scholarly journals Study of the Antibacterial Activity of Elettaria Cardamomum Extracts on the Growth of Some Gingivitis Inducing Bacteria in Culture Media

2020 ◽  
Vol 10 (01) ◽  
pp. 38-45
Author(s):  
Ali M. Ghazi ◽  
Ahmed J. Na’ma ◽  
Qassim H. Kshash ◽  
Nafae S. Jasim
Keyword(s):  
Pathogenic Bacteria ◽  
Culture Media ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Significant Activity ◽  
Antibacterial Screening ◽  
Zones Of Inhibition ◽  
Inhibition Zones ◽  
Almost All ◽  
Phytochemical Profiles

The our work was carried out with an objective to appraisal the antibacterial action of cardamom extracted by four different solvents and prepared in a number of concentrations (50, 100 and 200 mg/mL) toward three different pathogenic bacteria responsible mainly for induce gingivitis infection and comparison their action with the action of antibiotics (Ciprofloxacin (5μg), Ampicillin (30μg) in culture media. The results of phytochemical profiles for different extracts of cardamom showed the presence of alkaloids, glycosides, tannins, and terpenes in all used extracts while flavonoids were present in all extracts except watery extract. Saponins were present only in the ethanolic extract, while phenols were found to be present only in ethanolic and chloroformic extracts. The antibacterial screening of the different extracts of cardamom and standard antibiotics showed various degrees of zones of inhibition in the culture media depending largely upon the type of plant extract, the concentration of extract in addition to the type of tested bacterial. Almost all the cardamom extracts were found to have significant activity (p less than 0.05) against all tested bacteria com the pared with a negative control. The highest antibacterial potential was observed for the ethanolic cardamom extract, whereas other cardamom extracts showed closed results in general. At the same time, the current study was recorded that inhibition zones diameter against tested bacteria raised significantly (p less than 0.05) as the extract concentration raised. The MIC values of watery, ethanolic, chloroformic, and acetonic extracts of cardamom ranged from 0.624 to 1.248 mg/mL, 0.078 to 0.312mg/mL, 0.624 to 1.248 and 0.312 to 0.624 mg/mL against tested bacteria respectively. While the MBC values ranged from 1.248 to 10 mg/mL, 0.624 to 2.5 mg/mL, 2.5mg/mL to 5 mg/mL and 2.5mg/mL respectively.

scholarly journals Study of the antibacterial activity of Nigella sativa ethanol extraction the growth of Staphylococcus aureus in culture media

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Mohammed AbdulReda Yassen
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Culture Media ◽  
Nigella Sativa ◽  
Inhibition Zone ◽  
Negative Control ◽  
Significant Activity ◽  
Extract Concentration ◽  
Zones Of Inhibition

Bacterial infections become a great health problems. The new antimicrobial agents are will reduce this problem. In this study, antimicrobial of activity of extract of Nigella sativa seed that used against Staphylococcus aureus isolates was evaluated. Started with an objective to appraisal the antibacterial potentials of ethanol extractofNigella sativaprepared in different concentrations (50,100, 200& 400 mg/ml) against Staphylococcus aureus and associated its activity with antibiotic concentration value Ciprofloxacin (5µg),Amoxicillin (25µg) & Penicillin (6µg) by determining the inhibition zone produced around the holes after growth on Muller-Hinton agar. This results showed activity of antibacterial of the Nigella sativa at differentconcentrationsand standard antibiotics exhibited various degrees of zones of inhibition in the culture media depending mainly upon the type of plant extract,concentration of extract in addition to the type of tested bacterial. Nearly all the Nigella sativa extracts were found to have significant activity (p<0.05) against all tested bacteria related with negative control. In the same time the current study was recorded that inhibition zones diameter against examined bacteria raised significantly difference (p<0.05) at extract concentration raised (400 mg / ml ). The MIC values ofethanolic extracts of Nigella sativaextended from 0.156 to 0.312 mg/ml against tested bacteria. Whereas the MBC values ranged 1.248 mg/ml.

scholarly journals Phytochemical and Antimicrobial Properties of Leaves ofAlchonea Cordifolia

