Morphological and functional criteria for the effectiveness of recreational activities in children

Objective. The study aimed to perform a comprehensive morphofunctional assessment of the effectiveness of recreational activities in children aged 9–12 years in the summer of 2019.Materials and methods. The study was conducted by the “Problem scientific laboratory of physical methods of diagnosis and treatment” of RostGMU in the children’s health and recreation camp “Mir” (Krasnyi Desant village, Gulf of Taganrog). Procedures and time of the study: somatometry, bioimpedance analysis, сardiointervalography, stabilometrics on the 2nd day of stay and 2.5 weeks after. Two groups were formed: Group I included overweight children (OW), n = 15 (boys, n = 9; girls, n = 6); Group II included children with normal physical development (NPD), n = 37 (boys, n = 17; girls, n = 20). The children received a non-drug complex of camp resource provision for 3 weeks.Results. Health measures did not lead to significant changes in the somatometric parameters. According to the results of the сardiointerval recording, an authentic increase in the variational range and vegetative rhythm index was found in children with NPD, which indicates an increase in the parasympathetic activity of regulation. The stress index decreased by 30% in the case of NPD, and by 6% in the case of OW. Data from the stabilometric “Balls” simulator showed that after the recovery, the regulation of postural control was optimized, and decision-making processes were accelerated, especially in children with NPD.Conclusion. The study showed that in order to assess the effectiveness of recreational activities, along with “mandatory” methods, it is advisable to use functional methods such as cardiointervalography and stabilometrics, which can be recommended for use in health and rehabilitation institutions to assess the adaptive capabilities of the organism.

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Protective effects of tempol in an experimental ovarian ischemia–reperfusion injury model in female Wistar albino rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2016-0309 ◽

2017 ◽

Vol 95 (7) ◽

pp. 861-865 ◽

Cited By ~ 9

Author(s):

Neslihan Pınar ◽

Oya Soylu Karapınar ◽

Oğuzhan Özcan ◽

Esin Atik Doğan ◽

Suphi Bayraktar

Keyword(s):

Sham Group ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Stress Index ◽

Albino Rats ◽

Protective Effects ◽

Group V ◽

Group Iii ◽

Group I ◽

Wistar Albino Rats

The aim of this study was to investigate the antioxidant effects of tempol on ovarian ischemia–reperfusion (I/R) injury in rats. Forty female Wistar albino rats were randomly divided into 5 groups: Group I, sham; Group II, ischemia (I); Group III, I/R; Group IV, I/R + tempol 30 mg/kg i.p; Group V, I/R + tempol 50 mg/kg i.p. Oxidative stress index (OSI) was significantly higher in the ischemia group and the I/R group than in the sham group. Catalase levels were significantly lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. Glutathione peroxidase levels were lower in the I/R group than in the I/R + tempol 30 mg/kg i.p. and the I/R + tempol 50 mg/kg i.p. groups. MDA levels were significantly lower in the I/R + tempol 30 mg/kg i.p. group and the I/R + tempol 50 mg/kg i.p. group than in the I/R group. The levels of the histopathological parameters were significantly decreased in the I/R + tempol 50 mg/kg i.p. group compared with the I/R group. Tempol can be used for reducing ovarian I/R injury.

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Effects of Flunixin Meglumine on Oxidant and Antioxidant System after Disbudding with Caustic Paste in Calves

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.88542 ◽

2018 ◽

Vol 46 (1) ◽

pp. 9

Author(s):

Selvinaz Yakan ◽

Vesile Duzguner ◽

Ozgur Aksoy

Keyword(s):

