scholarly journals PENGARUH EKSTRAK DAUN LIDAH BUAYA (Aloe vera L.) TERHADAP PERTUMBUHAN JAMUR Candida albicans SECARA IN VITRO

2017 ◽  
Vol 5 (1) ◽  
pp. 72
Author(s):  
Feizia Huslina
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Aloe Vera ◽  
Pathogenic Fungi ◽  
Gastrointestinal Infections ◽  
Extract Concentration ◽  
Randomized Design ◽  
Significant Difference ◽  
Variant Analysis

The purpose of this study was to determine the ability of aloe vera leaf extract (Aloe vera L.) in inhibiting the growth of Candida albicans fungi through in vitro. Aloe vera (Aloe vera L.) is a plant that can overcome digestive problems caused by fungi. One of the pathogenic fungi causing gastrointestinal infections is C. albicans. This research was conducted in Microbiology Laboratory of FMIPAUnsyiah which lasted from April to May 2010. A Completely Randomized Design (RAL) consisted of 5 treatments and 5 replications is used in this study. The treatment was arranged on P0 = 20 μl distilled water, P1 = aloe vera leaf extract concentration 100%, P2 = aloe leaf extract concentration of 50% and P3 = aloe leaf extract concentration of 25%. Aloe vera leaf extract (Aloe vera L.) was given to each treatment at 20 μl. The parameter observed was the diameter of the inhibit zone formed. The data were analyzed using Variant Analysis (ANOVA), then continued with the Smallest Significant Difference (BNT) test. The results of this study indicated that the presence of aloe vera leaf extract (Aloe vera L.) could inhibit the growth of C. albicans. The higher the concentration of aloe vera leaf extract (Aloe vera L.), the higher the ability of it to inhibit C. albicans. The diameter of the inhibitory zone formed will enlarge along with the increasing of the concentration of aloe vera leaf extract (Aloe vera L.). The extract concentrations of 100%, 50% and 25% had their resistance capability of 0.50 mg, 0.24 mg and 0.20 mg of nistatin antibiotics.

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

scholarly journals Antifungal Activity of Beluntas “Indian Camphorweed” (Pluchea indica) Ethanol Extract on Candida albicans In Vitro Using Different Solvent Concentrations

2021 ◽  
Author(s):  
Wayan Larissa Demolsky ◽  
Vinna Kurniawati Sugiaman ◽  
Natallia Pranata
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Pathogenic Fungi ◽  
Inhibitory Effect ◽  
Plate Count ◽  
Minimum Fungicidal Concentration ◽  
Significant Difference

Abstract Objective Oral candidiasis is an infection caused by pathogenic fungi Candida albicans, with a considerably high prevalence of 20 to 72%. Indian camphorweed (Pluchea indica) also known as “beluntas” as the local name has been known as a traditional medicine in Indonesia. The objective of this study is to research the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of beluntas ethanolic extract against the growth of C. albicans. Materials and Methods The MIC and MFC were measured by microdilution assay and total plate count respectively with a variation of solvents (DMSO 1%, 10%, and 4%) and beluntas extract with concentrations between 0.3125 and 200 mg/mL. Amphotericin and nystatin were used as a comparison. Statistical Analysis One-way analysis of variance and posthoc Tukey test were used to determine the significant difference between treatments. Results It was found that the MIC ranged from 50 to 200 mg/mL in the test with DMSO 10% solvent and MFC was found to be at a concentration of 200 mg/mL. However, there is a significant inhibitory effect and killing effect from DMSO 10% against C. albicans (p = 0.000). MIC was also found within concentrations of 100 mg/mL of beluntas extract in DMSO 4%. In this study, the DMSO 4% concentration neither showed significant inhibitory effects nor killing effects; therefore, the result was acceptable (p = 0.357). Conclusion Ethanol extract of beluntas (P. Indica) has the potential of being an antifungal agent with inhibitory activity in concentrations ≥100 mg/mL, which is similar to nystatin (p = 0.278). The MFC for the extract was above 100 mg/mL, which cannot be measured with this method as a higher concentration of DMSO is needed, which had a toxic effect on the tested fungi.

scholarly journals Efektivitas Antifungi Ekstrak Daun Patikan Kebo (Euphorbia hirta) Terhadap Jamur Penyebab Kandidiasis (Candida albicans)

2019 ◽  
Vol 2 (1) ◽  
pp. 22
Author(s):  
Zulkarnain Zulkarnain ◽  
Cut Muthiadin ◽  
Fatmawati Nur ◽  
Rusmadi Rukmana
Keyword(s):  
Infectious Disease ◽  
Candida Albicans ◽  
Leaf Extract ◽  
Microbial Growth ◽  
Inhibition Zone ◽  
Euphorbia Hirta ◽  
Randomized Design ◽  
Paper Disk ◽  
Significant Difference ◽  
Completely Randomized Design

