scholarly journals Haematological and biochemical profile of Friesian x Bunaji heifers supplemented with inorganic selenium and vitamin E

2020 ◽  
Vol 46 (2) ◽  
pp. 80-86
Author(s):  
N. P. Achi ◽  
P. P. Barje ◽  
M. Abdulrashid ◽  
O. M. Daudu
Keyword(s):  
Vitamin E ◽  
Blood Cells ◽  
Biochemical Analysis ◽  
White Blood Cells ◽  
Biochemical Profile ◽  
Randomized Design ◽  
Inorganic Selenium ◽  
Healthy Cattle ◽  
Completely Randomized Design ◽  
Apparently Healthy

A study was conducted using twenty Friesian x Bunaji heifers to evaluate the effect of supplementing inorganic feed grade Selenium and Vitamin E on the haematological and biochemical profile. The study consists of four treatments with five animals per Treatment in a Completely Randomized Design (CRD). Treatment 1 served as Control without Selenium or Vitamin E supplementation. Treatment 2 was supplemented with 3 mg of feed gradeSelenium. Treatment 3 with 20 mg of feed grade Vitamin E. Treatment 4 had a combination of 3 mg feed grade Selenium and 20 mg of Vitamin E. Two sets of blood samples were taken from the animal via jugular venipuncture using a 10 mL syringe fitted with a needle into sample bottles for haematological and biochemical analysis. Neutrophils (19.78 - 29.88%) were similar (p<0.05) and Se supplementation recording the highest value. Packed cell volume(19.14 – 22.22%), Haemoglobin, Red blood cells, White blood cells, Eosinophil, Monocytes, Basophil and Lymphocytes analyzed were similar (p>0.05). It was observed that there were similarities (p>0.05) in Glucose, Serum protein, globulin, urea N, albumin, MDA protein, AST, ALT and ALP analyzed except for GSH-Px (22.38 – 24.70 μ/ml). Glutathione peroxidase was higher (p<0.05) in Friesian x Bunaji heifers supplemented with vitamin E and lowest with no supplementation. All parameters analyzed were within the normal range for apparently healthy cattle thereby suggesting that Selenium and Vitamin E as antioxidant are effective in scavenging for free radicals and reducing oxidative damage. 

scholarly journals The Effect of Breadfruit Leaf Flour (Artocarpus altilis) on Number of Blood Cells And Correlation Between Cholesterol Blood and Meat of Tegal Ducks 10 Weeks Age

2018 ◽  
Vol 19 (3) ◽  
pp. 179
Author(s):  
Elly Tugiyanti ◽  
Emmy Susanti
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regression Coefficient ◽  
White Blood Cells ◽  
Blood Cholesterol ◽  
Blood Profile ◽  
Artocarpus Altilis ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design

The purpose of this research was to know the effect of breadfruit leaves flour in feed to blood profile and correlation between cholesterol, HDL and LDL of blood with meat cholesterol of maleTegal duck of 10 weeks old. The material used was Day Old Duck of male Tegal duck  as much as 120 heads with experimental research methods and Completely Randomized Design (CRD). There were 6 treatments: feed without breadfruit leaf powder, feed + breadfruit flour 3% / kg feed, feed + breadfruit flour 6% / kg feed, feed + breadfruit flour 9% / kg feed, feed + flour breadfruit 12% / kg feed, and feed + breadfruit flour 15% / kg feed. Each treatment was repeated 5 times and each replication consisted of 4 ducks. The variables observed were the number of red blood cells, white blood cells, HDL, LDL, blood triglycerides, correlation between cholesterol, HDL and LDL blood with meat cholesterol of male Tegal duck of 10 weeks old.  The result of the analysis showed that the supplementation of breadfruit leaves has significant effect (P <0,05) on the number of red blood cells, white blood cells, HDL, and blood triglyceride. There is a positive correlation between blood cholesterol with duck meat cholesterol which given breadfruit leaf powder. This research can be concluded that the supplementation of breadfruit leaf powder (Artocarpus altilis) can increase the number of red blood cells, white blood cells, blood HDL level but lowers blood cholesterol and triglyceride blood of male Tegal duck of 10 weeks old. Cholesterol meat is affected by blood cholesterol with regression coefficient of 0.780941, influenced by LDL (r = 0.407849), but negatively affected by HDL (r = -0.47022).

