽  
Т.В. СЕДУНОВА ◽  
А.Г. КУДРИН

Изучили влияние уровня продуктивности коров на показатели естественной резистентности организма на 3 группах коров черно-пестрой породы, разделенных по уровню удоя за предыдущую лактацию методом сбалансированных групп. I группа включала животных с продуктивностью до 6000 кг, II от 6001 до 8000, III от 8001 кг и более. Был проведен анализ продуктивных и воспроизводительных качеств коров, а также изучены морфо-биохимические и иммунологические показатели крови, где определяли содержание общего белка, его фракций, фагоцитарную активность нейтрофилов. Установили, положительную взаимосвязь между продуктивностью животных за предыдущую лактацию и уровнем продуктивности за 100 дней последующей лактации. Наблюдается удлинение сервис-периода от 94 до 110 дней и количества доз на оплодотворение животных от 1,3 до 1,8. Рост молочной продуктивности влечет за собой снижение поглотительной способности нейтрофилов (фагоцитарное число, фагоцитарный индекс и фагоцитарная активность). У животных III группы в сухостойный период, и в период раздоя установлено снижение фагоцитарного числа по сравнению с показателями коров I и II групп. Во II группе отмечается наибольшее количество фагоцитирующих нейтрофилов к общему числу подсчитанных (23,67). Увеличение молочной продуктивности приводит к снижению содержания -глобулинов в крови сухостойных коров. Полученные данные свидетельствуют об обратной связи между естественной резистентностью организма и уровнем молочной продуктивности коров.We analyzed the influence of cows productivity level on indicators of organisms natural resistances in 3 groups of black pied cattle, divided according to the level of yield accumulated during previous lactation, through the method of balanced groups. Group I included animals with productivity level up to 600 kg, II from 6.001 kg to 8000 kg, III from 8001 kg and more. The analysis of productive and reproducible qualities of cows was conducted. Also, we studied morpho-biochemical and immunological parameters of blood, which determined the content of total protein, its fractions, phagocytic activity of neutrophils. We established a positive relationship between the productivity of animals during the previous lactation and the level of productivity during 100 days of subsequent lactation. There is an extension of the service period from 94 to 110 days and the number of doses for animals fertilization increased from 1.3 to 1.8. The growth of milk productivity entails a decrease in the absorption capacity of neutrophils (phagocytic number, phagocytic index, and phagocytic activity). Among animals of group III, during the dry and milking periods, a decrease, in comparison with the indicators of cows from groups I and II, of the phagocytic number was found. In group II, there is a highest number of phagocytic neutrophils in relation to its counted total number (23.67). The increase of milk productivity leads to a decrease of -globulins in the blood of dry cows. Obtained data indicate an inverse relationship between the natural resistance of the organism and the level of cows milk productivity.

2019 ◽  
Vol 72 (3) ◽  
pp. 401-404
Author(s):  
Oleksandr V. Avdeev ◽  
Roksana O. Drevnitska ◽  
Alina B. Boykiv ◽  
Oksana Ya. Vydoinyk

Introduction: Taking into account the patho-immune mechanisms of formation of inflammatory process in periodontium, there is a necessity for in-depth study of the pathogenesis of periodontal diseases from the position of changes in the reactivity of the organism. The aim: Carrying out a comparative estimation of phagocytic activity of leukocytes of animals with inflammation in periodontium with altered reactivity. Materials and methods: Experiments were performed on 30 white rats: Group I - 10 white rats with hypoergic reaction; Group ІІ - 10 white rats with hyperergic reaction; Group III - 10 white rats with normergic reaction - control group. Slaughter and blood sampling under thiopental anesthesia was performed 7 days after the beginning of the experiment. The percentage of phagocytic leukocytes - phagocytic index, phagocytic number were determined and the index of phagocytic activity was calculated. Results: The phagocytic index (Fi,%) decreased by 2,09 times (p <0,05) at hypoergic, it increased by 1,37 times (p <0,05) at hyperergic; the index of phagocytic activity (IFA) decreased by 1.96 times at hypoergic (p <0.05); growth was 1.94 times (p <0.05) in the hyperergic group of animals. In both experimental groups, the phagocyte number (Fu) increased by 6.25% and 41.7%, with hypoergic and hyperergic, respectively. Conclusions: Different directions of changes of the phagocytic activity were observed: increase of these parameters at hyperergic and decrease at hypoergic. An increase in phagocyte number was likely to indicate some autonomy of the process of phagocytosis and independence from the reactivity of the organism.


