scholarly journals PHYSICS AND CHEMICAL, IMMUNOENZYME AND BIOCHEMICAL INDICATORS OF ORAL FLUID IN PERIODONTITIS IN PATIENTS WITH RHEUMATOID ARTHRITIS

2021 ◽  
pp. 15-20
Author(s):  
O. Syniachenko ◽  
M. Iermolaieva ◽  
S. Iarova ◽  
D. Gaviley ◽  
K. Liventsova ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oral Fluid ◽  
Cyclic Peptide ◽  
Pathological Process ◽  
Microbial Colonization ◽  
Control Group ◽  
Average Weight ◽  
Cytokine Network ◽  
Anaerobic Microorganisms ◽  
Gingival Mucosa

Infection with comorbid periodontitis due to transient bacteremia in patients with rheumatoid arthritis (RA) can lead to microbial colonization outside the mouth and increase systemic immune inflammation. Periodontitis-induced endotoxemia increases the sensitivity of RA patients to autoantigens by activating membrane proteins, pro-inflammatory enzymes, and cytokines. RA and periodontitis have similar osteoclasia, allelic genes and general imbalance in the state of the cytokine network, and carbamylation of proteins and the formation of extracellular traps of neutrophils are associated with simultaneous autoimmune imbalance in the combination of RA and periodontitis, but the listed pathogenetic mechanisms require further study. The purpose and objectives of the study: to study the properties of the oral fluid in periodontitis in patients with RA, to determine the clinical and pathogenetic significance of changes in physicochemical, enzyme immunoassay and biochemical parameters in the pathology of the oral cavity and joints, to compare the indicators with the nature of the gingival microbiota. Material and methods. The study included 173 RA patients aged 18-76 years (average 46 years), among whom there were 21% of men and 79% of women. The duration from the first manifestation of the disease averaged 10 years. The frequency of RA seropositivity for rheumatoid factor in the blood was 75%, and for antibodies to citrulline cyclic peptide was 73%. The І, ІІ and ІІІ degrees of disease activity, respectively, were stated in 26%, 44% and 30% of cases, and the ratio of I, II, III and IV stages of the pathological process was 1: 6: 5: 3. Results. We studied the physicochemical adsorption-rheological properties of the oral fluid (surface tension, viscoelasticity, relaxation), the levels of pro-inflammatory cytokines in it, the concentration of aminopeptide, peptide, nucleotide and chromatophore fractions of average weight molecules, the ratio with similar parameters in blood serum. To assess the number of aerobic and facultative anaerobic microorganisms vegetating on the gingival mucosa, the method of sterile paper discs was used. Compared with healthy people in the control group, 99% of the number of RA patients has an increase in the concentration of interleukin 1b in the oral fluid (22 times), 98% of tumoronecrotic factor a (7 times), 43% of the average increase in the average weight molecules (by 47%), at the same time, the parameters are associated with the presence of secondary Sjogren's syndrome, with the severity of the course of both articular syndrome, and periodontitis, which is accompanied by a longer relaxation time of oral washings (by 36%), but their lower interfacial activity (by 11%), the ratio of the tumor necrosis factor parameter a with the level of this pro-inflammatory cytokine in the blood (by 70%), and there are dispersion relationships of individual indicators of the oral fluid with the vegetation on the gums of bacterioids, bifidobacteria, Escherichia coli, megaspheres, moraxellas, neisseria, propionibacteria, proteus, pseudomones, sarcins and streptococcus. Conclusions: the obtained data dictate the need to develop new approaches to medical technology for individual pathogenetic therapy of chronic generalized periodontitis, taking into account its etiology in RA patients, identifying criteria that allow assessing the activity of the pathological process, predicting the nature of the course of the disease and the effectiveness of treatment measures.

scholarly journals Clinical and pathogenetic features of the joint syndrome in rheumatoid arthritis with comorbid periodontitis

TRAUMA ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 10-15
Author(s):  
O.V. Syniachenko ◽  
M.V. Yermolaieva ◽  
D.O. Havilei ◽  
K.V. Liventsova ◽  
S.M. Verzilov ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Oral Cavity ◽  
Rheumatoid Factor ◽  
Cyclic Peptide ◽  
Disease Onset ◽  
Pathological Process ◽  
Cytokine Network ◽  
X Ray ◽  
Anaerobic Microorganisms ◽  
Gingival Mucosa

