Hepatoprotective and Curative Activities of Daphne gnidium L. Aqueous Extract Against CCl4-Induced Liver Injury in Rats

2020 ◽  
Vol 16 ◽  
Author(s):  
Ifriqya Medila ◽  
Ikram Toumi ◽  
Aicha Adaika
Keyword(s):  
Liver Injury ◽  
Dose Level ◽  
Aqueous Extract ◽  
Total Bilirubin ◽  
Relative Weight ◽  
Control Group ◽  
Albino Rats ◽  
Daphne Gnidium ◽  
Activity Test ◽  
Ccl4 Solution

Background:: Liver is one of the largest organs of the human body and the main site for intense metabolism and excretion. Liver injury or dysfunction is recognized as a serious worldwide health problem. Daphne gnidium L., medicinal plant widely distributed in Algeria and used by populations in traditional medicine in the treatment of hepatitis and other diseases. Objective:: Protective and curative effects of aqueous extract of Daphne gnidium L. leaves were investigated against carbon tetrachloride (CCl4) induced hepatic damage in rats (Wistar Albino). Method:: This study was conducted on 32 Albino rats, which were divided into four groups; the first served as control. Group II animals were treated intraperitoneally with CCl4 solution (3ml/kg). Group III animals were treated intraperitoneally with CCl4 solutions (3ml/kg) in the first day, and given aqueous Daphne extract orally at a dose level of (300 mg/kg) daily for 7 days (curative activity test). Animals of group IV were orally administered aqueous Daphne extract at a dose level of (300 mg/kg) daily, and on the seventh day treated intraperitoneally with CCl4 solution (3ml/kg) 3 hours after the latest extract administration (preventive activity test). Results:: Our results show that CCl4 significantly increases the relative liver weight, serum values of ALT, AST, ALP, GGT, and Total bilirubin, as well as the hepatic rate of oxidative stress parameters; MDA, CAT activities, and significantly decreases hepatic GSH levels compared to the control group. These results were confirmed by liver lesions observed in histological analyses. Pre-treatment and curative treatment of dose 300 mg / kg of aqueous Daphne gnidium L. extract significantly reduced relative weight, serum levels of ALT, AST, ALP, GGT and Total bilirubin liver rate, CAT activity and increases GSH level. In addition, normal liver architecture has been restored. Conclusion:: The present study demonstrates the hepatoprotective and curative effects of aqueous extract Daphne gnidium leaves against CCl4-induced liver injury in rats by improving the antioxidant status in tissues (liver) and ameliorating the harmful effects induced by CCl4.

scholarly journals A STUDY OF THE EFFECT OF THE PHYTOCHEMICAL CONTENT OF P. AMERICANALEAF ON HEMATOLOGICAL SYSTEM OF ALBINO RATS

2021 ◽  
Vol 9 (04) ◽  
pp. 464-467
Author(s):  
Ngozi a ◽  
◽  
N. Omeke ◽  
Haruna M. Ndahi ◽  
◽  
...  
Keyword(s):  
Adverse Effect ◽  
Experimental Study ◽  
Aqueous Extract ◽  
Leaf Extract ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Content ◽  
Other Substances ◽  
And Control ◽  
Experimental Group

Given the medicinal importance of the avocado plant in alternative medicine,the present study aimed to study the effect of the phytochemical content of P. americana leaf extract on the hematological system of albino rats. Albino rats were obtained andclustered into sixgroups,with five rats per groupsimultaneously were assigned experimental and control. The rats in the experimental group were administered with varying doses of the prepared aqueous extract of P. americanawithin the period of the study. The rats in the control group were administered with other substances such as water and feed for the study period.The hematological system of the experimental animal was assessed after the administration of the extract, and it was observed that the aqueous extract of P. americana leaf recorded no significant adverse effect on the hematology system of the experimental study animal.

scholarly journals Hepatoprotective effects of hydroethanolic extracts of Crocus sativus tepals, stigmas and leaves on carbon tetrachloride induced acute liver injury in rats

