scholarly journals Activity of Antibiotic Bacterial Isolate Kp13 and Cell Leakage Analysis of Escherichia coli Bacteria

2018 ◽  
Vol 16 (2) ◽  
pp. 137
Author(s):  
Alfian Syarifuddin ◽  
Nanik Sulistyani ◽  
Kintoko Kintoko
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Bacterial Isolate ◽  
Inhibition Zone ◽  
Atomic Absorption Spectrophotometry ◽  
Uv Spectrophotometry ◽  
Active Fraction ◽  
Ion Release ◽  
Escherichia Coli Bacteria ◽  
Cell Leakage

A bacterium is one of microorganisms that can produce secondary metabolite, such as antibiotic. KP13 isolate is a bacterium isolated from Melaleucaleuca dendron L rhizosphere. The aim of this study is to know the eff ect of the most active fraction antibacterium of KP13 isolate toward Escherichia coli bacterium and the ability to caused cell leakage. Extraction of antibiotic was conducted by ethyl acetate. Extract is fractioned with a n-heksan-etilacetate solvent gradient another uses a solvent methanol. Each fraction is done by KLT and grouped based on RF value. A fraction group is compare by inhibition eff ectiveness. Then, the Minimum Inhibitory Concentration (MIC) from the most active group is tested. Cell leakage analysis was performed using UV spectrophotometry to detect the release of nucleic acid and protein. Atomic Absorption Spectrophotometry (AAS) was used to detect ion release of K+ and Ca2+ from. cell of bacteria. The results showed that the most active fraction against Escherichia coli was the fraction number 1 with MIC value of 5% and inhibition zone of 7.33±0.58 mm.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine polmifolia (L.) Merr) TERHADAP Salmonella typhi dan Escherichia coli

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Nofita Nofita ◽  
Keyword(s):  
Escherichia Coli ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Skin Infections ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Agar Diffusion ◽  
Escherichia Coli Bacteria

Dayak onion (Eleutherine polmifolia (L.) Merr) was a typical plant of Kalimantan. Empirically dayak onion (Eleutherine polmifolia (L.) Merr) used by the comunity to treat skin infections. The purpose of this study was to determine the antibacterial activity of ethanol extract of dayak onion power against Salmonella typhi and Escherichia coli bacteria. The highest concentration used was 100%, and the lowest concentration was 0,5%. As a control of cloramfenikol for Salmonella typhi and ciprofloxacin for Escherichia coli bacteria. This research method used agar diffusion. The results showed the ethanol extract of dayak onion (Eleutherine polmifolia (L.) Merr) contained flavonoids, alkaloids, tannins and phenols with the highest levels was by tannins at 7,45%. Dayak onion (Eleutherine polmifolia (L.) Merr) are inhibiting the growth of Salmonella typhi bacteria at a minimum inhibitory concentration (MIC)of 1% with a inhibition zone diameter of 6,24 mm and 0,9% with a inhibition zone diameter of 6,20 mm at Escherichia coli bacteria.Keywords : Eleutherine polmifolia (L.) Merr, Salmonella typhi, Escherichia coli, Agar diffusion, MIC

scholarly journals ANTIBAKTERI SENYAWA FENOLIK DARI ALANG -ALANG (IMPERATA CYLINDRICA)

2021 ◽  
Vol 3 (2) ◽  
pp. 124-126
Author(s):  
Jhon Patar Sinurat ◽  
Suci Wulandari ◽  
Rinaldo Berutu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Phenolic Compounds ◽  
Screening Test ◽  
Inhibition Zone ◽  
Imperata Cylindrica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Agar Diffusion Method ◽  
Escherichia Coli Bacteria