Background. The accumulation of bacteria in the oral cavity of patients with rheumatoid arthritis (RA) increases the incidence of periodontal tissue lesions, but there is also an inverse relationship between both diseases when comorbid periodontitis contributes to the occurrence of RA. Endotoxemia caused by periodontitis can increase the sensitivity of RA patients to autoantigens and participate in the pathogenetic structures of both diseases. RA and periodontitis have similar osteoclasia, allelic genes, and a general imbalance in the state of the cytokine network. The purpose and objectives of the study: to study the frequency and nature of the course of comorbid chronic generalized periodontitis in patients with RA, to determine its etiological factors, to assess the degree of its influence on clinical and X-ray sonographic signs of the articular syndrome. Material and methods. The study included 173 RA patients aged 18–76 years (average 46 years), among whom there were 20.8 % men and 79.2 % women. The duration from the disease onset averaged 10 years. The frequency of seropositivity of RA for rheumatoid factor in the blood was 74.6 %, and for antibodies to citrulline cyclic peptide — 72.8 %. The I, II, and III degrees of disease activity, respectively, were determined in 26.0, 43.9, and 30.1 % of cases, and the ratio of I, II, III, and IV stages of the pathological process was 1 : 6 : 5 : 3. To assess the number of aerobic and facultative anaerobic microorganisms vegetating on the gingival mucosa, the method of sterile paper discs was used. Clinical, X-ray and ultrasound examination of the joints, a study of biochemical, enzyme-linked immunosorbent and physicochemical parameters of blood and oral washings, determination of the integral severities of the underlying and comorbid pathology were carried out. Results. Chronic generalized periodontitis was diagnosed in 45.7 % of RA patients (the ratio of men to women is 1 : 4), the development of which was closely related to the activity and stage of articular syndrome, the presence of systemic osteoporosis, seropositivity of the disease for rheumatoid factor and antibodies to cyclic citrulline peptide, and in the etiology of periodontitis, Moraxella, Pseudomonas, Staphylococci, Streptococci, Bacterioids, Eubacteria, Fusobacteria, Propionibacteria, and Sarcina are of paramount importance, and in its pathogenesis — interleukin 1β. At the same time, the pathology of the oral cavity itself is involved in the pathogenetic constructions of osteoporosis, lesions of the maxillary, wrist, and knee joints, the formation of arthrocalcifications, and the development of Sjogren’s syndrome. Conclusions. The obtained data dictate the need to develop new approaches to medical technology of individual pathogenetic therapy of RA, taking into account the presence and etiology of comorbid periodontitis, identifying criteria that allow assessing the activity of the pathological process, predicting the nature of the course of the disease and the effectiveness of therapeutic measures.

scholarly journals Clinical and pathogenetic significance of the gingival microbiota in rheumatoid arthritis

2021 ◽  
Vol 9 (1) ◽  
pp. 37-43
Author(s):  
O.V. Syniachenko ◽  
M.V. Іermolaieva ◽  
D.O. Gaviley ◽  
K.V. Liventsova ◽  
S.M. Verzilov
Keyword(s):  
Rheumatoid Arthritis ◽  
Oral Cavity ◽  
Clinical Laboratory ◽  
Anaerobic Bacteria ◽  
Pathological Process ◽  
Quantitative Composition ◽  
Oral Microbiota ◽  
Mineral Density ◽  
Anaerobic Microorganisms ◽  
Gingival Mucosa