2021 ◽  
Vol 25 (2) ◽  
pp. 178-188
Author(s):  
Sabir Ouahhoud ◽  
◽  
Ilham Touiss ◽  
Amine Khoulati ◽  
Iliass Lahmass ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Total Bilirubin ◽  
Liver Weight ◽  
Crocus Sativus ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Relative Liver Weight ◽  
Hepatoprotective Effects

Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Malondialdehyde (MDA) was quantified in liver homogenate. Results: The experimental data showed that the stigmas and tepals extracts significantly prevented weight body loss and improved the relative liver weight. They significantly protected against elevation of ALT, AST, direct bilirubin, total bilirubin, LDH, ALP, creatinine and MDA. Also, they enhanced significantly total proteins and albumin compared to the CCL4 control group. Moreover, leaves reduced ALT, AST, total bilirubin, LDH and MDA significantly. Conclusion: In conclusion, these results suggest that tepals, stigmas, and leaves extracts of Crocus sativus have hepatoprotective effects on CCL4 induced liver injury in rats.

scholarly journals PROTECTIVE ROLE AND ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF ROSMARINUS OFFICINALIS AGAINST TRICHLOROACETATE-INDUCED TOXICITY IN LIVER OF MALE RATS

2018 ◽  
Vol 11 (6) ◽  
pp. 420
Author(s):  
Medhat Mostafa Abozid ◽  
Hoda Ea Farid
Keyword(s):  
Aqueous Extract ◽  
Liver Damage ◽  
Protective Role ◽  
Rosmarinus Officinalis ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Gamma Glutamyl Transferase ◽  
Group I ◽  
Glutamyl Transferase

 Objective: The current study was designed to estimate the potential protective role of the aqueous extract of rosemary (AER) (Rosmarinus officinalis) against trichloroacetic acid (TCA)-created hepatotoxicity in male albino rats.Methods: Forty male albino rats were separated into four groups of ten: Group I served as control; Group II was given AER (200 mg/kg/day) by gavage; Group III received TCA at the dose 50 mg/kg/day, and Group V was treated with AER (200 mg/kg/day) and received TCA (50 mg/kg/day). The experiment was carried out for 2 months.Results: The toxicity of TCA for rats was revealed by an elevation in liver marker enzymes activities (gamma-glutamyl transferase [GGT], alkaline phosphatase [ALP], aspartate transaminase [AST], alanine aminotransferase [ALT]) and conjugated bilirubin (CB) level, and a decrease in albumin and total protein (TP) levels. The TCA administration also caused a significant increase in the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), and also malondialdehyde (MDA) level in liver tissues. These biochemical effects were accompanied by histological indicators of liver damage. Treatment with ARE recovered the liver damage instigated by TCA, as showed by perfection of liver enzyme markers (GGT, ALT, AST, ALP), CB, TP and albumin; as well as antioxidant parameters (CAT, SOD, GPx) and lipid peroxidation (MDA) and amelioration of histopathology changes in the liver tissues.Conclusion: It could be concluded that AER supplementation for 2 months in TCA-induced toxicity in rats benefited hepatic antioxidant status and improved liver injury and damage in male albino rats exposed to TCA.

scholarly journals Antihyperglycemic and Antihyperlipidemic of Karala (Momordica charantia) Fruits in Streptozotocin Induced Diabetic Rats

2012 ◽  
Vol 5 (1) ◽  
pp. 29-37 ◽  
Author(s):  
MA Hossain ◽  
M Mostofa ◽  
D Debnath ◽  
AKMR Alam ◽  
Z Yasmin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Body Weight ◽  
Total Cholesterol ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Momordica Charantia ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Higher Doses

