scholarly journals Antibacterial Effect and Potency of Jamaican Cherry Leaves (Muntingia calabura L.) as Feed Additive for Antibiotic Growth Promoter Alternative in Animals

2021 ◽  
Vol 31 (3) ◽  
pp. 129
Author(s):  
Anak Agung Gede Fandhiananta Widyanjaya ◽  
Anak Agung Gede Jayawardhita
Keyword(s):  
Antimicrobial Resistance ◽  
Egg Production ◽  
Antibacterial Effect ◽  
Negative Impact ◽  
Bacterial Species ◽  
Feed Additives ◽  
Feed Additive ◽  
Growth Promoter ◽  
Alternative Materials

<p>Antimicrobial resistance is one of the greatest health challenges in the world. The unwise use of Antibiotic Growth Promoter (AGP) in livestock is one of the factors that triggers the increase in the incidence of antimicrobial resistance. In an effort to anticipate the negative impact of using antibiotics, alternative materials are needed to replace AGP. From those various problems, this paper aims to provide information on the use of phytogenic feed additives as natural antibacterials that have the potency as an alternative AGP. Based on the results of various studies, Jamaican cherry leaf extract has the ability to inhibit the growth of various bacterial species <em>in vitro</em>. Various phytochemical test results showed that Jamaican cherry leaves contain several types of phenolics, flavonoids, tannins, and saponins that have antibacterial effect.  The addition of Jamaican cherry leaves to feed was able to increase the value of digestibility, carcass weight, egg production, and protein content of meat. Therefore, Jamaican cherry leaf (<em>Muntingia carabula</em> L.) has the potency to be used as feed additive to replace AGP.</p>

Cell-free supernatants produced by lactic acid bacteria reduce Salmonella population in vitro

Microbiology ◽  
2021 ◽  
Vol 167 (11) ◽  
Author(s):  
Alberto Gonçalves Evangelista ◽  
Jessica Audrey Feijó Corrêa ◽  
João Vitor Garcia dos Santos ◽  
Eduardo Henrique Custódio Matté ◽  
Mônica Moura Milek ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Antimicrobial Resistance ◽  
Lactic Acid Bacteria ◽  
Type Species ◽  
Feed Additives ◽  
Poultry Feed ◽  
Content Type ◽  
Link Type

The genus Salmonella is closely associated with foodborne outbreaks and animal diseases, and reports of antimicrobial resistance in Salmonella species are frequent. Several alternatives have been developed to control this pathogen, such as cell-free supernatants (CFS). Our objective here was to evaluate the use of lactic acid bacteria (LAB) CFS against Salmonella in vitro. Seventeen strains of LAB were used to produce CFS, and their antimicrobial activity was screened towards six strains of Salmonella . In addition, CFS were also pH-neutralized and/or boiled. Those with the best results were lyophilized. MICs of lyophilized CFS were 11.25–22.5 g l–1. Freeze-dried CFS were also used to supplement swine and poultry feed (11.25 g kg–1) and in vitro simulated digestion of both species was performed, with Salmonella contamination of 5×106 and 2×105 c.f.u. g−1 of swine and poultry feed, respectively. In the antimicrobial screening, all acidic CFS were able to inhibit the growth of Salmonella . After pH neutralization, Lactobacillus acidophilus Llorente, Limosilactobacillus fermentum CCT 1629, Lactiplantibacillus plantarum PUCPR44, Limosilactobacillus reuteri BioGaia, Lacticaseibacillus rhamnosus ATCC 7469 and Pediococcus pentosaceus UM116 CFS were the only strains that partially maintained their antimicrobial activity and, therefore, were chosen for lyophilization. In the simulated swine digestion, Salmonella counts were reduced ≥1.78 log c.f.u. g–1 in the digesta containing either of the CFS. In the chicken simulation, a significant reduction was obtained with all CFS used (average reduction of 0.59±0.01 log c.f.u. ml–1). In general, the lyophilized CFS of L. fermentum CCT 1629, L. rhamnosus ATCC 7469 and L. acidophilus Llorente presented better antimicrobial activity. In conclusion, CFS show potential as feed additives to control Salmonella in animal production and may be an alternative to the use of antibiotics, minimizing problems related to antimicrobial resistance.

scholarly journals Review: Peluang Pengembangan Imbuhan Pakan Fitogenik Sebagai Pengganti Antibiotika dalam Ransum Ayam Pedaging di Indonesia

