scholarly journals Assessing the impact of purslane (Portulaca oleracea L.) on growth performance, anti-oxidative, and immune activities in grass carp (Ctenopharyngodon idella)

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ehsan Ahmadifar ◽  
Seyed Hossein Hoseinifar ◽  
Hosein Adineh ◽  
Mohsen Shahriari Moghadam ◽  
Mahmoud A.O. Dawood
Keyword(s):  
Growth Performance ◽  
Lipase Activity ◽  
Grass Carp ◽  
Ethanolic Extract ◽  
Portulaca Oleracea ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Immunoglobulin Level ◽  
T1 And T2 ◽  
The Impact

AbstractIn this study, the basal diet was supplemented with ethanolic extract of purslane (Portulaca oleracea L.) and the possible effects on growth performance, anti-oxidative, and immune activities of grass carp were evaluated. Fish with initial weight 1.23 ± 0.11g were randomly divided into four groups (triplicates) and fed purslane extract at 0% (T0), 0.5% (T1), 1% (T2), and 1.5% (T3) for 56 days. At the end of the feeding trial, the results showed that growth parameters were enhanced in T1 groups compared to the control group (P<0.05). Lipase activity in T1 and T2 groups increased, whereas no significant changes were noticed in cases of amylase and protease activities (P>0.05). Catalase and superoxide dismutase activities were enhanced in all groups fed the supplemented diets in comparison with the control group (P<0.05). However, no significant alteration was noticed in the case of glutathione peroxidase activity following the administration of purslane extract (P>0.05). A significant increase in total immunoglobulin level was noted in the T1 group, but lysozyme activity was higher in T1 and T2 groups compared to the control group (P<0.05). In conclusion, supplementation of grass carp diet with the purslane ethanolic extract, especially at 0.5%, can improve growth performance, lipase activity, the antioxidant enzyme activities as well as the immune response of grass carp fingerlings.

scholarly journals Effect of Probiotics (Bacillus subtilis) on the Growth and Survival of Fingerlings of Grass Carp, Ctenopharyngodon idella

2021 ◽  
pp. 31-37
Author(s):  
Shahziya Shah ◽  
Anayitullah Chesti ◽  
Mansoor Rather ◽  
Mehak Hafeez ◽  
Anam Aijaz ◽  
...  
Keyword(s):  
Growth Rate ◽  
Bacillus Subtilis ◽  
Growth Performance ◽  
Grass Carp ◽  
Water Quality Management ◽  
Ctenopharyngodon Idella ◽  
Control Group ◽  
Efficiency Ratio ◽  
Grass Carp Ctenopharyngodon Idella ◽  
The Impact

In the modern high intensity aquaculture, probiotics offer an encouraging substitute to chemicals and antibiotics, one such important application of probiotics is their use as growth promoters, in addition to health and water quality management. On the same background, the study was carried to evaluate the effect of dietary incorporation of probiotic - Bacillus subtilis on the growth performance of grass carp (Ctenopharyngodon idella).The probiotic - Bacillus subtilis was mixed with the basal diet (Protein 32%) in three different concentrations (0.5, 1.0 and 1.5 % designated as T1, T2, and T3). The basal feed with no probiotic was used as control (T0). The impact was recorded for a period of 60 days. Feeding was done twice a day at the rate of 5% of their body weight. Growth performance was evaluated through estimation of weight gain, feed Conversion Ratio (FCR), protein Efficiency Ratio (PER), specific growth rate (SGR) and feed efficiency ratio (FER). It was observed that the probiotic Bacillussubtilis fed at 1.5% significantly improved the growth performance of the fish Ctenopharyngodon idella showing the highest growth rate followed by 1% and 0.5% probiotic fed diets. Lowest growth rate was recorded in control group.

scholarly journals Effects of Dietary Paper Mulberry (Broussonetia Papyrifera) on Growth Performance and Muscle Quality of Grass Carp (Ctenopharyngodon idella)

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1655
Author(s):  
Tao Tang ◽  
Jinhai Bai ◽  
Zhipeng Ao ◽  
Zehong Wei ◽  
Yi Hu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Muscle Fiber ◽  
Grass Carp ◽  
Crude Protein ◽  
Fiber Diameter ◽  
Control Diet ◽  
Ctenopharyngodon Idella ◽  
Muscle Quality ◽  
Control Group ◽  
Broussonetia Papyrifera