Background. The trigger factors of the development of rheumatoid arthritis (RA) may be infections associated with the carriage in the oral cavity of Actinomycetes, Campylobacter, Mycoplasma, Porphyromonas, Transducers, Proteus, Selemons, Streptococci, and Fusobacteria, but their role in the disease pathogenesis requires clarification. In turn, the quantity of cases of comorbid infectious pathology significantly increases in RA. The purpose was to study the qualitative and quantitative composition of the oral microbiota in RA, to assess the role of individual bacteria in the pathogenetic structures of the disease. Materials and methods. There were examined 121 RA patients aged 18–76 years (on ave­rage 49 years old), among whom there were 19 % men and 81 % women. The duration from the first manifestation of the disease averaged 10 years. The frequency of seropositivity of RA for rheumatoid factor in the blood was 81 %, and for antibodies to cyclic citrullinated peptide — 75 %. I, II, and III degrees of disease activity were determined in 13, 45, and 42 % of cases, respectively, and the ratio of I, II, III, and IV stages of the pathological process was 1 : 5 : 5 : 3. To assess the number of aerobic and facultative anaerobic microorganisms growing on the gingival mucosa, the technique of sterile paper discs was used. The kits “Mikro-La-Test”, “Streptotest-16”, “Anaero-Test 23”, “Neferm Test 24” were used. Results. The vegetation of associations of aerobes on the gums was found in 96.7 % of patients, anaerobic bacteria — in 91.7 %, the number of which is closely related to clinical, laboratory, and X-ray signs of RA, while the integral landscape of the gingival microbiota is influenced by the severity of the comorbid chronic generalized periodontitis, which affects the presence of Moraxella, Sarcina, Streptococci, and Eubacteria in the oral cavity, moreover, the number of anaerobes reflects the degree of RA activity and bone mineral density, and in the pathogenetic constructions of individual signs of the disease (the nature of the articular syndrome and damage to the periarticular tissues), Aerococci, Candida, Clostridia, Megasphaera, Propionibacteria, Sarcina, Fusobacteria, and Eubacteria are involved. Conclusions. The obtained data dictate the need to develop new approaches to medical techno­logy for individual etiopathogenetic therapy of RA with comorbid periodontitis, to identify criteria that allow assessing the activity of the pathological process, predicting the nature of the course of the disease and the effectiveness of therapeutic measures.

scholarly journals AB0347 INCREASING TO OPTIMAL METHOTREXATE DOSE MIGHT BE A BETTER TRADITIONAL DMARD STRATEGY IN RA TREATMENTS: A RANDOMIZED CASE-CONTROL TRIAL OF HAKKA PEOPLE IN SOUTHERN CHINA

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1473.1-1473
Author(s):  
D. Lin ◽  
Y. Wen ◽  
Y. Zhang ◽  
Q. Chen ◽  
Y. Pan ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Folic Acid ◽  
Side Effects ◽  
Oral Dose ◽  
Optimal Dose ◽  
Chinese Patients ◽  
Control Group ◽  
Average Weight ◽  
Methotrexate Dose ◽  
Experimental Group

