scholarly journals Effectiveness Test of Basil Leaf (Ocimum basilicum) Extract As Bioinsecticide In Mosquito Coil to Mosquito Aedes aegypti Death

2017 ◽  
Vol 3 (2) ◽  
pp. 6
Author(s):  
Indri Ramayanti ◽  
Kamalia Layal ◽  
Putri Utami Pratiwi
Keyword(s):  
Aedes Aegypti ◽  
Essential Oils ◽  
Vector Control ◽  
Dengue Fever ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ocimum Basilicum ◽  
Mosquito Coil ◽  
Active Compounds

Aedes aegypti is the vector of Dengue Haemorhagic fever. One of the method to prevent the spread of dengue fever is vector control. Principal activity to control this vector might decrease their population as transmitter of disease. One way of controlling mosquitoes vectors is using a bioinsecticide from basil leaf. Basil leaf extract has bioinsecticide effect to Aedes aegypti because basil leaf has some active compounds, such as Flavonoid, Saponin, Tannin and essential oils. These compound are toxic to mosquitoes. The purpose of this study was to determine the effectiveness of basil leaf (Ocimum basilicum L.) extract as mosquito coil against Aedes aegypti. The design of this study is xperimental study with 450 subjects of Aedes aegypti. Subjects were divided into six groups. They were 0% (negative control), 25%, 50%. 70%, 90% extract and Transfluthrin 0,03% (positive control). Each group contained 25 mosquitoes. Observation were carried out for 60 minutes with 10 minutes intervals and three times of repetition. The Data were analyzed by probit to calculate the value of Lethal consentration. The result of Probit Analyze showed that LC50 was at a concentration of 41,81% and LC90 was at concentration of 101.66%. The results showed that the extract of basil leaf (Ocimum basilicum L.) effective as Aedes aegypti bioinsecticide in mosquito coil.

scholarly journals AKTIVITAS EKSTRAK RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) SEBAGAI LARVASIDA TERHADAP Aedes aegypti DI KECAMATAN KELAPA LIMA KOTA KUPANG

2020 ◽  
Vol 8 (1) ◽  
pp. 54-68
Author(s):  
Deswandi W. S. Berri ◽  
Julianty Almet ◽  
Diana Agustiani Wuri
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Research Method ◽  
Hemorrhagic Fever ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Mosquito Vector ◽  
Curcuma Xanthorrhiza ◽  
Mosquito Vector Control

Dengue hemorrhagic fever (DHF) is a disease that is found in some tropical and subtropical regions. This disease is caused by dengue virus and is transmitted to humans through the bite of the Aedes aegypti mosquito. the solution taken in controlling DHF is to break the life cycle of the Aedes aegypti mosquito. Vector control is generally carried out using synthetic larvicides, namely abate / temefos, but the use of abate can cause residues, environmental pollution, poisoning and resistance of the eradicated vectors so that natural larvasides from plants are needed namely temulawak rhizome (Curcuma xanthorrhiza Roxb.) for vector control. The purpose of this study was to determine whether temulawak rhizome extract was effective or not in killing Aedes aegypti larvae. This research method includes larva collection, identification and maintenance of mosquitoes, determining sample size, making extracts and testing effectiveness. This study used a control and experiment group with 3 repetitions in the minutes to 15, 30, 45, 60 and 1440 (24 Hours). The control group was positive control using abate and negative control using aquades while the eksperiment group used extract of temulawak rhizome (Curcuma xanthorrhiza Roxb.) with concentrations of 0.6%, 0.8%, 1%, 1.2% and 1.5%. The results of this study indicate that the temulawak rhizome extract (Curcuma xanthorrhiza Roxb.) effective as larvicides because at the lowest concentration 0.6% can kill 100% Aedes aegypti larvae.

scholarly journals AKTIVITAS REPELLENT FORMULASI SEDIAAN SPRAY KOMBINASI MINYAK ATSIRI SERAI (Cymbopogon winterianus), DAUN KEMANGI (Ocimum basilicum) DAN NILAM (Pogostemon Cablin) BESERTA UJI PREFERENSINYA

