scholarly journals HIPPOCAMPUS CELL DISORDERS AND NEUROSENSORY TESTS IN MICE (Mus musculus) DUE TO INDUCTION OF EXCESS SODIUM CHLORIDE

2021 ◽  
Vol 7 (2) ◽  
pp. 97-100
Author(s):  
Putri Cahyaningrum ◽  
Yuanita Windusari ◽  
Arum Setiawan

Salt or sodium chloride (NaCl) is an additive to give food a salty taste. The use of salt in everyday life is difficult to avoid. Salt has both good and bad effects on the body. The electrolyte content of salt can help launch metabolism in the body, whereas if the body contains too much salt it will cause heart attacks and hypertension. The dose of salt consumption that has been set by the government is 5g/day. This study was conducted to examine the effect of consuming excessive sodium chloride (NaCl) on the ability to smell and to what extent it damages cells in the hippocampus of mice (Mus musculus). The method of this study was CRD (completely randomized design) with 6 replications, 1 group control and 3 repetitions induced by sodium chloride (NaCl) is 260mg/gBB (P1), 520mg/gBB (P2), and 780mg/gBB (P3). The parameters of this study are neurosensory coordination in the form of olfactory response of mice (Mus musculus) to ammonia and cell disruption in the hippocampus (DG & CA) which were observed by histological preparations of Hematoxylin eosin (HE) staining. The results of this study indicate the presence of olfactory disorders in mice (Mus musculus) and cell death in the hippocampus also increased due to excessive sodium chloride (NaCl) induction. The more salt is consumed in daily life, it will disrupt the cells in the hippocampus.

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4575
Author(s):  
Julyana Machado da Silva Martins ◽  
Evandro De Abreu Fernandes ◽  
João Paulo Rodrigues Bueno ◽  
Carolina Magalhães Caires Carvalho ◽  
Fernanda Heloisa Litz ◽  
...  

<p>The objective of this study was to evaluate the effect of different nutritional plans on the body temperature and organ biometrics in male and female broilers, of two ages. Here, 1,700 birds were used (850 males and 850 females) in a completely randomized design composed of five treatments (- 3%, - 1.5%, reference, + 1.5% and + 3%), with 10 repetitions, totaling 50 experimental units; the reference treatment based on nutritional and energy levels indicated in previous studies was calculated from this. At 35 and 42 d, the temperatures of the wing, head, shin, back, and cloaca in males and females were measured separately, and the average surface and body temperature were calculated. At 42 d, relative weights of the gizzard, liver, heart, and small intestine were calculated. The temperatures of the wings, back, and cloaca, and consequently the average surface temperature and body temperatures, were not affected by nutritional plans. Effects of increasing the nutritional and energy levels were observed on liver weights, the gizzard, and the small intestine. We conclude that the nutritional plans did not affect body temperature. Males had higher body temperatures than females. Body temperature increased with increase in age, and the increase in the nutritional plans increased liver weight and reduced the gizzard weights.</p>


2019 ◽  
Vol 20 (1) ◽  
pp. 1-5
Author(s):  
Wuye Ria Andayanie

Black grass jelly plants can be used as conservation plants. However, uret pests (Leucopholis rorida F.) are often complained that eat the base of the stems, flowers and roots at night. This study aims to determine: 1) the effect of M. anisopliae fungi on uret pests, (2) the level of susceptibility of uret pests, and (3) M. anisopliae fungal effectiveness on uret pests. This study used a completely randomized design (CRD) with 4 treatments and 5 replications. Data from the observations were analyzed using analysis of variance (ANOVA) with Duncan's Multiple Range Test (DMRT) 5% test carried out when diversity analysis showed a real effect. Uret mortality monitoring will be carried out after the application of fungi. by observing each uret and calculating the amount of uret mortality and average plant growth. The highest percentage of second instar larvae from uret was found in treatment P4 (manure and fungi were incubated for 72 hours at the next temperature of 28 oC applied to the field with straw mulch) which was 82, 98% at 12 days after application of fungi. The average growth of plants consisting of: root length, root volume, stem diameter and number of shoots was also found in treatment P4 (manure and fungi were incubated for 72 hours at the next 28 oC, applied to the field with straw mulch), respectively amounting to 12.45; 5.70; 1.88; 12,15. Dead larvae emit fluids such as ethanol and their bodies are weak, then the body dries and hardens and stiffens, like mummies and fungi forming green sporulation. Keywords—: black grass jelly plants; Leucopholis rorida F;Metharhizium anisopliae; mortality;effectiveness


el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>


2010 ◽  
Vol 10 (2) ◽  
pp. 21-26
Author(s):  
Sugito Sugito ◽  
Erdiansyah Rahmi ◽  
Azhari Azhari ◽  
M. Isa