Oxidative Stress ◽

Local Anesthesia ◽

Antioxidant System ◽

Live Weight ◽

Stress Index ◽

Blood Samples ◽

Flunixin Meglumine ◽

Group I ◽

Group Ii ◽

Commercial Kit

Background: Disbudding is often practiced in modern farm because of the reasons for the safekeeping of the animal, other animals lessen the risk of injury and less aggressive behavior. Without regard to the method of disbudding leads to disruption behavioral changes, cardiac and endocrine responses related pain of animals. Sedation, local anesthesia and analgesia are performed studies in order to eliminate of cardiac, endocrine, behavioral response which is caused by pain associated with disbudding. In this study, it was aimed to determine the effect on the oxidant and antioxidant system in the calves of disbudding by using the caustic paste with and without painkiller.Materials, Methods & Results: The animal material of the study was created 24 Simmentals calves in different sexes on average 2 weeks (± 2 days) and 50 kg (± 15 kg) live weight. The cases were randomly divided into 2 groups of 12 calves in each group. In the group I (analgesia group); caustic paste+painkiller (2.2 mg-1 flunixin meglumine intravenous, IV) and group II (non-analgesia group); as disbudding applied with caustic pasta application only were divided into 2 groups. In the I group, 15th min before the application, flunixin meglumine at a dose of 2.2 mg-1 was administered IV and the horn blunting was performed by caustic paste method. Blood samples were taken at 15, 30 and 60th min after completion of horn blunting and physiological findings were recorded. In group II, the horns of the calves were disbudding with the same technique (but no analgesia aplication). Blood samples were taken at 15, 30 and 60th min after completion of horn blunting and physiological findings were record. Cortisol, glucose, total oxidant capacity (TOC) and total antioxidant capacity (TAC), 8-hydroxy-2’-deoxyguanosine (8-OHdG), glutathione (GSH), superoxide dismutase (SOD) enzymes measurements (ELISA) were performed in both groups. TOC and TAC tests were used to determine the oxidative stress index in the obtained serum, 8-OHdG measurement which is the most sensitive and most frequent oxidative DNA damage indicator for determination of oxidative damage; GSH, SOD enzymes were analyzed by ELISA using commercial kit for the evaluation of antioxidant potential. For evaluation of pain in animals, cortisol (ELISA) and glucose level in serum were measured with ELISA by using commercial kit. In the group without flunixin meglumine, cortisol was increased significantly from the 15th min (P < 0.05), but there was no increase in the 30 and 60th min in painkiller administered group. Glucose levels were higher in the group not using painkillers than those used at 15th (P < 0.001) and 30, 60th min (P < 0.05). TOC (P < 0.001) and 8-OHdG (P < 0.05) values were significantly higher in the group without painkiller while TAC, GSH and SOD levels (P < 0.05) were significantly decreased.Discussion: It has been observed that sedation (such as xylazine) and local anesthesia (such as lidocaine) use removed pain-induced stress in the disbudding with caustic paste in calves. However, in order to remove the endocrine response, it is necessary to combine the anesthesia protocol with the analgesic (such as flunixin meglumine) combination. According to our results concluded that the painkiller given before the disbudding process was supporting the antioxidant system, reducing the level of cortisol and oxidative stress.

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CLINICAL COURSE AND CONTROL OF BRONCHIAL ASTHMA INDICATORS IN OVERWEIGHT SCHOOLCHILDREN

Clinical & experimental pathology ◽

10.24061/1727-4338.xx.1.75.2021.4 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

L.A. Ivanova ◽

M.N. Haras ◽

I.B. Horbatiuk ◽

A.O. Shkilnyuk

Keyword(s):