Phytochemical screening test proved that the extract of Reeds (Imperata cylindrica) contained phenolic compounds tested using 5% FeCl3 reagent. Antibacterial test using agar diffusion method against Reeds extract in DMSO solvent. The phenolic compounds obtained from the saputangan leaves Reeds extract were 36.96 grams after undergoing maceration. Reeds extract of phenolic compounds with concentrations (200; 100; 50 and 25 ppm) had strength antibacterial where the average inhibition zone of Staphylococcus aureus bacteria was 10.0 mm and the average inhibition zone was at Escherichia coli bacteria measuring 10.3 mm.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS Leucetta chagosensis DARI PERAIRAN PULAU MANTEHAGE SULAWESI UTARA TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Escherichia coli

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 834
Author(s):  
Eunike Pelealu ◽  
Defny S. Wewengkang ◽  
Surya Sumantri Abdullah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Growth ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Disk Diffusion ◽  
E Coli ◽  
Activity Test ◽  
Escherichia Coli Bacteria ◽  
Metabolite Content

ABSTRACTSponges are one of the biota components that make up coral reefs which are quite widely distributed. The metabolite content in the sponge can ward off and inhibit the pathogenic bacteria that interfere with it. This study aims to determine the activity of inhibiting bacterial growth from the extract and fraction of Leucetta chagosensis sponge against the growth of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. The samples were extracted using the maceration method with 95% ethanol solvent and then fractionated using 3 solvents with different polarity levels, namely methanol, n-hexane and chloroform. Activity test using the disk diffusion agar method of Kirby and Bauer. Only the MeOH fraction was able to inhibit the growth of E. coli bacteria with an average inhibition zone of 6.88 mm. Whereas for S.aureus bacteria extracts and all fractions showed activity to inhibit bacterial growth with an average inhibition zone of EtOH (6.61 mm), CHCI3 (6.68 mm), n-hexane (7.83 mm) and MeOH (8.00 mm), respectively. All activities that are shown are categorized as weak (weak).Keywords: Antibacterial, Leucetta chagosensis, Staphylococcus aureus, Escherichia coli ABSTRAKSpons merupakan salah satu komponen biota penyusun terumbu karang yang penyebarannya cukup luas. Kandungan metabolit yang ada di dalam spons dapat menangkal dan menghambat bakteri patogen pengganggunya.  Penelitian ini bertujuan untuk melihat aktivitas menghambat pertumbuhan bakteri dari ekstrak dan fraksi spons Leucetta chagosensis. terhadap pertumbuhan bakteri Gram positif Staphylococcus aureus dan Gram negatif Escherichia coli. Sampel di ekstraksi menggunakan metode maserasi dengan pelarut etanol 95% lalu di fraksinasi dengan menggunakan 3 pelarut dengan tingkat kepolaran yang berbeda yaitu metanol, n-heksan dan kloroform. Uji aktivitas menggunakan metode disk diffusion agar Kirby dan Bauer.  Hanya fraksi MeOH yang mampu menghambat pertumbuhan bakteri E.coli dengan zona hambat rata-rata 6,88 mm. Sedangkan terhadap bakteri S.aureus ekstrak dan semua fraksi menunjukan aktifitas menghambat pertumbuhan bakteri dengan rata-rata zona hambat masing-masing EtOH (6,61 mm), CHCl3 (6,68 mm), n-Heksan (7,83 mm), dan MeOH (8,00 mm). Semua aktivitas yang ditunjukan dikategorikan lemah (weak).Kata kunci : Antibakteri, Leucetta chagosensis, Staphylococcus aureus,  Escherichia coli

scholarly journals The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 61-67
Author(s):  
Hertina Silaban
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Rare Plants ◽  
E Coli ◽  
Paederia Foetida ◽  
Escherichia Coli Bacteria ◽  
The One

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli. Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals UJI EFEKTIVITAS ANTI BAKTERI EKSTRAK DAUN LEUNCA (SOLANUM NIGRUM) TERHADAP DAYA HAMBAT BAKTERI ESCHERICHIA COLI

