Analytical Characterization and Inhibitor Detection in Liquid Phases Obtained After Steam Refining of Corn Stover and Maize Silage

The utilization of agricultural products and residues for the production of value-added and biobased products is a highly relevant topic in present research. Due to the natural recalcitrance of lignocellulosic biomass against enzymatic degradation, pretreatments are important requirement for further processes. For the raw material in this study, corn stover (CS) as highly available agricultural residue and maize silage (MS) as model substrate for an ensiled agricultural product were pretreated by steam refining. However, after processing a liquid fraction and fibers are present. Subsequent to steaming the fiber fraction is well characterized. Nonetheless, in depth characterizations of the filtrates are also important for their subsequent utilization. Decreasing molar masses from 7,900 g/mol to 1,100 g/mol for CS filtrates and 100.000–12.900 g/mol for MS filtrates were determined with increasing severity. Due to their proven inhibitory effect on microorganisms weak acids, furans and phenolic compounds within the liquid phased were analyzed. Especially formic acid increases with increasing severity from 0.27 to 1.20% based on raw material for CS and from 0.07 to 0.23% based on raw material for MS. Further GC/MS measurements indicate, that up to 8.25% (CS filtrate) and 5.23% (MS filtrates) of the total peak area is related to inhibitory phenols. Considering the data, detoxification strategies are of non-negligible importance for filtrates after steam refining and should be considered for further research and process or parameter optimizations. An alternative may be the application of milder process conditions in order to prevent the formation of inhibitory degradation products or the dilution of the gained filtrates.

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Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.963 ◽

2011 ◽

Vol 343-344 ◽

pp. 963-967 ◽

Cited By ~ 1

Author(s):

Zhang Qiang ◽

Anne Belinda Thomsen

Keyword(s):

Ethanol Production ◽

Corn Stover ◽

Simultaneous Saccharification And Fermentation ◽

Ethanol Concentration ◽

Ethanol Yield ◽

Liquid Fraction ◽

Raw Material ◽

Wet Oxidation ◽

Inhibition Effect ◽

Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous sacchariﬁcation and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

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Production of Carbohydrates from Cardoon Pre-Treated by Acid-Catalyzed Steam Explosion and Enzymatic Hydrolysis

Energies ◽

10.3390/en12224288 ◽

2019 ◽

Vol 12 (22) ◽

pp. 4288 ◽

Cited By ~ 5

Author(s):

Alessandro Bertini ◽

Mattia Gelosia ◽

Gianluca Cavalaglio ◽

Marco Barbanera ◽

Tommaso Giannoni ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Lignocellulosic Biomass ◽

Steam Explosion ◽

Degradation Products ◽

Liquid Fraction ◽

Raw Material ◽

Total Carbohydrate ◽

Carbohydrate Degradation ◽

Acid Catalyzed ◽

Pre Treatment

Cardoon (Cynara cardunculus) is a promising crop from which to obtain oilseeds and lignocellulosic biomass. Acid-catalyzed steam explosion is a thermochemical process that can efficiently pre-treat lignocellulosic biomass. The drawback is the production of a high number of carbohydrate degradation products in the liquid fraction that could inhibit microbial growth. In this work, the lignocellulosic biomass of cardoon, gathered from a dedicated field, were used as the raw material for the production of fermentable monosaccharides by employing acid-catalyzed steam explosion. The raw material was pre-soaked with a dilute 1% (w/w) sulfuric acid solution and then subjected to steam explosion under three different severity conditions. The recovered slurry was separated into solid and liquid fractions, which were individually characterized to determine total carbohydrate and inhibitor concentrations. The slurry and the washed solid fraction underwent enzymatic hydrolysis to release glucose and pentose monosaccharides. By conducting the pre-treatment at 175 °C for 35 min and hydrolyzing the obtained slurry, a yield of 33.17 g of monosaccharides/100 g of cardoon was achieved. At the same conditions, 4.39 g of inhibitors/100 g of cardoon were produced.

