scholarly journals New Insights into the Relationships between Bacterial Dynamics and Water Quality of Aquaculture Systems Supplemented with Carbon Source and Biofilm Substratum

2021 ◽  
Vol 9 (10) ◽  
pp. 2168
Author(s):  
Er-Meng Yu ◽  
Zhen-Chi Li ◽  
Zhi-Fei Li ◽  
Guang-Jun Wang ◽  
Yun Xia ◽  
...  

Aquaculture is crucial for achieving the FAO’s goal of a world without hunger and malnutrition. Recently, biofilm substratum has been proposed as an effective means to control waste pollution caused by excessive nutrient inputs from aquaculture, but key bacterial communities involved in the remediation remain unclear. Here we reported a freshwater mesocosm study where the addition of biofilm substrata with external carbon effectively controlled the total ammonia nitrogen and improved fish growth. 16S rRNA study and Weighted UniFrac analysis revealed that bacterial compositions were significantly different (999 permutations, p-value < 0.01) between the biofilm-substrata-added and biofilm-substrata-free systems. Planctomycetes were found, as key bacteria benefited from the biofilm substrata addition and exerted the major function of ammonia nitrogen control. Our study demonstrated that the addition of biofilm substrata and an external carbon source favored fish growth and improved the aquaculture environment by the formation of a unique bacteria community.

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1724 ◽  
Author(s):  
Asmaa T.Y. Kishawy ◽  
Alaa H. Sewid ◽  
Hend S. Nada ◽  
Mohamed A. Kamel ◽  
Shefaa A.M. El-Mandrawy ◽  
...  

The aim of the present study was to evaluate mannan oligosaccharides (MOS) or glycerol (GLY) as a carbon source on biofloc systems of Nile tilapia (O. niloticus) juveniles. Fish (n = 750) were reared in open flow (Controls) or biofloc systems (B-GLY and B-MOS) fed with a plant or fish protein source over a period of twelve weeks. Total ammonia nitrogen and nitrate decreased in the biofloc groups, while biofloc volume increased in B-MOS. Compared to the controls, B-MOS and B-GLY exhibited higher weight gain and improved feed conversion, irrespectively of the diet. Serum level of C-reactive protein was reduced, while IgM and lysozyme activity was higher in the B-MOS fish, compared to other groups. Intestinal Bacillus spp. count was increased, whereas Vibrio, Aeromonas and Pseudomonas spp. counts decreased in B-MOS reared groups, compared to the other groups. The proinflammatory cytokine (IL-8 and IFN-γ) transcript expression was upregulated in B-MOS more than B-GLY reared groups. Compared to the controls, the virulence of Aeromonas hydrophila was decreased in the B-MOS and B-GLY groups. The results indicate several benefits of using MOS as a carbon source in a biofloc Nile tilapia system; a cost benefit analysis is required to assess the economic viability of this.


2016 ◽  
Vol 1 (1) ◽  
pp. 39
Author(s):  
Fatmawati Fatmawati ◽  
K Soewardi ◽  
T Kusumastanto ◽  
L Adrianto

The purpose of this study was to estimate the carrying capacity of coastal waters of the pond effluent. The data required for analysis of mass balance models of carrying capacity is based on the total ammonia-nitrogen (NH3-N) concentration in coastal waters. Estimation of carrying capacity with the concept mass balance model using the formula Tchobanoglous (1990) and Predalumpaburt (1996) in Tookwinas (1998) which has been modified by including the formula Widigdo and Pariwono (2001). Carrying capacity of coastal waters of Sebamban Baru Village, Tanah Bumbu Regency maximum pond area calculation may be developed for aquaculture area of 412,583 hectares, this shows that the concentration of ammonia released by the pond has not been an impact on the quality of coastal waters because the existing pond area is new opened an area of 368.542 ha has not exceeded the carrying capacity of the mass balance.


