scholarly journals PENGARUH ASAP ANTI NYAMUK KERTAS BAKAR TERHADAP JUMLAHERITROSIT PADA MENCIT (Mus musculus L.)

2018 ◽  
Vol 2 (1) ◽  
pp. 40
Author(s):  
Choirun Niswah ◽  
Syarifah Syarifah ◽  
Fitria Sany
Keyword(s):  
Red Blood Cells ◽  
Mus Musculus ◽  
Blood Cells ◽  
Research Method ◽  
Insect Repellent ◽  
Mosquito Repellent ◽  
Tropical Country ◽  
Randomized Design ◽  
Teaching Faculty ◽  
Completely Randomized Design

Indonesia is a tropical country encountered species of mosquitoes, such as Aedes aegypti and Culex quinquefasciatus. One of the efforts made to eradicate mosquitoes is to use mosquito repellent. Mosquito repellent is one type of insect killers household pesticide (insecticide) containing dangerous transfultrin. This study aims to determine the effect of anti-mosquito smoke burn paper against the number of erythrocytes in mice (Mus musculus L.). The study was conducted in September 2016 in the Laboratory of Mathematics of Tarbiyah and Teaching Faculty of UIN Raden Fatah Palembang. The research method using a completely randomized design (CRD) with treatment P0 (without exposure to insect repellent), P1 (anti mosquito burn paper, 2 hours / day), P2 (anti mosquito burn paper, 4 hours / day), P3 (anti mosquito burn paper, 6 hours / day) each with 6 replications, for 20 days. On the last day to do the stabbing in the tail to draw blood, were calculated against the number of red blood cells (erythrocytes) by using Haemocytometer. The results showed that exposure to fuel anti-mosquito burn paper affect the red blood cells of mice that P1 (anti mosquito burn paper, 2 hours / day) with an average of 8,72 x 106 / mL blood, P2 (anti mosquito burn paper, 4 hours / day) with an average of 8,87 x 106 / mL blood, P3 (anti mosquito burn paper, 6 hours / day) with an average of 7,89 x 106 / mL of blood. Effect of anti-mosquito smoke burn the most significant papers in a decrease in the number of erythrocytes of mice (Mus musculus L.) is 6 hours of exposure to an average of 7,89 x 106 / mL. The result of Anova test showed F count > Ftable namely 7,80 > 4,40 it means the treatment has significance.

scholarly journals The Effect of Breadfruit Leaf Flour (Artocarpus altilis) on Number of Blood Cells And Correlation Between Cholesterol Blood and Meat of Tegal Ducks 10 Weeks Age

2018 ◽  
Vol 19 (3) ◽  
pp. 179
Author(s):  
Elly Tugiyanti ◽  
Emmy Susanti
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regression Coefficient ◽  
White Blood Cells ◽  
Blood Cholesterol ◽  
Blood Profile ◽  
Artocarpus Altilis ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design

The purpose of this research was to know the effect of breadfruit leaves flour in feed to blood profile and correlation between cholesterol, HDL and LDL of blood with meat cholesterol of maleTegal duck of 10 weeks old. The material used was Day Old Duck of male Tegal duck  as much as 120 heads with experimental research methods and Completely Randomized Design (CRD). There were 6 treatments: feed without breadfruit leaf powder, feed + breadfruit flour 3% / kg feed, feed + breadfruit flour 6% / kg feed, feed + breadfruit flour 9% / kg feed, feed + flour breadfruit 12% / kg feed, and feed + breadfruit flour 15% / kg feed. Each treatment was repeated 5 times and each replication consisted of 4 ducks. The variables observed were the number of red blood cells, white blood cells, HDL, LDL, blood triglycerides, correlation between cholesterol, HDL and LDL blood with meat cholesterol of male Tegal duck of 10 weeks old.  The result of the analysis showed that the supplementation of breadfruit leaves has significant effect (P <0,05) on the number of red blood cells, white blood cells, HDL, and blood triglyceride. There is a positive correlation between blood cholesterol with duck meat cholesterol which given breadfruit leaf powder. This research can be concluded that the supplementation of breadfruit leaf powder (Artocarpus altilis) can increase the number of red blood cells, white blood cells, blood HDL level but lowers blood cholesterol and triglyceride blood of male Tegal duck of 10 weeks old. Cholesterol meat is affected by blood cholesterol with regression coefficient of 0.780941, influenced by LDL (r = 0.407849), but negatively affected by HDL (r = -0.47022).

