Collagen as a Biomaterial: An Application in Knee Meniscal Fibrocartilage Regeneration

1993 ◽  
Vol 331 ◽  
Author(s):  
Shu-Tung Li ◽  
Debbie Yuen ◽  
Peggy C. Li ◽  
William G. Rodkey ◽  
Kevin R. Stone
Keyword(s):  
Knee Joint ◽  
Tissue Regeneration ◽  
Tissue Repair ◽  
Biomedical Applications ◽  
Biological Properties ◽  
Design And Development ◽  
Safety And Efficacy ◽  
Meniscal Tissue ◽  
Efficacy Studies

AbstractFibrous type I collagen possesses unique physicochemical, mechanical and biological properties which are useful for biomedical applications. Our research in applying collagen materials for various tissue repair has led to the development of a resorbable collagen based implant for meniscal tissue regeneration applications. We discuss here the design and development of this resorbable collagen based implant. The results of safety and efficacy studies indicate that the collagen based implant, if designed properly, can be a safe and effective template to support meniscal tissue regeneration in the knee joint.

Sulfated Polysaccharides from Macroalgae for Bone Tissue Regeneration

2019 ◽  
Vol 25 (11) ◽  
pp. 1200-1209 ◽  
Author(s):  
Jayachandran Venkatesan ◽  
Sukumaran Anil ◽  
Sneha Rao ◽  
Ira Bhatnagar ◽  
Se-Kwon Kim
Keyword(s):  
Bone Tissue ◽  
Bioactive Compounds ◽  
Tissue Regeneration ◽  
Tissue Repair ◽  
Biomedical Applications ◽  
Stem Cell Differentiation ◽  
Bone Tissue Regeneration ◽  
Sulfated Polysaccharides ◽  
Tissue Repair And Regeneration ◽  
Pharmaceutical Industries

Background: Utilization of macroalgae has gained much attention in the field of pharmaceuticals, nutraceuticals, food and bioenergy. Macroalgae has been widely consumed in Asian countries as food from ancient days and proved that it has potential bioactive compounds which are responsible for its nutritional properties. Macroalgae consists of a diverse range of bioactive compounds including proteins, lipids, pigments, polysaccharides, etc. Polysaccharides from macroalgae have been utilized in food industries as gelling agents and drug excipients in the pharmaceutical industries owing to their biocompatibility and gel forming properties. Exploration of macroalgae derived sulfated polysaccharides in biomedical applications is increasing recently. Method: In the current review, we have provided information of three different sulfated polysaccharides such as carrageenan, fucoidan and ulvan and their isolation procedure (enzymatic precipitation, microwave assisted method, and enzymatic hydrolysis method), structural details, and their biomedical applications exclusively for bone tissue repair and regeneration. Results: From the scientific results on sulfated polysaccharides from macroalgae, we conclude that sulfated polysaccharides have exceptional properties in terms of hydrogel-forming ability, scaffold formation, and mimicking the extracellular matrix, increasing alkaline phosphatase activity, enhancement of biomineralization ability and stem cell differentiation for bone tissue regeneration. Conclusion: Overall, sulfated polysaccharides from macroalgae may be promising biomaterials in bone tissue repair and regeneration.

scholarly journals Adipose-Derived Mesenchymal Cells for Bone Regereneration: State of the Art

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Marta Barba ◽  
Claudia Cicione ◽  
Camilla Bernardini ◽  
Fabrizio Michetti ◽  
Wanda Lattanzi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Biomedical Applications ◽  
State Of The Art ◽  
Biological Properties ◽  
Clinical Applications ◽  
Structure And Function ◽  
Tissue Reconstruction ◽  
And Function