To investigate the antihyperglycemic and antihyperlipidemic effect of Momordica charantia (Karala), the aqueous extract of the Karala fruit was tested on streptozotocin (STZ)-induced diabetic rats. Thirty six albino rats were used in the experiment, 30 diabetic and the remaining six as negative control (T1). Diabetes was induced by administering (injecting) STZ at dose of 55mg/kg body weight. Thirty diabetic animals were randomly divided into five groups such as diabetic control group (T2) without any application of treatment, and groups T3,T4,T5 and T6 were treated with aqueous extract of Karala fruits daily at the doses of 250,    500 and 750mg/kg and glibenclamide (at a dose of 5mg/kg body weight) respectively. The body weight was taken and blood samples were collected from individual animal to determine glucose levels at 15 day interval up to 90 days. In addition, Asparate  Transaminenase(AST), Alanine Transaminenase(ALT), Alkaline Phosphatase(ALP), Total cholesterol (TCh) and Triglyceride (TGA) were determined at day 15 and at the end of the experiment. All three doses of Karala extracts reduced diabetic induced blood sugar and the reduction is comparable with standard glibenclamide (GLM) dose particularly with higher doses Karala extracts (500 and 750mg). Karala also prevented body weight loss due to induced diabetes as did by GLM treatment.. The treatment also resulted in a significant reduction of Asparate Transaminenase(AST), Alanine Transaminenase(ALT), Alkaline Phosphatase(ALP), Total cholesterol (TCh) and Triglyceride (TGA) activities of treated rats when compared to the STZ induced  diabetic rats. Higher doses of Karala (500 and 750mg/kg) are as effective as standard GLM dose on measured variables. This study demonstrated that Karala has hyperglycemia and antihyperlipidemic effect against STZ induced diabetic rats. These findings open the possibility of using Karala extract to treat diabetic animal and human patients although further research is warranted. DOI: http://dx.doi.org/10.3329/jesnr.v5i1.11550 J. Environ. Sci. & Natural Resources, 5(1): 29 - 37, 2012  

scholarly journals HEPATOPROTECTIVE AND NEPHROPROTECTIVE EFFECTS OF THE AQUEOUS EXTRACT OF TURMERIC (CURCUMA LONGA) IN RIFAMPICIN AND ISONIAZID-INDUCED HEPATOTOXICITY AND NEPHROTOXICITY IN RATS

2019 ◽  
pp. 293-298
Author(s):  
MAHDI M THUAWAINI ◽  
MAWAHIB B GASIM AL-FARHAAN ◽  
KARIMA F ABBAS
Keyword(s):  
Aqueous Extract ◽  
Total Bilirubin ◽  
Curcuma Longa ◽  
Albino Rats ◽  
Histopathological Study ◽  
Protective Effects ◽  
Significant Elevation ◽  
Liver And Kidney ◽  
Histopathological Studies ◽  
Kidney Functions

Objectives: The present study was designed to estimate the influences of oral administration of aqueous extract of turmeric (Curcuma longa) in hepatotoxicity and nephrotoxicity induced in rats by isoniazid and rifampicin (RIF) for 4 weeks. Influences were determined through the estimation of liver and kidney functions and histopathological changes. Materials and Methods: A total of 48 male albino rats were randomly divided into six groups: Normal control, INH+RIF treated rats, Turmeric aqueous extract 100 mg/kg treated rats, Turmeric aqueous extract 100 mg/kg + INH and RIF treated rats, Turmeric aqueous extract 200 mg/kg treated rats, Turmeric aqueous extract 200 mg/kg+ INH and RIF treated rats. Turmeric aqueous extract and INH + RIF (50 mg/kg bwpo, daily) were given for 4 weeks. Liver and kidney function markers (aspartate transaminase [AST], alanine transaminase [ALT], alanine phosphatase [ALP], bilirubin, blood urea, and creatinine) were determined enzymatically. In addition, tissues of liver and kidney were quickly separated and fixed in 10% formalin and subjected to histopathological studies. Statistical analysis was carried out using t-test. Results: The aqueous extract of turmeric (at a dose of 100 and 200 mg/kg bw, p.o. daily ) showed hepato- and reno-protective effects in hepato- and reno- toxicity induced by RIF and INH in rats. Significant elevation of serum ALT, AST, ALP, total bilirubin, creatinine, urea, and total protein, due to RIF and INH treatment, were significantly decreased. The histopathological study further confirmed the biochemical results. Conclusion: Results of the present study indicated that turmeric has hepatoprotective and renoprotective action against RIF- and INH-induced hepatic and renal injury in rats.

Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats

2017 ◽  
Vol 28 (4) ◽  
Author(s):  
Anthoneth Ndidi Ezejiofor ◽  
Zelinjo Nkeiruka Igweze ◽  
Nnaemeka Arinze Udowelle ◽  
Orish Ebere Orisakwe
Keyword(s):  
Alkaline Phosphatase ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Diabetic Rats ◽  
Control Group ◽  
Marker Enzymes ◽  
Hypoglycaemic Effect ◽  
Stem Extract ◽  
Costus Afer

AbstractBackground:In continuation of our study of the biological activity of the aqueous extract ofMethods:Thirty male albino Wistar rats (150−200 g) were weight-matched into six groups of five rats each. Groups 1 and 2 were the normal (non-induced) and toxic (alloxan-induced) controls, respectively. Groups 3−6 were induced and treated with 1, 2 and 3 g/kg of the aqueous extract ofResults:There was a significant reduction in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin triglyceride, creatinine, urea and electrolyte when compared with toxic control group. The liver marker enzymes were restored in all the treated groups. The aqueous stem extract possessed hypoglycaemic effect and reversed the histopathological damage in alloxan-induced diabetic rats.Conclusions:This study suggests that aqueous stem extract of

scholarly journals Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Mohammed A. Alsieni ◽  
Haddad A. El Rabey ◽  
Abdulbasit I. Al-Sieni ◽  
Madeha N. Al-Seeni
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Density Lipoprotein ◽  
Negative Control ◽  
Seed Extract ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Leaf Aqueous Extract

This study is aimed at comparing the antidiabetic and antioxidant potential of fenugreek and buckthorn which are commonly used in modulating diabetes in the Middle East. In this study, the antioxidant and antidiabetic activity of the aqueous extracts of the leaf and seed of fenugreek and buckthorn was tested in streptozotocin-induced diabetic male rats fed with a fat-rich diet for 8 weeks. Thirty-six male albino rats were divided into 6 groups ( n = 6 ); the 1st group was the negative control. Diabetes was induced in the other 30 rats using streptozotocin, which were then divided into 5 groups; the 2nd was the untreated positive diabetic group, the 3rd was treated with fenugreek leaf aqueous extract, the 4th was treated with the fenugreek seed aqueous extract, the 5th was treated with buckthorn leaf aqueous extract, and the 6th was treated with buckthorn seed aqueous extract. The positive control group showed an increase in blood sugar, glycated hemoglobin, liver function enzymes, lactate dehydrogenase, kidney indices, total cholesterol, triglycerides, low- and very-low-density lipoprotein, immunoglobulins, and lipid peroxidation and a decrease in high-density lipoprotein, albumin, and antioxidant activity. The histology of the liver and testes showed severe histopathological alterations. Rats of groups 4-6 that were treated with the aqueous extract of the leaf and seed extract of fenugreek and buckthorn showed improvement of all biochemical and histopathological parameters. The seed extract of fenugreek and buckthorn showed more antioxidant activity than their leaves.

scholarly journals DIABETIC NEPHROPATHY

2018 ◽  
Vol 25 (07) ◽  
pp. 1117-1123
Author(s):  
Faiza Irshad ◽  
Rabia Sajjad Toor ◽  
Madiha Hussain
Keyword(s):  
Body Weight ◽  
Diabetic Nephropathy ◽  
Serum Creatinine ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Kidney Weight ◽  
Experimental Group

Background: Zingiber Officinale Roscoe (Zingiberaceae family) is knownas Ginger. It is famous for its antioxidant properties. Objectives: To evaluate the effects ofGinger aqueous extract on the serum creatinine and paired kidney weight in Alloxan induceddiabetic nephropathy of albino rats. Study Design: Experimental study. Period: 06 months01-01-2013 to 30 June 2013. Setting: Anatomy Department, Sheikh Zayed, PGMI Lahore.Materials and Methods: Diabetes mellitus was induced with Alloxan intraperitoneally (150 mg/kg body weight) in Experimental groups B & C. Then the rats of experimental group C received200mg/kg body weight of ginger aqueous extract by gavage daily for five weeks starting from8th day after Alloxan injection. Results: Serum creatinine levels increased more in experimentalgroup B as compared to experimental group C. Group wise comparison of creatinine levelrevealed that the difference among control (A group) and experimental (B & C Groups) wassignificant having p-value <0.001. We observed that Paired kidney weight in experimentalgroup B increased as compared to control group A. Less increase in the paired kidney weightwas observed in experimental group C as compared to experimental group B. The differenceof mean paired kidney weight among three groups was significant having p-value <0.001.Conclusion: The results of the present study indicated that the co-treatment of Ginger aqueousextract prevented alloxan induced diabetic nephropathy in albino rats. The aqueous extract ofGinger showed amazing results on paired kidney weight.

