scholarly journals Uji Bakteri Coliform dan (Escherichia coli) pada Air Tercemar Dengan Penggunaan Susunan Filter

2021 ◽  
Vol 7 (1) ◽  
pp. 26-32
Author(s):  
Linda Purwanti ◽  
Ahmad Syauqi ◽  
Saimul Laili
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
T Test ◽  
P Value ◽  
Well Water ◽  
Clean Water ◽  
Paired Samples ◽  
Normally Distributed

Clean water is a serious problem in Indonesia, the availability of clean water has shrunk drastically due to deteriorating environment and pollution. Drinking water that meets safety standards is increasingly scarce. More than 100 million people need safe water sources. Filter media is able to remove chemicals in water such as turbid, odor, oily, yellowish, rusty and muddy making it unfit for consumption. In addition, the purpose of the clarification results must also be considered whether to drink household needs or other needs. This study uses Lauryl Tryptose Broth (LTB) media and petrifilm media. Petrifilm results show that it is very easy to count the bacteria Colifrom and Escerichia coli using petrifilm media. From the analysis of calculations using the Paired Samples T-Test on the sample before filtering and after filtering it was obtained the Tcount value of 2.28 with a P value 0.015 which showed very successful or significant results between the samples before and after the filter. In this analysis have normally distributed data with a significant value of P = 0.015 (<0.05). In this case the Paired Samples T-Test was tested for normality (Shapiro-Wilk) and the results were obtained in the form of a P value of <.001 which would indicate that the data was normally distributed, indicated by a P value <0.05. The filter on this filter is very useful for filtering on Escerichia coli bacteria because this filter removes the bacteria, but Colifrom bacteria can still escape the use of the filter, because it depends on environmental conditions. Colifrom and Escerichia coli bacteria occur is seen from the relationship between sources of pollution such as, for example, such as septic tanks, distance of wells with waste, trash, and livestock pollution. The quality of drinking water is very close to the presence of Colifrom and Escerichia coli bacteria which are likely to have pathogenic bacteria that are harmful to health in the quality of well water. Water samples to be observed using well water using 2 treatments as many as 12 repetitions. Thus the total sample obtained as many as 24 water samples will be observed. While the test making of Lauryl Triptose Broth medium was observed by incubating 48 hours at 35oC + gas. Before making the test tube medium must be sterilized first. Subsequent tests were observed with a petrifilm medium to determine the presence or absence of Colifrom and Escherichia coli bacteria by looking at the color of the battery being sought. Keywords: Drinking Water, Coliform Bacteria and Escherichia coli    

scholarly journals Quality of Drinking Water in the Balkhash District of Kazakhstan’s Almaty Region

Water ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 392 ◽  
Author(s):  
Sabir Nurtazin ◽  
Steven Pueppke ◽  
Temirkhan Ospan ◽  
Azamat Mukhitdinov ◽  
Timur Elebessov
Keyword(s):  
Drinking Water ◽  
Surface Water ◽  
Water Samples ◽  
Well Water ◽  
Chemical Impurities ◽  
Tube Wells ◽  
Local Residents ◽  
High Concentrations ◽  
Growth Economic

The thinly populated Balkhash District of Kazakhstan’s Almaty Region lies in the lower reaches of the Ili-Balkhash basin, which is shared by China and Kazakhstan. The district is arid and heavily dependent on inflows of surface water, which are threatened by the effects of upstream population growth, economic development, and climate change. The quality of drinking water from centralized water systems and tube wells in nine villages of the district was analyzed, and the organoleptic properties of water from these sources was also assessed by an expert and via surveys of local residents. Although most samples met governmental standards for the absence of chemical impurities, high concentrations of mineralization, chlorides, boron, iron, and/or uranium were present in some well water samples. Levels of these pollutants were as much as 4-fold higher than governmental maxima and as much as 16-fold higher than concentrations reported previously in surface water. All centralized water samples met standards for absence of microbial contamination, but total microbial counts in some well water samples exceeded standards. Organoleptic standards were met by all the water from five villages, but centralized water from one village and well water from four villages failed to meet standards based on expert judgment. Residents were, for the most part, more satisfied with centralized rather than well water, but there was no obvious relationship between the failure of water to meet standards and the locations or populations of the settlements. This is the first comprehensive assessment of groundwater used for drinking in the lower Ili-Balkhash basin, and although it relies on a limited number of samples, it nevertheless provides evidence of potentially serious groundwater contamination in the Balkhash District. It is thus imperative that additional and more detailed studies be undertaken.