<em>Candidiasis is an infectious disease caused by the fungus Candida albicans. This  study aims to determine the effect of patikan kebo (Euphorbia hirta) leaf extract in inhibiting the growth of C. albicans. This research is an experimental study which was compiled using a Completely Randomized Design (CRD) by giving Patikan Kebo (E. hirta) leaf extract  in several concentrations, the concentrations of 40%, 45%, 50%, 55%, and 60% and controls (aquadest) and C. albicans mushroom with three repetitions. The parameters measured are the amount of inhibitory diameter formed around the paper discs in the 24 hour and 48 hour period. D ata obtained with three repetitions and observations of 24 hours and 48 hours showed that giving patikan kebo leaf extract in several types of concentration had an effect on inhibiting the growth and fungi of C . albicans. Furthermore, the data obtained were analyzed using analysis of variance (F-test) at the level of α 0.05 and continued using the Smallest Significant Difference (LSD) α 0.05 where the results showed that the giving of patikan kebo extract significantly affected the microbial growth test (C. albicans), where the higher the concentration of patikan kebo leaf extract, the greater the inhibition zone formed around the paper disk. </em>

scholarly journals Effectiveness Test of Soursop Leaf Extract and Papaya Leaf Extract Combination Against Spodoptera litura on Chili Plants in Tobelo City

2020 ◽  
Vol 13 (2) ◽  
pp. 253
Author(s):  
Nonice Manikome ◽  
Morina Handayani
Keyword(s):  
Mortality Rate ◽  
Leaf Extract ◽  
Morphological Changes ◽  
Chemical Compounds ◽  
The Body ◽  
Extract Concentration ◽  
Randomized Design ◽  
Initial Symptoms ◽  
Significant Difference ◽  
Completely Randomized Design

In terms of control, various natural chemical compounds derived from plants can be used. Like papaya and soursop leaves, the formulation of the problem is whether the combination of soursop leaf extract and papaya leaf extract is effective in controlling S. litura pests and what is the most effective extract concentration. This study used a completely randomized design (RBD) with 5 treatments and 5 replications. Observation variables included symptoms, morphological changes and mortality, the results were analyzed by Analysis of Variance (ANOVA) followed by the Least Significant Difference (LSD) 5% test. The initial symptoms that were seen one day after application of the combination were morphological changes in the test larvae. Such as damage to the body of the larva, change in color, then the larva's body shrinks, when touched the larvae easily fall. The highest mortality was found at the extract concentration of 20% with a mortality rate of 64.79% on the second day. The results showed that the high mortality rate was closely related to the high toxicity contained in the 20% extract concentration. It was concluded that the results of the effectiveness test of the combination of papaya leaf extract and soursop leaf extract were effective. Suggestions for the use of natural materials that are easily available can be used as an alternative to control pests that are environmentally friendly. Furthermore, it is suggested that if you want to increase mortality, you can try increasing the extract concentration.

scholarly journals Aktivitas Antijamur Air Perasan Daun Seledri (Apium graveolens L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 4 (2) ◽  
pp. 102
Author(s):  
Patra Inova Ardelia ◽  
Fauzia Andrini ◽  
M Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Fungicidal Activity ◽  
Negative Control ◽  
Apium Graveolens ◽  
Diffusion Method ◽  
Randomized Design ◽  
Significant Difference ◽  
Post Hoc

Celery (Apium graveolens L.) has been known as traditional medicine. Apigenin is one of active component in Celeryleaf that has fungicidal activity, so celery leaf’s extortion suspected has fungicidal activity to Candida albicans. Apigenincan solved in water. The aim of this research was to know antifungal activity of celery leaf’s extortion againsts Candidaalbicans in vitro.This is a laboratory experimental research by using completely randomized design and disc diffusion method. Celeryleaf’s extortion was divided into 3 concentrations, there were 25%, 50% and 100%. Ketoconazole 2% was used aspositive control and aquadest as negative control. The result of this research was based on analysis of variance, therewas significant difference between minimal 2 treatment (p=0.000) and confirmed with Post Hoc test, there were significantdifference in all treatments. As the conclusion, celery leaf’s extortion had antifungal activity againsts Candida albicansin vitro with the best concentration 50% but smaller than ketoconazole 2%.

scholarly journals The Potency of Anting Anting (Acalypha indica L.) Leaf Extract as An Acaricide on Boophilus microplus in Larvae and Adult Stages In Vitro

2021 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Fitri Nurdiana ◽  
Rahmi Sugiharuti ◽  
Bambang Sektiari Lukiswanto
Keyword(s):  
Larval Stage ◽  
Leaf Extract ◽  
Tween 80 ◽  
Boophilus Microplus ◽  
Factorial Anova ◽  
Larval Stages ◽  
Randomized Design ◽  
Significant Difference ◽  
Acalypha Indica

