Rhamnus alaternus aqueous extract enhances the capacity of system redox defence and protects hepatocytes against aluminum chloride toxicity in rats

Abstract Background This study was designed to evaluate the protective effects of a Rhamnus alaternus aqueous extract (RAAE) on aluminum chloride-induced hepatotoxicity in rats. A preliminary phytochemical study and antioxidant activity tests of the extract were performed. Methods A preliminary phytochemical study and antioxidant activity tests of the extract were performed. For the in vivo study, twenty-four male rats were divided into four groups. The control group (C); the RAAE group treated with 250 mg/kg b.w RAAE; the AlCl3 group, which received 50 mg/kg b.w AlCl3; and the AlCl3/RAAE group that was treated with AlCl3 plus RAAE. Results The RAAE contains several phenolic compounds. This plant extract showed a high radical scavenging effect and high antioxidant activity. Administration of AlCl3 resulted in a significant increase in the activities of aspartate aminotransferase and alanine aminotransferase (AST, ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) and significant decreases in the plasma concentrations of total proteins and albumin. Moreover, AlCl3 induced a hepatic pro-oxidant effect leading to an increase in malonaldehyde (MDA) and carbonyl protein contents, the depletion of the content of reduced glutathione (GSH) and a decrease in the antioxidant enzymatic activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). However, RAAE supplementation with AlCl3 treatment significantly decreased the levels of MDA and carbonyl proteins and markedly restored the activities of the antioxidant enzymes. These results are supported by the improvement in liver tissue restoration. Conclusions The Rhamnus alaternus aqueous extract was shown to have effective antioxidant activity owing to its phenolic compounds protecting against AlCl3-induced liver oxidative damage.

Download Full-text

Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats

BioMed Research International ◽

10.1155/2021/7202447 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Mohammed A. Alsieni ◽

Haddad A. El Rabey ◽

Abdulbasit I. Al-Sieni ◽

Madeha N. Al-Seeni

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Density Lipoprotein ◽

Negative Control ◽

Seed Extract ◽

Male Rats ◽

Antidiabetic Activity ◽

Control Group ◽

Albino Rats ◽

Leaf Aqueous Extract

This study is aimed at comparing the antidiabetic and antioxidant potential of fenugreek and buckthorn which are commonly used in modulating diabetes in the Middle East. In this study, the antioxidant and antidiabetic activity of the aqueous extracts of the leaf and seed of fenugreek and buckthorn was tested in streptozotocin-induced diabetic male rats fed with a fat-rich diet for 8 weeks. Thirty-six male albino rats were divided into 6 groups ( n = 6 ); the 1st group was the negative control. Diabetes was induced in the other 30 rats using streptozotocin, which were then divided into 5 groups; the 2nd was the untreated positive diabetic group, the 3rd was treated with fenugreek leaf aqueous extract, the 4th was treated with the fenugreek seed aqueous extract, the 5th was treated with buckthorn leaf aqueous extract, and the 6th was treated with buckthorn seed aqueous extract. The positive control group showed an increase in blood sugar, glycated hemoglobin, liver function enzymes, lactate dehydrogenase, kidney indices, total cholesterol, triglycerides, low- and very-low-density lipoprotein, immunoglobulins, and lipid peroxidation and a decrease in high-density lipoprotein, albumin, and antioxidant activity. The histology of the liver and testes showed severe histopathological alterations. Rats of groups 4-6 that were treated with the aqueous extract of the leaf and seed extract of fenugreek and buckthorn showed improvement of all biochemical and histopathological parameters. The seed extract of fenugreek and buckthorn showed more antioxidant activity than their leaves.

Download Full-text

Effect of Opuntia dejecta (Salm-Dyck) flowers in liver of fructose-fed rats: Biochemicals, enzymatic and histological studies

Human & Experimental Toxicology ◽

10.1177/09603271211013448 ◽

2021 ◽

pp. 096032712110134

Author(s):

O Zouaoui ◽

K Adouni ◽

A Jelled ◽

A Thouri ◽

A Ben Chrifa ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Body Weight ◽

Diabetes Type 2 ◽

Male Rats ◽

Control Group ◽

Standard Drug ◽

High Fructose ◽

Group A ◽

Histological Architecture

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.