2020 ◽  
Vol 3 (1) ◽  
pp. 11-16
Author(s):  
Firdaus Fahdi ◽  
Herviani Sari ◽  
Lisbet Saulina Lubis
Keyword(s):  
Escherichia Coli ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Solanum Nigrum ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Disk Diffusion Method ◽  
Inhibitory Power ◽  
Escherichia Coli Bacteria ◽  
Chemical Contents

Antibacterial is a compound that can inhibit the growth of bacteria that can be used for the treatment of infections in humans. One of the bacteria that can cause interference with the system is the Escherichia coli bacteria, where the Escherichia coli bacteria is widely spread around us. The spread of the Escherichia coli bacteria can occur by direct contact (shaking hands, touching). Then passed on by mouth. Leunca leaves have chemical contents including alkaloids, flavonoids, saponins, tanins. The purpose of this study was to determine whether Leunca's leaf extract (Solanum nigrum) has antibacterial effects against the inhibitory power of Escherichia coli bacteria with a concentration of 50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 250 mg/ml. The method of this study was the sample of Leunca leaf obtained from West Lumban Rau Village, Habinsaran Subdistrict, Toba Samosir Regency. Sample preparation was done by making thick extract with the maceration method. An anti-bacterial effectiveness test is done by the disk diffusion method. The results showed Leunca's leaf extract containing alkaloid compounds, flavonoids, saponins, tannins. As well as having antibacterial effectiveness against the inhibition of Escherichia coli bacteria. Average inhibition zone area concentration of 50 mg/ml = 6.1 mm, concentration of 100 mg/ml = 7.3, concentration of 150 mg/ml = 8.8 mm, concentration of 200 mg/ml = 10.8 mm, concentration of 250 mg/ml = 12.6 mm. Conclusion: Leunca (Solanum nigrum) Leaf Extract has antibacterial effects against the inhibitory power of Escherichia coli bacteria with a strong category.

scholarly journals Uji Efektivitas Ekstrak Akar Tanaman Lauh Putiah (Ficus racemosa L.) Terhadap Bakteri Escherichia coli dan Shigella dysenteriae Penyebab Diare

2019 ◽  
Vol 5 (1) ◽  
pp. 53-63
Author(s):  
Beladiena Citra Siregar ◽  
Welly Darwis ◽  
Mardhatillah Sariyanti
Keyword(s):  
Escherichia Coli ◽  
Medicinal Plants ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Shigella Dysenteriae ◽  
Root Extract ◽  
A Value ◽  
Esherichia Coli ◽  
Ficus Racemosa