The purpose of this study was to determine the potency of anting-anting leaf extract (Acalypha indica L.) as an acaricide against larval and adult Boophilus microplus in vitro by observing the mortality rate of B. microplus. The present study design was a completely randomized design. This study used five treatments, including: Tween 80 1% and aquadest (K-), Neguvon (K+), anting-anting leaf extract with a concentration of 3.125% (P1), 6.25% (P2), and 12.5% (P3 ). Each treatment used 5 repetitions and each repetition used 6 B. microplus larval stages and 5 B. microplus adults. Observations were made for 5 hours for the larval stage of B. microplus and 24 hours for the adult stage of B. microplus. Boophilus microplus is declared dead if there is no movement at all when touched with a needle. The data obtained were analyzed using factorial ANOVA and continued with Duncan's multiple distance test. The results showed that the 12.5% anting anting leaf extract treatment showed no significant difference with the treatment using Neguvon. (p>0.05) in larval stage B. microplus, but in adult B. microplus showed a significant difference with Neguvon (p<0.05). The results of the statistical test can be concluded that the extract of anting-anting leaf (A. indica L.) has the potential as an acaricide of B. microplus larval stage in vitro. The higher the concentration of anting anting leaf extract, the higher the acaricide activity.

scholarly journals Perbandingan Daya Hambat Larutan Antiseptik Povidone iodine dengan Ekstrak Daun Sirih terhadap Candida albicans secara In Vitro

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Septriana Putri ◽  
Aziz Djamal ◽  
Rahmatini Rahmatini ◽  
Cimi Ilmiawati
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Vaginal Discharge ◽  
Povidone Iodine ◽  
Inhibitory Effect ◽  
Iodine Solution ◽  
Betel Leaf ◽  
Post Hoc ◽  
Povidone Iodine Solution

Abstrak Candida albicansb (C. albicans) adalah salah satu mikroorganisme penyebab masalah kesehatan reproduksi wanita, yaitu keputihan (fluor albus). Penggunaan larutan povidone iodine dan bahan alam seperti ekstrak daun sirih menjadi pilihan masyarakat sebagai pembersih alat kewanitaan. Tujuan penelitian ini adalah untuk membandingkandaya hambat larutan antiseptik povidone iodine dan ekstrak daun sirih terhadap jamur C. albicans secara in vitro. Penelitian dilakukan terhadap lima isolat jamur C. albicans dengan larutan kontrol akuades.Perlakuan terdiri dari povidone iodine, ekstrak daun sirih dengan konsentrasi 5%, 10%, dan 20%.Hasil penelitian menunjukkan bahwa povidone iodine memiliki daya hambat terhadap C. albicans. Ekstrak daun sirih dengan konsentrasi 5% dan 10% tidak memiliki daya hambat terhadap C. albicans, namun ekstrak daun sirih konsentrasi 20% memiliki daya hambat terhadap C. albicans. Analisis statistik dengan uji ANOVA yang dilanjutkan dengan uji Post-hoc menunjukkan perbedaan bermakna antara daya hambat larutan povidone iodine dan ekstrak daun sirih 20% terhadap kontrol(p < 0.05).Larutan povidone iodine memiliki daya hambat dua kali lebih besar terhadap pertumbuhan C. albicans dibandingkan ekstrak daun sirih 20%. Dari penelitian ini dapat disimpulkan bahwa larutan povidone iodine dan ekstrak daun sirih 20% dapat menghambat pertumbuhan jamur C. albicans secara in vitro. Kata kunci: povidone iodine, ekstrak daun sirih, Candida albicansAbstract Candida albicans (C. albicans) is one of the frequent causes of  reproductive health problems in women, namely vaginal discharge (fluor albus). The antiseptic solution, povidone iodine, is still an option to overcome vaginal discharge. The use of natural materials such as betel (Piper betle L.) leaves extract also become a popular choice as adouche for women. The objective of this study was to compare the inhibitory activity of povidone iodine solution and betel leaf extract against the growth of C. albicans in vitro. We used five different isolates of C. albicans with distilled water as control. Each isolate was treated with povidone iodine solution, betel leaf extract at concentration of 5%, 10%,and 20%. The results showed that povidone iodine had inhibitory effect on C. albicans. Betel leaf extract at concentration of 5% and 10% did not have inhibitory effectwhile betel leaf extract at concentration of 20% hadinhibitory effect on C. albicans. Analysis by ANOVA followed by Post-hoc tests showed a significant difference in inhibitory activity of povidone iodine and betel leaf extract at 20% concentration compared to control (p < 0,05). Povidone iodine solution showed twice as much as inhibitory effect on C. albicans compared to betel leaf extract (20% concentration). It is concluded that povidone iodine solution and betel leaf extract at 20% concentration can inhibit the growth of C. albicans in vitro.Keywords: povidone iodine, betel leaf extract, Candida albicans

scholarly journals KECERNAAN IN VITRO SILASE SAMPAH SAYUR DAN DAUN GAMAL MENGGUNAKAN MIKROORGANISME RUMEN KAMBING

2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Dry Matter ◽  
Vegetable Waste ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.

scholarly journals Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tamirat Bekele Beressa ◽  
Serawit Deyno ◽  
Paul E. Alele
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Cryptococcus Neoformans ◽  
Antioxidant Properties ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

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