scholarly journals Elicitation of Medicinal Plants In Vivo—Is It a Realistic Tool? The Effect of Methyl Jasmonate and Salicylic Acid on Lamiaceae Species

Horticulturae ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 5
Author(s):  
Wafae Kandoudi ◽  
Péter Radácsi ◽  
Beáta Gosztola ◽  
Éva Zámboriné Németh
Keyword(s):  
Antioxidant Activity ◽  
Salicylic Acid ◽  
Essential Oil ◽  
Methyl Jasmonate ◽  
Total Phenolics ◽  
Secondary Compounds ◽  
Stress Reactions ◽  
In Vivo Experiments ◽  
Scientific Experiments

Salicylic acid (SA) and methyl jasmonate (MeJa) are prominent phytohormones that are involved in stress reactions. Both compounds may influence the biosynthesis of secondary compounds; however, scientific experiments in vivo are rare and contradictive. This paper reports on a study on the elicitation of volatiles and total phenolics (TPC) by MeJa and SA. The subjects were four Lamiaceae species studied in open field conditions in Budapest (Hungary). According to the results, both elicitors provoked specific responses in each plant species depending on the dosage applied and the parameter studied; 2 mM of SA stimulated essential oil (EO) accumulation in marjoram and peppermint, while in hyssop 0.1 mM was optimal. MeJa proved to be effective only in marjoram and in basil. In marjoram, cis-sabinene hydrate was decreased and in hyssop, isopinocamphone was increased by both dosages of SA. In peppermint, pulegone content was reduced by 2 mM SA, but no significant change of the major components of basil EO was detected. SA was successful in increasing TPC and antioxidant activity (AC) in three of the experimental species, but not in hyssop. In marjoram, only 0.1 mM induced TPC and eventually AC, while in peppermint and basil both dosages of SA were effective. Optimalisation of the treatments is suggested in further in vivo experiments.

scholarly journals Preharvest Treatment of Methyl Jasmonate and Salicylic Acid Increase the Yield, Antioxidant Activity and GABA Content of Tomato

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2293
Author(s):  
Min Woo Baek ◽  
Han Ryul Choi ◽  
Lee Yun Jae ◽  
Ho-Min Kang ◽  
Ok-Hwan Lee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
Total Phenolics ◽  
Antioxidant Activities ◽  
Total Yield ◽  
Soluble Solids ◽  
Meja Treatment ◽  
Β Carotene

This study investigated the effect of preharvest treatment of 0.25 mM methyl jasmonate (MeJA) and 0.5 mM salicylic acid (SA) on quality parameters, most important secondary metabolites (lycopene, β-carotene, ascorbic acid, total phenolics, and flavonoids), antioxidant activities, and amino acid contents of ‘Kumato’ tomato. Treatments with MeJA and SA led to an increase of total yield per plant and were effective in maintaining the fruit quality attributes such as firmness, total soluble solids, titratable acidity, and Brix acid ratio at two maturity stages (S1 and S2). In addition, lycopene, β-carotene, ascorbic acid, total phenolics, and flavonoids were significantly higher in the treated fruit than in the control. Moreover, γ-aminobutyric acid (GABA), essential amino acids, and antioxidant activity increased due to MeJA and SA treatments at both stages, especially with MeJA treatment at S2. Overall results also showed the effectiveness of MeJA treatment than SA on improving the yield and nutritional quality of ‘Kumato’ tomato.

scholarly journals Assessment of Mint, Basil, and Lavender Essential Oil Vapor-Phase in Antifungal Protection and Lemon Fruit Quality

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1831 ◽  
Author(s):  
Renata M. Sumalan ◽  
Raufdzhon Kuganov ◽  
Diana Obistioiu ◽  
Iuliana Popescu ◽  
Isidora Radulov ◽  
...  
Keyword(s):  
Essential Oil ◽  
Postharvest Quality ◽  
Quality Properties ◽  
Chemical Treatments ◽  
In Vivo Experiments ◽  
Ic50 Values ◽  
Set Up

There is an increasing interest in developing natural methods to replace the current chemicals used for maintaining postharvest quality of citrus fruits. The essential oil antifungal activity of mint (MEO), basil (BEO), and lavender (LEO) acting as the vapor-phases was tested against Penicillium digitatum. The minimum doses with fungistatic and fungicidal effect, in vitro, acting as the vapor-phases, were set up. The minimum fungicidal dose was 300 μL for BEO and 350 μL LEO, while for MEO only minimal dose with fungistatic effect was reached. The IC50 values were calculated and used (v/v) for testing preservation of lemon fruits, in close space enriched in vapor oil. For this purpose, the following two independent in vivo experiments were carried out: experiment 1, inoculated lemons with P. digitatum stored without chemical treatments 7 days, at 22 ± 2 °C, at two concentrations (C1—IC50 equivalent; C2—half of C1); and experiment 2, the non-inoculated lemons kept under the same conditions and concentrations of EO vapor served to evaluate the lemon quality properties. The results showed that antifungal protective effect was provided in the order of LEO-C1 > BEO-C1 > MEO-C1 > BEO-C2 > MEO-C2 > LEO-C2. The quality indicators like weight loss, pH, and firmness were not negatively influenced.

