Network pharmacology approach to bioactive chemical compounds identified from Lespedeza bicolor lignum methanol extract by GC–MS for amelioration of hepatitis

Gene Reports ◽  
2020 ◽  
Vol 21 ◽  
pp. 100851
Author(s):  
Ki Kwang Oh ◽  
Md. Adnan ◽  
Dong Ha Cho
Keyword(s):  
Methanol Extract ◽  
Chemical Compounds ◽  
Network Pharmacology ◽  
Lespedeza Bicolor

scholarly journals GC-MS analysis and insecticidal effect of methanol extract of Pistacia khinjuk Stocks leaves

2019 ◽  
Vol 113 (2) ◽  
pp. 231
Author(s):  
Sultan RAVAN ◽  
Abbas KHANI ◽  
Nahid VEYSI
Keyword(s):  
Methyl Bromide ◽  
Methanol Extract ◽  
Chemical Compounds ◽  
Environmental Issues ◽  
Contact Toxicity ◽  
Oryzaephilus Surinamensis ◽  
Meoh Extract ◽  
Insecticidal Effect ◽  
Pistacia Khinjuk ◽  
Phenyl Ethyl Alcohol

<p>Regarding the significance of environmental issues, the use of herbal compounds is proposed as an alternative method for methyl bromide and phosphine. In this research, the insecticide effect of MeOH extract of <em>Pistacia khinjuk</em> leaves on <em>Tribolium confusum</em> (Jacquelin du Val, 1863) and <em>Oryzaephilus surinamensis</em> (Linnaeus, 1758) was studied. The chemical compounds of the extract were also identified by the GC-Mass device. The contact toxicity test was performed on 1 to 7-day-old insects. LC<sub>50</sub> values of 9.32 and 5.47 mg ml<sup>–1</sup> were calculated for <em>T. confusum</em> and <em>O. surinamensis</em>, respectively. More than 39 compounds were identified in MeOH extract of <em>P. khinjuk</em>, with 5-ethoxy-4-phenyl-2-isopropylphenol (29.02 %), phenyl ethyl alcohol (10.78 %), benzyl alcohol (7.8 %) and 1, 2-benzenediol (6.67 %) as main compounds. In addition, there were the known insecticide compounds such as spathulenol (2.07 %), myrcene (2.03 %), ρ-cymene (1.67 %), apiol (1.61 %), borneol (0.79 %), and pulegone (0.44 %) in the plant extract that confirmed the potential of the <em>P. khinjuk</em> extract in controlling stored-product insects.</p>

scholarly journals Steroid Compounds From Gynura pseudochina (Lour) DC

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 8 ◽  
Author(s):  
Ferlinahayati Ferlinahayati ◽  
Roby Pahala J Gultom ◽  
Herlina Herlina ◽  
Eliza Eliza
Keyword(s):  
Traditional Medicine ◽  
Methanol Extract ◽  
Chemical Compounds ◽  
Chromatographic Techniques ◽  
Spectral Evidence ◽  
First Time

Daun dewa (Gynura pseudochina Lour DC) is a one of popular traditional medicine to treat various diseases. This research was conducted to isolate chemical compounds from daun dewa leaves using various chromatographic techniques. A steroid mixture namely b-sitosterol (1a) and stigmasterol (1b) were isolated for the first time from the methanol extract of daun dewa. The structures were determined base on spectral evidence including IR, NMR 1D and NMR 2D.

scholarly journals Antimicrobial Activity of Hand Lotion of Flower Mimusops elengi

2019 ◽  
Vol 7 (22) ◽  
pp. 3748-3756
Author(s):  
Bastian Arifin ◽  
Rosnani Nasution ◽  
Novi Desrianti ◽  
Marianne Marianne ◽  
Hira Helwati
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Chemical Compounds ◽  
Positive Control ◽  
Gas Chromatography Mass Spectrometry ◽  
Tropical Region ◽  
Organoleptic Properties ◽  
Flower Extract ◽  
Mimusops Elengi ◽  
Antibacterial And Antifungal