2019 ◽  
Vol 6 (2) ◽  
pp. 188
Author(s):  
Cecep Hidayat ◽  
Rahman Rahman
Keyword(s):  
Broiler Chicken ◽  
Scientific Literature ◽  
Feed Additives ◽  
Feed Additive ◽  
Growth Promoter ◽  
Literature Study ◽  
The Third ◽  
Feed Industry ◽  
Broiler Diet ◽  
Main Support

ABSTRAK                                                            Seiring dengan telah dilarangnya penggunaan antibiotic growth promotor (AGP) dalam pakan oleh pemerintah Indonesia, maka pencarian pengganti AGP mejadi penting saat ini. Imbuhan pakan fitogenik menjadi salah satu pengganti AGP potensial. Makalah ini ditulis untuk mengungkap peluang pengembangan imbuhan pakan fitogenik sebagai pengganti AGP dalam ransum ayam pedaging di Indonesia. Metode penulisan makalah dilakukan dengan beberapa tahapan. Tahapan pertama, tahap persiapan, dilakukan dengan mengumpulkan dan membaca jurnal, makalah prosiding, buku yang diperoleh dari hasil dari pencarian  pada internet. Dilanjutkan dengan memilih dan menentukan jurnal hasil penelitian, makalah ilmiah pada prosiding, buku terkait dengan topik yang sudah ditentukan. Tahapan ke dua, penyusunan outline makalah yang akan ditulis, dimana pada tahapan ini untuk menentukan skema berfikir penulis terkait dengan topik yang akan di bahas. Tahapan ke tiga, mengembangkan kerangka makalah dengan menggunakan literatur ilmiah, teori, hasil penelitian yang sudah diperoleh. Tahapan ke empat, pemeriksaan terhadap isi makalah yang sudah ditulis, baik secara substansi maupun redaksional. Berdasarkan studi pustaka yang dilakukan disimpulkan bahwa imbuhan pakan fitogenik dari berbagai jenis tanaman potensial digunakan sebagai pengganti AGP dalam pakan ayam pedaging. Kebutuhan akan daging ayam nasional yang semakin tinggi setiap tahunnya, menjadi peluang betapa besarnya pasar akan kebutuhan pengganti AGP di Indonesia. Sinergisme antara lembaga penelitian-perguruan tinggi dengan pelaku industri pakan dan imbuhan pakan menjadi penopang utama dalam pengembangan imbuhan pakan fitogenik di Indonesia.Kata Kunci: imbuhan pakan fitogenik, ayam pedagingABSTRACTKeywords: pytogenic feed additive, broiler chicken The use of antibiotic growth promoter (AGP) in feed has been banned by the Indonesian government. Therefore, the search for alternative AGP continues to be carried out. The addition of phytogenic is one of the potential alternatives because it has the same ability as AGP. This paper was written to reveal the opportunities for developing phytogenic feed additives as a substitute for antibiotics in broiler diet in Indonesia. The method of writing this paper was done in several stages. The first stage, the preparation, is done by searching and reading journals, proceedings, books obtained from the internet. The second method is the preparation of the outline of the paper to be written, to determine the author's thinking scheme related to the topic to be discussed. The third method is developing a framework of thought by using scientific literature obtained and read before. Fourth method is examination of the contents of papers that have been written, both substantially and editorially. Based on the literature study, it was concluded that the phytogenic feed additives from various types of plants should be considered as a substitute for AGP in broiler diet.  The need for national chicken meat which is getting higher every year becomes an opportunity for how big the market will be for AGP's replacement in Indonesia. Synergism between university-research institutions and feed industry is the main support in developing phytogenic feed additives in Indonesia.Keywords: pytogenic feed additive, broiler chicken

scholarly journals Antimicrobial Susceptibility Testing and Tentative Epidemiological Cutoff Values for FiveBacillusSpecies Relevant for Use as Animal Feed Additives or for Plant Protection

2018 ◽  
Vol 84 (19) ◽  
Author(s):  
Yvonne Agersø ◽  
Birgitte Stuer-Lauridsen ◽  
Karin Bjerre ◽  
Michelle Geervliet Jensen ◽  
Eric Johansen ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Animal Feed ◽  
Bacterial Species ◽  
Feed Additives ◽  
Content Type ◽  
Cutoff Values ◽  
Antimicrobial Resistance Genes ◽  
Species Specific

