scholarly journals AKTIVITAS SENYAWA ANTIBAKTERI EKSTRAK PAREPAT (Sonneratia alba) TERHADAP PERTUMBUHAN Ralstonia solanacearum DAN Streptococcus sobrinus

2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Saat Egra ◽  
Mardhiana Mardhiana ◽  
Ningrum Indah Rahayu ◽  
Nurjannah Nurjannah ◽  
Sudirman Sirait ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Stem Bark ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Streptococcus Sobrinus ◽  
Mangrove Plant ◽  
Natural Medicine ◽  
Antibacterial Assay ◽  
Sonneratia Alba

AbstractParepat/pidada putih (Sonneratia alba) is a type of mangrove plant that is used by the traditional tribe for natural medicine. This study uses leaves and stem bark extracted with ethanol. Antibacterial assay uses Ralstonia Solanaceaerum and Streptococcus sobrinus by diffusion agar method. The concentrations used were 5000ppm, 10000ppm, 20000ppm with positive control (Chloramphenicol), and negative control (Ethanol 40%). The results of this study obtain the moisture factor of S. alba leaves has a humidity of 0.31 and stem bark of 0.49. The yield showed that the amount of S. alba leaf extract was 23.86% and the bark was 7.31%. S. alba leaf extract was able to inhibit the bacteria R. solanacearum at concentrations of 5000ppm, 10000ppm and 20000ppm with inhibitory values of 27.46%, 34.34% and 37.78%, respectively. While bark extract can inhibit R. solanacearum at concentrations of 5000 ppm, 10000 ppm and 20000 ppm with inhibitory values of 35.38%, 38.47% and 41.92%, respectively. S. alba leaf extract is able to inhibit S. sobrinus bacteria only at concentrations of 10000 ppm and 20000 ppm with inhibitory values of 28.07% and 48.51%. Whereas S. alba bark extract was able to inhibit S. sobrinus at a concentration of 5000 ppm, 10000 ppm and 20000 ppm with inhibitory values of 16.18%, 49.02% and 61.27%. Keywords: Antibacterial, Leave, Stem bark, Mangrove, S. alba AbstrakParepat/pidada putih (Sonneratia alba) merupakan salah satu jenis tumbuhan mangrove yang dimanfaatkan oleh masyarakat sebagai obat tradisional. Penelitian ini menggunakan daun dan kulit batang yang diekstraksi dengan etanol. Pengujian antibakteri menggunakan Ralstonia Solanaceaerum dan Streptococcus sobrinus dengan metode difusi agar sumuran. Kosentrasi yang digunakan yaitu 5000 ppm, 10000 ppm, 20000 ppm dengan kontrol positif (Chloramphenicol), dan kontrol negatif (Etanol 40%). Hasil penelitian ini menunjukkan faktor kelembaban daun S. alba memiliki kelembaban yaitu 0,31 dan kulit batang yaitu 0,49. Rendemen menunjukkan bahwa jumlah ekstrak daun S. alba yaitu 23,86% dan kulit batang 7,31%. Ekstrak daun S. alba mampu menghambat bakteri R. solanacearum pada konsentrasi 5000ppm, 10000ppm dan 20000ppm dengan nilai persentase hambat yaitu masing-masing 27,46%, 34,34% dan 37,78%. Sedangkan ekstrak kulit batang mampu menghambat R. solanacearum pada konsentrasi 5000 ppm, 10000 ppm dan 20000 ppm dengan nilai persentase hambat secara berturut 35,38%, 38,47% dan 41,92%. Ekstrak daun S. alba mampu menghambat bakteri S. sobrinus hanya pada konsentrasi 10000 ppm dan 20000 ppm dengan nilai persentase hambat yaitu 28,07% dan 48,51%. Sedangkan ekstrak kulit batang S. alba mampu menghambat S. sobrinus pada konsentrasi 5000 ppm, 10000 ppm dan 20000 ppm dengan nilai persentase hambat yaitu 16,18%, 49,02% dan 61,27%. Kata kunci: Antibakteri, Daun, Kulit bantang, Mangrove, S. alba.