2019 ◽  
Vol 80 (9) ◽  
pp. 1633-1642
Author(s):  
M. Vojtiskova ◽  
B. Satkova ◽  
J. Bindzar ◽  
P. Jenicek

Abstract Postaeration, where digested sludge is introduced into aerobic conditions, is a technology that could improve the quality of sludge and sludge liquor in many ways. Although it is a fairly simple process, only few data about the effect of postaeration have been published. In this study, batch experiments have been performed first, indicating that postaeration affects the total ammonia nitrogen (TAN) and sludge dewaterability. In the removal of TAN, both stripping and biological oxidation can play an important role depending on specific condition. Then the postaeration was investigated in a semicontinuous batch reactor. In addition, the effect of postaeration on the concentration of selected micropollutants such as pharmaceuticals, EOX and AOX was studied. The hydraulic retention time (HRT) of 8, 6, 4 and 2 days and different aeration intensities were tested. The TAN removal efficiency achieved was about 40–60%, sludge dewaterability expressed by sludge cake total solids (TS) concentration after dewatering improved relatively by 5–30%. In addition, TS degradation is also taking place and therefore the reduction of the amount of final sludge to be disposed could be even higher. The biggest changes in observed parameters were recorded at the longest HRT.


2021 ◽  
Vol 34 ◽  
pp. 5
Author(s):  
Lestari L. Widowati ◽  
S. Budi Prayitno ◽  
Sri Rejeki ◽  
Tita Elfitasari ◽  
Pujiono W. Purnomo ◽  
...  

The high organic waste content of river water in Demak, north coast of Java, has caused traditional small-scale pond farmers to stop stocking shrimp. This paper examines whether seaweed and mussel will improve the quality of water these farmers use. The effect of Gracilaria verucosa and Perna viridis on the water quality was assessed by measuring the removal rates (RRs) of total organic material (TOM), total ammonia nitrogen (TAN), nitrite, and nitrate. The specific growth rates (SGRs) of seaweed and mussel were also measured. Thirty-six semi-outdoor tanks containing 800 L of brackish water and 7 cm substrate were randomly assigned to four replications of four densities of G. verucosa: 50 (S50), 100 (S100), 150 (S150), and 200 (S200) g m−2, and of P. viridis: 60 (M60), 90 (M90), 120 (M120), and 150 (M150) g m−2. Weekly, the TOM, TAN, nitrite, and nitrate contents were measured, seaweed and mussel weighted; RRs and SGRs were calculated at the end of the study. The effect of densities on the RRs was significant for both seaweed and mussel. P. viridis was more effective in reducing TOM (by 38%) than G. verucosa (7%); G. verucosa achieved higher RRs for TAN, nitrite, and nitrate. At S200, TOM and TAN decreased by 7.4% and 67%, respectively. At M90, TOM and TAN, decreased by 38% and 49%, respectively. However, nitrite increased significantly at S200 and M150. The SGR of seaweed was significantly lower at S200 than that at S150, S100, and S50. The best performing densities were S100 and M90.


2018 ◽  
Vol 34 (6) ◽  
pp. 908-917 ◽  
Author(s):  
Horacio A Aguirre-Villegas ◽  
Mahmoud A Sharara ◽  
Rebecca A Larson