ABSTRACTBacillus megaterium(n= 29),Bacillus velezensis(n= 26),Bacillus amyloliquefaciens(n= 6),Bacillus paralicheniformis(n= 28), andBacillus licheniformis(n= 35) strains from different sources, origins, and time periods were tested for the MICs for nine antimicrobial agents by the CLSI-recommended method (Mueller-Hinton broth, 35°C, for 18 to 20 h), as well as with a modified CLSI method (Iso-Sensitest [IST] broth, 37°C [35°C forB. megaterium], 24 h). This allows a proposal of species-specific epidemiological cutoff values (ECOFFs) for the interpretation of antimicrobial resistance in these species. MICs determined by the modified CLSI method were 2- to 16-fold higher than with the CLSI-recommended method for several antimicrobials. The MIC distributions differed between species for five of the nine antimicrobials. Consequently, use of the modified CLSI method and interpretation of resistance by use of species-specific ECOFFs is recommended. The genome sequences of all strains were determined and used for screening for resistance genes against the ResFinder database and for multilocus sequence typing. A putative chloramphenicol acetyltransferase (cat) gene was found in oneB. megateriumstrain with an elevated chloramphenicol MIC compared to the otherB. megateriumstrains. InB. velezensisandB. amyloliquefaciens, a putative tetracycline efflux gene,tet(L), was found in all strains (n= 27) with reduced tetracycline susceptibility but was absent in susceptible strains. AllB. paralicheniformisand 23% ofB. licheniformisstrains had elevated MICs for erythromycin and harboredermD. The presence of these resistance genes follows taxonomy suggesting they may be intrinsic rather than horizontally acquired. Reduced susceptibility to chloramphenicol, streptomycin, and clindamycin could not be explained in all species.IMPORTANCEWhen commercializing bacterial strains, likeBacillusspp., for feed applications or plant bioprotection, it is required that the strains are free of acquired antimicrobial resistance genes that could potentially spread to pathogenic bacteria, thereby adding to the pool of resistance genes that may cause treatment failures in humans or animals. Conversely, if antimicrobial resistance is intrinsic to a bacterial species, the risk of spreading horizontally to other bacteria is considered very low. Reliable susceptibility test methods and interpretation criteria at the species level are needed to accurately assess antimicrobial resistance levels. In the present study, tentative ECOFFs for fiveBacillusspecies were determined, and the results showed that the variation in MICs followed the respective species. Moreover, putative resistance genes, which were detected by whole-genome sequencing and suggested to be intrinsic rather that acquired, could explain the resistance phenotypes in most cases.

Combined Inhibitory Effect of Curcuma Xanthorrhiza Extract and Xylitol on Streptococcus Mutans and Actinomyces Viscosus

2007 ◽  
Vol 342-343 ◽  
pp. 861-864
Author(s):  
Hae Sun Kim ◽  
Choong Ho Choi ◽  
H.K. Kwon ◽  
B.I. Kim
Keyword(s):  
Antibacterial Effect ◽  
Bacterial Species ◽  
Inhibitory Effect ◽  
Distilled Water ◽  
Phenol Red ◽  
Ph Indicator ◽  
Bacterial Fermentation ◽  
Curcuma Xanthorrhiza ◽  
Growth Of Bacteria

This study evaluated the combined inhibitory effects of a Curcuma xanthorrhiza extract (CXE) and Xylitol on S. mutans and A. viscosus in vitro. Three series of experiments on S. mutans and A. viscosus were carried out. In the first series, the Minimum inhibitory concentrations (MICs) of CXE, Xylitol, and CXE mixed with Xylitol (CXE+Xylitol) against S. mutans and A. viscosus were determined. Second, the antibacterial effect and the rapid effectiveness of CXE, Xylitol, and CXE+Xylitol against those bacteria was evaluated as contacting for 1, 2, 5, and 10 minutes. Finally, The saccharolytic capability of S. mutans was examined using bovine teeth that had been pretreated with CXE (1%), Xylitol (1%), CXE+Xylitol (1%), chlorhexidine (1%) and distilled water, and rinsed with distilled water. The pretreated bovine teeth were layered with soft agar containing sucrose (5%), S. mutans and phenol red, as a pH indicator, and incubated. The MICs of CXE were 5 ppm on both bacterial species. Xylitol did not inhibit either species. The MICs of CXE+Xylitol were 10 and 5 ppm against S. mutans, A. viscosus, respectively. According to the rapid effectiveness, CXE completely inhibited the growth of bacteria but Xylitol did not. CXE+Xylitol could completely inhibit the growth of bacteria. An evaluation of the saccharolytic capability of S. mutans on bovine teeth revealed that distilled water and Xylitol could not inhibit bacterial fermentation. However, the bovine teeth containing CXE, CXE+Xylitol and chlorhexidine inhibited the fermentation of bacteria. These results show that CXE and CXE+Xylitol have a strong antibacterial effect on S. mutans and A. viscosus in vitro.

