scholarly journals Deciphering Biochemical Responses, Metabolome Analysis and Key Genes Controlling Sorghum [Sorghum Bicolor (L.) Moench] Ion Transport in Responses to Salt Stress

2021 ◽  
Author(s):  
Himani Punia ◽  
Jayanti Tokas ◽  
Anurag Malik ◽  
Satpal ◽  
Neeraj Kharor ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stresses ◽  
Potential Model ◽  
Cellular Mechanism ◽  
Metabolome Analysis ◽  
Molecular Responses ◽  
Biochemical Responses ◽  
Key Genes ◽  
Sorghum Genotypes ◽  
Sorghum Bicolor L

Abstract Background: Crops usually encounter several abiotic stresses, and salt stress is one of the major constraints affecting plant growth and agricultural productivity globally. Sorghum is not only a valuable source of food but also a potential model for studying and better understanding the salt stress mechanics in the cereals and obtain a better knowledge of their cellular mechanism. Herein, we examined the effects of salt stress on physio-biochemical and molecular responses of sorghum genotypes to determine their tolerance.

scholarly journals GROWTH AND FOLIAR CONTENTS OF Na+ AND Cl- IN GENOTYPES OF FORAGE SORGHUM IRRIGATED WITH SALINIZED WATERS

Irriga ◽  
2018 ◽  
Vol 23 (1) ◽  
pp. 108-120 ◽  
Author(s):  
Daniela Siqueira Coelho ◽  
Welson Lima Simões ◽  
Alessandra Monteiro Salviano ◽  
Moisés Alves de Souza ◽  
Jair Andrade Lima ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Salt Stress ◽  
Sorghum Bicolor ◽  
Leaf Area ◽  
Growth And Yield ◽  
Forage Sorghum ◽  
Sorghum Genotypes ◽  
Foliar Concentration ◽  
E Mail ◽  
Sorghum Bicolor L