BACKGROUND: Aceh is a tropical region that is very many overgrown by various plants that have medicinal properties; one of them is M. elengi. M. elengi flower extract has the main content of triterpene and alcohol, that have antibacterial, antifungal, antioxidant and antineoplastic activity. Extraction of chemical compounds containing essential oils generally uses distillation, and get the small amounts of chemical compounds, while the maceration with n-hexane solvent, producing less active nonpolar compounds against S. aureus bacteria. AIM: Isolating the methanol extract from M. elengi flowers and test its antibacterial and antifungal activity. Furthermore, extracts with active concentrations are made into a lotion. METHODS: Methanol extract from M. elengi flower was characterised by gas chromatography-mass spectrometry, then tested for antibacterial and antifungal, then made into the lotion. The lotion was tested again for its antimicrobial activity, physical and organoleptic properties. RESULTS: The most abundant chemical compounds in an extract of M. elengi based on characterisation with GC-MS is Borneol L; (Bicyclo [2.2.1] heptane-2-ol, 1,7,7-trimethyl, as much as 82%). The methanol extract of M. elengi flower can inhibit the growth of S. aureus bacteria and C. albicans fungi, and also the lotion from methanol extract can inhibit the growth of S. aureus bacteria, but the M. elengi flower extract lotion cannot inhibit the growth of C. albicans fungi. The lotion inhibits the growth of S. aureus bacteria, from a concentration of methanol extract of 8% and 16%. Lotion with 16% methanol extract has 81.33% in activity power compared with positive control. The results of physical and organoleptic properties test, with the concentration of methanol extract of M. elengi 1, 2, 8, and 16%, have pH in the range of 6.6-8 (still in a safe range, 4.5-9 according to SNI 16-4399-1997). The lotion type is m/a, the spreading capacity of the lotion is 18.8 - 39.5 cm2. The power of adhesive at skin ranges from 1 minute 27 seconds to 3 minutes 7 seconds. The viscosity of the lotion ranges from 23,670-24,400 cP, this range is in the range based on SNI 16-4399-1996 (2000-50,000 cP), so the lotion is in a good category. The preferred lotion is at a concentration of 2%, in fragrance. CONCLUSION: Antibacterial activity of the lotion of methanol extract of M. elengi flower against S. aureus bacteria was the best at 16%, but could not inhibit the growth of C. albicans fungi. The most abundant compounds in methanol extract are Borneol L compounds; (Bicyclo [2.2.1] heptane-2-ol, 1,7,7-trimethyl. In general, the physical properties of this lotion meet the requirements of SNI16-4399-1996, SNI 16-4399-1997, and lotions which are preferred at a concentration of 2% in fragrance.

scholarly journals FRAKSINASI EKSTRAK METANOL KULIT BATANG Rhizophora mucronata DAN UJI DAYA HAMBATNYA TERHADAP BAKTERI Escherichia coli

Molekul ◽  
2006 ◽  
Vol 1 (1) ◽  
pp. 30
Author(s):  
Dian Riana Ningsih ◽  
Warsinah Warsinah ◽  
Suwandri Suwandri
Keyword(s):  
Escherichia Coli ◽  
Infrared Spectroscopy ◽  
Methanol Extract ◽  
Chemical Compounds ◽  
Wide Distribution ◽  
Chemical Activity ◽  
Dilution Method ◽  
Rhizophora Mucronata ◽  
Mangrove Plant ◽  
E Coli

Rhizhophora mucronata is commonly found mangrove plant due to it’s wide distribution. The bark of this plant used for traditional medicine as slimming, antidiarrhea , anticonstipation. The chemical compounds of R. mucronata are tannins, alcaloide, flavanoids and saponin. In order to find out the effect of chemical activity of this plant, a research entitled Fractionation Metanol Extracts Bark of R. Mucronata and Inhibiting Capacity Assay againstEscherichia coli Bacteria has been carried out.The bark of R. mucronata was extracted by soxhlet with methanol solvent. Than methanol extract was fractionated with developing solvent kloroform : metanol (1:9) and producing five fractions that gave positive result on test for alcaloide compounds. The result of infrared spectroscopy that fraction 1-5 also contains of alcaloide. All fractions from coulomn chromatography tested on E. coli with dilution method which gave result that these fractions inhibit the growth of E. coli.