Body Weight ◽

Bronchial Asthma ◽

The Body ◽

Excess Body Weight ◽

Clinical Group ◽

Overweight Children ◽

Group I ◽

Negative Effect ◽

And Control ◽

Bodymass Index

The aim of the study - to study the clinical features and indicators for asthma control inoverweight children to optimize treatment strategies.Material and methods. 200 schoolchildren with asthma who were treated in the pulmoallergy department of the Chernivtsi Regional Children's Clinical Hospital have beenexamined. 52 patients with excess body weight (body mass index was greater than 25,0)belonged to group I, and children with body weight corresponding to the age norm (bodymass index from 18,0 to 24,9) were included into clinical group II under observation.Results. It has been found that the average number of points according to the ASTquestionnaire in patients of group I reached 14,0 ± 1,33 points, and in the comparisongroup – 16,3 ± 0,54 points (p˃0,05). At the same time, the share of patients withuncontrolled course of the disease among the overweight children reached 61,5% incomparison to 48,3% in the comparison group (p˃0,05).Indices of the risk of uncontrolled asthma (the sum of AST test scores <16) in childrenwith overweight have shown the following results: odds ratio = 1,6 (95% CI: 0,29-8,59),relative risk = 1,4 95% CI: 1,00-2,08) and attributive risk = 6,8%. Before treatment theindex of AKDNFG - 2,4 dinitrophenylhydrazones (AKDNFG) of the main character in thegroup of excess body weight children was 60,8 mmol /g of protein, and in group of normalbody weight children – 59,6 ± 9 mmol /g of protein. After the course of basic therapy,these indices gave next results – 47,2 ± 4,18 mmol/g of protein in group I and 4,3 ± 0,29mmol /g of protein (p> 0,05) in group II.Conclusions. Bronchial asthma in overweight children is more likely to debut at an earlyage and characterized by a persistent course. Predisposition to the body overweight inpatients with bronchial asthma has a negative effect on the indices of the disease controlachievement, although it is accompanied by an evidence decrease of the inflammatoryprocess of the respiratory tract in the course of treatment.

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Candesartan in a rat model of testicular toxicity: New insight on its protective mechanism

Experimental Biology and Medicine ◽

10.1177/1535370219842149 ◽

2019 ◽

Vol 244 (7) ◽

pp. 593-601 ◽

Cited By ~ 1

Author(s):

Iman O Sherif ◽

Osama M Sarhan

Keyword(s):

Protective Effect ◽

Mrna Level ◽

Stress Index ◽

Protective Mechanism ◽

Angiotensin Ii Receptor Blocker ◽

Testicular Toxicity ◽

Angiotensin Ii Receptor ◽

Histopathological Findings ◽

Group I ◽

Anti Inflammatory

Cisplatin (CDDP) is widely used as an effective chemotherapy; nevertheless, its use is associated with male reproductive system damage. Candesartan (Cand) is an angiotensin II receptor blocker which showed a protective effect against CDDP-induced testicular toxicity. This study was implemented to investigate further novel molecular protective effect of Cand. Animals were divided into four groups and treated for 10 days as: Group I (Normal control): received saline, Group II (Cand control): treated with Cand (10 mg/kg/day) orally, Group III (CDDP): injected with a single dose of 10 mg/kg CDDP intraperitoneally (ip), Group IV (Cand + CDDP): treated with Cand (10 mg/kg/day) orally plus a single ip dose of 10 mg/kg CDDP at day 3. Blood and testicular tissue collections were done at the end of the experiment. A marked decrease in testicular, body, and relative body weights in addition to testosterone level was observed in the CDDP group when compared with normal rats. In addition, exposure to CDDP showed a marked upregulation of testicular TNF-α mRNA level and a significant rise in testicular levels of total oxidant status, oxidative stress index (OSI) ratio, pro-apoptotic protein Bax, Bax/Bcl-2 ratio alongside with a marked reduction in testicular levels of total antioxidant status, antiapoptotic protein Bcl-2, and a significant upregulation of the testicular caspase-3 expression in comparison to normal group. Histopathological findings after CDDP injection showed apoptosis and necrosis in testicular tissues. Administration of Cand ameliorated these biochemical and histopathological findings. Cand exhibited a novel protective mechanism against CDDP induced-gonadal damage via antioxidant, anti-inflammatory, and anti-apoptotic activities. Impact statement Cisplatin is a commonly used drug in the treatment of solid tumors and its application is associated with testicular toxicity. The effect of candesartan in cisplatin-induced testicular toxicity and its fundamental mechanism of action were investigated. Candesartan had certainly repaired the testicular injury and ameliorated both biochemical and histopathological changes. Candesartan mitigated the gonadotoxicity induced by cisplatin via antioxidative, anti-inflammatory, and antiapoptotic actions.

