scholarly journals Antimicrobial Properties of Acrylic Resin Incorporated with Propolis Nanoparticles

2021 ◽  
Author(s):  
Sepideh Arab ◽  
Abbas Bahador ◽  
Ahmad Sodagar ◽  
Maryam Pourhajibagher ◽  
Azam Akhavan ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Acrylic Resin ◽  
Control Group ◽  
Biofilm Inhibition ◽  
Microbial Inhibition ◽  
Component Test ◽  
Inhibition Zones ◽  
Microbial Growth Inhibition ◽  
Removable Appliances

Objectives: One of the main problems with polymethyl methacrylate (PMMA) used for the fabrication of oral removable appliances is plaque accumulation due to surface porosities. Incorporation of antimicrobial agents in this material might help tackle this problem. The aim of this study was to evaluate the antimicrobial activity of PMMA acrylic resin incorporated with propolis nanoparticles (PNPs). Materials and Methods: Antimicrobial properties of acrylic resin incorporated with PNPs were assessed against Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), Lactobacillus acidophilus (L. acidophilus) and Candida albicans (C. albicans). Acrylic discs were fabricated in four groups: A control group without PNPs and three experimental groups containing 0.5%, 1% and 2% concentrations of PNPs. Disc agar diffusion (DAD) test was performed to determine the antimicrobial effects of PNPs by measuring the microbial growth inhibition zones on Muller-Hinton agar plates. The eluted components test evaluated the viable counts of microorganisms in liquid medium after 24 and 72h. Finally, biofilm inhibition test assessed the efficacy of PNPs for inhibition of biofilm formation. P<0.05 was considered significant.  Results: The acrylic discs failed to produce microbial inhibition zones in the DAD test. Discs containing 1% and 2% nanoparticles showed anti-biofilm effects on all four microbial species. The colony counts of all microorganisms significantly decreased following exposure to liquids containing nanoparticles after 24 and 72h in eluted component test. Conclusion: PMMA acrylic discs incorporated with PNPs presented some antimicrobial properties against S. mutans, S. sanguinis, L. acidophilus, and C. albicans.

scholarly journals Antimicrobial and mechanical acrylic resin properties with silver particles obtained from Fusarium oxysporum

2018 ◽  
Vol 21 (1) ◽  
pp. 96 ◽  
Author(s):  
Tabata Prado Sato ◽  
Celio Ilidio Conjo ◽  
Rodnei Dennis Rossoni ◽  
Juliana Campos Junqueira ◽  
Renata Marques de Melo ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Fusarium Oxysporum ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Acrylic Resin ◽  
Control Group ◽  
Significant Difference ◽  
Color Alteration ◽  
Cie Lab

<p><strong>Objective: </strong>This study evaluated the effects of the incorporation of silver nanoparticles (AgNPs) obtained from <em>Fusarium oxysporum</em> on heat-activated acrylic resin (HAAR) and their influence on resin’s surface roughness, hardness, color alteration and antimicrobial capacity against <em>Candida albicans</em>. <strong>Material and Methods:</strong> For this, 50 discs of HAAR (2x5 mm) were produced and divided into three groups, Control: HAAR; Ag1: HAAR plus 0.539 mg of AgNPs; and Ag2: HAAR plus 1.1 mg of AgNPs. Knopp hardness (HK), surface roughness (Ra and Rz) and color alteration according to the CIE Lab were measured. Specimens were then evaluated <em>in vitro</em> with regard to <em>C. albicans</em> biofilm formation through formed colony count (CFU/mL). Scanning Electron Microscopy (SEM) and Atomic force microscopy (AFM) analyses were performed. <strong>Results:<em> </em></strong>The addition of AgNPs of both concentrations changed Ra, Rz and HK significantly. There was statistically significant difference for L (p=0.00); a*(p=0.00) and b*(p=0.00) parameters. There were no differences between Ag1 and Ag2 biofilm formation, but the comparison of both with the control group presented a significant reduction (p=0.0091) on biofilm formation. SEM and AFM images showed no signs of NPs clustering. <strong>Conclusion:</strong> It can be concluded tha AgNPs incorporation in HAAR was effective in reducing <em>C. albicans</em> activity, with a slight change in color and hardness of the material, being effective therefore, in regions such as the dental prostheses palate, which have lesser aesthetic appeal.</p><p><strong>Keywords</strong></p><p>Acrylic resins; Silver; Antimicrobial agents.</p>

Anti-microbial Efficacy of Commercially Available Herbal Dentifrices on Streptococcus mutans and Candida albicans

2020 ◽  
pp. 87-94
Author(s):  
Naziya K. Babu ◽  
I. Meignana Arumugham ◽  
R. Pradeep Kumar ◽  
D. Sri Sakthi ◽  
S. Shiva Shanmugham
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Zone Of Inhibition ◽  
Oral Microflora ◽  
Naturally Occurring ◽  
Inhibition Zones ◽  
Susceptibility Tests ◽  
Almost All