2021 ◽  
Vol 6 (1) ◽  
pp. 87-97
Author(s):  
Faikah Dyah Utami ◽  
◽  
Arif Budi Setianto
Keyword(s):  
Essential Oils ◽  
Emulsion Stability ◽  
Positive Control ◽  
Negative Control ◽  
Ocimum Basilicum ◽  
Test Patch ◽  
Power Test ◽  
Pogostemon Cablin ◽  
Cymbopogon Winterianus ◽  
Mosquito Repellents

Plants that are played as mosquito repellents, one of which is plants that contain essential oils, such as lemongrass, basil and patchouli. As an effort to make it easier to use, this research will make a spray repellent preparation with a combination of active ingredients from essential oils of lemongrass, basil and patchouli. The research methods included making a spray repellent using essential oils of lemongrass, basil, patchouli every 0.1 ml (5 concentrations %), 0.2 ml (10% concentration), 0.3 ml (15% concentration) and the additives 1ml Propylene glycol and 5 ml 96% Ethanol which functions as a solvent. The experiment used five treatments, namely: Negative control (Kn), Formula A (0.1ml), Formula B (0.2ml), Formula C (0.3ml), and Positive Control (Kp). The parameters tested included pH test, patch test, organoleptic, specific gravity, emulsion stability, preference and percentage of protective power. The protective power test was carried out 10 times from each treatment for 6 hours of observation (0-1 hours, 1-2 hours, 2-3 hours, 3-4 hours, 4-5 hours and 5-6 hours). The results showed that the best spray expulsion treatment was formula B with a concentration of 10% (0.2 ml)

scholarly journals THE EFFECTIVENESS OF HERBAL MOSQUITO COILS “MORIZENA” AGAINST Aedes Aegypti DEATH

2018 ◽  
Vol 7 (2) ◽  
pp. 50 ◽  
Author(s):  
Rina Priastini Susilowati ◽  
Win Darmanto ◽  
Nanik Siti Aminah
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Acetylcholinesterase Activity ◽  
Positive Control ◽  
Negative Control ◽  
Seed Extract ◽  
Experimental Animals ◽  
Passiflora Foetida ◽  
One Way Anova ◽  
Lc50 And Lc90

It has been successfully found new herbal mosquito coils called “Morizena” made from Passiflora foetida leaf extract (40%), Chrysantemum cinerariaefolium flowers seed extract (40%), essential oils of  leaves-stems of Cymbopogon nardus (20%). The herbal mosquito coils are more effective to kill Aedes aegypti compared to commercial synthetic mosquito coils made from Transfluthrin. Treatment of exposure to herbal mosquito coils “Morizena” on Aedes aegypti for 8 hours/day with concentration 500 ppm (P1), 1000 ppm (P2), 2000 ppm (P3), 3000 ppm (P4), 4000 ppm (P5) and treatment of exposure to synthetic mosquito coils Transfluthrin 2500 ppm (K1) as a positive control, and without treatment of exposure to mosquito coils (K0) as a negative control. Experimental animals used were adult Ae. aegypti mosquito with 25 mosquitoes in each treatment. Experimental design used is one-way Anova test with linear regression to calculate its LC50 and LC90. The results of test to herbal mosquito coils “Morizena” given for 8 hours/day obtained mortality of Ae. aegypti by 92% to a concentration of 3000 ppm (P4) and 100% to a concentration of 4000 ppm (P5) and 100% for test to synthetic mosquito coils transfluthrin 2500 ppm (K1). Ae. aegypti LC50 and LC90 value for treatment of exposure to herbal mosquito coils “Morizena” are 999 ppm and 2977 ppm. Treatment of herbal mosquito coils “Morizena” with graded doses up to 4000 ppm and synthetic mosquito coils Transfluthrin 2500 ppm causing an increase in the enzyme acetylcholinesterase activity of Ae. aegypti. The conclusion is based on the Ae. aegypti  LC90 value is 2977 ppm, which means the effective dose of herbal mosquito coils “Morizena” to kill Ae. aegypti is 2977 ppm.

