scholarly journals Activity of Javanese Ginger, Turmeric, Garlic, and Pomegranate Flower on LDL-C and Total-C on Dyslipidemia Model Rats

2021 ◽  
Vol 9 (2) ◽  
Author(s):  
Larissa Larissa ◽  
Penny Setyawati Martioso ◽  
Diana Krisanti Jasaputra
Keyword(s):  
Vitamin D3 ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
Ethanol Extract ◽  
Laboratory Research ◽  
Control Group ◽  
Experimental Animals ◽  
Different Types ◽  
Male Wistar Rats ◽  
Experimental Laboratory

High levels of LDL cholesterol are risk factors for coronary heart disease. Different types of medicinal plants have hypolipidemic effects. The study aimed to compare the potential of Javanese ginger ethanol extract, turmeric, garlic, and pomegranate flowers with rosuvastatin on levels of LDL cholesterol (LDL-C) and total cholesterol (total-C) male Wistar rats dyslipidemia models. This experimental laboratory research was conducted in Maranatha Animal Research Laboratory Bandung and was carried out in January–December 2020. The experimental animals were divided into six groups (n=5): the control group, the Javanese ginger group, the turmeric group, the garlic group, the pomegranate flower group, and the comparison control group. The induction given to experimental animals was administering vitamin D3, a high-fat diet, and propylthiouracil for 14 days. The results showed that the administration of 175 mg/kg BW of garlic ethanol extract (−44.85%), pomegranate flowers (−58.74%), and rosuvastatin (−40.00%) reduced LDL-C compared to control (p<0.05). The administration of 175 mg/kg BW of Javanese ginger ethanol extract (−15.16%), turmeric (−14.02%), garlic (−22.80%), pomegranate flower (−65.24%), and rosuvastatin (−18.70%) reduced total-C compared to controls (p<0.05). The conclusion is that garlic and pomegranate flowers lowered LDL-C, while Javanese ginger, turmeric, garlic, and pomegranate flowers reduced total-C. AKTIVITAS TEMULAWAK, KUNYIT, BAWANG PUTIH, DAN BUNGA DELIMA TERHADAP K-LDL DAN K-TOTAL PADA TIKUS MODEL DISLIPIDEMIAKadar kolesterol LDL yang tinggi adalah faktor risiko penyakit jantung koroner. Berbagai jenis tanaman obat memiliki efek hipolipidemik. Penelitian ini bertujuan membandingkan potensi ekstrak etanol temulawak, kunyit, bawang putih, dan bunga delima dengan rosuvastatin pada kadar kolesterol LDL (K-LDL) dan kolesterol total (K-total) tikus Wistar jantan model dislipidemia. Penelitian laboratorium eksperimental ini dilakukan di Laboratorium Penelitian Hewan Maranatha Bandung dan dilakukan pada Januari–Desember 2020. Hewan coba dibagi menjadi enam kelompok (n=5), yaitu kelompok kontrol, kelompok temulawak, kelompok kunyit, kelompok bawang putih, kelompok bunga delima, dan kelompok  pembanding. Induksi yang diberikan kepada hewan coba adalah pemberian vitamin D3, pakan lemak tinggi, dan propyltiouracil selama 14 hari. Hasil penelitian menunjukkan bahwa pemberian 175 mg/kgBB ekstrak etanol bawang putih (−44,85%), bunga delima (−58,74%), dan rosuvastatin (−40,00%) mengurangi K-LDL dibanding dengan kontrol (p<0,05). Pemberian 175 mg/kgBB ekstrak etanol temulawak (−15,16%), kunyit (−14,02%), bawang putih (−22,80%), bunga delima (−65,24%), dan rosuvastatin (−18.70%) mengurangi K-total dibanding dengan kontrol (p<0,05). Kesimpulannya, bunga bawang putih dan delima menurunkan K-LDL, sedangkan temulawak, kunyit, bawang putih, dan bunga delima menurunkan K-total.

scholarly journals Test of the Effectiveness of Anti-Dyslipedemia Extract Ethanol Mangosteen Peel (Garcinia Mangostana L.) in Male Mice Fed a High-Fat Diet

2021 ◽  
Vol 4 (1) ◽  
pp. 1-12
Author(s):  
Zhang Yu ◽  
Florenly ◽  
Liena ◽  
Fioni
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Ethanol Extract ◽  
Control Group ◽  
High Fat ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Entire Treatment

