Effect of hydrogen peroxide on the sustained release of 99mTc-labeled cytarabine in melanoma of C57 mice by ECT imaging pharmacology.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e15569-e15569
Author(s):  
Yan Han ◽  
Qiang Fu ◽  
Baofa Yu ◽  
Keyword(s):  
Hydrogen Peroxide ◽  
Treatment Group ◽  
Sustained Release ◽  
Residence Time ◽  
Tumor Model ◽  
Intratumoral Injection ◽  
Control Group ◽  
Release Agent ◽  
Mouse Tumors ◽  
Long Time

e15569 Background: To investigate the residence time of 99mTc-labeled cytarabine in mouse tumors under ECT imaging. Methods: The subcutaneous solid tumor model of mice was established by injection of melanoma cell suspension into the left forelimb of C57 mice. The tumor-bearing mice were divided into treatment group and control group when the longest diameter of the tumor was 5-8 mm. Group 3 only. The mice in the control group were given intratumoral injection of 0.1 ml of 99mTc-labeled cytarabine solution, and the mice in the treatment group were given intratumoral injection 0.1 ml 99mTc labeled cytarabine combined with hydrogen peroxide solution at 0.25 h, 0.5 h, 1 h. After 2 hours and 19 hours of administration, the radioactivity intensity values were observed by SPECT Apx-409 ECT phenomenon equipment. Results: The T/W values of ECT imaging in 3 mice in the treatment group were higher than those in the control group at 0.25 h, 0.5 h, 1 h, 2 h and 19 h. The T/W values of ECT imaging decreased in the treatment group at different time points. The amplitude was lower, and the T/W values in each period were higher than those in the control group. Conclusions: The sustained release agent hydrogen peroxide can significantly increase the residence time of 99mTc-labeled cytarabine in the tumor. It is speculated that hydrogen peroxide has a positive effect on prolonging the drug action time of cytarabine and improving the effect of killing tumor cells. It showed that H2O2 can hold the Ara-C for a long time in tumor.

scholarly journals PENGOLAHAN SAMPAH SAYURAN MENJADI KOMPOS DENGAN METODE TAKAKURA

IKESMA ◽  
2019 ◽  
pp. 81
Author(s):  
Adella Atika Larasati ◽  
Septa Indra Puspikawati
Keyword(s):  
Treatment Group ◽  
Waste Management ◽  
Environmental Health ◽  
Research Method ◽  
Control Group ◽  
Physical Parameters ◽  
Vegetable Waste ◽  
Physical Quality ◽  
Long Time ◽  
Temperature Parameter

The increasing household activity is increasing also the amount of  vegetables waste produced will cause a pile of decomposed waste, so that proper waste management is needed. The processing of waste done by the Community is still conventional that takes a long time so needed an innovation by re-utilizing garbage into compost with the method Takakura. This research aims to determine the comparison of physical parameters (temperature, pH, humidity, smell, color and texture) between the control group and the treatment group in making compost with Takakura method. This research method utilizes an experimental semi-quasi design with a control group and a treatment group conducted in the Environmental Health Laboratory of  PSDKU Airlangga University in Banyuwangi during February-April 2019. The results showed that the parameters of physical quality compost vegetable waste without the addition of bioaktivator is the temperature of 29oC, pH 6.8, humidity 45%, colored blackish, smelling soil and texture like soil. While the parameters of physical quality compost vegetable garbage with the addition of Bioaktivator EM4 is 30oC temperature, pH 6.8, humidity 50%, colored blackish, smelling soil and texture like soil. Conclusion of making compost with vegetable garbage method Takakura obtained the temperature parameter of the treatment group higher than the control group, the pH of both groups, the humidity of the treatment group is higher or moist than With the control group as well as the color, smell and texture of both groups in accordance with the guidelines SNI 19-7030-2004. Keywords: compost, EM4, vegetable waste, takakura

scholarly journals The Using Piper ornatum as Biopesticide Larvae Musca domestica

BIOEDUSCIENCE ◽  
2020 ◽  
Vol 4 (1) ◽  
pp. 106-112
Author(s):  
Anita Dewi Moelyaningrum ◽  
Violita Pita Nugraheni ◽  
Prehatin Trirahayu Ningrum
Keyword(s):  
Treatment Group ◽  
Essential Oils ◽  
Experimental Method ◽  
Musca Domestica ◽  
House Fly ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Long Time