Background:The optimal methotrexate (MTX) dose is defined as 0.3mg/kg/week or ≥ 20mg/week at 6 months. [1] Considering average weight of Chinese, [2] the optimal MTX should be >15mg/w. However, not more than 30% in 25191 RA cases ever had MTX treatment in CREDIT (Chinese Registry of Rheumatoid arthritis). [3] The biggest concern is side effects of MTX. Our study is to investigate whether increasing MTX would get better results accompanied with more side effects to Chinese people.Objectives:Hakka people have the purest genes of the majority people-Han in China. It is planned to recruit 160 RA patients in Meizhou, where is a gathering place of Hakka people.Methods:The RA volunteers had no relief with 10 mg/w oral dose of MTX with/without other 1-2 inadequate dose of DMARDs for at least 3 months. They were randomly divided into 1:1 groups*. The experimental group would be treated with original DMARDs and incremental MTX (gradually increased to the optimal oral dose (0.3 mg/k/w) in the first 12 weeks and folic acid (the dose adjusted on demand with range from 5 mg/w to 5mg tid). While the control group would be treated with original MTX dose(10mg/w) but incremental original DMARDs(gradually increased to the maximum dose in the first 12 weeks). The two groups would keep the treatment at 12thweek last to the 36thweek, and the efficacy and safety indexes would be evaluated during the whole study.Results:1)We planned to recruit 160 RA patients in our study. 46 Hakka RA patients were enrolled in the study so far. 2 of 46 finished the 24thweek visit and 24 finished the 36thweek visit. The average age is 54.2± 9.3 years old, the average weight is 59.1±11.1kg, and the female to male ratio is 41:5.2)The average Folic acid dose is 14.4±9.5mg/w in the experimental group at the 12thweek.3)The morning stiffness time, PGA, PhGA, HAQ, DAS28 were better in experimental group after 12 weeks though slightly worse during 0-12 weeks. 100%(12) patients in experimental group, while 66.67%(8/12) in control group reach ACR20.4) Only 1 case(5.9%,1/23)had adverse event while 6 cases (26%,6/23) occurred adverse events. All events were mild level. 1 case (4.2%,1/23) in control group withdrew from the study because the disease was getting worse during 0-24 weeks.Conclusion:Hakka patients in China might have better outcomes due to increasing MTX to the 0.3mg/kg/w dose than increasing the other DMARDs. Therefore, We recommended the Chinses patients choose MTX as first incremental DMARD. The appropriate dose of Folic acid plus with the optimal dose of MTX in our study is higher than previous studies (such as 13.0±4.8mg/w reported by Gaujoux-Viala, 2018[1]). We recommended Chinese patients take 15mg/w folic acid to prevent MTX side effects in view of lower folic acid level in Chinese population.[3]References:[1]Gaujoux-Viala C, Rincheval N, Dougados M, et al. Optimal methotrexate dose is associated with better clinical outcomes than non-optimal dose in daily practice: results from the ESPOIR early arthritis cohort. Ann Rheum Dis. 2017 Dec;76(12):2054-2060.[2]Nan Jiang, Mengtao Li, Yanhong Wang, et al. Baseline characteristics and treatments among patients with rheumatoid arthritis: the CREDIT study in China, 2016-2018. Ann Rheum Dis. 2019 Jun; 78 (Suppl 2) 1404-1405.[3]He Y, Pan A, Hu FB,et al. Folic acid supplementation, birth defects, and adverse pregnancy outcomes in Chinese women: a population-based mega-cohort study, Lancet, 2016 Oct,Volume 388, Number 1, pp. S91-S91-(1)Disclosure of Interests:None declared

MINERAL HOMEOSTASIS OF THE ORAL FLUID IN CHILDREN AND ADOLESCENTS WHO HAVE UNDERGONE TREATMENT OF MALIGNANT NEOPLASMS

2021 ◽  
pp. 99-106
Author(s):  
Obukhov Yu.A. ◽  
Zhukovskaya E.V. ◽  
Karelin A.F.
Keyword(s):  
Children And Adolescents ◽  
Oral Fluid ◽  
Chemical Elements ◽  
Morphological Changes ◽  
Pathological Process ◽  
Main Group ◽  
Control Group ◽  
Malignant Neoplasms ◽  
Mineral Homeostasis ◽  
Toxic Damage

Annotation. The authors' study highlights the urgent problem of the development of toxic damage to the dentition under the influence of anticancer therapy in children and adolescents. The aim of the study was to study the violation of the homeostasis of the oral fluid and the severity of the carious process. Materials and methods. As part of a pilot study at the Russian Field Treatment and Rehabilitation Center, 63 children were studied the severity of mineral homeostasis disorders and damage to the dentoalveolar system. Results. In 67% of patients in the main group, there was an increased tendency to form carious cavities. 54.5% of them have complicated caries. One third of patients had more than five carious teeth. In the control group, carious lesions of the teeth in only three patients (20%), with the involvement of 1-3 teeth in the pathological process. Violation of enamel formation was diagnosed in 36% of children of the main group. There is a tendency for the accumulation of the studied microelements in the oral fluid, while in the blood serum there is a normal or insignificant decrease in their content. According to the literature, this may indicate destruction in the oral cavity. Deviations in the homeostasis of chemical elements in children who completed the treatment of malignant neoplasms and children in the control group were revealed. An increased content of osteotrophic microelements was found to correlate with the destructive processes of the teeth. Conclusion. It seems appropriate to study the severity of morphological changes in hard tissues of teeth and the electrolyte composition of mixed saliva in children and adolescents who have been cured of malignant neoplasms. The results of studies of gastric cancer, as an indicator of metabolic disorders, will make it possible not only to reveal the imbalance of macro- and microelements, but also to establish the effectiveness of adaptation mechanisms aimed at normalizing the elemental composition of the oral fluid.