Abstract. The nutrient profile in stored manure can be highly variable due to the solids building up at the bottom of the storage over time as unagitated manure is removed. This variability can lead to under- or over-application of nutrients potentially reducing crop yields or increasing nutrient losses, respectively. Agitation of stored manure is a common practice to re-suspend solids providing a more uniform nutrient consistency for application. This study explores the solids and nutrient variability in stored dairy manure after agitation and the relationship between the number of samples and the quality of the nutrient content estimate. A total of 16 dairy facilities across Wisconsin were sampled in the study. Samples were taken during agitation and analyzed for total solids (TS), total Kjeldahl nitrogen (TKN), total ammonia nitrogen (TAN), total phosphorus (TP), total potassium (TK), and microminerals. Overall, TKN, TAN, and TP contents were more uniform than TS. The mean concentrations (wet basis) from the 16 farms range from 2.45% to 15.28% for TS, 0.17% to 0.53% for TKN, 0.01% to 0.33 for TAN, 0.02 to 0.06 for TP, and 0.11% to 0.31% for TK. This range is mostly attributed to the between-farms variability in manure nutrient content. In addition, 54% of the total variation in the TS content was attributable to within-farm sample variability. These results show that TS was variable on farms whereas TKN, TAN, and TP were variable between farms. A random resampling analysis showed that three samples generate a mean between 20% to 30% of the true experimental mean for TKN and TAN whereas nine samples are required to be in this range for TP. Results show that the improvement achieved by adding more samples than 11 is less than 10%. Keywords: Dairy manure, Manure agitation, Nutrients, Nutrient variability, Sample size.


2018 ◽  
Vol 41 (1) ◽  
pp. 36493
Author(s):  
Weudes Rodrigues Andrade ◽  
Marielly Maria Almeida Moura ◽  
Vicente Ribeiro Rocha Júnior ◽  
Renê Ferreira Costa ◽  
Luiz Henrique Tolentino Santos ◽  
...  

 This study evaluated the effect of increasing levels of leucaena forage in sorghum: forage. In addition to pure sorghum, it was evaluated different levels of inclusion of leucaena (15, 30 and 50%) at the time of ensiling. Forage was ensiled in PVC tubes (100 mm diameter, 500 mm length) under the density of 500 – 600 kg m-3, a total of 20 silos were prepared, which were opened 35 days after ensiling. There was no effect (p > 0.05) of leucaena inclusion levels for pH, water activity (Aw) and total ammonia nitrogen (total NH3-N). There was an increasing linear effect (p < 0.05) for the content of dry matter (DM), crude protein (CP) and lignin that increased from 33.47 to 34.75%, 7.82 to 16.05% and from 7.29 to 9.93%, at the levels from 0 to 50% inclusion of leucaena, respectively. There was a decreasing linear effect (p < 0.05) for neutral detergent fiber corrected for ash and protein (NDFcp) and hemicellulose, which reduced from 56.65 to 48.37% and from 24.07 to 17.67% at the levels of 0 and 50% inclusion, respectively. For the ash content, there was a quadratic effect (p < 0.05), with a maximum at 8.3% with 8.9% inclusion of leucaena. The inclusion of up to 50% leucaena into sorghum silage is recommended, because, in addition to maintaining the fermentation quality, it also improved nutritional quality of sorghum silage.


2019 ◽  
Vol 9 (4) ◽  
pp. 413-420
Author(s):  
Amella Gusty ◽  
Dachriyanus Dachriyanus ◽  
Leni Merdawati