2020 ◽  
Author(s):  
Zbigniew Kamocki ◽  
Joanna Matowicka ◽  
Anna Jurczuk ◽  
Anna Milewska ◽  
Anna Kamocka ◽  
...  

Abstract Background:The aim was to determine the phagocytic activity of thrombocytes in patients with gastric cancer and to assess the effect of oral and parenteral preoperative glutamine-based immunonutrition on nutritional status, thrombocyte phagocytic activity and early postoperative outcomes. Methods:Patients suffer from invasive gastric cancer had been treated with preoperative immunonutrition with glutamine and they were compared to patients without nutritional treatment. Nutritional status, percentage of weight loss and BMI were assessed. Levels of total protein, albumin, cholesterol, triglycerides, platelets and their phagocytic ability were measured twice. Postsurgical complications were assessed via Claven-Dindo classification. Results:Group I-20 patients with an oral glutamine, Group II-38 patients received an intravenous glutamine. Group III-25 patients did not receive preoperative immunonutrition. 47% patients Group I, 54% patients Group II and 33% patients Group III were malnourished. In Group I, percentage of phagocytizing platelet (%PhP) was 1.1 pre- and 1.2 postoperatively. Phagocytic index (PhI) was 1.0 and 1.1. In Group II, %PhP was 1.1 and 1.2. PhI was 1.0 and 1.1. In Group III %PhP was 1.0 and1.2. PhI was 1.0 and 1.1. An increase in triglyceride level was observed in both immunonutrition groups. There was a fall in total protein, albumin level in Group II. In Group III there was a decline in total protein, albumin and cholesterol level. Total platelet count, and PhI was increased in both immunonutrition groups. There was also a rise in %PhP in Group II. In Group III there was no change in blood plateles level, %PhP and PhI. Complications rate was 53% in Group I, 29 % in Group II, 40% in Group III. Conclusions:In invasive gastric cancer, laboratory nutritional parameters are significantly reduced, causing malnutrition in 45.7% of patients. Oral glutamine supplementation inhibited the postoperative decline in protein metabolism parameters, however, this did not affect the reduction of the percentage of postoperative complications. Glutamine used preoperatively significantly reduced the percentage of serious surgical complications, regardless of the way it was supplemented. Patients with invasive gastric cancer have a significant decrease in platelet phagocytic activity. Immunonutrition based on intravenous form of glutamine allows to improve the phagocytic activity of platelets.