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Synthesis of CuO for Microwave-Assisted Pyrolysis of Biomass

Applied Sciences ◽

10.3390/app9245525 ◽

2019 ◽

Vol 9 (24) ◽

pp. 5525 ◽

Cited By ~ 1

Author(s):

Eri J. Paz-García ◽

Silvia P. Paredes-Carrera ◽

Sergio O. Flores-Valle ◽

Isis S. Rodríguez-Clavel ◽

Jesús C. Sánchez-Ochoa ◽

...

Keyword(s):

Particle Size ◽

Liquid Fraction ◽

Synthesis Method ◽

Reaction Times ◽

Value Added ◽

Raw Material ◽

Microwave Assisted ◽

Bio Oil ◽

Chemical Products ◽

Pyrolysis Of Biomass

In this study, CuO was synthesized as a microwave absorber in the pyrolysis of a biomass model (sugarcane bagasse). CuO was synthesized for 5 min of irradiation using the following techniques: microwave (MW), ultrasound (US), combined mode (MW-US), and conduction heating (CH) as a reference material. The use of these treatments promotes changes in the morphology, as MW and US generate leaves and monolithic faceted morphologies, respectively. Changes were also generated in some textural characteristics such as crystal size, surface area, and volume-pore size. They were produced as a consequence of changes in the conditions during the crystallization stage produced by the different irradiation types. The microwave-assisted pyrolysis was performed aiming for the maximum liquid fraction (bio-oil) in the products. The reaction time, the size of the biomass, and the CuO synthesis method were also analyzed. The following particle size (ps) intervals were studied: ps < 0.5 mm, 0.5 mm < ps < 1.7 mm, 1.7 mm < ps < 3.5 mm. The best conditions at 1160 Watts in the microwave were: 4 min of reaction, particle size lower than 0.5 mm, and CuO synthesized by US. The use of CuO in the pyrolysis almost triples the amount of the obtained liquid fraction, when compared with the pyrolysis without the use of a microwave absorbent. The CuO was reduced to Cu2O and Cu after the pyrolysis. In this work, a reduction in the reaction times from hours to minutes was achieved during the synthesis of CuO and the pyrolysis biomass. The liquid fraction (bio-oil) can be raw material to obtain value-added chemical products or biofuels.

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β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization

Microorganisms ◽

10.3390/microorganisms9010055 ◽

2020 ◽

Vol 9 (1) ◽

pp. 55

Author(s):

María Florencia Eberhardt ◽

José Matías Irazoqui ◽

Ariel Fernando Amadio

Keyword(s):

Bacterial Species ◽

Value Added ◽

Raw Material ◽

Activity Levels ◽

Treatment Technology ◽

Catalytic Domains ◽

Stabilization Ponds ◽

Cazy Database ◽

Genes Encoding ◽

Value Added Products

Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume produced and the high biological and chemical oxygen demands. Due to its composition, mainly lactose and proteins, it can be considered as a raw material for value-added products, through physicochemical or enzymatic treatments. β-Galactosidases (EC 3.2.1.23) are lactose modifying enzymes that can transform lactose in free monomers, glucose and galactose, or galactooligosacharides. Here, the identification of novel genes encoding β-galactosidases, identified via whole-genome shotgun sequencing of the metagenome of dairy industries stabilization ponds is reported. The genes were selected based on the conservation of catalytic domains, comparing against the CAZy database, and focusing on families with β-galactosidases activity (GH1, GH2 and GH42). A total of 394 candidate genes were found, all belonging to bacterial species. From these candidates, 12 were selected to be cloned and expressed. A total of six enzymes were expressed, and five cleaved efficiently ortho-nitrophenyl-β-galactoside and lactose. The activity levels of one of these novel β-galactosidase was higher than other enzymes reported from functional metagenomics screening and higher than the only enzyme reported from sequence-based metagenomics. A group of novel mesophilic β-galactosidases from diary stabilization ponds’ metagenomes was successfully identified, cloned and expressed. These novel enzymes provide alternatives for the production of value-added products from dairy industries’ by-products.

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Utilization of Cumbeba (Tacinga inamoena) Residue: Drying Kinetics and Effect of Process Conditions on Antioxidant Bioactive Compounds

Foods ◽

10.3390/foods10040788 ◽

2021 ◽

Vol 10 (4) ◽

pp. 788

Author(s):

João Paulo de Lima Ferreira ◽

Alexandre José de Melo Queiroz ◽

Rossana Maria Feitosa de Figueirêdo ◽

Wilton Pereira da Silva ◽

Josivanda Palmeira Gomes ◽

...