2010 ◽  
Vol 7 (3) ◽  
pp. 1071-1079 ◽  
Author(s):  
N. J. George ◽  
I. B. Obot ◽  
A. N. Ikot ◽  
A. E. Akpan ◽  
N. O. Obi-Egbedi
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Zone Of Inhibition ◽  
Bioactive Substances ◽  
Butanol Fraction ◽  
Zones Of Inhibition

The phytochemistry ofAchornea cordifolialeaf extract using different solvents was studied using standard methods. The effects of the leaf extract on some pathogenic bacteria and fungi were also examined. The Phytochemical screening of the leaves shows the presence of useful ethno-botanical bioactive substances such as tannin, saponin, flavonoid, cardiac glycoside and anthraquinone, while alkaloid, phlobatanin and terpene also tested for were absent. The butanol fraction of the extract gives the highest zone of inhibition (13.0 mm) againstStaphylococcus aureus, Escherichia coliandpseudomonas aeruginosa, which are in parity. The control, gentamycin injection gives 12.5 mm, 11.0 mm and 12.0 mm respective zones of inhibition against theStaphylococcus aureus, Eschariclia coliandPseudomonas aeruginosa. Also, the butanol fraction of the extract shows highest zone of inhibition of 17.0 mm againstCandida albican, while ethanolic extract gives 13.0 mm zone of inhibition againstTrichophyton violaceum.

scholarly journals Phytochemical Screening and Antibacterial Activities of Senggani (Melastoma malabathricum L.) Ethanolic Extract Leaves

2021 ◽  
Vol 26 (4) ◽  
pp. 562-568
Author(s):  
Retno Widowati ◽  
Sri Handayani ◽  
Ahmad Ridho Al Fikri
Keyword(s):  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Active Compounds ◽  
Bacteria Growth ◽  
Significant Difference

The aim of the current research was to perform phytochemical screening and to know the pathogenic antibacterial activities of senggani leaves extract. Phytochemical screening was done by testing eight active compounds. Antibacterial activities testing was done by using well diffusion method at concentration level of 25%, 50%, 75%, 100%. Positive control was amoxicillin, while the negative control was aquadest. Meanwhile, pathogenic bacteria were Escherichia coli, Shigella dysenteriae, Staphylococcus aureus, and Pseudomonas aeruginosa. Research design applied was complete random design, in which the data collected were analyzed using one-way ANOVA, continued by post-hoc test through Tukey method to know which concentration provide the most significant difference. Research results showed that ethanolic extract of senggani leaves has eight active compounds, those are phenolics, alkaloids, flavonoids, tannins, triterpenoids, glycosides, steroids, and saponins. This further proved that the ethanolic extract of senggani leaves have antibacterial activity and are able to inhibit the growth of all bacteria tested. The best ability shown to inhibit E. coli bacteria was at concentration of 100%, Sh. dysenteriae and P. aeruginosa started at the concentration of 75%, and S. aureus started at the concentration of 50%.   Keywords: active compounds, bacteria growth, inhibition, well diffusion

scholarly journals Antimicrobial activity and cytotoxicity of endophytic fungi associated with four medicinal plants from Sudan

2020 ◽  
Vol 9 (5) ◽  
pp. 348-355
Author(s):  
Ietidal E. Mohamed ◽  
◽  
Tawheeda A Abd Alati ◽  
◽  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Cell Line ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Spectroscopic Techniques ◽  
Significant Activity ◽  
Plant Host ◽  
African Green Monkey Kidney

In this study four medicinal plants, used in Sudan to cure different types of health problems, were selected for isolation of endophytic fungi, fungi reside within the plant host tissues without causing disease symptoms. A total of 20 distinct endophytic fungi species were isolated from surface- sterilized parts of the plants, Kigelia africana, Cymbopogon proximus, Balanites egyptiaca and Solenostemma argel. Nine of the isolated fungi were identified according to their microscopic and macroscopic characteristics. The remaining strains which did not have spores or have sterile mycelium were given codes as unidentified species (UID). The ethyl acetate extracts of the pure isolated endophytic fungi were prepared from fungal material and culture media. Antimicrobial activity using agar diffusion well against different pathogenic microbes at 500 µg/ml concentration and MTT cytotoxicity bioassay using African green monkey kidney normal cell line (vero cell line) were performed for all extracts. The extracts were then screened for the presence of Taxol, a diterpene anticancer drug, using different chromatographic and spectroscopic techniques. The antimicrobial activity against the most common pathogenic bacteria and fungi revealed significant activity with inhibition zone range between 14- 37 mm compare to antimicrobial controls 17- 26 mm at 40µg/ml. The cytotoxicity assay results showed that the IC50 values were quite diverse, ranging from 3.5 μg/ml to more than 1000 μg/ml, whereas that of the positive control podophyllotoxin, a wellknown cytotoxic lignan, was 2.72 μg/ml. Taxol was detected in reference to standard one from the extract of unidentified fungi No. 10 of Kigelia africana,. Future work will focus on the bioassay guidedfractionation of potent bioactive extracts to isolate the active compounds responsible for their activities. Improvement of culture techniques and the application of genetic engineering to increase taxol production will be carried out, followed by isolation of pure Taxol from the active crude extract.