scholarly journals RESULTS DYNAMICS RESEARCH ACTIVITY OF ANTIOXIDANT ENZYMES AND LEVELS OF ORAL FLUID MALONOVOHO DYALDEHIDU IN PATIENTS WITH GENERALIZED PERIODONTITIS AND RHEUMATOID ARTHRITIS WHO LIVE IN ECOLOGICALLY UNFAVORABLE AREA UNDER THE INFLUENCE OF COMPLEX TREATMEN

2020 ◽  
pp. 85-91
Author(s):  
K. V. Kovalyshyn ◽  
M. M. Rozhko
Keyword(s):  
Rheumatoid Arthritis ◽  
Antioxidant System ◽  
Oral Fluid ◽  
Malonic Dialdehyde ◽  
The State ◽  
Control Group ◽  
Comprehensive Treatment ◽  
Antioxidant Protection ◽  
Healthy Individuals ◽  
Group I

The aim of our research was to study the indicators changes in antioxidant protection and malonic dialdehyde (MDA) levels in the oral fluid in patients with  GP and rheumatoid arthritis (RA) who live in environmentally unfavorable areas and  assessment of the dynamics of changes in the studied indicators in the oral fluid under the influence of comprehensive treatment. Materials and methods. 105 patients with GP were examined and treated, of which 70 patients were with RA. Depending on the ecological living conditions of patients with RA was divided into two groups: group I (n = 35) - live in environmentally unfavorable areas; group II (n = 35) - live in environmentally favorable areas. Group III (n = 35) was formed by patients with GP without concomitant pathology. Each of the groups, depending on the severity of GP was divided into subgroups: A - GP of the I degree; B - GP of the II degree of severity. The control group consisted of 18 virtually healthy individuals with intact periodontium and preserved dentition. The state of the prooxidant system of the oral cavity was determined by the level of MDA. To study the state of the antioxidant defense system (AOS), the activity of superoxide dismutase (SOD) and catalase was determined. Research results.  Determining the level of MDA in oral fluid in all subgroups of patients with GP and RA and without concomitant pathology revealed an increase in this indicator before treatment. It increased most significantly in the IB subgroup to 1.19 ± 0.02 nmol/l and in the IA subgroup to 1.16 ±0.01 nmol/l, compared with 0.34 ± 0.17 nmol / l in healthy individuals. (* pIB-3 <0.001, * pIA-3 <0.001), which exceeded it by 3.5 and 3.4 times, respectively, which may be due to the negative impact of the environment. During all follow-up periods after conducted treatment, the level of MDA decreased and most closely approached the rate of healthy individuals after 6 months. However, before treatment, depletion of the antioxidant system was detected in all subgroups, as evidenced by the weakening of the AOS. In particular, in the IB subgroup there is the greatest decrease in the activity of SOD to 35.6 ± 0.56%, compared with the group of healthy individuals 49.06 ± 0.03% (p <0.001) and catalase to 4.42 ± 0.03 с.u along with healthy - 6.64 ± 0.01 c.u (p <0.001). The activity of certain antioxidant protection enzymes tends to increase after a comprehensive treatment in all subgroups after 6 months. And in the IB subgroup the activity of SOD after 6 months was 44.77 ± 0.35%, catalase 6.29 ± 0.03c.u. Conclusions. The results of the conducted biochemical researches of oral fluid in patients with GP and  RA, who live in environmentally unfavorable areas, showed a decrease in the activity of SOD enzymes, catalase and increased levels of MDA in these patients. After the conducted complex treatment there is a normalization of the AOS system, which is manifested in increased activity in the oral fluid SOD and catalase, as well as a decrease in MDA, which indicates a decrease in inflammation process and the effectiveness of our treatment and prevention complex on the antioxidant system, which provided remission GP in these patients.

scholarly journals Monitoring of changes in mineral and vitamin metabolism under the influence of feed additive in piglets with experimental associated mycotoxicosis

