Pengaruh Kejutan Suhu Panas Dan Lama Waktu Setelah Pembuahan Terhadap Daya Tetas Dan Abnormalitas Larva Ikan Nila (Oreochromis niloticus) [The Thermal Temperature Shock And Time After Fertilization In Hatching Rate And Abnormality Of Nile Fish (Oreochromis niloticus) ]

AbstractThe chromosome manipulation in fish is one of the strategy that will be used to produce of the high generation and good quality in its genetics, as follow fast to grow, disease resistant, high survival, tolerate with environment exchange and easy to be cultured. One of manipulation chromosome the method in fish used thermal temperature shock. The aim of the study was to know the thermal temperature shock and the time after fertilization forward hatching rate and abnormality of nile fish larvae and also to know the thermal temperature shock and the time after fertilization that resulted the best hatching rate and abnormality in nile fish larvae. The method was Factorial Completed Randomized Design as experimental design. The treatment used temperatures shock were 40ºC, 41ºC and 42ºC as long as 2,5 minutes, with the time of 80, 85 and 90 minutes after fertilization, each treatment was four replications. The primary parameter was hatching rate and abnormality of nile fish larvae and the secondary parameter were fertilization and water quality. The result showed that the thermal temperature shock was significantly influenced to hatch rate and abnormality, but the time of shocking after fertilization and the interaction between them showed were not significantly influenced to hatch rate and abnormality. The highest of hatching rate and abnormality percentage was showed by the treatment with shocking 40°C was 90,77 percent and 2,60 percent.

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Daya Tetas Telur dan Sintasan Larva Dari Hasil Penambahan Madu pada Bahan Pengencer Sperma Ikan Nila (Oreochromis niloticus)

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.3.1.2015.6950 ◽

2019 ◽

Vol 3 (1) ◽

Author(s):

Yunus Ayer ◽

Joppy Mudeng ◽

Hengky Sinjal

Keyword(s):

Experimental Design ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Larval Survival ◽

Hatching Rate ◽

Dilution Ratio ◽

Egg Hatching ◽

Research Results ◽

Spermatozoa Motility ◽

Randomized Design

The objective of research was to determine the concentration of honey in sperm dilution for improving hatching rate of egg and survival of nile tilapia larvae (Oreochromis niloticus).The number of fish used was five pairs broodstock. Dilution substance was NaCl and honey. Observations were conducted on spermatozoa motility, fertility and egg hatching rate. Experimental design used was complete randomized design. Sperm dilution substance was prepare by disolving honey (0 mL; 0,60 mL; 0,65 mL dan 0,70 mL) in 100 mL; 99,40 mL; 99,35 mL and 99,30 mL NaCl respectively. Each dilution was homogenized using aerator for 15 minutes. Fertilization was done 12 hours after ovulation. Egg hatching rate was observed after ovulation. Research results showed the use of honey had significant effet on egg hatching rate, but not on larval survival. Treatment D (0,70 mL honey in 99,30 mL NaCl) had the highest hatching rate (77.33%). Dilution ratio 1:60 was the best indicated by spermatozoa motility 96.66%, fertility 71.65, hatching rate 70% and larval survival 81.67% Keywords : honey, egg hatching rate, larval survival, Oroechromnis niloticus

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Pengaruh suhu terhadap perkembangan embrio ikan Cupang Betta splendens

DEPIK ◽

10.13170/depik.8.3.13916 ◽

2019 ◽

Vol 8 (3) ◽

pp. 235-242

Author(s):

Mustaqim Mustaqim ◽

Kartini Eriani ◽

Erlangga Erlangga ◽

Rachmawati Rusyidi

Keyword(s):

