Evaluation of the efficacy of systemic danofloxacin in the treatment of induced acuteEscherichia colibovine mastitis

2008 ◽  
Vol 75 (3) ◽  
pp. 310-318 ◽  
Author(s):  
Bernard Poutrel ◽  
Michael R Stegemann ◽  
Olivier Roy ◽  
Francis Pothier ◽  
Nicola Tilt ◽  
...  
Keyword(s):  
Milk Production ◽  
Pathogenic Bacteria ◽  
Bovine Mastitis ◽  
Clinical Signs ◽  
Saline Control ◽  
Intravenous Route ◽  
Post Inoculation ◽  
General Behaviour ◽  
Cell Counts ◽  
Clinical Scores

The objective was to evaluate the efficacy of a single dose of danofloxacin (6 mg/kg bodyweight) given by the intravenous route for the treatment of acute bovine mastitis induced by intra-cisternal infusion of anEscherichia colistrain (26 cfu into one rear quarter of each cow). Twenty-three Prim'Holstein lactating cows were inoculated. To be challenged, the mammary glands had to be productive, free of pathogenic bacteria, and with somatic cell counts (SCC) of <200 000 cells/ml. The cows were treated on an individual basis when predetermined criteria involving both systemic and local clinical signs were satisfied. Allocation to treatment, danofloxacin or negative saline control, was performed according to a randomized treatment allocation plan. Monitoring during a 21-d period after inoculation included individual clinical examination, bacteriological examination and determination of SCC.Esch. coliwas isolated from the milk of all inoculated quarters at the first milking post-inoculation and, together with reference to the clinical scores; the challenge was considered to be successful in 20 of the 23 cows. On study day 7 bacteriological cure rates with danofloxacin and saline control were 89% (8/9) and 44% (4/9) respectively. On days 14 and 21 all milk samples that could be collected were negative forEsch. coliin both groups of animals. Beneficial statistically significant differences were found at the end of the observation period (days 19–21 post treatment) between cows treated with danofloxacin and saline for SCC (P=0·0091) and earlier in the study for milk production (P=0·0003) and udder inflammation (P=0·004). Obvious beneficial trends were recorded in the danofloxacin group for rectal temperature, milk quality, general behaviour and appetite. Danofloxacin-treated cows showed statistically significant lower local clinical scores and a more rapid return to pre-inoculation values. It was concluded that systemically administered danofloxacin is effective in terms of bacteriological results, milk production and both systemic and local signs when used in the treatment of induced acuteEsch. colimastitis. Danofloxacin hastens recovery and return to productivity compared with potential self cure.

Histological examination of non-lactating bovine udders inoculated with Lactobacillus perolens CRL 1724

2012 ◽  
Vol 80 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Ignacio D Frola ◽  
Matías S Pellegrino ◽  
Gabriel Magnano ◽  
José A Giraudo ◽  
María C Espeche ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Histological Examination ◽  
Inflammatory Reaction ◽  
Bovine Mastitis ◽  
Clinical Signs ◽  
Structural Aspect ◽  
Post Inoculation ◽  
Lactating Dairy Cows ◽  
Preliminary Study ◽  
Cell Alterations

The effect of intramammary inoculation of Lactobacillus perolens CRL 1724 on bovine udders at drying off was evaluated through histological examination of the canal and cistern tissues. The persistence of the strain in the udder 7 d post inoculation was also determined. Lb. perolens CRL 1724 was recovered from all mammary quarters and no clinical signs or teat damage were observed after inoculation of 106 cfu/ml. The udders showed a normal structural aspect and there were no modifications of the milk appearance. Lb. perolens CRL 1724 cells were evidenced on the surface of the epithelial cells of the cistern without causing any morphological modifications or cell alterations. Lb. perolens CRL 1724 produces a mild inflammatory reaction, characterized by recruitment of neutrophils to the epithelial zone and a slight hyperaemia into blood vessels. This preliminary study provides important information for further studies directed towards the inclusion of Lb. perolens CRL 1724 in the design of probiotic products for preventing bovine mastitis in non-lactating dairy cows.