Author(s):  
Ch. N. Sambyla ◽  
N. M. Bessonova ◽  
R. B. Chysyma

The Republic of Tyva is a region in the geographical center of Asia, which located at the junction of the Siberian taiga and Central Asian desert-steppe landscapes, in a wide band of mountains and intermountain plains. The mountain-forest area of Tyva has long been considered a natural habitat for antler deer, one of which is the maral (Cervus elaphus sibiricus). In order to restore maral breeding and increase the number of marals in the former limits, marals of the Altai-Sayan breed have been imported to the Republic of Tyva from the Republic of Altai. The preservation of productive traits, reproductive function and the realization of the genetic potential of animals introduced to new climatic conditions largely depends on the ability of these animals to adapt to existing environments. We have assessed in this paper the natural resistance of the marals of Altai-Sayan breed during introduction in the Tyva Republic in comparison with the indicators of the marals have been bred in the Altai Republic. The researches have been carried out in 2019. The research material was blood samples of marals of Altai-Sayan breed imported to the Republic of Tuva (n=27) and marals of the same breed bred in the Republic of Altai (n=17). Studies have shown some deviations in the blood leukogram of imported marals, such as a decrease in the number of eosinophils and rod nuclear neutrophils (P < 0,001), the increase in the number of segmented nuclear neutrophils and lymphocytes (P < 0,05). The number of monocytes have been increased in 18,6 times compared to the Altai marals. The change in the number of monocytes exceeded the physiological norm by 24,7 %. The indicator of adaptation evaluation in imported marals had higher values (6,8), which were in 1,7 times higher than in marals bred in the Altai Republic (4,1), which indicates the intensity of adaptive mechanisms in imported animals during adaptation. Analysis of phagocytic activity and phagocytic index revealed intensive phagocytosis in imported marals: phagocytic activity – by 12,4 % (P < 0,05), phagocytic index – by 5,1 %, and the increase in the content of lysosomal and cationic proteins by 12,8 % (P < 0,05). Analysis of the bactericidal activity of blood serum has shown its lower level in imported animals (45,97±1,36 %), compared with marals of the same breed bred in the Altai Republic (52,19±2,15 at P < 0,05). Thus, according to most indicators of natural resistance marals of Altai-Sayan breed imported to Tyva have a fairly high level of natural protection, which indicates that they can be adapted to the natural climatic and feeding conditions of the Republic of Tyva.


Author(s):  
L. Bondarenko

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets


2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e18079-e18079
Author(s):  
Anna Yu. Ardzha ◽  
Ekaterina V. Verenikina ◽  
Elena Yu. Zlatnik ◽  
Vera P. Nikitina ◽  
Oksana E. Zhenilo ◽  
...  

e18079 Background: Immunotherapy in oncology has now proven effective, but standard approaches have not yet been defined. Ways to optimize and increase the effectiveness of treatment with immunomodulators, in particular interferon-gamma preparations, in such patients remain an urgent problem of gynecological oncology. The purpose of the study was to improve direct results of ovarian cancer treatment. Methods: The study included patients aged 50-77 years with inoperable ascitic ovarian cancer (verified by cytological examination of ascitic fluid), stage IIIC-IV, receiving neoadjuvant chemotherapy (CT) in combination with immunotherapy with interferon-gamma (IFNγ). Group I – 26 patients, CT with carboplatin (AUC-6), paclitaxel (175 mg/m2); group II – 22 patients, chemoimmunotherapy and intramuscular IFNγ (ingaron) - 500 000 IU on day 1, 1 million IU on days 2,3,5, a similar CT on day 4; group III – 24 patients, chemoimmunotherapy and intraperitoneal IFNγ (ingaron) - 500 000 IU on day 1, 1 million IU on days 2,3,5, a similar CT on day 4. Patients received on average 2-3 therapy cycles. Results: Progression was registered only in group I in 3.8%; complete response in patients without IFNγ – 7.8%, with intramuscular and intraperitoneal IFNγ – 27.3% and 37.5% (p≤0.05) respectively. Surgical treatment followed in all patients, with total surgeries in 87.5% of patients with intraperitoneal IFNγ. DNA cytometry showed the minimal number of aneuploid tumors in patients with intraperitoneal IFNγ (16.6%), while in patients without immunotherapy 38.4%. The DNA index statistically significantly decreased by 1.3 times in patients with intraperitoneal IFNγ compared with patients without IFNγ (1.11±0.01% vs. 1.4±0.05% respectively) (p≤0.05). Levels of CD3+CD4+ cells elevated by 1.2 times (from 36.2±1.6 to 44.9±3.78%, p≤0.05) in patients with intramuscular IFNγ; intraperitoneal IFNγ caused an increase in CD3+ lymphocytes by 1.3 times (from 62.1±2.8 to 77.9±2.94%, p < 0.05) and CD3+CD4+ by 1.4 times (from 36.2±1.6 to 50.6±5.9%, p≤0.05). Conclusions: Interferon-gamma (ingaron) in combination with CT improves direct results of the treatment; intraperitoneal injections of interferon-gamma demonstrated better outcomes and tolerance confirmed by immunological and DNA cytometric parameters.


