scholarly journals Morphological Characteristics and Pleasant Relationship Between Crossing of Black Rice Accesion with Inpara 5 that Containing SUB-1 Genes

2021 ◽  
Vol 7 (1) ◽  
pp. 39-43
Author(s):  
Utary Gladysha ◽  
E.S Halimi ◽  
Mery Hasmeda ◽  
Upit Sarimana

In South Sumatra the cultivation of black rice is uncommon. This is because of the lack of available land for planting which can be used to grow local black rice. Since more farmers dominate the white rice or brown rice cultivation. One effort that can be made to increase the area of local black rice planting areas is to use South Sumatra's vast swampy land. Related to the previous study, this research will be carried out by testing the growth of F1 seeds crossed by accession to high productivity local black rice and nutrition with marinated resistant Inpara 5 varieties. In quantitative parameters, the maximum average value of it was shown at the age of 12 MST plants that IM.6 plants at 145.08 as the highest average value of the crossing results. IM.6 was the highest median value with 144.5. The maximum standard deviation value was 5.06 for IT.11 plants, and the minimum value being 141.4 for IM.6 forms and 150.0.6 for IM.6 plants. The qualitative parameters consist of plant shape, stem color, leaf color, and flag leaf are seen when the plant is 6 MST or in the vegetative phase. Done by describing plants from 5 sample plants per randomly selected population. From the result of this research, there is no significant differential between accession and parental.

2020 ◽  
Vol 15 (3) ◽  
pp. 159
Author(s):  
Annis Catur Adi ◽  
Mahmud Aditya Rifqi ◽  
Merryana Adriani ◽  
Farapti Farapti ◽  
Nila Reswari Haryana ◽  
...  

Rice is the staple food of Indonesian which has superior taste which made it rooted in the eating culture in Indonesia. Nowadays, there is still limited research comparing cooking methods in various rice varieties. This study aim to analyze the eff ect of various cooking methods and rice variety on sensory quality. There were two cooking methods tested, traditional method that combines boiling and steaming, and rice cooker steaming method. Sensory test (quality characteristic) and acceptance test were measured using hedonic scale on 30 semi-trained panelist to evaluate the eff ect of cooking method on four rice variety, that are white rice Berlian Sae, white rice Super Slyp, brown rice, and black rice. Statistical test used was ANOVA and continued with Duncan test. Results showed diff erences in ratio of water and cooking time of various types of rice with diff erent methods. White rice (Slyp Super) with conventional cooking methods was more acceptable to panelist with the characteristics of bright colors, smell, neutral taste, and delicate texture. There was a signifi cant diff erence in panelist acceptance based on type of rice (p<0.05) which brown rice and black rice had lower acceptability than white rice. There was no signifi cant diff erence in acceptance by cooking method (p>0.05), but there was a tendency that traditional method has higher acceptability. Color indicator became main indicator for panelist determined overall acceptability, so that it can be considered in the development of rice cooking methods.


2007 ◽  
Vol 77 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Kim ◽  
Kim ◽  
Lee ◽  
Kim ◽  
Lee

This study was designed to investigate the influence of natural brown and black rice consumption on plasma lipid parameters, transit time, and thickness of the aortic wall in rats fed different combinations of grains. Fifty male rats were divided into five groups and raised for eight weeks with diets containing white rice (WR), white rice and brown rice (WRBR), white rice and black rice (WRBL), brown rice and black rice (BRBL), or wheat flour (WH). Gut transit time was the shortest in the BRBL group, and was longer in the order of WRBL, WRBR, WR, and WH group. Plasma lipid profiles differed significantly according to the grain combination. The BRBL group had the lowest levels of triglyceride (TG), total cholesterol (T-C), and low-density lipoprotein cholesterol (LDL-C), and the highest plasma high-density lipoprotein cholesterol (HDL-C) concentration among all groups. The aortic wall thickness hatro for body weight was lower in rats in order of BRBL, WRBR, WH, WRBL, and WR group. In conclusion, black rice and brown rice have anti-atherogenic effects by decreasing plasma lipide profiles and attenuating the thickness of the aortic wall.