scholarly journals Microwave Mediated Synthesis of Novel 1,3,5-Triazine Derivative and their Biological Evaluation

2021 ◽  
Vol 3 (12) ◽  
pp. 181-185
Author(s):  
Krishn Kumar Barmase ◽  
Deepak Basedia ◽  
Balkrishna Dubey
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Bacterial Species ◽  
Biological Evaluation ◽  
Chloride Ions ◽  
Gram Negative ◽  
Triazine Derivative ◽  
Triazine Derivatives ◽  
In Vitro Antibacterial Activity

1,3,5-Triazine derivative are synthesized by replacement of chloride ions of Cyanuric chloride and 1,3,5-Triazine derivative are showing promising biologically activity such as Antibacterial, Antifungal, Antimalarial, Antivirus, Anticancer that’s why interest of researches on synthesis of 1,3,5-Triazine derivatives always in focused. The present study reported the synthesis of 2,4,6-Trisubstituted 1,3,5-Triazine derivatives by Microwave mediated Method which gave the desired result in less time with higher yield. The structure of 1,3,5-Triazine derivatives have been elucidated by Spectral of IR, NMR and MASS. The derivative of 1,3,5-Triazine are evaluated for their In vitro Antibacterial activity against Gram Positive and Gram Negative Bacterial species and shown good Antibacterial effect.

scholarly journals PENGARUH PENAMBAHAN TEPUNG KUNYIT DAN BETAIN PADA RANSUM TERHADAP BERAT KARKAS, TEBAL LEMAK PUNGGUNG DAN LOIN EYE AREA BABI FINISHER (Effect of Dietary Turmeric Flour and Betain Addition on Carcass Weight, Backfat Thickness and Loin Eye Area of Finisher Pig)

2020 ◽  
Vol 8 (1) ◽  
pp. 45-50
Author(s):  
Jimmi RH Sinaga ◽  
Sauland Sinaga ◽  
Denny Rusmana
Keyword(s):  
Feed Additives ◽  
Carcass Weight ◽  
Backfat Thickness ◽  
Feed Additive ◽  
Growth Promoter ◽  
Randomized Design ◽  
Body Weights ◽  
Completely Randomized Design ◽  
Pig Carcasses ◽  
Different Doses

Antibiotic Growth Promoter (AGP) synthetic as a feed additive can stimulate growth, prevent some health problems, and improve feed efficiency. However, its journey has raised concerns due to adverse effects such as residues and microbial resistance. The use of turmeric flour and betaine as feed additives is expected to substitute the use of AGP Synthetic in improving the performance of pig carcasses and the safety of consumption. This study was aimed to examine the effect of the addition of turmeric flour and different doses of betaine in the ration on the performance of finisher period pigs. The study was conducted experimentally using a completely randomized design with four treatments and five replications. The objects of the study were 20 male and female finisher Landrace pigs with a finisher period with initial body weights of 55-66 kg/head. The study rations used were R0: basal ration, R1: R0 + 0.4% turmeric flour + 0.1% betaine, R2: R0 + 0.4% turmeric flour + 0.15% betaine and R3: R0 + 0.4% turmeric flour + 0.2% betaine. The parameters measured were carcass weight, backfat thickness, and loin eye area. The addition of turmeric flour treatment did not have any significant effects on carcass weight and thickness of the back fat of the pig. The addition of 0.4% turmeric flour and 0.15% betaine in the ration was the best treatment and resulted in a different effect on the loin eye area compared to that of R0, while treatment R1 and R3 had a similar effect on it. The result of the addition of 0.4% turmeric flour and 0.15% betaine resulted in the loin eye area of the finisher pig of 53.4 cm2

scholarly journals Kinetics of In Vitro Gas Production and Fitting Mathematical Models of Corn Silage