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Protective Effects of Caffeic Acid Phenethyl Ester on Fluoxetine-Induced Hepatotoxicity: An Experimental Study

BioMed Research International ◽

10.1155/2016/1247191 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Ahmet Yılmaz ◽

Bilal Elbey ◽

Ümit Can Yazgan ◽

Ahmet Dönder ◽

Necmi Arslan ◽

...

Keyword(s):

Caffeic Acid ◽

Histopathological Examination ◽

Caffeic Acid Phenethyl Ester ◽

Group Iv ◽

Paraoxonase 1 ◽

Stress Index ◽

Control Group ◽

Group Iii ◽

Group I ◽

Liver Tissues

Background. The aim of the study was to analyse the effect of caffeic acid phenethyl ester (CAPE) on fluoxetine-induced hepatotoxicity in rats.Materials and Methods. Group I served as control. Group II received CAPE intraperitoneally. Group III received fluoxetine per orally. Group IV received fluoxetine and CAPE. The total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), and liver enzymes including paraoxonase-1 (PON-1), aspartate transaminase, and alanine transaminase levels were measured. Liver tissues were processed histopathologically for evaluation of liver injury and to validate the serum enzyme levels.Results. An increase in TOS and OSI and a decrease in TAC and PON-1 levels in serum and liver tissues of Group III were observed compared to Groups I and II. After treatment with CAPE, the level of TOS and OSI decreased while TAC and PON-1 increased in serum and liver in Group IV. Histopathological examination of the liver revealed hepatic injury after fluoxetine treatment and reduction of injury with CAPE treatment.Conclusion. Our results suggested that CAPE treatment provided protection against fluoxetine toxicity. Following CAPE treatment with fluoxetine-induced hepatotoxicity, TOS and OSI levels decreased, whereas PON-1 and TAC increased in the serum and liver.

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Effect of intrauterine infusion of Momordica charantia L. on oxidative stress and pregnancy rate in infertile cows

Journal of Veterinary Research ◽

10.1515/jvetres-2017-0063 ◽

2017 ◽

Vol 61 (4) ◽

pp. 489-496 ◽

Cited By ~ 1

Author(s):

Birten Emre ◽

Ömer Korkmaz ◽

Füsun Temamoğulları ◽

Abuzer Kafar Zonturlu ◽

İsmail Koyuncu ◽

...

Keyword(s):

Oxidative Stress ◽

Olive Oil ◽

Pregnancy Rate ◽

Lipid Hydroperoxide ◽

Momordica Charantia ◽

Stress Index ◽

Inflammatory Status ◽

Group I ◽

Significant Difference ◽

Subclinical Endometritis

AbstractIntroduction: The effect of intrauterine administration of Momordica charantia L. (MC) extract on oxidative changes and pregnancy rate in infertile cows was investigated.Material and Methods: Endometrial smear specimens were taken from 40 cows with fertility problems for cytological examination, and the cows were randomly divided into two groups: group I (n = 20) was subjected to intrauterine administration of 40 mL (0.25 g/mL) of MC extract, group II (n = 20) was subjected to intrauterine administration of 40 mL of pure olive oil. Blood samples were taken starting from the day of administration of MC extract or olive oil (day 0) and then for three weeks at weekly intervals (days 7, 14, 21). Blood serum samples were evaluated for total antioxidant capacity (TAS), total oxidant level (TOS), oxidative stress index (OSI), lipid hydroperoxide (LOOH), and nitric oxide (NO) levels. In addition, on the 14th day following treatment, two doses of PGF2α were administrated to all cows at 14-day intervals. Following the second PGF2α administration, insemination and GnRH administration was performed at the 60th h after PGF2α treatment. Smear samples were stained with Giemsa and immunohistochemically to determine cytological changes and inflammatory status.Results: According to cytological findings, subclinical endometritis was a prevalent disorder in cows with infertility problem (82.5%; 33/40). Additionally, 60.6% (20/33) of the cows with subclinical endometritis had acute inflammation, whereas remaining 13 cows had chronic endometritis. Of the cows with subclinical endometritis, 50% (8/16) and 35% (6/17) became pregnant in group I and II, respectively (P > 0.05). Although the oxidative stress parameters showed similarities between both groups (P > 0.05), there was a significant difference (P < 0.001) between the groups in terms of mean NO and LOOH levels (NO – 31.20 ± 11.38 vs 44.53 ± 11.50 μmol/L and LOOH – 1.22 ± 0.37 vs 1.89 ± 0.36 μmol/L).Conclusion: The obtained results indicated that MC administration, especially in the presence of active inflammation, may improve the pregnancy rate by positive reduction of oxidative changes.

