scholarly journals Pengaruh Spirulina terhadap Kadar beta hCG Tikus Bunting yang Diinduksi Interleukin-6 (Studi Bahan Biologis Tersimpan)

2021 ◽  
Vol 10 (1) ◽  
pp. 84
Author(s):  
Harry K Gondo ◽  
Elizabeth Haryanti
Keyword(s):  
Interleukin 6 ◽  
Potential Effect ◽  
Control Group ◽  
Antioxidant Vitamin ◽  
Control Group Design ◽  
Pregnant Rats ◽  
Beta Hcg ◽  
Post Test ◽  
Experimental Laboratory ◽  
Β Hcg

Preeclampsia is multisystem specific disorder in pregnancy. Preeclampsia characterized by increased cytokine Interleukin-6 and β hCG (human Chorionic Gonadotropin). Spirulina is green-blue alga contain antioxidant, vitamin, mineral. Spirulina has a potential effect as antiinflammation. The aim of this research was to know effect of spirulina to repair trophoblast at β hCG level of pregnant rats with preeclampsia condition (biology material collecting). The type of this research is an experimental laboratory with post-test only control group design. Preeclampsia models induced by Interleukin-6. Twenty-five rats were grouped to five group: Control, Control Positive, Spirulina dose 10, 20, and 40 mg/day, at the end of treatment, β hCG level were analyzed. Statistical analysis was used by one-way ANOVA. The results of this study showed that β hCG level of group spirulina dose 10 mg/day (85.11 ± 25.70 mIU/ml) did not significantly different (p=0.353), with group Dose 20 mg/day (79.65 ± 10.65 mIU/ml). Level of β hCG in Spirulina group dose 40 mg/day were 93.28 ± 17.12 mIU/ml. The spirulina groups dose 10 mg/day and dose 40 mg/day did not show significantly different level of β hCG (0.730> 0.05). Administration of spirulina at a dose of 10 mg was able to significantly reduce levels of β hCG (P <0.05) than at doses of 20 mg / day and 40 mg/ day.

scholarly journals PERBAIKAN FUNGSI TROFOBLAST PADA KADAR β hCG TIKUS BUNTING PREEKLAMPSIA PADA BAHAN BIOLOGIS TERSIMPAN PASCA PERLKUAN DENGAN SPIRULINA

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Adhiono , A ◽  
Gondo H K ◽  
Noer Kumala I
Keyword(s):  
Interleukin 6 ◽  
Dose Group ◽  
Bioactive Compound ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Antioxidant Vitamin ◽  
Control Group Design ◽  
Post Test ◽  
Β Hcg ◽  
Level Group

Abstract: Preeclampsia is multisystem specific disorder in pregnancy. Preeclampsia has signed byincreased cytokine Interleukin-6 and β hCG (human Chorionic Gonadotropin). Spirulina is green-bluealga has bioactive compound namely antioxidant, vitamin, mineral. And has benefit namelyantinflamation. The aim of this research was to know how spirulina influence to repair trophoblast inHcg concentration on rat pregnancy (biology material collecting). The type of this research is anexperimental laboratoric with post test only control group design. A total of 25 rats with pre-eclampsiamodels induced by Interleukin 6 are divided into 5 groups: the untreated control group, the positivecontrol group with Interleukin 6 induction for three days, the treatment group with a dose of 10 mg /day, 20 mg / day and 40 mg of spirulina. / day for five day, then the blood serum produced was measuredwith β hCG levels using ELISA (Enzyme-Linked Immunosorbent Assay). The results of the Spirulina 10mg / day are higher at 85.11 ± 25.70 mIU / ml from a dose of spirulina 20 mg / day at 79.65 ± 10.65mIU / ml . In the level of β hCG, the dose group of spirulina 10 mg / day and the group there is nosignificant difference (0.730> 0.05), the β hCG level group of spirulina 40 mg / day was 93.28 ± 17, 12mIU / ml from the dose group of spirulina 10 mg / day was 85.11 ± 25.70 mIU / ml. The administrationof spirulina for five days was able to reduce β hCG levels at a dose of 10 mg / day, 20 mg / day and 40mg / day and the dose that was most effective in reducing β hCG levels significantly (P <0.05) was adose of 10 mg than dose of 20 mg / day and 40 mg / day.

