scholarly journals Enhancing the Viability of Rosella Seeds (Hibiscus sabdariffa L.) through Tetrazolium and Paper Media Test

2019 ◽  
Vol 4 (1) ◽  
pp. 23-30
Author(s):  
Taufiq Hidayat RS ◽  
Mayasari Yamin
Keyword(s):  
Seed Viability ◽  
Dry Weight ◽  
Tetrazolium Salt ◽  
Hibiscus Sabdariffa ◽  
Germination Capacity ◽  
Randomized Design ◽  
Vigor Index ◽  
Normal Seedling ◽  
Best Parameter ◽  
Viable Seeds

This study was aimed to determine the viability rate of rosella seed, to obtain the best result of seed testing for enhancing rosella seed viability, to find the best seed invigoration method for enhancing rosella seed viability, to obtain staining pattern through tetrazolium test of rosella seed, and to determine viability and vigor of rosella seed to be further used as estimation indicator for rosella plant growth in the field. The study was conducted in the Seed Laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI), Malang, East Java during July - August 2018. The material used included accessions of rosella seed (Hibiscus sabdariffa L.) ACC. 1148 from the year 2015 and collection of ISFCRI, 100 ml of Tetrazolium solution (40 ml KH2PO4, 60 ml Na2HPO4 and 1 gr of Tetrazolium powder). This research applied Completely Randomized Design (CRD) consisted of seed treatments of control (no immersion/0 hour), immersion for 5 hours, immersion for 10 hours, and scarification, each with 4 replications. Result of this study showed that the use of tetrazolium salt was found to be better in enhancing the viability of rosella seeds. Viable seeds was found to have bright red embryonic axis and bright red cotyledon. Testing using paper media on several seed invigoration treatments resulted in significantly different effect on parameters of vigor index, germination capacity, and dry weight of normal seedling. The best parameter of germination capacity and dry weight of normal seedling was obtained by treatment immersed in water at temperature of 27°C for 10 hours

scholarly journals Identifikasi Mutu Fisik Dan Fisiologis 16 Genotip Benih Calon Tetua Jagung Manis Hibrida Unpad setelah 2 dan 4 Bulan Penyimpanan

2018 ◽  
Vol 6 (1) ◽  
pp. 53
Author(s):  
Debby Yolanda Sulista ◽  
Anne Nuraini ◽  
Muhamad Kadapi
Keyword(s):  
Electrical Conductivity ◽  
Experimental Design ◽  
Seed Quality ◽  
Sweet Corn ◽  
Dry Weight ◽  
Germination Capacity ◽  
Vigor Index ◽  
Seed Growth ◽  
Normal Seedling ◽  
Parental Lines

Hybrid seed is not always have good longetivity. Therefore, breeder should select longetivity character from parental lines to get hybrid with longetivity characeter. Longetivity character can be revealed by physical and physiological identification of seed. The aim of this research was to identify physical and physiological of 16 parental lines genotypes of sweetcorn after storage. The experimental design in this research used Randomized Complete Design. The treatment in this reserach used 16 Unpad sweet corn parental lines with two replications. The data were analyzed using the F test, Scott Knott 5%, and Duncan 5%. The results showed that there were significant differences in seed electrical conductivity, germination capacity, vigor index, normal seedling dry weight and 100 seeds weight , and seed growth simultaneously at 2 month storage, and normal seedling dry weight and 100 seeds weight at 4 month storage. The best genotypes after storage was 578 which was from P parent, according to the delta value of seed quality changed.

scholarly journals Suitability of tetrazolium test for Tamarindus indica L. seeds

2022 ◽  
Vol 43 (2) ◽  
pp. 509-522
Author(s):  
Luciana Nunes Cordeiro ◽  
◽  
Riselane de Lucena Alcântara Bruno ◽  
Lucas Kennedy Silva Lima ◽  
Albericio Pereira de Andrade ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seed Viability ◽  
Dry Weight ◽  
Tetrazolium Salt ◽  
Tamarindus Indica ◽  
Test Protocol ◽  
Randomized Design ◽  
Class 1 ◽  
Set Up ◽  
Tetrazolium Test

