Efektivitas Antibakteri Minyak Atsiri Rimpang Jahe Merah (Zingiber officinale var. Rubrum) Terhadap Bakteri Jerawat

2016 ◽  
Vol 6 (1) ◽  
pp. 44
Author(s):  
Nilda Lely ◽  
Arie Firdiawan ◽  
Septiani Martha
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Zingiber Officinale ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Clear Zone ◽  
Propionibacterium Acne ◽  
B Test

A research to investigate antibacterial activities of the essential oil of Zingiber officinale var. Rubrum toward acne bacterials has been done. This study was aimed to compare antibacterial activities of the essential oil toward the growth of some bacteria contributing in acne formation. The separation of the essential oil was done by using destilation process method. From the separation, rendement of essential oil in Zingiber officinale var. Rubrum was 0.182% (v/b). Test of antibacterial activities of the essential oil was conducted using the agar diffusion method toward Propionibacterium acne, Pseudomonas aeruginosa, Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis in some concentrations including 5%, 10%, 15% and 20%. As result, higest concentration of the esssential oil (20%) resulted in biggest activities of Zingiber officinale var. Rubrum toward the bacterial growth marked by diameter of clear zone in the growth medium. The average of diameter of clear zone resulted from 20% essential oil was 20.1mm in Staphylococcus epidermidis; 19.3mm in Propionibacterium acne; 18.4mm in Pseudomonas aeruginosa and 13.8mm in Staphylococcus aureus ATCC 25923.

scholarly journals The Antibacterial Effectiveness of Red Ginger (Zingiber Officinale Roscoe) Essential Oil in Inhibiting The Growth of Staphylococcus Aureus and Streptococcus Mutans

2021 ◽  
Vol 7 (2) ◽  
pp. 364-373
Author(s):  
Shavilla Lukita ◽  
Winda Khosasi ◽  
Chandra Susanto ◽  
Florenly
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Zingiber Officinale ◽  
Diffusion Method ◽  
Ginger Extract ◽  
Zingiber Officinale Roscoe ◽  
Red Ginger ◽  
Post Hoc

Red ginger extract has a category strong antibacterial effect on Staphylococcusaureus and Streptococcus mutans. Red ginger essential oil has the potential forstronger inhibition. This study aims to compare the antibacterial effectiveness of redginger essential oil against Staphylococcus aureus and Streptococcus mutans. Thedesign of this study was a laboratory experimental design with a factorial completelyrandomized design. The red ginger used in this study was proven to be a species ofZingiber officinale Roscoe. The production of essential oils in this study uses thesteam distillation method. The content of secondary metabolites in red ginger wastested quantitatively by the GC-MS method. Determination of antibacterial activityusing the disc diffusion method. The data were processed using the SPSS 21.0program. The normality of data distribution was tested with the Shapiro-Wilk test,followed by one-way ANOVA, Levene's test, and the Tukey HSD Post Hoc Test. Theresults of the antibacterial test of red ginger essential oil against Staphylococcusaureus (21.21mm ± 0.315) and Streptoococcus mutans (23.43mm ± 0.189) provedthat the inhibition power of the category was very strong at a concentration of 75%.

scholarly journals Antibacterial of Cinnamon Bark (Cinnamomum burmannii) Essential Oil Against Methicillin-Resistant Staphylococcus aureus

2021 ◽  
Vol 4 (1) ◽  
pp. 56
Author(s):  
Shinta Levea Ni'matul Fadlilah ◽  
Mustofa Helmi Effendi ◽  
Wiwiek Tyasningsih ◽  
Lucia Tri Suwanti ◽  
Jola Rahmahani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Cinnamon Bark ◽  
Cinnamomum Burmannii

