Relating tensile strength to acoustic and biological properties of sediments along a mud-sand gradient in Mobile Bay, AL

2021 ◽  
Vol 150 (4) ◽  
pp. A350-A350
Author(s):  
Kelly M. Dorgan ◽  
Grant Lockridge ◽  
Madeline R. Frey ◽  
W. Cyrus Clemo ◽  
Gabriel R. Venegas ◽  
...  
2018 ◽  
Vol 19 (11) ◽  
pp. 3433 ◽  
Author(s):  
Dorottya Kardos ◽  
István Hornyák ◽  
Melinda Simon ◽  
Adél Hinsenkamp ◽  
Bence Marschall ◽  
...  

Platelet-rich fibrin (PRF) membrane is a three-dimensional biodegradable biopolymer, which consists of platelet derived growth factors enhancing cell adhesion and proliferation. It is widely used in soft and hard tissue regeneration, however, there are unresolved problems with its clinical application. Its preparation needs open handling of the membranes, it degrades easily, and it has a low tensile strength which does not hold a suture blocking wider clinical applications of PRF. Our aim was to produce a sterile, suturable, reproducible PRF membrane suitable for surgical intervention. We compared the biological and mechanical properties of PRF membranes created by the classical glass-tube and those that were created in a single-syringe closed system (hypACT Inject), which allowed aseptic preparation. HypACT Inject device produces a PRF membrane with better handling characteristics without compromising biological properties. Freeze-thawing resulted in significantly higher tensile strength and higher cell adhesion at a lower degradation rate of the membranes. Mesenchymal stem cells seeded onto PRF membranes readily proliferated on the surface of fresh, but even better on freeze/thawed or freeze-dried membranes. These data show that PRF membranes can be made sterile, more uniform and significantly stronger which makes it possible to use them as suturable surgical membranes.


In this study, intelligent food packaging in the forms of film and coating were developed based on starch, chitosan and curcumin extracted from turmeric. Solution casting method was applied to develop the film. Both of the film and coating were evaluated and compared by their chemical, physical and biological properties. The film was evaluated in terms of tensile strength measurement, FTIR spectroscopy, antioxidant activity and antimicrobial activity as well as color difference parameters after application on the strawberry. The results obtained showed that the film has a tensile strength of 1.37 MPa, elongation at break of 18.9%, antioxidant activity of 95.65% and high antimicrobial activity as the film had successfully delayed the formation of mould on the strawberry after 5 days of storage. In addition, the stability of both film and coating were evaluated based on their applications on strawberries at two different conditions which are at room temperature and chiller temperature during 5 days storage to identify their potential use as intelligent food packaging. After 5 days, it was found that the film at room temperature had been partially degraded and the coating had caused colour degradation and texture deterioration of the strawberry. In contrast, the film and coating stored at chiller temperature remained the same in terms of physical structure and able to monitor and extend the shelf life of the strawberry. For the evaluation of the film as pH sensing film, the colour of the film changed after 5 days from 53.46 to 48.92 for L*, 26.01 to 22.68 for a* and 42.49 to 44.65 for b* at chiller temperature, while at room temperature, the values of L* changed from 53.96 to 48.96, 25.54 to 20.36 for a* and 46.34 to 44.10 for b*. These showed that the film was able to monitor the freshness of the strawberry by changing its colour in respond to pH changes of the strawberry. The results obtained revealed that both of the film and coating have a greater stability at chiller temperature as compared to storage at room temperature and both have a strong antioxidant activity and strong antimicrobial activity that they delayed the spoilage of the strawberries. Therefore, the film and coating based on starch, chitosan and curcumin can be used to monitor freshness of refrigerated food and have the potential to be used as intelligent food packaging