72 A One-health Perspective on S. aureus Induced Mastitis

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 39-40
Author(s):  
Xin Zhao ◽  
Eveline M Ibeagha-Awemu ◽  
Pierre Lacasse
Keyword(s):  
Public Health ◽  
Pathogenic Bacteria ◽  
One Health ◽  
Methicillin Resistance ◽  
Bovine Mastitis ◽  
Indirect Evidence ◽  
Health Concern ◽  
Effective Control ◽  
Methicillin Resistant ◽  
Cell Counts

Abstract Bovine mastitis, commonly caused by bacteria, is one of the most devastating diseases for the dairy industry worldwide, with high economic, social, and public health impacts. Among the pathogenic bacteria, Staphylococcus aureus is one of the leading pathogens in most countries. Success rate of antibiotic treatment is low such that effective control of S. aureus induced mastitis is currently only possible through prevention of new infections and culling of infected animals. The infection is usually subclinical, causing elevated somatic cell counts but no detectable changes in milk or the udder. S. aureus persists in mammary glands, teat canals, and teat lesions of infected cows and is transmitted to other cows during milking. The direct evidence of cross-species transmission of S. aureus is still scarce. Nevertheless, accumulating indirect evidence supports the zoonotic potential for S. aureus from bovine mastitis to humans. In addition to direct contact, aerosol exposures may also be an important mechanism for the transmission. While animal-adapted livestock-associated methicillin-resistant S. aureus has been known for many years, most S. aureus isolated from mastitic milk in Canada are methicillin-sensitive. S. aureus induced mastitis is a common reason for therapeutic and/or prophylactic use of antibiotics on dairy farms. Occurrence of multidrug resistant, especially methicillin-resistant, S. aureus has been a major public health concern. In this talk, we will address the pathogenesis and strain spectrum of S. aureus induced bovine mastitis, review existing evidence for inter-species transmission, and discuss possible transmission of methicillin-resistance determinants. This talk aims to emphasize the need for the one-health approach for prevention and treatment of S. aureus induced mastitis, in order to provide safe and nutritious milk and milk products to consumers.

Effects of intramammary inoculation ofLactobacillus perolensCRL1724 in lactating cows’ udders

2011 ◽  
Vol 79 (1) ◽  
pp. 84-92 ◽  
Author(s):  
Ignacio D Frola ◽  
Matías S Pellegrino ◽  
María C Espeche ◽  
José A Giraudo ◽  
María EF Nader-Macias ◽  
...  
Keyword(s):  
Bovine Mastitis ◽  
Clinical Signs ◽  
Probiotic Properties ◽  
Lactating Cows ◽  
Cell Counts ◽  
Major Pathogens ◽  
Adhesion Index ◽  
Teat Canal

Bovine mastitis is the most important infectious disease on dairy farms. Conventional antibiotic therapy is often unsatisfactory and alternative treatments are continually under investigation.Lactobacillus (Lb.) perolensCRL 1724 andLactobacillus plantarumCRL 1716 were previously isolated from milk of dairy cows and selected according to their potential probiotic properties. In the present work the in-vitro capacity ofLactobacillusstrains to adhere to bovine teat canal epithelial cells (BTCEC) and to inhibit and co-aggregate 14 mastitis-causing pathogens (MCPs) was investigated. The effect ofLb. perolensCRL 1724 after intramammary inoculation in lactating cows was evaluated through determination of clinical signs of mastitis, milk appearance, somatic cell counts andLb. perolensCRL 1724 recovery from milk.Lb. perolensCRL 1724 was able to inhibit 12 of 14 MCPs (85·7%) in vitro, especially those considered to be major pathogens. In addition,Lb. perolensCRL 1724 co-aggregated with all of them.Lb. plantarumCRL 1716 was able to inhibit 7 of 14 MCPs (50%) in vitro and showed co-aggregation ability similar toLb. perolensCRL 1724.Lb. perolensCRL 1724 showed a higher efficacy of adhesion to BTCEC (values of percentage of adhesion and adhesion index of 75% and 14·4, respectively) thanLb. plantarumCRL 1716 (37% and 7·4, respectively).Lb. perolensCRL 1724 was recovered from all mammary quarters and no clinical signs or teat damage were observed after the inoculation of 106cfu/ml. The udders presented a normal aspect and there were no changes in the appearance of the milk. The results obtained will serve as the basis for further trials to evaluate the potential ofLb. perolensCRL 1724 to be included in a non-antibiotic formulation for the prevention of bovine mastitis.

