scholarly journals Organic antioxidant dynamics in Hydrangea macrophylla Ser. leaves in humid subtropics of Russia

Author(s):  
V. I. Malyarovskaya ◽  
V. N. Bekhterev ◽  
O. G. Belous

Subtropical ornamental crops have been cultivated and studied in the humid subtropics of Russia for over a century. Nevertheless, a comprehensive evidence on their adaptation and stress is rather scarce for the region. The climatic conditions in Russian humid subtropics may occur extreme to introduced plants, which warrants research into their adaptive reactions to soil aridity and summer temperatures over +30 C°. Given the situation, understanding the mechanisms of main stressor-adaptive responses is relevant in ornamental crops, including Hydrangea macrophylla Ser. Gas chromatography-mass spectrometry  was  employed  to study ethanol leaf extracts in various-hardiness Hydrangea macrophylla Ser. cultivars, relatively hardy (Draps Wonder, Admiration, Altona), medium-hardy  (Souer Theresa) and susceptible (Harlequin, Madame Faustin) forms. Eleven endogenous organic compounds have been identified, of most interest being antioxidants and stress-protectants, including diarylethylene aromatic hydrocarbons (2,4’-dihydroxystilbene), squalene and phytosterines (y/p-sitosterol). The organic leaf content changed in response to hydrothermal stress in H. macrophylla, with a maximal concentration (2,4’-dihydroxystilbene 14.0, sitosterol 5.7 %) observed in favourable hydrothermal conditions of April, and minimal —  in the first August decade (8.5 and 1.7 %, respectively). Meanwhile, the relatively hardy Draps Wonder, Admiration and Altona varieties possessed the highest organic content of 2,4’-dihydroxystilbene (18.4,  21.5,  21.6 %) and y/p-sitosterol (5.7,  7.5,  6.0 %) both in optimal and stressing times. The lowest synthesis in the periods was observed in the unstable H. macrophylla varieties, Madame Faustin (6.9 and  1.1 %) and Harlequin (7.6 and  1.4 %).

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2949
Author(s):  
Juan I. Burneo ◽  
Ángel Benítez ◽  
James Calva ◽  
Pablo Velastegui ◽  
Vladimir Morocho

Chemical compositions of plants are affected by the initial nutrient contents in the soil and climatic conditions; thus, we analyzed for the first time the effects of soil and leaf nutrients on the compositions of the essential oils (EOs) of Siparuna muricata in four different localities in Ecuador. EOs were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) and a gas chromatography/flame ionization detector (GC/FID). Enantiomeric distribution by GC/MS was determined, modifying the enantiomeric separation of β-pinene, limonene, δ-elemene, β-bourbonene, cis-cadina-1 (6), 4-diene and atractylone. A total of 44 compounds were identified. The most representative for L1 were guaiol, atractylone and 4-diene; for L2, cis-cadina-1(6),4-diene and myrcene; for L3, atractylone, myrcene and germacrene B; and finally, L4 germacrene B, myrcene and cis-cadina-1(6),4-diene. Correlations between soil- leaf chemical elements such as Al, Ca, Fe, Mg, Mn, N and Si in the different localities were significant with chemical composition of the essential oil of Siparuna muricata; however, correlations between soil and leaf K, P, and Na were not significant. Cluster and NMDS analysis showed high dissimilarity values of secondary metabolites between four localities related with changes in soil- leaf nutrients. Thus, the SIMPER routine revealed that not all secondary metabolites contribute equally to establishing the differences in the four localities, and the largest contributions are due to differences in guaiol, cis-cadina-1(6),4-diene, atractylone and germacrene. Our investigation showed for the first time the influences of altitude and soil- leaf chemical elements in the chemical composition of the EOs of S. muricata.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1294
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelbaset M. Elgamal ◽  
Yasser A. EI-Amier ◽  
Tarik A. Mohamed ◽  
Abd El-Nasser G. El Gendy ◽  
...  