Author(s):  
Shivaji Kashte ◽  
Gajanan Arbade ◽  
R.K. Sharma ◽  
Sachin Kadam

In the bone tissue engineering composite scaffolds with osteogenic potential are emerging as the new tool. Here, we investigated the graphene (GP), graphene oxide (GO) andCissusquadrangularis(CQ) callus extract for their spontaneous osteoinductive potential. Electrospun poly ε-caprolactone (PCL) sheets were painted with varying combination GP, GO and CQ solutions as ink. The prepared PCL-GO, PCL-GO-CQ, PCL-GP and PCL-GP-CQ scaffolds were characterized for their physical, mechanical and biological properties. Addition of GO, GP, GO-CQ and GP-CQ to PCL enhanced roughness, wettability, Yield strength and tensile strength, biocompatibility .significantly. Presence of GO and CQ in PCL-GO-CQ scaffolds, while GP and CQ in PCL-GP-CQ scaffolds showed synergistic effect on the biocompatibility, Cell attachment,cell proliferation of human umbilical Wharton’s jelly derived mesenchymal stem cells (hUCMSCs) and their differentiation into osteoblasts by 21stday in culture without osteogenic differentiation media or any growth factors. Same is confirmed by the Alizarin red S staining and Von kossa staining. The combination of PCL-GO-CQ scaffold prepared by novel paint method was found to be the most potential in bone tissue engineering.


2018 ◽  
Vol 12 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Sina Khoshsima ◽  
Ammar Alshemary ◽  
Aysen Tezcaner ◽  
Sedat Surdem ◽  
Zafer Evis

In this study, hydroxyapatite-B2O3-La2O3 composites (with ? 20 wt.%B2O3 and ? 2 wt.% La2O3) were synthesized via wet precipitation method and calcined at 1100?C for 1 h. X-ray diffraction (XRD) analysis revealed the existence of the pure hydroxyapatite (HA) phase with high crystallinity. Characteristic absorption bands of HA were also observed in Fourier transform infrared spectra. Furthermore, scanning electron microscopy images demonstrated that the addition of B2O3 and La2O3 into HA enhanced the particle growth. Mechanical properties of the composites were studied by diametral tensile test and the results showed that incorporation of 10 wt.% B2O3 and 2 wt.% La2O3 led to a 39% increase in tensile strength (compared to the pure HA). In vitro cytocompatibility of HA-B2O3-La2O3 composites was investigated using Osteosarcoma Cell Lines (Saos-2). Incorporation of B2O3 and La2O3 into HA had no toxic effect towards the cells. Based on its tensile strength properties and biological response, composite of 88 wt.% HA, 10 wt.% B2O3 and 2 wt.% La2O3 was suggested as a promising composite for bone tissue engineering applications.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Narges Fereydouni ◽  
Jebrail Movaffagh ◽  
Nafise Amiri ◽  
Susan Darroudi ◽  
Aida Gholoobi ◽  
...  

AbstractCurcumin contains many biological activities as a natural bioactive substance, however, its low solubility stands as a huge bioavailability disadvantage. Recently, different methods have been developed for utilizing the tremendous medicinal properties of this material. In this study, an Oil/Water nano-emulsion of curcumin (Nano-CUR) has been woven in zein polymer at three percentages of 5%, 10%, and 15% (v/v). We have investigated the physicochemical properties of nanofibers (NFs) including FESEM, FTIR, tensile strength, encapsulation efficiency, and release profile, as well as biological properties. According to the data, the NFs have been observed to become significantly thinner and more uniformed as the involved percentage of Nano-CUR had been increased from 5 to 15%. It is considerable that the tensile strength can be increased by heightening the existing Nano-CUR from 5% towards 15%. The resultant NFs of zein/Nano-CUR 15% have exhibited higher in vitro release and lower encapsulation efficiency than the other evaluated zein/Nano-CUR NFs. It has been confirmed through the performed viability and antioxidant studies that zein/Nano-CUR 10% NFs are capable of providing the best conditions for cell proliferation. Considering the mentioned facts, this work has suggested that Nano-CUR can be successfully woven in zein NFs and maintain their biological properties.