GROWTH AND FOLIAR CONTENTS OF Na+ AND Cl- IN GENOTYPES OF FORAGE SORGHUM IRRIGATED WITH SALINIZED WATERS  DANIELA SIQUEIRA COELHO1; WELSON LIMA SIMÕES2; ALESSANDRA MONTEIRO SALVIANO3; MOISÉS ALVES DE SOUZA4; JAIR ANDRADE LIMA5 E JOSÉ EZEQUIEL DE SANTOS6 1 Instituto do Meio Ambiente e Recursos Hídricos, rua A, s/n, 48906-652, Juazeiro - BA, e-mail: [email protected] Embrapa Semiárido, rodovia BR-428, Km 152, s/n, Zona Rural, 56302-970, Petrolina - PE, e-mail: [email protected] Embrapa Semiárido, rodovia BR-428, Km 152, s/n, Zona Rural, 56302-970, Petrolina - PE, e-mail: [email protected] Departamento de Biologia – Fisiologia Vegetal, Universidade Federal de Lavras, Câmpus Universitário, Caixa Postal 3037,  37200-000, Lavras - MG, e-mail: [email protected] Embrapa Semiárido, rodovia BR-428, Km 152, s/n, Zona Rural, 56302-970, Petrolina - PE, e-mail:  [email protected] Departamento de Biologia, Universidade de Pernambuco, rodovia BR 203, Km 2, s/n, 56328-903, Petrolina - PE,e-mail: [email protected]  1 ABSTRACT Plant efficiency in developing specific mechanisms to survive under salt stress can vary significantly among genotypes, which implies different responses in growth and yield. The aim of this study was to evaluate the salt tolerance of forage sorghum genotypes by analysis of growth variables correlated with the levels of Na+ and Cl- accumulated in the leaves. The research was carried out in a greenhouse belonging to the Brazilian Agricultural Research Corporation (EMBRAPA) – Semiarid, in Petrolina, Pernambuco state, Brazil. The treatments were arranged in a randomized block design with factorial arrangement 10x6 with ten genotypes (‘F305’, ‘BRS 655’, ‘BRS 610’, ‘Volumax’, ‘1,015,045’, ‘1,016,005’, ‘1,016,009’, ‘1,016,013’, ‘1,016,015’ and ‘1,016. 031’), salt solutions with six levels of electrical conductivity (0, 2.5, 5.0, 7.5, 10 and 12.5 dS m-1) and three replications. The variables evaluated were dry mass and water content of the shoots and roots, plant height, stern diameter, leaf area and foliar concentration of Na+ and Cl-. It was found that the growth of forage sorghum genotypes is similarly affected due to the increase of Na+ and Cl- foliar contents. The growth of forage sorghum is reduced by 50% when the plants are submitted to the application of saline solution with electrical conductivity of 8 dS m-1. Keywords: leaf area, salt stress, dry matter, Sorghum bicolor (L.) Moench.  COELHO, D. S.; SIMÕES, W. L.; SALVIANO, A. M.; SOUZA, M. A. de; LIMA, J. A.; SANTOS, J. E. deCRESCIMENTO E TEORES FOLIARES DE Na+ E Cl- EM GENÓTIPOS DE SORGO FORRAGEIRO IRRIGADOS COM ÁGUAS SALINIZADAS   2 RESUMO A eficiência das plantas em desenvolver mecanismos específicos para sobreviver ao estresse salino pode variar significativamente entre genótipos, implicando em respostas diferenciadas no seu crescimento e rendimento. O objetivo do presente estudo foi avaliar o crescimento e teores foliares de Na+ e Cl- em dez genótipos de sorgo forrageiro submetidos a irrigações com águas salinizadas. O experimento foi conduzido em casa de vegetação localizada na sede da Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) – Semiárido, em Petrolina – PE. Utilizou-se o delineamento experimental em blocos casualizados dispostos em esquema fatorial 10x6, considerando dez genótipos (‘F305’, ‘BRS 655’, ‘BRS 610’, ‘Volumax’, ‘1.015.045’, ‘1.016.005’, ‘1.016.009’, ‘1.016.013’, ‘1.016.015’ e ‘1.016.031’), soluções salinas com seis valores de condutividade elétrica (0; 2,5; 5,0; 7,5; 10 e 12,5 dS m-1) e três repetições.  As variáveis avaliadas foram massa seca e conteúdo de água da parte aérea e raízes, altura da planta, diâmetro do colmo, área foliar e teores foliares de Na+ e Cl-. Verificou-se que o crescimento dos genótipos de sorgo forrageiro foi afetado  similarmente com o aumento da salinidade devido à elevação das concentrações de Na+ e Cl- nas folhas. O crescimento do sorgo forrageiro é reduzido em 50% quando as plantas são submetidas à aplicação de solução salina com condutividade elétrica de 8 dS m-1. Palavras-chave: área foliar, salinidade, massa seca, Sorghum bicolor (L.) Moench. 

scholarly journals Ascorbate–Glutathione Oxidant Scavengers, Metabolome Analysis and Adaptation Mechanisms of Ion Exclusion in Sorghum under Salt Stress

2021 ◽  
Vol 22 (24) ◽  
pp. 13249
Author(s):  
Himani Punia ◽  
Jayanti Tokas ◽  
Anurag Malik ◽  
Andrzej Bajguz ◽  
Mohamed A. El-Sheikh ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Activities ◽  
Water Status ◽  
Membrane Integrity ◽  
Metabolome Analysis ◽  
Higher Plant ◽  
Ion Transporter ◽  
Cell Membrane Integrity ◽  
Ion Exclusion ◽  
Sorghum Genotypes

Salt stress is one of the major significant restrictions that hamper plant development and agriculture ecosystems worldwide. Novel climate-adapted cultivars and stress tolerance-enhancing molecules are increasingly appreciated to mitigate the detrimental impacts of adverse stressful conditions. Sorghum is a valuable source of food and a potential model for exploring and understanding salt stress dynamics in cereals and for gaining a better understanding of their physiological pathways. Herein, we evaluate the antioxidant scavengers, photosynthetic regulation, and molecular mechanism of ion exclusion transporters in sorghum genotypes under saline conditions. A pot experiment was conducted in two sorghum genotypes viz. SSG 59-3 and PC-5 in a climate-controlled greenhouse under different salt concentrations (60, 80, 100, and 120 mM NaCl). Salinity drastically affected the photosynthetic machinery by reducing the accumulation of chlorophyll pigments and carotenoids. SSG 59-3 alleviated the adverse effects of salinity by suppressing oxidative stress (H2O2) and stimulating enzymatic and non-enzymatic antioxidant activities (SOD, APX, CAT, POD, GR, GST, DHAR, MDHAR, GSH, ASC, proline, GB), as well as protecting cell membrane integrity (MDA, electrolyte leakage). Salinity also influenced Na+ ion efflux and maintained a lower cytosolic Na+/K+ ratio via the concomitant upregulation of SbSOS1, SbSOS2, and SbNHX-2 and SbV-Ppase-II ion transporter genes in sorghum genotypes. Overall, these results suggest that Na+ ions were retained and detoxified, and less stress impact was observed in mature and younger leaves. Based on the above, we deciphered that SSG 59-3 performed better by retaining higher plant water status, photosynthetic assimilates and antioxidant potential, and the upregulation of ion transporter genes and may be utilized in the development of resistant sorghum lines in saline regions.

