scholarly journals Investigating the effectiveness of washing cantaloupe melon rind in preventing the transference of surface E. coli into melon flesh

2014 ◽  
Author(s):  
Adam Chan ◽  
BCIT School of Health Sciences, Environmental Health ◽  
Helen Heacock
Keyword(s):  
Escherichia Coli ◽  
Sample Size ◽  
Null Hypothesis ◽  
Foodborne Illness ◽  
P Value ◽  
Indicator Organism ◽  
Chi Square ◽  
E Coli ◽  
Surface Contaminants ◽  
Chi Square Analysis

  Cantaloupe melon was the source of a lethal outbreak of Listeria in 2011. This research investigated whether washing a contaminated cantaloupe rind was sufficient in preventing the transferring of Escherichia coli. Hence, the null hypothesis for this study was that there is no association between washing a contaminated cantaloupe melon and the presence of the contamination in the flesh. In this study, 10 cantaloupes were used to produce a sample size of 20 per washed and unwashed treatments. Each of the samples was transferred to EC broth to determine the presence and absence of Escherichia coli (E. coli), the indicator organism that acted as the “outbreak contaminant.” The results showed 100% of the unwashed melons and 80% of the washed melons to have E. coli transferred into the flesh. A Chi Square analysis produced a p-value of 0.035. The study determined that there was a statistically significant association between washing a melon and the presence of E. coli in the melon flesh. The author recommends washing melon rind as a means to prevent foodborne illness caused by surface contaminants.  

PENGARUH PEMBERIAN PELAYANAN INFORMASI OBAT DAN KONSELING TERHADAP TINGKAT KEPATUHAN MINUM OBAT PADA PASIEN DIABETES MELITUS DENGAN HIPERTENSI DI RUMAH SAKIT DAERAH IDAMAN BANJARBARU

2020 ◽  
Vol 5 (2) ◽  
pp. 240-249
Author(s):  
Satrio Wibowo Rahmatullah ◽  
◽  
Ika Maulida Nurrahma ◽  
Adnan Syahrizal
Keyword(s):  
Drug Information ◽  
Treatment Success ◽  
Information Service ◽  
Regional Hospital ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Quasi Experimental ◽  
High Level ◽  
Chi Square Analysis

Compliance to achieve treatment success can be improved by providing drug information services (PIO) and counseling to improve understanding of treatment instructions. The general objective of this study was to determine the effect of drug information service and counseling on the level of medication adherence in patients with diabetes mellitus (DM) with hypertension in the Banjarbaru Regional Hospital. This study uses a quasi experimental method with a cross sectional approach and takes patient data prospectively. The results of this study indicate that the group given PIO and counseling showed a high level of adherence as many as 20 respondents (100%). Whereas in the group that was not given PIO and the counseling level of adherence was low, there were 4 respondents (20%). Based on the mann-whitney analysis p-value = 0,000 (<α = 0.05) so that there are differences in the group with PIO and counseling with groups without PIO and counseling, while the chi square analysis p-value = 0.004 (<α = 0 , 05) so that PIO and counseling have a significant effect on the level of adherence to taking medication in patients with DM with hypertension at the Banjarbaru Regional Hospital.

scholarly journals ESBL Activity, MDR, and Carbapenem Resistance among Predominant Enterobacterales Isolated in 2019

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 744
Author(s):  
Altaf Bandy ◽  
Bilal Tantry
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
Antimicrobial Resistance ◽  
Carbapenem Resistance ◽  
Resistant Bacteria ◽  
Sociodemographic Characteristics ◽  
Chi Square ◽  
E Coli ◽  
Chi Square Test ◽  
Resistance Rates