2020 ◽  
pp. 131-139
Author(s):  
A. Andriichuk ◽  
A. Melnyk ◽  
N. Vovkotrub
Keyword(s):  
Growth Rate ◽  
Fumonisin B1 ◽  
Pathological Process ◽  
Feed Additive ◽  
Control Group ◽  
Average Weight ◽  
Weight Growth ◽  
Calcium Phosphorus ◽  
Fusarium Fungus ◽  
Biological Accessibility

Toxicobiological effect of mycotoxins association of the Penicillium and Fusarium fungus (T-2 toxin at a concentration of 0.1 mg/kg, fumonisin B1 ‒ 0.5 mg/kg, vomitoxin (DON) ‒ 0.1 mg/kg, penicillic acid ‒ 1 mg/kg) was accompanied by the development of a complex pathological process in weaned piglets. In this regard, the detoxification and sorption capacity of the complex feed additive "Harufix+" based on mannanoligosaccharides was studied. The additive effect on the resorptive activity of mineral and vitamin nutrients of feed under the normal feeding conditions and in case of contamination with mycotoxins has been studied. The use of enterosorbent offset the toxic effects of micromycete metabolites, which contributed to the growth of piglets. Thus, weight growth rate increase of the piglets in group 1 (i.e., those whose diet included the additive, unlike the diet of the animals in control group) constituted 16 %, while their average weight growth rate was high and constituted 1.96 kg per day. In addition, during the study of calcium, phosphorus, magnesium, ferum, zinc, copper and manganese in the piglets blood was not found excretion of these elements with a sorbent, moreover, noted the normalization their blood level. The study content of vitamins A and E, the same as with mineral nutrients, has not been established decrease during treatment with study the pharmaceutical. The obtained results testify the active absorption in the gastrointestinal tract of the vitamin components within the fodder combined with fodder additive “Harufix+” and high biological accessibility of its transport forms. The efficiency of the additive can be explained by its composition, namely the complex of mineral and organic components that are formed by modification of the organic cations of the mineral surface. Key words: mycotoxins, mycotoxicosis, macro- and microelements, sorbent, vitamin metabolism, piglets.

scholarly journals MODERN DIAGNOSTICS METHODS OF NON-SPECIFIC PROTECTION OF PERIODONTAL TISSUES IN RESIDENTS OF THE INDUSTRIAL REGION

2020 ◽  
pp. 18-23
Author(s):  
A.V. Samoilenko ◽  
S.V. Pavlov ◽  
I.V. Vozna
Keyword(s):  
Oral Cavity ◽  
Oral Fluid ◽  
Pathological Process ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Study Group ◽  
Periodontal Tissue ◽  
Oral Liquid ◽  
Healthy Control