scholarly journals Comparison of four β-glucuronidase and β-galactosidase-based commercial culture methods used to detect Escherichia coli and total coliforms in water

2014 ◽  
Vol 13 (2) ◽  
pp. 340-352 ◽  
Author(s):  
Andrée F. Maheux ◽  
Vanessa Dion-Dupont ◽  
Sébastien Bouchard ◽  
Marc-Antoine Bisson ◽  
Michel G. Bergeron ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Ease Of Use ◽  
Well Water ◽  
Positive Signal ◽  
Culture Methods ◽  
E Coli

The MI agar, Colilert®, Chromocult coliform® agar, and DC with BCIG agar chromogenic culture-based methods used to assess microbiological quality of drinking water were compared in terms of their ubiquity, sensitivity, ease of use, growth of atypical colonies and affordability. For ubiquity, 129 total coliform (representing 76 species) and 19 Escherichia coli strains were tested. Then, 635 1-L well water samples were divided into 100 mL subsamples for testing by all four methods. Test results showed that 70.5, 52.7, 36.4, and 23.3% of the non-E. coli total coliform strains and 94.7, 94.7, 89.5, and 89.5% of the 19 E. coli strains yielded a positive signal with the four methods, respectively. They also yielded a total coliform positive signal for 66.5, 51.7, 64.9, and 55.0% and an E. coli positive signal for 16.1, 14.8, 17.3, and 13.4% of the 635 well water samples tested, respectively. Results showed that Colilert® is the most expensive method tested in terms of reactants, yet it is the easiest to use. Large numbers of atypical colonies were also often observed on Chromocult coliform® and DC with BCIG, thereby challenging the target microorganism count. Thus, the MI agar method seems to be the best option for the assessment of drinking water quality.

scholarly journals PENGOLAHAN AIR PAYAU MENGGUNAKAN TEKNOLOGI MEMBRAN SISTEM OSMOSA BALIK SEBAGAI ALTERNATIF PEMENUHAN KEBUTUHAN AIR MINUM MASYARAKAT KEPULAUAN SERIBU

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Wahyu Widayat
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Reverse Osmosis ◽  
Brackish Water ◽  
Well Water ◽  
Clean Water ◽  
Small Islands ◽  
The People ◽  
Quality Of Water

Kepulauan seribu (Seribu Archipelago) is one of region of north jakarta, the province of DKI Jakarta Raya which is populated by around 18.000 inhabitants. It consists of 111 small islands. Among them, there are about 11 (eleven) islands are stated as the residential:   P. Untung Jawa, P. Tidung Besar, P. Lancang Besar, P. Panggang, P. Pramuka, P. Kelapa I, P. Kelapa II, P. Harapan, P. Sebira. P. Payung dan P. Pari. P. Panggang and P. Kelapa I are the most populated.In relation to the clean water supply, specifically for drinking water/freshwater, mostly is got from the narrow well, rainfall and some water treatment installations that heve been owned by some islands. However, problem arises when the prolong dry season comes as the quality of water in the narrow well decreases significantly, the well water became brackish. In order to cope this problem, the avaibility of sufficient water treatment installations to process brackish water into freshwater is very important. The water treatment installation with reverse osmosis system is one of the most effective alternative in order to provide the freshwater for the people in the islands. The system has also been aplied sufficiently in several islands, such as P. Kelapa I, P. Tidung, P. Pramuka, P. Untung Jawa, P. Panggang dan P. Harapan Kata Kunci : Pengolahan Air payau, Osmosa balik, Air minum.

scholarly journals Identifikasi Bakteri Escherichia coli pada Air Minum Isi Ulang yang Diproduksi DAMIU di Kelurahan Lubuk Buaya Kota Padang

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Rani Afrisetiawati ◽  
Erly Erly ◽  
Endrinaldi Endrinaldi
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Drinking Water ◽  
Laboratory Test ◽  
Human Body ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Bacteriological Test ◽  
Identification Test