This study aimed to determine the in vitro antibacterial activities of essential oil from cinnamon bark (Cinnamomum burmannii) on Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk. Essential oil from cinnamon bark obtained from the steam distillation method and essential oil was made in a series dilution with a concentration of 1%, 2%, 4%, and 8%. The antibacterial activities were tested using the disk diffusion method. Results showed from five isolates of MRSA, one isolate was sensitive of essential oil with a concentration 2% and all of the isolate was sensitive with a concentration 4% and 8%. The antibacterial activity showed by inhibition zones on  MRSA. The results suggest that the activity of the essential oils of cinnamon bark has an antibacterial effect on MRSA and it is developed as phytopharmaca.

scholarly journals Comparative evaluation of antibacterial activity of solvent fractions and essential oil of Cyperus rotundus L. rhizomes

2021 ◽  
Vol 10 (3) ◽  
pp. 162-165
Author(s):  
Thuvaragan S ◽  
◽  
Nishadini NVC ◽  
Gnanakarunyan T ◽  
Srikaran R ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Cyperus Rotundus ◽  
Diffusion Method ◽  
Ether Extract ◽  
Significant Difference

Cyperus rotundus L. belongs to the family Cyperaceae. It is used to treat constipation, stomach disorders, dysmenorrhea, skin diseases, wounds, leprosy, fever, etc., in indigenous medicine. Also, they are used to treat itchy pus-filled pimples in Sri Lanka. This study aimed to evaluate in vitro antibacterial activity of different solvent fractions and essential oil of Cyperus rotundus L. rhizomes. Soxhlet apparatus was used to obtain the methanolic and petroleum ether extracts from rhizomes, and essential oil was extracted using steam distillation. The antibacterial activity of extracts and essential oils was determined against Staphylococcus aureus and Pseudomonas aeruginosa using the agar disc diffusion method. The activity was compared with Gentamycin as standard. The activity of extracts and essential oil were compared with one-way ANOVA followed by Dunnett's test. The results showed that both extracts and essential oil of Cyperus rotundus L. rhizomes showed a statistically significant difference in antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa compare to the standard drug, Gentamycin. The essential oil of Cyperus rotundus L. rhizomes showed comparatively higher antibacterial activity than extracts. Staphylococcus aureus was more susceptible to methanolic extract and essential oil than petroleum ether extract. Pseudomonas aeruginosa was more susceptible to essential oil and petroleum ether extract than methanol extract. Cyperus rotundus L. rhizomes possess good antibacterial activity, and further studies are required to isolate active phytochemicals and screen for antimicrobial activity

scholarly journals ANTIBACTERIAL POTENTIAL OF JAMU INGGU AGAINTS SOME BACTERIA CAUSING UPPER RESPIRATORY TRACT INFECTION

2015 ◽  
Vol 20 (2) ◽  
Author(s):  
Nunung Sulistyani ◽  
Ardina Nugrahani
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Respiratory Infections ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Upper Respiratory Tract ◽  
Clear Zone ◽  
Bacteria Growth ◽  
Growth Inhibition Zone ◽  
Upper Respiratory

This study aims to examine the potential of jamu inggu as an alternative antibacterial herb that can be used against Staphylococcus aureus, Streptococcus pnuemoniae, S. Pyogenes, and Pseudomonas aeruginosa that is causing Upper Respiratory Infections (URI). Antibacteria activity test of jamu inggu was using the Agar diffusion method by Kirby-Bauer. To test jamu inggu antibacterial activity against Staphylococcus aureus,Streptococcus pnuemoniae, S. Pyogenes, and Pseudomonas aeruginosa, the diameter of the test bacteria growth inhibition zone was measured. The formation of a clear zone around the disc indicates that jamu inggu has chemical compounds that are antibacterial against Staphylococcus aureus,while the Pseudomonas aerugenosa, Streptococcus pyogenes, and S. pneumoniae bacteria cannot be inhibited by jamu inggu. It was indicated by no formation of a clear zone around the disc. Result shows that jamu inggu can be used as an alternativesource of antibiotics against the Staphylococcus aureus

scholarly journals Aktivitas Antibakteri Minyak Atsiri Kulit Kayu Akway (Drimys piperita Hook. f.) pada Beberapa Tingkat Konsentrasi, Keasaman (pH) dan Kandungan Garam