The integration of green natural chemical resources in agricultural, industrial, and pharmaceutical applications allures researchers and scientistic worldwide. Cleome amblyocarpa has been reported as an important medicinal plant. However, its essential oil (EO) has not been well studied; therefore, the present study aimed to characterize the chemical composition of the C. amblyocarpa, collected from Egypt, and assess the allelopathic, antioxidant, and anti-inflammatory activities of its EO. The EO of C. amblyocarpa was extracted by hydrodistillation and characterized via gas chromatography–mass spectrometry (GC-MS). The chemometric analysis of the EO composition of the present studied ecospecies and the other reported ecospecies was studied. The allelopathic activity of the EO was evaluated against the weed Dactyloctenium aegyptium. Additionally, antioxidant and anti-inflammatory activities were determined. Forty-eight compounds, with a prespondence of sesquiterpenes, were recorded. The major compounds were caryophyllene oxide (36.01%), hexahydrofarnesyl acetone (7.92%), alloaromadendrene epoxide (6.17%), myrtenyl acetate (5.73%), isoshyobunone (4.52%), shyobunol (4.19%), and trans-caryophyllene (3.45%). The chemometric analysis revealed inconsistency in the EO composition among various studied ecospecies, where it could be ascribed to the environmental and climatic conditions. The EO showed substantial allelopathic inhibitory activity against the germination, seedling root, and shoot growth of D. aegyptium, with IC50 values of 54.78, 57.10, and 74.07 mg L−1. Additionally, the EO showed strong antioxidant potentiality based on the IC50 values of 4.52 mg mL−1 compared to 2.11 mg mL−1 of the ascorbic acid as standard. Moreover, this oil showed significant anti-inflammation via the suppression of lipoxygenase (LOX) and cyclooxygenases (COX1, and COX2), along with membrane stabilization. Further study is recommended for analysis of the activity of pure authentic materials of the major compounds either singularly or in combination, as well as for evaluation of their mechanism(s) and modes of action as antioxidants or allelochemicals.


2021 ◽  
Vol 54 (2) ◽  
pp. 57-62
Author(s):  
Daria G. Fedorova ◽  
Natalia M. Nazarova ◽  
Yulia F. Kuhlevskaya

Abstract. The work was carried out to modify the method of assessing the viability of plants, taking into account the limiting factors of the climatic conditions of the Orenburg Preduralie. Are studied several species of plants, during the introduction at the steppe zone (on example of Оrenburg). Introduction study of all taxons was carried out for 8 years (20122020). The results of visual and laboratory observations of the seasonal development of species. During the entire observation period such indicators, as lignification of shoots, heat resistance, drought resistance, winter hardiness, shoot-forming capacity, height increase, generative development, and possible ways of reproduction in culture were evaluated annually. For each indicator numerical values in points corresponding to a certain state of the plant were selected. Based on the integrated assessment, the total viability score was calculated separately for each year of observations and the average score for the observation period. The sum of the average scores is an integral numerical expression of the viability of the introduced plants. Was established that the studied species belong to the I and II group relatively the criterion of life skills. The most species and sorts are among the most promising plants for introduction.


2019 ◽  
Vol 96 (8) ◽  
pp. 766-769 ◽  
Author(s):  
Inessa V. Averyanova ◽  
S. I. Vdovenko ◽  
A. L. Maksimov

Natural and climatic conditions of the environment of Northeast Russia and particularly Magadan region are the very factor mostly influencing adaptive responses by individuals inhabiting the region. Compensatory and adaptive responses in indigenes and newcomers of the region can be assumed to have their specific features. In 2009 there was executed the examination of the cardiovascular and respiratory systems and gas exchange in 392 cases aged of 17-19 years, including Europeans (Caucasians) born in the North in the 1st-2nd generation and indigenes. The methodologically similar study was carried out in 2014 in 265 persons, referred to the same cohorts of North-born Caucasians and Indigenes from the Magadan region. The results of the study executed in 2009 testified to a small number of physiological parameters that were reliably different in Caucasians vs. Indigene subjects. In 2014 no difference was found between the two examined cohorts throughout the observed parameters. The revealed changes in gas exchange, external respiration and cardiovascular systems demonstrated by modern young Indigenes of Northeast Russia testified to the fall in the effectiveness of their breathing. All that makes them farther from the classic “polar metabolic type” and their morphofunctional status becomes closer to European male subjects of Northeast Russia. Thus, we can observe a clear tendency towards “convergence in programs” of the adaptive changes between populations of the North residents undergoing similar natural, environmental and social factors.