Download Full-text

Subchronic toxicity of aqueous extract of Alstonia boonei de wild. (apocynaceae) stem bark in normal rats

International Journal of Pharmacology and Toxicology ◽

10.14419/ijpt.v3i1.4625 ◽

2015 ◽

Vol 3 (1) ◽

pp. 5 ◽

Cited By ~ 2

Author(s):

Barnabé Lucien Nkono Ya Nkono ◽

Selestin Dongmo Sokeng ◽

Paul Désiré Dzeufiet Djomeni ◽

Frida Longo ◽

Pierre Kamtchouing

Keyword(s):

Aqueous Extract ◽

Biochemical Study ◽

Female Rats ◽

Male Rats ◽

Control Group ◽

Control Male ◽

Control Female ◽

Hematological Analysis ◽

Wbc Count ◽

Dose Dependent

Methodology: Wistar rats were randomly assigned into eight groups of five animals each: four male groups and four female groups. Each sex group had a control group receiving distilled water and three test groups receiving 200, 500 and 1000mg/kg respectively. Animal’s body weights were recorded on the first day and once a week for the four experiment weeks. The hematological analysis included total WBC count, total RBC count, Hb, %HCT, MCV, MCH and MCHC. Biochemical/serum profile studies include TG, TC, ALT, AST, urea and TP. Tissue specimens of the liver, kidney and lung were subjected to histological examination using standard hematoxylin-eosin staining.Results: In male rats, aqueous extract showed significant decreases in relative weight of liver with extreme significance P<0.001 at a dose of 200mg/kg (vs. control group), P<0.001 of lung at all the doses, P<0.05 (200 and 500mg/kg) and P<0.01 (1000mg/kg) in heart weight. In relative kidney weight, only the dose of 1000mg/kg showed a significant increase vs. normal control male rats. Unlike male rats, only relative kidney weight in female rats was significantly different from the control group in a dose-dependent manner. The aqueous extract treated male groups showed significant increases P<0.001 (1000mg/kg) of total WBC count and MCHC, significant decreases of %HTC (dose response manner), P<0.05 total RBC count (at doses of 500 and 1000mg/kg) and Hb P<0.01 (500mg/kg) vs. normal male rats. In female rats, the haematological study showed significant increase P<0.01 of total WBC count (at the doses of 500 and 1000mg/kg), significant decreases P<0.05 and P<0.01 of total RBC respectively at the doses of 200 and 1000mg/kg, significant decrease of Hb with extreme significance P<0.001 at the dose 1000mg/kg, %HTC also decrease dose response manner vs. control female rats. Biochemical study showed in male rats significant decreases in level of TG P<0.001 (at the doses of 200 and 500mg/kg) and urea, although it showed any dose-dependent effect vs. control male rats. AST also decreases (P<0.05) in male rats at the dose of 200mg/kg but significantly increase P<0.001 at the dose of 500mg/kg. In the female rats, biochemical study revealed significant increases in level of TG P<0.001 and urea P<0.01 at the dose of 200mg/kg and significant decreases in level of TG P<0.01, AST P<0.05 and urea P<0.05 at the dose of 500mg/kg (vs. control female rats). Microscopically, there were mild hepatic and renal tissue injuries supporting the hematological analysis.Conclusion: The results indicated that aqueous extract of Alstonia boonei De Wild is toxic in high doses.

Download Full-text

Effect of aqueous extract of Date Palm Pollen (DPP) on the sperm characteristic and Serum Testosterone, FSH and LH Values in albino male rats treated with sodium floride

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v38i2.222 ◽

2014 ◽

Vol 38 (2) ◽

pp. 41-47

Author(s):

Salah M. M. AL-Chalabi

Keyword(s):