Antimicrobial-resistance in Enterobacterales is a serious concern in Saudi Arabia. The present study retrospectively analyzed the antibiograms of Enterobacterales identified from 1 January 2019 to 31 December 2019 from a referral hospital in the Aljouf region of Saudi Arabia. The revised document of the Centers for Disease Control (CDC) CR-2015 and Magiorakos et al.’s document were used to define carbapenem resistance and classify resistant bacteria, respectively. The association of carbapenem resistance, MDR, and ESBL with various sociodemographic characteristics was assessed by the chi-square test and odds ratios. In total, 617 Enterobacterales were identified. The predominant (n = 533 (86.4%)) isolates consisted of 232 (37.6%), 200 (32.4%), and 101 (16.4%) Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis, respectively. In general, 432 (81.0%) and 128 (24.0%) isolates were of MDR and ESBL, respectively. The MDR strains were recovered in higher frequency from intensive care units (OR = 3.24 (1.78–5.91); p < 0.01). E. coli and K. pneumoniae resistance rates to imipenem (2.55 (1.21–5.37); p < 0.01) and meropenem (2.18 (1.01–4.67); p < 0.04), respectively, were significantly higher in winter. The data emphasize that MDR isolates among Enterobacterales are highly prevalent. The studied Enterobacterales exhibited seasonal variation in antimicrobial resistance rates towards carbapenems and ESBL activity.

scholarly journals Characterization of Escherichia coli Strains Derived from Cow Milk of Subclinical and Clinical Cases of Mastitis

2021 ◽  
Vol 11 (2) ◽  
pp. 541
Author(s):  
Katarzyna Grudlewska-Buda ◽  
Krzysztof Skowron ◽  
Ewa Wałecka-Zacharska ◽  
Natalia Wiktorczyk-Kapischke ◽  
Jarosław Bystroń ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacterial Species ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Economic Problem ◽  
Cow Milk ◽  
P Value ◽  
Dairy Herds ◽  
E Coli ◽  
Bonferroni Test

Mastitis is a major economic problem in dairy herds, as it might decrease fertility, and negatively affect milk quality and milk yield. Out of over 150 bacterial species responsible for the udder inflammation, Escherichia coli is one of the most notable. This study aimed to assess antimicrobial susceptibility, resistance to dipping agents and biofilm formation of 150 E. coli strains isolated from milk of cows with subclinical and clinical mastitis. The strains came from three dairy herds located in Northern and Central Poland. The statistical analyses were performed with post-hoc Bonferroni test and chi-square test (including Yates correction). The data with a p value of <0.05 were considered significant. We found that the tested strains were mostly sensitive to antimicrobials and dipping agents. It was shown that 37.33% and 4.67% of strains were resistant and moderately resistant to at least one antimicrobial agent, respectively. No extended-spectrum beta-lactamases (ESBL)-producing E. coli were detected. The majority of strains did not possess the ability to form biofilm or formed a weak biofilm. The strong biofilm formers were found only among strains derived from cows with subclinical mastitis. The lowest bacteria number was noted for subclinical mastitis cows’ strains, after stabilization with iodine (3.77 log CFU × cm−2) and chlorhexidine (3.96 log CFU × cm−2) treatment. In the present study, no statistically significant differences in susceptibility to antibiotics and the ability to form biofilm were found among the strains isolated from cows with subclinical and clinical mastitis. Despite this, infections in dairy herds should be monitored. Limiting the spread of bacteria and characterizing the most common etiological factors would allow proper treatment.

scholarly journals Lactic Acid Bacteria (Lactobacillus bulgaricus and Streptococcus thermophillus) in Yogurt Inhibit the Growth of Escherichia coli, Salmonella typhimurium, and Shigella sp. In Vitro

2021 ◽  
Vol 31 (4) ◽  
pp. 2
Author(s):  
IDSAP Peramiarti
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Test Method ◽  
P Value ◽  
E Coli ◽  
Significant Difference

Diarrhea is defecation with a frequency more often than usual (three times or more) a day (10 mL/kg/day) with a soft or liquid consistency, even in the form of water alone. Pathogenic bacteria, such as Escherichia coli, Salmonella typhimurium, and Shigella sp., play a role in many cases, to which antibiotics are prescribed as the first-line therapy. However, since antibiotic resistance cases are often found, preventive therapies are needed, such as consuming yogurt, which is produced through a fermentation process by lactic acid bacteria (LAB). This research aimed to determine the activity of lactic acid bacteria (Liactobacillus bulgaricus and Streptococcus thermophilus) in yogurt in inhibiting the growth of the pathogenic bacteria E. coli, S. typhimurium, and Shigella sp. The research applied in vitro with the liquid dilution test method and the true experimental design research method with post-test-only and control group design. The design was used to see the inhibitory effect of yogurt LAB on the growth of E. coli, S. typhimurium, and Shigell sp. to compare the effect of several different yogurt concentrations, namely 20%, 40%, 60%, and 80%. The results of the Least Significance Different analysis showed that there was a significant difference between yogurt with a concentration of 0% and that with various concentrations in inhibiting the growth of E. coli, S. typhimurium, and Shigella sp. with a p-value of &lt;0.05. Whereas, there was no significant difference in the various concentrations of yogurt in inhibiting the growth of the three kinds of bacteria with a p-value of &gt; 0.05.<p class="Default" align="center"> </p>