scholarly journals Steroids from Marine-Derived Fungi: Evaluation of Antiproliferative and Antimicrobial Activities of Eburicol

Marine Drugs ◽  
2019 ◽  
Vol 17 (6) ◽  
pp. 372 ◽  
Author(s):  
Dos Santos Dias ◽  
Couzinet-Mossion ◽  
Ruiz ◽  
Lakhdar ◽  
Etahiri ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Cancer Cell Lines ◽  
Antimicrobial Activities ◽  
Significant Activity ◽  
Bioactive Lipids ◽  
Fungal Strains ◽  
Mcf 7

The most common sterol in fungi is ergosterol, which has frequently been investigated in human pathogenic fungal strains. This sterol, and others isolated from fungal strains, has also demonstrated cytotoxicity against cancer cell lines and antimicrobial activities. Marine fungi can produce high amounts of bioactive compounds. So, a screening was performed to study sterol composition using GC/MS in 19 marine fungal strains and ergosterol was always the major one. One strain, Clonostachys rosea MMS1090, was selected due to its high amount of eburicol and a one strain many compounds approach was performed on seven culture media to optimize its production. After purification and structural identification by NMR, eburicol was assessed against four cancer cell lines, MCF-7, MDA-MB-231, NSCLC-N6-L16 and A549, and seven human pathogenic bacteria Staphylococcus aureus, Bacillus sp., Bacillus cereus, Listeria ivanovii, Escherichia coli, Citrobacter freundii and Salmonella spp. The most significant activity was cytotoxicity against MCF-7 cells (2 µM). This is the first report of such an accumulation of eburicol in the marine fungal strain C. rosea confirming its potential in the production of bioactive lipids.

scholarly journals Formulation of Antiacne Cream Dosage Form Containing Mangosteen (Garcinia mangostana L.) Pericarp Ethanolic Extract

2018 ◽  
Vol 1 (1) ◽  
pp. 37-44
Author(s):  
Supomo ◽  
Anita Apriliana ◽  
Titin Purnawati ◽  
Ainur Risqi
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Dosage Form ◽  
Storage Time ◽  
Ph Value ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Inhibition Zones

Abstract. The aim of this study was to formulate ethanolic extract of mangosteen (Garcinia mangostana L.) pericarp (EEMP) in the antiacne cream dosage form. The formulae were made with 3 varied concentrations of 0.5%, 1.5% and 4.5%. The tests carried out in this study were  organoleptic cream preparations (odor, color, texture), homogeneity, pH, determination of the type of cream, spreadability, and the antibacterial activity against Staphylococcus epidermidis. The results of physical property evaluations showed that the EEMP indicated the pH value of 6, the spredability of the cream increased along with the increasing length of storage time. Duncan Test on the measurement of diameter dispersibility based on storage time showed that there were significant differences between the spreadability of the cream B and C. The cream produced was easy to wash indicating oil in water (o/w) type. The results of antibacterial activity test applying agar diffusion method using Mueller Hinton medium Agar (MHA) indicated that the inhibition zones of formulae II and III were 5.2 and 6 mm, respectively. Whereas, the formula I as well as the negative control showed no inhibition zones. It can be concluded that the EEMP can be formulated as an antiacne cream. The best cream formula was the formula II with the results of physical properties test were quite stable and able to produce inhibition zone against Staphylococcus epidermidis.   Keywords: Garcinia mangostana, cream, antiacne, antibacterial, Staphylococcus epidermidis.

scholarly journals In vitro antimicrobial activity of endodontic pastes with propolis extracts and calcium hydroxide: a preliminary study