Adipose tissue represents a hot topic in regenerative medicine because of the tissue source abundance, the relatively easy retrieval, and the inherent biological properties of mesenchymal stem cells residing in its stroma. Adipose-derived mesenchymal stem cells (ASCs) are indeed multipotent somatic stem cells exhibiting growth kinetics and plasticity, proved to induce efficient tissue regeneration in several biomedical applications. A defined consensus for their isolation, classification, and characterization has been very recently achieved. In particular, bone tissue reconstruction and regeneration based on ASCs has emerged as a promising approach to restore structure and function of bone compromised by injury or disease. ASCs have been used in combination with osteoinductive biomaterial and/or osteogenic molecules, in either static or dynamic culture systems, to improve bone regeneration in several animal models. To date, few clinical trials on ASC-based bone reconstruction have been concluded and proved effective. The aim of this review is to dissect the state of the art on ASC use in bone regenerative applications in the attempt to provide a comprehensive coverage of the topics, from the basic laboratory to recent clinical applications.

scholarly journals Sericin based nanoformulations: a comprehensive review on molecular mechanisms of interaction with organisms to biological applications

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Gitishree Das ◽  
Han-Seung Shin ◽  
Estefânia V. Ramos Campos ◽  
Leonardo Fernandes Fraceto ◽  
Maria del Pilar Rodriguez-Torres ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Biomedical Applications ◽  
Molecular Mechanisms ◽  
Low Cost ◽  
Pharmaceutical Formulations ◽  
Biological Properties ◽  
Regulation Of Transcription ◽  
Main Body ◽  
Silkworm Cocoon ◽  
Stimulation Of

Abstract Background The advances in products based on nanotechnology have directed extensive research on low-cost, biologically compatible, and easily degradable materials. Main body Sericin (SER) is a protein mainly composed of glycine, serine, aspartic acid, and threonine amino acids removed from the silkworm cocoon (particularly Bombyx mori and other species). SER is a biocompatible material with economic viability, which can be easily functionalized due to its potential crosslink reactions. Also, SER has inherent biological properties, which makes possible its use as a component of pharmaceutical formulations with several biomedical applications, such as anti-tumor, antimicrobials, antioxidants and as scaffolds for tissue repair as well as participating in molecular mechanisms attributed to the regulation of transcription factors, reduction of inflammatory signaling molecules, stimulation of apoptosis, migration, and proliferation of mesenchymal cells. Conclusion In this review, the recent innovations on SER-based nano-medicines (nanoparticles, micelles, films, hydrogels, and their hybrid systems) and their contributions for non-conventional therapies are discussed considering different molecular mechanisms for promoting their therapeutic applications.

The Potential of Fucose-Containing Sulfated Polysaccharides As Scaffolds for Biomedical Applications

2019 ◽  
Vol 26 (35) ◽  
pp. 6399-6411 ◽  
Author(s):  
Cláudia Nunes ◽  
Manuel A. Coimbra
Keyword(s):  
Tissue Regeneration ◽  
Cell Walls ◽  
Biomedical Applications ◽  
Structural Features ◽  
Therapeutic Agents ◽  
Sulfated Polysaccharides ◽  
Clinical Use ◽  
Health Related ◽  
Pathogen Inhibition ◽  
Health Applications

Marine environments have a high quantity and diversity of sulfated polysaccharides. In coastal regions brown algae are the most abundant biomass producers and their cell walls have fucosecontaining sulfated polysaccharides (FCSP), known as fucans and/or fucoidans. These sulfated compounds have been widely researched for their biomedical properties, namely the immunomodulatory, haemostasis, pathogen inhibition, anti-inflammatory capacity, and antitumoral. These activities are probably due to their ability to mimic the carbohydrate moieties of mammalian glycosaminoglycans. Therefore, the FCSP are interesting compounds for application in health-related subjects, mainly for developing scaffolds for delivery systems or tissue regeneration. FCSP showed potential for these applications also due to their ability to form stable 3D structures with other polymers able to entrap therapeutic agents or cell and growth factors, besides their biocompatibility and biodegradability. However, for the clinical use of these biopolymers well-defined reproducible molecules are required in order to accurately establish relationships between structural features and human health applications.

scholarly journals Polysaccharide-Based Aerogel Production for Biomedical Applications: A Comparative Review

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1631
Author(s):  
Mariangela Guastaferro ◽  
Ernesto Reverchon ◽  
Lucia Baldino
Keyword(s):  
Tissue Regeneration ◽  
Supercritical Co2 ◽  
Biomedical Applications ◽  
Freeze Drying ◽  
Time Interval ◽  
Comparative Review ◽  
Biomedical Field ◽  
Low Cytotoxicity ◽  
Gel Preparation ◽  
Cell Adhesion And Proliferation