The present study investigated the effects of dietary paper mulberry (Broussonetia Papyrifera, BP) on growth performance, muscle quality and muscle growth-related mRNA expressions of grass carp. Fish (initial weight: 50.0 ± 0.5 g) were fed diets supplemented with 0% (control diet), 5%, 10%, 15% and 20% BP for 8 weeks. The results showed that increasing levels of paper mulberry linearly and quadratically decreased the special gain rate (SGR) and increased the feed conversion rate (FCR) of grass carp (p < 0.05). Significantly positive quadratic trends were found between paper mulberry levels and muscle crude fat or crude protein of grass carp (p < 0.05). In comparison to the control diet, the 10%BP and 15%BP groups had significantly decreased muscle crude fat and increased crude protein (p < 0.05). The levels of paper mulberry resulted in a linear and quadratic increase in water loss of grass carp muscle (p < 0.05), and all groups with paper mulberry supplementation were significantly higher than the control group (p < 0.05). Significant positive linear and quadratic trends were found between the paper mulberry levels and muscle fiber diameter or density of grass carp (p < 0.05). In comparison to the control diet, the significant differences were found in the 15%BP and 20%BP groups (p < 0.05). The muscle adhesiveness and hardness linearly and quadratically increased with the increasing levels of paper mulberry (p < 0.05), and both of which increased significantly when the level of paper mulberry reached 10% (p < 0.05). In addition, the increase in paper mulberry linearly and quadratically improved the expressions of myoblast determination protein (MyoD), myogenin (MyoG), paired box protein 7 (Pax7) and myostatin 1 (MSTN1) (p < 0.05). When the supplementation of paper mulberry reached 15%, the expressions of all these mRNAs were significantly higher than those of the control group (p < 0.05). In summary, adding 5% paper mulberry did not affect the growth of grass carp. However, the supplementation of 10% paper mulberry could improve muscle quality through improving muscle hardness, reducing fat accumulation and muscle fiber diameter, at the cost of reducing growth performance.

scholarly journals 14. Effect of Dietary Supplementation of Garlic (Allium Sativum) and Turmeric (Curcuma Longa) on Growth Performance, Carcass Traits, Blood Profile and Oxidative Status in Growing Rabbits

2016 ◽  
Vol 16 (2) ◽  
pp. 489-505 ◽  
Author(s):  
Mahmoud Alagawany ◽  
Elwy Ali Ashour ◽  
Fayez Mohamed Reda
Keyword(s):  
Growth Performance ◽  
Control Diet ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Rabbit Serum ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Growth Promoter ◽  
Blood Profile

Abstract This study was performed to investigate the effects of dietary supplementation with garlic and turmeric powder as growth promoter agents on performance, carcass traits, serum biochemistry, and antioxidant enzyme activities of growing rabbits. A total of 112 New Zealand White rabbits (NZW) at 5 weeks of age were randomly assigned to seven treatments with four replicates. The dietary treatments consisted of 7 groups as follows; the basal diet as control, phytogenic additives groups were supplemented with 2, 4, and 6 g/kg garlic or turmeric powder added to the basal diet. There were no linear and quadratic differences (P<0.05) in growth performance after garlic or turmeric supplementation at all studied ages. Compared with the control group, supplementation of diets with garlic or turmeric linearly and quadratically elevated immunity biomarkers such as total protein (TP), albumin (AL) and immunoglobulin (IgG) levels and decreased (linearly and quadratically, P<0.05) aspartate aminotransferase (AST), alanine aminotransferase (ALT ), triglyceride (TG) and total cholesterol (TC) levels in rabbit serum. However, TP, AL, globulin (GL), IgG and IgM levels were linearly and quadratically enhanced with increasing turmeric levels versus the control diet. Hepatic superoxide dismutase (SOD), catalase (CAT ) and glutathione peroxidase GSH-Px activities as well as reduced glutathione (GSH) concentrations were linearly and quadratically (P<0.05) improved in garlic or turmeric additives fed groups. While MDA concentration was statistically (linearly, P= 0.022) reduced in comparison with the control group. It could be concluded that garlic or turmeric supplementation (2, 4 and 6 g/kg) did not linearly and quadratically affect growth performance but improved the immunity responses and lowered the lipid profile in blood and lipid peroxidation in liver and increased hepatic antioxidant activity in treated rabbits.