Aqueous Extract ◽

Date Palm ◽

Sperm Concentration ◽

Serum Testosterone ◽

Male Rats ◽

Maximum Effect ◽

Control Group ◽

Abnormal Sperm ◽

Group 2 ◽

Date Palm Pollen

The present study was undertaken to evaluate the effect of aqueous extract of Date Palm Pollen DPP on the testicular function and serum testosterone, FSH and LH hormones value. Thirty five male rats were divided randomly into five equal groups. Group 1: received 0.5 ml of distilled water (control group), group 2: was treated orally 0.250 p.p.m of sodium florid (NaF) (with volume of 0.5 ml / rat), Group 3: was treated with 0.250 p.p.m of NaF and 50 mg/kg. B.W. of DPP extract (0.5ml D.W \rat), Group 4: was treated with 0.250 p.p.m of NaF and 100 mg/kg. B.W. of DPP extract and Group 5: was treated with 0.250 p.p.m of NaF and 150 mg/kg. B.W. of DPP extract. The results showed significant (P< 0.05) decrease in sperm concentration, motility and significant (P< 0.05) increases in dead and abnormal sperm in the group 2 in comparison to control, while all groups of DPP extract showed significant (P< 0.05) increase in sperm concentration, motility and decrease in dead and abnormal sperm. Maximum effect was observed in animals treated with a dose of 150 mg/kg of DPP extract, also the results revealed significant (P< 0.05) increase in testosterone, FSH and LH hormones in groups treated with DDP in comparison to G1andG2. Male rats received DPP for 50 days showed significant (P< 0.05) increases in body and testes weight as compared to G1andG2. In conclusion the results revealed that the aqueous extract of DPP pollen can be used as a sex enhancer and seems to cure male infertility.

Download Full-text

PROTECTIVE ROLE AND ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF ROSMARINUS OFFICINALIS AGAINST TRICHLOROACETATE-INDUCED TOXICITY IN LIVER OF MALE RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.25353 ◽

2018 ◽

Vol 11 (6) ◽

pp. 420

Author(s):

Medhat Mostafa Abozid ◽

Hoda Ea Farid

Keyword(s):

Aqueous Extract ◽

Liver Damage ◽

Protective Role ◽

Rosmarinus Officinalis ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

Gamma Glutamyl Transferase ◽

Group I ◽

Glutamyl Transferase

Objective: The current study was designed to estimate the potential protective role of the aqueous extract of rosemary (AER) (Rosmarinus officinalis) against trichloroacetic acid (TCA)-created hepatotoxicity in male albino rats.Methods: Forty male albino rats were separated into four groups of ten: Group I served as control; Group II was given AER (200 mg/kg/day) by gavage; Group III received TCA at the dose 50 mg/kg/day, and Group V was treated with AER (200 mg/kg/day) and received TCA (50 mg/kg/day). The experiment was carried out for 2 months.Results: The toxicity of TCA for rats was revealed by an elevation in liver marker enzymes activities (gamma-glutamyl transferase [GGT], alkaline phosphatase [ALP], aspartate transaminase [AST], alanine aminotransferase [ALT]) and conjugated bilirubin (CB) level, and a decrease in albumin and total protein (TP) levels. The TCA administration also caused a significant increase in the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), and also malondialdehyde (MDA) level in liver tissues. These biochemical effects were accompanied by histological indicators of liver damage. Treatment with ARE recovered the liver damage instigated by TCA, as showed by perfection of liver enzyme markers (GGT, ALT, AST, ALP), CB, TP and albumin; as well as antioxidant parameters (CAT, SOD, GPx) and lipid peroxidation (MDA) and amelioration of histopathology changes in the liver tissues.Conclusion: It could be concluded that AER supplementation for 2 months in TCA-induced toxicity in rats benefited hepatic antioxidant status and improved liver injury and damage in male albino rats exposed to TCA.

Download Full-text

Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves

Advances in Pharmacological and Pharmaceutical Sciences ◽

10.1155/2020/3260745 ◽

2020 ◽

Vol 2020 ◽

pp. 1-15

Author(s):

Letiele Bruck de Souza ◽

Amanda Leitão Gindri ◽

Thainara de Andrade Fortes ◽

Thais Felli Kubiça ◽

Jefferson Enderle ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Clinical Isolate ◽

Condensed Tannins ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Phytochemical Study ◽

Carbonyl Protein ◽

Crude Leaf Extract

Context. Chaptalia nutans (L.) Pol. (family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations. Objective. This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C. nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species. Materials and methods. Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P. aeruginosa, B. cereus, E. epidermidis, E. coli, S. aureus, E. faecalis, P. mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C. krusei (clinical isolate), and C. albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature. Toxicity of C. nutans was evaluated using an alternative method, D. melanogaster, as well as a locomotor assay. Results. The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids. A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g). DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C. nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract. Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E. epidermidis, C. tropicalis, C. glabrata, and C. krusei at a concentration of 1000 μg/mL. TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL. Regarding the toxicity of C. nutans extract against D. melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL. Results show that the C. nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity. Conclusion. Our studies demonstrated for the first time that C. nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques. These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D. melanogatser, as well as the ability to neutralize different sources of RONS.