scholarly journals Ultrasensitive haptoglobin biomarker detection based on amplified chemiluminescence of magnetite nanoparticles

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Narsingh R. Nirala ◽  
Yifat Harel ◽  
Jean-Paul Lellouche ◽  
Giorgi Shtenberg
Keyword(s):  
Magnetite Nanoparticles ◽  
Pathogenic Bacteria ◽  
Limit Of Detection ◽  
Early Stage ◽  
Specific Interaction ◽  
Bovine Mastitis ◽  
Cost Effective ◽  
Quantitative Detection ◽  
Label Free ◽  
Cell Counts

Abstract Background Haptoglobin is an acute-phase protein used as predicting diagnostic biomarker both in humans (i.e., diabetes, ovarian cancer, some neurological and cardiovascular disorders) and in animals (e.g., bovine mastitis). The latter is a frequent disease of dairy industry with staggering economical losses upon decreased milk production and increased health care costs. Early stage diagnosis of the associated diseases or inflammation onset is almost impossible by conventional analytical manners. Results The present study demonstrates a simple, rapid, and cost-effective label-free chemiluminescence bioassay based on magnetite nanoparticles (MNPs) for sensitive detection of haptoglobin by employing the specific interaction of hemoglobin-modified MNPs. The resulting haptoglobin-hemoglobin complex inhibits the peroxidase-like activity of luminol/H2O2-hemoglobin-MNPs sensing scheme and reduces the chemiluminescence intensities correspondingly to the innate haptoglobin concentrations. Quantitative detection of bovine haptoglobin was obtained within the range of 1 pg mL−1 to 1 µg mL−1, while presenting 0.89 pg mL−1 limit of detection. Moreover, the influence of causative pathogenic bacteria (i.e., Streptococcus dysgalactiae and Escherichia coli) and somatic cell counts (depicting healthy, sub-clinical and clinical mastitis) on the emitted chemiluminescence radiation were established. The presented bioassay quantitative performances correspond with a standardized assay kit in differentiating dissimilar milk qualities. Conclusions Overall, the main advantage of the presented sensing concept is the ability to detect haptoglobin, at clinically relevant concentrations within real milk samples for early bio-diagnostic detection of mastitis and hence adjusting the precise treatment, potentially initiating a positive influence on animals’ individual health and hence on dairy farms economy.

Intramammary infusion of a live culture ofLactococcus lactisfor treatment of bovine mastitis: comparison with antibiotic treatment in field trials

2008 ◽  
Vol 75 (3) ◽  
pp. 365-373 ◽  
Author(s):  
Katja Klostermann ◽  
Fiona Crispie ◽  
James Flynn ◽  
R Paul Ross ◽  
Colin Hill ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Bovine Mastitis ◽  
Clinical Signs ◽  
Experimental Period ◽  
Treated Group ◽  
Field Trials ◽  
Clinical Mastitis ◽  
Post Treatment ◽  
Cell Counts ◽  
Live Culture

A treatment containing a live food-grade organism,Lactococcus lactisDPC3147, was compared with conventional antibiotic therapy for its potential to treat bovine chronic subclinical or clinical mastitis in two separate field trials. Effects on disease symptoms and bacteriology were monitored in response to infusion with the culture in each trial. In the first trial, the live culture treatment was compared with an intramammary antibiotic (n=11 quarters for each treatment). Results from this small trial demonstrated that the live culture had potential to be as effective at eliminating chronic subclinical infections as an antibiotic treatment. By day 12, 7 of the 11 quarters treated with the live culture were pathogen-free compared with 5 of the 11 antibiotic-treated infected quarters. Somatic cell counts (SCC) remained relatively unchanged regardless of treatment: average log SCC pre- and post-treatment in the lactococci-treated group were 6·33±0·41 (day 0) and 6·27±0·43 cells/ml (day 12) and average log SCC pre- and post-treatment in the antibiotic-treated group were 6·34±0·37 and 6·22±0·46 cells/ml on day 0 and on day 12, respectively. In the second trial, the live culture was compared with an intramammary antibiotic for the treatment of naturally occurring clinical mastitis cases (n=25 quarters for each treatment). Following a 14-d experimental period, similar bacteriological responses were observed in 7 out of 25 live culture treated quarters and 9 out of 25 antibiotic-treated quarters. Additionally, 15 of 25 cases treated with the culture and 18 of 25 cases treated with the antibiotic did not exhibit clinical signs of the disease following treatment. The results of these trials suggest that live culture treatment withLc. lactisDPC3147 may be as efficacious as common antibiotic treatments in some instances.