AbstrakAir merupakan  komponen penting bagi tubuh.  Menurut Permenkes No.492 Tahun 2010 tentang syarat mikrobiologi air minum adalah tidak ditemukannya bakteri Coliform dan Escherichia coli dalam 100 ml air. Tujuan penelitian ini adalah mengetahui kualitas air minum yang diproduksi depot air minum isi ulang di Kelurahan Lubuk Buaya. Penelitian bersifat deskriptif dengan melakukan pemeriksaan bakteriologi sampel air di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas dari Februari  sampai Oktober 2014. Sampel adalah air dari masing-masing depot air minum isi ulang di Kelurahan Lubuk Buaya yang sudah dimasukkan ke dalam galon. Penelitian ini terdiri dari empat tes, yaitu tes presumtif, konfirmatif, lengkap dan identifikasi. Hasil pemeriksaan laboratorium menunjukkan empat dari dua belas sampel (33,3%) mengandung bakteri Coliform dan satu dari empat sampel tersebut mengandung Escherichia coli. Pengawasan terhadap depot air minum isi ulang di Kelurahan Lubuk Buaya harus lebih ditingkatkan. Kader Kesehatan setempat diharapkan dapat memberikan penyuluhan kepada pemilik atau operator depot agar memperhatikan kebersihan depot serta peralatan yang digunakan.Kata kunci: air minum isi ulang, Coliform, Escherichia  coli AbstractWater is an essential component for the human body. According to Permenkes No. 492 Tahun 2010 about microbiological standards for drinking water is the absence of coliform and Escherichia coli in 100 ml of water. The objective of this study was to find the quality of drinking water produced by drinking water refill depots at Lubuk Buaya Padang based on microbiological standards of drinking water.The research was conducted using by bacteriological test on 12 water samples at the Laboratory of Microbiology, Facultyof Medicine, University of Andalas from February until Oktober 2014. This research consisted  four tests: the presumptive test, confirmative test, completetest and identification test. Based on the result of laboratory test, there were four samples (33,3%) from twelve samples contain Coliform bacteria and one of them contain Escherichia coli. Controlling of drinking water refill depots at Lubuk Buaya should have been improved. The officers of Public Health at Lubuk Buaya must give an advice to the owners or depot operators about hygiene of depot and tools that they used.Keywords: drinking water refill, Coliform, Escherichia coli

Penetapan Kadar Klorida pada Air Sumur di STIKes Guna Bangsa Yogyakarta Tahun 2013

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Dian Wuri Astuti ◽  
Siti Fatimah ◽  
Rausyanah Fikriyyah
Keyword(s):  
Drinking Water ◽  
Chloride Concentration ◽  
Chloride Content ◽  
Water Source ◽  
Chloride Ions ◽  
Well Water ◽  
Clean Water ◽  
Drinking Water Contamination ◽  
Metal Pipes

Background : STIKes Guna Bangsa Yogyakarta was a health college which used well water as source of clean water for many needs, one of them use as drinking water. Contamination the source of water in STIKes Guna BangsaYogyakarta can occur cause activity of waste disposal directly into the land so that can give effect to quality of the water source well. The good water must be having four criteria or parameter. The first is physic parameter involve dissolved solid, turbidity, smell, taste, colour and temperature. Second parameter, biology parameter was involved type and contents of microorganism. Third parameter is radioactive was involved radioactive substance. The fourth there’s chemical parameter, it was involved many types of ion, dissolve oxygen or toxic compounds likes chloride. Chloride ions in the form of inorganic anion Cl- is a lot there in the water. The presence of excessive chloride in water can cause interference with the physical properties of water, disruption of metal pipes, and health problems. Lack of chloride element in body will be bring about the fall in value of the extracellular fluids osmotic and if excess, will be damage the kidney. Therefore, the writer interested to know of chloride rate in well water and choose title “Penetapan Kadar Klorida pada Air Sumur di STIKes Guna Bangsa Yogyakarta”. Examination of chloride in well water conducted to determine howlarge concentration of chloride content in well water, drinking water requirements are allowed in PerMenKes RI No. 492/MenKes/Per/IV/2010. Method : Chloride concentration in well water determined by Argentometry Mohr with standard solution of AgNO3 and K2CrO4 indicator, the titration end point is marked with red brick precipitation of Ag2CrO4. This research was descriptive research laboratory test. The sample in study was well water in Guna Bangsa Yogyakarta’s STIKes. Data analysis result of research done descriptively in table or graphic form. Result : Chloride concentration in well water of Guna Bangsa Yogyakarta’s STIKes with sample as many as 9 sample showed that accordance with PerMenKes RI 2010 were not exceed the maximum limit was 250 mg/L. Conclution : The sample of well water in Guna Bangsa Yogyakarta’s STIKes had chloride concentration were according with allow quality of clean water in accordance with PerMenKes RI No. 492/ Menkes/ Per/ IV/ 2010.

scholarly journals Quality Assessment of Drinking Well Water near Dumpsite at Ogidi-Oloje Ilorin, Kwara State, Nigeria