scholarly journals Pengaruh Konsentrasi IgY Induk yang Berbeda Terhadap Gambaran Darah Anak Ayam Sentul

2019 ◽  
Vol 6 (2) ◽  
pp. 273
Author(s):  
Melly Pratiwi Setyawati ◽  
Niken Ulupi ◽  
Sri Murtini ◽  
Cece Sumantri
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
T Test ◽  
Blood Picture ◽  
Test Program ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference

ABSTRAKPenelitian ini bertujuan untuk menganalisis gambaran sel darah putih dan sel darah merah sebagai indikator ketahanan tubuh pada anak ayam sentul. Penelitian ini menggunakan 20 ekor ayam betina dan 20 ekor ayam jantan yang dikawinkan, masing masing terdiri dari 10 ekor ayam dengan konsentrasi IgY tinggi dan 10 ekor ayam dengan konsentrasi IgY rendah. Berdasarkan hasil perkawinan tersebut diperolah 34 ekor anak ayam yang terdiri dari 17 ekor anak ayam yang berasaldari induk dengan konsentrasi IgY rendah. Parameter yang diamati meliputi gambaran sel darah merah (eritrosit, hematocrit dan indeks eritrosit) dan gambaran sel darah putih (leukosit dan diferensiasinya). Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 2 perlakuan, semua perlakuan diulang 10 kali. Data yang diperoleh dianalisis menggunakan t-test program SAS.  Hasil penelitian ini menunjukan bahwa perbedaan konsentrasi IgY pada induk tidak mempengaruhi sel darah merah dan sel darah putih. Namun terdapat kecendrungan nilai limfosit yang rendah dan H/L yang tinggi pada anak ayam yang berasal dari induk dengan konsentrasi IgY rendah. Hasil ini mengindikasi bahwa anak ayam yang berasal dari induk dengan konsentrasi IgY rendah lebih rentan terhadap cekaman panas. Kata Kunci: ayam sentul, IgY,  gambaran darah, ketahanan tubuhABSTRACTThis study was to aim the description of white blood cells and red blood cells as indicators of immunity in Sentul chicks. This study used 20 hen chickens and 20 cock-chicken mated, consisting of 10 chickens with high IgY concentration and 10 chickens with low IgY concentrations. Based on the results of mating, 34 chicks were obtained consisting of 17 chicks that were from the hen with low IgY concentration. The parameters observed included the description of red blood cells (erythrocytes, hematocrit and erythrocyte index) and the description of white blood cells (leukocytes and their differentiation). The experiment was conducted as a completely randomized design with 2 treatments, all treatments were repeated 10 times. The data obtained were analyzed using the SAS program t-test. The results of this study indicate that the difference in IgY concentration in the hen did not effect on red blood cells and white blood cells. However, there is a tendency for low lymphocyte values and high H/ L in chicks originating from hens with low IgY concentrations. These results indicate that chicks from hens with low IgY concentrations are more susceptible to heat stress.Keyword: blood picture, IgY, immunity, sentul chicken

scholarly journals Effectiveness of Sansavieria trifasciata Extract Bracelet in Absorbing Carbon Monoxide (Co) in Cigarette Smoke

Jurnal Biota ◽  
1970 ◽  
Vol 5 (2) ◽  
pp. 96-103
Author(s):  
Gendis Siti Rahmawati ◽  
Resti Nuraeni ◽  
Lu’lu Robiatul Fajrin ◽  
Sistiana Windyariani
Keyword(s):  
Carbon Monoxide ◽  
Red Blood Cells ◽  
Experimental Method ◽  
Blood Vessels ◽  
Cigarette Smoke ◽  
Blood Cells ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Randomized Design ◽  
Completely Randomized Design