Seed quality is routinely assessed by direct tests, e.g, the germination test, or indirect tests like the tetrazolium test, which has shoown to be promising in the determine viability and vigor, allowing the diagnosis of the main problems that may affect seed quality, such as mechanic damages, field deterioration and storage. In this respect, this study was conducted to develop a tetrazolium test protocol to evaluate the viability and vigor of Tamarindus indica L. seeds. Before exposing the seeds to the tetrazolium solution, seed preconditioning studies were carried out in which seven soaking times were tested. The soaking time that did not cause damage to the seed embryo and allowed the removal of the seed coat to expose the seed structures to the tetrazolium salt was selected. Then, an experiment was set up in a completely randomized design with a 2x3x3 factorial arrangement involving two seed lots, three soaking times in tetrazolium salt (6, 12 and 16 h) and three salt concentrations (0.075, 0.1 and 0.5%), totaling 18 treatments with four replicates of 25 seeds, evaluated at 40 ºC. For each treatment, the seeds were divided into three classes, namely, viable and vigorous embryos (class 1); viable embryos (class 2) and non-viable or dead embryos (class 3). For a comparison with the tetrazolium test results, the two seed lots were analyzed for water content, germination, emergence, first count, germination speed index, emergence speed index, growth and seedling dry weight. The viability and vigor of T. indica seeds can be evaluated after a soaking period of 48 h and immersion for 6h in tetrazolium salt at the concentration of 0.1%, at 40°C, with provides results similar to conventional seed viability tests. The tetrazolium test proved to be adequate to differentiate T. indica seed lots in terms of viability.

scholarly journals Influence of seed skin condition, seed drying and concentrations of coconut water on seed viability of papaya seeds (Carica papaya L.)

2019 ◽  
Vol 6 (2) ◽  
pp. 279-286
Author(s):  
Anwar Parhimpunan ◽  
Haryati ◽  
Ferry Ezra T. Sitepu
Keyword(s):  
Seed Treatment ◽  
Block Design ◽  
Seed Viability ◽  
Dry Weight ◽  
Skin Condition ◽  
Coconut Water ◽  
Randomized Block Design ◽  
Vigor Index ◽  
Normal Seedling ◽  
Papaya Seeds

Dormansi seeds was caused on the physic of seeds, embryo physiologic or a combination of those two, it is needed the seed drying and concentrations of coconut water to solved dormancy. The purpose of this research is to know the influence of seed treatment, seed drying and concentrations of coconut water on seed viability of papaya seeds, used randomized block design with three factors. The first factor is seed treatment (sarcotesta and without sarcotesta), the second factor is seed drying (2, 4 and 6 day) and the third factor is concentrations of coconut water (0, 50 and 100 %). The results showed that seeds without sarcotesta increasing seed of water doses, normal seedling percentage, vigor index, seedling fresh weight, seedling dry weight, increased the rate of germination and reducing of not growt seedling percentage. Six days of seed drying significantly affected normal seedling percentage, vigor index and reducing of not growt seedling percentage. Fifty percents of concentration of coconut water significantly affected normal seedling percentage, vigor index and reducing of not growt seedling percentage. There is significantly affected interaction between seeds without sarcotesta and six days of seed drying to the rate of germination. There is significantly affected interaction between seeds without sarcotesta and fifty percents of concentrations of coconut water to seed of water doses. There is significantly affected interaction between seeds without sarcotesta, six days of seed drying and fifty percents of concentrations of coconut water to the rate of germination.

scholarly journals Endogenous Hormones in Habanero Pepper Seeds

2021 ◽  
pp. 9-18
Author(s):  
H. Ramírez ◽  
U. A. Macías-Castillo ◽  
A. I. Melendres-Alvarez ◽  
M. C. Castillo-Robles ◽  
A. Zermeño-González ◽  
...  
Keyword(s):  
Seed Viability ◽  
Dry Weight ◽  
Zeatin Riboside ◽  
Human Society ◽  
Endogenous Hormones ◽  
Vegetable Crops ◽  
Randomized Design ◽  
Habanero Pepper ◽  
Freeze Dried ◽  
Statistical Program