A comparative analysis concerning bio-based gels production, to be used for tissue regeneration, has been performed in this review. These gels are generally applied as scaffolds in the biomedical field, thanks to their morphology, low cytotoxicity, and high biocompatibility. Focusing on the time interval 2015–2020, the production of 3D scaffolds of alginate, chitosan and agarose, for skin and bone regeneration, has mainly been investigated. Traditional techniques are critically reviewed to understand their limitations and how supercritical CO2-assisted processes could overcome these drawbacks. In particular, even if freeze-drying represents the most widespread drying technique used to produce polysaccharide-based cryogels, supercritical CO2-assisted drying effectively allows preservation of the nanoporous aerogel structure and removes the organic solvent used for gel preparation. These characteristics are essential for cell adhesion and proliferation.

scholarly journals Bacterial Nanocellulose in Dentistry: Perspectives and Challenges

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 49
Author(s):  
Hélida Gomes de Oliveira Barud ◽  
Robson Rosa da Silva ◽  
Marco Antonio Costa Borges ◽  
Guillermo Raul Castro ◽  
Sidney José Lima Ribeiro ◽  
...  
Keyword(s):  
Tissue Repair ◽  
Biomedical Applications ◽  
Low Cost ◽  
Pulp Tissue ◽  
Bacterial Nanocellulose ◽  
Artificial Blood ◽  
Current Trends ◽  
Artificial Blood Vessels ◽  
Near Future ◽  
Dressing Materials

Bacterial cellulose (BC) is a natural polymer that has fascinating attributes, such as biocompatibility, low cost, and ease of processing, being considered a very interesting biomaterial due to its options for moldability and combination. Thus, BC-based compounds (for example, BC/collagen, BC/gelatin, BC/fibroin, BC/chitosan, etc.) have improved properties and/or functionality, allowing for various biomedical applications, such as artificial blood vessels and microvessels, artificial skin, and wounds dressing among others. Despite the wide applicability in biomedicine and tissue engineering, there is a lack of updated scientific reports on applications related to dentistry, since BC has great potential for this. It has been used mainly in the regeneration of periodontal tissue, surgical dressings, intraoral wounds, and also in the regeneration of pulp tissue. This review describes the properties and advantages of some BC studies focused on dental and oral applications, including the design of implants, scaffolds, and wound-dressing materials, as well as carriers for drug delivery in dentistry. Aligned to the current trends and biotechnology evolutions, BC-based nanocomposites offer a great field to be explored and other novel features can be expected in relation to oral and bone tissue repair in the near future.

MPA-capped CdTe quantum dots exposure causes neurotoxic effects in nematode Caenorhabditis elegans by affecting the transporters and receptors of glutamate, serotonin and dopamine at the genetic level, or by increasing ROS, or both

Nanoscale ◽  
2015 ◽  
Vol 7 (48) ◽  
pp. 20460-20473 ◽  
Author(s):  
Tianshu Wu ◽  
Keyu He ◽  
Qinglin Zhan ◽  
Shengjun Ang ◽  
Jiali Ying ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Caenorhabditis Elegans ◽  
Biomedical Applications ◽  
Biological Properties ◽  
Cdte Quantum Dots ◽  
Nematode Caenorhabditis Elegans ◽  
Neurotoxic Effects ◽  
Genetic Level

As quantum dots (QDs) are widely used in biomedical applications, the number of studies focusing on their biological properties is increasing.