Download Full-text

Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract

Mediterranean Journal of Chemistry ◽

10.13171/mjc02101211565lk ◽

2021 ◽

Vol 11 (1) ◽

pp. 84

Author(s):

Hassan Zrouri ◽

Amine Elbouzidi ◽

Mohamed Bouhrim ◽

Noureddine Bencheikh ◽

Loubna Kharchoufa ◽

...

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Raphanus Sativus ◽

Kidney Diseases ◽

Body Weight Gain ◽

Urinary Calcium ◽

Control Group ◽

Phytochemical Analysis ◽

Distilled Water ◽

Nephroprotective Effect

In Morocco, Raphanus sativus is a widespread traditional medicinal plant used to treat various kidney diseases such as nephropathy.Objective: The present study aims to evaluate the protective effect of the R. sativus aqueous extract against the gentamicin-induced acute nephrotoxicity in rats.Methods: Rats were randomly separated into four groups (n=6; ♂/ ♀=1). The control group was treated only with distilled water (10 mL/kg; p.o). The gentamicin group was treated with distilled water (10 mL/kg; p.o) and injected intraperitoneally by the gentamicin (80 mg/kg; i.p). R. sativus groups were treated with the aqueous extracts of this plant at a dose of (200 or 400 mg/kg; p.o) and injected by the gentamicin (80 mg/kg; i.p). The plasma Creatinine, Urea, Uric Acid, Albumin, Total Protein, Alanine transaminase, Aspartate transaminase, and Calcium levels were measured. The urinary creatinine, urinary Calcium, urinary volume, water intake, creatinine clearance, body weight gain, relative right kidney weight, and kidney malondialdehydes were determined.Results: This study showed that the daily pretreatment with R. sativus aqueous extract at two doses of 200 and 400 mg/kg, p.o prevented the rats from the gentamicin-induced nephrotoxicity. Moreover, the R. sativus aqueous extract showed a high amount of polyphenols and flavonoids and a significant antioxidant activity.Conclusion: R. sativus aqueous extract, as a novel natural product, may have preventive properties against gentamicin-induced nephrotoxicity in rats. The present study describes new areas of investigation to introduce better therapeutic agents for renal disorders and dysfunction.

Download Full-text

Cardioprotective Effect of Ajwa Date Aqueous Extract on Doxorubicin-Induced Toxicity in Rats

Biomedical & Pharmacology Journal ◽

10.13005/bpj/1519 ◽

2018 ◽

Vol 11 (3) ◽

pp. 1521-1536 ◽

Cited By ~ 1

Author(s):

Meaad F. Sabbah ◽

Fawzia Alshubali ◽

Othman A. S. Baothman ◽

Mazin A. Zamzami ◽

Lobna Shash ◽

...

Keyword(s):

Lipid Profile ◽

Aqueous Extract ◽

Chemotherapeutic Agents ◽

Male Rats ◽

Control Group ◽

Dna Integrity ◽

Protective Effects ◽

Cardiac Enzymes ◽

Group Iii ◽

Group I

Doxorubicin (DOX) is one of the most potent and widely used chemotherapeutic agents to treat several malignancies. However, the clinical use of DOX is seriously restricted due to its acute and chronic cardiotoxic side effects This study investigated the protective effect of (Ajwa) date aqueous extract (AJDAE) against doxorubicin-induced cardiotoxicity in rats. Sixty Wister albino male rats (150-200 gms.) were comprised in our study and divided into six equal groups: group I (untreated control), group II, group III, rats were orally received AJDAE (0.75 & 1.5 gm/ kg.bw) respectively, for 4 weeks, rats of groups IV, V and VI were intraperitoneally injected with one dose of doxorubicin (5 mg/kg.bw) at the end of the 4th week of the study to induce cardiotoxicity, rats of groups V & VI were orally received AJDAE (0.75 & 1.5 gm/ kg.bw) respectively. Cardiac enzymes, lipid profile, SOD, GR, GST, GPx, CAT and MDA in rats’ hearts homogenate, urinary 8OHdG as well as DNA integrity and histopathological changes were investigated in all studied rats.Oral administration of AJDAE (0.75 & 1.5 gm/ kg.bw) attenuated the cardiotoxicity of DOX, improved the cardiac enzymes, lipid profile, reduced the urinary 8OHdG and prohibited the depletion of endogenous antioxidants and suppressed lipid peroxidation (MDA). Moreover, AJDAE enhanced DNA integrity. Histological findings showed that AJDAE (0.75 & 1.5 gm/ kg.bw) administration reduced cardiomyocytes alterations, congestion, edema and the intense cellular stress exerted on myocardial fibers as well as restored the cardiomyocytes architecture. Our data showed that AJDAE obviously resulted in protective effects against DOX-induced cardiotoxicity in rat’s heart. It can be concluded that Ajwa date offers a considerable protection against DOX-induced cardiotoxicity.