Treatment of acute Escherichia coli mastitis in cows with enrofloxacin: effect on clinical signs and chemiluminescence of circulating neutrophils

2000 ◽  
Vol 67 (4) ◽  
pp. 485-502 ◽  
Author(s):  
DAGMAR HOEBEN ◽  
ERICA MONFARDINI ◽  
CHRISTIAN BURVENICH ◽  
JÖRN HAMANN
Keyword(s):  
Escherichia Coli ◽  
Milk Production ◽  
Clinical Signs ◽  
Milk Composition ◽  
Polymorphonuclear Leucocytes ◽  
Beneficial Effects ◽  
Cell Counts ◽  
Effect Of Treatment ◽  
Severity Of The Disease ◽  
Loss Of Appetite

We have studied the effect of treatment with enrofloxacin on local and general clinical signs and chemiluminescence of circulating polymorphonuclear leucocytes during experimentally induced Escherichia coli mastitis in cows immediately after parturition. Twelve cows were infected with 104 cfu Esch. coli P4[ratio ]O32 into both left quarters. Six cows received an intravenous injection of 5 mg enrofloxacin/kg at 10 h after infection and a second enrofloxacin treatment administered subcutaneously at 30 h post infection. The other six cows were controls that received no treatment. General clinical signs (fever, tachycardia, loss of appetite, reduced rumen motility and depression) were similar in both groups. Local clinical signs, such as swelling, pain and firmness of the inflamed mammary quarters, were less severe in the treated cows. We saw no difference in the appearance of the milk[ratio ]flecks and watery or purulent milk were observed in both groups. The beneficial effects of treatment with enrofloxacin were mainly on milk production and composition. The decline in milk production and the changes in milk concentrations of lactose, Na+ and bovine serum albumin were less pronounced in the treated cows. Treatment with enrofloxacin accelerated the clearance of bacteria from the infected quarters, but had no effect on the chemiluminescence response of isolated polymorphonuclear leucocytes. The changes in the number of circulating leucocytes and the appearance of immature neutrophils in the circulation of the treated cows indicated possible beneficial effects on migration of neutrophils into the inflamed glands. Higher milk somatic cell counts in the treated cows supported this hypothesis. The results of this study indicated that treating cows that have been experimentally infected with Esch. coli mastitis after parturition with enrofloxacin reduced the severity of the disease, especially the decline in milk production and the changes in milk composition.

Relationship between the level of N-acetyl-β-D-glucosaminidase (NAGase) in bovine milk and the presence of mastitis pathogens

1984 ◽  
Vol 51 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Barry J. Kitchen ◽  
W. Seng Kwee ◽  
Gordon Middleton ◽  
R. Jeffrey Andrews
Keyword(s):  
Tissue Damage ◽  
Pathogenic Bacteria ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Marker Enzyme ◽  
Coagulase Negative Staphylococci ◽  
Cell Counts ◽  
Major Pathogens ◽  
Mastitis Pathogens ◽  
Damage Marker

SummaryChanges in the level of the tissue damage marker enzyme, N-acetyl-β-D-glucosaminidase (NAGase) in quarter fore milks were found to be related to the presence and types of pathogenic bacteria present and to somatic cell counts (SCC). Minor pathogens (coagulase-negative staphylococci, Corynebacterium bovis) elicited a mild SCC increase (from a mean of 243 × 103/ml in healthy quarters to 504 × 103/ml in infected quarters) with marginal tissue damage (mean NAGase activity increased from 21 in healthy quarters to 28 in infected quarters). Major pathogens (i.e. Staphylococcus aureus, Streptococcus agalactiae, Str. dysgalactiae and Str. uberis) caused more severe tissue damage (mean NAGase of 48) and SCC increases (mean, 2803 × 103/ml). The NAGase test could also be used effectively on composite milk samples where regular monthly NAGase analysis was able to identify correctly 74% of animals having infected quarters. The possibility of combining SCC and NAGase data in order to give a more definite diagnosis of bovine mastitis is discussed.