scholarly journals Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia pinnata) terhadap Bakteri Staphylococcus aureus secara In vitro

Jurnal MIPA ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 128 ◽  
Author(s):  
Mercy Ngajow ◽  
Jemmy Abidjulu ◽  
Vanda S. Kamu
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolites ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Distilled Water

Telah dilakukan penelitian secara kualitatif terhadap pengaruh antibakteri dari ekstrak kulit batang matoa (Pometia pinnata. Spp.) terhadap bakteri Gram positif Staphylococcus aureus . Sebelum dilakukan uji antibakteri, sampel yang telah diekstrak secara maserasi diuji fitokimia terlebih dahulu untuk menentukan kandungan metabolit sekunder yang telah diketahui berperan sebagai agen antibakteri. Setelah dilakukan uji fitokimia, ekstrak diuji aktivitas antibakterinya terhadap bakteri Staphylococcus aureus dengan menggunakan teknik difusi agar dengan cara sumuran. Ekstrak dilarutkan pada aquades steril dengan perbandingan 2 g ekstrak pada 2 mL air. Untuk kontrol positif, digunakan ciprofloxacin dan aquades steril sebagai kontrol negatif. Hasil penelitian ini menunjukkan bahwa ekstrak kulit batang matoa memiliki aktivitas antibakteri setelah diinkubasi selama 24 jam pada 37 OC. Dari 3 kali pengulangan dengan masing – masing 3 sumuran, didapat zona hambat masing – masing 16.84 mm, 12.5 mm dan 14.5 mm dengan kontrol positif 29.67 mm serta kontrol negatif 0 mm. Hasil yang diperoleh didukung oleh keberadaan metabolit sekunder hasil uji fitokimia yaitu tanin, flavonoid, terpenoid dan saponin.A qualitative study has done  of the antibacterial effect of matoa (Pometia pinnata) stem bark extract against Gram-positive bacteria Staphylococcus aureus . Before the antibacterial test , samples were extracted by maceration and then  phytochemical  tested to measuring the content of secondary metabolites  that have been known to act as an antibacterial agent . After being tested of phytochemical , extracts were tested the antibacterial effect  against Staphylococcus aureus using agar diffusion technique. Extract was dissolved in sterile distilled water with a ratio of 2 g of extract in 2 mL of water . For the positive control , use of ciprofloxacin and sterile distilled water as a negative control . Results of this study indicate that matoa bark extract has antibacterial effect after incubation for 24 h at 37OC . Of 3 times with each repetition - each 3 wells, the inhibition zone obtained - each 16.84 mm , 12.5 mm and 14.5 mm with 29.67 mm of positive control and a negative control by 0 mm . The results are supported by the presence of secondary metabolites by phytochemical test such as tannins , flavonoids , terpenoids and saponins.

scholarly journals Pengaruh ekstrak daun binahong (Anredera cordifolia ) terhadap pemulihan siklus estrus mencit (Mus musculus)

2021 ◽  
Vol 10 (2) ◽  
pp. 32
Author(s):  
Diana Novita Sari ◽  
Ramadhan Sumarmin ◽  
Fitri Arsih ◽  
Fitra Arya Dwi Nugraha
Keyword(s):  
Estrous Cycle ◽  
Analysis Of Variance ◽  
Mus Musculus ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Vaginal Smear ◽  
Level Of Significance ◽  
Range Test

This study aimed to determine the effect of binahong (Anredera cordifolia) leaf extract on the recovery of the estrous cycle of mice (Mus musculus) which has been induced for the extension of the estrous cycle using angsana (Pterocarpus indicus) bark extract. In this study vaginal smear was performed daily at 10.00 WIB to determine the phase of the estrous cycle for 26 days. Mice were divided into 5 groups, each with 6 replicates. The treatments were T1 (positive control), T2 (negative control), T3 (0.8 mg/head/day), T4 (2.4 mg/head/day) and T5 (4 mg/head/day). The data obtained were processed using Analysis of Variance (ANOVA) and followed by the Duncan's New Multiple Range Test (DNMRT) at a 95% level of significance.  The results showed that the angsana (Pterocarpus indicus) bark extract adminsitration (T2) lengthen (p <0.05) the estrous cycle of mice compared to those of normal mice (T1). The administration of binahong (Anredera cordifolia) leaf extract on T3 and T4 groups mice were followed by shorter (p <0.05) estrous cycle than those of T2, and it were not significantly different (p >0.05) compared to T1 group. However, the higher dose of binahong (Anredera cordifolia) leaf extract (T5) revealed the length of estrous cycle was not significantly different (p >0.05) compared to T2 group. It can be concluded that the administration of binahong leaf extract was able to recover the normal estrous cycle of mice.

