Aspects of applying the method of chemical mutagenesis to develop cultivars of Calendula officinalis L

2021 ◽  
Vol 182 (1) ◽  
pp. 110-122
Author(s):  
F. M. Khazieva ◽  
I. N. Korotkikh
Keyword(s):  
Seed Quality ◽  
Raw Materials ◽  
Morphological Characteristics ◽  
Mutation Breeding ◽  
Chemical Mutagenesis ◽  
Low Grade ◽  
New Varieties ◽  
Number Of Leaves ◽  
Total Extractives ◽  
Calendula Officinalis L

Background. The need for continuous breeding practice to develop new varieties of Calendula officinalisL. is induced by the accumulation of low-grade morphotypes in the already existing cultivars as a result of crosspollination.Materials and methods. Seeds selected from high-yielding biotypes of cv. ‘Kalta’ were treated with various mutagens for 18 hours, using 1000 seeds for each option. The mutation rate in the M1 generation and seed quality were determined, and plant viability was assessed under field conditions. Plant height, number of branches, number of leaves, number of inflorescences per plant, inflorescence diameter, number of ray floret rows, and weight of one inflorescence were measured in the mass flowering phase. The yield of raw materials (fresh and air-dry biomass of inflorescences) was calculated for each harvest and for the total amount of all 7 harvests during the flowering period.Results.The mutagens DES 0,05% and DMS 0,08% turned outthe most effective for developing new source material. None of the plants produced seeds after treatment with nitrosomethyl urea (NMU0.02% ), and only one plant produced seeds after treatment with NMU 0.04% . As a result of a long-term breeding process (2009–2016), new varieties were developed on the basis of mutant generations M2–M3 : cvs. ‘Zolotoe more’ and ‘Rajskij sad’, both exceeding cv. ‘Kalta’ in the yield of inflorescences by 39 and 30%, seed yield by 48 and 49%, total extractives by 13.8 and 21.3%, and total flavonoids by 29.9% and 43.3%, respectively.Conclusion.New cultivars of C. officinalis, ‘Zolotoe more’ and ‘Rajskij sad’, were developed in accordance with the mutation breeding scheme that employed methods of chemical mutagenesis, with expanded selection of plants altered in their morphological characteristics, productivity and content of bioactive compounds in the first mutant generation and assessment of uniformity, distinctness and stability in the second and subsequent generations.

scholarly journals Obtaining a concentrated sugar syrup from cell juice of new sugar sorghum varieties

2021 ◽  
pp. 178-181
Author(s):  
Е.С. Романенко ◽  
Е.А. Миронова ◽  
Т.С. Айсанов ◽  
М.В. Селиванова ◽  
Н. А. Есаулко ◽  
...  
Keyword(s):  
Raw Materials ◽  
Sugar Content ◽  
Morphological Characteristics ◽  
Raw Material ◽  
Processing Plant ◽  
Plant Raw Materials ◽  
The North ◽  
Sugar Syrup ◽  
New Varieties ◽  
Materials Used