ABSTRAKLatar Belakang: Diare merupakan salah satu masalah kesehatan di dunia dan negara berkembang. Mortalitas diare berkisar 17,5-21% dengan ekuivalent 1,5 juta orang setiap tahunnya. Escherichia coli dan Shigella Sp. merupakan patogen utama penyebab diare. Antibioik siprofloksasin yang digunakan untuk mengobati diare memiliki berbagai efek samping. Pemanfaatan tanaman obat merupakan salah satu alternatif untuk mengatasi masalah tersebut. Akar Lauh Putiah merupakan salah satu tanaman obat tradisional yang mempunyai banyak manfaat, salah satunya sebagai antibakteri. Penggunaan akar pada masyarakat Desa Pasar Pino dengan cara meminum air rebusan dari akar Lauh Putiah. Kebiasaan dan pengetahuan tersebut telah secara turun-temurun, namun belum dilatarbelakangi studi ilmiah.Metode: Ekstraksi akar Lauh Putiah dilakukan dengan metode maserasi menggunakan pelarut etanol 96% dan dilarutkan dengan DMSO. Hasil ekstraksi kemudian dilakukan Uji Minimal Inhibitory Concentration (MIC). Setelah dilakukan uji MIC kemudian dilakukan pengujian efektivitas. Kedua uji ini menggunakan metode difusi kertas cakram. Parameter yang digunakan ialah besarnya zona hambat yang terbentuk disekitar kertas cakram, dan kontrol positif yang digunakan adalah larutan antibiotik siprofloksasin 50 µg/ml untuk bakteri Escherichia coli dan bakteri Shigella dysenteriae.Hasil: Hasil pengujian MIC didapatkan bahwa ekstrak akar Lauh Putiah memiliki kemampuan sebagai antibakteri terhadap bakteri Esherichia coli dan Shigella dysentriae. Dari analisis statistik pengujian ANOVA pengaruh ekstrak akar Lauh Putiah menghambat bakteri Escherichia coli dan Shigella sysentriae memiliki nilai Fhitung > Ftabel dengan nilai ? = 0,05 dan kemudian diuji lanjut dengan menggunakan uji Duncan dan didapatkan zona hambat yang efektif konsentrasi yang efektif dalam menghambat pertumbuhan bakteri Escherichia coli adalah konsentrasi 60% (AE3). Pada bakteri Shigella dysentriae didapatkan konsentrasi yang efektif dalam menghambat pertumbuhan bakteri Shigella dysenteriae adalah konsentrasi 87,5% (BE4).Kesimpulan: Ekstrak Akar Tanaman Lauh Putiah (Ficus racemosa L.) memiliki daya hambat terhadap pertumbuhan bakteri Escherichia coli dan Shigella dysentriae. Kata kunci: Escherichia coli, Shigella dysenteriae, Lauh Putiah, Ficus racemosa Linn. ABSTRACTBackground: Diarrhea is one of health problems in the world and developing countries. Diarrhea mortality range is 17.5-21% with an equivalent of 1.5 million people per year. Escherichia coli and Shigella sp. are the main pathogen bacteria that causes diarrhea. Ciprofloxacin is common antibiotic that used to treat diarrhea, but it has various side effects. The use of medicinal plants is an alternative to overcome this problem. Lauh Putiah roots is one of the traditional medicinal plants that has many benefits, one of the benefits is to produce antibacterial compounds. Lauh Putiah roots is used as a medicine for people in Pasar Pino village, it used to heal diarrhea by drinking boiled water from Lauh Putiah roots. These habits have been passed down through generations, but it has not been proofed by scientific studies.Methods: Extraction of Lauh Putiah root was done by maceration method using ethanol 96% and dissolved with DMSO. The extraction results were used to Minimal Inhibitory Concentration (MIC) assay. After MIC assay, effectiveness assay was done. Both of these assay used paper disk diffusion methods. The parameters that used were the amount of inhibition zone formed around the disc paper, and the positive control used ciprofloxacin antibiotic 50 µg/ml for Escherichia coli and Shigella sp.Results: The MIC assay results showed that the roots extract of Lauh Putiah had the ability as an antibacterial against Esherichia coli and Shigella dysentriae. From the statistical analysis of ANOVA test, the effect of Lauh Putiah root extract inhibits Escherichia coli and Shigella sysentriae with a value of Fcount> Ftable with a value of ? = 0.05 and then further tested using Duncan test and found an effective inhibition zone concentration that inhibits growth of Escherichia coli in 60% concentration (AE3) and inhibits growth of Shigella dysentriae  in 87.5% concentration (BE4).Conclusion: Lauh Putiah Root Extract (Ficus racemosa L.) has inhibitory effect on the growth of Escherichia coli and Shigella dysentriae. Keywords:  Escherichia coli, Shigella dysenteriae, Lauh Putiah, Ficus racemosa Linn.

scholarly journals EFFECTS OF ETHANOLIC EXTRACT OF RHINACANTHUS NASUTUS (L) KURZ AS AN ANTIMICROBIAL AGAINST ESCHERICHIA COLI BACTERIA USING IN VITRO METHOD