scholarly journals PENGARUH EKSTRAK DAUN PEPAYA (Carica papaya) TERHADAP KEMATIAN LARVA NYAMUK Aedes agypti INSTAR III

2019 ◽  
Vol 3 (1) ◽  
pp. 7-16
Author(s):  
Jonathan Payangka ◽  
Risma Risma ◽  
Prajogo Wibowo
Keyword(s):  
Aedes Aegypti ◽  
Dengue Fever ◽  
Carica Papaya ◽  
Mosquito Species ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Organophosphate Insecticide ◽  
Control Group Design

Background: Papaya leaves (Carica papaya) extract is a natural larvacide that contains papain and alkaloid karpain so its usage is safe for the environment. The larvacide properties can also be used to reduce the amount of Aedes aegypti mosquito larvae. This mosquito species is the main vector for the virus that causes dengue fever which incident number increases over the years. There have been a few methods used to control the mosquito’s amount, one of which is by decreasing the number of Aedes aegypti’s larvae using the organophosphate insecticide chemical known as temefos. Temefos is really effective in killing the Aedes aegypti larvae but it has a lot of side effects especially towards the environment. By controlling the number of the Aedes aegypti’s larvae, hopefully the number of the dengue fever case can also be reduced. Objective: To prove that the papaya leaves (Carica papaya) extract have effects on the death of Aedes aegypti’s instar III larvae. Method: This is an experimental research what uses a post test only control group design. The study consists of seven groups, which are positive control that is given abate powder, negative control that is given only aquadest, and five groups treated with concentrations of 0.5%, 1%, 1.5%, 2%, and 2.5%; repeated for four times. The calculation of the amount of dead larvae is done within the first 24 hours. Result: Based on the Kruskal-Wallis test, p’s value is <α with p value being 0.000 and α being 0.05. It proves the significance of the experiment. Conclusion: The papaya leaves (Carica papaya) extract have effects on the death of Aedes aegypti’s instar III larvae

scholarly journals EFIKASI EKSTRAK DAUN ZODIA (Evodia suaveolens) PELARUT HEKSANA DALAM MEMATIKAN LARVA INSTAR III Aedes aegypti

2020 ◽  
Vol 8 (2) ◽  
pp. 195
Author(s):  
Didi Setiyadi ◽  
Martini Martini ◽  
Mochamad Hadi
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Larval Mortality ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Environmental Damage ◽  
P Value ◽  
Control Group Design ◽  
Post Test

DHF is a disease caused by a virus which is transmitted through the bite of the Aedes aegypti mosquito and is one of the health problems in Indonesia. Efforts to decide the transmission of DHF by controlling the population of Aedes aegypti at the larval stage using larvaside temepos. Long-term use of temephos causes Aedes aegypti resistance and environmental damage. One of the plants that has the potential as botanical larvaside is the leaves of zodia (Evodia suaveolens). The purpose of this study was to prove the efficacy of zodia leaves of hexane solvent for killing A. aegypti larvae. This type of research is a pure experiment with the post test only control group design. The research sample is A. aegypti stage III susceptible strain. There are 7 groups consisting of 1 negative control (aquades), positive control (1 ppm temepos) and 5 treatment groups (zodia leaf extract). Data on larval mortality were counted 24 hours after treatment. The results of the one way ANOVA test showed that there were differences in mortality between test groups with a p value: 0.001 α (0.05). Zodia leaf extract probit test results showed LC50-24 hour and LC90-24 hour values of 0,443 ppm and 0,788 ppm. Zodia leaf extract with hexane solvent has efficacy as Aedes aegypti larvicide.