Представлены результаты исследования новых линий сортов сахарного сорго селекции ФГБНУ «Северо-Кавказский федеральный научный аграрный центр» для создания качественно новой комплексной безотходной технологии переработки экологически безопасной продукции растениеводства для производства функциональных напитков с улучшенными потребительскими свойствами. В зависимости от вида используемого сырья, можно получить новые интересные вкусовые характеристики напитка. В качестве натуральных ингредиентов использовали нетрадиционное растительное сырье - сахарное сорго. В статье представлены данные по морфологическим признакам и показатели содержания сахаров в соке стеблей новых линий сахарного сорго: Л.7812 (57), Л.8611 (58), Л.7813 (56), Л.7859 (60), Ларец 63 и расчетный выход сиропа с 1 га посева. Авторами подробно описана технология переработки растительного сырья и получения концентрированного сиропа. Впервые в практике научных исследований разработана комплексная, безотходная технология переработки сахарного сорго для производства концентрированного сахарного сиропа и дальнейшего его использования с целью получения функциональных напитков с улучшенными функциональными потребительскими свойствами. Актуальность работы и научно-техническая значимость состоит в подборе новых сортов сахарного сорго, технологии выделения клеточного сока из стеблей и приготовления концентрированного сахарного сиропа. Таким образом, на основании проведенных исследований были получены результаты, которые представляют интерес для использования сахарного сорго в сельскохозяйственном производстве (для корма животных и перерабатывающей промышленности, для производства продуктов питания, в том числе функционального назначения). Вся информация представлена в табличном виде с разделением изучаемых сортов, указаны основные морфологические признаки и показатели содержания сахаров в соке стеблей сортов сахарного сорго и расчетный выход сиропа с 1 га. This paper provides information about research on new lines of sugar sorghum varieties selected by the North Caucasian Federal Scientific Agrarian Center to create a brand new, innovative, integrated, waste-free technology for processing environmentally friendly crop products for the production of functional drinks with improved consumer properties. Depending on the type of raw materials used, you can get completely new interesting flavor characteristics of the drink. In the study, an unconventional plant raw material, sugar sorghum, was used as a natural ingredient. The paper presents data on morphological characteristics and indicators of sugar content in the juice of stems of new lines of sugar sorghum: L. 7812 (57), L. 8611 (58), L. 7813 (56), L. 7859 (60), Larets 63 and estimated yield of syrup from 1 hectare of sowing. The authors of the article described in details the technologies for processing plant raw materials and obtaining concentrated syrup. For the first time in the practice of scientific research, a comprehensive, waste-free technology for processing sugar sorghum has been developed for the production of concentrated sugar syrup and its further use for functional drinks with improved functional consumer properties. The relevance of the work and the scientific and technical significance lies in the selection of new varieties of sugar sorghum, technology for isolation of cell juice from stems of sugar sorghum and preparation of concentrated sugar syrup. Thus, on the basis of the studies carried out, promising results for the use of sugar sorghum in agricultural production for animal forage and the processing industry for food production, including functional purposes, were obtained. All information is presented in tabular form with the division of the studied varieties. Main morphological signs and indicators of sugar content in the juice of stems of sugar sorghum varieties and the estimated yield of syrup per hectare are indicated.

scholarly journals Exogenous 5-azaCitidine accelerates flowering and external GA3 increases ornamental value in Iranian Anemone accessions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vahideh Yari ◽  
Zeynab Roein ◽  
Atefeh Sabouri
Keyword(s):  
Morphological Characteristics ◽  
Flower Longevity ◽  
Cut Flowers ◽  
Stem Height ◽  
Flowering Stage ◽  
Potted Plants ◽  
Dormancy Period ◽  
Treatment Conditions ◽  
Number Of Leaves ◽  
Pre Treatment

AbstractThe Anemone genus is a tuberous geophyte which undergoes a dormancy period during unfavorable environmental conditions for growth. Five species of the Anemone genus naturally grow in several regions of Iran. The diverse uses of Anemone in gardens for landscaping, cut flowers, and potted plants indicate its high ornamental potential. Its dormancy and flowering are influenced by various factors. The present paper was conducted to explore the flowering behavior of Anemone accessions in response to different pre-treatments. For this purpose, tubers of 18 Anemone accessions (A. coronaria and A. biflora) were collected from natural regions of six provinces in Iran. These tubers were subjected to different conditions of non-chilling (20 °C, 90 days), chilling (4 °C, 90 days), GA3 (150 mgL-1; 24 h), and 5-azaCitidine (5-azaC; 40 µM; 24 h) prior to the cultivation. Most of the accessions were able to enter the flowering stage without chilling. The shortest period for the sprouting of tubers (16.89 ± 7.83 days) belonged to 5-azaC pre-treatment. In addition, this treatment accelerated the flowering time (about 30 days earlier) and diameter of the stem, bud, and flower. Morphological characteristics, such as stem height, number of leaves, bud, and petal and the longevity of flowers on the plant were significantly affected by GA3 pre-treatment. Our results indicated a positive correlation between flower length, stem height, and stem diameter with flower longevity under different pre-treatment conditions. The present study demonstrated that accessions Anm3, Anm12, and Anm18 had ornamental values higher than the population mean across four conditions.

scholarly journals Mineralogical Characterization and Firing Temperature Delineation on Minoan Pottery, Focusing on the Application of Micro-Raman Spectroscopy

Heritage ◽  
2019 ◽  
Vol 2 (3) ◽  
pp. 2652-2664 ◽  
Author(s):  
E. Grammatikakis ◽  
Kyriakidis ◽  
D. Demadis ◽  
Cabeza Diaz ◽  
Leon-Reina
Keyword(s):  
Bronze Age ◽  
Firing Temperature ◽  
Raw Materials ◽  
Morphological Characteristics ◽  
Ceramic Technology ◽  
Phase Identification ◽  
Mineralogical Characterization ◽  
Analytical Protocol ◽  
Crucial Information ◽  
Analytical Tools