2018 ◽  
Vol 8 (1) ◽  
Author(s):  
A.G.W.U. Perera ◽  
M.M.S.C. Karunaratne ◽  
S.D.M. Chinthaka

Neem (Azadirachta indica A. juss), is known to possess a wide range of pharmacological properties and is thus commercially exploitable. Apart from its medicinal potential, a considerable progress has been achieved regarding biological potential and chemical composition of the leaves which is an ever-increasing interest to the scientific community. During this study, biological phenomena and secondary metabolite composition of A. indica leaves were examined in the management of Sitophilus zeamais on stored maize. Insecticidal and repellent potential of A. indica leaf powders were evaluated in both contact and fumigant forms. Phytochemical screening of 11 phyto constituents was performed following the standard procedures for n-hexane, dichloromethane, ethyl acetate, methanol and aqueous leaf extracts. Volatile profile of A. indica leaves was characterized by employing headspace-solid-phase micro extraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS). Over 60% weevil repellency was recorded at doses above 23.33%, whereas 100% and 67% contact and fumigation mortalities were observed respectively, 9 days after treatment at the dose of 33.33% and the respected LD50 values were 1.56 g and 4.48 g. Thirty two volatile compounds were identified in three distinct chemical classes (Monoterpenoid, sesquiterpenoid and purine nucleosides). γ-Elemene (24.06%), 3,7 (11)-eudesmadiene (6.83%), caryophyllene (6.40%), and 10s,11s-himachala-3(12),4-diene (6.36%) were the major constituents of neem leaf volatiles, followed by other compounds present in less than 4% which might be responsible for varied biological activities observed. Thus the odour impact of the bioassayguided study clearly implies that A. indica leaves can be harnessed against S. zeamais infestations.Keywords: Azadirachta indica, Sitophilus zeamais, headspace-solid-phase micro-extraction, insecticidal activity, repellency


2020 ◽  
Vol 16 (2) ◽  
Author(s):  
P. M. Ridzuan ◽  
Hairul Aini Hamzah ◽  
Anis Shah ◽  
Norazian Mohd Hassan ◽  
Baharudin Roesnita

Antibacterial activity of different types of P. odorata leaf extracts was evaluated in combination with standard antibiotics. Persicaria. odorata leaves were extracted with n-hexane (n-hex), dichloromethane (DCM) and methanol (MeOH).  Each extract was applied on vancomycin (30µg), erythromycin (15µg) and gentamicin (10µg) discs, respectively. Disk diffusion method was used to evaluate the synergistic activity of each combination on Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, and Escherichia coli. Minimum inhibitory concentration (MIC) and gas chromatography mass spectrometry (GCMS) analysis was performed on the active extract. Synergistic effects seen were mainly from the n-hex+antibiotics combinations, mainly on the Gram-positive bacteria (7 additive, 5 antagonistic), with MIC range from 50 µg/ml to 100 µg/ml, as well as Gram-negative bacteria (2 additive, 2 indifferent, 5 antagonistic). In particular, synergism showed by the combination of n-hex+van were all additive against the susceptible bacteria. DCM extract combination showed synergistic effects on three Gram-positive species (S. aureus, S. epidermidis, S. pyogenes). Meanwhile, MeOH+antibiotics combination showed significant additive synergistic effects (p<0.05) on S. aureus and S. epidermidis.  The major compounds of leaves extract were decanal and β-citral. n-Hex extract superiorly inhibited Gram-positive bacteria growth as compared to DCM and MeOH extracts. The additive synergistic property of the n-hex P. odorata extract could be further studied for possible use as an antibacterial agent.