The aim of the article is to study the peculiarities of antimicrobial immunity of the oral cavity of the industrial region’s inhabitants in order to optimize special preventive programs of major dental diseases. The object and research methods. 178 patients was examined from 21 to 50 years old, who turned to the University Dental Center of Zaporizhzhia State Medical University. The study group consisted of 126 patients with generalized periodontitis of the initial (8), I (32), II (68) and III (18) degree of chronic course, complicated by harmful factors of manufacturing. The comparison group consisted of 32 patients with periodontitis (5), I (10), II (11) and III (6) degrees who did not work in adverse conditions. The control group consisted of 20 relatively healthy individuals aged from 19 to 25 years without signs of generalized periodontitis. To study the factors of local immunity of the oral cavity, oral fluid was collected from each examined person, which was obtained without stimulation, spitting into sterile tubes. Then the oral fluid was centrifuged for 15 minutes at 8,000 rpm. The supernatant part of the oral liquid was poured into plastic tubes and stored at 30°C. The quantitative determination of markers in the oral fluid was performed by standard enzyme immunoassay kits according to the instructions of the "Lactoferrin-strip" ("VectorBest") manufacturers. The result was expressed in mµg / ml. The content of cathelicidin LL-37 was determined by enzyme-linked immunosorbent assay using NycultBiotechhuman LL-37 ELISA (Netherlands) firm reagent kit-manufacturers. The result was expressed in µg / ml. Statistica 13.0 licensed number JPZ804I382130ARCN10-J was used to process the results. The results of the study and their discussion. The hygienic condition in the examined patients of the study group was assessed as unsatisfactory: from 2.24 ± 0.5 to 2.99 ± 0.47 points depending on the age and length of service at the enterprise, but the PI and SBI values were increased. It was found, that the lactoferrin level in the oral fluid was higher in the all patients with periodontitis, than the same index in comparison with healthy control group and consistently increased with increasing severity of the process. In the patients’ study group, working in hazardous conditions of production, the lactoferrin content has increased in the oral fluid relative to the level of the healthy patients was more pronounced in comparison with the patients, suffering of periodontal tissue disease, but do not work in harmful manufacturing conditions. Increased lactoferrin in the oral fluid in the steelmaking workers can be considered as a means of compensation, that provides protection of the oral mucosa from colonization of microorganisms. In the steel industry workers, a statistically significant cathelicidin content decrease in the oral fluid was observed compared to healthy control patients’ group. In the clinical group of the patients without adverse factors of production, the cathelicidin’s concentration in the oral fluid was also reduced. The increasing level of lactoferrin in the oral fluid in parallel with the cathelicidin’s decrease in saliva are markers of the inflammatory phase, as well as the destructive phase of connective tissue. Conclusions. Thus, our observations have established a direct relationship between the lactoferrin’s concentration in the oral fluid and the severity of periodontal tissue diseases and the feedback between the cathelicidin’s content in the oral liquid and the activity of the pathological process. The determination of lactoferrin and cathelicidin in the oral fluid of the patients with harmful manufacturing factors allow us to identify the signs of pathological process in the oral cavity.

Circulating Insulin-like Growth Factor-1 Levels in Patients with Rheumatoid Arthritis: A Meta-analysis

2019 ◽  
Vol 25 (10) ◽  
pp. 1091-1098 ◽  
Author(s):  
Yu-Lan Zhao ◽  
Jun Wu ◽  
Tian-Ping Zhang ◽  
Qian-Yao Cheng ◽  
Xue-Ping Wang ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Growth Factor ◽  
Fixed Effects ◽  
Meta Analysis ◽  
Pathological Process ◽  
Cochrane Library ◽  
Control Group ◽  
Insulin Like Growth Factor ◽  
Standard Mean Difference ◽  
Significant Difference

Conclusion: Patients with RA have lower circulating IGF-1 level than healthy controls, particularly for patients from Asia and Europe. Further studies are necessary to elucidate the role of IGF-1 in the pathological process of RA. Results: A total of eleven articles with 334 cases and 261 controls were finally included. Compared with the healthy group, the RA group had lower circulating IGF-1 levels (pooled SMD= -0.936, 95% CI= -1.382 to -0.489, p<0.001). The subgroup analysis showed that RA patients from Asia (SMD= -0.645, 95% CI= -1.063 to -0.228, p= 0.002) and Europe (SMD= -1.131, 95% CI= -1.767 to -0.495, p<0.001) had lower circulating IGF-1 levels, no significant difference in plasma/serum IGF-1 levels was observed in RA patients from America. Sensitivity analysis indicated the stability and credibility of the overall effect sizes. Methods: PubMed, Embase and the Cochrane Library databases were searched up to December 2018 in English, and the studies comparing serum/plasma IGF-1 levels between RA group and healthy control group were what we are interested in. The Newcastle-Ottawa Scale (NOS) was used to assess the methodological quality of the included studies. The heterogeneity test was performed by the Cochrane Q statistic and I2 –statistic. The publication bias was evaluated by the funnel plot and Egger’s test. The standard mean difference (SMD) with 95% confidence interval (CI) was calculated by the fixed-effects or random-effects model. Background and Objectives: Insulin-like growth factor-1 (IGF-1) levels have been investigated in rheumatoid arthritis (RA), however, produced inconsistent results. The purpose of this meta-analysis was to derive a more precise conclusion about serum/plasma IGF-1 levels in RA patients.