scholarly journals Pengaruh Konsentrasi IgY Induk yang Berbeda Terhadap Gambaran Darah Anak Ayam Sentul

2019 ◽  
Vol 6 (2) ◽  
pp. 273
Author(s):  
Melly Pratiwi Setyawati ◽  
Niken Ulupi ◽  
Sri Murtini ◽  
Cece Sumantri
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
T Test ◽  
Blood Picture ◽  
Test Program ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference

ABSTRAKPenelitian ini bertujuan untuk menganalisis gambaran sel darah putih dan sel darah merah sebagai indikator ketahanan tubuh pada anak ayam sentul. Penelitian ini menggunakan 20 ekor ayam betina dan 20 ekor ayam jantan yang dikawinkan, masing masing terdiri dari 10 ekor ayam dengan konsentrasi IgY tinggi dan 10 ekor ayam dengan konsentrasi IgY rendah. Berdasarkan hasil perkawinan tersebut diperolah 34 ekor anak ayam yang terdiri dari 17 ekor anak ayam yang berasaldari induk dengan konsentrasi IgY rendah. Parameter yang diamati meliputi gambaran sel darah merah (eritrosit, hematocrit dan indeks eritrosit) dan gambaran sel darah putih (leukosit dan diferensiasinya). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 2 perlakuan, semua perlakuan diulang 10 kali. Data yang diperoleh dianalisis menggunakan t-test program SAS.  Hasil penelitian ini menunjukan bahwa perbedaan konsentrasi IgY pada induk tidak mempengaruhi sel darah merah dan sel darah putih. Namun terdapat kecendrungan nilai limfosit yang rendah dan H/L yang tinggi pada anak ayam yang berasal dari induk dengan konsentrasi IgY rendah. Hasil ini mengindikasi bahwa anak ayam yang berasal dari induk dengan konsentrasi IgY rendah lebih rentan terhadap cekaman panas. Kata Kunci: ayam sentul, IgY,  gambaran darah, ketahanan tubuhABSTRACTThis study was to aim the description of white blood cells and red blood cells as indicators of immunity in Sentul chicks. This study used 20 hen chickens and 20 cock-chicken mated, consisting of 10 chickens with high IgY concentration and 10 chickens with low IgY concentrations. Based on the results of mating, 34 chicks were obtained consisting of 17 chicks that were from the hen with low IgY concentration. The parameters observed included the description of red blood cells (erythrocytes, hematocrit and erythrocyte index) and the description of white blood cells (leukocytes and their differentiation). The experiment was conducted as a completely randomized design with 2 treatments, all treatments were repeated 10 times. The data obtained were analyzed using the SAS program t-test. The results of this study indicate that the difference in IgY concentration in the hen did not effect on red blood cells and white blood cells. However, there is a tendency for low lymphocyte values and high H/ L in chicks originating from hens with low IgY concentrations. These results indicate that chicks from hens with low IgY concentrations are more susceptible to heat stress.Keyword: blood picture, IgY, immunity, sentul chicken

scholarly journals Haematological and biochemical indices of broiler chicken fed graded levels of boiled African yam beans (Sphenostylis stenocarpa)

2020 ◽  
Vol 44 (2) ◽  
pp. 139-144
Author(s):  
A. S. Eburuaja ◽  
D. N. Onunkwo ◽  
O. O. Adedokun
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Cells ◽  
Broiler Chicken ◽  
White Blood Cells ◽  
Treatment Groups ◽  
Randomized Design ◽  
Optimal Productivity ◽  
Mean Corpuscular Haemoglobin ◽  
Completely Randomized Design ◽  
Biochemical Indices