scholarly journals The potential effect of pandan wangi leaf “pandanus amaryllifolius roxb.’’ extract from Indonesia as time sleep inductor

2019 ◽  
pp. 5-7
Author(s):  
Nita Tri Sulistiyati ◽  
Riandini Aisyah ◽  
EM Sutrisna
Keyword(s):  
Sleep Disturbances ◽  
Leaf Extract ◽  
Traffic Accidents ◽  
Sleep Onset ◽  
Potential Effect ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Experimental Laboratory ◽  
Pandanus Amaryllifolius

Sleep plays a role in restoring the body's biochemical and physiological processes which decrease when awake. People is not familiar yet with sleep disturbances, so they rarely seek help. Sleep disturbances can cause family problems and are at risk of fatal traffic accidents. Fragant Pandan leaves (Pandanus amaryllifolius Roxb.) contains alkaloids, saponins and flavonoids which can provide a hypnotic sedative effect. The purpose of this study was to determine the effect of fragant Pandan leaf extract (Pandanus amaryllifolius Roxb.) administration in shortening the sleep induction time of male Swiss Webster strain mice. A post-test only design experimental laboratory study was conducted with control group design. The test animals used were 25 Swiss Webster male mice divided into five groups randomly : G1 group was given distilled water, G2 was given diazepam dose 1 mg / KgBB, the G3, G4 and G5 groups were given fragant Pandan leaf extract with a dose of 4 mg/25g BB, 6 mg/25g BB and 8mg/25g BB, respectively. One Way ANOVA statistical test showed significant differences in mice sleep onset (p=0.000) between groups. The results of the Post Hoc test using LSD analysis showed that there were significant differences between positive controls with groups G3, G4 and G5. Fragrant Pandan leaf extract dose of 8 mg/g BB can shorten the time of mice sleep induction male Swiss Webster strain mice. While the dose of 4 mg/g BB cannot shorten the time of induction of sleeping mice. Keywords: Fragant Pandan leaf extract; Hypnotic-sedative; Sleep disturbances.

scholarly journals Gambaran Histopatologi Pankreas Tikus Putih (Rattus norvegicus) Pasca Perlakuan Iskemia-Reperfusi Ginjal

2019 ◽  
Vol 13 (1) ◽  
pp. 58
Author(s):  
Elva Rosiana ◽  
Wiwit Ade Fidiawati ◽  
Darmawi Darmawi
Keyword(s):  
Tissue Damage ◽  
Control Treatment ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
White Rats ◽  
Post Test ◽  
Experimental Laboratory ◽  
Histopathologic Feature

Ischemic-reperfusion injury is defined by a condition of hypoperfusion in the spesific organ followed by reperfusion(reoxygenation) inducing tissue damage. Tissue damage produces reactive oxygen species (ROS) leading to oxidativestress condition. Oxidative stress mediate the lipid peroxidation reactions, harm the cell and finally facilitate the celldeath. The aim of this study was to evaluate the histopathologic feature of white rat’s pancreas post renal ischemiareperfusion.This study was an experimental laboratory research with post-test-only control group design including 20male white rats. Rats were divided into 5 groups, control, treatment 1 (45 minutes ischemia), treatment 2 (45 minutesischemia followed by 1 hour reperfusion), treatment 3 (45 minutes ischemia followed by 2 hours reperfusion) andtreatment 4 (45 minutes ischemia followed by24 hours reperfusion). We found the change of the histopathologicalcharacteristics of white rat’s pancreas in term of edema, leukocyte infiltration and vacuolization.

scholarly journals JUS SEMANGKA MENURUNKAN NEUTROFIL TIKUS JANTAN GALUR WISTAR YANG TERPAPAR ASAP ROKOK

2018 ◽  
Vol 11 (2) ◽  
pp. 166
Author(s):  
Rizky Prihandari ◽  
Lailatul Muniroh
Keyword(s):  
Cigarette Smoke ◽  
Hazardous Substances ◽  
Male Rats ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Post Test ◽  
Experimental Laboratory