scholarly journals Effects of dietary dihydropyridine supplementation on growth performance and lipid metabolism of broiler chickens

2010 ◽  
Vol 55 (No. 3) ◽  
pp. 116-122 ◽  
Author(s):  
Z.Y. Niu ◽  
F.Z. Liu ◽  
Y.N. Min ◽  
W.C. Li
Keyword(s):  
Lipid Metabolism ◽  
Growth Performance ◽  
Lipase Activity ◽  
Feed Efficiency ◽  
Apolipoprotein B ◽  
Density Lipoprotein ◽  
Abdominal Fat ◽  
Lipoprotein Cholesterol ◽  
Optimum Dose ◽  
Control Group

An experiment was conducted to investigate the effects of dihydropyridine supplementation on growth performance and lipid metabolism of broilers. A total of 480 one-day-old female Arbor Acres broiler chicks were randomly divided into four groups, each group had six replications of 20 birds. Each group was fed a maize-soybean meal diet supplemented with 0, 100, 200, 300 mg/kg dihydropyridine, respectively, for six weeks. At 42 days of age, body weight and feed intake were not affected by dihydropyridine, while feed efficiency was significantly increased by 8.4%, 15.0% and 12.0%, respectively (<I>P </I>&lt; 0.05). The percentage of abdominal fat and the percentage of liver fat were reduced by 24.5%, 25.9%, 23.3%, and 23.6%, 26.7%, 26.0%, respectively (<I>P </I>&lt; 0.05). The higher level of dietary dihydropyridine (200 or 300 mg/kg) increased the hormone-sensitive triglyceride lipase activity in liver and abdominal fat (<I>P </I>&lt; 0.05). The lipoprotein lipase activity in abdominal fat was significantly decreased by dihydropyridine (<I>P </I>&lt; 0.05). The activity of glucose-6-phosphate dehydrogenase and malic dehydrogenase in liver was significantly reduced, whereas the isocitrate dehydrogenase activity in liver was not affected by dietary dihydropyridine. The content of cAMP was significantly increased by dihydropyridine, but malondialdehyde content was decreased (<I>P </I>&lt;&nbsp;0.05). Dihydropyridine at doses of 100 and 200 mg/kg increased apolipoprotein B (<I>P </I>&lt; 0.05), but 300 mg/kg dihydropyridine had no effect on apolipoprotein B compared with the control group. Triiodothyronine was significantly increased by dietary dihydropyridine (<I>P </I>&lt; 0.05). There were no differences in apolipoprotein A, cholesterol, trigly-cerides, high density lipoprotein-cholesterol, very low density lipoprotein-cholesterol, thyroxine and insulin among dietary treatments. It is concluded that supplementing dihydropyridine has a beneficial effect on feed efficiency and lipid metabolism of broilers, and that 200 mg/kg dihydropyridine supplementation is the optimum dose.

scholarly journals Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1031 ◽  
Author(s):  
Mohamed M. Abdel-Daim ◽  
Samah R. Khalil ◽  
Ashraf Awad ◽  
Ehsan H. Abu Zeid ◽  
Reda Abd El-Aziz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathway ◽  
Moringa Oleifera ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Kidney Tissue ◽  
Ethanolic Extract ◽  
Male Rats ◽  
Control Group ◽  
The Impact

This study aimed to describe the protective efficacy of Moringa oleifera ethanolic extract (MOEE) against the impact of cobalt chloride (CoCl2) exposure on the rat’s kidney. Fifty male rats were assigned to five equal groups: a control group, a MOEE-administered group (400 mg/kg body weight (bw), daily via gastric tube), a CoCl2-intoxicated group (300 mg/L, daily in drinking water), a protective group, and a therapeutic co-administered group that received MOEE prior to or following and concurrently with CoCl2, respectively. The antioxidant status indices (superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)), oxidative stress markers (hydrogen peroxide (H2O2), 8-hydroxy-2-deoxyguanosine (8-OHdG), and malondialdehyde (MDA)), and inflammatory response markers (nitric oxide (NO), tumor necrosis factor (TNF-α), myeloperoxidase (MPO), and C-reactive protein (CRP)) were evaluated. The expression profiles of pro-inflammatory cytokines (nuclear factor-kappa B (NF-kB) and interleukin-6 (IL-6)) were also measured by real-time quantitative polymerase chain reaction (qRT-PCR). The results showed that CoCl2 exposure was associated with significant elevations of oxidative stress and inflammatory indices with reductions in the endogenous tissue antioxidants’ concentrations. Moreover, CoCl2 enhanced the activity of the NF-κB inflammatory-signaling pathway that plays a role in the associated inflammation of the kidney. MOEE ameliorated CoCl2-induced renal oxidative damage and inflammatory injury with the suppression of the mRNA expression pattern of pro-inflammatory cytokine-encoding genes. MOEE is more effective when it is administered with CoCl2 exposure as a prophylactic regimen. In conclusion, MOEE administration exhibited protective effects in counteracting CoCl2-induced renal injury in rats.