2016 ◽  
Vol 35 (2) ◽  
pp. 119 ◽  
Author(s):  
Kristamtini Kristamtini ◽  
Sutarno Sutarno ◽  
Endang Wisnu Wiranti ◽  
Setyorini Widyayanti

Black rice is a local rice variety with some advantages and weaknesses. The desirable character of black rice is its high anthocyanin content, while the weaknesses are late maturing, tall plant stature and low grain yield. Crosses were made between two parents aimed to recombine the superior traits. The effectiveness and efficiency of selection would be determined by the magnitude of the heritability of traits and the selection progress. This study was aimed to determine the heritability estimates in a broad sense and the genetic advance of agronomic characters of F2 generation, from crosses of local black rice and high yielding variety of white rice. The experiment was conducted in Padasan, Pakembinangun, Pakem, Sleman, Yogyakarta from January to May 2013. The F2 population derived from crosses of black rice x white rice, with the morphological traits of hairless black rice (S) crossed with Situbagendit (G) white rice, Cempo ireng (C) with Situbagendit (G) and Cempo ireng (C) with Inpari 6 (I). The F2 plants population were planted without replication. Genetic parameters estimated were calculated for broad sense heritability and genetic advance from selection. Results showed that the F2 population had high broad sense heritability estimates for all characters observed, including: plant height, flag leaf length, number of productive tillers, panicle length, number of filled grains per panicle, number of empty grains per panicle, maturity and rice color, in the three F2 populations obtained from crosses of S x G, C x G and C x I. Only the plant height of S x G cross had a medium broad sense heritability estimate. The large heritability estimates indicated that the respective character was controlled by genetic factor more than environmental ones. High genetic advance would be obtained in the flag leaf length, number of productive tillers, panicle length, number of filled grains/panicle, number of empty grains/panicle and rice color from S x G, C x G and C x I crosses. Genetic advance of plant height was medium (at S x G and C x I crosses) to high (C x G crosses). The maturity trait had low genetic advance on cross S x G; C x G; and Cx I. The high value of genetic advance of character would suggest that selection on the character’s appearance would be successful.


2019 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Daniel Akbar Wibowo ◽  
Dini Nurbaeti Zen ◽  
Yalis Agustina

Disease that is often complained by the public today one of the pain in the bone, which is better known by the community with rheumatism. Rheumatism or rheumatoid arthritis is a systemic, progressive, chronic and tendonic inflammatory disease of joints and connective tissue symmetrically. One way of management of rheumatoid arthritis pain is back massage therapy. Back Massage is one of the techniques to give massage action on the back with lotions/balm for 10-15 minutes, the warm sensation leads to vasodilation of blood vessels that will improve blood circulation in the area so that the activity of the cell is increased and will reduce pain, increase comfort, reduce muscle tension and improve physical and psychological relaxation. This study aims to determine the effect of Back massage therapy to decrease the pain level of rheumatoid arthritis patients in Rajadesa Village Rajadesa Sub District Ciamis District in 2018. Type of research using Quasi Experiment Design with one group pretest-posttest design. Total sample 48 respondents with total sampling technique. Data collection using experimental method with VDS (Verbal Descriptor Scale) pain rate measurement tool. The result of statistical test by using Linear Regression shows Sig = 0,000 <0,05, t value = 18,935> 2,012. Then Ha is accepted, and the average value before therapy is 3.27 with a standard deviation of 0.818, whereas after therapy is 2.23 with a standard deviation of 0.881, meaning there is the effect of back massage therapy on the decrease of pain level in patients with rheumatoid arthritis. So to reduce pain in patients with rheumatoid arthritis can be given back massage therapy.