Comparison of milk production, mastitis, lameness and days to conception of Brown Swiss, Holstein and Holstein cross Brown Swiss dairy cattle

2006 ◽  
Vol 2006 ◽  
pp. 86-86
Author(s):  
G Wellwood ◽  
J K Margerison
Keyword(s):  
Dairy Cattle ◽  
Somatic Cell ◽  
Milk Production ◽  
Milk Yield ◽  
Complex Disease ◽  
Economic Loss ◽  
Brown Swiss ◽  
Somatic Cell Counts ◽  
Cell Counts ◽  
Holstein Friesian

Mastitis is a complex disease causing inflammation of the udder, which has been estimated to cost the dairy farmer between £40-£117/cow per year (Stott et al., 2002). Economic loss occurs as a result of discarded milk, reduced milk yield and milk quality, increased vet costs and an increase in replacement costs. The objective of this study was to examine the effect of breed on the incidence of mastitis and somatic cell counts and milk production capabilities of Holstein Friesian, Brown Swiss and Brown Swiss crossbred cows.

scholarly journals Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia)

2016 ◽  
Vol 37 (4) ◽  
pp. 1919
Author(s):  
Átilla Holanda de Albuquerque ◽  
Régis Siqueira de Castro Teixeira ◽  
Débora Nishi Machado ◽  
Elisângela De Souza Lopes ◽  
Ruben Horn Vasconcelos ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Clinical Signs ◽  
Columba Livia ◽  
Poultry Feed ◽  
Economic Losses ◽  
Control Group ◽  
Broiler Chicks ◽  
Post Inoculation ◽  
Microbiological Analysis ◽  
Fecal Samples

Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results from cloacal swabs demonstrated bacterial elimination in 68.8% and 53.1% of G2 and G1 birds, respectively at 1 dpi. Additionally, fecal samples had elevated bacterial shedding in all four periods of observation , with a higher excretion at 4 dpi (62.5%) for both groups. Among G2 birds, 74.2% were positive for the pathogen in the intestine; G1 birds presented the lowest rate of lung infection (29%), and both groups had more than 50% positivity for liver and caeca. The results revealed that infected chicks with a Salmonella Typhimurium strains isolated from pigeons may host the pathogen in several organs, and simultaneously present diarrheic disorders with significant levels of bacterial excretion in feces.

scholarly journals A Real-Time Thermal Sensor System for Quantifying the Inhibitory Effect of Antimicrobial Peptides on Bacterial Adhesion and Biofilm Formation

Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2771
Author(s):  
Tobias Wieland ◽  
Julia Assmann ◽  
Astrid Bethe ◽  
Christian Fidelak ◽  
Helena Gmoser ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Real Time ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Bovine Mastitis ◽  
Inhibitory Effect ◽  
Thermal Sensor ◽  
Bacterial Cells ◽  
Static Conditions

The increasing rate of antimicrobial resistance (AMR) in pathogenic bacteria is a global threat to human and veterinary medicine. Beyond antibiotics, antimicrobial peptides (AMPs) might be an alternative to inhibit the growth of bacteria, including AMR pathogens, on different surfaces. Biofilm formation, which starts out as bacterial adhesion, poses additional challenges for antibiotics targeting bacterial cells. The objective of this study was to establish a real-time method for the monitoring of the inhibition of (a) bacterial adhesion to a defined substrate and (b) biofilm formation by AMPs using an innovative thermal sensor. We provide evidence that the thermal sensor enables continuous monitoring of the effect of two potent AMPs, protamine and OH-CATH-30, on surface colonization of bovine mastitis-associated Escherichia (E.) coli and Staphylococcus (S.) aureus. The bacteria were grown under static conditions on the surface of the sensor membrane, on which temperature oscillations generated by a heater structure were detected by an amorphous germanium thermistor. Bacterial adhesion, which was confirmed by white light interferometry, caused a detectable amplitude change and phase shift. To our knowledge, the thermal measurement system has never been used to assess the effect of AMPs on bacterial adhesion in real time before. The system could be used to screen and evaluate bacterial adhesion inhibition of both known and novel AMPs.

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