scholarly journals Antibiogram of Diarrhoeagenic Escherichia coli Patients under Five Years Attending Selected Hospitals in Kaduna State, Nigeria

2019 ◽  
pp. 1-11
Author(s):  
R. O. Chioma ◽  
I. Mzungu ◽  
J. B. Orpin
Keyword(s):  
Escherichia Coli ◽  
Nutritional Status ◽  
Good Health ◽  
Latex Agglutination ◽  
Public Health Issue ◽  
Chi Square ◽  
Children Under Five ◽  
E Coli ◽  
Under Five ◽  
Good Nutrition

Escherichia coli infections and poor nutritional status have implications on the growth and development of children under five years, physically, mentally and health wise with consequences such as diarrhoea, stunting, wasting, underweight and often times leading to death, depending on their severity. This study evaluated the antibiogram of Escherichia coli O157 and Verocytotoxigenic Escherichia coli (VTEC)and the nutritional status of diarrhoeic children under five years in Kaduna State, Nigeria, using Conventional isolation methods, latex agglutination tests, VTEC-ELISA tests, Chi-square (SPSS Version 19) and WHO Antro (Version 3.2.2). Purposive sampling was used to select 350 children presenting with diarrhoea in six government hospitals within the three senatorial zones of Kaduna State. The results obtained revealed that 76(21.7%) of the 350 stool samples were positive for E. coli and 28(36.8%) were positive for E. coli O157:H7serotype and 1(1.3%) verocytotoxigenic E. coli (VTEC) serotype. High susceptibility to ciprofloxacin, chloramphenicol and high resistance to sulphamethoxazole, cefotaxime, amoxicillin, gentamicin and tetracycline by the isolates were observed. The study concluded that antibiotics have not been very effective in the treatment of E. coli-related diarrhoea, with VTEC now emerging in this part of the world, making it a serious public health issue. The study therefore recommends the implementation of programmes geared towards good hygiene, good nutrition and good health.

scholarly journals Utilization of MCH handbooks and attitudes of pregnant women regarding pregnancy and childbirth complications

2019 ◽  
Vol 27 (2) ◽  
pp. 71
Author(s):  
Dian Irawati ◽  
Agustin Dwi Syalfina
Keyword(s):  
Pregnant Women ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Live Births ◽  
Pregnancy And Childbirth ◽  
Independent Variable ◽  
The Right ◽  
Chi Square Analysis ◽  
The Relationship

Objectives: Maternal Mortality Rate (MMR) is closely related to maternal care. MMR in Indonesia based on the 2015 IDHS is 359 per 100,000 live births. By increasing the utilization of MCH handbooks, MMR would be decline. Therefore, this research investigated the relationship between of the utilization of MCH handbooks and attitudes pregnant women regarding pregnancy and childbirth complications.Materials and Methods: We conducted a cross sectional research with 54 pregnant women during March - June 2018 at the Sooko Health Center, Mojokerto. The independent variable was the utilization of MCH handbooks and the dependent variable was the attitude of pregnan women regarding pregnancy and childbirth complications. Data analysis included descriptive and bivariate analysis.Results: The results showed that 79.6% of respondents used the MCH handbook well. The results of the chi square analysis test showed a p value of 0.027 (<0.05).Conclusion: MCH handbook utilization effected the attitude of pregnant women regarding the complication of pregnancy and childbirth. Pregnant women who read and utilize MCH handbook would be have better alertness about the risk of complication so they would make the right decision for their pregnancy.