Kinerja perawat merupakan hasil yang dicapai dalam melaksanakan asuhan keperawatan di rumah sakit. Terciptanya asuhan keperawatan yang optimal sangat diperlukan dukungan dari pihak rumah sakit salah satunya adalah menciptakan kualitas kehidupan kerja baik bagi perawat. Penelitian ini bertujuan untuk mengetahui hubungan antara kualitas kehidupan kerja terhadap kinerja perawat pelaksana di RSUD Teluk Kuantan. Jenis penelitian kuantitatif dengan pendekatan cross sectional. Jumlah sampel 120 responden yang diperoleh melalui proportionate simple random sampling. Hasil penelitian menunjukkan kualitas kehidupan kerja perawat RSUD Teluk Kuantan berada pada kategori sedang, kinerja perawat berada pada kategori kurang baik. Berdasarkan analisis kedua variabel teridentifikasi bahwa tidak ada hubungan antara kualitas kehidupan kerja dengan kinerja perawat pelaksana dengan  p value 0,817. Dimensi work context merupakan komponen yang memiliki hubungan dengan kinerja perawat pelaksana dengan p value 0,008, dimensi work life  tidak berhubungan secara signifikan dengan kinerja perawat. Kesimpulan penelitian diketahui tidak ada hubungan antara kualitas kehidupan kerja dengan kinerja perawat pelaksana. Ada hubungan dimensi work context dengan kinerja perawat pelaksana. Tidak ada hubungan dimensi work life dengan kinerja perawat.   Kata kunci: kualitas kehidupan kerja, kinerja, perawat pelaksana   THE RELATIONSHIP BETWEEN THE QUALITY OF WORK LIFE AND THE PERFORMANCE OF IMPLEMENTERS NURSES   ABSTRACT Nurse performance is the result achieved in implementing nursing care in a hospital. The creation of optimal nursing care is very much needed support from the hospital, one of which is to create a good quality of work life for nurses. This study aims to determine the relationship between the quality of work life and the performance of nurses at the Kuantan District Hospital. This type of quantitative research with cross sectional approach. The number of samples of 120 respondents obtained through proportionate simple random sampling. The results showed that the quality of work life of nurses at the Kuantan District Hospital was in the medium category, the nurses' performance was in the unfavorable category. Based on the analysis of the two variables, it was identified that there was no relationship between the quality of work life with the performance of implementing nurses with a p value of 0.817. The work context dimension is a component that has a relationship with the performance of nurses implementing with p value 0.008, the dimension of work life does not significantly correlate with nurse performance. The conclusion of the research is that there is no relationship between the quality of work life and the performance of the implementing nurses. There is a relationship between the dimensions of the work context and the performance of the nurses. There is no relationship between the dimensions of work life and nurse performance.   Keywords: quality of work life, performance, implementers nurse


2019 ◽  
Vol 11 (1) ◽  
pp. 9-18
Author(s):  
Abdul Wakhid ◽  
Ana Puji Astuti ◽  
Maya Kurnia Dewi

Logoterapi merupakan terapi untuk menemukan makna positif dibalik sebuah kejadian yang tidak diharapkan. Logoterapi dilaksanakan secara individu maupun berkelompok dalam bentuk konseling dan berorientasi pada pencarian makna hidup individu. Tujuan logoterapi meningkatkan makna pengalaman hidup individu yang diarahkan kepada pengambilan keputusan yang bertanggung jawab. Penelitian ini dilakukan dengan menggunakan rancangan pre-experiment dengan metode pre and post test group, artinya pengumpulan data dilakukan terhadap responden untuk membandingkan kualitas hidup sebelum dan sesudah dilakukan intervensi. Teknik pengambilan sampel dilakukan dengan metode total sampling yaitu pengambilan seluruh sampel dengan tetap memperhatikan kriteria yang telah ditetapkan. Jumlah pasien yang menjalani hemodialisis di RSUD Ungaran sebanyak 21 orang dan di RSUD Ambarawa sebanyak 25 pasien. Analisis data dilakukan dengan menggunakan uji t test dependent. Hasil penelitian didapatkan bahwa dari 46 responden didapatkan rata-rata skor kualitas hidup pasien yang mejalani hemodialisis sebesar 60.22 dengan skor terrendah 55 dan skor tertinggi 69. Bahwa dari 46 responden didapatkan rata-rata skor kualitas hidup pasien yang mejalani hemodialisis sebesar 88.72 dengan skor terrendah 79 dan skor tertinggi 103. Hasil uji statistik dengan uji t test dependent diketahui ada pengaruh logoterapi terhadap kemampuan memaknai hidup pada klien yang menjalani hemodialisis di RSUD Kabupaten Semarang (p value: 0,0001). Saran perlunya peningkatan kemampuan perawat dalam memberikan layanan kesehatan termasuk pemberian atau pemanduan penemuan makna hidup bagi pasien hemodialysis, agar selain dengan hemodialysis, ada faktor internal dari pasien yang dapat dijadikan sebagai motivasi untuk sembuh dari penyakit.   Kata Kunci: Logoterapi, kualitas hidup   IMPROVE THE QUALITY OF LIFE OF PATIENTS WITH RENAL FAILURE WHO UNDERWENT HEMODIALYSIS   ABSTRACT Logotherapy is a therapy to discover the positive meaning behind an unexpected event. Logotherapy is carried out individually or in groups in the form of counseling and oriented to the search for the meaning of individual life. This study aims to improve the quality of life of patients with renal failure who underwent hemodialysis. This research was conducted by using pre-experiment with pre-post test study. The sampling technique was done by the convenience sampling. The number of patients undergoing hemodialysis as many as 46 respondents. Data analysis was done by using test t test dependent. The result showed that from 46 respondents got the mean of quality of life of patients who had hemodialysis 60.22 with lowest score 55 and highest score 69. Whereas from 46 respondents got the mean score of life quality of patients who had hemodialysis 88.72 with score the lowest score 79 and the highest score 103. The result of statistical test with t test dependent is known there is influence of logoterapi to the ability of meaningful life on client who undergo hemodialysis at Semarang Regency hospitals (p value: 0.0001). Advice on the need to improve the nurse's ability to provide health services, including the provision or guidance of the discovery of the meaning of life for hemodialysis patients, in addition to hemodialysis, there are internal factors of the patient that can be used as a motivation to recover from illness.   Keywords: Logotherapy, quality of life, kidney failure.  