2008 ◽  
Vol 19 (4) ◽  
pp. 301-305 ◽  
Author(s):  
Giovanna Pires da Silva Ribeiro de Rezende ◽  
Luciane Ribeiro de Rezende Sucasas da Costa ◽  
Fabiana Cristina Pimenta ◽  
Daniela Abrão Baroni
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Calcium Hydroxide ◽  
Propylene Glycol ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Control Group ◽  
Propolis Extract ◽  
Inhibition Zones

The purpose of this study was to evaluate the antimicrobial activity of two experimental pastes containing propolis extract associated with calcium hydroxide against polymicrobial cultures collected from 16 necrotic and fistulae root canals in primary molars of 4-8-year-old children of both sexes. The agar-well diffusion technique was used to determine the antimicrobial activity of the following pastes: 11.0% ethanolic extract of propolis (EEP) + calcium hydroxide; and 11.0% extract of propolis without ethanol (EP) + calcium hydroxide. EEP, EP and the association of calcium hydroxide and propylene glycol (CHP) was used as the positive control groups, and propylene glycol was used as a negative control group. Friedman and Wilcoxon tests were used to compare the data from the microbial growth inhibition zones (p<0.05). Paste 1 and Paste 2 showed larger growth inhibition zones against microorganisms from root canal samples than CHP (p=0.021 and 0.003, respectively). Paste 2 tended to have larger growth inhibition zones than Paste 1 (p=0.053). The association between propolis and calcium hydroxide was effective in controlling dental infections in vitro.

Susceptibility of animal pathogenic bacteria to an ethanolic extract from jambolan (Syzygium cumini) leaves

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
AL Magalhães ◽  
G Melo ◽  
N Gabriel ◽  
G Gabas ◽  
G Gonçalves ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Syzygium Cumini

The size of extracellular vesicles secreted by different types of stem cells

2018 ◽  
pp. 38-42
Author(s):  
И.Б. Алчинова ◽  
М.В. Полякова ◽  
И.Н. Сабурина ◽  
М.Ю. Карганов
Keyword(s):  
Stem Cells ◽  
Extracellular Vesicles ◽  
Culture Media ◽  
Living Organism ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Study Results ◽  
Multipotent Mesenchymal Stem Cells ◽  
Rat Adipose Tissue ◽  
Almost All

Механизм терапевтического действия мультипотентных мезенхимных стволовых клеток (ММСК) на облученный организм в последнее время вызывает повышенный интерес исследователей. В качестве активного участника паракринного механизма реализации этого эффекта предлагают рассматривать внеклеточные везикулы, секретируемые практически всеми клетками живого организма. Цель работы: выделить и охарактеризовать внеклеточные везикулы, продуцируемые стволовыми клетками различной природы. Материалы и методы. Суспензии внеклеточных везикул, выделенных по модифицированному протоколу дифференциального центрифугирования из культуральных жидкостей от культур ММСК костного мозга человека 2-го пассажа и ММСК жировой ткани крысы 4-го пассажа, были проанализированы методом просвечивающей электронной микроскопии и методом анализа траекторий наночастиц. Результаты. Исследование показало наличие в обоих образцах микрочастиц размерами до и около 100 нм, однако процентное содержание частиц разных размеров в суспензии различалось для двух анализируемых типов клеток. Заключение. Полученные результаты могут свидетельствовать о специфике секреции, обусловленной клеточным типом. A mechanism of the therapeutic effect of multipotent mesenchymal stem cells (MMSC) on irradiated body has recently arisen much interest of researchers. Extracellular vesicles (EVs) secreted by almost all cells of a living organism were suggested to actively contribute to the paracrine mechanism of this effect. The aim of the study was isolation and characterization of extracellular vesicles produced by various types of stem cells. Materials and methods. Suspensions of EVs were isolated from culture media of passage 2 human bone marrow-derived MMSC and passage 4 rat adipose tissue-derived MMSC using a modified protocol of differential centrifugation and then studied using transmission electron microscopy and nanoparticle tracking analysis. Results. The study showed the presence of microparticles with a size of >100 nm in the examined samples. However, the percent content of particles with different sizes in the suspension was different in two analyzed types of cell culture. Conclusion. The study results might reflect a specificity of secretion determined by the cell type.

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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