This study was carried out to investigate the haematological and biochemical indices of broiler chicken fed graded levels of boiled African yam beans. The birds, 120 in number were allotted into four treatment groups having three replicate per treatment and 10 birds per replicate in a completely randomized design were used. Diet 1 was the control and corn-soy bean-based while diets 2, 3, and 4 had boiled African am beans at 10, 15 and 20% respectively. Blood samples were collected from eight weeks old broiler chicken into two sets of labeled sterile bottles and used for the analysis. The values observed for white blood cells (2.43, 2.71, 2.72. 2.76), packed cell volume (31.00, 31.04, 28.00, 27.00) and mean corpuscular haemoglobin (32.83, 32.70, 29.60, 33.93%) for diets 1, 2, 3 and 4 respectively fell within the normal range reported for healthy broiler chicken while the red blood cells for diets 1, 2, and 3 are comparable. Blood of birds in Diets 2, 3 and 4 had similar values of alkaline phosphatase which were higher than those of diet 1. Those in diets3 and 4 had highest values of urea (30.00mg/dl), creatinine (0.413mg/dl) and lowest values of albumen (1.500d/L). Feeding boiled African yam bean above 10% level of inclusion resulted in increase in alkaline phosphatase, urea and creatinine. For optimal productivity 10% inclusion of boiled African yam beans is therefore recommended.

Haematological and serum biochemical indices of growing rabbits fed graded levels of Yam peel meal as replacement for maize

2020 ◽  
Vol 47 (4) ◽  
pp. 167-175
Author(s):  
I. B. Amaza ◽  
A. Maidala ◽  
C. E. Isidahomen
Keyword(s):  
Biochemical Analysis ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Feeding Trial ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Inclusion Level ◽  
Completely Randomized Design ◽  
Biochemical Indices ◽  
Dietary Treatments

Haematological and serum biochemical parameters are indicators of physiological, nutritional and pathological status of animals. Investigation of these parameters provide information for diagnostic purposes. The study was conducted to determine the influence of feeding graded levels of yam peel meal (YPM) as a replacement for maize on haematology; blood chemistry was evaluated in rabbits. Thirty- six (New Zealand white X Chinchilla) were randomly assigned to four dietary treatments in a completely randomized design with nine rabbits per treatment. The rabbits were fed diets containing 0, 12.5, 25 and 37.5% of Yam peel meal (YPM) as a replacement for maize, designated as T1, T2, T3 and T4, respectively. The experimental diets and clean drinking water were supplied ad libitum throughout the experimental period of nine weeks. At the end of the feeding trial, three rabbits per treatment were randomly selected for hematological and serum biochemical analysis. All the haematological and serum biochemical indices except AST were affected (P<0.05) by dietary inclusion of YPM. Although most of the parameters investigated were affected by rabbits fed test ingredient, the values fell within the normal literature reference values for rabbits. It can therefore be concluded that up to 37.5% inclusion level of YPM can be fed to growing rabbits without deleterious effects on health status.

scholarly journals Biometric viscera and blood parameters of meat quails supplemented with inorganic selenium and vitamin E

2017 ◽  
Vol 18 (4) ◽  
pp. 560-575
Author(s):  
Vittor Zancanela ◽  
Antonio Claudio Furlan ◽  
Paulo César Pozza ◽  
Simara Márcia Marcato ◽  
Daiane de Oliveira Grieser ◽  
...  
Keyword(s):  
Vitamin E ◽  
Relative Weight ◽  
Blood Parameters ◽  
Linear Increase ◽  
Spleen Weight ◽  
Quadratic Effect ◽  
Randomized Design ◽  
Inorganic Selenium ◽  
Mineral Interaction ◽  
Different Levels

SUMMARY This study aimed to evaluate biometric viscera and blood parameters of quails at 14 and 35 days of age, supplemented with different levels of inorganic selenium and vitamin E. A completely randomized design was used in a 4×4 factorial scheme (inorganic Se = 0.1125, 0.2250, 0.3375 and 0.4500 mg/kg diet x VE = 10, 23, 36 and 49 IU/kg feed). In the 0-14 days experiment, 2,400 newborn quail were used, distributed in 16 treatments and three replications of 50 birds each. In the 14-35 days experiment, 1,680 14 day-old quails were used with same treatments and three replicates of 35 birds each. At 14 days, the relative spleen weights (P=0.0203) increased linearly as a function of the VE level, while at 35 days, the relative weight of the bursa (P=0.0390) increased linearly as a function of Se concentration. At 14 days, there was a vitamin x mineral interaction (p=0.0097) affecting total cholesterol (TC), and a quadratic effect (P = 0.0138) related to Se and linear reduction levels (P=0.0275) as a function of VE. At 35 days, the TC concentrations (P=0.0055) and triglycerides (TG, P = 0.0220) showed a quadratic effect for Se. The linear increase in spleen weight and bursa after 14 and 35 days respectively suggests an improved immune response as a function of VE and Se supplementation. To achieve the lowest concentration of TC and TG at 35 days of age, supplementation of 0.26 and 0.29 mg/Se/kg/feed, respectively, is recommended.