Cigarette smoke contains many hazardous substances and free radical which affect respiratory system. Watermelon “Sugar Baby” juice contain lycopene as antiinfl ammation. The aim of this research was to analyze the effect of watermelon as antiinfl ammation toward neutrophils count in wistar strain male rats exposed by  cigarette smoke. This study was an experimental laboratory research with pre and post test control group design. Sample consisted of 24 wistar strain male rats with ±150-230 grams weight and 3 months old which were divided into four groups. Control group was given placebo (aquadest) and treatment groups were given w atermelon juice with terraced quantity (1.5 ml, 2.5 ml, and 3.5 ml). Every group was given two sticks of cigarette smoked per day during two weeks then smoked with treatment during one week. Neutrophil from peripheral smear was observed by manual method and statistically analyzed using Anova Mixed Design and continued with One Way Anova. The result showed signifi cant increased of  neutrophil at second observation on control group (p=0.002) and treatment groups (p=0.000). Signifi cance decreased also found in the treatment groups’s at third observation p=0.000. Signifi cant between each group p= 0.017. There was signifi cant different between control and treatment groups but there was no signifi cant different between each treatment groups. This study concluded that there was decreased of  neutrophil in wistar strain male rats which were given w atermelon juice 1.5 ml, 2.5 ml, and 3.5 ml during infl ammation. 3.5 ml w atermelon juice which were given showed better result as antiinfl ammation..

scholarly journals Omega-3 and Vitamin E Supplementation Effect to Rattus Norvegicus Placental Apoptosis Marker: Pre-Eclampsia Model

2021 ◽  
Vol 6 (4) ◽  
pp. 261-268
Author(s):  
Yesi Gusti ◽  
Arni Amir ◽  
Gusti Revilla
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Placental Tissue ◽  
Control Group ◽  
Omega 3 ◽  
Control Group Design ◽  
Pregnant Rats ◽  
Post Test ◽  
The Mean ◽  
Animal House

Background: Pre-eclampsia has been associated with the increased of placental apoptosis caused by oxidative stress. Omega-3 fatty acids and vitamin E had beneficial function to maintain cell membranes, prevent oxidative stress and inhibit the production of proinflammatory cytokines. The purpose of the study was to determine the effect of omega-3 and vitamin E supplement to BCL-2 and BAX on PE rats model. Methods: This research has been worked out at animal house and Biomedical Laboratory of the Medical Faculty Andalas University. The design of this research was experimental study with post test only control group design. There were 35 pregnant rats were divided into 5 groups. Group K- without treatment, group K+ was given L-NAME, group P1 was given L-NAME + omega-3, group P2 was given L-NAME + vitamin E and group P3 was given L-NAME + omega-3 + vitamin E. The L-NAME dose was 50mg/kg/day, At the 19th day of gestation, the pregnancy was terminated and the placental tissue was collected for examining BCL-2 and BAX levels by using ELISA kit. The data were analyzed by one way ANOVA and post hoc test LSD. Results: The mean levels of BCL-2 in each group were K- =62.49 ng/mg, K+ =50.64 ng/mg, P1 =58.79 ng/mg, P2 =53.70 ng/mg and P3 =70.10 ng/mg. The mean levels of BAX were K- =72.06 ng/mg, K+ =78.61 ng/mg, P1 =73.71 ng/mg, P2 =74.31 ng/mg and P3 =71.63 ng/mg. The analysis results concluded that there were significant differences between omega-3 and vitamin E supplements on BCL-2 levels (p=0.000) and BAX levels (p=0.001). Conclusion: Omega-3 (DHA 120 mg, EPA 180 mg) and vitamin E (α-tocopherol 300 iu) suplement increased BCL-2 level and decreased BAX on pre-eclampsia rats model. Keywords: Omega-3, Vitamin E, BCL-2, BAX, Pre-eclampsia.

scholarly journals TINGKAT KEKERASAN PERMUKAAN RESIN KOMPOSIT AKIBAT MASA KEDALUWARSA MATERIAL

2018 ◽  
Vol 5 (1) ◽  
pp. 45
Author(s):  
Yuvika Intan Ristian Putri ◽  
Muhammad Dian Firdausy ◽  
Niluh Ringga Woroprobosari
Keyword(s):  
Surface Hardness ◽  
Composite Resins ◽  
Control Group ◽  
Expiration Date ◽  
Control Group Design ◽  
Expiry Date ◽  
Post Test ◽  
Experimental Laboratory ◽  
Post Hoc ◽  
One Way Anova