scholarly journals Use of Chemical Nano-Selenium as an Antibacterial and Antifungal Agent in Quail Diets and Its Effect on Growth, Carcasses, Antioxidant, Immunity and Caecal Microbes

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3027
Author(s):  
Mahmoud Alagawany ◽  
Shaza Y. A. Qattan ◽  
Youssef A. Attia ◽  
Mohamed T. El-Saadony ◽  
Shaaban S. Elnesr ◽  
...  
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Growth Performance ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Bacterial Count ◽  
Japanese Quails ◽  
Intestinal Pathogens ◽  
The Impact

Nano-minerals are used to enhance mineral bioavailability, which helps improve animal growth and health. The use of chemical nano-selenium (Che-SeNPs) has lately attracted great scientific interest, mainly due to its potential benefits for poultry. The current study was conducted to investigate the impact of the dietary supplementation of Che-SeNPs on the growth performance, carcass traits, blood constituents, antioxidant status, immunity, and gut microbiota of Japanese quails. A total of one week-old 180 Japanese quails were randomly distributed into four equal groups, and each group consisted of 45 unsexed birds with five replications (nine birds each). The first group was fed a basal diet without supplementation (0 g/kg Che-SeNPs), and the second, third, and fourth groups were fed diets containing 0.2, 0.4, and 0.6 g/kg Che-SeNPs, respectively. The results showed that the dietary supplementation of Che-SeNPs significantly (p < 0.0001) increased body weight, body weight gain, and feed conversion ratio, but decreased feed intake (p < 0.0001) compared to the control group. The highest values of growth performance were recorded in the group fed 0.4 g Che-SeNPs g/kg feed. Che-SeNPs levels did not affect the carcass traits, relative organs (except liver), or blood hematology (except platelet count and hemoglobin level) of quails. Plasma total protein, albumin, aspartate amino transferase (AST), and urea values were not affected by dietary Che-SeNPs, but alanine aminotransferase and lactate dehydrogenase values declined. Globulin and creatinine values were linearly increased with the inclusion of Che-SeNPs (0.4 and 0.6 g/kg) in quail diets compared to the control. The supplementation of Che-SeNPs in quail diets significantly improved (p < 0.05) the plasma lipid profile and activities of antioxidant enzymes compared to the control group. Immunoglobulin G values of Che-SeNPs (0.4 and 0.6 g/kg) were higher (p < 0.05) than those in the control group. The groups fed diets supplemented with Che-SeNPs showed lower (p < 0.0001) total bacterial count, total yeast and molds count, Coliform, Escherichia coli, Enterococcus spp., and Salmonella spp. colonization, and higher (p = 0.0003 and 0.0048) lactic acid bacteria counts than those in the control group. In conclusion, Che-SeNPs supplemented up to 0.4 g/kg can improve the performance, lipid profile, antioxidant indices, and immunity, as well as decrease intestinal pathogens in quails during the fattening period (1–5 weeks of age).

scholarly journals Impact of Siberian larch dihydroquercetin or dry distilled rose petals as feed supplements on 8 lamb's growth performance, carcass characteristics and blood count parameters.