2019 ◽  
Vol 8 (4) ◽  
pp. 149
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Isak Silamba
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Sodium Chloride ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Chloride Content ◽  
Antibacterial Activities ◽  
Water Distillation

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri minyak atsiri kulit kayu akway pada beberapa tingkat konsentrasi, keasaman (pH) dan kandungan sodium klorida. Minyak atsiri disuling dengan menggunakan metode distilasi air. Pengujian aktivitas antibakteri minyak atsiri pada beberapa tingkat konsentrasi, pH dan kandungan sodium klorida dilakukan dengan menggunakan metode difusi sumur. Hasil penelitian menunjukkan bahwa aktivitas antibakteri minyak atsiri kulit kayu akway cenderung meningkat dengan meningkatnya konsentrasi. Konsentrasi penghambatan minimum terhadap Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa dan Staphylococcus aureus adalah 0,28–0,56%. Tingkat keasaman dan kandungan sodium klorida tidak berpengaruh nyata terhadap aktivitas antibakteri minyak atsiri kulit kayu akway. Kesimpulannya, minyak atsiri kulit kayu akway berpotensi sebagai sumber antibakteri alami untuk menghambat pertumbuhan bakteri yang tahan terhadap antibiotik.Antibacterial Activity of Essential Oil of Akway (Drimys piperita Hook f.) Barks on Some Levels of Concentration, Acidity (pH) and Salt ContentsAbstractAkway (Drimys piperita Hook. f) was an aromatic plant of winteraceae. Leaves and barks of this plant contain essential oil. Previous studies indicated that essential oil from some aromatic plants had strong antibacterial activities. The aims of the study were to know antibacterial activities of essential oil isolated from akway bark on some levels of concentration, acidity (pH) and sodium chloride content. The essential oil was distilled by using water distillation method. The antibacterial activity was assayed on several levels of concentration, pH and sodium chloride concentrations that were performed using method of agar well diffusion. The results showed that the antibacterial activity of akway barks essential oil tended to increase with increasing of concentrations. The minimum inhibition concentrations against Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus were 0,28–0,56%. The pH and sodium chloride contents had not significantly influenced to the antibacterial activities of akway barks essential oil. As conclusion, the essential oil of akway barks had potential as source of antibacterial on inhibiting growth of antibiotic resistance bacteria. 

Composition and Antimicrobial Activity of the Essential Oil of Dicyclophora persica Boiss. from Iran

2006 ◽  
Vol 61 (5-6) ◽  
pp. 315-318 ◽  
Author(s):  
Peyman Salehi ◽  
Ali Sonboli ◽  
Fatemeh Mohammadi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Strong Inhibition ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Total Oil

The chemical composition of the essential oil of Dicyclophora persica Boiss. was identified by GC and GC-MS analysis. The analysis of the oil resulted in the identification of forty-five components constituting 98.6% of the total oil. The main constituents were α-pinene (31.5%), (Z)-β-ocimene (23.3%), p-cymene (6.7%) and (E)-β-ocimene (5.4%). The antimicrobial activity of the oil was tested by the disk diffusion method against four Gram-positive (Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis and Enterococcus faecalis) and three Gram-negative (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) bacteria together with a fungus (Aspergillus niger). The oil showed strong inhibition activity toward all the tested microorganisms except for Pseudomonas aeruginosa.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK MIKROALGA Thalassiosira sp TERHADAP BAKTERI Staphylococcus aureus, Staphylococcus epidermidis dan Propionibacterium Acne

2019 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Vina Juliana Anggraeni ◽  
Titis Setyaning Wahyu ◽  
Herni Kusriani ◽  
Dewi Kurnia
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Marine Biota ◽  
Disc Diffusion ◽  
Propionibacterium Acne ◽  
X 24