Dental caries is a multi-factorial disease .Various chemically derived antimicrobial agents are incorporated to dentifrices to curb cariogenic microorganisms. There has been renewed interest in naturally occurring products in recent years, the side effects encountered with the use of synthetic formulations led to the surge for novel alternatives. To compare the efficacy of herbal dentifrices on oral microflora using antibiotic susceptibility tests. Two strains of microorganisms- Streptococcus mutans, and Candida albicans were taken and incubated in Mutans media, Sabouraud Dextrose agar respectively. Different dilutions (1:5, 1:10, 1:15) of several brands of toothpastes with different compositions were made. Sterile discs were incorporated with equal amounts of prepared toothpaste formulations. Then these discs were placed equidistant to each other followed by plates were incubated for 24 hours. Zone of inhibition is found to be higher in Ayush (30 mm), followed by Dant kanti, Colgate vedshakti, Meswak, Dantajeevan and Dabur red showing inhibition zones of 26 mm, 24 mm, 17 mm and 14 mm respectively. Results show that herbal toothpaste formulations were effective in controlling the oral microflora. The antimicrobial properties of the six dentifrices concluded that almost all the commercially available herbal dentifrices had antimicrobial activity to some extent.

scholarly journals Effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin: in vivo study in rats

2006 ◽  
Vol 14 (5) ◽  
pp. 319-323 ◽  
Author(s):  
Thales Rocha Mattos Filho ◽  
Marcelo de Souza Junqueira ◽  
Francisco Carlos Groppo ◽  
Rogério Heládio Lopes Motta ◽  
Fábio Ferreira Perazzo
Keyword(s):  
Antimicrobial Agents ◽  
Tissue Concentration ◽  
Diclofenac Sodium ◽  
Serum Levels ◽  
Control Group ◽  
Cervical Plexus ◽  
Inflammatory Drugs ◽  
Inhibition Zones ◽  
Group 1

OBJECTIVE: Antimicrobial agents in combination with anti-inflammatory drugs have been usually prescribed in both Medicine and Dentistry. However, few scientific reports support this clinical practice. The aim of this study was to evaluate the effect of betamethasone and diclofenac sodium on serum and tissue concentration of amoxicillin in rats. METHODS: Four polyurethane sponges were implanted in the back skin of 48 rats. After seven days the animals were divided into 6 groups (n=8). Group 1: amoxicillin (25 mg/kg); G2: diclofenac sodium (2.5 mg/kg); G3: betamethasone (0.1 mg/kg); G4: diclofenac sodium and amoxicillin; G5: betamethasone and amoxicillin; and G6: 0.9% sodium chloride solution (1.0 mL - control group). All drugs were administered in a single dose. After 90 minutes, the granulomatous tissues of each animal were surgically removed and weighed. Blood was collected from cervical plexus, centrifuged and 10µL of serum was placed on paper discs. In order to estimate amoxicillin concentration, serum and granulomatous tissues were separately submitted to microbiological assay, which used 10(8)cfu/mL of Staphylococcus aureus ATCC 25923 (penicillin-susceptible strain). After incubation (18 hours, 37ºC) the inhibition zones were measured and compared to a regression curve. RESULTS: No inhibition zones were observed for groups 2, 3 and 6. Tissue and serum concentrations of both G1 (4.14µg/g and 2.06µg/mL, respectively) and G5 (3.87µg/g and 1.70µg/mL, respectively) showed statistically significant differences (Kruskal-Wallis, p<0.05) in comparison to G4 (1.45µg/g and 0.41µg/mL, respectively). G1 and G5 did not differ significantly (p>0.05). CONCLUSION: Considering single doses, betamethasone did not interfere with amoxicillin levels but diclofenac sodium reduced both tissue and serum levels of amoxicillin in rats.

Chemical Composition and Antimicrobial Activity of the Essential Oils from Chloranthus japonicus Sieb. and Chloranthus multistachys Pei

2010 ◽  
Vol 65 (11-12) ◽  
pp. 660-666 ◽  
Author(s):  
Jie-fang Kang ◽  
Yuan Zhang ◽  
Yong-liang Du ◽  
Zhe-zhi Wang
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Disc Diffusion ◽  
Natural Sources ◽  
Pharmaceutical Industries ◽  
Inhibition Zones ◽  
Microbroth Dilution