2020 ◽  
Author(s):  
N. Zh. Stancheva ◽  
J. L. Nakev ◽  
D. B. Vlahova-Vangelova ◽  
D. K. Balev ◽  
S. G. Dragoev
Keyword(s):  
Growth Performance ◽  
Blood Count ◽  
Siberian Larch ◽  
Live Weight ◽  
Blood Parameters ◽  
Control Group ◽  
Rose Petals ◽  
The Impact ◽  
Group D ◽  
Experimental Group

Abstract The objective of this study was to determine the impact of dihydroquercetin from Siberian larch and dry distilled rose petals (DDRP) on growth performance, carcasses characteristics and blood parameters of lambs from the Bulgarian Dairy Synthetic population sheep. For the purpose of the study there were used 30 clinically healthy male lambs aged 65 days, levelled by live weight. They were housed in a totally indoor barn and were divided into one control and two experimental groups, each consisting of 10 animals that were fed for 50 days. The control group (C) was fed ground alfalfa + granulated compound feed. The experimental groups (D) and (R) were fed on the same diet supplemented either with 7.5 mg dihydroquercetin/kg/day or with 545 mg DDRP/kg/day respectively. The carcass yield of lambs having consumed dihydroquercetin or DDRP compared to lambs from control group C do not have significant differences (p > 0.05). The carcass conformation of C or R groups lambs do not differ (70% - class P, 30% - class O). The 90% of lamb's carcasses from the experimental group D were classified in class P. The dihydroquercetin feeding increases the relative fat content (p ≤ 0.01) of lamb carcasses but adversely affects their conformation. No significant differences (p > 0.05) were found between 1st h and 24th h post-mortem pH of control group C and experimental group D. Compared to them the pH values of the experimental group R were by 0.14-0.15 pH units lower (p ≤ 0.05). No significant differences (p > 0.05) were found in the blood count of the three studied groups of lambs. Exceptions were made for haemoglobin (HGL) in the experimental group D which were with 6-7 g/l higher (p ≤ 0.05) than these in control group C and experimental group R and the blood glucose (GLU) in the experimental group R which is with 0.25-0.28 mmol/l lower than determined in control group C and experimental group D.

scholarly journals Use of Chemical Nano Selenium as Antibacterial and Antifungal Agent in Quail Diets and its Effect on Growth, Carcasses, Antioxidant, Immunity and Caecal Microbe

2021 ◽  
Author(s):  
Mahmoud Alagawany ◽  
M. T. El-Saadony ◽  
S. S. Elnesr ◽  
M. Farahat ◽  
G. Attia ◽  
...  
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Bacterial Count ◽  
Intestinal Pathogens ◽  
The Impact

Abstract Nano-minerals are used to enhance mineral bioavailability, which helps improve animal growth and health. The use of chemical nano-selenium (Che-SeNP) has lately attracted great scientific interest, mainly due to its potential benefits for poultry. The current study was conducted to investigate the impact of the dietary supplementation of Che-SeNPs on growth performance, carcass traits, blood constituents, antioxidant status, immunity, and gut microbiota of quails. A total of one week-old 180 Japanese quails were randomlydistributed into four equal groups, and each group consisted of 45 unsexed birds with five replications (nine birds each). The first group was fed a basal diet without supplementation (0 g/kg Che-SeNP), and the second, third, and fourth groups were fed diets containing 0.2, 0.4, and 0.6 g/kg Che-SeNP, respectively. The results showed that the dietary supplementation of Che-SeNP significantly (P < 0.05) increased body weight, body weight gain, and feed conversion ratio but decreased feed intake (P < 0.001) compared to the control group. The highest values of growth performance were recorded in the group fed 0.4 g Che-SeNP g/kg feed. Che-SeNP levels did not affect carcass traits, relative organs (except liver), or blood hematology (except platelet count and hemoglobin level) of quails. Plasma total protein, albumin, aspartate aminotransferase (AST) and urea values were not affected by dietary Che-SeNP, but alanine aminotransferase and lactate dehydrogenase values declined. Globulin and creatinine values were linearly increased with the inclusion of Che-SeNP (0.4 and 0.6 g/kg) in quail diets compared to the control. The supplementation of Che-SeNP in quail diets significantly improved (P < 0.05) the plasma lipid profile and activities of antioxidant enzymes compared to the control group. Immunoglobulin G values of Che-SeNPs (0.4 and 0.6 g/kg) were higher (P < 0.05) than those in the control group. The groups fed diets supplemented with Che-SeNP showed lower (P < 0.0001) total bacterial count, total yeast and molds count, Coliform, Escherichia coli, Enterobacter, and Salmonella colonization, and higher (P = 0.0003 and 0.0048) lactic acid bacteria counts than those in the control group. In conclusion, dietary supplementation with Che-SeNP can improve the performance, lipid profile, antioxidant indices, and immunity, as well as decrease intestinal pathogens in quails.

scholarly journals Effect of new livestock feeds' phytonutrients on productivity, carcass composition and meat quality in pigs.