Keyword(s):

Phenolic Compounds ◽

Bioactive Compounds ◽

Fixed Bed ◽

Drying Kinetics ◽

Raw Material ◽

Process Conditions ◽

Temperature Range ◽

Water Diffusivity ◽

Pharmaceutical Industries ◽

Effective Water

The residue generated from the processing of Tacinga inamoena (cumbeba) fruit pulp represents a large amount of material that is discarded without proper application. Despite that, it is a raw material that is source of ascorbic acid, carotenoids and phenolic compounds, which are valued in nutraceutical diets for allegedly combating free radicals generated in metabolism. This research paper presents a study focused on the mathematical modeling of drying kinetics and the effect of the process on the level of bioactive of cumbeba residue. The experiments of cumbeba residue drying (untreated or whole residue (WR), crushed residue (CR) and residue in the form of foam (FR)) were carried out in a fixed-bed dryer at four air temperatures (50, 60, 70 and 80 °C). Effective water diffusivity (Deff) was determined by the inverse method and its dependence on temperature was described by an Arrhenius-type equation. It was observed that, regardless of the type of pretreatment, the increase in air temperature resulted in higher rate of water removal. The Midilli model showed better simulation of cumbeba residue drying kinetics than the other models tested within the experimental temperature range studied. Effective water diffusivity (Deff) ranged from 6.4890 to 11.1900 × 10−6 m2/s, 2.9285 to 12.754 × 10−9 m2/s and 1.5393 × 10−8 to 12.4270 × 10−6 m2/s with activation energy of 22.3078, 46.7115 and 58.0736 kJ/mol within the temperature range of 50–80 °C obtained for the whole cumbeba, crushed cumbeba and cumbeba residue in the form of foam, respectively. In relation to bioactive compounds, it was observed that for a fixed temperature the whole residue had higher retention of bioactive compounds, especially phenolic compounds, whereas the crushed residue and the residue in the form of foam had intermediate and lower levels, respectively. This study provides evidence that cumbeba residue in its whole form can be used for the recovery of natural antioxidant bioactive compounds, mainly phenolic compounds, with the possibility of application in the food and pharmaceutical industries.

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Optimization of a pretreatment and hydrolysis process for the efficient recovery of recycled sugars and unknown compounds from agricultural sweet sorghum bagasse stem pith solid waste

PeerJ ◽

10.7717/peerj.6186 ◽

2019 ◽

Vol 6 ◽

pp. e6186 ◽

Cited By ~ 2

Author(s):

Ting-Ting Jiang ◽

Yan Liang ◽

Xiang Zhou ◽

Zi-Wei Shi ◽

Zhi-Jun Xin

Keyword(s):