Effect of Sampling Plans on the Risk of Escherichia coli O157 Illness

2015 ◽  
Vol 78 (7) ◽  
pp. 1370-1374
Author(s):  
ANDREAS KIERMEIER ◽  
JOHN SUMNER ◽  
IAN JENSON
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Sample Size ◽  
Health Effect ◽  
The United States ◽  
Escherichia Coli O157 ◽  
Sampling Protocol ◽  
Sampling Plans ◽  
E Coli ◽  
Sample Amount

Australia exports about 150,000 to 200,000 tons of manufacturing beef to the United States annually. Each lot is tested for Escherichia coli O157 using the N-60 sampling protocol, where 60 small pieces of surface meat from each lot of production are tested. A risk assessment of E. coli O157 illness from the consumption of hamburgers made from Australian manufacturing meat formed the basis to evaluate the effect of sample size and amount on the number of illnesses predicted. The sampling plans evaluated included no sampling (resulting in an estimated 55.2 illnesses per annum), the current N-60 plan (50.2 illnesses), N-90 (49.6 illnesses), N-120 (48.4 illnesses), and a more stringent N-60 sampling plan taking five 25-g samples from each of 12 cartons (47.4 illnesses per annum). While sampling may detect some highly contaminated lots, it does not guarantee that all such lots are removed from commerce. It is concluded that increasing the sample size or sample amount from the current N-60 plan would have a very small public health effect.

Validity of Pediatric Index of Mortality 2 score as an Outcome Predictor in Pediatric ICU of a Public Sector Tertiary Care Hospital in Pakistan

2021 ◽  
Author(s):  
Muhammad Bilal Mazhar ◽  
Muhammad Haroon Hamid
Keyword(s):  
Intensive Care ◽  
Population Characteristics ◽  
P Value ◽  
Care Hospital ◽  
Outcome Predictor ◽  
Goodness Of Fit Test ◽  
Chi Square ◽  
Common Diagnosis ◽  
Chi Square Analysis ◽  
Pediatric Index Of Mortality

AbstractPediatric Index of Mortality 2 (PIM-2) is one of the leading mortality scores used in intensive care units all around the world. We assessed its validity as an outcome predictor in a pediatric intensive care unit (PICU) of Mayo Hospital/King Edward Medical University Lahore, Pakistan. We enrolled 154 consecutive admissions, aged 1 month to 13 years, requiring intensive care from January to June of 2019. Patient demographics along with PIM-2 data were collected; PIM-2 score and mortality risk was calculated; and the outcome recorded as death or survival. The median age at admission was 0.50 years (interquartile range [IQR]: 0.24–1.78) and the median weight was 5.0 kg (IQR: 3.08–10.0) with females constituting 54%; malnutrition was also common (66%). Observed mortality was 29.9% (46 out of 154) and expected mortality (cut-off ≥ 99.8%) was 27.9% with a standardized mortality ratio of 1.07 (95% confidence interval [CI]: 0.79–1.41). Sepsis was the most common diagnosis at admission (27.9%) with the highest mortality (52.2%). Chi-square analysis revealed a sensitivity of 54.3% and a specificity of 83.3% (p-value 0.00). PIM-2 score showed acceptable discrimination between survivors and nonsurvivors with an area under the receiver operating characteristic curve of 0.75 (95% CI: 0.67–0.84) (p-value = 0.00); however, poor calibration according to Hosmer–Lemeshow goodness of fit test (Chi-square = 15.80, df = 7, and p-value of 0.027 [< 0.1]), thus requiring recalibration according to local population characteristics.

Dihydropyrimidine dehydrogenase deficiency in patients treated with 5FU or capecitabine based regimens: A single center experience from South India.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e15517-e15517
Author(s):  
Keechilat Pavithran ◽  
Prasanth Ariyannur ◽  
Hridya Jayamohanan ◽  
Arun Philip ◽  
Wesley Mannirathil Jose ◽  
...  
Keyword(s):  
Dihydropyrimidine Dehydrogenase ◽  
Asian Population ◽  
Common Variant ◽  
P Value ◽  
Toxicity Profile ◽  
South Asian Population ◽  
Chi Square ◽  
Single Center Experience ◽  
Individualized Approach ◽  
Chi Square Analysis