Ceramic objects in whole or in fragments usually account for the majority of findings in an archaeological excavation. Thus, through examination of the values these items bear, it is possible to extract important information regarding raw materials provenance and ceramic technology. For this purpose, either traditional examination protocols could be followed, focusing on the macroscopic/morphological characteristics of the ancient object, or more sophisticated physicochemical techniques are employed. Nevertheless, there are cases where, due to the uniqueness and the significance of an object of archaeological value, sampling is impossible. Then, the available analytical tools are extremely limited, especially when molecular information and mineral phase identification is required. In this context, the results acquired from a multiphase clay ceramic dated on Early Neopalatioal period ΜΜΙΙΙΑLMIA (1750 B.C.E.–1490 B.C.E.), from the Minoan Bronze Age site at Philioremos (Crete, Greece) through the application of Raman confocal spectroscopy, a nondestructive/ noninvasive method are reported. The spectroscopic results are confirmed through the application of Xray microdiffraction and scanning electron microscopy coupled with energy dispersive Xray spectrometry. Moreover, it is demonstrated how it is made possible through the application of microRaman (μRaman) spectroscopy to examine and collect crucial information from very small inclusions in the ceramic fabric. The aim of this approach is to develop an analytical protocol based on μRaman spectroscopy, for extracting firing temperature information from other ceramic finds (figurines) where due to their uniqueness sampling and analyses through other techniques is not possible. This information can lead to dating but also to firing kiln technology extrapolations that are very significant in archaeology.

Research on Properties of Low Grade Cement-Based Composite Materials Modified by Silica Fume

2012 ◽  
Vol 174-177 ◽  
pp. 751-756
Author(s):  
Zi Fang Xu ◽  
Ming Xu Zhang ◽  
Jin Hua Li
Keyword(s):  
Silica Fume ◽  
Mechanical Performance ◽  
Raw Materials ◽  
Cement Matrix ◽  
Hardened Cement ◽  
Low Grade ◽  
X Ray ◽  
Matrix Microstructure ◽  
Nano Powder ◽  
Strength And Durability

In order to notably improve the mechanical properties and durability of low-grade cement-based material, superfine silica fume was used to modify the cement-based composite based on special perfomance and effects of nano powder. The mechanical performance and durability were investigated.Then the phase compositions,microstructure and morphologies of as-received cement-based composite were studied by X-ray Diffractometer、TGA-DTA and SEM. The results show that： the best formula of raw materials is 1:1:0.025:0.015, and hydration can be accelerated and increasing of hydration products is observed after modification. In the hardened cement matrix, microstructure is very compacted and C-S-H gel forms densed structure, so the structure defect is notably reduced. This means that both strength and durability of cement-based composite are notably improved by the addition of superfine silica fume.

scholarly journals Impact of Sodium Hexametaphosphate on the Flotation of Ultrafine Magnesite from Dolomite-Rich Desliming Tailings

Minerals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 499
Author(s):  
Duong Huu Hoang ◽  
Doreen Ebert ◽  
Robert Möckel ◽  
Martin Rudolph
Keyword(s):  
Ultrafine Particles ◽  
Raw Materials ◽  
Low Cost ◽  
Froth Flotation ◽  
Open Circuit ◽  
Ore Deposits ◽  
Sodium Hexametaphosphate ◽  
Low Grade ◽  
Low Contrast ◽  
The Impact

The depletion of ore deposits, the increasing demand for raw materials, the need to process low-grade, complex and finely disseminated ores, and the reprocessing of tailings are challenges especially for froth flotation separation technologies. Even though they are capable of handling relatively fine grain sizes, the flotation separation of very fine and ultrafine particles faces many problems still. Further, the flotation of low-contrast semi-soluble salt-type minerals with very similar surface properties, many complex interactions between minerals, reagents and dissolved species often result in poor selectivity. This study investigates the flotation beneficiation of ultrafine magnesite rich in dolomite from desliming, currently reported to the tailings. The paper especially focuses on the impact of the depressant sodium hexametaphosphate (SHMP) on the following: (i) the froth properties using dynamic froth analysis (DFA), (ii) the separation between magnesite and dolomite/calcite, and (iii) its effect on the entrainment. As a depressant/dispersant, SHMP has a beneficial impact on the flotation separation between magnesite and dolomite. However, there is a trade-off between grade and recovery, and as well as the dewatering process which needs to be considered. When the SHMP increases from 200 g/t to 700 g/t, the magnesite grade increases from 67% to 77%, while recovery decreases massively, from 80% to 40%. The open circuit with four cleaning stages obtained a concentrate assaying 77.5% magnesite at a recovery of 45.5%. The dolomite content in the concentrate is about 20%, where 80% of dolomite was removed and importantly 98% of the quartz was removed, with only 0.3% of the quartz in the final concentrate. Furthermore, the application of 1-hydroxyethylene-1,1-diphosphonic acid (HEDP) as a more environmentally friendly and low-cost alternative to SHMP is presented and discussed. Using only 350 g/t of HEDP can achieve a similar grade (76.3%), like 700 g/t of SHMP (76.9%), while obtaining a 17% higher magnesite recovery as compared to 700 g/t of SHMP. Interestingly, the proportion of hydrophilic quartz minerals ending up in the concentrate is lower for HEDP, with only 1.9% quartz at a recovery of 21.5% compared to the 2.7% of quartz at a recovery of 24.9% when using SHMP. The paper contributes in general to understanding the complexity of the depressant responses in froth flotation.