2019 ◽  
pp. 69-72
Author(s):  
SUNARTI M.BIOMED ◽  
DEBORA PANINSARI
Keyword(s):  
Escherichia Coli ◽  
Treatment Group ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Control Group ◽  
Control Groups ◽  
In Vitro Method ◽  
E Coli ◽  
Escherichia Coli Bacteria

Objective: The objective of this study was to discover of the ethanolic extract of Rhinacanthus nasutus (L) Kurz in inhibiting Escherichia coli bacteria using an in vitro method. Methods: This is an experimental study using a laboratory test with Kirby-Bauer or paper disc method by observing and measuring the inhibition zone of the ethanolic extract of R. nasutus against E. coli bacteria with extract concentrations of 15%, 30%, and 60% consisting of control groups and treatment group. The positive control group used chloramphenicol antibiotics and negative control groups used Aquadest. E. coli was incubated at 37°C for 24 h. Then, the plates were incubated for 24 h at 37°C and the diameter of the inhibition zone was observed until the 3rd day with three repetitions. Results: The results of the study showed that the mean inhibition zone of E. coli bacteria was 10.93 mm, 12.09 mm, and 18.90 mm. The results of the Shapiro–Wilk test were p=0.199. The results of the one-way analysis of variance test were p<0.05 and that of the post hoc test indicated a significant value of p<0.05. Based on the results of the research, there were significant differences in the inhibition zone between the control group and the treatment group at a concentration of 15%, 30%, and 60%. Conclusion: R. nasutus extract was effective to inhibit the growth of E. coli bacteria at concentrations of 15%, 30%, and 60%, so R. nasutus is effective as an antimicrobial.

Pengaruh Konsentrasi Ekstrak Metanol Daun Sambiloto (Andrographis Paniculata Ness) terhadap Sensitivitas Enterobacteriaceae dengan Metode Difusi

2019 ◽  
Vol 2 (1) ◽  
pp. 26
Author(s):  
Parlindungan Nasution ◽  
Sugito Sugito ◽  
Kuswiyanto Kuswiyanto
Keyword(s):  
Escherichia Coli ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Average Value ◽  
Long Time ◽  
Quasi Experimental ◽  
Bacterial Sensitivity ◽  
Escherichia Coli Bacteria ◽  
Spearman Test