Author(s):  
Wirda Linda

This research is motivated by the low desire of students in writing travel reports. The lack of students' knowledge of the report concept, the lack of students' knowledge of the 5W + 1H report points of good and correct language, the lack of students' knowledge of the spatial, time and topic pattern and not yet reached KKM 75. The method used by the teacher has not been interesting, lecture method. The purpose of this study is to describe the skills of writing travel reports by using Round Club learning model which is viewed from the aspect of understanding the report concept, the use of 5W +1H report points, the spatial, time, and topic pattern.The population of this study is the students of class V Lessons Year 2017/2018 which amounted to 2 classes with the number 80. The sample of research as much as two classes taken by the sample of propotional.Class V.1 as experimental class and class V.2 as control class. The research instrument used is performance test. Provide an assessment by specifying the subject of the 5W + 1H report, as well as the spatial, time and topic pattern. Data were analyzed by 't' test by first testing normality, homogeneity, and hypothesis testing.The results showed that the average control class 68 with more than enough qualifications with standard deviation 16.96. 83 experimental class with good qualification and standard deviation of 15.42 and there is a significant influence on the result of writing skill of class V SDN 01 Nagari Bukik SikumpaSubdistrict, Lima Puluh Kota. This is evidenced by the average value of writing skills in the experiment class higher than the average value in the control class. Normality test results indicate that the two sample classes of  Lo  values in the control class -0.2141 are smaller than the normal 0.190 Lt distributed. Homogeneity test results that the variation of this study is homogeneous at a real level of 0.05, because Ftable 2.16 > Fhitung 1.21 and the results of data analysis then obtained = 2.78 > 1.70 t table, so H0 rejected and H1 accepted. It can be concluded that there is Influence. Using  Learning  Model of Student Group Writing  Skills Travel Report of students of class V SDN 01 Nagari Bukik Sikumpa Subdistrict, Kabupaten Lima Puluh Kota.KeyWords: model pembelajaran round club, menulis laporan perjalanan.


Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 685
Author(s):  
Enerand Mackon ◽  
Yafei Ma ◽  
Guibeline Charlie Jeazet Dongho Epse Mackon ◽  
Qiufeng Li ◽  
Qiong Zhou ◽  
...  

Anthocyanins belong to the group of flavonoid compounds broadly distributed in plant species responsible for attractive colors. In black rice (Oryza sativa L.), they are present in the stems, leaves, stigmas, and caryopsis. However, there is still no scientific evidence supporting the existence of compartmentalization and trafficking of anthocyanin inside the cells. In the current study, we took advantage of autofluorescence with anthocyanin’s unique excitation/emission properties to elucidate the subcellular localization of anthocyanin and report on the in planta characterization of anthocyanin prevacuolar vesicles (APV) and anthocyanic vacuolar inclusion (AVI) structure. Protoplasts were isolated from the stigma of black and brown rice and imaging using a confocal microscope. Our result showed the fluorescence displaying magenta color in purple stigma and no fluorescence in white stigma when excitation was provided by a helium–neon 552 nm and emission long pass 610–670 nm laser. The fluorescence was distributed throughout the cell, mainly in the central vacuole. Fluorescent images revealed two pools of anthocyanin inside the cells. The diffuse pools were largely found inside the vacuole lumen, while the body structures could be observed mostly inside the cytoplasm (APV) and slightly inside the vacuole (AVI) with different shapes, sizes, and color intensity. Based on their sizes, AVI could be grouped into small (Ф < 0.5 um), middle (Ф between 0.5 and 1 um), and large size (Ф > 1 um). Together, these results provided evidence about the sequestration and trafficking of anthocyanin from the cytoplasm to the central vacuole and the existence of different transport mechanisms of anthocyanin. Our results suggest that stigma cells are an excellent system for in vivo studying of anthocyanin in rice and provide a good foundation for understanding anthocyanin metabolism in plants, sequestration, and trafficking in black rice.