We examined the composition and antimicrobial activity of two essential oils from Chloranthus japonicus Sieb. and Chloranthus multistachys Pei. GC-FID and GC-MS analyses identifi ed 48 and 39 compounds, which represented 95.56% and 94.58%, respectively, of all components in these oils. Of these, 28 compounds were common to both, with a relatively high amount of oxygenated monoterpenes (50.95% and 39.97%). Antimicrobial properties were evaluated in vitro via disc diffusion and microbroth dilution assays. Activities were strong against most tested microorganisms, with inhibition zones ranging from 8.1 to 22.2 mm. For both species, minimum values for inhibitory and bactericidal concentrations were 0.39 to 12.50 mg/mL and 0.78 to 50.00 mg/mL, respectively. These results suggest that these essential oils are potent natural sources of antimicrobial agents for the medicinal and pharmaceutical industries

Antimicrobial properties of actively purified secondary metabolites isolated from different marine organisms

2020 ◽  
Vol 21 ◽  
Author(s):  
Nilushi Indika Bamunu Arachchige ◽  
Fazlurrahman Khan ◽  
Young-Mog Kim
Keyword(s):  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Cost Effective ◽  
Resistance Mechanisms ◽  
Marine Organisms ◽  
Antimicrobial Compounds

Background: The treatment of infection caused by pathogenic bacteria becomes one of the serious concerns globally. The failure in the treatment was found due to the exhibition of multiple resistance mechanisms against the antimicrobial agents. Emergence of resistant bacterial species has also been observed due to prolong treatment using conventional antibiotics. To combat these problems, several alternative strategies have been employed using biological and chemically synthesized compounds as antibacterial agents. Marine organisms considered as one of the potential sources for the isolation of bioactive compounds due to the easily available, cost-effective, and eco-friendly. Methods: The online search methodology was adapted for the collection of information related to the antimicrobial properties of marine-derived compounds. These compound has been isolated and purified by different purification techniques, and their structure also characterized. Furthermore, the antibacterial activities have been reported by using broth microdilution as well as disc diffusion assays. Results: The present review paper describes the antimicrobial effect of diverse secondary metabolites which are isolated and purified from the different marine organisms. The structural elucidation of each secondary metabolite has also been done in the present paper, which will help for the in silico designing of the novel and potent antimicrobial compounds. Conclusion: A thorough literature search has been made and summarizes the list of antimicrobial compounds that are isolated from both prokaryotic and eukaryotic marine organisms. The information obtained from the present paper will be helpful for the application of marine compounds as antimicrobial agents against different antibiotic-resistant human pathogenic bacteria.

scholarly journals Comparative evaluation of the antioxidant, antimicrobial and nutritive properties of gluten-free flours

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joanna Miedzianka ◽  
Katarzyna Drzymała ◽  
Agnieszka Nemś ◽  
Agnieszka Kita
Keyword(s):  
Antimicrobial Properties ◽  
Corn Germ ◽  
Gluten Free ◽  
Evening Primrose ◽  
Oilseed Processing ◽  
Oilseed Cake ◽  
Microbial Growth Inhibition ◽  
Cold Pressed ◽  
Seed Flours ◽  
By Products

AbstractGluten-free flours are interesting alternative to wheat flours. They could be by-products of oilseed processing, characterized by high content of bioactive compounds. Therefore the aim of the study was to determine the antioxidant, antimicrobial properties, amino acid and fatty acid profile of flours obtained as by-products from the oil industry. The highest total polyphenol content and antioxidant activity was found to have evening primrose flour. The widest spectrum of microbial growth inhibition was indicated for corn germ extract which showed no antimicrobial activity only against Bacillus subtilis. The highest protein content was found in pumpkin, peanut and almond flours (more than 50 g/100 g). The major abundant amino acids in all the analysed oilseed cake flours were aspartic acid, glutamic acid and arginine. The analysed gluten-free flours were found to be a good source of polyunsaturated fatty acids (PUFAs), which comprised mainly linoleic acid and α-linolenic acid, whereas the best source of PUFAs was evening primrose flour. The results suggest that the cold-pressed seed flours possess valuable chemical composition and may be considered for improvement of the nutritional properties of food products.

scholarly journals Biofilm Inhibition and Antimicrobial Properties of Silver-Ion-Exchanged Zeolite A against Vibrio spp Marine Pathogens

2021 ◽  
Vol 11 (12) ◽  
pp. 5496
Author(s):  
Zarina Amin ◽  
Nur Ariffah Waly ◽  
Sazmal Effendi Arshad
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Antimicrobial Properties ◽  
Immune Defense ◽  
Silver Ion ◽  
Bacterial Disease ◽  
Zeolite A ◽  
Biofilm Inhibition ◽  
Volcanic Origin ◽  
Scanning Electron