2020 ◽  
pp. 427-433
Author(s):  
M.A. YUSUPOVA ◽  
◽  
K.I. ISMOILOV ◽  

Objective: To study the features of clinical manifestations, cellular and humoral immunity of newborns with intrauterine mixed infections (IUMI). Methods: A comprehensive survey of 45 infants with IUMI was carried out. Depending on the classification of IUMI they were divided into 3 main groups: group I – 24 patients (53%) with the viral-bacterial association, group II – 12 patients (27%) with the viral-viral association, and group III – 9 patients (20%) with the viral-parasitic association. The control group consisted of 10 newborns born from uninfected, somatically healthy mothers. Diagnosis of IUMI was based on the detection of specific antibodies of the IgA, IgG and IgM classes, as well as phagocytic activity and phagocytic index of leukocytes. In addition, general clinical, biochemical, bacteriological and instrumental research methods have been conducted. Results: The blood serum IgA and IgM indices in patients of the main group were significantly higher, and the mean IgG values were lower compared to the control group. Analysis of cellular immunity parameters in the main group showed a decrease in the number of mature T-lymphocytes (CD3), B-lymphocytes (CD20), the number of T-helpers and cells that produce IL-2 in the peripheral blood, compared with the control group. In the main group, there was also an increase in the number of apoptosis cells (CD95), cells with high cytotoxic activity (CD25, CD71) and the percentage of natural killer cells (CD16). A decrease in the phagocytic activity and phagocytic index of neutrophils was recorded, which indicates the insufficiency of the nonspecific component of immunity. Conclusions: In newborns, various changes were found both on the part of specific and nonspecific components of immunity. This indicates the development of secondary immunodeficiency in this category of patients and makes it necessary to add to the main treatment of immune corrective therapy. Keywords: Congenital infections, mixed intrauterine infections, TORCH syndrome, opportunistic infections, secondary immunodeficiency.


2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Adryan Fristiohady ◽  
Wahyuni Wahyuni ◽  
Muhammad H Malaka ◽  
Dewiyanti Madu ◽  
Dayatriana Muthalib ◽  
...  

Immunomodulator is a substance that increases or suppresses the immune response through the certain mechanism. The marine sponge Xestospongia sp. has immunomodulatory activity by increasing phagocytic activity. In addition, the phagocytic activity is affected by CD4+ and CD14 cells levels. Thus, this study aims to investigate the effect of Xestopongia sp. extract toward CD4+ and CD14 cells level in model rat. Animals were divided into 4 groups (n=5) and treated for 7 days, as follow: Group I (Ethanolic extract of Xestospongia sp. dose of 300 mg/KgBW); Group II (Ethanolic extract of Xestospongia sp. dose of 400 mg/KgBW); Group III (Phylantus niruri extract); and Group IV (0.5% Na CMC). On day 8, animals were infected with Staphylococcus aureus intraperitoneally. And the blood was collected by cardiac puncture and assayed with ELISA kit CD4 (elabscience®) and ELISA kit CD14 (elabscience®). Ethanolic extract of Xestospongia sp. provided high levels of both CD4+ and CD14 cells (Group II) compared to baseline (Group IV)  (p<0.05). Group I provided similar activity to group III (p>0.05) and Group II provided significant activity with higher levels of CD4+ and CD14 cells compared to group III (p<0.05). In conclusion, both doses of Xestospongia sp. extract provide activity as immunomodulator by increasing CD4+ and CD14 cells levels, yet dose of 400 mg/KgBw provides the higher immunomodulatory activity.