UJI IMUNOMODULATOR DARI EKSTRAK ETANOL DAUN WARU (HIBISCUS TILIACEUS) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT

2019 ◽  
Vol 1 (2) ◽  
pp. 11-16
Author(s):  
Yanna Rotua Sihombing ◽  
Debi Dinha Sitepu
Keyword(s):  
Treatment Group ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Delayed Type Hypersensitivity ◽  
Hypersensitivity Response ◽  
Delayed Type Hypersensitivity Response

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno®  32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno®  32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

scholarly journals Effect of Carica papaya L. Stem Bark Extracts on Cholesterol Concentration in Rats Induced with Streptosozin

2020 ◽  
Vol 151 ◽  
pp. 01011
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Body Weight ◽  
Carica Papaya ◽  
Stem Bark ◽  
Negative Control ◽  
Diabetic Rats ◽  
Cholesterol Concentration ◽  
Bark Extract ◽  
Treatment Groups ◽  
Randomized Design ◽  
Stem Bark Extract

This study was designed to determine the effect of Carica papaya L. stem bark extracts on cholesterol concentration in rats induced with glibenclamide. A completely randomized design was used for the experiment which consisted of 6 treatment groups, each group consisted of four rats, as follows:1) KN (negative control, non-diabetic rats); KP, diabetic rats given glibenclamide 10 mg/kg body weight; EP 1, diabetic rats given 0 mg/kg body weight/day extract; EP2, diabetic rats given 100 mg/kg body weight/day extract; and EP3, diabetic rats given 200 mg/kg body weight/day extract, EP4, diabetic rats given 300 mg/kg body weight/day extract for 28 day. The results showed that C. papaya L. stem bark extract decreased (P<0.05) cholesterol levels in diabetic rats. It was concluded that C. papaya L. stem bark extract had potential as anti-hypercholesterolemic in diabetic rats.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Uji daya hambat ekstrak daun awar-awar (ficus septica burm.f) terhadap pertumbuhan bakteri staphylococcus aureus dan eschericia coli

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Ismiranti D.A. Tuna ◽  
Pemsi M. Wowor ◽  
Henoch Awaloei
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infection ◽  
Natural Science ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
University Faculty ◽  
Microbiology Laboratory ◽  
Causal Organism ◽  
Empiric Data

