Labia and Lingua Histopathology of Bali Cow (Bos sondaicus) on Hepatogenous Photosensitization Symptoms in Pakutatan Village, Jembrana, Bali

2018 ◽  
Vol 2 (2) ◽  
pp. 31
Author(s):  
Kadek Mardika ◽  
Iriani Setyawati ◽  
Dwi Ariani Yulihastuti
Keyword(s):  
Histopathological Examination ◽  
Cell Damage ◽  
Lantana Camara ◽  
Bacterial Invasion ◽  
Average Percentage ◽  
Large Numbers ◽  
Hematoxylin And Eosin ◽  
Lantadene A ◽  
One Way Anova ◽  
Paraffin Method

Hepatogenous photosensitization is one of the ruminant diseases with symptoms of dermatitis or eczema of the skin accompanied by liver damage.  The disease is caused by the compounds of toxic lantadene A and lantadene B which are secondary metabolites of Lantana camara plant.  This research was carried out on January 2017. The material used in this study was preserved organs of the labia and lingua of dead three year old cow (Bos sondaicus).  Samples were taken from Pakutatan Village, Jembrana, Bali.  Identification of organ samples, histological preparation and histopathological examination were conducted at the Disease Investigation Center (DIC) 6, Denpasar, Bali.  The preparation of labia and lingua samples used the paraffin method with Hematoxylin and Eosin staining.  The tissue structural damages found were necrosis, inflammatory cell infiltration, congestion and tissue bleeding.  The data obtained were analyzed statistically by One Way Anova test with a confidence level of 95%.  Based on the results,  dead Bali cow which consumed a large numbers of Lantana camara plants showed that the highest number of cell damage was at the picnotic stage of cell necrosis (cell death) in the labia organ significantly (P<0.005), while the highest bacterial invasion was found in the labia organ with an average percentage of 12.40%.

Observations on a field outbreak of Pox Virus Infection in young Nile crocodiles (Crocodylus Niloticus)

1991 ◽  
Vol 62 (1) ◽  
pp. 26-29 ◽  
Author(s):  
F. W. Huchzermeyer ◽  
K. D.A. Huchzermeyer ◽  
J. F. Putterill
Keyword(s):  
Virus Infection ◽  
Histopathological Examination ◽  
Skin Lesions ◽  
High Morbidity ◽  
Microscopical Examination ◽  
Virus Particles ◽  
Large Numbers ◽  
Intracytoplasmic Inclusions ◽  
Electron Microscopical ◽  
Scanning Electron

A field outbreak of pox virus infection in juvenile Nile crocodiles (Crocodylus niloticus), in which high morbidity and negligible mortality occurred, is described. Histopathological examination of the skin lesions revealed numerous large intracytoplasmic inclusions in the dermis and a very mild dermal inflammatory reaction. Scanning electron microscopical examination of the skin revealed the presence of large numbers of virus particles in the inclusions. Skin lesions persisted for 5 to 6 months.

scholarly journals Lung Histopathology of Laying Hens Infected by Colibacillosis in the Animal Cages Experiments of the Disease Investigation Center 6, Denpasar, Bali

2019 ◽  
Vol 3 (2) ◽  
pp. 25
Author(s):  
Kadek Ayu Trisna Yanti ◽  
Iriani Setyawati ◽  
Ni Putu Adriani Astiti
Keyword(s):  
Laying Hens ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Gallus Gallus Domesticus ◽  
Directorate General ◽  
Lung Histopathology ◽  
Live Stock ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Neutral Buffer Formalin

This study aimed to determine the lungs histopathology of laying hens (Gallus gallus domesticus) at the Animal Cage Experiments in the Disease Investigation Center 6, Directorate General of Live Stock (DIC-6 DGLS), Denpasar, Bali, which died from colibacillosis infection. Sample of lungs were cut transversely then put into 10% of Neutral Buffer Formalin, then processed histologically by paraffin method and stained with Hematoxylin-Eosin. Observation under microscope (magnification 100x and 400x) was done for histopathological examination. Laying hens died from colibacillosis infection showed that their lungs were infected by colibacillosis, and there were found 62.50% of necrosis, 75% of inflammatory cells infiltration and 80% of hemorrhage in the lungs.