2020 ◽  
Vol 24 (2) ◽  
pp. 323-328
Author(s):  
K.O. Yusuf ◽  
I.B. Sadiku ◽  
O.S. Abioye
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Physicochemical Properties ◽  
Water Samples ◽  
Rainy Season ◽  
Dry Season ◽  
Well Water ◽  
Cadmium Lead ◽  
And Control

This study was conducted to determine the quality of drinking well water near dumpsite at Ogidi-Oloje, Ilorin, Kwara State. Open wells near dumpsite could be polluted due to percolation of chemicals from the decomposition of refuse and scraps of electronic boards having potential of causing diseases to man. Most people at Ogidi-Oloje Ilorin depend on open wells near dumpsite for drinking water during dry season. Water samples were collected from seven open wells (W1 - W6) 9.2 - 18.2 m away from dumpsite and the control well W7 was 45.0 m away from the dumpsite. Physicochemical and bacteriological properties were determined from the water samples during dry season (March, 2019) and rainy season (July, 2019). Phosphate, Cadmium, Lead, Feacal coliform were not detected during dry season but only Feacal coliform was not detected in the water during rainy season. All the values of physicochemical properties of the water were within WHO permissible limits except Iron and Cadmium that above the limits which could cause diseases to mankind. Values of Iron during dry season for six well sources were 3.61, 4.02, 4.26, 3.25, 5.14 and 7.24 mg/L while values for rainy season and control were 0.715, 1.410, 0.650, 0.049, 0.780, 1.110 and 0.775 mg/L(control) but WHO limits is 0.03mg/L. Concentrations of Cadmium in the water during rainy season were 0.010, 0.015, 0.015, 0.020, 0.015, 0.015 and 0.02mg/L(control) but WHO limit is 0.003 mg/L. This study revealed that the water is suitable for consumption during dry season but unsuitable during rainy season. Keywords: well water quality, groundwater pollution, water diseases, dumpsite

scholarly journals Comparison of the Amount of Escherichia Coli in Refilled Drinking Water at the Depot with Bottled Drinking Water

2021 ◽  
Vol 11 (5) ◽  
pp. 401-409
Author(s):  
Frisca Ronauli Batubara ◽  
Dame Joyce Pohan ◽  
Dora Elysia Octavia Pasaribu
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Positive Sample ◽  
Bottled Water ◽  
Clean Water ◽  
Pseudomonas Sp ◽  
E Coli ◽  
Bottled Drinking Water ◽  
Agar Media

Water needed for daily use is viable water that is clean and healthy. Due to limited access to clean water, people buy bottled water and refillable drinking water. It is necessary to do clinical research at the laboratory to determine whether the water condition is feasible or not for consumption. The purpose of this study was to determine whether or not there is E.coli bacteria in the refillable drinking water and bottled water. This study uses an experimental method. Samples were taken from 15 refillable drinking water depots in Kecamatan Rawalumbu Bekasi and 15 bottled water samples. The bacterias identification was made by planting the samples in Blood Agar media and EMB. The results showed four positive samples of E. coli bacteria (26.7%) in the refillable drinking water depot at Kecamatan Rawalumbu Bekasi and one positive sample of E. coli bacteria (6.7%) in bottled water. It was also found that other types of bacteria such as Coliform (6.7%), Pseudomonas sp. (40%), Klebsiella sp. (13.3%), Staphylococcus sp. (6.7%), and Streptobacillus Gram + (13.4%). Key words: Refillable Drinking Water, Bottled Water, Escherichia coli.

scholarly journals EFFECTIVENESS OF "SM" TOOL TO REDUCE DETERGENT LEVELS IN LAUNDRY WASTEWATER

2020 ◽  
Vol 39 (4) ◽  
pp. 199-202
Author(s):  
Tri Marthy Mulyasari
Keyword(s):  
Liquid Waste ◽  
T Test ◽  
P Value ◽  
Clean Water ◽  
Significant Difference ◽  
Before And After ◽  
Health Quality ◽  
The Impact ◽  
Paired T Test