2007 ◽  
Vol 330-332 ◽  
pp. 1425-1428 ◽  
Author(s):  
L.S. Ozyegin ◽  
R. Tuncer ◽  
E. Avci

Recasting of economic alloys can change several properties. The number of recasting was found to have negligible effect on surface texture and on the amount of corrosion products released. The methods and equipments utilized in the casting of an alloy are important on the quality of casting. Carbon incorporated in a noble or economic alloy during casting is known to affect the mechanical values of the metal. In the present study we aimed to investigate the change in structure and metal hardness due to recasting. Materials and method: The effect of recasting, up to four times of a non precious “Finalloy” commercial dental alloy on their Rocwell C hardness behavior and their microstructures was inspected. Using “Kerr casting waxes” patterns were prepared similar to the samples from “Finalloy”. The investments were pre-heated to phase at temperatures between 900°C and 1000°C. The alloy was melted by an acetylene-oxygen flame in a silicate crucible and four groups were compared: New alloy and alloys casted two, three, and four times (n = 7). After casting, Brinell harnesses were recorded. Values of Rocwell C strength, for each specimen group were analyzed by one-way analysis of variance, followed by the Student- Newman-Keuls multiple range test. A metal microscope was used to evaluate the surface morphology of the samples before hardness tests. 1st Casting :30.65 ± 0.3 kgf, 2nd Casting: 31.33 ± 0.4 kgf, 3rd Casting:34.80 ± 0.3 kgf, 4th Casting: 35.5 ± 28 kgf represents the Rocwell hardness of the castings. The results showed that hardness, increased with repeated castings. The experimental data indicates that increasing the number of successive recasting of “Finalloy” enhances the Rocwell C hardness, thus, after remelting and recasting, the biological properties decreases. Microscope study: Increase of the carbon content on the gren bounders of a cobalt-chromium alloy causes a considerable change in its microstructure. The hardness and yield point for 0.05 %, 0.1 % and 0.2 % tensile strength and elongation were determined for these test-pieces. The results showed that hardness, yield points and tensile strength increased with increased carbon content, whereas the elongation lessened. We determined that carbon concentrated at gren bounders. The clinical importance of the study is that recasting affected the properties of the metal, for best biological results with dental alloys always new metals must be used.


2018 ◽  
Vol 773 ◽  
pp. 354-359
Author(s):  
Lilis Kistriyani ◽  
Achmad Chafidz Mas Sahid ◽  
Tintin Mutiara ◽  
Dine Olisvia ◽  
Lutfa Rahmawati

Facial mask is an important part in every beauty treatment because it will give a smooth and gentle effect on the face. This research was done to make edible film that will be applied for face mask. The main ingredient in making this edible film was purple sweet potato powder with the addition of glycerol as plasticizer. One of the ingredients in purple sweet potato was a flavonoid compound. The purpose of this study was to determine the effect of glycerol concentration to flavonoids release, physical properties and biological properties of edible film produced. Two grams of the flour of purple sweet potato and one gram of carragenaan was dissolved in 100 mL of aquadest and were heated at 70°C. They were mixed with 0.3 gram K2SO4 in 50 mL of aquadest. Then, the glycerol were added which the variation of volume 1, 2, and 3 mL. The mixture was dried in the oven at 70°C for 20 hours. The analysis that had been done were spectrophotometer uv-vis analysis to find out how many flavonoids can be released into facial skin, tensile strength analysis and elongation of break analysis, biodegradability analysis, and microbiological analysis. The results of spectrophotometer uv-vis analysis showed that the most flavonoid release concentration was 20.33 ppm in the 2 mL glycerol variation. The best tensile strength value was 8,502 N and the greatest elongation of break value was 14% in 1 mL glycerol variation. In the biodegradability test, the more volume of glycerol added, the faster of the edible film was degraded. The results of microbiological analysis showed that the purple sweet potato extract had the ability to inhibit the growth of Propionibacterium acnes which was seen in the presence of inhibit zone was 18.9 mm.