Download Full-text

NUTRITIONAL AND BIOLOGICAL VALUE OF FRUITS OF CRATAEGUS OXYACANTHA L.

chemistry of plant raw material ◽

10.14258/jcprm.2020015452 ◽

2020 ◽

pp. 265-275

Author(s):

Lyubov' Nikolayevna Skrypnik ◽

Irina Petrovna Melnichuk ◽

Yulia Vladimirovna Koroleva

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Phenolic Compounds ◽

Organic Acids ◽

Food Additives ◽

Raw Material ◽

Transport Infrastructure ◽

Anthropogenic Factors ◽

Total Phenolic ◽

Phytochemical Study

The work involved the phytochemical study of hawthorn fruits (Crataegus oxyacantha L.). Hawthorn fruits were collected in areas with low anthropogenic impact (landscape and recreational areas) and in areas with the transport infrastructure in Kaliningrad city. The content of total phenolic compounds and subgroups: catechins, leucoanthocyanins, flavonoids, tannins); monosaccharides (glucose and fructose), pectins, organic acids, ascorbic acid, macro- and microelements, as well as antioxidant activity in hawthorn fruits were studied. The influence of the anthropogenic factor on the variability of their chemical composition was shown. It has been established that hawthorn fruits from areas with minimal impact of anthropogenic factors can accumulate phenolic compounds (up to 15.9 mg/g), leucoanthocyanins (up to 1.5 mg/g), tannins (up to 6.5 mg/g ), catechins (up to 4.1 mg/g), flavonoids (up to 6.0 mg/g), fructose (up to 14.1%), pectin (up to 11.6%), organic acids (1.45%), ascorbic acid (up to 49.3 mg/100 g), calcium (up to 12.12 mg/g), zinc (up to 39.12 mg/kg) more intensively and demonstrated higher antioxidant activity (up to 9.7 mg/g). The cluster analysis carried out on the content of phytochemicals proved the dependence of the accumulation of nutrients in hawthorn fruits on the growing conditions of plants. The obtained results allow us to consider the fruits of hawthorn C. oxyacantha as a valuable raw material for use as food additives for various purposes.

Download Full-text

Protective Role of Ce Nanoparticles Against the Hepatotoxicity Induced by Exposure to Paraquat

Avicenna Journal of Medical Biochemistry ◽

10.15171/ajmb.2018.09 ◽

2018 ◽

Vol 6 (2) ◽

pp. 37-43

Author(s):

Hamid Heidary Dartoti ◽

Farzin Firozian ◽

Sara Soleimani Asl ◽

Akram Ranjbar

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Protective Role ◽

Male Rats ◽

Hepatic Tissue ◽

Control Group ◽

Serum Samples ◽

Tissue Samples ◽

Total Antioxidant ◽

Group 2

Objectives: The present study aimed to investigate the antioxidant activity of cerium oxide nanoparticles (CeNPs) against paraquat (PQ)-induced liver injury in rats. Methods: Thirty-two male rats were divided into four 8-member groups and treated intraperitoneally with PQ and/or CeNPs for 14 days. Group 1 received PQ (5 mg/kg/d), group 2 received CeNPs (15, 30, and 60 mg/kg/d), group 3 received a combination of PQ (5 mg/kg/d) and CeNPs (15, 30, and 60 mg/kg/d), and group 4 (control group) received saline solution. Serum samples along with liver tissue samples were collected from all the rats. Oxidative stress (OS) biomarkers including total antioxidant capacity, lipid peroxidation, total thiol groups, DNA damage, and nitric oxide levels were determined. Histological samples were also analyzed using hematoxylin and eosin staining slides. Results: Levels of oxidative stress and hepatic tissue damage were significantly higher in the PQ group compared to the control group. CeNPs at a dose of 15 mg/kg showed the antioxidant activity and compromised the PQ-induced damage. Conclusion: In the scenario tested in this study, CeNPs could reduce the levels of OS, as well as hepatic damage induced by PQ.

Download Full-text