Introduction: The use of detergent every year is increasing in line with the population growth rate in Indonesia. The impact of excessive use of detergent will result in pollution and damage to ecosystems and aquatic biota. The nature of detergents is to damage microorganisms. The dominant laundry waste comes from softeners and detergents. The research aimed to analyze the effectiveness of the "SM" tool to reduce detergent levels in laundry wastewater. Methods: This type of research was pre-experimental with a one-shot case study design. The independent variable was a tool to recycle laundry liquid waste into clean water "SM." The dependent variable was the detergent level (MBAS). The sample came from the laundry in the Kecamatan Baturraden area. Replication was carried out six times. Analyzing differences in detergent levels before and after being treated with the "SM" tool. The analysis used a paired T test. Result and discussion: The experimental results showed the “SM” tool could treat 960 liters of wastewater/day. The physical quality of the water after being treated using the “SM” tool, the water was colorless or clear, but it still smelled slightly fragrantly. The average level of detergent level checks before processing was 2.52 mg/L and after processing was 0.01 mg/L. The effective detergent parameter (MBAS) decreased by 99.51%, categorized as quite effective. The analysis using the paired T Test showed the p value of 0.239 with the lower and upper values being at one pole, meaning there was a significant difference between detergent levels before and after being processed utilizing the "SM" tool. Conclusion: Detergent levels (MBAS) after processing met the chemical quality requirements of clean water in accordance with Regulation of the Minister of Health Number 32 of 2017 concerning Environmental Health Quality Standards and Water Health Requirements for Sanitation Hygiene, Solus Per Aqua and Public Bathing Needs.

Analisis Kualitas Mikrobiologi Air Minum Isi Ulang di Depo Air Minum Isi Ulang Kelurahan Jemur Wonosari Kota Surabaya

2021 ◽  
Vol 7 (1) ◽  
pp. 159-166
Author(s):  
Ajeng Dwi Rahmawati ◽  
Akas Yekti Pulih Asih ◽  
Edza Aria Wikurendra
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Laboratory Tests ◽  
Laboratory Data ◽  
Microbiological Quality ◽  
Microbiological Analysis ◽  
Cross Sectional ◽  
Drinking Water Samples

Background: Based on the preliminary test using laboratory tests from three refill drinking water samples in Jemur Wonosari Village, all three were positive for Coliform. Therefore, researchers are interested in researching Analysis of the Microbiological Quality of Refilled Drinking Water at the Refill Drinking Water Depot in Jemur Wonosari Village. Objectives: Knowing the microbiological quality of refill drinking water produced at the refill drinking water depot in Jemur Wonosari Village. Research Methods: This research is a descriptive study with a cross-sectional quantitative approach using primary and secondary data sources. The population in this study were all 13 refill drinking water depots. The research takes the total population. Refillable drinking water samples were tested at the Gayung Sari Regional Health Service Laboratory. Data analysis used descriptive analysis by comparing the results of laboratory tests with Permenkes 492 of 2010. Results: The results showed that 13 samples found that 13 samples were positive for Coliform, and 13 samples were negative for Escherichia coli. According to Permenkes No. 429 of 2010, the maximum limit per 100 ml of sample is 0. So the 13 samples of refill drinking water based on microbiological parameters, namely Escherichia coli in refilled drinking water, meet the standard while the Coliform does not meet the standard. Conclusion: All refill drinking water depots in Keluraham Jemur Wonosari produce refilled drinking water whose quality does not meet the government's microbiological standards. There is a need for increased supervision and guidance at refill drinking water depots.   Keywords: Drinking Water, Microbiological Analysis, Refill Drinking Water Depot

scholarly journals DETEKSI Escherichia coli DAN Angka Paling Mungkin PADA AIR SUMUR DEKAT JAMBAN DIDAERAH WONOAYU, SIDOARJO

2020 ◽  
Vol 4 (1) ◽  
pp. 16
Author(s):  
Putri Anggraeni ◽  
Evy Ratnasari Ekawati
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Sea Water ◽  
Coliform Bacteria ◽  
Well Water ◽  
Pathogenic Microorganisms ◽  
Descriptive Research ◽  
Water Wells ◽  
Water Well

Water sources are grouped into several groups which are: sea water, surface water, and groundwater. According to the science of health, each person requires drinking water as much as 2.5 to 3 liters per day including the water in food. This research is descriptive research to find out the quality of the water wells in the area Wonoayu Sidoarjo. To find out if there are any impurities bacteria Escherichiacoliin well water. To find out the numbers of the bacteria Escherichia coliand APM on a water well. The results of the sudy of APM well water near the toilet in Wonoayu, Sidoarjo water quality is not recommended bacteriologically in this area. Whereas maximum levels of Escherichiacolipermitted for drinking water according to is 0 or is free from pathogenic microorganisms that originated in feces, while 50% of water samples in the area of  Wonoayu contains coliform. Coliform bacteria in great numbers along with the feces will pollute the environment with 5 positive samples results contain Escherichia coliand 5 negative samples of Escherichia coli.

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