2019 ◽  
Author(s):  
S.G. Ivanova ◽  
J.L. Nakev ◽  
T.I. Nikolova ◽  
D.B. Vlahova-Vangelova ◽  
D.K. Balev ◽  
...  
Keyword(s):  
Growth Performance ◽  
Meat Quality ◽  
Nutrient Digestibility ◽  
Carcass Composition ◽  
Feed Consumption ◽  
Control Group ◽  
Histological Structure ◽  
Rosa Damascena ◽  
Rose Petals ◽  
The Impact

Abstract Improving pig growth performance, nutrient digestibility and pork quality is a continual goal of the pig breeding. For this reason, an increasing interest in using phytonutrients has been observed recently. The objective of this study was to determine the impact of Larix sibirica dihydroquercetin or dry distilled Rosa damascena petals on growth performance, carcass composition, meat quality, blood characteristics and histological changes in ovaries and liver in native Danube White fattening pigs. A total of 120 pigs were used in the experiment with duration of 45 days prior to harvest. The pigs were divided into five groups. The control group (C) was fed basal diet. The other four experimental groups were fed with the same diets containing either 3.5 or 7.5 mg dihydroquercetin/kg/d and 0.255 or 0.545 g dry distilled rose petals/kg/d. The supplementations with dihydroquercetin or dry distilled rose petals increased average body weight by 7.74-9.05%, average daily gain by 27.06-30.13%, and feed to gain ratio by 12.53-15.99% and decreased feed consumption by 5.24-13.84% and average liver weight by 10.53-21.12% compared to the control group. Two supplementations didn't cause pathological changes in histological structure of pigs' liver and ovaries, and didn't influence pH values and proximate composition of m. Longissimus thoracis and m. Semimebranosus. No pH determined stress-induced muscle damage was found and the pork carcasses were classified in classes E and U. The two used supplements reduced the blood LDL cholesterol by 13.27-14.29% as well as increased erythrocytes, platelets, haematocrit, mean red blood cell count, mean haemoglobin concentration in erythrocytes, triglycerides and total cholesterol.

scholarly journals Effect of the Aerobic Denitrifying Bacterium Pseudomonas furukawaii ZS1 on Microbiota Compositions in Grass Carp Culture Water

Water ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1329
Author(s):  
Wangbao Gong ◽  
Shuwei Gao ◽  
Yun Zhu ◽  
Guangjun Wang ◽  
Kai Zhang ◽  
...  
Keyword(s):  
Treatment Group ◽  
Grass Carp ◽  
High Throughput Sequencing ◽  
Ammonia Nitrogen ◽  
Control Group ◽  
Control Groups ◽  
Aquaculture Water ◽  
And Control ◽  
The Impact ◽  
Microbiota Structure

Background: Although functional bacteria are widely used in aquaculture water treatment, whether they affect the indigenous microbiota and whether the impact is persistent remain unclear. Therefore, we aimed to explore the denitrification effect of Pseudomonas furukawaii ZS1 isolated from a grass carp culture pond in nitrogen-rich aquaculture water, and determine whether its effect on the microbiota structure of the aquaculture water was persistent. Methods: Three each of treatment and control groups were set up, and P. furukawaii ZS1 was added to the treatment group. The concentrations of ammonia nitrogen, nitrite, and nitrate, and the pH of each sample were measured for eight consecutive days. Changes of microbiota composition in the water were analysed via high-throughput sequencing. Results: Ammonia, nitrite, and nitrate concentrations were substantially lower in the treatment group than in the control group. There were significant differences in the microbiota structure between treatment and control groups, especially on days 2–7 after adding P. furukawaii ZS1. Furthermore, significantly enriched bacterial genera in the treatment group were initially higher in number than inhibited genera, but subsequently reverted to being lower in number. Conclusions: These results provide theoretical guidance for the effective use of P. furukawaii ZS1 to control aquaculture water.

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