Viscoelastic Properties of Genetically Engineered Silk-Elastin-Like Protein Polymers

2008 ◽  
Author(s):  
Weibing Teng ◽  
Joseph Cappello ◽  
Xiaoyi Wu
Keyword(s):  
Drug Delivery ◽  
Viscoelastic Properties ◽  
Biomedical Applications ◽  
Biological Properties ◽  
Genetically Engineered ◽  
Structural Proteins ◽  
Design And Synthesis ◽  
Cross Links ◽  
New Protein ◽  
Polypeptide Sequences

Genetic engineering of protein-based materials provides material scientists with high levels of control in material microstructures, properties, and functions [1]. For example, multi-block protein copolymers in which individual block may possess distinct mechanical or biological properties have been biosynthesized [2, 3]. Polypeptide sequences derived from well-studied structural proteins (e.g., collagen, silk, elastin) are often used as motifs in the design and synthesis of new protein-based material, in which new functional groups may be incorporated. In this fashion, we have produced a series of silk-elastin-like proteins (SELPs) consisting of polypeptide sequences derived from silk of superior mechanical strength and elastin that is extremely durable and resilient [2, 4]. Notably, the silk-like blocks are capable of crystallizing to form virtual cross-links between elastin-mimetic sequences, which, in turn, lower the crystallinity of the silk-like blocks and thus enhance the solubility of SELPs. Consequently, SELPs may be fabricated into useful structures for biomedical applications, including drug delivery. In this study, we will characterize viscoelastic properties of SELPs, which are particularly relevant to tissue engineering applications.

scholarly journals Pain sensing neurons promote tissue regeneration in adult mice

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Lise Rabiller ◽  
Elodie Labit ◽  
Christophe Guissard ◽  
Silveric Gilardi ◽  
Bruno P. Guiard ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Tissue Regeneration ◽  
Tissue Repair ◽  
Animal Species ◽  
Loss Of Function ◽  
Nociceptive Neurons ◽  
Regulatory Processes ◽  
Adult Mice ◽  
Calcitonin Gene ◽  
Lower Vertebrates

AbstractTissue repair after injury in adult mammals, usually results in scarring and loss of function in contrast to lower vertebrates such as the newt and zebrafish that regenerate. Understanding the regulatory processes that guide the outcome of tissue repair is therefore a concerning challenge for regenerative medicine. In multiple regenerative animal species, the nerve dependence of regeneration is well established, but the nature of the innervation required for tissue regeneration remains largely undefined. Using our model of induced adipose tissue regeneration in adult mice, we demonstrate here that nociceptive nerves promote regeneration and their removal impairs tissue regeneration. We also show that blocking the receptor for the nociceptive neuropeptide calcitonin gene-related peptide (CGRP) inhibits regeneration, whereas CGRP administration induces regeneration. These findings reveal that peptidergic nociceptive neurons are required for adult mice tissue regeneration.

scholarly journals Microwave-Assisted Desulfation of the Hemolytic Saponins Extracted from Holothuria scabra Viscera

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 537
Author(s):  
Philippe Savarino ◽  
Emmanuel Colson ◽  
Guillaume Caulier ◽  
Igor Eeckhaut ◽  
Patrick Flammang ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Biological Properties ◽  
Chemical Defenses ◽  
Molecular Structures ◽  
Marine Animal ◽  
Holothuria Scabra ◽  
Saponin Content ◽  
Alkaline Conditions ◽  
Food Market ◽  
The Indian Ocean

Saponins are plant and marine animal specific metabolites that are commonly considered as molecular vectors for chemical defenses against unicellular and pluricellular organisms. Their toxicity is attributed to their membranolytic properties. Modifying the molecular structures of saponins by quantitative and selective chemical reactions is increasingly considered to tune the biological properties of these molecules (i) to prepare congeners with specific activities for biomedical applications and (ii) to afford experimental data related to their structure–activity relationship. In the present study, we focused on the sulfated saponins contained in the viscera of Holothuria scabra, a sea cucumber present in the Indian Ocean and abundantly consumed on the Asian food market. Using mass spectrometry, we first qualitatively and quantitatively assessed the saponin content within the viscera of H. scabra. We detected 26 sulfated saponins presenting 5 different elemental compositions. Microwave activation under alkaline conditions in aqueous solutions was developed and optimized to quantitatively and specifically induce the desulfation of the natural saponins, by a specific loss of H2SO4. By comparing the hemolytic activities of the natural and desulfated extracts, we clearly identified the sulfate function as highly responsible for the saponin toxicity.

Sign in / Sign up

Export Citation Format

Share Document