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EFFECT OF INTERNSHIP ON THE ADAPTIVE POSSIBILITIES OF TURNER TRAINEES

Kuban Scientific Medical Bulletin ◽

10.25207/1608-6228-2019-26-1-108-113 ◽

2019 ◽

Vol 26 (1) ◽

pp. 108-113 ◽

Cited By ~ 1

Author(s):

Ol’ga V. Kiyok

Keyword(s):

Heart Rate ◽

Heart Rate Variability ◽

Conflict Of Interest ◽

Environmental Conditions ◽

Nervous Activity ◽

Stress Index ◽

Adaptive Capabilities ◽

Higher Nervous Activity ◽

Genetically Determined

Aim. The present work assesses the effect of internship on the adaptive capabilities of turner trainees according to the dynamics of the regulatory and adaptive status.Materials and methods. In 30 turner trainees at the age of 18, internship adaptation was studied according to the dynamics of the index of the regulatory and adaptive status (IRAS) and heart rate variability. IRAS was determined using the sample of cardio-respiratory synchronism. The use of IRAS allows the regulatory-adaptive capabilities to be assessed quantitatively, classifying them as “high” (with an index of the regulatory-adaptive status >100), “good” (50–99), “satisfactory” (25–49), “low” (10–24) and “unsatisfactory” (≤ 9). In accordance with the Strelau temperament inventory, genetically determined types of higher nervous activity determine the ability of an organism to adapt to changing environmental conditions. Following this notion, the trainees were divided into the groups of less adapted (melancholic, choleric, sanguine/choleric, melancholic/choleric) and more adapted (phlegmatic, sanguine, phlegmatic/ sanguine, phlegmatic/melancholic) individuals.Results.The groups of more adapted and less adapted individuals showed a decrease in IRAS by 24.4% and 38.8%, respectively. Accordingly, the regulatory and adaptive capabilities of the organism in the more adapted group remained “good”, while in the less adapted group they became “satisfactory”. Variation pulsometry indicated an increase in the stress index at the end of the internship, especially for less adapted turner trainees.Conclusion.The dynamics of regulatory and adaptive capabilities allows the adaptation to internship among trainees to be assessed.Conflict of interest: the author declares no conflict of interest.

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Carvacrol prevents methotrexate-induced renal oxidative injury and renal damage in rats

Clinical & Investigative Medicine ◽

10.25011/cim.v37i1.20865 ◽

2014 ◽

Vol 37 (1) ◽

pp. 19 ◽

Cited By ~ 13

Author(s):

Mehtap Bozkurt ◽

Serda Em ◽

Pelin Oktayoglu ◽

Gul Turkcu ◽

Hatice Yuksel ◽

...

Keyword(s):

Single Dose ◽

Renal Damage ◽

Statistical Significance ◽

Kidney Tissue ◽

Male Rats ◽

Control Treatment ◽

Stress Index ◽

Control Group ◽

Group Iii ◽

Group I

Purpose: The purpose of this study was to investigate the effect of carvacrol (CAR) on methotrexate (MTX)-induced renal damage in rats. Methods: Twenty-four male rats were equally divided into three groups: group I, control treatment; group II, MTX-treated; and group III, MTX+CAR-treated. A single dose of CAR (73 mg/kg) was administered intraperitoneally to group III on the first day of the experiment and a single dose of MTX (20 mg/kg) was administered intraperitoneally to groups II and III on the second day of the experiment. Blood samples and kidney tissue were obtained from each animal on day 8 for the measurement of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI). Light microscopy was used for histopathological examination of kidney specimens. Results: MDA, TOS and OSI levels were significantly greater in the group receiving MTX alone relative to the control animals, while the TAS level was significantly reduced in the MTX group compared with the control group. The administration of CAR was associated with significantly decreased MDA, TOS, and OSI levels and increased TAS levels relative to the rats treated with MTX alone. Animals treated with CAR exhibited decreased tubular degeneration and architectural impairment relative to animals treated with MTX alone; however, the difference in histological scores did not meet the threshold of statistical significance. Conclusions: MTX treatment results in oxidative damage to the rat kidney; damage which is partially abrogated by the administration of CAR.