Background: Red betel (Piper ornatum) contains several compounds including flavonoids, alkaloids, tannins, and essential oils that have the ability as bioinsecticides. The purpose of this study was to analyze the differences in the number of deaths of house fly larvae (Musca domestica) exposed to red betel in the control group (0%) and the treatment group with a concentration of 1%; 1.5% and 2% for 24 hours. Methods: used is true experimental method with only posttes control group design. There were 4 treatments with 6 replications per treatment. Each treatment was described on 8 larvae, so the number of larvae used in this study was 192 tails. Results: Research shows that red betel powder is indeed effective in killing Musca domestica larvae, but it still requires a long time, which is at least 24 hours. Concentration is needed at least 2% if used as a biopesticide to reduce the density of flies. Conclusion: There was no significant difference in the mortality of Musca domestica larvae in the administration of red betel powder (Piper ornatum.  

scholarly journals MODIFIED METHOD FOR PURULONECROTIC FOOT FLEGMON TREATMENT

2020 ◽  
pp. 80-89
Author(s):  
E.Kh. Akramov ◽  
A.A. Beysembaev ◽  
V.Kh. Gabitov ◽  
S.K. Sulaymankulova ◽  
Omurbek uulu Ulukmyrza ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Treatment Group ◽  
Limb Salvage ◽  
Active Treatment ◽  
Active Treatment Group ◽  
Limb Amputation ◽  
Control Group ◽  
Kyrgyz Republic ◽  
Scientific Center ◽  
Modified Method

Every 30 seconds somewhere in the world there occurs a lower limb amputation in a patient with foot phlegmon. Antibacterial agents are the basis of systemic pharmacotherapy of surgical infections. However, such treatment is rather difficult as it is associated with the loss of antibiotic sensitivity. The purpose of the study is to determine the antibacterial effects of innovative hydrogen peroxide and nanosilver composition combined with chitosan lymphostimulation in foot phlegmon treatment for limb salvage. Materials and Methods. The work is based on the analysis of 46 patients’ treatment outcomes. All the patients suffered from purulent-necrotic foot phlegmons and were treated at the Scientific Center for Reconstructive Surgery, the Ministry of Health of the Kyrgyz Republic and Chuy Regional Combined Hospital. The patients were divided into 2 groups: control group (n=22) – conventional treatments, and active treatment group (n=24) – additional administration of bactericidal-lymphotropic cocktail. The groups were comparable in sex, age, and soft tissue involvement. Results. In patients of active treatment group wound cleansing from wound detritus was observed in 5 days, while in patients undergoing conventional treatment it was detected only in 9 days. In the active treatment group, the granulation time was 1.8 times faster, and the normalization of the leukocyte intoxication index was 2.5 times earlier. The dynamics of the level of medium-weight molecules indicated that lymphostimulation accelerated the interstitium detoxification process in the affected limb by 3 times. Conclusion. The proposed modified method for foot phlegmon treatment with bactericidal-lymphostimulating technologies accelerates tissue regeneration and contributes to limb salvage. Keywords: foot phlegmon, hydrogen peroxide, nanosilver, chitosan. Каждые 30 с в мире пациенту с флегмоной стопы производится ампутация нижней конечности. Основу системной фармакотерапии хирургических инфекций составляют антибактериальные средства, но лечение представляет существенные трудности, связанные с потерей чувствительности к антибиотикам. Цель исследования. Определить эффективность применения инновационной антибактериальной композиции перекиси водорода с наносеребром в комплексе с лимфостимуляцией хитозаном при лечении флегмон стопы в целях сохранения конечности. Материалы и методы. Работа основана на анализе результатов лечения 46 больных с гнойно-некротическими флегмонами стопы, находившихся на лечении в Научном центре реконструктивно-восстановительной хирургии МЗ КР и Чуйской областной объединенной больнице. Все больные, разделенные на 2 группы (контрольная (n=22) – традиционное лечение; основная (n=24) – дополнительное применение бактерицидно-лимфотропного коктейля), были сопоставимы по характеру поражения мягких тканей, полу и возрасту. Результаты. Очищение ран от раневого детрита в основной группе наступало через 5 сут, тогда как при традиционном лечении – только через 9 дней. Появление грануляций в основной группе происходило в 1,8 раза быстрее, нормализация лейкоцитарного индекса интоксикации – в 2,5 раза раньше. Динамика уровня молекул средней массы свидетельствовала о том, что лимфостимуляция ускоряет процесс детоксикации интерстиция региона пораженной конечности в 3 раза. Выводы. Применение предложенного модифицированного способа при лечении флегмон стопы с использованием бактерицидно-лимфостимулирующих технологий ускоряет регенерацию региона и создает условия для сохранения конечности. Ключевые слова: флегмона стопы, перекись водорода, наносеребро, хитозан.