2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Romaya Sitha Silitonga ◽  
Jumaria Nasution

ABSTRACT Black rice is very potential to be expanded because the content of anthocyanin, vitamin B1, and vitamin E black rice is higher than brown rice or white rice. At present, black rice producers are in Java, whereas in Sumatra, South Tapanuli District, Angkola Sub-District is a new type of black rice for rice farmers, so it is necessary to introduce and cultivate. The aims of the research was to determine the effect of chicken manure and paclobutrazol concentration on the tiller number and stems height of the rice plant in Batang Angkola District, South Tapanuli. This research method used was Randomized Block Design Factorial with two factors. The first factor was application of chicken manure (C) with dosage 0 g/pot 3 g/pot (C1), 6 g/pot (C2) and 9 g/pot (P3). The second factor was application paklobutrazol (P) with concentrations of 0 ppm (P0), 25 ppm (P1), 50 ppm (P2) and 75 ppm (P3). Statistical analysis used Anova 5%. If there is a difference followed by DMRT. The result showed that application of chicken manure at 9 g/pot (C3) and paclobutrazol 75 ppm (P3) were produce more tiller number and able to decrease height of black rice plant. Key words : Black Rice, Chicken manure, Paklobutrazol, South Tapanuli District, Tiller number, ABSTRAK Padi hitam sangat potensial dikembangkan karena kandungan antosianin, vitamin B1, dan vitamin E padi hitam lebih tinggi dari beras merah maupun beras putih. Adapun daerah penghasil beras hitam saat ini masih terdapat di daerah-daerah Pulau Jawa, sementara di Pulau Sumatera, khususnya di Kabupaten Tapanuli Selatan, Kecamatan Batang Angkola beras hitam masih tergolong tanaman padi jenis baru bagi para petani beras sehingga perlu dilakukan upaya perkenalan dan budidaya di daerah ini. Penelitian ini bertujuan mengetahui pengaruh konsentrasi kotoran ayam dan paclobutrazol terhadap jumlah anakan dan tinggi batang padi hitam yang tumbuh di Kecamatan Batang Angkola, Tapanuli Selatan. Metode penelitian yang digunakan adalah Rancangan Acak Kelompok Faktorial dengan 2 faktor. Faktor pertama, aplikasi Kotoran ayam (C) dengan dosis 0 g/pot (C0), 3 g/pot (C1), 6 g/pot (C2) dan 9 g/pot. Faktor kedua, paklobutrazol (P) dengan konsentrasi 0 ppm (P0), 25 ppm (P1), 50 ppm (P2) dan 75 ppm (P3). Data dianalisis menggunakan statistik analisis variansi pada taraf kesalahan 5%. Apabila ada beda nyata dilanjutkan dengan DMRT. Hasil penelitian aplikasi Kotoran ayam 9 g/pot (C3) dan paclobutrazol 75 ppm (P3) menghasilkan jumlah anakan lebih banyak dan dapat menekan pertumbuhan tinggi batang padi hitam yang ditanam. Kata Kunci : Jumlah anakan, Kabupaten Tapanuli Selatan, Kotoran Ayam, Paclobutrazol, Padi hitam.


2018 ◽  
Vol 1 (1) ◽  
pp. 009-020
Author(s):  
Sunartih Sunartih ◽  
Marungkil Pasaribu ◽  
Amiruddin Hatibe

This study aims to determine whether there is an influence of ASSURE learning model on student learning outcomes in temperature and heat material of class XI SMA. The method used is quasi-experimental with equivalent pretest-posttest design. The population of this study were all students of class XI SMA . Sampling was carried out by purposive sampling with the sample of the study being class XI Mipa 2 as the experimental class and class X1 Mipa 5 as the control class. The research instrument in the form of learning outcomes tests and observation sheets that have been validated by the validator and field tested. Data analysis used inferential statistics is normality, homogeneity, hypothesis testing (2-party t test). Based on the results of research and analysis of research data, obtained the value of student learning outcomes at posttest average value of the experimental class is 14.90 with a standard deviation of 3.23 and for the control class of 11.57 with a standard deviation of 2.99. The test results of the t test statistic of 2 parties from hypothesis testing obtained the price thitung(4,11)>ttabel(1,67) or thitung(-4,11)>ttabel(-1,67) so that H1 is accepted and H0 is rejected. This result states that there are differences in student learning outcomes in physics subjects between classes taught with the ASSURE learning model and Direct Intruction learning models. It can be concluded that there is an influence of the ASSURE learning model on student learning outcomes in temperature and heat material in class XI of SMA. Keywords: assure learning model, learning outcomes, temperature and heat  