scholarly journals Histopathological study of endometrium in abnormal uterine bleeding

2012 ◽  
Vol 2 (4) ◽  
pp. 297-300 ◽  
Author(s):  
S Bhatta ◽  
AK Sinha
Keyword(s):  
Histopathological Examination ◽  
Abnormal Uterine Bleeding ◽  
Uterine Bleeding ◽  
Common Finding ◽  
Histopathological Study ◽  
Endometrial Sampling ◽  
Proliferative Endometrium ◽  
Malignant Lesions ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Background: Histopathological evaluation of endometrial samples is essential in the workup of abnormal uterine bleeding. This study was carried out to evaluate patterns of endometrial histological findings in women with abnormal uterine bleeding and to correlate histopathological findings with clinical features.Materials and Methods: One hundred and twenty two patients with diagnosis of abnormal uterine bleeding underwent endometrial sampling. The slides stained with Hematoxylin and Eosin stain were studied.Results: The predominant endometrial histopathological finding was proliferative endometrium 32 cases (26.23%) followed by simple hyperplasia without atypia 22 cases (18.03%). Malignant lesions were more common in patients more than 40 years of age and comprised of 7 cases (5.74%) of all cases. Atrophic endometrium was most common finding in postmenopausal bleeding 8 cases (28.6%) followed by endometrial carcinoma 5 cases (17.9%).Conclusion: Histopathological examination of endometrium should be done generously in women presenting with abnormal uterine bleeding especially after the age of 40 years to rule out malignant pathology.Journal of Pathology of Nepal (2012) Vol. 2, 297-300DOI: http://dx.doi.org/10.3126/jpn.v2i4.6882

Synergistic Effects of Sequential Carbon Dioxide and Neodymium: Yttrium Aluminum Garnet Laser Injuries

1987 ◽  
Vol 96 (1) ◽  
pp. 47-52 ◽  
Author(s):  
William J. Primrose ◽  
Graeme A. McDonald ◽  
Charles W. Vaughan ◽  
Michael J. O'Brien ◽  
M. Stuart Strong
Keyword(s):  
Carbon Dioxide ◽  
Nd:Yag Laser ◽  
Yttrium Aluminum Garnet ◽  
Tissue Injury ◽  
Cell Damage ◽  
Synergistic Effects ◽  
Biological Effects ◽  
Rhodamine 123 ◽  
Initial Application ◽  
Hematoxylin And Eosin

The carbon dioxide and neodymium:yttrium aluminum garnet lasers have well documented but characteristically different biological effects, yet little is known about their cumulative, synergistic, or paradoxical effects when used sequentially on living tissue. Using a Merrimack ML 880 laser, a series of superimposed CO2 and Nd:YAG lesions in various combinations were produced on the undersurface of dog tongues. Therapeutic time and power settings were chosen and the number of applications varied, with suitable controls. Observations and measurements were made on acute, healing, and healed lesions. All lesions were excised and submitted for routine hematoxylin and eosin histology. Acute lesions were also assessed for cell viability using rhodamine 123 as a supravital marker. The results show that, even though all the lesions eventually heal, the actual cell damage produced by the Nd:YAG laser is much more than is suggested by the size of the acute lesion. This cell damage can be reduced by the surface carbonization produced by initial application of the CO2 laser. Higher surface temperatures are reached in this combination with less fibrosis and scarring than equal energy counterparts where the Nd:YAG laser was applied first. The knowledge of these synergistic effects can be used to advantage in the clinical setting. The rhodamine 123 technique also appears to be a valid measure of acute thermal tissue injury.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals Histological and Epidemiological study on Mycobacterium tuberculosis in Nineveh Governorate

2019 ◽  
Vol 24 (1) ◽  
pp. 13
Author(s):  
Kawkab Adris Mahmood
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Epidemiological Study ◽  
Sex Education ◽  
Histopathological Examination ◽  
Age Groups ◽  
Tissue Sections ◽  
Hematoxylin And Eosin ◽  
Main Factors ◽  
Almost All ◽  
The Relationship