Water Quality ◽

Fish Larvae ◽

Fertilization Rate ◽

Quality Parameters ◽

Betta Splendens ◽

Effect Of Temperature ◽

Hatching Rate ◽

Egg Hatchability ◽

Hatching Time ◽

Randomized Design

Abstract. Temperurate is one of the important parameter in the embryo development of fish; However, to date no information this effect on the Betta splendens. Hence, this study aims to determine the effect of temperature on egg hatchability and survival of betta fish larvae. Samples of betta fish used in this study are similar in size and from the same type of broodstock. A completely randomized design used with four treatments and three replications. The parameters observed were the process of egg development, fertilization rate, hatching rate, hatching duration, survival rate, and water quality (pH, DO, temperature). The results showed that the relation between temperature and fertilization rate was not significantly different, whereas the calculated F value (0.23) ˂ F table 0.05 (4.07). The relation between temperature and hatching rate has a very significant effect, F value (18.91) ˃ F table 0.05 (4.07). The relation of temperature to hatching time showed the same difference, and the relation of temperature to survival of betta fish (Betta spelendes) larvae is significant with F ¬ values (39.98) F table 0.05 (4.07). Water quality parameters during the study were pH 7 - 7.5, DO 5.0 - 7.7 ppm and temperature at 27 - 29 oC.Keywords: Betta fish eggs, temperature, embryogenesis Abstrak. Suhu adalah salah satu faktor menentukak proses perkembangan embrio pada ikan, namun pengaruh ini belum pernah diamati pada ikan cupang Betta splendens. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap perkembangan embrio, daya tetas telur dan kelangsungan hidup larva ikan cupang. Induk ikan cupang yang digunakan memiliki ukuran yang sama dan dari induk jenis yang sama. Adapun rancangan yang digunakan dalam penelitian adalah rancangan acak non factorialdengan 4 perlakuan 3 kali ulangan. Parameter yang diamati adalah proses perkembangan telur, tingkat pembuahan telur, tingkat penetasan telur, lama penetasan telur, tingkat kelangsungan hidup dan kualitas air pH, DO, suhu ruangan. Hasil penelitian menunjukkan bahwa hubungan suhu terhadap tingkat pembuahan telur tidak berbedanyata, dimana nilai F hitung (0,23) ˂ F tabel 0,05 (4,07). Hubungan suhu terhadap tingkat penetasan telur berpengaruh sangat berbedanyata, dimana nilai F hitung (18,91) ˃ F tabel 0,05 (4,07). Hubungan suhu terhadap lama penetasan menunjukkan berbeda sama, dan hubungan suhu terhadap kelangsungan hidup berpengaruh nyata terhadap kelangsungan hidup larva ikan cupang (Betta spelendes) dengan nilai F hitung (39,98) F tabel 0,05 (4,07). Parameter kualitas air selama penelitian rata-rata pH 7 – 7,5, DO 5,0 – 7,7 ppm dan suhu ruangan 27 – 29 oC.Kata kunci: Telur ikan cupang, suhu, embriogenesis

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Variasi Lama Kejutan Panas pada Suhu yang Sama terhadap Tingkat Penetasan Telur (HR) Ikan Lele Dumbo (Clarias gariepinus)

Al-Hayat: Journal of Biology and Applied Biology ◽

10.21580/ah.v3i1.6068 ◽

2020 ◽

Vol 3 (1) ◽

pp. 37

Author(s):

Anny Rimalia

Keyword(s):

Heat Shock ◽

Clarias Gariepinus ◽

Shock Duration ◽

African Catfish ◽

Hatching Rate ◽

Egg Hatching ◽

Temperature Shock ◽

Randomized Design ◽

Completely Randomized Design ◽

Different Climates

Species, temperature shock duration, fertilization temperature from different climates can cause different hatching results, so it is necessary to do research on the treatment of variations in heat shock at the same temperature, to obtain information about the hatching rate (HR) of African catfish (Clarias gariepinus). The method used is completely randomized design (3x3). The results of the egg hatching rate (HR), with variations in the heat shock at the same temperature (35⁰C), showed the highest rate of hatching dumbo catfish eggs at heat shock one minute 233.00%, then heat shock two minutes 212.00%, and heat shock three minutes 200.00% respectively.