scholarly journals Identification and Expression Profiling of Nonphosphorus Glycerolipid Synthase Genes in Response to Abiotic Stresses in Dendrobium catenatum

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1204
Author(s):  
Xinqiao Zhan ◽  
Yichun Qian ◽  
Bizeng Mao
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Expression Profiling ◽  
Abiotic Stresses ◽  
Membrane Lipids ◽  
Chinese Herb ◽  
Growth Stages ◽  
Limited Information ◽  
Gene Structures ◽  
Functional Investigation

Dendrobium catenatum, a valuable Chinese herb, frequently experiences abiotic stresses, such as cold and drought, under natural conditions. Nonphosphorus glycerolipid synthase (NGLS) genes are closely linked to the homeostasis of membrane lipids under abiotic stress in plants. However, there is limited information on NGLS genes in D. catenatum. In this study, a total of eight DcaNGLS genes were identified from the D. catenatum genome; these included three monogalactosyldiacylglycerol synthase (DcaMGD1, 2, 3) genes, two digalactosyldiacylglycerol synthase (DcaDGD1, 2) genes, and three sulfoquinovosyldiacylglycerol synthase (DcaSQD1, 2.1, 2.2) genes. The gene structures and conserved motifs in the DcaNGLSs showed a high conservation during their evolution. Gene expression profiling showed that the DcaNGLSs were highly expressed in specific tissues and during rapid growth stages. Furthermore, most DcaNGLSs were strongly induced by freezing and post-freezing recovery. DcaMGD1 and DcaSQDs were greatly induced by salt stress in leaves, while DcaDGDs were primarily induced by salt stress in roots. Under drought stress, most DcaNGLSs were regulated by circadian rhythms, and DcaSQD2 was closely associated with drought recovery. Transcriptome analysis also revealed that MYB might be regulated by circadian rhythm and co-expressed with DcaNGLSs under drought stress. These results provide insight for the further functional investigation of NGLS and the regulation of nonphosphorus glycerolipid biosynthesis in Dendrobium.

scholarly journals Jasmonates and Plant Salt Stress: Molecular Players, Physiological Effects, and Improving Tolerance by Using Genome-Associated Tools

2021 ◽  
Vol 22 (6) ◽  
pp. 3082
Author(s):  
Celia Delgado ◽  
Freddy Mora-Poblete ◽  
Sunny Ahmar ◽  
Jen-Tsung Chen ◽  
Carlos R. Figueroa
Keyword(s):  
Salt Stress ◽  
Abiotic Stresses ◽  
Synergistic Effects ◽  
Antioxidant Response ◽  
Crop Productivity ◽  
Point Of View ◽  
Physiological Effects ◽  
Crop Tolerance ◽  
Molecular Components ◽  
Ja Signaling

Soil salinity is one of the most limiting stresses for crop productivity and quality worldwide. In this sense, jasmonates (JAs) have emerged as phytohormones that play essential roles in mediating plant response to abiotic stresses, including salt stress. Here, we reviewed the mechanisms underlying the activation and response of the JA-biosynthesis and JA-signaling pathways under saline conditions in Arabidopsis and several crops. In this sense, molecular components of JA-signaling such as MYC2 transcription factor and JASMONATE ZIM-DOMAIN (JAZ) repressors are key players for the JA-associated response. Moreover, we review the antagonist and synergistic effects between JA and other hormones such as abscisic acid (ABA). From an applied point of view, several reports have shown that exogenous JA applications increase the antioxidant response in plants to alleviate salt stress. Finally, we discuss the latest advances in genomic techniques for the improvement of crop tolerance to salt stress with a focus on jasmonates.