2016 ◽  
Vol 18 (3(71)) ◽  
pp. 244-247
Author(s):  
G. Sobko

The article contains the results ofthe experimental researches of the influence of preparation «Antymast», is made from natural bee raw material, on the indicators of neutrophils granulocytes phagocytosis of cows blood, which have subclinical form of mastitis.The research was conducted during the spring period on two groups of cows of Black–Spotted Dairy breeds (2–3 lactation) 5 – 7 animals in each group.The cows before starting the investigations were diagnosed with the help of viscose metric express method for subclinical forms of mastitis: control group – clinically healthy animals, research group – with signs of subclinical mastitis.Cows from the research group were administered intracisternally with one injection of syringe tube (13 ml) three times at intervals of 24 hours of preparation «Antymast»in the affected udder quarters, half of the therapeutic dose was prophylactically injected into the healthy quarter of breast.It was established that in cows, sick on subclinical form of mastitis, was observed the activation of phagocytic activity of blood granulocytes neutrophils against decrease in phagocytic index (p < 0,05) and the number and increase (p < 0,05) spontaneous NBT test.Intracisternal introduction of preparation «Antymast» to sick cows contributed to the normalization of phagocytic activity of blood neutrophils and increase their absorption capacity and the reduction of NBT test (p < 0,05). 


Author(s):  
S.A. OVODKOV ◽  
Ah.S. DELYAN

Изучено влияние способа содержания и сезона отела на лактационную деятельность голштинизированных коров-первотелок черно-пестрой породы. Исследования проведены в условиях сельскохозяйственного предприятия Тверской области на 4 группах животных: I группа — привязное содержание, летние отелы; II — привязное содержание, зимние отелы; III — беспривязное содержание, летние отелы; IV — беспривязное содержание, зимние отелы. Максимальные удои у коров I, III и IV групп были получены на 3-м мес лактации, II — на 2-м. Удой в I, II, III и IV группах составил, соответственно, 7714, 8717, 7682 и 7930 кг молока за 305 дней лактации. Удой коров за первые 3 мес в % к удою за 305 дней лактаций: I группа — 33,7%, II — 35,8, III — 34,4, IV — 35,9%. Максимальный суточный удой в I группе равнялся 30,8 кг, во II — 36,5, в III —29,7 и в IV — 35,3. Среднесуточный удой за 305 дней лактации в I группе составил 21,2 кг, во II — 23,6, в III группе — 19,4 и в IV группе — 21,7. Коэффициент постоянства лактации в I II III и IV группах равнялся 93,2, 94,2, 89,2 и 98%, соответственно. Независимо от способа содержания более высокие показатели молочной продуктивности и устойчивости лактационной деятельности имели коровы, отелившиеся в зимний период.The influence of the method of keeping and calving season on the lactation activity of Holstein cows-heifers of black-and-white breed is studied. Studies were conducted in the agricultural enterprise of the Tver region on 4 groups of animals: I group — tethered content, summer calving; 2 — tethered content, winter calving; III — loose content, summer calving; 4 — loose content, winter calving. Maximum milk yield in cows I and III and IV groups were obtained on the 3rd month of lactation, the second — at the 2nd. Milk Yield in I, II and III and in IV groups made, respectively, 7714, 8717, 7682 and 7930 kg of milk for 305 days of lactation. Milk yield of cows for the first 3% to milk yield for 305 days of lactation: group I-33,7%, II — 35,8, III-34,4, IV-35,9%. The maximum daily yield in group I was 30.8 kg, in group II — 36.5, in group III — 29.7 and in group IV — 35.3. The average daily yield for 305 days of lactation in group I was 21.2 kg, in group II — 23.6, in group III — 19.4 and in group IV — 21.7. The coefficient of persistence of lactation in I, II, III and IV groups was equal to 93.2 per cent, 94,2, to 89.2 and 98%, respectively. Regardless of the method of maintenance, cows calving in winter had higher indicators of milk productivity and stability of lactation activity.