e15517 Background: DPYD deficiency is present in 3-5% of patients. The risk of treatment-related death in DPYD mutation carriers who receive fluoropyrimidine chemotherapy has been estimated at 3-10%. There is growing data in support of widespread screening for DPYD deficiency prior to fluoropyrimidine chemotherapy. The purpose of our study was to identify the prevalence of DPYD deficiency among patients receiving 5-fluorouracil (5FU) or capecitabine based combination chemotherapy, and to analyse the toxicity profile among the DPYD deficient group. Methods: The study involved patients who have received 5FU and capecitabine based chemotherapy for the treatment for gastrointestinal, breast and head and neck cancers between 2019-2020. Four DPYD variants were checked using real-time PCR based Genotyping assay. Three common DPYD variants recommended by the CPIC guidelines (IVS14+1 G>A (*2A), c.1679T>G (*13), c.2846A>T) and a common variant in Asian population (c.496A>G), based on previous studies, was included in our analysis. These variants were assessed prior to the initiation of the chemotherapy and dose was modified based on the activity score and the toxicity profile was assessed. Descriptive statistics was performed using SPSS version 20. Results: 375 patients were included in this analysis. The median age of the cohort was 61years (21-84 years). 51.3% were males and 49.7% females. Among the 375 patients, 47 patients had DPYD mutation (12.5%). The median age of the DPYD mutated patients were 68 years; 29 (61.7%) males and 18 (38.2%) females. 32 (68.8%) had deleterious mutation in DPYD variant c.496A>G (rs2297595) and 15 (31.9%) showed mutation IVS14+1 G>A (rs3918290). 35 out 47 patients had grade II-III toxicity even after dose reduction during the first cycle of chemotherapy. The commonly seen adverse events were hand and foot syndrome in 18 (38.3%), mucositis in 7 (14.9%), diarrrhoea in 15 (31.9%) and neutropenia in 25 (31.9%) patients. Four patients had febrile neutropenia. One patient experience myocardial infarction. There was no mortality. Chi square analysis showed DPYD mutation had significant association with presence of severe adverse reaction (74.5%, p-value. 0.002). Conclusions: Prevalence of DPYD mutation in our patients was 12.5%. They experienced more toxicities while receiving 5-FU/Capecitabine even after dose modification. c.496A>G (rs2297595) was the most common variant seen in our patients. Screening of patients for DPYD mutations prior to administration of 5-FU/capecitabine using pharmacogenetic methods may help identify those patients who are at risk for adverse effects, allowing a more individualized approach to their chemotherapy management. c.496A>G (rs2297595) variant should also be in included routinely in DPYD screening among South Asian population.

Number of Bleeding, Placenta Remaining, and Episiotomy Actions on the Incidence of Puerperal Sepsis

2021 ◽  
Vol 2 (2) ◽  
pp. 134-138
Author(s):  
Sheryl Putri Asri ◽  
Soetimah
Keyword(s):  
Sampling Method ◽  
Analytical Description ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Puerperal Sepsis ◽  
Birth Complications ◽  
Chi Square Analysis ◽  
Postpartum Mothers

It is estimated that 60% of maternal mortality resulting from gestation happened right after giving birth, and 50% of puerperium deaths happened in the first 24 hours. This research aims to know the relation between the amount of bleeding, remaining placenta, and the act of episiotomy to puerperal sepsis cases. This research uses analytical description methods with a cross-sectional approach. The population of this research was the postpartum mothers in puerperal sepsis cases in October – December 2017 at RSAB Harapan Kita Jakarta Barat counted 85 persons. The sampling method used in this research is an accidental sampling method with 32 respondents. The Instruments of this research are medical records and questionnaires with the chi-square analysis method. This research data analysis has the quality of univariate and bivariate, which means knowing about the relation between the amount of bleeding, remaining placenta, and the act of episiotomy to puerperal sepsis cases. This research shows a relation between the amount of bleeding with a p-value (0,035) and OR (7,200). There is a relation between remained placenta variable with a p-value (0,035) and OR (7,200). There is no relation in the act of episiotomy variable with p-value (0,142) and OR (3,500) to puerperal sepsis cases. The researcher suggests RSAB Harapan Kita increase the quality of their health services, mainly socialize information and give education for maternity women about various birth complications, such as bleeding and remaining placenta and sepsis puerperalis's risks.

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