Author(s):  
Tyler J. Allee ◽  
Wan-Ju Li

An emerging approach to repair and replace a great number of the ligament and tendon injuries that occur each year is the use of tissue engineering principles to fabricate replacement tissues. One of the current challenges in tendon/ligament tissue engineering is fabricating scaffolds that possess both the proper mechanical and biological properties. A promising strategy to produce such scaffolds is electrospinning. Electrospinning uses a high-voltage power supply to draw viscous polymer solutions into ultra-fine fibers with nanometer-scale diameters, thus structurally mimicking native extracellular matrix components, such as collagen fibrils. Nano-scale fibers have been previously shown to enhance cellular proliferation, as well as stimulate cells to maintain morphology and phenotype when compared to scaffolds composed of larger fibers [1]. Furthermore, electrospinning provides researchers the flexibility of fabricating aligned scaffolds with isotropic mechanical properties to mimic native tissue mechanics [2]. However, scaffolds composed of aligned electrospun nanofibers often do not possess the elasticity and tensile strength required to properly carry out the mechanical function of tendon/ligament tissues. One approach that has been used to enhance mechanical properties of fibrous scaffolds is to weave multiple fibrous bundles into a braided scaffold. [3]. In this study, we fabricated a novel tendon/ligament tissue engineering scaffold that combines enhanced biological properties of electrospun scaffolds with enhanced mechanical properties through braiding. Furthermore, our approach offers the versatility to tailor tensile strength and elasticity of the braided scaffolds simply by varying the pattern of braiding, making it possible for researchers to construct tendon/ligament scaffolds with various mechanical properties.


Author(s):  
U. E. Kurilova ◽  
A. Yu. Gerasimenko

The new method of the formation of nanocomposite materials based on carbon nanotubes for the regeneration of connective tissues has been developed.Aim. Study of the structure, mechanical characteristics and biocompatibility of the obtained materials.Materials and methods. The experimental samples of nanocomposite materials were based on multi-walled and singlewalled carbon nanotubes, the matrix was bovine serum albumin. A layer of liquid dispersion of the components on a silicon substrate or in a container was irradiated with laser radiation to form the solid nanocomposite material. The microstructure of the obtained samples was analyzed with X-ray microtomography, the tensile strength was investigated using a testing machine. Fibroblast cells were incubated with experimental samples for 3, 24, 48, and 72 h and then fixed with glutaraldehyde. Cell growth during incubation with samples was studied using optical and atomic force microscopy.Results. It was found that a slight decrease in tensile strength and increase in the degree of deformation were observed with an increase in the concentration of carbon nanotubes. At the same time, the mechanical parameters of the samples corresponded to the requirements for materials for the restoration of connective tissue defects. Microscopic studies indicate good adhesion of cells to the nanocomposite material, no toxic effect of the samples on the cells was found. After 3 hours of incubation, the cells had their original rounded shape, after 24 hours of incubation cells began to proliferate on the sample’s surface and were spindle-shaped. After 48 and 72 hours, the cells practically formed a monolayer on the surface of the samples.Conclusion. The results of the study show that the structural and mechanical parameters of the developed nanocomposite materials meet the requirements of biomedicine. It was also shown that nanocomposite materials do not suppress cell growth and can serve as a scaffold for the regeneration of damaged tissues.


2019 ◽  
pp. 152808371986744
Author(s):  
Misbah ◽  
Ijaz Ahmad Bhatti ◽  
Khalid Mahmood Zia ◽  
Haq Nawaz Bhatti ◽  
Muhammad Shahid

In the present project sodium alginate-based polyurethane dispersions were synthesized by two shot processes, using isophorone diisocyanate, polyethylene glycol (Mn-300), dimethylol propionic acid as internal emulsifier along with other reagents including triethylamine and dibutyltindilaurate catalyst. Molecular characterization was performed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. Physical properties were observed and samples were found to be translucent yellow with a stability of more than one year. Biological properties such as blood hemolytic and antibacterial action were also noted in order to check if the samples can be used inside human body for bandage coatings. Synthesized dispersions were found to have considerable blood hemolytic activity and good antibacterial activity. After the complete characterization, dispersions were applied on polycotton blend fabric (50/50). After the treatment, fabric was analyzed for its tear strength, tensile strength pilling resistance and morphological properties by scanning electron microscopy. Fabric treated with polyurethane dispersions has decreased tear strength, enhanced tensile strength, improved pilling resistance and more intact appearance as compared to the untreated fabric.


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