Solid Waste ◽

Sweet Sorghum ◽

Agricultural Waste ◽

Value Added ◽

Raw Material ◽

Alkali Concentration ◽

Sweet Sorghum Bagasse ◽

Amorphous Cellulose ◽

Sorghum Bagasse ◽

Value Added Products

Background Sweet sorghum bagasse (SSB), comprising both a dermal layer and pith, is a solid waste generated by agricultural activities. Open burning was previously used to treat agricultural solid waste but is harmful to the environment and human health. Recent reports showed that certain techniques can convert this agricultural waste into valuable products. While SSB has been considered an attractive raw material for sugar extraction and the production of value-added products, the pith root in the SSB can be difficult to process. Therefore, it is necessary to pretreat bagasse before conventional hydrolysis. Methods A thorough analysis and comparison of various pretreatment methods were conducted based on physicochemical and microscopic approaches. The responses of agricultural SSB stem pith with different particle sizes to pretreatment temperature, acid and alkali concentration and enzyme dosage were investigated to determine the optimal pretreatment. The integrated methods are beneficial to the utilization of carbohydrate-based and unknown compounds in agricultural solid waste. Results Acid (1.5−4.5%, v/v) and alkali (5−8%, w/v) reagents were used to collect cellulose from different meshes of pith at 25–100 °C. The results showed that the use of 100 mesh pith soaked in 8% (w/v) NaOH solution at 100 °C resulted in 32.47% ± 0.01% solid recovery. Follow-up fermentation with 3% (v/v) acid and 6.5% (w/v) alkali at 50 °C for enzymolysis was performed with the optimal enzyme ratio. An analysis of the surface topography and porosity before and after pretreatment showed that both the pore size of the pith and the amount of exposed cellulose increased as the mesh size increased. Interestingly, various compounds, including 42 compounds previously known to be present and 13 compounds not previously known to be present, were detected in the pretreatment liquid, while 10 types of monosaccharides, including D-glucose, D-xylose and D-arabinose, were found in the enzymatic solution. The total monosaccharide content of the pith was 149.48 ± 0.3 mg/g dry matter. Discussion An integrated technique for obtaining value-added products from sweet sorghum pith is presented in this work. Based on this technique, lignin and hemicellulose were effectively broken down, amorphous cellulose was obtained and all sugars in the sweet sorghum pith were hydrolysed into monosaccharides. A total of 42 compounds previously found in these materials, including alcohol, ester, acid, alkene, aldehyde ketone, alkene, phenolic and benzene ring compounds, were detected in the pretreatment pith. In addition, several compounds that had not been previously observed in these materials were found in the pretreatment solution. These findings will improve the transformation of lignocellulosic biomass into sugar to create a high-value-added coproduct during the integrated process and to maximize the potential utilization of agricultural waste in current biorefinery processing.

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Valorization of secondary feedstocks from the agroindustry by selective catalytic oxidation to formic and acetic acid using the OxFA process

Biomass Conversion and Biorefinery ◽

10.1007/s13399-021-01854-7 ◽

2021 ◽

Author(s):

Sebastian Ponce ◽

Stefanie Wesinger ◽

Daniela Ona ◽

Daniela Almeida Streitwieser ◽

Jakob Albert

Keyword(s):

Acetic Acid ◽

Formic Acid ◽

Reaction System ◽

Value Added ◽

Industrial Wastes ◽

Raw Material ◽

Gaseous Oxygen ◽

Water As Solvent ◽

Selective Catalytic Oxidation ◽

Major Interest

AbstractThe selective oxidative conversion of seven representative fully characterized biomasses recovered as secondary feedstocks from the agroindustry is reported. The reaction system, known as the “OxFA process,” involves a homogeneous polyoxometalate catalyst (H8PV5Mo7O40), gaseous oxygen, p-toluene sulfonic acid, and water as solvent. It took place at 20 bar and 90 °C and transformed agro-industrial wastes, such as coffee husks, cocoa husks, palm rachis, fiber and nuts, sugarcane bagasse, and rice husks into biogenic formic acid, acetic acid, and CO2 as sole products. Even though all samples were transformed; remarkably, the reaction obtains up to 64, and 55% combined yield of formic and acetic acid for coffee and cocoa husks as raw material within 24 h, respectively. In addition to the role of the catalysts and additive for promoting the reaction, the influence of biomass components (hemicellulose, cellulose and lignin) into biogenic formic acid formation has been also demonstrated. Thus, these results are of major interest for the application of novel oxidation techniques under real recovered biomass for producing value-added products. Graphical abstract

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Current Trends in the International Economic Activity of Ukraine

Central Ukrainian Scientific Bulletin. Economic Sciences ◽

10.32515/2663-1636.2021.6(39).47-55 ◽

2021 ◽

pp. 47-55

Author(s):

Ljudmila Romaniuk ◽

Keyword(s):