Immunological functional study by use of the chemotherapy drug plus immunologic hapten like penicillin on the fibrosis of local tumor inflammatory reaction and the immunity of tumor-bearing mice.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e15196-e15196
Author(s):  
Bian Li ◽  
Dong Chen ◽  
Yan Han ◽  
Jian Zhang ◽  
Guoqin Zheng ◽  
...  
Keyword(s):  
Inflammatory Reaction ◽  
Solid Tumor ◽  
Tumor Tissue ◽  
Tumor Model ◽  
Intratumoral Injection ◽  
Control Group ◽  
Group V ◽  
Tumor Bearing ◽  
Group I ◽  
Solid Tumor Model

e15196 Background: Observe the antitumor effect of intratumoral injection of chemotherapy drugs or combined with immune adjuvant on Balb/c and c57 tumor bearing mice and the local inflammatory response, fibrosis and immune effect of tumor. Methods: H22 ascites tumor was inoculated in the armpit of BABL / c mice left forelimb to establish subcutaneous solid tumor model of liver cancer, B16 melanoma tumor filtrate was inoculated in the armpit of C57 mice left forelimb to establish subcutaneous solid tumor model of melanoma. The mice were randomly divided into DNP control group, penicillin control group, I group, II group, III group, IV group, V group, VI group, DOX control group, PYM control group, BLM control group and control group when the longest tumor diameter was about 5-8mm. On the first day of the experiment, the mice in each group were injected with corresponding of different group drugs. Three mice in each group were killed on day 1, day 4 and day 7 respectively and then the tumors were taken The C57 mice were used for immunohistochemistry BABL/c mice to detect the content changes of various fibroproliferative, inflammatory and immune related factors. Results: I group: The expression of COL1A1, CD4, IL12A, TGFB1, elastin, NFkB, COX 2, CD11c, CD8 and TNFa in group I was higher than that in other factors at day 1. After 4 and 7 days of administration, all factors decreased significantly; The results of C57 Mouse immunohistochemistry showed that the expression of CD4, cd11bc, Foxp3 in tumor tissue of group V was significantly increased on the first day after administration, and the expression of CD4, CD8, CD86, CD11bc in tumor tissue of group V was significantly increased on the seventh day. Conclusions: The results of immunohistochemistry and PCR showed that the content of fibroproliferative, inflammatory and immune factors in tumor tissue changed significantly after administration og intratumoral injection of combination drugs and penicillin with H2O2. All the above results has showed that chemotherapy drug and hapten was released in the tumor through H2O2 and induced the inflammatory reaction inside tumor (including various fiber proliferation and tumor growth inhibition) as well as the systemic immune response, this also is called abscopal effect.

Abscopal effect induced intratumoral injection of chemotherapy drugs with hapten and tumor microstructure changes with protein expression of distal untreated tumors in mice.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e15518-e15518
Author(s):  
Baofa Yu ◽  
Feng Gao ◽  
Peng Jing ◽  
Jian Liu ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Sustained Release ◽  
Negative Control Group ◽  
Intratumoral Injection ◽  
Negative Control ◽  
Drug Group ◽  
Control Group ◽  
Abscopal Effect ◽  
Chemotherapy Drugs ◽  
Release Drug ◽  
The Difference