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Masculinization of Tilapia (Oreochromis niloticus) Using Methyl Testosterone Natural Hormone

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v8i2.3347 ◽

2014 ◽

Vol 10 (2) ◽

Author(s):

Rosmaidar R ◽

Winaruddin W ◽

Mimi Herlina

Keyword(s):

Oreochromis Niloticus ◽

Fish Larvae ◽

Hormone Treatment ◽

Control Fish ◽

Control Group ◽

Group Iii ◽

Randomized Design ◽

Natural Hormone ◽

Male Sex ◽

Completely Randomized Design

This study aimed to determine the effect of methyl testosterone natural hormoneon the rate of masculinization of tilapia (Oreochromis niloticus). The study used 270 tilapia larvae with the age of 7 days as experimental animals. Tilapia larvae were divided into 3 treatments; eachtreatment consisted of 30 larvae fish. This study was an experimental study using completely randomized design with 3 treatments and 3replications. Treatment I as control group was fed with pellets ad libitum, treatment II was given feed containing methyl testosterone naturalhormone, and treatment group III was given hormone treatment by soaking the fish larvae with methyl testosterone natural hormone for 20hours. Larvae were raised in accordance with applicable procedures. Observation was conducted when the larvae fish were at 60 days old andthe sex of fish can be observed. Parameter examined was the percentage of the male sex. The average (± SD) percentage of male tilapia in groupPI, PII, and PIII were 36.39±3.97, 66.72±6.13, and 82.37±6.87%, respectively. The results showed that the percentage of male sex of tilapia fedwith natural hormone of methyl testosterone through the diet and dipping was significantly different (P0.05) from control fish. In conclusion,the administration of natural hormone of methyl testosterone increased the percentage of the male sex of tilapia).____________________________________________________________________________________________________________________Key words: tilapia larvae, natural hormone methyl testosterone, the male sex percentage

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Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.5.1.2017.14836 ◽

2017 ◽

Vol 5 (1) ◽

Author(s):

Erwin A. Aziz ◽

Ockstan Kalesaran

Keyword(s):

Survival Rate ◽

Experimental Design ◽

Aromatase Inhibitor ◽

Fish Larvae ◽

Clarias Gariepinus ◽

Randomized Design ◽

Completely Randomized Design ◽

The Difference ◽

Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae. Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

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EFFECTIVENESS Daphnia sp. WHICH IS PELET FEEDING TO THE GROWTH OF SEMAH’S LARVAE Tor douronensis (Valenciennes, 1842)

Journal of Aquatropica Asia ◽

10.33019/aquatropica.v5i2.2069 ◽

2020 ◽

Vol 5 (2) ◽

pp. 23-27

Author(s):

Dina Tri Madya Ningsih ◽

Siti Hudaidah ◽

Mas Tri Djoko Sunarno

Keyword(s):

Survival Rate ◽

Fish Larvae ◽

Weight Growth ◽

Randomized Design ◽

Test Parameters ◽

Absolute Growth ◽

Absolute Length ◽

Effective Doses ◽

Endemic Fish ◽

Completely Randomized Design

The semah fish (Tor douronensis) is an Indonesian endemic fish that has a long growth, which takes 4 years to grow from larvae to broodstock. This study aims know of Daphnia sp. pelet fed with effective doses to increase the growth of semah’s fish larvae. The study was conducted from March 25 to April 13, 2020 at the Instalasi Riset Plasma Nutfah, Cijeruk, Bogor, West Java. The experimental design used completely randomized design (CRD) 4 treatments and 3 replications, namely treatment A (Daphnia sp.+pelet feed 0 gr/ L), treatment B (Daphnia sp.+ pelet feed 1 gr / L), treatment C (Daphnia sp. + pelet feed 2 gr / L), treatment D (Daphnia sp. + pelet feed 3 gr / L). The larvae of 9 days in age were randomly stocked into 12 aquariums measuring 60x30x35 cm with a density of 50 fish/aquarium and fed in the form of Daphnia sp. enriched pelet feed according to the treatment of 50 ind/larvae by giving 3 times a day which is maintained for 20 days. The test parameters measured are absolute growth in length and weight, survival rate, and water quality. Based on the results of statistical analysis (Anova), the results showed that the treatment D gave the highest influence on the growth of semah’s fish larvae (P <0.05) with an absolute weight growth value of 85,3 mg, absolute length growth of 10.6 mm, and survival rate 99.33%.