Effect of Dietary Education on Cardiovascular risk Factors in Rheumatoid Arthritis Patients

2020 ◽  
Vol 16 ◽  
Author(s):  
Rahil Taheri ◽  
Shahram Molavynejad ◽  
Parvin Abedi ◽  
Elham Rajaei ◽  
Mohammad Hosein Haghighizadeh
Keyword(s):  
Rheumatoid Arthritis ◽  
Risk Factors ◽  
Blood Pressure ◽  
Cardiovascular Risk ◽  
Cardiovascular Risk Factors ◽  
Statistical Tests ◽  
Intervention Group ◽  
Serum Levels ◽  
Control Group ◽  
Dietary Education

Aim: The aim of this study was to investigate the effect of dietary education on cardiovascular risk factors in patients with rheumatoid arthritis. Method: In this randomized clinical trial, 112 patients with rheumatoid arthritis were randomly assigned into two groups, intervention and control. Dietary education was provided for the intervention group in 4 sessions; anthropometric measurements, serum levels of RF, triglycerides, cholesterol, HDL, LDL, and fasting blood sugar were measured before and three months after intervention. Data was analyzed using SPSS software and appropriate statistical tests. Results: The mean of total cholesterol (p <0.001), triglycerides (p = 0.004), LDL (p <0.001), systolic blood pressure (p = 0.001), diastolic blood pressure (p = 0.003), FBS and BMI (p <0.001) were decreased significantly in the intervention group after education compared the control group. Conclusion: Traditional care for rheumatoid arthritis patients is not enough. Patients need more education in order to improve their situation.

Can Cytokines be used as an Activation Marker in Rheumatoid Arthritis?

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatih Öner Kaya ◽  
Yeşim Ceylaner ◽  
Belkız Öngen İpek ◽  
Zeynep Güneş Özünal ◽  
Gülbüz Sezgin ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Healthy Volunteers ◽  
Venous Blood ◽  
Cross Sectional Study ◽  
Joint Disease ◽  
Control Group ◽  
Cross Sectional ◽  
Positive Correlation ◽  
Das 28 ◽  
Tnf Α

Aims: The etiopathogenesis of Rheumatoid Arthritis (RA) is not clearly understood. However, the role of the cytokines takes an important part in this mechanism. We aimed to bring a new approach to the concept of 'remission' in patients with RA. Background: RA is a chronic, autoimmune, inflammatory disease that involves small joints in the form of symmetrical polyarthritis and progresses with exacerbations and remissions. Pain, swelling, tenderness and morning stiffness are typical of the joints involved. Although it is approached as a primary joint disease, a wide variety of extra-articular involvements may also occur. It is an interesting pathophysiological process, the exact cause of which is still unknown, with many environmental, genetic and potentially undiscovered possible factors in a chaotic manner. Objective: In this cross-sectional study, sedimentation rate (ESR), C- Reactive protein (CRP), Tumor necrosis factor (TNF)-α, soluble-TNF-α receptor (TNF-R), Interleukin (IL)-1B and IL-10 were measured in three groups which were healthy volunteers, patients with RA in the active period, and patients with RA in remission. Disease activity score-28 (DAS-28) was calculated in active RA and RA in remission. Methods: This study included 20 healthy volunteers, 20 remission patients with RA and 20 active RA patients. Venous blood samples were collected from patients in both healthy and RA groups. Results: RA group consisted 43 (71.6%) female and 17 (28.4%) male. Control group consisted 11 (55%) female and 9 (45%) male. TNF-R was significantly high only in the active group according to the healthy group (p=0.002). IL-10 was significantly high in active RA according to RA in remission (p=0.03). DAS-28 was significantly high in active RA according to RA in remission (p=0.001). In the active RA group, ESR and TNF-R had a positive correlation (r:0.442; p=0.048). In the active RA group, there was also a positive correlation between TNF-R and CRP (r:0.621; p=0,003). Both healthy and active RA group had significant positive correlation between ESR and CRP (r: 0.481; p=0.032 and r: 0,697; p=0,001 respectively). Conclusion: TNF-R can be the main pathophysiological factor and a marker showing activation. TNF-R can be very important in revealing the effect of TNF on the disease and the value of this effect in the treatment and ensuring the follow-up of the disease with CRP instead of ESR in activation.

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