e15518 Background: To observe the abscopal effect by intratumoral injection of chemo drugs with hapten on tumor, and analyze the difference in expression of bilateral tumor proteins and the inhibition of tumor growth on the opposite side. Methods: Balb/c mice 45, H22 ascites were inoculated in left forelimb armpit. When longest diameter of about 4-5mm randomized (day 0); the next day, intratumoral injection different combination drugs, the three days later in the right armpit mice were inoculated inoculated 0.2ml (105/ml) H22 cells, the seven days later of secondary administration, while observing the contralateral sides, especially tumor growth. 15 days after first treatment each group were sacrificed 6 mice, tumor tissue on both sides for immunohistochemistry, quantitative RNA PCR quantitative determination. Results: It showed an upward trend in body weight in mice, 15 days after administration of DNP sustained release drug group and PYM control group of mice were significantly lower than the body weight of the negative control group, the difference between groups (P<0.05); three groups of mice after administration of tumor volume on the left. On the 15th day after the administration, the left and right tumors in DNP group were significantly smaller than those in PYM group and negative control group, and the difference was statistically significant (P < 0.05); the left tumors in PYM group were significantly smaller than those in negative control group, and the difference was statistically significant (P < 0.05). After administration PYM control group’s Col1a1, CD4, Il12aA, TGF, Ela, NFKB, Cox2, CD11c, CD8, TNFa expression was significantly lower than the negative control group and DNP sustained-release drug group; DNP sustained-release drug group’s Col1a1, CD4,Il12aA, TGF, Elastin, NFKB, CD11c, CD8 expression was significantly lower than the negative control group. A large number of cell disintegration phenomenon, observed lymphocytes, leukocytes, the presence of high electron density granular, suggesting that there may be drugs enter into cancer cells. Conclusions: Intratumoral injection of chemotherapy drugs with hapten can exhibit significant anti-tumor effect. In the DNP sustained-release drug group, abscopal effect is more obvious. After the use of PCR quantitative detection of various tumors and fibroplasia, inflammatory factors and immune-related factors have changed significantly to further confirm abscopal effect by intratumoral injection of combination drugs.

scholarly journals Clinical Observation on the Treatment of Hypertension by Combination of Modified Banxia Baizhu Tianma Decoction and Auricular Acupuncture

2021 ◽  
Vol 5 (3) ◽  
Author(s):  
Renjie Zhou ◽  
Liping Yang ◽  
Hongxing Zhang ◽  
Wangbo Cao
Keyword(s):  
Quality Of Life ◽  
Blood Pressure ◽  
Treatment Group ◽  
Sustained Release ◽  
Western Medicine ◽  
Auricular Acupuncture ◽  
Control Group ◽  
Sustained Release Tablets ◽  
Better Than

Objective: To observe the curative effect of Jiawei Banxia Baizhu Tianma Decoction (BBTD) in combination with auricular acupuncture in the treatment of primary hypertension with phlegm-dampness syndrome. Methods: 120 patients with hypertension were randomly divided into a pure Western medicine treatment control group (referred to as the control group) and an integrated traditional Chinese and Western medicine treatment group (referred to as the treatment group), with 60 cases in each group. The control group was given nifedipine sustained-release tablets orally; the treatment group: nifedipine sustained-release tablets were given on the basis of modified BBTD combined with auricular acupuncture. The treatment course duration of the two groups was 4 weeks. Before and after treatment, the two groups were evaluated for treatment efficacy and quality of life. Results: There is statistically significant difference in blood pressure lowering between the treatment group and the control group (P?0.05); the treatment group was better than the control group in symptoms improvement of the patients (P?0.05); in terms of the improvement in quality of life , the treatment group was significantly better than the control group (P?0.05, P?0.01). Conclusion: Modified BBTD combined with auricular acupuncture method can reduce blood pressure and improve clinical symptoms in patients with primary hypertension with phlegm-dampness syndrome, and significantly improve the quality of life of patients.

Gum arabic helps prepare better lacey polymer films for specimen support in electron microscopy

1993 ◽  
Vol 51 ◽  
pp. 240-241
Author(s):  
Shailesh R. Sheth ◽  
Jayesh R. Bellare
Keyword(s):  
Electron Microscopy ◽  
Polymer Films ◽  
Gum Arabic ◽  
Complete Removal ◽  
Ammonium Bromide ◽  
Release Agent ◽  
Astigmatism Correction ◽  
Long Time ◽  
Micro Holes ◽  
Cetyl Trimethyl Ammonium

Specimen support and astigmatism correction in Electron Microscopy are at least two areas in which lacey polymer films find extensive applications. Although their preparation has been studied for a very long time, present techniques still suffer from incomplete release of the film from its substrate and presence of a large number of pseudo holes in the film. Our method ensures complete removal of the entire lacey film from the substrate and fewer pseudo holes by pre-treating the substrate with Gum Arabic, which acts as a film release agent.The method is based on the classical condensation technique for preparing lacey films which is essentially deposition of minute water or ice droplets on the substrate and laying the polymer film over it, so that micro holes are formed corresponding to the droplets. A microscope glass slide (the substrate) is immersed in 2.0% (w/v) aq. CTAB (cetyl trimethyl ammonium bromide)-0.22% (w/v) aq.