Seed germination and biochemical responses of two Elytrigia elongata accessions exposed to abiotic stresses

2021 ◽  
Author(s):  
Xiaoxia Tian ◽  
Peichun Mao ◽  
Mingli Zheng ◽  
Qingyi Meng ◽  
Lin Meng
Keyword(s):  
Seed Germination ◽  
Abiotic Stresses ◽  
Biochemical Responses

scholarly journals Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 545
Author(s):  
Kumar Nishant Chourasia ◽  
Milan Kumar Lal ◽  
Rahul Kumar Tiwari ◽  
Devanshu Dev ◽  
Hemant Balasaheb Kardile ◽  
...  
Keyword(s):  
Salt Stress ◽  
Osmotic Stress ◽  
Salinity Stress ◽  
Crop Production ◽  
Antioxidant Activities ◽  
Water Status ◽  
Membrane Damage ◽  
Mitigation Strategies ◽  
Ammonium Compound ◽  
Molecular Responses

Among abiotic stresses, salinity is a major global threat to agriculture, causing severe damage to crop production and productivity. Potato (Solanum tuberosum) is regarded as a future food crop by FAO to ensure food security, which is severely affected by salinity. The growth of the potato plant is inhibited under salt stress due to osmotic stress-induced ion toxicity. Salinity-mediated osmotic stress leads to physiological changes in the plant, including nutrient imbalance, impairment in detoxifying reactive oxygen species (ROS), membrane damage, and reduced photosynthetic activities. Several physiological and biochemical phenomena, such as the maintenance of plant water status, transpiration, respiration, water use efficiency, hormonal balance, leaf area, germination, and antioxidants production are adversely affected. The ROS under salinity stress leads to the increased plasma membrane permeability and extravasations of substances, which causes water imbalance and plasmolysis. However, potato plants cope with salinity mediated oxidative stress conditions by enhancing both enzymatic and non-enzymatic antioxidant activities. The osmoprotectants, such as proline, polyols (sorbitol, mannitol, xylitol, lactitol, and maltitol), and quaternary ammonium compound (glycine betaine) are synthesized to overcome the adverse effect of salinity. The salinity response and tolerance include complex and multifaceted mechanisms that are controlled by multiple proteins and their interactions. This review aims to redraw the attention of researchers to explore the current physiological, biochemical and molecular responses and subsequently develop potential mitigation strategies against salt stress in potatoes.

scholarly journals Evaluation of Indigenous Olive Biocontrol Rhizobacteria as Protectants against Drought and Salt Stress

2021 ◽  
Vol 9 (6) ◽  
pp. 1209
Author(s):  
Nuria Montes-Osuna ◽  
Carmen Gómez-Lama Cabanás ◽  
Antonio Valverde-Corredor ◽  
Garikoitz Legarda ◽  
Pilar Prieto ◽  
...  
Keyword(s):  
Salt Stress ◽  
Abiotic Stresses ◽  
Biochemical Parameters ◽  
Biocontrol Agent ◽  
Negative Effects ◽  
Experimental Conditions ◽  
Acds Gene ◽  
Drought And Salt Stress ◽  
Physiological And Biochemical

Stress caused by drought and salinity may compromise growth and productivity of olive (Olea europaea L.) tree crops. Several studies have reported the use of beneficial rhizobacteria to alleviate symptoms produced by these stresses, which is attributed in some cases to the activity of 1-aminocyclopropane-1-carboxylic acid deaminase (ACD). A collection of beneficial olive rhizobacteria was in vitro screened for ACD activity. Pseudomonas sp. PICF6 displayed this phenotype and sequencing of its genome confirmed the presence of an acdS gene. In contrast, the well-known root endophyte and biocontrol agent Pseudomonas simiae PICF7 was defective in ACD activity, even though the presence of an ACD-coding gene was earlier predicted in its genome. In this study, an unidentified deaminase was confirmed instead. Greenhouse experiments with olive ‘Picual’ plants inoculated either with PICF6 or PICF7, or co-inoculated with both strains, and subjected to drought or salt stress were carried out. Several physiological and biochemical parameters increased in stressed plants (i.e., stomatal conductance and flavonoids content), regardless of whether or not they were previously bacterized. Results showed that neither PICF6 (ACD positive) nor PICF7 (ACD negative) lessened the negative effects caused by the abiotic stresses tested, at least under our experimental conditions.