Plant Disease ◽  
2021 ◽  
Author(s):  
Danilo Neves ◽  
Bill Bruening ◽  
Carrie A Knott ◽  
Chad Lee ◽  
Carl Bradley

The Kentucky distilling industry ranks as one of the state’s largest industries and continues to expand. In 2017, the Kentucky distilling industry was responsible for approximately $235 million in state and local tax revenues (Coomes and Kornstein, 2019). Rye (Secale cereale L.) grains are a vital component for production of some distilled spirits. Although winter rye is produced on relatively few hectares in Kentucky currently, a recent initiative has supported expanding production to help meet the growing demand of local distilleries. University of Kentucky winter rye research field trials were visited in Caldwell and Logan Counties, KY in May 2018, and in Fayette County, KY in May 2019. Leaves were collected that had dark brown, oval to irregular-shaped lesions with definite margins and yellow halos. Symptoms were present on approximately 50% to 80% of the flag leaves, with severity ranging from 5% to 30% of the flag leaf area affected. Leaves were surface-disinfested by soaking in a 2% NaOCl solution for 1 min and rinsed twice in sterilized water and then placed in a humidity chamber (plastic bag with moist paper towels) at room temperature (approximately 24°C) to induce fungal sporulation. Seventeen single-spore isolates were obtained and stored at -80°C in 15% glycerol solution. Isolates were grown on potato dextrose agar under 12 h cycles of white light/darkness for 5 days. Colonies were gray to black. Conidia that formed were mostly straight or slightly curved, dark olivaceous brown, 3-7 septate, and 41.0-90.4 × 15.2-29.3 µm. Based on the symptoms observed on the collected leaves and these morphological characteristics similar to those described by Chang and Hwang (2000) and Manamgoda et al. (2014), the fungus was tentatively identified as Bipolaris sorokiniana (Sorokin) Shoemaker. The sequence of internal transcribed spacer regions (ITS) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used to identify three isolates (18Bs004, 18Bs111 and 19Bs064) using primer ITS1/ITS4 (White et al. 1990) and GPD1/GPD2 (Berbee et al. 1999), respectively. The sequences were deposited in GenBank with accession numbers MT457817, MT457818 and MZ066635 for ITS sequences and MZ073644 to MZ073646 for GAPDH sequences. BLAST searches with ITS and GAPDH sequences matched 100% identity (344/344 bp and 515/515 bp for ITS and GAPDH sequences, respectively) to B. sorokiniana (GenBank accession No. MT254731 and MH844813, respectively). To prove pathogenicity, a conidial suspension (1 × 105 conidia/ml) was used to inoculate 15-day-old cultivar ‘Serafino’ winter rye plants in the greenhouse. Leaves of 8 plants were inoculated with 50 ml of the conidial suspension using a spray bottle. Plants were covered with a transparent plastic bag for 48 h, and symptoms were observed 10 days after inoculation. Leaf lesions, similar to those described above, were present on all inoculated plants, but no symptoms were observed on non-inoculated control plants. Bipolaris sorokiniana was reisolated from symptomatic leaves and the identity of the pathogen was confirmed based on the morphology previously described. To our knowledge, this is the first report of spot blotch caused by B. sorokiniana on winter rye in Kentucky, but B. sorokiniana has been reported on rye in the neighboring state of Virginia (Roane 2009). Kentucky produces approximately 150,000 and 4,000 ha of winter wheat (Triticum aestivum) and winter barley (Hordeum vulgare) annually, respectively, which are both known hosts of B. sorokiniana (Kumar et al. 2002). An isolate of B. sorokiniana from rye was reported by Ghazvini and Tekauz (2007) to be less virulent on barley differential lines. Further research is needed to better understand spot blotch distribution, susceptibility in winter rye cultivars, and potential yield and quality loss implications in winter rye production and end use. It is unknown how susceptible various winter rye cultivars grown in Kentucky are to spot blotch.


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