scholarly journals Ginkgo Biloba Leaf Extract Attenuates Atherosclerosis in Streptozotocin-Induced Diabetic ApoE-/- Mice by Inhibiting Endoplasmic Reticulum Stress via Restoration of Autophagy through the mTOR Signaling Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-19 ◽  
Author(s):  
Jinfan Tian ◽  
Mohammad Sharif Popal ◽  
Yanfei Liu ◽  
Rui Gao ◽  
Shuzheng Lyu ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Ginkgo Biloba ◽  
Leaf Extract ◽  
Chemical Compounds ◽  
Network Pharmacology ◽  
Diabetic Mice ◽  
Active Chemical ◽  
Ginkgo Biloba Leaf Extract ◽  
Ginkgo Biloba Leaf

Background. There is a crosstalk between endoplasmic reticulum stress (ERS) and autophagy, and autophagy could attenuate endoplasmic reticulum stress-mediated apoptosis. Ginkgo biloba leaf extract (GBE) exerts vascular protection functions. The purpose of the present study is to investigate the role of autophagy in diabetic atherosclerosis (AS) and the effect of GBE on autophagy and ERS. Methods. Network pharmacology was utilized to predict the targets and pathways of the active chemical compounds of Gingko biloba leaf to attenuate AS. ApoE-/- mice were rendered diabetic by intraperitoneal ingestion with streptozotocin combined with a high-fat diet. The diabetic mice were divided into five groups: model group, atorvastatin group, rapamycin group, and low- and high-dose GBE groups. Serum and tissue markers of autophagy or ERS markers, including the protein expression, were examined. Results. The mammalian target of rapamycin (mTOR) and NF-κB signaling pathways were targeted by the active chemical compounds of GBE to attenuate AS predicted by network pharmacology. GBE reduced the plaque area/lumen area and the plaque lipid deposition area/intimal area and inhibited the expressions of CD68, MMP2, and MMP9. Rapamycin and GBE inhibited the expression of mTOR and SQSTM1/p62 which increased in the aorta of diabetic mice. In addition, GBE reduced the expression of ERS markers in diabetic mice. GBE reduced the serum lipid metabolism levels, blood glucose, and inflammatory cytokines. Conclusion. Impaired autophagy and overactive endoplasmic reticulum stress contributed to diabetic atherosclerosis. mTOR inhibitor rapamycin and GBE attenuated diabetic atherosclerosis by inhibiting ERS via restoration of autophagy through inhibition of mTOR.

scholarly journals Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2422 ◽  
Author(s):  
Mohamad Fawzi Mahomoodally ◽  
Marie Carene Nancy Picot-Allain ◽  
Gokhan Zengin ◽  
Eulogio J. Llorent-Martínez ◽  
Hassan H. Abdullah ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Inhibitory Activity ◽  
Methanol Extract ◽  
Octadecenoic Acid ◽  
Antioxidant Potential ◽  
Bioactive Metabolites ◽  
Network Pharmacology ◽  
Therapeutic Benefits ◽  
Anacamptis Pyramidalis