The gain body weight and Spoilage of meat broiler which giving jaloh extract to combine with chromiumABSTRACT. A study was conducted on broiler chickens under heat stress to evaluate the effect of combined jaloh (Salix tetrasperma Roxb) extract with chromium mineral in the body weight, value of feed ratio conversion, and time to meat to become decomposed. Twenty four of 20-day old Cobb female broiler chickens were randomly assigned and divided to 4 treatment groups. Completely randomized design was used in this study. The treatments were as follows: 1) chickens given no heat stress and jaloh extract nor chromium (tCp); 2) chickens under heat stress without given jaloh extract nor chromium (Cp); 3) chickens under heat stress and given jaloh extract in 1.000 mg/lt drinking water (Cp+Ej); and 4) chickens under heat stress, given jaloh extract in 1.000 mg/lt drinking water, and chromium in 1.000 ug/lt drinking water (Cp+Ej+Cr). Heat stress given was 33 ± 1oC of cage temperature during 4 hours per day within 15 days. Jaloh extract and chromium treatments in drinking water were given at 2 hour before cage temperature reaching 33 ± 1oC and were stopped being given after 1 hour, when cage temperature back to room temperature. On the 16th day of the study chickens were measured before slaughtered. Samples obtained were chicken meat that taken from breast part (musculus pectoralis). The result suggested that either giving jaloh extract per se at the dose of 1.000 mg/lt in drinking water or combine it with chromium that given two hours before cage temperature reaching 33 ± 1oC, can prevent chickens from decreasing their body weight, decreasing value of feed ratio convertion, and extending time to meat to become decomposed.


2021 ◽  
Vol 4 (1) ◽  
pp. 41-45
Author(s):  
Umbang Arif Rokhayati ◽  
Soegeng Herijanto

The purpose of this study was to determine the organoleptic value of salty taste in eggs soaked in salt solution with different soaking times. The materials used in this study were 90 eggs, rubbing ash and crust salt (table salt). The experimental design used was a completely randomized design (CRD) with 3 treatments and 3 replications. The parameters observed in this study were the organoleptic value and salinity level. The results showed that the duration of soaking eggs in a salt solution had a significant effect on the organoleptic value and salinity of the eggs (P 0.05) on the organoleptic value and the salinity level of the panelists preferred the results of soaking in a salt water solution with a long soaking time of 12 days.


2020 ◽  
Vol 21 (2) ◽  
pp. 124-130
Author(s):  
Mhd Zalil Efendi ◽  
Ramadhan Sumarmin ◽  
M. Syukri Fadil

Torch ginger has a wide range of good antimicrobial, antioxidant, anticancer, larvicidal and repellent activities. Active compounds in Torch ginger that affect pharmacological activities are phenols, polyphenols, flavonoids, and terpenoids. Based on these ingredients Torch ginger can be used to heal wounds. This study aims to observe the effect of Torch ginger Umbut extract on wound healing in mice. This study hopes to add information about the effect of Torch ginger umbut extract on the healing of cuts in mice so that it can be another alternative for wound healing and can be a reference for other researchers. This study used a completely randomized design with 5 treatments and 3 replications. Tests carried out on adult male mice. The results showed that the optimal wound healing in P2 is treatment with 10% Torch ginger umbut extract which requires a range of wound healing 7-8 days. Based on these results, Torch ginger umbut extract can heal wounds.


2020 ◽  
Vol 50 (5) ◽  
Author(s):  
Kethylleen de Carvalho Ferreira ◽  
Aline Correia Furtado ◽  
Hugo Pereira Flores ◽  
Pollyanna Ricartes de Oliveira de Oliveira ◽  
Augusto Galhardo Gonçalves ◽  
...  

ABSTRACT: This study sought to evaluate the number of bruises on bovine carcasses and their relationship with loading rates in different truck models. Bruising percentages in the hindquarter, forequarter and short rib regions were evaluated. The space occupied on the truck by each animal in m² was defined as the Practiced area, obtained by dividing the body area by the number of males and females transported in straight trucks (10.60 x 2.40 m) and livestock trailers (14.80 x 2.60 m), 240 and 168, and 120 and 93, respectively, and 80 males in a straight truck with trailer configuration (17.50 x 2.60 m). The minimum area occupied by the animals was assessed according to the Farm Animal Welfare Council (FAWC) and Animal Welfare Advisory Committee (AWAC). The data was analyzed in a completely randomized design and included two sex classes, three carcass regions and three truck types. For males, the minimum areas (m²) calculated by the FAWC and AWAC were smaller (1.37 and 1.29 m², respectively) for the straight truck. The straight truck with trailer configuration had the lowest (P=0.0025) bruising index in the forequarter region (15.1%) and the highest (P=0.047) in the short rib region (30.5%). Females transported in the livestock trailer had a higher (P<0.001) percentage of bruises in the forequarter region (51.7%). There was a relationship between the high bruising rates and the loading rate Practiced for the males. Estimations made by the AWAC are close to those practiced in the upper Pantanal region of Mato Grosso do Sul, Brazil.