2020 ◽  
Vol 2 (2) ◽  
pp. 112-120
Author(s):  
Nursari Abdul Syukur ◽  
Susi Purwanti

Many mothers who give birth to Sectio Caesarea (SC) do not Initiate Early Breastfeeding (IMD), which fails exclusive breastfeeding. This study aimed to determine the effect of IMD management in postpartum SC mothers on nutritional status, speed of milk production, and quality of breast milk protein. Method: quantitative research with quasi approach experiment. The research design used was a pre-post-test control non-equivalent control group. A sampling of this study used the Consecutive method sampling with a sample of 20 mothers who gave birth by cesarean section (SC). Hypothesis testing uses the independent t-test and the Mann-Whitney test. The study results showed an influence on the management of IMD in postpartum SC mothers on the speed of ASI production (p-value=0.004) and nutritional status (p-value=0.028). There was no effect of IMD management on postpartum SC mothers on the quality of breast milk protein (p-value = 0.543). This study recommends that the hospital implement an IMD promotion program before the abdominal wall is closed as a form of intervention to increase milk production and maternal nutritional status


2018 ◽  
Vol 5 (2) ◽  
pp. 56-63
Author(s):  
Abdul Wakhid ◽  
Estri Linda Wijayanti ◽  
Liyanovitasari Liyanovitasari

Background: Self efficacy can optimize the quality of life of clients who undergo the healing process due to chronic diseases. Individuals with higher self-efficacy move their personal and social resources proactively to maintain and improve the quality and length of their lives so that they experience a better quality of life. Objectives: the purpose of this study was to find the correlation between self efficacy and quality of life of patients with chronic kidney disease who undergo hemodialysis at RSUD Semarang Regency. Metode: This type of research was descriptive correlation with cross sectional approach. The samples in this study more 76 people with total sampling technique. The data collection tool for self efficacy was measured by General Self-Efficacy scale, for quality of life with WHOQoL-BREF. Statistical test used Kolmogorov-smirnov. Result: The result showed that self efficacy in patients with chronic kidney disease was mostly in moderate category (53,9%), quality of life in patients with chronic kidney disease was mostly in good category (68,4%). There was a correlation between self efficacy and quality of life of patients with chronic kidney disease who undergo hemodialysis at RSUD Semarang Regency, the result obtained p-value of 0.000 <α (0,05). Suggestion: Patients with chronic kidney disease can maintain good quality of life by helping to generate positive self-esteem and high self efficacy.


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