A Newspaper’s Effect on the Strength of Automatic Associations in Memory

2012 ◽  
Vol 24 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Florian Arendt
Keyword(s):  
Treatment Group ◽  
Term Effect ◽  
Control Group ◽  
Implicit Measure ◽  
Short Term ◽  
Short Term Treatment ◽  
Short Term Effect ◽  
Automatic Associations ◽  
Foreign Nationality ◽  
The Impact

A test was done to see if reading a newspaper which consistently overrepresents foreigners as criminals strengthens the automatic association between foreign country and criminal in memory (i.e., implicit cultivation). Further, an investigation was done to find out if reading articles from the same newspaper produces a short-term effect on the same measure and if (1) emotionalization of the newspaper texts, (2) emotional reactions of the reader (indicated by arousal), and (3) attributed text credibility moderate the short-term treatment effect. Eighty-five participants were assigned to one of three experimental conditions. Participants in the control group received short factual crime texts, where the nationality of the offender was not mentioned. Participants in the factual treatment group received the same texts, but the foreign nationality was mentioned. Participants in the emotionalized treatment group received emotionalized articles (i.e., texts which are high in vividness and frequency) covering the same crimes, with the foreign nationality mentioned. Supporting empirical evidence for implicit cultivation and a short-term effect was found. However, only emotionalized articles produced a short-term effect on the strength of the automatic association, indicating that newspaper texts must have a minimum of stimulus intensity to overcome an effect threshold. There were no moderating effects of arousal or credibility pertaining to the impact on the implicit measure. However, credibility moderated the short-term effect on a first-order judgment (i.e., estimated frequency of foreigners of all criminals). This indicates that a newspaper’s effect on the strength of automatic associations is relatively independent from processes of propositional reasoning.

Perbandingan Efek Suplementasi Tablet Tambah Darah Dengan Dan Tanpa Vitamin C Terhadap Kadar Hemoglobin Pada Ibu Hamil Dengan Usiakehamilan 16-32 Minggu Di Desa Keniten Kecamatan Mojo Kabupaten Kediri

2017 ◽  
Vol 3 (1) ◽  
pp. 76
Author(s):  
Siti Asiyah ◽  
Dwi Estuning Rahayu ◽  
Wiranti Dwi Novita Isnaeni
Keyword(s):  
Treatment Group ◽  
Vitamin C ◽  
Gestational Age ◽  
Group Treatment ◽  
Sampling Technique ◽  
Hemoglobin Level ◽  
The Body ◽  
Control Group ◽  
Fetal Organ ◽  
And Control

The needed of Iron Tablet in pregnancy was increase than mother who not pregnant.  That  cause of  high metabolism at the pregnancy for formed of  fetal organ and energy. One of effort for prevent anemia in mother pregnant with giving the Iron tablet and vitamin c. The reason of  this research in 4 June – 11 July 2014 is for compare the effect of  iron tablet suplementation with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks In Desa Keniten Kecamatan Mojo Kabupaten Kediri. This research method using comparative analytical.  Research design type of Quasy Eksperiment that have treatment group and control group. Treatment group will giving by Iron tablet and 100 mg vitamin C, and control group just giving by iron tablet during 21 days. Population in this research are all of mother pregnant with Gestational Age Of 16-32 Weeks with Sampling technique is  cluster random sampling is 29 mother pregnant. Comparison analysis of  iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks, data analysis using Mann Whitney U-test and the calculated U value (44,5) less than U-table (51). So there was difference of iron tablet suplementation effect with and without vitamin C toward Hemoglobin level in mother pregnant With Gestational Age Of 16-32 Weeks Therefore, the addition of vitamin C on iron intake is needed to increase the uptake of iron tablets. When the amount of iron uptake increases, the reserves of iron in the body will also increase, so as to prevent anemia in pregnant women; Keywords : Iron Tablet (Fe), Vitamin C, Hemoglobin level, Mother Pregnant

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

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