Carbon monoxide (CO) contained in cigarette smoke is a very strong poison, if it is smoked by the lungs it will be easier to bind hemoglobin, so that red blood cells will lack oxygen, which can cause damage to blood vessels. with the process of atherosclerosis (narrowing). Sansevieria trifasciata has the ability to absorb poisons such as CO. This study aims to determine the effectiveness of Sansevieria trifasciata leaf extract in absorbing CO contained in cigarette smoke and assessing the amount of CO absorbed by Sansevieria trifasciata leaf extract. The method used is a pure experimental method using a Completely Randomized Design (CRD) consisting of 3 different treatments and 3 repetitions. The results of the 3 treatments showed that P1 (Sanseviera trifasciata wet extract) had the ability to absorb CO an average of 12.67 ppm every 15 minutes, in contrast to P2 (ethanol extract of Sanseviera trifasciata) which absorbs CO. 91.11 ppm every 15 minutes P3 (ethanol extract and CMC leaves from Sansevieria trifasciata) has the ability to absorb CO by 37.89 ppm every 15 minutes. The highest absorption effectiveness was obtained by ethanol extract and bracelets containing ethanol extract with the addition of CMC.

scholarly journals Maskulinisasi larva ikan nila (Oreochromis niloticus) melalui penggunaan madu dengan konsentrasi berbeda

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Soesanti S Odara ◽  
Juliaan Ch Watung ◽  
Hengky J Sinjal
Keyword(s):  
Experimental Design ◽  
Red Blood Cells ◽  
Analysis Of Variance ◽  
Oreochromis Niloticus ◽  
Blood Cells ◽  
Nile Tilapia ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to determine the effect of concentration of honey on masculinization of nile tilapia female. The study had four treatments, each with three replications. The experimental design used was completely randomized design (RAL).  Data was analyse using Analysis Of Variance (ANOVA) followed by LSD at 5% and 1%.  Broodstock was  soaked in 1 L of water with treatment A 0 mL (without honey), B (5 mL honey), C (10 mL honey), D (15 mL honey) for 10 hours. After soaking, fish was reared in pan for one month. The percentage of male was determine by comparing the length and width of the red blood cells under a microscope.  The results showed the the use of honey had significant effect on female masculinization.  The percentage of male larvae tended to increase with the increase of honey dose. The highest percentage of male was obtained in treatment D (15 ml) that was 80%.   Keywords: Honey, masculinization, larvae, Oreochromis niloticus

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

scholarly journals PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 169
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Virid Gibson ◽  
Alfiah Hayati
Keyword(s):  
Molecular Weight ◽  
Treatment Group ◽  
Mus Musculus ◽  
Control Group ◽  
Protein Profiles ◽  
Testosterone Levels ◽  
Randomized Design ◽  
Sds Page ◽  
Elisa Technique ◽  
Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of  15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of  60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

scholarly journals Promoting Color Brightness of Clown Loach (Chromobotia macracanthus Bleeker) by Mixing Red Spinach (Amaranthus tricolor L.) Powder with Feed Stuff

2020 ◽  
pp. 21-30
Author(s):  
Ayi Yustiati ◽  
Syakirah Imtinan Zurwana ◽  
Achmad Rizal ◽  
Yuli Andriani
Keyword(s):  
Research Method ◽  
Survival Rates ◽  
Optimal Dose ◽  
Primary Data ◽  
Test Results ◽  
Growth Data ◽  
Amaranthus Tricolor ◽  
Randomized Design ◽  
Powder Addition ◽  
Completely Randomized Design

The purpose of this research is to determine the optimal dose of the addition of red spinach powder to artificial feed on the brightness of clown loach. This research was conducted at Aquaculture Laboratory Building 4 Faculty of Fisheries and Marine Sciences of Universitas Padjadjaran from April to May 2020. This research method is experimental with a Completely Randomized Design consisting of four treatments and three replications. The red spinach powder addition treatment used 0%, 2%, 4%, and 6% of the feed amount. The parameters observed are color value as primary data by using Toca Color Finder, while the growth, survival rate, and water quality as support data. Color assessment results were analyzed using the Kruskal-Wallis test, if there were significant differences, Z test would be performed. Growth data and survival rates were analyzed using Analysis of Variance (ANOVA). Because the F test results were not significantly different, the Duncan test was not carried out. The results showed that the addition of  6% red spinach powder is the best treatment with an increased color brightness value of 5,63.

scholarly journals The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

2019 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Dora Dayu Rahma Turista ◽  
Eka Puspitasari
Keyword(s):  
Health Education ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Pathogenic Bacteria ◽  
Agar Plate ◽  
Blood Groups ◽  
Blood Agar Plate ◽  
Sheep Blood ◽  
Randomized Design