Dyslipidemia is a major risk factor for atheroscler heart disease, stroke, and is often defined as abnormalities or disruption of lipid metabolism. Garcinia mangostana L. is famous for its anti-inflammatory properties and is used in the treatment of skin infections and wounds. The main phytochemicals present in this species are anisoprenylated xanthone, many reports of biological effects, such as antioxidant, pro-apoptosis, anti-proliferative, anti-nosiseptif, anti-inflammatory, neuroprotective, hypoglycemic, and anti-obesity. This study aims to find out the effectiveness of mangosteen peel ethanol extract as an anti-dyslipidemia in male wistar rats given a high-fat diet. This type of research is experimental with a Pre-test approach and Post-test group only control design. The samples used were mangosteen peel ethanol extract and male wistar rats, with the size of the sample calculated with Federer's formula. Analyze data with the One-Way Anova Test if the data is normally distributed with advanced tests in the form of Post Hoc Tukey HSD tests to see real differences between treatments. The results of the total cholesterol study in the entire treatment group of mice showed a significant difference in P values < 0.05. Triglyceride levels in the entire treatment group also showed significant differences, this can be seen from the value of P < 0.05 (Value P = 0.029). LDL levels also showed significant differences across the treatment group, which can be seen from the P value of < 0.05. HDL levels make a significant difference in the value of P < 0.05 (Value P = 0.029). SGOT and SGPT levels in the entire group of rat treatment showed significant differences, this was seen from the value of P < 0.05. The conclusion that mangosteen peel ethanol extract significantly lowered total cholesterol, triglyceride levels, LDL levels, SGOT levels compared to the control group. Mangosteen peel ethanol extract can significantly increase HDL levels compared to the control group.

Hypolipidemic effects of Hypericum Scabrum extract on the serum lipid profile and obesity in high-fat diet fed rats

2020 ◽  
pp. 1-7
Author(s):  
Nafiseh Faraji ◽  
Ahmad Ganji ◽  
Neda Heshami ◽  
Iraj Salehi ◽  
Ahmad Haddadian ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Biochemical Analysis ◽  
Ldl Cholesterol ◽  
Hdl Cholesterol ◽  
Control Group ◽  
High Fat ◽  
Male Wistar Rats ◽  
Hypericum Scabrum ◽  
Elevated Lipid ◽  
And Oxidative Stress

Diets included high-fat (HFD) and high calories intake is correlated with greater risk of obesity and oxidative stress, which lead to increase the risk of related diseases such as cardiovascular and metabolic disease. In the present study, we have examined the hypolipidemic activity of Hypericum Scabrum extract on HFD fed rats. Fifty-four male Wistar rats divided into six groups: 1) control, 2) H. Scabrum extract (100 mg/kg gavage per day), 3) H. Scabrum extract (300 mg/kg), 4) HFD, 5) HFD and H. Scabrum extract (100 mg/kg), 6) HFD and H. Scabrum extract (300 mg/kg). The groups were fed their diet and treatment for 3 months. Biochemical analysis showed elevated lipid serum profile in HFD rats compared to control group. H. Scabrum extract supplementation significantly ameliorated triglyceride, total cholesterol and LDL-cholesterol. H. Scabrum extract supplementation leading to increase HDL-cholesterol in HFD treated groups. This experiment showed that H. Scabrum extract decreased HFD complications and might be beneficial herbal drug for treatment of hyperlipidemia and obesity.

Pengaruh Pemberian Ekstrak Anggur Laut terhadap Penurunan Kadar Kolesterol LDL Rattus norvegicus Jantan yang Mendapat Diet Tinggi Lemak

2020 ◽  
Vol 17 (2) ◽  
pp. 192
Author(s):  
RONALDO LAU ◽  
SULISTIANA PRABOWO ◽  
RIAMI RIAMI
Keyword(s):  
Cholesterol Level ◽  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
White Male ◽  
Standard Diet ◽  
High Fat ◽  
Caulerpa Racemosa ◽  
Significant Difference ◽  
Male Wistar Rats

<p align="justify"><strong>ABSTRACT</strong><strong></strong></p><p align="justify"><strong>Background</strong>: High fat diet increase the absorption of lipid in the intestinum, that can lead to increase LDL cholesterol level in the blood. Sea grapes extract (<em>Caulerpa racemosa</em>) contains antioxidant polyphenolic group that can reduce MTP and ACAT-2 in the body that can decrease LDL cholesterol level in the blood.The purpose of this study is to know the effect of sea grapes extract  on decreasing LDL cholesterol of white male Wistar rats (<em>Rattus norvegicus</em>) fed with high fat diet.</p><p align="justify"><strong>Method</strong>:  24 white male Wistar rats, that divided into 3 groups: 1) group of rats fed with standard diet for 28 days; 2) group of rats fed with high fat diet for 28 days; 3) group of rats fed with high fat diet for 28 days and given 10 gram/kg body weight/day of sea grapes extract on 15<sup>th</sup>-28<sup>th</sup> days. Then the blood LDL cholesterol level measured on the 29<sup>th</sup> day.</p><p align="justify"><strong>Result :</strong> One-Way ANOVA Test showed there was significant difference (p=0.004) of LDL level between the group of rats fed with standard diet (12.37 mg/dl) compared to group of rats fed with high fat diet (17.87 mg/dl). There was significant difference (p=0.001) of LDL level between the group of rats fed with high fat diet (17.87 mg/dl) compared to group of rats fed with high fat diet and sea grapes extract (10.12 mg/dl).</p><p align="justify"><strong>Conclusion: </strong>high fat diet significantly increase blood LDL cholesterol level and sea grapes extract (<em>Caulerpa racemosa</em>) significantly decrease blood LDL cholesterol level.</p><p align="justify"> </p><p align="justify"><strong>Keywords :</strong>Sea grapes extract, LDL cholesterol, high fat diet</p>