Anacamptis pyramidalis (L.) Rich. forms part of the Orchidaceae family that is highly valued for its horticultural as well as therapeutic benefits. The present study set out to investigate the inhibitory activity of A. pyramidalis tubers against key biological targets for the management of type 2 diabetes, Alzheimer disease, and skin hyperpigmentation. In addition, the antioxidant potential of the extracts was also assessed using multiple methods. The detailed phytochemical profiles of the extracts were determined using high-performance liquid chromatography. Based on qualitative phytochemical fingerprint, a network pharmacology analysis was conducted as well. Parishin was identified from the water extract only, whereas gastrodin and caffeic acid derivatives were present in the methanol extract. The methanol extract exhibited high inhibitory activity against tyrosinase (69.69 mg kojic acid equivalent/g extract), α-amylase (15.76 mg acarbose equivalent/g extract), and α-glucosidase (20.07 mg acarbose equivalent/g extract). Similarly, the methanol extract showed highest antioxidant potential (22.12, 44.23, 45.56, and 29.38 mg Trolox equivalent/g extract, for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), CUPric Reducing Antioxidant Capacity (CUPRAC), and Ferric Reducing Antioxidant Power (FRAP) assays, respectively). Finally, the results of network pharmacology analysis, besides corroborating traditional uses of plant extracts in the management of cold and flu, confirmed a direct involvement of identified phytochemicals in the observed enzyme inhibitory effects, especially against tyrosinase, α-amylase, and α-glucosidase. Furthermore, based on the results of both colorimetric assays and network pharmacology analysis related to the activity of A. pyramidalis extracts and identified phytocompounds on enzymes involved in type 2 diabetes, a docking study was conducted in order to investigate the putative interactions of oxo-dihydroxy octadecenoic acid trihydroxy octadecenoic acid against aldose reductase, peroxisome proliferator-activated receptor (PPAR)-α, dipeptidyl peptidase (DPP)-IV, and α-glucosidase. Docking analysis suggested the inhibitory activity of these compounds against the aforementioned enzymes, with a better inhibitory profile shown by oxo-dihydroxy octadecenoic acid. Overall, the present findings supported the rationale for the use of A. pyramidalis as source of bioactive metabolites and highlight, today more than ever, for the strong necessity of linkage strategy between wild resource valorization and conservation policy.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAN SKRINING FITOKIMIA EKSTRAK HEKSANA, ETIL ASETAT DAN METANOL TUMBUHAN SURUHAN (Peperomia pellucida L. Kunth)

2020 ◽  
Vol 16 (2) ◽  
pp. 105-111
Author(s):  
Restianti Restianti ◽  
Bimo Budi Santoso ◽  
Maria Ludya Pulung
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test ◽  
Solvent Analysis

Antibacterial activity test and screening of phytochemical of extract hexane, ethyl acetate  and methanol have been carried out. The extraction of the P.pellucida plants was carried out by the maceration method in stages based on the polarity of the solvent. Analysis of chemical compounds done with phytochemical screening test. Based on the identification of chemical compounds by phytochemical screening, hexane and ethyl acetate extract has been detected only in the presence of flavonoid compounds with moderate categories. Methanol  extract contains high-grade alkaloids and moderate flavonoid and tannin compounds. Antibacterial test using disffusion methods suggest that ethyl acetate and methanol extract have categorically strong antibacterial activity againts the S. aureus  of 14 mm and 16 mm respectively. Hexane extract and acetate ethyl have a strong inhibition to  E. coli  of 11 mm and 12 mm respectively. Methanol extract has a weak inhibition to bacteria E. coli  and a hexane extract has a weak inhibition to bacteria S. aureus.

scholarly journals Identification of chemical compositions in some medicinal plants by GC/MS analysis

2019 ◽  
Vol 20 (1) ◽  
pp. 161-172
Author(s):  
S. Mohammadi ◽  
◽  
Kh. Piri ◽  
M. Dinarvand ◽  
◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Methanol Extract ◽  
Chemical Compounds ◽  
New Drugs ◽  
Gas Chromatography Mass Spectrometry ◽  
Vitamin B ◽  
Chemical Compositions ◽  
Potential Sources