Abstract: Awar-awar (Ficus septica Burm.F) is one of the plants that used by comunities. Based from the empiric data, this plant is believed have efficacy as a drug and could treat diseases. Staphylococcus aureus dan Eschericia coli are the most frequent causal organism in human bacterial infection. This study aimed to determine whether there is inhibition of leaf extract awar-awar against Staphylococcus aureus and Eschericia coli. This was a laboratory experimental with Kirby-Bauer pitting modification at Phytochemistry and Microbiology Laboratory of Sam Ratulangi University Faculty of Mathematics and natural Science. Awar-awar leaf extract was obtained from maceration process with 96% ethanol. Consentration of thick extract that was used were 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml. ciprofloxacin was used as positive control,and aquades was used as negative control. The result showed that the average of inhibition diemeters by awar-awar leaves were 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml respectively are 27,5 mm, 24,3 mm, 22,3 mm, 21,8 in Staphylococcus aureus, whereas in Eschericia coli were 24,8 mm, 21 mm, 18,3 mm, 17 mm. Conclusion: Awar-awar leaf extract has a potential inhibion effect on the growth of Staphylococcus aureus and Eschericia coli.Keywords: awar-awar leaves extract, staphylococcus aureus, eschericia coli, inhibition test. Abstrak: Tanaman awar-awar merupakan salah satu tanaman yang digunakan masyarakat untuk pengobatan. Berdasarkan data empiris, tanaman ini dipercaya memiliki khasiat sebagai obat dan bisa mengobati penyakit. Bakteri Staphylococcus aureus dan Eschericia coli merupakan penyebab infeksi utama pada manusia. Penelitian ini bertujuan untuk mengetahui ada tidaknya daya hambat ekstrak daun awar-awar terhadap bakteri Staphylococcus aureus dan Eschericia coli. Jenis penelitian ini ialah eksperimental laboratorium dengan modifikasi Kirby-Bauer sumuran di Laboratorium Fitokimia dan Mikrobiologi Fakultas MIPA Universitas sam Ratulangi. Ekstrak daun awar-awar diperoleh dari proses maserasi dengan etanol 96%. Konsentrasi ekstrak kental yang digunakan dalam penelitian ini ialah 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml. Siprofloksasin sebagai kontrol positif, dan akuades sebagai kontrol negatif. Hasil penelitian mendapatkan rata-rata diameter zona hambat ekstrak daun awar-awar konsentrasi 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml berturut-turut ialah 27,5 mm, 24,3 mm, 22,3 mm, 21,8 mm pada bakteri Staphylococcus aureus, sedangkan pada bakteri Eschericia coli ialah 24,8 mm, 21 mm, 18,3 mm, 17 mm. Simpulan: Ekstrak daun awar-awar berpotensi memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dan Eschericia coli. Kata kunci: ekstrak daun awar-awar, staphylococcus aureus, eschericia coli, uji daya hambat.

scholarly journals UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 44-53
Author(s):  
Andri Priyoherianto ◽  
◽  
Panji Ratih Suci ◽  
Putri Rizki Cahya Fatimah ◽  
Atik Nur Wijayanti
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Water Hyacinth ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Normal Limits ◽  
Blood Glucose Levels ◽  
Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

In vitro Anthelminthic Activity of Crude Methanolic Leaf Extract of Hyptis suaveolens (L.) Poit (Bush mint) on Ascaris suum.

2019 ◽  
Vol 2 (1) ◽  
pp. 82-87
Author(s):  
O. S Salami
Keyword(s):  
Leaf Extract ◽  
Ascaris Suum ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Analysis ◽  
Egg Hatchability ◽  
Anthelminthic Activity ◽  
Life Cycle Stages ◽  
Hyptis Suaveolens

Ascariasis is a neglected tropical disease prevalent in areas with poor hygiene and low standard of living in tropical countries. Hyptis suaveolens is been used traditionally around the world for various ailments. The aim of the study was to evaluate the anthelminthic activity of the methanolic leaf extract of the plant on the three life cycle stages of Ascaris suum synonymous to Ascaris lumbricoides. The phytochemical analysis of the extract was evaluated. The in-vitro anthelminthic activity of the methanolic leaf extract of Hyptis suaveolens on the egg hatchability and larva inhibition of Ascaris suum were assessed using Bizimenyara inhibition method, where 100 eggs were counted and incubated in 5 different plate-wells containing positive control, negative control and 3 concentrations (50, 70 and 100 mg/ml) of extract. The plate wells were brought out after incubation and content examined under the microscope. The effect of the methanolic leaf extract and pyrantel pamoate on cuticle peeling, motility and mortality were evaluated using adult worms. The egg hatchability and larval development after incubation for 48 h and 21 days significantly (p<0.05 for both) inhibited with 98% unhatched eggs and 95% undeveloped larva of A. suum respectively. There was significant (p<0.05) visible peeling of cuticles, reduced motility and mortality of the adult worms after 30, 12 and 80 minutes respectively. It can be concluded that H. suaveolens has anthelminthic activity on A. suum which may be attributed to some of the phytochemical constituents.

Sign in / Sign up

Export Citation Format

Share Document