AbstrakPengembangan obat dan kosmetik dari biota laut kini tengah terjadi di dunia farmasi. Mikroalga Thalassiosira sp merupakan jenis mikroalga yang memiliki kandungan senyawa-senyawa bioaktif. Beberapa penelitian sebelumnya menunjukkan bawa mikroalga memiliki aktivitas antibakteri.  Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari ekstrak mikroalga Thalassiosira sp  terhadap 3 bakteri  yaitu staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne di fasa n-heksan, etil asetat dan etanol. Ketiga bakteri ini dapat menyebabkan infeksi kulit. Mikroalga Thalassiosira sp dikultivasi menggunakan medium walne dan di panen pada hari ke-6 setelah penanaman. Pemanen mikroalga dilakukan dengan teknik sentrifuga. Ektrak dilakukan dengan cara  maserasi bertingkat selama 3 x 24 jam. Pengujian antibakteri dilakukan dengan metode difusi kertas cakram atau metode disc diffusion menurut Kirby-Bauer. Hasil ekstrak mikroalga Thalassiosira sp diperoleh paling banyak pada ekstrak etanol sebanyak 24,24%(b/b), ektrak etil asetat sebanyak 19,75%(b/b) dan paling sedikit adalah ekstrak heksan sebanyak 8,64% (b/b). Hasil uji difusi menunjukkan ekstrak n-heksan, etil asetat dan etanol mikroalga Thalassiosira sp memiliki aktivitas terhadap bakteri staphylococcus aureus, staphylococcus epidermidis dan propionibakterium Acne yang ditunjukan dengan adanya zona bening. Kata kunci: mikroalga, Thalassiosira sp, antibakteri, infeksi kulit, metode difusi Abstract Development drugs and cosmetics from marine biota is now being happened in pharmacy word. Microalgae Thalassiosira sp is a type of microalgae that has a bioactive compounds. Several previous studies have shown the existence of microalgae which have antibacterial activity. This study aimed to study the antibacterial activity of extracts of microalgae Thalassiosira sp against 3 bacteria which is staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne in the n-hexane, ethyl acetate and ethanol phases. These three bacteria can cause skin infections. Microalgae Thalassiosira sp was cultivated using walne medium and harvested on the 7th day after planting. Microalgae harvesters are carried out by centrifuge techniques. The extract is done by multilevel maceration for 3 x 24 hours. Antibacterial testing was carried out by the paper diffusion method or Kirby-Bauer's disc diffusion method. The results of the crude extract of microalgae Thalassiosira sp were obtained at most in ethanol extract as much as 24.24% (w/w), extract of ethyl acetate at 19.75% (w / w) and at least hexane extract at 8.64% (w/w). The diffusion test results for n-hexane, ethyl acetate, and ethanol microalgae Thalassiosira sp extract have activity on  staphylococcus aureus, staphylococcus epidermidis and propionibacterium acne which are supported by clear zones. Keywords: mikroalge, Thalassiosira sp, antibacteria, skin infection, diffusion method

scholarly journals Antibacterial Activity of Honey on Staphylococcus aureus and Streptococcus pyogenes Isolated from Wound Pus

2020 ◽  
Vol 2 (1) ◽  
pp. 38
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Honey Sample ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Test Organisms ◽  
Treatment Of Wounds ◽  
Agar Well Diffusion Method

Antibacterial activity of honey obtained from an apiculture farm was tested on Staphylococcus aureus and Streptococcus pyogens isolated from wound pus was observed. Agar well diffusion method was used to determine the antibacterial activity of honey on the test organisms. Moreover, the result was that the honey sample had heavy antibacterial activities against the test organisms, which was revealed by the zone of inhibition obtained. The antibacterial activity increased with the increase in concentrations of honey and showed a clear zone for both organisms. Therefore honey can be accepted as an antibacterial agent for the treatment of wounds, burns, bedsores, and ulcers.

scholarly journals Evaluation of the Antibacterial Activity, Acute Toxicity and Immuno-stimulatory Potential of Adenodolichos paniculatus root Extract