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 298
Author(s):  
Camila da Silva Zornitta ◽  
Luis Carlos Vinhas Ítavo ◽  
Camila Celeste Brandão Ferreira Ítavo ◽  
Geraldo Tadeu dos Santos ◽  
Alexandre Menezes Dias ◽  
...  
Keyword(s):  
Mathematical Models ◽  
Gas Production ◽  
Feed Additives ◽  
Corn Silage ◽  
Feed Additive ◽  
Coefficient Of Determination ◽  
Production Data ◽  
In Vitro Gas Production ◽  
Kinetics Of

This study aimed at examining the effects of rumen inoculum of steers receiving different combinations of ionophore and probiotics in their diets on in vitro gas production of corn silage. The fitting of gas production was performed with five mathematical models and its kinetics was evaluated. Four crossbred steers (403.0 ± 75.5 kg body weight) with ruminal cannula were assigned to a 4 × 4 Latin square design. The additives used were Monensin sodium (Rumensin® 100, 3 g/day), Bacillus toyonensis (Micro-Cell Platinum® 109, 1 g/day) and Saccharomyces cerevisiae boulardii (ProTernative®20, 0.5 g/day). Additives were arranged into the following treatments, supplied daily into total mixed diet: (1) Monensin; (2) Monensin + B. toyonensis; (3) Monensin + S. boulardii; and (4) B. toyonensis + S. boulardii. The gas production data were fitted into the models of Gompertz, Groot, Ørskov, Brody, Richards, and Dual-pool Logistic. A perfect agreement between observed and predicted values in curves of accumulated in vitro gas production was observed in the Groot and Richards models, with higher coefficient of determination (R2 = 0.770 and 0.771, respectively), concordance correlation coefficient (CCC = 0.871 and 0.870, respectively), and root mean square error of prediction (RMSEP = 1.14 and 1.15, respectively). Evaluating the feed additives throughout the Groot model, the B. toyonensis + S. boulardii treatment presented higher VF (12.08 mL/100 mg of DM; p = 0.0022) than Monensin and Monensin + S. boulardii (9.16 and 9.22 mL/100 mg of DM, respectively). In addition, the fractional rate of gas production (k) was higher (p = 0.0193) in B. toyonensis + S. boulardii than in Monensin, not presenting a statistical difference (p > 0.05) from the other two treatments. Additionally, with the time of beginning to gas production, the lag time (λ), was greater (p < 0.001) with Monensin and Monensin + B. toyonensis than with Monensin + S. boulardii and B. toyonensis + S. boulardii. The combination of Monensin and probiotics (B. toyonensis + S. boulardii) resulted in better kinetics of degradation of corn silage, being that the Groot and Richards models had the best fit for estimates of the in vitro gas production data of corn silage tested with different feed additive combinations.

scholarly journals Antibacterial effects of nanoimprinted moth-eye film in practical settings

2018 ◽  
Author(s):  
Miho Yamada ◽  
Kiyoshi Minoura ◽  
Takashi Mizoguchi ◽  
Kenichiro Nakamatsu ◽  
Tokio Taguchi ◽  
...  
Keyword(s):  
Antibacterial Effect ◽  
Culture Media ◽  
Bacterial Species ◽  
General Purpose ◽  
Antibacterial Effects ◽  
Hand Wash ◽  
Flat Film ◽  
Pet Film ◽  
Nanofabrication Technique

AbstractRecent studies report that surfaces displaying micrometer-or nanometer-sized undulating structures exhibit antibacterial effects. In previous work, we described the use of an advanced nanofabrication technique to generate an artificial biomimetic moth-eye film by coating a polyethylene terephthalate (PET) film with nanoscale moth-eye protrusions made from a hydrophilic resin. This moth-eye film exhibited enhanced antibacterial effects in in vitro experiments. The aim of the present study was to verify the antibacterial efficacy of the Moth-eye film in practical environments. Three types of films (Moth-eye film, Flat film, and PET film) were used to compare antibacterial effects. Sample films were pasted onto hand-wash sinks at the testing locations. After several hours of elapsed time, bacteria from the surface of sample films were collected using one of three kinds of culture media stampers (to permit identification of bacterial species). The stampers were incubated for 48 hours at 35 °C, and the numbers of colonies were counted.The number of common bacteria including E. coli and S. aureus from the Moth-eye film was significantly lower than that from the PET film (p<0.05) and that from the Flat film at 1 hour (p<0.05). This study found that the Moth-eye film had durability of antibacterial effect and the Moth-eye structure (PET coated with nanoscale cone-shaped pillars) had a physical antibacterial effect from the earlier time points. Therefore, the Moth-eye film might be useful for general-purpose applications in practical environments.