Expression and Significance of Lipin1 and AMPKα in Hepatic Insulin Resistance in Diet-Induced Insulin Resistance Rats

2012 ◽  
Vol 120 (02) ◽  
pp. 84-88 ◽  
Author(s):  
S. Chen ◽  
X. Zhuang ◽  
Y. Liu ◽  
A. Sun ◽  
C. Chen
Keyword(s):  
Insulin Resistance ◽  
High Fat Diet ◽  
Diet Group ◽  
Hepatic Insulin Resistance ◽  
Control Group ◽  
Mrna Levels ◽  
Ampk Signaling ◽  
Protein Levels ◽  
Obese Rats ◽  
Male Wistar Rats

AbstractLipin1, a lately indentified adipokine, may link obesity with insulin resistance and diabetes. The present study aimed to investigate the changes and significance of lipin1 expression and lipin1-AMPK signaling in diet-induced hepatic insulin resistance.24 4-week-old Male Wistar rats were randomly divided into 2 groups: (1) control group (CO), (2) high-fat diet group (HF). Insulin sensitivity was evaluated by hyperinsulinemic-euglycemic clamp technique. The mRNA levels of α1 and α2 subunit of AMPKα as well as Lipin1 were measured using Real-time RT-PCR. The activities of AMPKα and Akt were evaluated by detection of p-AMPKα (Thr-172) and p-Akt (ser473) by Western blot.After treatment of 4 months, HF group showed significantly increased levels of body weight, fasting plasma glucose and insulin levels; Plasma and liver total cholesterol (TC), triglycerides (TG) levels were also markedly elevated; Lipin1 expression at both mRNA and protein levels were significantly deceased. Compared with CO group, the mRNA and protein levels of AMPKα1 and AMPKα2 were not changed, whereas the p-AMPK (Thr-172) and p-AKT (ser473) levels in liver were significantly decreased in HF group.These findings indicated that the decrease in lipin1 expression and AMPKα activation may contribute to hepatic insulin resistance in diet-induced obese rats.

scholarly journals The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol

2017 ◽  
Vol 15 (2) ◽  
pp. 196
Author(s):  
Much Ilham Novalisa Aji Wibowo ◽  
Nur Aeni ◽  
Zidna Mazayatul Huda ◽  
Nunuk Aries Nurulita
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Group Iv ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Male Wistar Rats ◽  
Hepatoprotective Agent ◽  
Better Than

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.

scholarly journals Mechanism of Pancreatic Islet Number Improvement on Hyperglycemic Wistar Rats (Rattus norvegicus) After Golden Sea Cucumber (Stichopus hermanii) Extract Administration

2020 ◽  
Vol 9 (3S) ◽  
pp. 320-324
Keyword(s):  
Blood Sugar ◽  
Pancreatic Islet ◽  
Blood Sugar Level ◽  
Sea Cucumber ◽  
Histopathological Examination ◽  
Ethanol Extract ◽  
Male Rats ◽  
Laboratory Research ◽  
Control Group ◽  
Sugar Levels

Diabetes mellitus constitutes a metabolic disorder indicated by a decrease in insulin secretion, insulin insensitivity, or a combination of both. Golden sea cucumber (Stichopus hermanii) is a natural resource of Indonesian waters with benefit for reducing blood sugar levels. This research aimed to determine the anti-hyperglycemic mechanism of golden sea cucumber.This study used a experimental laboratory research with Post-test Only Control Group. The sample of this study consisted of 25 male rats aged 60-70 days, divided into 5 groups: K(-), K(+), P1, P2, and P3 randomly. Rats from K(+), P1, P2, and P3 groups were injected with streptozotocin (STZ) with a dose of 50 mg/kg BW. Treatment was administered for 21 days. K(-) and K(+) groups were fed normally while P1, P2 and P3 groups were given golden sea cucumber 80% ethanol extract with a dose of 4,25 mg/kgBW/day, 8,50 mg/kgBW/day and 12,75 mg/kgBW/day respectively. On the 22nd day, fasting blood sugar level was measured and the number of pancreatic islet was counted with histopathological examination. The administration of golden sea cucumber (Stichopus hermanii) extract on rats with hyperglycemia does not reduce blood sugar level, but the extract administration increases the number of pancreatic islet. Golden sea cucumber (Stichopus hermanni) extract may improve the number of pancreatic islet and prevent the progressive damage of pancreatic islet.C