Synthesis of Low Viscosity Polydimethylsiloxane Using Low Grade of Octamethylcyclotetrasiloxane

2021 ◽  
Vol 1028 ◽  
pp. 365-370
Author(s):  
Diba G Auliya ◽  
Soni Setiadji ◽  
Zulfi Mofa Agasa ◽  
Fitrilawati ◽  
Norman Syakir ◽  
...  
Keyword(s):  
Ring Opening ◽  
Raw Materials ◽  
Vitreoretinal Surgery ◽  
Production Costs ◽  
Low Grade ◽  
High Grade ◽  
High Quality ◽  
Low Viscosity ◽  
Polymerization Method

Polydimethylsiloxane (PDMS) has been widely used as a vitreous humour substitution in vitreoretinal surgery. Due to its limited availability and increasing domestic needs, the price of PDMS in Indonesia became very expensive. Previously, we reported the synthesized of PDMS from a high grade of monomer of 98% of Octamethylcyclotetrasiloxane (D4) and found that all PDMS samples produced high quality samples similar to that of commercial one. However, by considering the ease of obtaining raw materials and also the production costs, the synthesis of PDMS using monomer which easy to be found in Indonesia and low production costs is needed to be developed. Here, we reported the synthesis of PDMS using low grade of 96% of D4 in order to produce high quality of PDMS with low viscosity that can be used for vitreous humous substitution in vitreoretinal surgery. PDMS samples with low viscosity value of 0.94-1.35 Pa.s have been successfully synthesized from low grade of D4 and MM using ring-opening polymerization method. The yields of PDMS resulted in this research were in the range between 67.27% and 76.26%. From FTIR spectroscopy, it is found that all synthesized samples have structure and functional groups similar to PDMS using high grade of monomer of 98% Octamethylcyclotetrasiloxane (D4). From refractometer and surfgauge measurements, all samples have refractive index in the range of 1,4034-1,4040 and the value surface tension was 21 m.N/m.

scholarly journals Optimization of Cultural Conditions for Solid State Fermentation of Amylase Production by Aspergillus species

2013 ◽  
Vol 14 (1) ◽  
pp. 67-74
Author(s):  
Bina Gautam ◽  
Tika B Karki ◽  
Om Prakash Panta
Keyword(s):  
Solid State ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Food Industry ◽  
Raw Materials ◽  
Morphological Characteristics ◽  
Amylolytic Enzyme ◽  
Cultural Conditions ◽  
Amylase Production ◽  
Selection Of

Amylase is an amylolytic enzyme used in food industry which is generally produced by Aspergillus spp. under solid state fermentation. The present study is concerned with the isolation, screening and selection of suitable strains of Aspergillus spp. and optimization of cultural conditions for the biosynthesis of amylase. Rice and wheat brans were used as substrates which are readily available inexpensive raw materials for amylase production. From 85 samples of rice and wheat grains, 55 colonies obtained on potato dextrose agar (PDA) were suspected to be Aspergillus oryzae and only 35 colonies possessed the morphological characteristics similar to that of A. oryzae indicating the isolates were most likely the strains of A. oryzae. Of all the fungal isolates of Aspergillus spps., Asp.31 gave maximum production of amylase (720.782 IUgds-1) in solid state fermentation media. This strain was selected as a parental strain for optimization for cultural conditions. The obtained data were analyzed using SPSS- 11.5 program. Of all the substrates (rice bran, wheat bran and their mixture), rice bran was the best for producing amylase of highest activity 611.614 IUgds-1.The highest enzyme activity of 698.749 IUgds-1 was observed at 50% initial moisture level of the substrate. The optimum temperature was 25°C for producing the crude amylase enzyme with amylase activity of 577.757 IUgds-1. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 67-74 DOI: http://dx.doi.org/10.3126/njst.v14i1.8924

scholarly journals Industrial characteristics of Berberis L. representatives fruits