scholarly journals In vitro immunomodulatory effects of thymol and cinnamaldehyde in a pig intestinal epithelial cell line (IPEC-J2)

2020 ◽  
Vol 8 (3) ◽  
pp. 127-134
Author(s):  
C. Shen ◽  
L.G. Christensen ◽  
P.B. Rasmussen ◽  
K.M. Kragh
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Tight Junctions ◽  
Feed Additives ◽  
Cellular Damage ◽  
Feed Additive ◽  
Epithelial Cell Line ◽  
Rt Pcr ◽  
Wound Recovery

Thymol and cinnamaldehyde are phytogenic feed additives developed to improve gut health and growth performance in poultry and swine. This study evaluated the in vitro immune modulating effects of thymol and cinnamaldehyde blend (TCB) in a porcine gut epithelial cell line (IPEC-J2), with or without cellular damage caused by challenge with lipopolysaccharides. Cytotoxicity, permeability, wound-healing and bacteria adhesion assays were recorded. The expression of cytokines, tight junctions and polymeric immunoglobulin receptor (pIgR) were measured by RT-PCR. The IPEC-J2 cells were cultured in the presence of TCB at concentrations ranging from 1 ng/ml to 1 μg/ml and displayed high viability (>90%). TCB increased barrier integrity (13.8% less in lipopolysaccharide challenge which induced gut epithelial leakage, P<0.05) and accelerated the initial speed of wound recovery (day 1, 26% wound recovery in TCB treated vs 7% in control, P<0.05; day 2, 54 vs 39%, P<0.001). The RT-PCR analysis of cell culture showed that TCB upregulated anti-inflammatory cytokine interleukin (IL)-10 (73.3%, P<0.05) in non-stimulated IPEC-J2 cells, while, when stimulated, pIgR (9.7%, P<0.05) and tight junctions claudin-4 (9.4%, P<0.05) were upregulated by TCB. Furthermore, TCB significantly increased Lactobacillus acidophilus adherence to gut epithelial cells (285.0%, P<0.05). Overall, the current in vitro study showed that TCB can induce various immune responses, which may explain its in vivo benefits as feed additive.

Influence of enzyme feed additive on the quality of eggs on the example of PJSC “Poultry plant Chelyabinskaya”

2020 ◽  
pp. 52-59
Author(s):  
S. Ermolov ◽  
V. Kosilov ◽  
Yu. Barabanova
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Labour Productivity ◽  
Feed Additives ◽  
Poultry Meat ◽  
Feed Additive ◽  
Simultaneous Increase ◽  
Control Group ◽  
Experimental Group

Poultry farming is one of the major branches of animal husbandry, which develops through concentration, specialization and intensification on the basis of poultry plants and poultry farms. The increase in production of eggs and poultry meat is based on a significant increase in the productivity of poultry with a simultaneous increase in its population with a high payment of feed by production and the increase in labour productivity. The purpose of the work was to study the influence of enzyme feed additives in the diets of laying hens on the quality of eggs. The researches have been carried out in PJSC “Poultry plant Chelyabinskaya”, which produces high-quality chicken eggs obtained from the poultry cross “Loman LSL-classic”. The object of study was enzyme drugs produced by Sunzyme – a multi-enzyme drug that includes xylanase, cellulase and mannase activities. The dosage of enzyme drugs in the feed mixture of laying hens was: Sunfyze 5000 – 80 g/t, Sunzyme – 100 g/t. The results of researches show that in the groups that received enzyme drugs in their diet, egg production was higher than in the control group. So, for the entire period of researches the hens of the experimental groups were significantly superior to the control group in terms of egg production per average laying hen. The largest difference was in hens of the 1st experimental group (enzyme drug Sunfyze) – 10,8 eggs; the smallest in hens of the 3rd group (enzyme drugs Sunfyze and Sunzyme) – 5,7 eggs. In hens of the 2nd experimental group compared with hens of the control group egg production was higher by 7,9 eggs. The best indicators of production efficiency have been obtained in the 1st experimental group of laying hens, which were fed with the enzyme feed additive Sunfyze to reduce the nutritional value of the diet according to the matrix.

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