International Economic ◽

Economic Performance ◽

Economic Activity ◽

Public Diplomacy ◽

Technological Development ◽

Value Added ◽

Machine Building ◽

Raw Material ◽

High Tech ◽

Economic Relations

International economic activity occupies a special place in a complex system of the global economic relations. It reflects the mutual economic dependence of trade and economic activities of different countries of the world. The significance of the development of international economic performance of each country is growing. The purpose of the article is to determine the current state and trends of international economic performance of Ukraine, taking into account changes in the external environment. SWOT-analysis was introduced to identify strengths, weaknesses, opportunities, threats and ways to overcome weaknesses, solve problems in international economic activity, use strengths and opportunities. International economic activity is a powerful factor in the development of the country's economy and has a significant potential in regard of natural, economic and human resources, but the study has also revealed problems and negative trends. To identify trends in the effectiveness of the country's international economic activity, export-import performance indicators for 2019, 2020 and similar indicators for 2013 were analyzed. In 2020 a decline in exports in the machine-building industry is observed. Furthermore, the exports are dominated by the raw material component. The growth rate of exported goods refers to industries with a small share of value added. At the same time, imports are dominated by high-tech products, indicating a lack of strategy of technical and technological development, which leads to the deindustrialization of the country, which is a significant threat to the economy of Ukraine as a whole and its international economic activity. In the context of economic globalization, the importance of international economic relations is growing. To increase the efficiency of international economic performance it is crucial to address a number of domestic issues: stabilization of political situation, termination of military actions in the east of the country, overcoming corruption, ensuring technical and technological development, implementation of innovations at enterprises, development and implementation of multi-vector strategy, implementation of the strategy of public diplomacy in order to build a positive image of Ukraine. Further research will focus on assessing the effectiveness of international economic activity, identifying threats to national competitiveness and elaborating recommendations for overcoming them.

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Whey Utilization: Sustainable Uses and Environmental Approach

Food Technology and Biotechnology ◽

10.17113/10.17113/ftb.59.02.21.6968 ◽

2021 ◽

Vol 59 (2) ◽

Author(s):

Elizabeta Zandona ◽

Marijana Blažić ◽

Anet Režek Jambrak

Keyword(s):

Whey Proteins ◽

Organic Matter Content ◽

Value Added ◽

Edible Films ◽

Matter Content ◽

Raw Material ◽

Sustainable Utilization ◽

The Past ◽

Food Applications ◽

Value Added Products

The dairy industry produces large amounts of whey as a by- product or co-product, which has led to considerable environmental problems due to its high organic matter content. Over the past decades, possibilities of more environmentally and economically efficient whey utilisation have been studied, primarily to convert unwanted end products into a valuable raw material. Sustainable whey management is mostly oriented to biotechnological and food applications for the development of value-added products such as whey powders, whey proteins, functional food and beverages, edible films and coatings, lactic acid and other biochemicals, bioplastic, biofuels and similar valuable bioproducts. This paper provides an overview of the sustainable utilization of whey and its constituents, considering new refining approaches and integrated processes to covert whey, or lactose and whey proteins to high value-added whey-based products.

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Border Trade between Regions of Russia and Provinces of China

Vestnik NSU Series History and Philology ◽

10.25205/1818-7919-2021-20-10-103-116 ◽

2021 ◽

Vol 20 (10) ◽

pp. 103-116

Author(s):

Natalya S. Epifanova ◽

Vladimir N. Akulinin

Keyword(s):

Manufacturing Industry ◽

Raw Materials ◽

Value Added ◽

Raw Material ◽

Structural Development ◽

Regional Economies ◽

Russian Regions ◽

Goods And Services ◽

Border Trade ◽

Regions Of Russia

The purpose of this article is to study border trade in the regions of Russia and the provinces of China. It is shown that in recent years there have been negative trends in border trade, leading to its reduction. Therefore, Russian regions bordering with China should transform into separate objects of regional policy, while also forming a legislative framework for border interaction in all its main spheres: trade, humanitarian cooperation, science and education, and others. In border cooperation with China, special emphasis should be placed on cooperation in the innovation sphere, as well as on improving the quality of exported goods and services and promoting infrastructure projects. Border trade between the regions of Russia and China is built mainly on trade and export of labour resources from China to Russia, as well as China’s receipt of additional sales channels for the confidently growing sales markets for consumer goods and sources of raw materials and primary products. The interaction of Russian regions with neighbouring provinces on the border with China not only preserves the raw material orientation of these regions, but also hinders the development and strengthening of the manufacturing industry in the structure of their regional economies, since border interaction for Russian regions immobilizes those stages of value-added production observed in the very first stages. In general, for the Chinese provinces there is a similar problem associated with such exports to border regions that have common borders with Russia, which does not contribute to the diversification and structural development of the regional economies of the Chinese provinces. That is why building an effective mechanism for border interaction between Russia and China is a strategically important issue for both countries.

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