Abstract: Sambiloto is plant one of the natural resources that be come important to used as medicine. Sambiloto effcacy had been known since long time ago. Based on it’s pharmacological properties, Sambiloto leaves provide antidiarrheal activity against bacteria that causing diarehea in humans especially Eacherichia coli and Shigella dysentriae bacteria. The main content of Sambiloto leaves are diterpenoid lactones (andrographolide), paniculides, farnesols and flavonoids. The purpose of this research was to study the effect of the methanol extract concentration of Sambiloto leaves toward the sensitivity of Escherichia coli and Shigella dysentriae bacteria by using diffusion method. Research design using quasi experimental research. Sample used were leaf extract of Sambiloto with concentration of 100%, 75%, 50% and 25% with six times repetition. Data was collected and analyzed by using Spearman analysis. Based on the reseach fnding, the measurement of inhibition zone on Escherichia coli bacteria test found that the average value were 14.67 mm at 100% concentration, 13.67 mm at 75% concentration, 12.17 mm at concentration 50% and 10.00 mm at 25% concentration. While Shigella dysentriae bacteria test obtained that average value were 14.33 mm at 100% concentration, 13.33 mm at 75% concentration, 11.17 at concentration 50% and 9.33 mm at 25% concentration. The data were analyzed by Spearman test and the result was (p = 0,000 <0.01). It showed that there was inhibition zone effect of Sambiloto leaves extract on bacterial sensitivity of Escherichia coli and Shigella dysentriae by using diffusion method. It was concluded that the Sambiloto leaves extract is antibacterial.Abstrak: Tanaman Sambiloto merupakan salah satu bahan alam yang semakin banyak peminatnya untuk dijadikan obat. Khasiat Sambiloto sebenarnya sudah dikenal sejak zaman dahulu. Berdasarkan sifat farmakologinya, daun Sambiloto memberikan aktivitas antidiare terhadap bakteri yang menyebabkan diare pada manusia khususnya bakteri Escherichia coli dan Shigella dysentriae. Kandungan utama dari daun Sambiloto adalah diterpenoide lactones (andrographolide), paniculides, farnesols dan flavonoid. Tujuan dari penelitian ini adalah untuk mengkaji pengaruh konsentrasi ekstrak metanol daun sambiloto terhadap sensitivitas bakteri Escherichia coli dan Shigella dysentriae dengan metode difusi. Desain Penelitian ini berbentuk penelitian eksperimental semu. Sampel yang digunakan adalah ekstrak daun sambiloto dengan konsentrasi 100%, 75%, 50% dan 25% dengan pengulangan sebanyak enam kali. Data dikumpulkandan dianalisis dengan analisis Spearman. Berdasarkan hasil penelitian pengukuran zona hambat pada uji bakteri Escherichia coli didapatkan nilai rata-rata 14.67 mm pada konsentrasi 100%, nilai rata-rata 13.67 mm pada konsentrasi 75%, nilai rata-rata 12.17 mm pada konsentrasi 50% dan nilai rata-rata 10.00 mm pada konsentrasi 25%. Sedangkan uji bakteri Shigella dysentriae didapatkan nilai rata-rata 14.33 mm pada konsentrasi 100%, nilai rata-rata 13.33 mm pada konsentrasi 75%, nilai rata-rata 11.17 pada konsentrasi 50% dan nilai rata-rata 9.33 mm pada konsentrasi 25%. Data dianalisis dengan uji Spearman didapatkan hasil (p = 0,000 < 0.01), menunjukan bahwa terdapat pengaruh zona hambat ekstrak daun sambiloto terhadap sensitivitas bakteri Escherichia coli dan Shigella dysentriae dengan menggunakan metode difusi. Dapat disimpulkan bahawa ekstrak daun sambiloto bersifat antibakteri.

scholarly journals IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF 1.5-BIS (3’-ETHOXY-4’-HYDROXYPHENYL)-1-4-PENTADIENE-3-ONE

2018 ◽  
Vol 10 (5) ◽  
pp. 70
Author(s):  
Agus Purwanggana ◽  
Esti Mumpuni ◽  
Esti Mulatsari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Staphylococcus Epidermidis ◽  
In Silico ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Antibacterial Study

Objective: The main objective of this research were screened in vitro and in silico of 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one as potential antibacterial agents.Methods: The in vitro antibacterial study was carried against Staphylococcus aureus, Staphylococcus epidermidis (gram positive) and Escherichia coli, Salmonella thypi (gram negative) using broth dilution method to determine Minimum Inhibitory Concentration (MIC), disc diffusion method to determine the diameter of inhibition zone. In silico antibacterial study was carried using computational software Protein-Ligand ANT System (PLANTS), computational docking was carried using receptor with Protein Data Bank (PDB) file 3MZD. The structures were optimized prior docking using YASARA, and MarvinSketch. The results of antibacterial testing were compared to two positive control drugs i. e amoxicillin and cefadroxil.Results: In vitro evaluation showed that 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has a better antibacterial activity than amoxicillin and cefadroxil with a Minimum Inhibitory Concentration (MIC) of 0.15 ppm and diameter of inhibition zone of 11.27±0.31, 11.35±0.39, 11.25±0.33, and 11.05±0.45 mm in Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Salmonella thypi, respectively. These results in line with in silico evaluation that showed 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one has more negative docking score than amoxicillin, cefadroxil, and cloxacillin acyl as a native ligand on the 3MZD receptor.Conclusion: This results obtained in this research work were 1,5-bis (3'-ethoxy-4'-hydroxyphenyl)-1,4-pentadiene-3-one compound potential as an antibacterial agent. 

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