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ANALISIS KARAKTER REPRODUKSI DAN PERFORMA BENIH HIBRID IKAN NILA PANDU F6 DENGAN IKAN NILA NILASA (Oreochromis niloticus) Analysis of the Character Reproduction and Seed Hybrid Performance 0f Tilapia Fish F6 Pandu with Nilasa Tilapia (Oreochromis niloticus)

SAINTEK PERIKANAN Indonesian Journal of Fisheries Science and Technology ◽

10.14710/ijfst.12.1.19-23 ◽

2017 ◽

Vol 12 (1) ◽

pp. 19

Author(s):

Dio Patria Yustysi ◽

Fajar Basuki ◽

Titik Susilowati ◽

Tristiana Yuniarti

Keyword(s):

Water Quality ◽

Growth Rate ◽

Survival Rate ◽

Specific Growth Rate ◽

Oreochromis Niloticus ◽

Egg Size ◽

Specific Growth ◽

Fish Farming ◽

Hatching Rate ◽

Feed Conversion

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh dari hibridisasi ikan nila Pandu F6 dengan nila Nilasa terhadap karakter reproduksinya dan performa benih yang dihasilkan. Penelitian ini dilakukan dengan metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan. Perlakuan dalam penelitian ini: perlakuan A (Pandu F6 ♀><Pandu F6 ♂), B (Nilasa ♀><Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), dan D (Nilasa ♀><Pandu F6 ♂). Data yang diamati meliputi fekunditas, daya tetas telur, diameter dan bobot telur, panjang dan bobot larva TL, panjang dan bobot larva lepas kuning telur, kelulushidupan, laju pertumbuhan spesifik, konversi pakan dan kualitas air. Hasil penelitian menunjukan nilai terbaik pada fekunditas sebesar 1191,67 ± 239,45 butir/200 g, daya tetas telur sebesar 80,93 ± 3,90%, kelulushidupan sebesar 81,55 ± 7,54%, laju pertumbuhan spesifik sebesar 7,26 ± 0,25, konversi pakan 0,54 ± 0,02 didapatkan pada perlakuan C, akan tetapi untuk diameter dan bobot telur, panjang dan bobot larva Kuning Telur, dan panjang dan bobot larva lepas kuning telur tidak berbeda nyata. Hasil tersebut menunjukan bahwa hibridisasi (perlakuan C) memberikan pengaruh yang nyata (P < 0,05) terhadap fekunditas, daya tetas telur, kelulushidupan, laju pertumbuhan spesifik, dan konversi pakan tetapi tidak berpengaruh terhadap diameter dan bobot telur, panjang dan bobot larva, dan panjang dan bobot larva lepas kuning telur. Kata kunci: Hibridisasi, Ikan Nila, Karakter Reproduksi, Performa Benih ABSTRACT This research aims to know the influence of hybridization tilapia fish Pandu F6 with nila Nilasa against the character of the reproduction and seed performance. This research was conducted with the experimental method using Random Design complete (RAL) with 4 treatments and three replicates. The treatments in this study: the treatment A (Pandu F6 F6 ♀ >< Pandu ♂), B (Nilasa ♀>< Nilasa ♂), C (Pandu F6 ♀>< Nilasa ♂), and D (Nilasa ♀ >< Pandu F6 ♂). The observed data covering fecundity, hatching rate, egg size, yolk sack larva length and weight, length and weight of the egg yolk off larvae, survival rate, specific growth rate, feed conversion rate, and water quality. The results showed the best value on the fecundity of 1191.67±239.45 eggs/200 g, Hatching rate 80.93 ± 3.90%, Survival rate of 81.55 ± 7.54%, specific growth rate of 7.26 ± 0.25, conversion fodder 0.54 ± 0.02 obtained at the treatment C, but for the diameter and weight of egg, larval length and weight TL, and the length and weight of the yolk egg off larvae for each treatment do not differ markedly. The results showed that hybridization (treatment C) gives a real influence (P < 0.05) of fecundity,hatching rate, survival rate but not with the egg size, larva weight and length, and the length and weight of larvae off yolk. Water quality on the spawning, hatching eggs and larvae found on the maintenance of a decent range for tilapia fish farming Keywords: hybridization, tilapia fish, character of the reproduction, seed performance