Medicinal plantsused in treatment of diseases earlier times, are potential sources of new drugs. In this study, chemical compositions of Echium khuzestanicum, Marrubium anisodan and Echinops cephalotes were identified by Gas Chromatography-Mass Spectrometry(GC/MS).M.anisodanand E.cephalotes aerial organs and E.khuzistanicum flowers were extracted by maceration method. According to the results, 46 kinds of chemical compounds found in methanol extract of E.khuzestanicum. There are mucilage, fatty acids, flavonoid and diterpenes in the flower of E.khuzestanicum. Seventy sixkinds of chemical compounds were found in methanol extract of E.cephalotesincludingaldehydes (7.9%), phenols (7.5%), fatty acids (5.8%) and furfural (5.4%)and86 kinds of chemical compounds found in M.anisodan extract. Furfural, steroids, vitamin B and flavonoids are the main compounds of M.anisodan.

scholarly journals Test on the Antioxidant Activities of Methanol Extract of Bidara Leaves (Ziziphus spina-christi L.) using the DPPH Radical Immersion Method

2020 ◽  
Vol 3 (1) ◽  
pp. 44-51
Author(s):  
Dwi Bagus Pambudi ◽  
Nuniek Nizmah Fajriyah ◽  
Vidiah Rizka Shalekhah
Keyword(s):  
Free Radicals ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Chemical Compounds ◽  
Bioactive Compound ◽  
Flavonoid Compound ◽  
Immersion Method ◽  
Compound A ◽  
Ic50 Value ◽  
Layer Chromatography

Bidara (Ziziphus spina-christi L.) is a tropical tree originating from Sudan in which it is commonly known as "Nebeq" in Saudi Arabia. It is of a bioactive compound - a flavonoid compound, which is the potential to be used as an antioxidant. It is capable of inhibiting any cell damages caused by free radicals. This study aimed to measure the activities of the free radicals in methanol extracts of Z. spina-christi leaves. The process of extracting the Z. spina-christi leaves was carried out through the maceration method using methanol as a solvent. The qualitative analysis of chemical compounds with certain eluents using thin-layer chromatography (TLC) was purposely to determine the groups of active compounds in extracts. The measurement of antioxidant activities was carried out using the 1,1-Diphenyl-2-picryl Hidrazyl (DPPH) immersion method in which absorption was measured at a maximum wavelength of 513 nm. The results showed that the methanol extract of Z. spina-christi leaves had a very weak antioxidant activity with the IC50 value of 466.804 �g/ml. The results of the bioautographic profile showed the presence of flavonoid compounds, phenols, saponins, and tannins.

scholarly journals Unraveling the Molecular Mechanisms of Fructus Anisi Stellati as a Remedy for Infantile Colic by Network Pharmacology

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Xingyu Li ◽  
Yan Xu
Keyword(s):  
Molecular Mechanisms ◽  
Immunoglobulin A ◽  
Chemical Compounds ◽  
Network Pharmacology ◽  
Infantile Colic ◽  
Inflammatory Reactions ◽  
Pathway Enrichment ◽  
The Common ◽  
Iga Production ◽  
Therapeutic Compounds

Fructus anisi stellati (FAS) is an anise-scented star-shaped fruit from Illicium verum tree. It is commonly consumed in many cultures as food and medicine, particularly as a remedy for infantile colic (IC). The elucidation of molecular mechanisms of action would contribute to the understanding of the traditional therapy of FAS and help to guide the preclinical and clinical study of this herb. The aim is to investigate the key therapeutic compounds of FAS and to explore the underlying molecular mechanisms of FAS therapy. The chemical compounds of FAS were obtained through data mining on TCMSP and ADME screening, and the common targets of the FAS compounds and the IC-correlated diseases were obtained from PharmMapper, GeneCards, and OMIM databases. GO and KEGG databases were used for molecular function and pathway enrichment. Cytoscape was used for network construction and analysis. SystemsDock was used for molecular docking. Three key compounds (i.e., quercetin, luteolin, and kaempferol), 19 targets, 7 molecular pathways, and 12 IC-correlated diseases were identified to be involved in the molecular mechanisms of FAS for the treatment of IC. This work showed that three therapeutic modules were primarily engaged in the molecular mechanisms of FAS for IC therapy, including the inhibition of inflammatory reactions, stimulating immunoglobulin A (IgA) production in the gastrointestinal tract, and enhancing the secretion of digestive enzymes.

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