Aims: Habanero pepper (Capsicum annuum L.) cultivation is growing worldwide, mainly as a result of its high demand and nutritive contribution to human society. The presence of endogenous gibberellins and cytokinins in seeds of various vegetable crops has been related to a good germination; however, little is known on habanero pepper. The aim of this study was to search for the presence, identification and amount of gibberellins and cytokinins in seeds of habanero pepper cv “Jaguar”. Study design: Laboratory analysis for gibberellins and cytokinins were organized in solvent solutions groups with three technical replicates using a complete randomized design and results when applicable were analyzed using the statistical program 'RStudio' (version 10) and data obtained subjected to a comparison of means with the Tukey (P≤0.05) test. Place and duration of study: The experiment was conducted at the Department of Horticulture in Universidad Autónoma Agraria Antonio Narro, Saltillo, Mexico, during 2020-2021. Methodology: Lots of 50 grams dry weight of “Jaguar” habanero pepper seed samples were freeze dried and prepared with several organic solvents for the extraction, purification and identification of gibberellins and cytokinins using the techniques of GC-MS and GCMS-SIM respectively. Results: Gibberellins A1, A4, A7, A9, A15, A17 and A44 were found in habanero seed tissue. The cytokinins zeatin and zeatin-riboside were also detected in analyzed habanero samples.  Conclusion: The endogenous gibberellins A1, A4, A7, A9, A15, A17, A44, and the cytokinins zeatin and zeatin-riboside are present in habanero pepper cv “Jaguar” contributing to an improve seed viability, ensuring health and overall plant yield.

scholarly journals Effect Of Seed Hydration-Dehydration Integrated With Rhizobacteria On Viability And Vigor Of Deteriorated Soybean Seed

2018 ◽  
Vol 1 (1) ◽  
pp. 25-31
Author(s):  
P.K. Dewi Hayati
Keyword(s):  
Seed Viability ◽  
Soybean Seed ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Time Data ◽  
Randomized Design ◽  
Vigor Index ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Hydration And Dehydration

Soybeans undergo rapid deterioration due to its chemical composition and unfavourable storage conditions. The objective of the research was to determine the length period of seed hidration and dehydration which is integrated with rhizobateri to viability and vigor of deteriorated soybean seed.  A completely randomized design with four replicates were used in this experiment. Seeds were treated with varied combination of hydration period in a rhizobacteria suspension and followed by dehydration time.  Data were analysed using the F-test and significant differences were further tested with Least Significant Difference at the 5% level. Results showed that the hydration and dehydration period of seeds in a rhizobacteria suspension improved viability and vigor of seed which have 59.5% initial germination percentage. The improvement was 12.28%, 0.56, 25.4% and 1.4 days for standard germination test, vigor index, first count test percentage and T50, respectively. The hydration for 60 minutes followed by dehydration for 60 minutes gave the best results on seed viability and vigor.

scholarly journals PENGARUH UMUR PANEN RIMPANG TERHADAP PERUBAHAN FISIOLOGI DAN VIABILITAS BENIH JAHE PUTIH BESAR SELAMA PENYIMPANAN

2016 ◽  
Vol 21 (1) ◽  
pp. 17
Author(s):  
DEVI RUSMIN ◽  
M.R. SUHARTANTO ◽  
SATRIYAS ILYAS ◽  
DYAH MANOHARA ◽  
ENY WIDAJATI
Keyword(s):  
Weight Loss ◽  
Seed Viability ◽  
High Growth ◽  
Dry Weight ◽  
Zingiber Officinale ◽  
Shoot Height ◽  
Harvesting Time ◽  
Seed Technology ◽  
Randomized Design ◽  
Growth Ability