The Holocene ◽  
2019 ◽  
Vol 29 (11) ◽  
pp. 1731-1742 ◽  
Author(s):  
Ming Huang ◽  
Cheng Zhu ◽  
Chunmei Ma ◽  
Kunyu He ◽  
Jiajia Xu

The Baodun Culture (4600–3700 a BP) is currently the earliest archeological culture found at the Chengdu Plain in southwestern China. Archeological evidence has shown that the Baodun Culture likely originated from the upper reaches of the Minjiang River, with two possible routes for human migration into the lower Chengdu Plain proposed: (1) At about 5000 a BP, favorable climatic conditions in the upper reaches of the Minjiang stimulated the growth of populations and the development of agriculture in the region. Due to this shift, some groups expanded from the upper Minjiang River to sites in the northern area of the Chengdu Plain, such as the Guiyuanqiao site; (2) during 4800–4300 a BP, a drying event occurred in the upper regions of the river valley causing a reduction in agricultural harvests and the migration of populations into sites – including the Baodun site – from this area of the Minjiang to the southwest piedmont of the Chengdu Plain. A multi-facetted study of the T3321 profile at Baodun site, including pollens, grain size, and geochemistry, demonstrates that after 6000 a BP, the climate of the Chengdu Plain shifted from cool, toward warmer and dryer conditions. However, as the Chengdu Plain is located in an alluvial fan, hydrothermal conditions here are still generally better than those in the upper reaches of Minjiang River. A change in the proportion of Concentricystes seen in the profile T3321 also indicates that after 4700 a BP, the lake and marsh areas associated with the Baodun site further decreased. However, as a result of this reduction, more terrestrial surfaces were exposed, providing favored space for Baodun Cultural groups in the area of the site. Due to such conditions, by about 4600 cal. a BP, people in the upper reaches of the Minjiang River had primarily moved to the southwest piedmont regions of the Chengdu Plain.


2019 ◽  
Vol 32 (1) ◽  
pp. 36-40 ◽  
Author(s):  
Tran Thi Kim Ngan ◽  
Dinh Thi Thu Thuy ◽  
Tran Thi Tuyen ◽  
Cam Thi Inh ◽  
Hoang Thi Bich ◽  
...  

This study presents a chemometric study on agarwood (Aquilaria crassna) essential oils extracted from selected agarwood samples grown in various regions of Asia. Adopting gas chromatography-mass spectrometry (GC-MS) technique, it was revealed that essential oils, produced by hydrodistillation,constitutes mainly volatile aromatic compounds. Several major components are shared in all samples including dihydro-agarofuran-15-al, jinkoeremol, 10-epi-γ-eudesmol, agarospirol, valerianol, n-hexadecanoic acid, neopetasane and dihydrokaranone. Despite differences in composition, extraction yield and detected constituents found in analyzed samples, characteristic aromatic compounds were abundantly found in the Agarwood essential oil. These discrepancies could be due to cultivation season, climatic conditions and extraction methods. Unambiguous identification of components in agarwood essential oils thereby opens new potential in the application of high-value aromatic compounds in agarwood essential oil in cosmetic products, perfumes, and pharmaceuticals.


2006 ◽  
Vol 84 (4) ◽  
pp. 600-608 ◽  
Author(s):  
R.S. Prezant ◽  
E.J. Chapman ◽  
A. McDougall

Predator-induced defenses are well-known adaptive responses found in many marine and freshwater organisms. These responses usually occur in juveniles or actively growing adults and reduce the ability of a predator to consume its prey. We report a predator-induced response occurring in utero in the viviparid snail Bellamya chinensis (Gray, 1863) that results in significant changes in offspring number, shell size variability of released juveniles, and juvenile shell organic content. Water-borne cues derived from a predatory crayfish ( Orconectes limosus (Rafinesque, 1817)) induced a doubling of the number of offspring released; these newly born snails were generally smaller, though more variable in size, and possessed a greater shell organic content than controls. The generally smaller size of juveniles released in the presence of a predator reflects a faster rate of generation and passage through the uterus. These responses to the presence of a predator could be adaptive by decreasing predation pressure within a population or by reducing mortality following failed attacks.


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