2020 ◽  
Vol 9 (5) ◽  
pp. 318-322
Author(s):  
IF Kyahar ◽  
◽  
AE Onwuliri ◽  
JO Ehinmidu ◽  
PO Oladosu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Large Population ◽  
Herbal Medicines ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Root Extract ◽  
Immunostimulatory Activity ◽  
Test Organisms

Medicinal plants have been used in the treatment of illnesses from time immemorial. In Nigeria today a large population of people still depend on herbal medicines for their health needs. The medicinal plant, Adenodolichos paniculatus has been used traditionally as a remedy for mouth and throat infections in Pushit community of Plateau state, Nigeria. Five solvents (hexane, chloroform, ethyl acetate, methanol and water) were used sequentially for the isolation of bioactive compounds from the root of the plant Adenodolichos paniculatus and concentrated on rotary vacuum evaporator. Concentrated extracts were tested for their antibacterial activities against Streptococcus pyogenes, Staphylococcus aureus, Pseudomonas aeruginosa and E. coli by agar-well diffusion method. Ofloxacin was the referenced drug. Chloroform root extract was more potent than the remaining four extracts. The extract at 100 mg/ml was active against only three (3) of the four (4) pathogens tested namely Streptococcus pyogenes, Staphylococcus aureus and Pseudomonas aeruginosa with zones of inhibition measuring 25.00, 21.00 and 11.75 mm respectively. The minimum inhibitory concentration of the extract against the test organisms were 1,560, 6,250 and 25,000 µg/ml respectively. The minimum bactericidal concentration was 3,120, 12,500 and 50,000 µg/ml against the three organisms. The extract was found to be nontoxic with LD50 of above 2000 (mg//kg body weight) when administered orally in mice. The plant extract has immunostimulatory activity with a significant increase (p<0.05) in white blood cell proliferation when administered into mice at doses of 50, 25 and 100 mg/kg. Conclusion: The result of this investigation supports the use of this plant for the traditional remedy of mouth and throat infections possibly caused by these test organisms. The extract can serve as lead in phytomedicine development for the treatment of throat related infections.

Antibacterial Activity Test of Various Fraction of Lick leaves (Villebrunea rubescens) against Staphylococcus aureus and Staphylococcus epidermidis Bacterial

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Rani Dewi Pratiwi ◽  
Rani Dewi Pratiwi ◽  
Rani Dewi Pratiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
Water Fraction ◽  
Activity Test ◽  
And Staphylococcus Aureus

Lick leaves (Villebrunea rubescens) are one of the medicinal plants from the Urticaceae family. In Papua, we called it Daun jilat, which is used empirically by the community to treat contusion or bruises in the Serui area, Yapen Island Regency, Papua, Indonesia. Based on the result of the phytochemical screening, V. rubescens contained Alkaloids, Tannins, Saponins, Quinones, and Flavonoids. In previous research on an extract of Lick leaves, it was found that the antibacterial activities within inhibition zone have value of 8.7 mm (125 ?g/mL). This research aims to find out the antibacterial activities of the various fraction of Lick leaves in inhibiting the growth of Staphylococcus epidermidis and Staphylococcus aureus bacterial and to find out the most effective concentration of the various fraction of Lick leaves. The method used in this research was disc diffusion method and the solution used was ethanol-water, ethyl acetate, and N-hexane with the variable concentration used was 100, 250, 500, 750, 1000ppm. The result showed that Lick leaves fraction of ethanol-water, ethyl acetate, and n-hexane had an inhibition zone against the growth of Staphylococcus epidermidis bacteria, while the n-hexane and ethyl acetate had an inhibition zone against the growth of Staphylococcus aureus bacteria. However, for the ethyl-acetate, the inhibition zone was categorized as weak. The concentration of Lick leaves which effectively inhibited the growth of bacteria was ethanol-water fraction with an inhibition zone of 7.78 mm against Staphylococcus epidermidis bacteria and ethanol-water fraction with an inhibition zone of 3.89 mm against Staphylococcus aureus bacteria.

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