<p>ABSTRAK<br />Salah satu faktor yang menentukan daya simpan benih jahe putih<br />besar (JPB) adalah mutu. Mutu benih sangat ditentukan oleh tingkat<br />kemasakan rimpang. Penelitian bertujuan untuk mengetahui pengaruh<br />umur panen terhadap perubahan fisiologi dan viabilitas benih selama<br />penyimpanan. Penelitian dilaksanakan di Rumah Kaca dan Laboratorium<br />Teknologi Benih, Balai Penelitian Tanaman Rempah dan Obat serta<br />Laboratorium Pascapanen IPB Bogor, mulai bulan Juli 2012 sampai<br />dengan Februari 2013. Rancangan yang digunakan adalah acak lengkap<br />(RAL) dengan lima ulangan. Perlakuan yang diuji adalah tiga tingkat umur<br />panen benih 7, 8, dan 9 bulan setelah tanam (BST). Pengamatan dilakukan<br />terhadap perubahan fisiologis (penyusutan bobot, persentase rimpang<br />bertunas, tunas, kadar air, dan laju respirasi), serta viabilitas rimpang benih<br />(daya tumbuh, tinggi, dan bobot kering bibit). Hasil penelitian<br />menunjukkan rimpang benih umur 7 dan 8 BST mempunyai daya simpan<br />terbaik karena menghasilkan masing-masing total angka penyusutan bobot<br />lebih rendah (24,65 dan 25,25%) dan tunas lebih pendek (0,30 dan 1,08<br />cm) dibandingkan dengan umur panen 9 BST (27,13% dan 1,62 cm),<br />selama 4 bulan disimpan. Masa dormansi rimpang benih JPB mulai pecah<br />setelah mengalami periode simpan 2 bulan. Pertumbuhannya mulai<br />seragam setelah 3 bulan simpan. Umur panen jahe 7 dan 8 BST<br />mempunyai derajat dormansi yang lebih tinggi dibanding 9 BST. Rimpang<br />benih umur panen 7, 8, dan 9 BST mempunyai daya tumbuh tinggi (&gt;95%)<br />dan pertumbuhan bibit seragam setelah 3 bulan disimpan.<br />Kata kunci: Zingiber officinale Rosc., penyimpanan, benih, perubahan<br />fisiologis, viabilitas</p><p>ABSTRACT<br />One of the factors that determine the storability of seed rhizome of<br />white big ginger (WBG) is quality. The quality is determined by the<br />maturity levels of seed rhizome. The aim of the experiment was to observe<br />the effect of harvesting time on physiological changes and seed viability of<br />WBG seed rhizomes during the storage. The experiment was conducted at<br />Green House and Seed Technology Laboratory of Indonesian Spice and<br />Medicinal Crops Research Institute, Bogor and Postharvest Laboratory,<br />IPB, from July 2012 to February 2013. The experiment was arranged in a<br />completely randomized design with five replications. The treatments<br />tested were three levels of WBG seed rhizome harvesting time: 7, 8, and 9<br />month after planting (MAP). Variables observed were physiological<br />changes of seed rhizomes during the storage (weight loss, sprouting<br />percentage, shoot height, respiration rate, and moisture content) and<br />viability (growth ability, height, and dry weight of the seedling). The<br />results showed that seed rhizomes at 7 and 8 had the best storability, since<br />it was produced each low rate of weight loss (24.65 and 25.25%), and<br />shoots shorter (0.3 and 1.08 cm) than 9 MAP (27.13% and 1.62 cm), for 4<br />months in storage. Dormancy of WBG seed rhizomes has been broken<br />after 2 months in storage. Harvesting at 7 and 8 showed a degree of<br />dormancy higher than the harvesting age 9 MAP. Harvesting time at 7, 8,<br />and 9 MAP had high growth ability (&gt; 95%) and uniform seedling growth<br />after 3 months in storage.<br />Keywords: Zingiber officinale Rosc., storage, seed, physiological<br />changes, viability</p>

scholarly journals Salinity Tolerance of Mungbean Genotypes at Seedling Stage

2018 ◽  
Vol 10 (2) ◽  
pp. 409-416
Author(s):  
Ratri Tri Hapsari ◽  
Trustinah Trustinah
Keyword(s):  
Germination Rate ◽  
Lateral Roots ◽  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seedling Stage ◽  
Fresh Weight ◽  
Vigor Index ◽  
Normal Seedling ◽  
Two Factors