2018 ◽  
Vol 47 ◽  
pp. 63-69
Author(s):  
Yu. V. Lykholat ◽  
N. O. Khromykh ◽  
T. Yu. Lykholat ◽  
M. I. Nedzvetska ◽  
O. A. Lykholat ◽  
...  
Keyword(s):  
Food Industry ◽  
Seed Quality ◽  
Raw Materials ◽  
Low Frequency ◽  
Botanical Garden ◽  
Raw Material ◽  
Material Base ◽  
Total Antioxidant ◽  
Fruit Plants ◽  
Seed Propagation

The introduction of fruit plants into a culture enriches the diversity of the species composition of the regional flora and at the same time creates an opportunity for expansion of the plant raw material base for the needs of the food industry, inclusion in a full-fledged functional nutrition of the population. Among the fruit plants of the Dniprovsk region, every year, more attention should be paid to introduced low-frequency non-traditional fruit species, in which the fruits, leaves, stems and other parts have high nutritional value and are the source of physiologically active compounds that have anti-cancer, antibacterial and anti-inflammatory properties, carry cardio protective, antihypertensive, anti-diabetic effects, stimulate the central nervous system, etc., and can contribute to the prevention and treatment of many diseases.   Antioxidants additives in the raw materials and finished products ensures their prevention damage, reduce losses, increase shelf life and production of high quality products which keep for a long time characteristics inherent fresh, complete products. Preservation, restoration and introduction to the culture of any species depends primarily on its ability to multiply seed and vegetative methods. A comparative analysis of the fruit and seed ability of Berberis L. representatives grown in the botanical garden of Oles Gonchar’  Dniprovsky National University was carried out. The use of integrated research methods made it possible to carry out an integrated assessment of the regenerative capacity of the investigated representatives of the Berberis L. and to establish their biological ability to seed propagation depending on the seed quality of the seeds. The seeds length varied from 4.5 mm (B. canadensis) to 5.2 mm (B. declinata, B. amurensis), width was from 1.8 mm (B. amurensis, B. vulgares) to 2.1 mm ( B. coreana). The weight of 1000 seeds, which, depending on their size and completeness, amounted to an average of 10.16 grams. Largest weight 1000 pcs. seed is marked in B. coreana. As a result of the total antioxidant ability of the fruits, the species examined can be arranged in the following order:  B. koreana > B. x declinata > B. vulgaris > B. amurensis > B. canadensis. When comparing total antioxidant capacity level of the plants, the highest indices were characteristic for B. koreana and B. x declinata fruits, which exceeded the values of other species in 1.7-1.9 times. The relatively low antioxidants concentration in B. amurensis fruits is offset by a greater weight of the fruits of this species.  In order to enrich the range of ornamental and fruit plants, it is expedient to introduce into production of new crops and to expand the species diversity of Berberis L. representatives. Barberry reproduction process depends on many factors related to the environment. All investigated representatives of the Berberis L. are promising for further use in the planting system and as fruit plants that can be included in the functional nutrition of the population and use in the food industry in the conditions of the Steppe Prydniprovya.

scholarly journals Mutagenic effect of EMS and DES on Tenai (Setaria italica) in M1 generation

1970 ◽  
pp. 06-08
Author(s):  
I. Anittha, L. Mullainathan
Keyword(s):  
Seed Weight ◽  
Quantitative Traits ◽  
Crop Improvement ◽  
Mutagenic Effect ◽  
Mutation Breeding ◽  
Setaria Italica ◽  
Mutagenic Treatment ◽  
Genetic Manipulations ◽  
Number Of Leaves ◽  
Breeding Techniques

Mutation breeding gives better results for crop improvement through genetic manipulations when compared  to  other conventional breeding techniques. The present work focused in order to find out the effect of chemical mutagens; EMS and DES on Setaria italica in M1 generation. The seeds of Tenai, variety CO(Te)7 treated with different concentration of EMS and DES. The LD50 was observed at 30mM in EMS and 40mM in DES. Selection studies were conducted to improve the yield and to generate genetic variability in different quantitative traits such as days to first bloom, plant height, number of leaves, number of nodes, length and breadth of ear head, 1000 seed weight and yield per plant. The results revealed that, all the parameters were decreased with increasing concentration in both EMS and DES, while days to first bloom was increasing with increasing concentration. According to the result all the parameters  studied  shows a negative direction towards crop improvement in M1 generation because of the stress caused by mutagenic treatment.

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