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The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS ◽

10.36987/jpbn.v6i1.1597 ◽

2020 ◽

Vol 6 (1) ◽

pp. 88-95

Author(s):

Marlina Doloksaribu ◽

Robert Martua Simanjuntak ◽

Ied Hidayani Parinduri

Keyword(s):

Social Sciences ◽

Experimental Study ◽

Data Analysis ◽

Survival Rate ◽

Cyprinus Carpio ◽

Oreochromis Niloticus ◽

Fish Farming ◽

Randomized Design ◽

The Social ◽

Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

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PENGARUH PEMBERIAN PROBIOTIK Bacillus spp. MELALUI PAKAN TERHADAP KELANGSUNGAN HIDUP DAN LAJU PERTUMBUHAN IKAN NILA (Oreochromis niloticus)

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v16i1.212 ◽

2016 ◽

Vol 16 (1) ◽

Author(s):

Muhammad Masyarul Rusdani, Sadikin Amir Saptono Waspodo dan Zaenal Abidin

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Oreochromis Niloticus ◽

Specific Growth ◽

Good Combination ◽

Randomized Design ◽

Bacillus Spp ◽

Completely Randomized Design ◽

Total Bacteria ◽

Different Doses

ABSTRAKPenelitian ini bertujuan untuk menguji kinerja bakteri probiotik Bacillus spp. dan prebiotik molase dengan dosis berbeda terhadap tingkat kelangsungan hidup dan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Penelitian ini menggunakan motede eksperimen dengan rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan, yaitu kontrol (K), Bacillus spp. 10 ml/kg pakan (A); Bacillus spp. 10 ml/Kg pakan + molase 10 v/v (B); Bacillus spp. 20 ml/Kg pakan (C); dan Bacillus spp. 20 ml/Kg pakan + molase 10 v/v(D). Hasil yang diperoleh menunjukkan variasi untuk total bakteri, yaitu dari 2,24x108 hingga 3,78x108 CFU/ml. Demikian juga dengan total Bacillus spp. dari 1,07x106 hingga 2,02x107 CFU/ml. Adapun untuk pengamatan tingkat kelangsungan hidup dan laju pertumbuhan tidak menunjukkan hasil yang signifikan. Meski demikian, perlakuan D memiliki hasil yang relatif lebih baik dengan nilai SR dan LPH berturut-turut 96,67% dan 1,01 %/hari. Kata kunci : probiotik Bacillus spp., prebiotik molase, total bakteri dan Bacillus spp., tingkat kelangsungan hidup, laju pertumbuhan harian ABSTRACTThis study was aimed to determine the good combination of probiotic bacillus spp. and prebiotic molasses with different doses to survival rate and growth performance of tilapia (Oreochromis niloticus). this study has used experimental design with completely randomized design (CRD) and five treatment, i.e. Control (K), without probiotic and prebiotic; Bacillus spp. 10 ml/kg feed (A); Bacillus spp. 10 ml/Kg feed + molasses 10 v/v (B); Bacillus spp. 20 ml/Kg feed (C); dan Bacillus spp. 20 ml/Kg feed + molasses 10 v/v (D). The results showed variation for total bacteria and total Bacillus spp. The average of total bacteria obtained in this study ranged from 2,24x108 to 3.78x108 CFU/ml. Likewise, the total Bacillus spp. were obtained from 1.07x106 to 2.02x107 CFU/ml. While for survival rate (SR) and the growth rate (DGR) did not showed significant results. However, treatment D has a relatively better then treatment else with SR and DGR respectively 96.67% and 1.01%.day-1. Keywords : probiotic Bacillus spp., prebiotic molasses, total bacteria and Bacillus spp., survival rate, specific growth rate.