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.

scholarly journals PERBEDAAN PEMBERIAN LARUTAN VITAMIN B KOMPLEKS PADA TELUR TETAS TERHADAP HASIL TETAS AYAM KAMPUNG

2018 ◽  
Vol 12 (1) ◽  
pp. 31-51
Author(s):  
Aprilianto Bayu Arisandy ◽  
Agustina Widyasworo ◽  
Eddy Trijana Sudani
Keyword(s):  
Research Method ◽  
Domestic Chicken ◽  
Vitamin B ◽  
Research Material ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Chicken Eggs

ABSTRACT The research was conducted at Mrs. Sripurwanti's farm in Rejotangan Village, Rejotangan Sub-district, Tulung Agung Regency. The research was conducted from 1 March to 1 April 2017. The aim of the research to determine the difference in the solution of Vitamin B Complex to the results of domestic chicken include hatchability, mortality and hatching weight. Research material using selected 225 eggs of chicken eggs and Vitamin B Complex as many as 30 grains. The research method used is experiments with Completely Randomized Design (RAL). The study was divided into 5 treatments and 5 replications per repeat consisting of 9 eggs. Treatment consists of the addition of Vitamin B complex as much as 0, 45 mg, 90 mg, 135 mg, and 180 mg The results are expected can provide information about alternative moisture enhancer ingredients namely Vitamin B Komplek. The results showed that vitamin B complex was not significant (P> 0.05) to hatchability, live eggs and die 2nd candling , mortality, and hatching weight. Vitamin B complex has significant effect (P <0,05) on hatchability and mortality based on candling to 2. Result of the research can be concluded that giving Vitamin B Complex as much as 45 mg deserves to be additive addition of humidity because it can increase the percentage of hatchability by 15%

scholarly journals PENGARUH LOGAM BERAT TIMBAL TERHADAP PERTUMBUHAN ALGA Halimeda opuntia YANG DIKULTUR DALAM WADAH TERKONTROL

2020 ◽  
Vol 8 (3) ◽  
pp. 17
Author(s):  
Kristi Aji Sugiarto ◽  
Desy MH Mantiri ◽  
Nickson J Kawung ◽  
Rizald M Rompas ◽  
Sandra O Tilaar ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Content ◽  
Research Method ◽  
Lead Content ◽  
Randomized Design ◽  
Algae Growth ◽  
Completely Randomized Design ◽  
Over Time ◽  
Bioremediation Agent

Heavy metal is a very dangerous pollutant waste for it cannot be destroyed (non-degradable) and over time it will accumulate in the ocean. This study aims to determine the lead content in sediment, water and algal thallus as well as to determine the algae growth that has been treated with lead. The research method used was a completely randomized design (CRD) which consisted of 3 treatments, using different treatments for each sample. The highest metal content obtained in the sediment was 5,124 ppm, in the water was 0.120 ppm, and in the algae thallus was 4,816 ppm respectively. The results showed that Halimeda opuntia algae can grow even in an environment contaminated with lead heavy metal and can become a bioremediation agent by absorbing lead heavy metal. Keywords: Halimeda opuntia, Lead Heavy Metal, Algae Culture, Bioremediation             Logam berat merupakan salah satu limbah pencemar yang sangat berbahaya karena tidak dapat dihancurkan (non degradable) dan dapat terakumulasi di perairan laut. Penelitian ini bertujuan untuk mengetahui kandungan timbal pada sedimen, air dan thallus alga yang dikultur serta mengetahui pertumbuhan alga yang telah diberi perlakuan timbal. Metode penelitian mengunakan metode Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan, dengan menggunakan perlakuan yang berbeda pada tiap sampel dan kontrol. Kandungan logam tertinggi yang didapatkan pada sedimen yaitu sebesar 5,124 ppm, pada air sebesar 0,120 ppm, dan pada thallus alga sebesar 4,816 ppm. Hasil yang diperoleh yaitu alga Halimeda opuntia dapat bertumbuh walaupun dalam lingkungan yang tercemar logam berat timbal serta mampu menjadi agen bioremediasi dengan menyerap logam berat timbal. Kata kunci: Halimeda opuntia, Logam BeratTimbal, Kultur Alga, Bioremediasi

Sign in / Sign up

Export Citation Format

Share Document