Tuberculosis (TB) is a common infection especially in developing countries and can involve almost all bodily systems. Iraq has been identified as middle TB burden country in the world. The aims of the study are to determine the main factors that lead to prevalence of TB in people living in Nineveh governorate, to study the histopathological effect of M. tuberculosis on the tissues. The objective of epidemiological study is  to find the relationship between M. tuberculosis and age, sex, education level, occupation, type of TB and sectors of people living in Nineveh governorate. This study included 100 patients infected with Mycobacterium tuberculosis ( 51 males and 49 females ) the age of the patients ranged from 1–75 years through the period from september 2010 to september 2011. In order to determine the main factors that lead to TB infection, data collected from all 100 patients infected with TB. Tissue sections were taken from patients with active TB from (lung, lymph node and breast mass) and fixed in (10%) formaldehyde and (4µm) paraffin blocks sections stained with hematoxylin and eosin or Ziehl Neelsen stain for histopathological examination. Data for epidemiological study of patients diagnosed with TB were  collected from consultant clinic for chest and respiratory diseases in Nineveh governorate over a 16-year period (1994-2010). The results showed that The main factors that lead to TB infection were diabetes with a percentage of (25%) of the patients, followed by smoking with a percentage of (20%) . Changes in the tissues infected with tuberculosis is the formation of tuberculous granulomas .The result of epidemiological study showed that the number of Tuberculosis cases in Nineveh governorate increased between 1994-2002 and decreased between 2002-2010. The number of TB cases in males is more than in females . The main age groups infected with TB was the productive ages (15-34 years). Pulmonary TB is more than Extrapulmonary TB .The highest percentage of TB in sectors of Nineveh governorate (25%) for each of left sector and right sector, followed by (13.7%) in Sinjar.   http://dx.doi.org/10.25130/tjps.24.2019.003    

scholarly journals Amelioration of Cell Phone and Wi Fi induced Pancreatic Damage and Hyperglycemia (Diabetes Mellitus) with Pomegranate and Vit E in Rats

2021 ◽  
pp. 204-215
Author(s):  
Hadiya Sibghatullah ◽  
Sibghatullah Muhammad Ali Sangi ◽  
Elsamoual Ibrahim Ahmedani ◽  
Ali Alqahtani ◽  
Abdulhakim Bawadekji ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Ionizing Radiation ◽  
Cell Phone ◽  
Histopathological Examination ◽  
Cell Damage ◽  
Cell Phones ◽  
Albino Rats ◽  
Pancreatic Cell ◽  
Rat Pancreas ◽  
Wistar Albino Rats

Background: Type 2 Diabetes Mellitus has become a global concern. To date numerous studies have been conducted but little literature is available to explain the effects of mobile phone radiation on pancreas, where from Insulin is secreted. In Some studies, effects of ionizing radiation have been examined and established the relationship between cell phone exposure and cell damage. Objectives: Objectives of study were to observe the effects of mobile phones, connected with WiFi on the pancreas. Methods: 40 male Wistar Albino rats were exposed tomobile phones connected with Wi-Fi for eight weeks. Results: The histopathological examination of the rat pancreas revealed that, exposure of rats to cell phones and Wi-Fi causes significant damage to the rat pancreas. Conclusion: The ionizing radiation emitted from cell phones and WiFi causes increase in oxidative stress leading to inflammation and pancreatic cell death that may affect glucose homeostasis.

scholarly journals Prevalence of bovine sarcocystosis in slaughtered cattle in Duhok abattoir, Iraq

2020 ◽  
Author(s):  
Mohammed S. Shukur ◽  
Shivan N. Hussein ◽  
Teroj A. Muhamed
Keyword(s):  
Life Cycle ◽  
Prevalence Rate ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Sarcocystis Species ◽  
Large Numbers ◽  
Muscle Tissues ◽  
Significant Difference ◽  
Peptic Digestion ◽  
Infected Muscle