Salinity is a major abiotic stress limiting mungbean production worldwide including Indonesia. Since mungbean plant is very sensitive to salt condition, selection of salinity tolerant genotypes becomes important for mungbean improvement. The objective of this study was to evaluate the tolerance of eight mungbean genotypes to salinity at seedling stage under different levels. The experiment was arranged in a randomized complete block design with two factors (mungbean genotypes and salinity levels) and triplicates. Observation variables were germination percentage, vigor index, germination rate, hypocotyls length, epicotyls length, root length, number of root, seedling fresh weight, and seedling dry weight. The result showed that increasing level of salinity concentration inhibited the speed of germination, germination percentage, vigor index, normal seedling fresh weight, and number of lateral roots. Murai and Vima 1 were identified as tolerant genotypes, while Vima-2 and MLGV 0180 were identified as salinity sensitive genotypes at seedling stage. Currently, mungbean varieties with special characters, such as saline-tolerant is not yet available. The availability of saline-tolerant variety of mungbean is a cheaper and easier technology for farmers to anticipate the expansion of the saline area. The tolerant genotypes may be further tested at the later stage to obtain promising genotype tolerant to salinity that effectively assist mungbean breeding program.

scholarly journals The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage

Jurnal Wasian ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 133-143
Author(s):  
Iskandar Siregar ◽  
◽  
Riki Ramdhani ◽  
Evayusvita Rustam ◽  
Dede Sudrajat ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Seed Viability ◽  
Germination Capacity ◽  
Peg 6000 ◽  
Vigor Index ◽  
Fine Bubble ◽  
Initial Seed ◽  
Environmental Air

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

scholarly journals The Effect of Cocopeat and Charcoal Combination in Growing Media of Green Mustard (Brassica rapa L.) Var. Parachinensis Growth

2020 ◽  
Vol 7 (1) ◽  
pp. 62-66
Author(s):  
Masniar Vina Kesti ◽  
Bambang Irawan ◽  
Priyambodo Priyambodo ◽  
Martha Lulus Lande
Keyword(s):  
Dry Weight ◽  
Growing Media ◽  
Randomized Design ◽  
Vigor Index ◽  
Growing Medium ◽  
Significant Difference ◽  
Seedling Roots ◽  
Completely Randomized Design ◽  
Water Gas ◽  
Difference Test

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.

scholarly journals Pengaruh Dosis Minyak Cengkeh Terhadap Populasi Hama Callosobruchus chinensis L., Viabilitas Dan Vigor Benih Dua Kultivar Kacang Hijau Setelah Periode Simpan Tiga Bulan

Agrologia ◽  
2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Siti Syarah Nurbaekah ◽  
Sumadi Sumadi ◽  
Anne Nuraini
Keyword(s):  
Mung Bean ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Vigor ◽  
Callosobruchus Chinensis ◽  
Clove Oil ◽  
Bean Cultivar ◽  
Randomized Design ◽  
Vigor Index ◽  
Completely Randomized Design

Callosobruchus chinensis L. still become the primary pest of mung bean, it can cause damage until 70 percent. Pest control on mung bean seeds can be done by using anorganic protectant and bio protectant. The aim of the research was to determine the dosage of clove oil that could suppressed the population of weevil C. chinensis L. and maintaining seed viability and seed vigor of two mung bean cultivar after three months storage. The experimental design was Completely Randomized Design with 10 treatments combination of cultivar and clove oil dosage; all treatment were replicated three times. The mungbean seeds were cultivar Vima 1 and Vima 3.  The clove oil dosages used were 0 mL kg-1, 0,5 mL kg-1, 1,0 mL kg-1, 1,5 mL kg-1 and 2,0 mL kg-1. The mungbean result of experiment showed that there were significant effect between cultivar and clove oil dosage on germination rate percentage after three months storage, but no significant effect on weevil population and vigor index. The dosage of 1,5 mL kg-1 clove oil was the better treatment to maintaining seed viabilty andits vigor.Keywords: Mung bean, C. chinensis L., Clove oil, Seed viability and Seed Vigor.

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