A challenging problem in the aquaculture industry is bacterial disease outbreaks, which result in the global reduction in fish supply and foodborne outbreaks. Biofilms in marine pathogens protect against antimicrobial treatment and host immune defense. Zeolites are minerals of volcanic origin made from crystalline aluminosilicates, which are useful in agriculture and in environmental management. In this study, silver-ion-exchanged zeolite A of four concentrations; 0.25 M (AgZ1), 0.50 M (AgZ2), 1.00 M (AgZ3) and 1.50 M (AgZ4) were investigated for biofilm inhibition and antimicrobial properties against two predominant marine pathogens, V. campbelli and V. parahemolyticus, by employing the minimum inhibitory concentration (MIC) and crystal violet biofilm quantification assays as well as scanning electron microscopy. In the first instance, all zeolite samples AgZ1–AgZ4 showed antimicrobial activity for both pathogens. For V. campbellii, AgZ4 exhibited the highest MIC at 125.00 µg/mL, while for V. parahaemolyticus, the highest MIC was observed for AgZ3 at 62.50 µg/mL. At sublethal concentration, biofilm inhibition of V. campbelli and V. parahemolyticus by AgZ4 was observed at 60.2 and 77.3% inhibition, respectively. Scanning electron microscopy exhibited profound structural alteration of the biofilm matrix by AgZ4. This is the first known study that highlights the potential application of ion-exchanged zeolite A against marine pathogens and their biofilms.

scholarly journals Sputter-Deposited Ag Nanoparticles on Electrospun PCL Scaffolds: Morphology, Wettability and Antibacterial Activity

Coatings ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 345
Author(s):  
Daniele Valerini ◽  
Loredana Tammaro ◽  
Roberta Vitali ◽  
Gloria Guillot ◽  
Antonio Rinaldi
Keyword(s):  
Ag Nanoparticles ◽  
Food Packaging ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Deposition Process ◽  
Polymer Fibers ◽  
Antibacterial Action ◽  
Porous Scaffolds ◽  
Electrospinning Technique ◽  
Sputtering Deposition

Porous scaffolds made of biocompatible and environmental-friendly polymer fibers with diameters in the nano/micro range can find applications in a wide variety of sectors, spanning from the biomedical field to textiles and so on. Their development has received a boost in the last decades thanks to advances in the production methods, such as the electrospinning technique. Conferring antimicrobial properties to these fibrous structures is a primary requirement for many of their applications, but the addition of antimicrobial agents by wet methods can present a series of drawbacks. In this work, strong antibacterial action is successfully provided to electrospun polycaprolactone (PCL) scaffolds by silver (Ag) addition through a simple and flexible way, namely the sputtering deposition of silver onto the PCL fibers. SEM-EDS analyses demonstrate that the polymer fibers get coated by Ag nanoparticles without undergoing any alteration of their morphological integrity upon the deposition process. The influence on wettability is evaluated with polar (water) and non-polar (diiodomethane) liquids, evidencing that this coating method allows preserving the hydrophobic character of the PCL polymer. Excellent antibacterial action (reduction > 99.995% in 4 h) is demonstrated against Escherichia coli. The easy fabrication of these PCL-Ag mats can be applicable to the production of biomedical devices, bioremediation and antifouling systems in filtration, personal protective equipment (PPE), food packaging materials, etc.

scholarly journals Silver (I) N-Heterocyclic Carbene Complexes: A Winning and Broad Spectrum of Antimicrobial Properties

2021 ◽  
Vol 22 (5) ◽  
pp. 2497
Author(s):  
Filippo Prencipe ◽  
Anna Zanfardino ◽  
Michela Di Napoli ◽  
Filomena Rossi ◽  
Stefano D’Errico ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Resistance Mechanisms ◽  
Heterocyclic Carbenes ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Development Of Resistance ◽  
Starting Point ◽  
Catalytic Chemistry

The evolution of antibacterial resistance has arisen as the main downside in fighting bacterial infections pushing researchers to develop novel, more potent and multimodal alternative drugs.Silver and its complexes have long been used as antimicrobial agents in medicine due to the lack of silver resistance and the effectiveness at low concentration as well as to their low toxicities compared to the most commonly used antibiotics. N-Heterocyclic Carbenes (NHCs) have been extensively employed to coordinate transition metals mainly for catalytic chemistry. However, more recently, NHC ligands have been applied as carrier molecules for metals in anticancer applications. In the present study we selected from literature two NHC-carbene based on acridinescaffoldand detailed nonclassicalpyrazole derived mono NHC-Ag neutral and bis NHC-Ag cationic complexes. Their inhibitor effect on bacterial strains Gram-negative and positivewas evaluated. Imidazolium NHC silver complex containing the acridine chromophore showed effectiveness at extremely low MIC values. Although pyrazole NHC silver complexes are less active than the acridine NHC-silver, they represent the first example of this class of compounds with antimicrobial properties. Moreover all complexesare not toxic and they show not significant activity againstmammalian cells (Hek lines) after 4 and 24 h. Based on our experimental evidence, we are confident that this promising class of complexes could represent a valuable starting point for developing candidates for the treatment of bacterial infections, delivering great effectiveness and avoiding the development of resistance mechanisms.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

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