Background: Sarcocystosis or infection with Sarcocystis species is a parasitic zoonotic disease caused by cyst forming coccidian intracellular protozoa that caused by different species of Sarcocystis. The cyst forming parasite has obligatory two hosts life cycle including carnivorous as definitive host and omnivorous or herbivorous as intermediate host. The parasitic infestation causes serious health problems and economical loses because of abortion in pregnant animals, carcass condemnation after slaughtering due to severe emaciation and pathological lesions, low quality of meat, milk and wool, as well as restriction on animal importation by authorities.Methods: The present work was conducted to study the prevalence of sarcocysts infection in slaughtered cattle at Duhok abattoir, Iraq. Muscle samples from different organs comprising esophagus, diaphragm and heart were collected from 150 cattle aged from one to two years old. Different techniques were used for detection of macroscopic and microscopic types of sarcocysts. The techniques including inspection by naked eye, peptic digestion method, muscle mincing and squash preparation and staining with giemsa stain, as well as histopathological examination.Results: The overall prevalence of infected muscle samples was 76%. The infection rate of microscopic type of sarcocyst was 41.3% in heart, 92% in diaphragm and 94% in oesophagus. The histopathological examination of infected muscle tissues revealed mild infiltration of inflammatory cells and slight degeneration of muscle fibers. Significant difference (p≤0.05) was recorded between the prevalence rate of macrocysts and microcysts of sarcocyst but there was no significant difference (p≥0.05) in the prevalence rate of sarcocystis infection among different organs.Conclusions: The results of the current study indicated a high prevalence of sarcocystis infection among slaughtered cattle in Duhok province, Iraq, that could be due existence of large numbers of dogs and cats around the slaughter house which are involved in life cycle of the parasite and spread of the infection.

Phytoconstituents of Lantana camara L.: Rekindling Hope in the Cancer Treatment

2021 ◽  
Vol 18 ◽  
Author(s):  
Ishita Kathuria ◽  
Mit Joshi ◽  
Bhoomika M. Patel ◽  
Mahaveer Dhobi
Keyword(s):  
Lantana Camara ◽  
In Vivo Studies ◽  
The North ◽  
Proliferative Effect ◽  
Tropical America ◽  
Oleanonic Acid ◽  
Southern Georgia ◽  
Lantadene A

Background: Lantana camara L. belongs to the family Verbenaceae. It originated in Tropical America in Southern Georgia and to the North of Texas and was introduced in Calcutta, India in the year 1809 as an ornamental hedge. The plant L. Camara is also distributed in Southeast Asia, China, Australia, Brazil, West Indies, Kenya, Mexico, East Africa, Tanzania. Many of its phytoconstituents possess medicinal properties which are used traditionally to treat fever, uterine hemorrhage, and excess menstrual discharge, chronic ulcers, rheumatism, gonorrhea, toothache, gastrointestinal pain, etc, and has been used in Brazil for curing malaria, mange, headaches, colds, and fevers. Objectives: The review elaborates traditional practices, phytochemistry of Lantana camara L. along with the role of Lantana camara in various types of cancers. Method: The data on L. camara was collected through different online databases like Web of Science, PubMed, Science Direct, Springer, and Google Scholar. Results: Major phytoconstituents isolated from the plant shows anticancer activity specially lantadene A-D, icterogenin, oleanolic acid, lantacamaric acid A, B, oleanonic acid, etc. In vitro and in vivo studies demonstrate its potential for various cancers. Certain extracts, isolated compounds, and their semi-synthetic derivatives have depicted a significant cytotoxic and anti-proliferative effect. Conclusion: Clinical studies are not yet established, therefore, making it crucial to direct future researches in that area.

ChemInform Abstract: Structure of Lantadene A, the Major Triterpenoid of Lantana Camara, Red Variety.

ChemInform ◽  
2010 ◽  
Vol 22 (29) ◽  
pp. no-no
Author(s):  
V. PATTABHI ◽  
N. SUKUMAR ◽  
O. P. SHARMA
Keyword(s):  
Lantana Camara ◽  
Abstract Structure ◽  
Red Variety ◽  
Lantadene A
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