Background: Aplication of composites resin in relatively small amount as a restorative material often causing the material reaches its expiry date before all of the material can be used. Composites resin that have passed the expiry date would affect charateristic of the material. The aim of this study was to investigate surface hardness difference of composite resins based on material expiration date.Methods: This research was an experimental laboratory using post test only with control group design. Samples for this research were 27 divided into 3 groups based on its expiration date (2016, 2018 and 2019). The surface hardness was measured by Shore D Hardness. The result of this research were tested with One Way Anova and Post Hoc test LSD.Result: The lowest surface hardness result was measured on group 1 (81.02SHD) and the highest was group 3 (94.72 SHD). The result of One Way Anova test showed that there was signifcant differences in all groups (p<0.05). Post Hoc LSD test showed difference signifcantly between each groups.Conclusion: It can be concluded that there was signifcant difference of composite resins surface hardness based on material’s expiration date

scholarly journals Inhibitory Power of Papaya Leaves to the Adhesion of Streptococcus Mutans Bacteria to Neutrophils

2021 ◽  
Vol 14 (3) ◽  
pp. 1381-1387
Author(s):  
Nining Kurniati
Keyword(s):  
Streptococcus Mutans ◽  
Control Group ◽  
Mean Square ◽  
Control Group Design ◽  
Minimum Concentration ◽  
Post Test ◽  
Experimental Laboratory ◽  
Inhibitory Power ◽  
Adhesion Index

Papaya leaves contain several active substances that are thought to inhibit bacterial adhesion. This study aims to determine the inhibitory power of papaya leaf extraction and papaya leaf infusion to the adhesion of Streptococcus mutans bacteria to neutrophils and differences in inhibition in various concentrations. The method used is an in vitro experimental laboratory with a post-test-only control group design. The sample consisted of several concentrations of papaya leaf extraction and papaya leaf infusion (control, 25%, 50%, 75%, and 100%). The treatment group was added with neutrophil isolates and incubated for 3 hours, then exposed to Streptococcus mutans for 2.5 hours. Knowing the adhesion index can be done by calculating the average number of Streptococcus mutans attached to 100 neutrophils. The results showed successively with rounding in control, 25%, 50%, 75%, 100% papaya leaf infusion, adhesion of 9237, 8929, 7436, 3870, 3162 bacteria to neutrophils occurred. The minimum concentration of 25% papaya leaf extraction and 50% papaya leaf infusion can inhibit the adhesion of Streptococcus mutans to neutrophils. There is a difference in the ability between papaya leaf extraction and papaya leaf infusion in inhibiting the adhesion of Streptococcus mutans to neutrophils as follows: papaya leaf extraction 25% to papaya leaf infusion 25% obtained sig value ≥0.001* with a mean square of -16.38, papaya leaf extraction 50% to Papaya leaf infusion 50% got a sig value of ≥0.001* with a mean square of -42.91, 75% papaya leaf extraction to 75% papaya leaf infusion got a sig value of ≥0.001* with a mean square of -28.85 and 100% papaya leaf extraction to 100% papaya leaf infusion obtained a sig value of ≥0.001* with a mean square of -26.11.

scholarly journals Pengaruh Spirulina terhadap VEGF dari Trofoblast Tikus Putih Hamil Strain Wistar yang Diinduksi Interleukin 6

2020 ◽  
Vol 9 (2) ◽  
pp. 123
Author(s):  
Harry K Gondo ◽  
Elizabeth Haryanti
Keyword(s):  
Interleukin 6 ◽  
Positive Control ◽  
Control Group ◽  
Vascular Endothelial ◽  
Control Group Design ◽  
Blue Green Algae ◽  
White Rats ◽  
Post Test ◽  
Endothelial Growth Factor ◽  
Vegf Level