Author(s):  
Warobi Warobi ◽  
Delima Engga Maretha ◽  
Asnilawati Asnilawati ◽  
Mashuri Masri

Air fresheners are products that contain chemicals aimed at reducing unpleasant odours in confined spaces. The use of synthetic air fresheners turns out to harm health because some of their leased Volatile Organic Compounds are classified as toxic compounds and are carcinogens. Air freshener enters the body through the inhalation process in the respiratory system. Modern air fresheners are available in liquid (aerosol) and gel forms. Air fresheners contain addictive substances and solvents such as 1,4-dichlorobenzene which can affect pulmonary function. The purpose of this study is to know the effect of exposure to liquid air freshener on the histology of bronchi of mice (Mus musculus). This study used 20 male mice (Mus musculus) consisting of 4 treatments with 5 replications. The research design is true experimental in the form of a post-test only control group with Completely Randomized Design (CRD). The post-test was done by observing the histological picture of the mice's bronchi after exposure to liquid air freshener given 3x/day for a period of P1 = 2 weeks, P2 = 4 weeks and P3 = 6 weeks. Quantitative data on bronchial histology was tested using the One Way ANOVA statistical test followed by the Post Hoc Tukey test. The results of the study found changes in the histology of the bronchi, thickening of the epithelial tissue of mice. Analysis of comparative data between the control and treatment groups statistically obtained p = 0.010 <0.05, meaning that there was a significant effect and change in the bronchial tubes exposed to liquid air freshener. Histologically there were differences in histology between the control and treatment groups. It was concluded that liquid air freshener had a significant effect on the histological picture of the mice's bronchi.


2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4575
Author(s):  
Julyana Machado da Silva Martins ◽  
Evandro De Abreu Fernandes ◽  
João Paulo Rodrigues Bueno ◽  
Carolina Magalhães Caires Carvalho ◽  
Fernanda Heloisa Litz ◽  
...  

The objective of this study was to evaluate the effect of different nutritional plans on the body temperature and organ biometrics in male and female broilers, of two ages. Here, 1,700 birds were used (850 males and 850 females) in a completely randomized design composed of five treatments (- 3%, - 1.5%, reference, + 1.5% and + 3%), with 10 repetitions, totaling 50 experimental units; the reference treatment based on nutritional and energy levels indicated in previous studies was calculated from this. At 35 and 42 d, the temperatures of the wing, head, shin, back, and cloaca in males and females were measured separately, and the average surface and body temperature were calculated. At 42 d, relative weights of the gizzard, liver, heart, and small intestine were calculated. The temperatures of the wings, back, and cloaca, and consequently the average surface temperature and body temperatures, were not affected by nutritional plans. Effects of increasing the nutritional and energy levels were observed on liver weights, the gizzard, and the small intestine. We conclude that the nutritional plans did not affect body temperature. Males had higher body temperatures than females. Body temperature increased with increase in age, and the increase in the nutritional plans increased liver weight and reduced the gizzard weights.


2020 ◽  
Vol 41 (3) ◽  
pp. 945
Author(s):  
Román Maza Ortega ◽  
Mário Fonseca Paulino ◽  
Edenio Detmann ◽  
Luciana Navajas Rennó ◽  
Deilen Sotelo Moreno ◽  
...  

The objective in this study was to evaluate the effects of supplementation levels on productive and nutritional performance and metabolic profile in suckling female calves under grazing. Forty female calves (averaging 3.5±0.06 months and 127.3±2.68 kg), and their respective dams were distributed in a completely randomized design with two treatments and twenty replicates. The treatments were 1) 4 g kg-1 body weight (BW) of supplement or 2) 6 g kg-1 BW of supplement. Forage and organic matter (OM) intake did not affect (P > 0.05) by levels of supplement, though crude protein and non-fibrous carbohydrates intake were greater (P < 0.05) by increasing supplementation level. There was no effect (P > 0.05) the supplementation levels on OM and CP digestibility. The metabolic profile of the animals was not affected (P > 0.05) by supplementation level. Average daily gain, longissimus dorsi area, fat thickness over rump of the animals did not affect (P > 0.05) by levels of supplement. However, there was trend of increasing (P=0.074) in fat thickness over loin by increase the supplementation level. Although the body growth of animals was similar (P > 0.05) between treatments, there was observed a trend of increase (P=0.064) in ratio BW:Height at the withers by increasing supplementation levels. In conclusion, increasing the supplementation level of 4 to 6 g kg-1 of BW, not improve the productive and nutritional performance and metabolic status in female calves under grazing on creep-feeding system.


Sign in / Sign up

Export Citation Format

Share Document