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

The Effect of Apis mellifera Propolis on RUNX-2 and ALP during Remodeling of Orthodontic Tooth movement

2021 ◽  
pp. 2363-2366
Author(s):  
Budi Handayani ◽  
Mieke Sylvia Margaretha A R ◽  
Rini Devijanti Ridwan ◽  
Mohammed Aljunaid
Keyword(s):  
Apis Mellifera ◽  
Immunohistochemical Staining ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Laboratory Research ◽  
Control Group ◽  
Propolis Extract ◽  
Treatment Groups ◽  
Experimental Laboratory ◽  
Group 2

Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis mellifera bees has a very useful composition whose active ingredients are flavonoids, CAPE and saponin. This research aims to analyze the effect of Apis mellifera propolis on RUNX-2 and ALP during the remodeling period caused by the orthodontic tooth movement in Cavia cobaya. It constitutes an experimental laboratory study and a random sampling method. The method used experimental laboratory research on 28 Cavia cobaya, which were divided into four groups consisting of two control groups and two treatment groups which have been given 3% and 5% propolis extract respectively. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Materials and Methods: Propolis is a resin substance produced by bees. Cavia cobaya with orthodontic tooth movement by separator rubber. The control group (Group 1) included clinically healthy Cavia cobaya (n=7), (Group 2) included Cavia cobaya with orthodontic tooth movement (n=7). A treatment group (Group P1) Cavia cobaya have been treated with orthodontic tooth movement and propolis 3%, (Group P2) Cavia cobaya have been treated with orthodontic tooth movement and propolis 5% and observed for 17 days. Samples of maxilla were taken from the subjects for analysis on day 17. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Results: showed that RUNX-2 and ALP expression were found to increase in the tension side. Conclusion: Propolis extract at 5% concentration had ability in bone remodeling by increasing RUNX-2 and ALP expression in the tension area during orthodontic tooth movement.

Comparison of ameliorative effects of Taraxacum syriacum and N-acetylcysteine against acetaminophen-induced oxidative stress in rat liver and kidney

2020 ◽  
Author(s):  
Reza Eshrati ◽  
Mahvash Jafari ◽  
Saeed Gudarzi ◽  
Afshen Nazari ◽  
Esmaeil Samizadeh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Control Group ◽  
Standard Drug ◽  
Ameliorative Effect ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Serum Biochemical

Abstract Taraxacum syriacum (TS) with natural antioxidant and pharmacological activities may be considered for treatment of oxidative stress induced by acetaminophen (APAP). The aim of this study was to evaluate the ameliorative effects of the ethanol extract of TS root against hepatorenal toxicity induced by APAP in comparison to N-acetylcysteine (NAC) as a standard drug. Thirty male Wistar rats were randomly divided into five groups. Control group; APAP (1 g/kg) group; APAP–NAC (160 mg/kg) group and APAP-TS100 and APAP-TS200 groups: APAP plus 100 and 200 mg/kg of TS extract, respectively. After 7 days treatment, serum and liver and kidney tissues were prepared and evaluated. TS extract ameliorated the increased lipid peroxidation level and decreased antioxidant enzymes activities and glutathione level in liver and kidney of APAP-treated rats. Moreover, treatment with the TS extract caused significant reduction in the histopathological damages and high levels of serum biochemical markers of hepatic and renal functions after APAP treatment. This study suggests that the extract of TS roots has dose-dependent ameliorative effect against APAP-induced oxidative damage in liver and kidney due to its free radical scavenging and antioxidant properties. The overall efficacy of the extract at 200 mg/kg dose is comparable with NAC.

scholarly journals Pengaruh Kontrasepsi Suntik DMPA Terhadap Kenaikan Berat Badan Tikus Betina Galur Wistar

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Dwi Retna Prihati
Keyword(s):  
Body Weight ◽  
Test Group ◽  
Laboratory Research ◽  
Control Group ◽  
Female Rat ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test ◽  
Experimental Laboratory ◽  
Rat Strain

Abstract: DMPA Injectable Contraception, Body Weight. The purpose of this study to determine the effect of contraception DMPA injection to BB in female blood wistar strain female. The benefits of this study are expected to increase public knowledge about injectable contraception especially related to the side effects of weight gain. This type of research is an experimental laboratory. Research design Randomized pre-post test group with control, the sample size of 10 adult female mice Wistar strain divided into 2 groups. Data were analyzed by independent T test. Significant value of p <0.05. The result of this research is there is significant difference between control group and DMPA contraception treatment group (p = 0,008). In conclusion contraceptive DMPA affects body weight wistar female rat strain.

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