Preeclampsia is a pregnancy disorder with hypertension as one of its common symptoms. Preeclampsia in pregnancy is characterized by increasing of proteinuria, blood pressure and also interleukin 6. Spirulina sp is a thread like shaped blue-green algae, similar to chain of cylindrical cells with 1 to 12 μm diameter of cell membrane. The purpose of this research was to know the effect of spirulina on VEGF level in improving of trophoblast function in pregnant white Rats Wistar. The design of the study was laboratory experimental with post-test only control group design. Twenty-five rats with preeclampsia model induced by interleukin 6, were divided into 5 groups. The groups were control group, positive control group (P0) injected by interleukin-6 dose of 5 ng/ 100 gram body weight (BW), group with spirulina dose of 10 mg/100 gram BW (P1), group with spirulina dose of 20 mg/100 gram BW (P2); and group with spirulina dosage 40 mg/100 gram BW (P3). The Vascular endothelial growth factor (VEGF) levels were in experimental groups measured compared to control. The averages of group spirulina were 81.31 (10 mg/100 gram BW), 65.99 (20 mg/100 gram BW), and 49.62 (40 mg/100 gram BW). In comparison with control group (62.70), VEGF level in group administered by spirulina dose of 20 mg/100 gram BW were close to control group.

scholarly journals Pengaruh Ekstrak Kulit Buah Naga Merah (Hylocereus Polyrhizus) Terhadap Kadar Interleukin-6 Mencit Model Endometriosis

2018 ◽  
Vol 19 (3) ◽  
pp. 197
Author(s):  
Sagita Candra Puspitasari
Keyword(s):  
Interleukin 6 ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Hylocereus Polyrhizus

ABSTRAK Pada endometriosis terjadi proses inflamasi lokal pada pelvis dengan perubahan fungsi sel-sel imun di lingkungan peritoneum. Respon imun pada zalir peritoneum endometriosis berupa peningkatan aktivitas makrofag dan terjadinya sekresi berlebihan beberapa sikotin seperti IL-1, IL-6, IL-8, dan TNF-⍺ sehingga menyebabkan aktivasi faktor transkripsi NF-KB. Buah naga merah merupakan salah satu tanaman yang mampu menghambat sekresi sitotin NF-κB dari endometriosis. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak buah naga merah terhadap kadar Interleukin-6 mencit model endometriosis. Sampel 30 mencit betina dibagi menjadi 5 kelompok yang terdiri dari kelompok kontrol positif, kelompok kontrol negatif dan kelompok perlakuan dosis bertingkat. Kontrol positif dan kelompok perlakuan dijadikan endometriosis selama 14 hari, kemudian 14 hari berikutnya kelompok positif dan kelompok negatif diberi larutan Na-CMC 0,5%, sedangkan kelompok perlakuan diberi dosis bertingkat. Jenis penelitian ini true eksperimental dengan menggunakan rancangan randomized post test only control group design. Hasil penelitian ini menunjukkan bahwa terdapat perbedaan yang signifikan pada kadar Interleukin-6 antar kelompok. Pemberian ekstrak kulit buah naga pada mencit model endometriosis dapat menekan kadar Interleukin-6 dengan hasil signifikan p<0,05.  Kata kunci : ekstrak kulit buah hylocereus polyrhizus, endometriosis, IL-6,

scholarly journals SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT ON MALE WHITE RATS KIDNEY NECROSIS

2017 ◽  
Vol 52 (1) ◽  
pp. 62
Author(s):  
Ardy Hamonangan Naibaho ◽  
Arifa Mustika ◽  
Anny Setijo Rahaju
Keyword(s):  
Treatment Group ◽  
Ethanol Extract ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Post Test ◽  
Experimental Laboratory ◽  
Group 2 ◽  
High Doses

Singawalang leaf is used as a traditional medicine by the communities suffering from pneumonia due to TB disease. However, experiments to explain it’s toxicity are few. Therefore, this study was conducted to disclose it’s toxicity. This was an experimental laboratory study using post–test only control group design. The sampling method was simple random sampling. The animals were randomly divided into 4 (four) groups, each group consisted of 8 (eight) mice, the control group (P0) received distilled water as a solvent material. Treatment group 1 (P1) obtained ethanol extract of singawalang (Petiveria alliaceae) in low doses of 90 mg/kgbw. Treatment group 2 (P2) obtained ethanol extract of singawalang (Petiveria alliaceae) in medium doses of 180 mg/kgbw. Treatment group 3 (P3) obtained ethanol extract of singawalang (Petiveria alliaceae) in high doses of 360 mg/kgbw. Th extracts were given with sonde once a day for 30 days. Results showed that the leaf ethanol extract singawalang (Petiveria alliaceae) does not induce sub-acute necrosis effects on kidney of male white mice (Mus musculus) in doses 90 mg/kgbw, 180 mg/kgbw, and 360 mg/kgbw.

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