scholarly journals Down-Regulation of SlGRAS10 in Tomato Confers Abiotic Stress Tolerance

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 623
Author(s):  
Sidra Habib ◽  
Yee Yee Lwin ◽  
Ning Li
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Abiotic Stress Tolerance ◽  
Functional Characterization ◽  
Flavonoid Biosynthesis ◽  
Ros Scavenging ◽  
Down Regulation

Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato SlGRAS10 gene under abiotic stresses such as salt stress and drought. Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O2− and H2O2. Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of SlGRAS10 as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant.

scholarly journals Identification of Key Genes in ‘Luang Pratahn’, Thai Salt-Tolerant Rice, Based on Time-Course Data and Weighted Co-expression Networks

2021 ◽  
Vol 12 ◽  
Author(s):  
Pajaree Sonsungsan ◽  
Pheerawat Chantanakool ◽  
Apichat Suratanee ◽  
Teerapong Buaboocha ◽  
Luca Comai ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Time Course ◽  
Rice Variety ◽  
Enrichment Analysis ◽  
Normal Growth ◽  
Growth Conditions ◽  
Pathway Enrichment Analysis ◽  
Key Genes

Salinity is an important environmental factor causing a negative effect on rice production. To prevent salinity effects on rice yields, genetic diversity concerning salt tolerance must be evaluated. In this study, we investigated the salinity responses of rice (Oryza sativa) to determine the critical genes. The transcriptomes of ‘Luang Pratahn’ rice, a local Thai rice variety with high salt tolerance, were used as a model for analyzing and identifying the key genes responsible for salt-stress tolerance. Based on 3' Tag-Seq data from the time course of salt-stress treatment, weighted gene co-expression network analysis was used to identify key genes in gene modules. We obtained 1,386 significantly differentially expressed genes in eight modules. Among them, six modules indicated a significant correlation within 6, 12, or 48h after salt stress. Functional and pathway enrichment analysis was performed on the co-expressed genes of interesting modules to reveal which genes were mainly enriched within important functions for salt-stress responses. To identify the key genes in salt-stress responses, we considered the two-state co-expression networks, normal growth conditions, and salt stress to investigate which genes were less important in a normal situation but gained more impact under stress. We identified key genes for the response to biotic and abiotic stimuli and tolerance to salt stress. Thus, these novel genes may play important roles in salinity tolerance and serve as potential biomarkers to improve salt tolerance cultivars.

scholarly journals Thymol improves salinity tolerance of tobacco by increasing the Na+ efflux and enhancing the content of NO and GSH

2021 ◽  
Author(s):  
liang xu ◽  
Jia-Qian Song ◽  
yuelin wang ◽  
Xiao-Han Liu ◽  
Xue-Li Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Root Growth ◽  
Abiotic Stresses ◽  
Nature Medicine ◽  
Oxygen Species ◽  
Plant Tolerance ◽  
Ros Accumulation ◽  
Na Efflux ◽  
The Stability

Abstract Plants have evolved a lot of strategies to improve salt tolerance to cope with salt stress. Recent studies have suggested that thymol (a nature medicine) enhances the plant tolerance against abiotic stresses, but the mechanisms are rarely known. Here, we found that thymol played an important role in maintaining root growth under salt stress. Thymol rescued root growth from salt stress via ameliorating ROS (reactive oxygen species) accumulation, lipid peroxidation, and cell death. In addition, thymol enhanced the level of NO (nitric oxide) and GSH (glutathione) to repress ROS accumulation, further protecting the stability of cell membrane. Thymol-induced Na+ efflux in roots and leaves under salt stress may depend on the upregulation of SOS1, HKT1 and NHX1. Consequently, all of these evidences suggested that thymol improved tobacco salt tolerance via enhancing NO and GSH content as well as inducing Na+ efflux.

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