scholarly journals Efek Penambahan Vitamin E dalam Pengencer Glukosa Fosfat terhadap Daya Tahan Hidup Spermatozoa Domba pada Suhu 5 °C

2017 ◽  
Vol 9 (1) ◽  
pp. 25
Author(s):  
Nilawati Widjaya
Keyword(s):  
Plasma Membrane ◽  
Vitamin E ◽  
Analysis Of Variance ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Glucose Phosphate ◽  
Randomized Design ◽  
Completely Randomized Design

<p class="p1">The purpose of this study was to know the effect of vitamin E addition in glucose phosphate diluent on the survival of sheep spermatozoa at 5 °C. The xperiment used 3 rams. The design used was Completely Randomized Design (CRD) with 5 treatments and 4 replicates, each replicate with 3 ejaculate cement of sheep. The treatments were the dose of Vitamin E with E<span class="s1">0 </span>= 0 μg/ml, E<span class="s1">1 </span>= 1 μg/ml, E<span class="s1">2 </span>= 4 μg/ml, E<span class="s1">3 </span>= 7 μg/ml, and E<span class="s1">4 </span>= 10 μg/ml. Variables measured were motility, live spermatozoa and plasma membrane integrity. Data were analyzed by using analysis of variance according to Steel and Torrie (1981). The result showed that treatment of vitamin E with a dose of 1 μg/ml - 10 μg/ml l had no significant effect on motility, percentage live spermatozoa and membrane integrity of sheep spermatozoa on day two (P&gt; 0.05).</p>

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

Associations between white blood cells count and obesity in apparently healthy young adults

2020 ◽  
Vol 50 (6) ◽  
pp. 1013-1019
Author(s):  
Fatemeh Ghannadiasl
Keyword(s):  
Young Adults ◽  
Cardiovascular Diseases ◽  
Blood Cells ◽  
White Blood Cells ◽  
Cross Sectional Study ◽  
The Body ◽  
Sectional Study ◽  
Cross Sectional ◽  
Content Type ◽  
Apparently Healthy

Purpose The elevated white blood cells (WBCs) count has been reported to be a predictor of cardiovascular diseases, diabetes, hypertension and metabolic syndrome. This study aims to determine the associations between WBCs count and obesity in apparently healthy young adults. Design/methodology/approach In this cross-sectional study, the authors evaluated the body mass index (BMI) in 392 apparently healthy young adults of both sexes. The WBCs count was measured using standard counter techniques. The inclusion criteria were the agreement to participate in the study, between 18 and 25 years of age, lack of self-reported diseases such as cardiovascular diseases, hypertension, kidney and infectious diseases. Findings According to the BMI classification, underweight and overweight or obesity were observed in 14.58 and 11.48 per cent of young adults, respectively. The mean WBC was 6.5 ± 1.5 (×10³ cells/µL). Higher values of WBCs were found in women than in men (p = 0.02). The young adults with higher BMI had a higher WBCs count. There was a positive correlation between WBCs count and weight and BMI (r = 0.19 and r = 0.22, p < 0.001, respectively). Research limitations/implications This research was a cross-sectional study. Future studies are suggested using longitudinal studies to examine more relationships between obesity and WBCs count in apparently healthy young adults. Practical implications The results of this study provide evidence for weight management in this age group to reduce diseases associated with increased WBCs count. Originality/value The WBCs count was related to increasing levels of BMI per cent 2 C even in the normal range.

scholarly journals Growth and viability of juvenile humpback grouper (Cromileptes altivelis) supplemented with inorganic and organic selenium