UJI IMUNOMODULATOR DARI EKSTRAK ETANOL DAUN WARU (HIBISCUS TILIACEUS) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT

2019 ◽  
Vol 1 (2) ◽  
pp. 11-16
Author(s):  
Yanna Rotua Sihombing ◽  
Debi Dinha Sitepu
Keyword(s):  
Treatment Group ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Delayed Type Hypersensitivity ◽  
Hypersensitivity Response ◽  
Delayed Type Hypersensitivity Response

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno®  32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno®  32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Uji daya hambat ekstrak daun awar-awar (ficus septica burm.f) terhadap pertumbuhan bakteri staphylococcus aureus dan eschericia coli

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Ismiranti D.A. Tuna ◽  
Pemsi M. Wowor ◽  
Henoch Awaloei
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infection ◽  
Natural Science ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
University Faculty ◽  
Microbiology Laboratory ◽  
Causal Organism ◽  
Empiric Data

Abstract: Awar-awar (Ficus septica Burm.F) is one of the plants that used by comunities. Based from the empiric data, this plant is believed have efficacy as a drug and could treat diseases. Staphylococcus aureus dan Eschericia coli are the most frequent causal organism in human bacterial infection. This study aimed to determine whether there is inhibition of leaf extract awar-awar against Staphylococcus aureus and Eschericia coli. This was a laboratory experimental with Kirby-Bauer pitting modification at Phytochemistry and Microbiology Laboratory of Sam Ratulangi University Faculty of Mathematics and natural Science. Awar-awar leaf extract was obtained from maceration process with 96% ethanol. Consentration of thick extract that was used were 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml. ciprofloxacin was used as positive control,and aquades was used as negative control. The result showed that the average of inhibition diemeters by awar-awar leaves were 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml respectively are 27,5 mm, 24,3 mm, 22,3 mm, 21,8 in Staphylococcus aureus, whereas in Eschericia coli were 24,8 mm, 21 mm, 18,3 mm, 17 mm. Conclusion: Awar-awar leaf extract has a potential inhibion effect on the growth of Staphylococcus aureus and Eschericia coli.Keywords: awar-awar leaves extract, staphylococcus aureus, eschericia coli, inhibition test. Abstrak: Tanaman awar-awar merupakan salah satu tanaman yang digunakan masyarakat untuk pengobatan. Berdasarkan data empiris, tanaman ini dipercaya memiliki khasiat sebagai obat dan bisa mengobati penyakit. Bakteri Staphylococcus aureus dan Eschericia coli merupakan penyebab infeksi utama pada manusia. Penelitian ini bertujuan untuk mengetahui ada tidaknya daya hambat ekstrak daun awar-awar terhadap bakteri Staphylococcus aureus dan Eschericia coli. Jenis penelitian ini ialah eksperimental laboratorium dengan modifikasi Kirby-Bauer sumuran di Laboratorium Fitokimia dan Mikrobiologi Fakultas MIPA Universitas sam Ratulangi. Ekstrak daun awar-awar diperoleh dari proses maserasi dengan etanol 96%. Konsentrasi ekstrak kental yang digunakan dalam penelitian ini ialah 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml. Siprofloksasin sebagai kontrol positif, dan akuades sebagai kontrol negatif. Hasil penelitian mendapatkan rata-rata diameter zona hambat ekstrak daun awar-awar konsentrasi 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml berturut-turut ialah 27,5 mm, 24,3 mm, 22,3 mm, 21,8 mm pada bakteri Staphylococcus aureus, sedangkan pada bakteri Eschericia coli ialah 24,8 mm, 21 mm, 18,3 mm, 17 mm. Simpulan: Ekstrak daun awar-awar berpotensi memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dan Eschericia coli. Kata kunci: ekstrak daun awar-awar, staphylococcus aureus, eschericia coli, uji daya hambat.

scholarly journals UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 44-53
Author(s):  
Andri Priyoherianto ◽  
◽  
Panji Ratih Suci ◽  
Putri Rizki Cahya Fatimah ◽  
Atik Nur Wijayanti
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Water Hyacinth ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Normal Limits ◽  
Blood Glucose Levels ◽  
Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

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