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PENGARUH PEMBERIAN ENZIM DENGAN KONSENTRASI BERBEDA PADA PAKAN IKAN NILA (Oreochromis niloticus) TERHADAP KONSENTRASI AMONIAK, NITRIT, DAN SULFIDA DALAM MEDIA PEMELIHARAAN (The Influence of Enzime Provision with Different Concentration of Enzimes on Tilapia Fish Feed (Oreochromis niloticus) Towards The Concentration of Ammonia, Nitrite, and Sulfide in Media Maintenance)

Management of Aquatic Resources Journal (MAQUARES) ◽

10.14710/marj.v6i4.21345 ◽

2018 ◽

Vol 6 (4) ◽

pp. 530-535

Author(s):

Uswah Hasanah ◽

Haeruddin Haeruddin ◽

Niniek Widyorini

Keyword(s):

Oreochromis Niloticus ◽

Ammonia Concentration ◽

Fish Feed ◽

Aquatic Resources ◽

Digestion Process ◽

The People ◽

Sulphide Concentration ◽

Randomized Design ◽

Maintenance Time ◽

Water Volumes

Kegiatan budidaya pada setiap prosesnya menghasilkan limbah yang dihasilkan dari sisa-sisa pakan dan kotoran yang berasal dari ikan budidaya, terutama budidaya Ikan Nila yang merupakan salah satu jenis ikan tawar yang sudah di budidaya secara komersial oleh masyarakat indonesia. Penelitian dilaksanakan pada Mei-Juni 2017 di Laboratorium Pengelolaan Sumberdaya Ikan dan Lingkungan, Departemen Sumberdaya Akuatik, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro. Pada penelitian ini ditambahkan enzim pada pakan ikan, suatu enzim yang mengandung protease, lipase, amilase, pepsin, tripsin, dan kemotripsin dalam dosis yang sudah ditentukan untuk memaksimalkan proses pencernaan. Ikan Nila yang digunakan berukuran 7-9 cm dipelihara di dalam akuarium dengan kapasitas 2 ekor Ikan Nila dalam 1 akuarium dengan volume air 9 l. Tujuan penelitian untuk mengetahui konsentrasi amoniak, nitrit, dan sulfida (H2S) dan membandingkan pengaruh pemberian enzim dengan konsentrasi yang berbeda didalam pakan terhadap konsentrasi amoniak, nitrit, dan sulfida (H2S). Metode yang digunakan adalah metode eksperimental skala laboratorium. Desain penelitian berdasarkan rancangan acak lengkap (RAL), dengan menggunakan lima perlakuan yaitu dengan perbedaan konsentrasi enzim. Setiap perlakuan dilakukan 3x pengulangan. Analisis data menggunakan uji two way anova. Hasil penelitian menunjukkan bahwa pemberian enzim dengan konsentrasi berbeda tidak berpengaruh nyata, lama waktu pemeliharaan berpengaruh nyata terhadap konsentrasi amoniak, dan sulfida, kombinasi dari keduanya tidak berpengaruh nyata terhadap konsentrasi amoniak, nitrit, dan sulfida. Cultivation activities in each process produce waste generated from the remnants of feed and feces from the fish cultivated, especially the cultivation of tilapia which is one type of fresh bonds that have been cultivated commercially by the people of Indonesian. The research was conducted in May-June 2017 at the Fish and Environmental Resource Management Laboratory, Aquatic Resources Department, Fisheries and Marine Science Faculty, Diponegoro University. The materials on this research are combining the fish feed with an enzime which contains protease, lipase, amylase, pepsin, trypsin, and chemotrypsin in prescribed doses to maximised the digestion process. Tilapia as research object has length of 7 to 9 cm and its kept in an aquarium with capacity (water volumes) of 9 ls which contains of 2 tilapias. The purposes of this research are to know the concentration of ammonia, nitrite and sulfide (H 2 S) in and to compare the effect of the enzyme provision with different concentration in fish feed with the concentration of ammonia, nitrite and sulfide (H 2 S). This research used laboratory-scale experimental method. The study design was based on Complete Randomized Design (RAL), using five treatments with different enzyme concentrations. Each treatments was done by three repetitions. The data was analysed using two-way ANOVA test. The results showed that enzyme with different concentration had no significant effect, maintenance time had significant effect on ammonia concentration, and sulfide, the combination of both did not significantly affect the ammonia, nitrite and sulphide concentration.

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