2013 ◽  
Vol 11 (2) ◽  
pp. 141
Author(s):  
Muhaimin Hamzah ◽  
Muhammad Agus Suprayudi ◽  
Nur Bambang Priyo Utomo ◽  
Wasmen Manalu
Keyword(s):  
Sodium Selenite ◽  
Sea Water ◽  
Average Length ◽  
Stocking Density ◽  
Protein Retention ◽  
Organic Selenium ◽  
Randomized Design ◽  
Inorganic Selenium ◽  
Completely Randomized Design ◽  
Fish Performance

<p>This study was conducted to determine the effects of inorganic selenium (sodium selenite) and organic selenium (selenomethionine) supplementation on growth and viability of juvenile humpback grouper (Cromileptes altivelis). The experiment was designed as a completely randomized design with eight treatments and three replications. The treatment being tested was source and dosages of selenium ie., inorganic selenium (sodium selenite) supplementation with dosages of 0.5, 1, 2, and 4 mg Se/kg diet and organic selenium (selenomethionine) with dosages of 1, 2, and 4 mg Se/kg diet. Another treatment was unsupplemented selenium. Juveniles humpback grouper at an initial average length of 6,39±0,41 cm and body weight of 4,49±0,65 g were reared in 90×40×35 cm3 aquaria and fed artificial diet (pellet) two times daily (08.00 and 16.00) at satiation. Fishes were reared for 40 days at a stocking density of 12 fish per aquarium on sea water with salinity of 30–31 ppt and temperature of 28–29 °C. The results of this study showed that the source of selenium supplementation (inorganic or organic) affected growth performance, glutathione peroxidase (GPx) enzyme activity, and blood profiles of the experimental fish. Generally, it was found that selenomethionine supplementation resulted in better fish performance than sodium selenite. In sodium selenite supplementation, survival declined with the increased dosages of Se in the diet, and the supplementation at dosage of 0,5 mg Se/kg diet showed a toxic effects. Histopatological test showed that there were damage in livers, kidneys, and intestines of fish supplemented with sodium selenite from 0.5 to 4 mg Se/kg diet. On the contrary, supplementation of selenomethionine up to 4 mg Se/kg did not show any signs of toxicity and the survival was on 86.11 to 97.22%. Feed efficiency, protein retention, lipid retention, and Se retention indicated that a dosage of 4 mg Se/kg selenomethionine supplementation was the best dosage.</p><p>Keywords: selenium, viability, growth, Cromileptes altivelis, humpback grouper</p>

scholarly journals PENGARUH PENAMBAHAN LEVEL VITAMIN E-SELENIUM DALAM PAKAN TERHADAP FERTILITAS DAN DAYA TETAS TELUR HASIL PERSILANGAN AYAM SENTUL DENGAN AYAM RAS PETELUR

2019 ◽  
Vol 7 (3) ◽  
pp. 287
Author(s):  
Nining Haryuni ◽  
Anna Lidyawati ◽  
Binti Khopsoh
Keyword(s):  
Vitamin E ◽  
Experimental Method ◽  
Laying Hens ◽  
Selenium Level ◽  
Embryo Mortality ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hatching Eggs

The purpose of this research was to obtain a dose of vitamin E-selenium addition in feed on the quality of hatched eggs resulting from crossing Sentul chicken with laying hens. This research used 200 hatching eggs produced from the 34-week-old Isa Brown strain of laying hens which were treated and inseminated using Sentul chicken males. The experimental method used in this research was a completely randomized design CRD) with 5 treatments and 5 replications with each replication consisting of 8 hatching eggs. The treatments feed in this research were as follows: P0 = Basal Feed, P1 = Basal Feed + 21.75 ppm vitamin E and 0.23 ppm selenium, P2 = Basal Feed + 43.5 ppm vitamin E and 0.46 ppm selenium, P3 = Basal Feed + 65.25 ppm vitamin E and 0.69 ppm selenium, P4 = Basal Feed + 87 ppm vitamin E and 0.92 ppm selenium. The results of this research were that the addition of vitamin E-selenium levels in feed had no significant effect (P> 0.05) on the weight of hatching eggs, fertility, embryo mortality and hatchability.  Keywords: Vitamin E-Selenium Level, Feed, Hatching Eggs, Sentul Chicken ,Laying Hens

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