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Oxidative and Antioxidative Status of Children with Celiac Disease Treated with a Gluten Free-Diet

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/1324820 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Grażyna Rowicka ◽

Grażyna Czaja-Bulsa ◽

Magdalena Chełchowska ◽

Agnieszka Riahi ◽

Małgorzata Strucińska ◽

...

Keyword(s):

Oxidative Stress ◽

Celiac Disease ◽

Density Lipoprotein ◽

Gluten Free Diet ◽

Stress Index ◽

Healthy Children ◽

Gluten Free ◽

Strict Adherence ◽

Antioxidative Status ◽

Group I

Aims. Oxidative stress is a factor involved in the pathogenesis of celiac disease (CD), possibly affecting the course of the disease and celiac-related complications. We assessed the intensity of oxidative processes and the efficiency of antioxidant defense in children with celiac disease. Methods. Group I (n=32) consisted of children with CD treated with a gluten-free diet, and group II (n=24) consisted of healthy children on a traditional diet. Antioxidative and oxidative status was assessed by measurement of serum total antioxidant capacity (TAC), total oxidant capacity (TOC), and oxidized low-density lipoprotein (ox-LDL) and on the basis of oxidative stress index (OSI).Results. There were no significant differences in serum TAC, TOC, ox-LDL, and OSI between children with CD and healthy children. Cluster analysis showed that the group of children with CD is not homogeneous in terms of serum TAC and TOC levels. About 50% of these children had TAC levels < 1.3 mmol/L and TOC levels > 0.35 mmol/L.Conclusions. Strict adherence to a gluten-free diet by children with CD seems to be important for maintaining oxidative-antioxidant balance. However, further research is needed to identify factors potentially responsible for increased oxidative stress in some children with celiac disease despite adherence to a gluten-free diet.

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Decision-making for receiving paid home care for dementia in the time of COVID-19: a qualitative study

BMC Geriatrics ◽

10.1186/s12877-020-01719-0 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Clarissa Giebel ◽

Kerry Hanna ◽

Jacqueline Cannon ◽

Ruth Eley ◽

Hilary Tetlow ◽

...

Keyword(s):

Public Health ◽

Decision Making ◽

Home Care ◽

Home Care Services ◽

Health Measures ◽

Care Services ◽

Decision Making Processes ◽

The Uk ◽

The Impact ◽

Care Support

Abstract Background The lockdown imposed in the UK on the 23rd of March and associated public health measures of social distancing are likely to have had a great impact on care provision. The aim of this study was to explore the decision-making processes of continued paid home care support for dementia in the time of COVID-19. Methods Unpaid carers caring for a person living with dementia (PLWD) who were accessing paid home care before COVID-19 and residing in the UK were eligible to take part. Participants were interviewed over the phone and asked about their experiences of using paid home care services before and since COVID-19, and their decision-making processes of accessing paid home care since the outbreak and public health restrictions. Results Fifteen unpaid carers, who were also accessing paid care support for the PLWD before COVID-19, were included in the analysis. Thematic analysis identified three overarching themes: (1) Risk; (2) Making difficult choices and risk management; and (3) Implications for unpaid carers. Many unpaid carers decided to discontinue paid carers entering the home due to the risk of infection, resulting in unpaid carers having to pick up the care hours to support the person living with dementia. Conclusions This is the first study to report on the impact of COVID-19 on paid home care changes in dementia. Findings raise implications for providing better Personal Protective Equipment for paid carers, and to support unpaid carers better in their roles, with the pandemic likely to stay in place for the foreseeable future.

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