scholarly journals Hepatoprotective effect of aqueous extract of Melothria perpusilla against carbon tetrachloride induced liver injury in albino rats

2017 ◽  
Vol 5 (3) ◽  
pp. 806 ◽  
Author(s):  
Nokul S. Yengkhom ◽  
Ngangom Gunindro ◽  
Sania M. Kholi ◽  
Rakesh S. Moirangthem ◽  
Bharati D. Rajkumari
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Aqueous Extract ◽  
Liquid Paraffin ◽  
Herbal Medicines ◽  
Modern Medicine ◽  
Hepatoprotective Effect ◽  
Albino Rats ◽  
Group Iii ◽  
Group I

Background: In absence of reliable hepatoprotective drug in modern medicine, the traditional herbal medicines have been emphasized. Present study was designed to assess hepatoprotective effect of aqueous extract of Melothria perpusilla (AEMP) against carbon tetrachloride (CCl4) induced liver injury.Methods: Five groups of animals with 6 rats in each were treated for 7 days.  Group I received 1% gum acacia in distilled water (1 ml/200 g p.o.) daily.  Group II, III, IV and V received CCl4 in liquid paraffin (1 ml/kg s.c.) on day 2, 4 and 6. Group III, IV and V were treated respectively with silymarin (100 mg/kg p.o.), AEMP- 200 and 400 mg/kg p.o. daily. On day 8, liver injury was assessed by measuring serum ALT, AST, ALP and bilirubin.Results: ALT, AST, ALP and bilirubin were significantly reduced in groups receiving both CCl4 and AEMP when compared with CCl4 treated group.Conclusions: AEMP produced hepatoprotective effect against CCl4 induced liver injury.

Chemical composition analysis and in vivoAnti-diabetic activity of aqueous extract of aerial part of Pallenis spinosa in diabetic rats

2021 ◽  
Vol 17 ◽  
Author(s):  
Abdelkrim Khettaf ◽  
Seloua Dridi
Keyword(s):  
Body Weight ◽  
Chemical Analysis ◽  
Aqueous Extract ◽  
Aerial Part ◽  
Control Group ◽  
Albino Rats ◽  
Spectrometric Analysis ◽  
Composition Analysis ◽  
Significant Activity

Background: The aim of this work is to confirm the anti-diabetic activity of Pallenis spinosa as recommended in traditional medicine and the chemical analysis of the aerial part of Pallenis spinosa. Methods: The isolated compounds have been identified by the chemical methods and spectrometric analysis such as: UV, 1H NMR, and 13C NMR spectroscopy. With regard to anti-diabetic activity, a series of experiments have been carried out in vivo on Westar albino rats. Diabetes has been induced in the animals by an intraperitoneal injection of streptozotocine; they have been treated a day with an aqueous extract from the aerial part infusion (250 and 500 mg/kg body weight) and glibenclamide (5 mg/kg body weight) for 21 days. Results: The chemical analysis of the aerial part of Pallenis spinosa has led to the isolation of five known flavanoid: Patuletin 7-galactopyranoside, patuletin-3-O-α-L-rharnnopyranosyl (1-6)-β-D-galactopyranoside, tricin 7-glucopyranoside, tricin and quercetin. The aqueous extract of both doses 250 mg and 500 mg has shown a significant activity in reducing blood sugar with 43.38% for the dose of 250 mg / kg and 37.76% for the dose of 500 mg / kg, as well as a significant decrease in the total fatness, triglycerides, and the total cholesterol levels in animals Treatment compared to diabetes control group (P = 0.05). We’ve used Glibenclamide as a reference, and it has shown similar results. Conclusion: In order to explore and develop new anti-diabetes drugs, more studies are needed on this plant for scrutinizing its mechanism of activity.

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