2020 ◽  
Vol 59 ◽  
pp. 17-25
Author(s):  
S. F. Antonenko

The scientific experiment was carried out in two stages at State enterprise pilot farm “Hontarivka”, Vovchansk district, Kharkiv region. First stage. In a retrospective analysis of the rearing calf intensity from 3 to 6 months revealed that the highest live weight at 6-month age had animals of group III, .which is probably 47.0 kg or 34.6% and 23.0 kg or 14.4% (P < 0.001 in both comparison cases) outperformed peers I and II groups. A similar trend was observed in the analysis of live weight of heifers at 9 and 12-month age. Live weight at insemination in the higher growth group also had better rates of 4.0 and 3.7%, respectively, in animals of groups I and II (P < 0.001–0.05). It should be noted that the required live weight for insemination at heifers at growth rates of up to 500 g was reached in only 20.3 months, however, in the other two groups, the insemination age decreased by 1.5 and 1.7 months. Firstcalf heifers from group III also had the highest milk expectancy of 170.0 and 123.0 kg, or 3.1 and 2.3%, respectively, then animals in groups I and II. In the age period from 6 to 9 months it was found that at 9-month age heifers of group III had a higher live weight against animals of I and II groups respectively by 39.0 and 16.0 kg or 18.8 and 7.0% (P < 0.001 in both comparison cases). Intergroup differences by this indicator at 12-month age hiefers decreased to 35.0 and 8.0 kg, respectively, or 13.0 and 2.7% (P < 0.001 in both cases of comparison), which provided an advantage for the live weight of group III over I and II groups during the first fruitful insemination. Whereas the age of first fruitfull insemination, on the contrary, was lower at heifers of group III at 49 and 34 days than at groups I and II heifers. The highest milk productivity was also found at firstcalf heifers of group III at 634 kg or 12.3% (P < 0.001) and at 137 kg or 2.5% against animal I and II groups. The heifer live weight at the period from 9 to 12 months corresponded to the established differences at other age periods generally. And 12-month age calves of group III also significantly exceeded the analogues of group I by 38.0 kg or 14.0% (P < 0.001) and peers of group II – by 23.0 kg or 8.0% (P < 0.001). The live weight at fruitfull insemination of group III heifers was the largest and the difference compared with animals of group I was 21.0 kg or 5.3% (P < 0.01) and group II – 19.0 kg or 4.8% (P < 0.001). Group III animals also had the highest milk productivity, which is likely to be higher than group I peers by 751 kg or 14.5% (P < 0.001) and group II animals by 552 kg or 10.3% (P < 0.001). Second stage. It was found that during the experiment, live weight of free-stall keeping heifers were outnumbered by peers. In particular, the live weight of group I animals at the first month of the experiment was less by 8.0 kg or 2.9%, the second by 15.0 kg or 4.7% (P < 0.05) and the third by 19.0 kg or 5.4% against peers of group II. A similar pattern was observed in the estimation of intensive growth of these animals. However, at the end of the experiment, the heifers of the experimental group were 7.0 cm or 5.9% (P < 0.001) higher in the withers, 7.0 cm or 5.6% (P < 0.001) in the back, and the sacrum – 6.0 cm or 4.7% (P < 0.001). They showed deeper breasts by 3.0 cm or 4.9% (P < 0.01), larger breast widths – by 2.0 cm or 5.4% (P < 0.01), width in macaques – by 2.0 cm or 4.9% (P < 0.01), oblique torso length – 8.0 cm or 5.8% (P < 0.001), chest circumference 9.0 cm or 5.6% (P < 0.001) and girth – 1.0 cm or 6.1% (P < 0.01). It should be noted that from the second month of the research the animal number the free-stall keeping animal number, which ready for insemination, was more at 2.3 times or 5 goals than stall keeping heifers, and their live weight was more at 8 kg or 3.0%. With age, the differences between the groups gradually increased and by the third month reached 8 goals or 2.6 times and 19.0 kg or 5.7%.


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