scholarly journals Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil

2015 ◽  
Vol 87 (2 suppl) ◽  
pp. 1397-1408 ◽  
Author(s):  
GABRIELA L. DA SILVA ◽  
CAROLINA LUFT ◽  
ADROALDO LUNARDELLI ◽  
ROBSON H. AMARAL ◽  
DENIZAR A. DA SILVA MELO ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Therapeutic Potential ◽  
Positive Control ◽  
Croton Oil ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Pre Treatment ◽  
Dose Dependent

Several studies have investigated the antinociceptive, immunomodulatory and anti-inflammatory properties of compounds found in the lavender essential oil (LEO), however to date, there is still lack of substantial data. The objective of this study was to assess the antioxidant, anti-inflammatory and antinociceptive effects of lavender essential oil. The 1,1-diphenyl-2-picrylhydrazyl radical decolorization assay was used for antioxidant activity evaluation. The anti-inflammatory activity was tested using two models of acute inflammation: carrageenan-induced pleurisy and croton oil-induced ear edema. The antinociceptive activity was tested using the pain model induced by formalin. LEO has antioxidant activity, which is dose-dependent response. The inflammatory response evoked by carrageenan and by croton oil was reduced through the pre-treatment of animals with LEO. In the pleurisy model, the drug used as positive control, dexamethasone, was more efficacious. However, in the ear swelling, the antiedematogenic effect of the oil was similar to that observed for dexamethasone. In the formalin test, LEO consistently inhibited spontaneous nociception and presented a similar effect to that of tramadol. The results of this study reveal (in vivo) the analgesic and anti-inflammatory activities of LEO and demonstrates its important therapeutic potential.

scholarly journals LOWERING URIC ACID EFFICACY TEST OF THE COMBINED EXTRACT OF UNCARIA GAMBIR (HUNTER) ROXB. AND CAESALPINIA SAPPAN L. IN VIVO AND IN VITRO

2018 ◽  
Vol 11 (8) ◽  
pp. 166
Author(s):  
Sri Ningsih ◽  
Fahri Fahrudin
Keyword(s):  
Antioxidant Activity ◽  
Uric Acid ◽  
Inhibitory Activity ◽  
Positive Control ◽  
Polyphenol Content ◽  
Caesalpinia Sappan ◽  
In Vivo Experiments ◽  
A Value

 Objective: Hyperuricemia (high uric acid levels) prevalence increased year by year. This study was aimed to elaborate the in vitro xanthine oxidase (XO) inhibitory activity and in vivo lowering hyperuricemic effect of Uncaria gambir (Hunter) Roxb) (gambir), Caesalpinia sappan L. (secang) and the combined extract of secang and gambir (formulae extract [FE]).Methods: Gambir and secang extracts were prepared by maceration with ethanol and FE was the proportioned combination of these two extracts. XO inhibitory activity was determined by measuring the formation of uric acid in the xanthine/XO system in vitro using allopurinol as a positive control at 100 ug/mL. Antioxidant activity was by 1,1-diphenyl-2-picrylhydrazyl radical reducing methods. The in vivo experiments were conducted in the oxonate-induced hyperuricemia rat model, in which FE was gavaged p.o. at the arrange dose of 75, 150, and 300 mg/kg bw for 2 weeks. Polyphenol content was measured using Folin–Ciocalteu reagent spectrophotometrically.Results: The XO inhibitory activity of FE was 80% of allopurinol, while secang and gambir were 98% and 50%, respectively. The strength was appropriate to the total polyphenol content, in which it decreased in the order of secang (99%) > FE (86%) > gambir (46%). Furthermore, FE at all tested doses was able to decrease uric acid levels. FE also demonstrated antioxidant activity with a value of 74% relative to Vitamin C at 4 ug/mL.Conclusion: These studies could be concluded that FE exhibited the ability to decrease uric acid level so that it was potential to be developed further as a uric acid-lowering agent.

scholarly journals Effect of Salinity on Morphological Characteristics, Biochemical Accumulation, and Essential Oil Yield and Composition in Black Horehound (Ballota nigra L.)

2021 ◽  
Author(s):  
Mehdi Younessi Mehdikhanlou ◽  
Zahra Dibazarnia ◽  
Shahin Oustan ◽  
Vinson Teniyah ◽  
Ramesh Katam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
In Vitro Culture ◽  
Salinity Stress ◽  
Salt Concentration ◽  
Total Phenolics ◽  
Morphological Characteristics ◽  
Culture Conditions ◽  
Total Flavonoids

Black horehound (Ballota nigra L.) is one of the important medicinal plants, which is a rich source of health-promoting essential oils. Salinity stress affects plant development and alters the quality and quantity of plants extracts and their composition. This study was aimed to investigate the effect of salinity on morphological, physiological characteristics, and secondary metabolites of B. nigra under greenhouse, and in vitro culture conditions. The plants were treated with different concentrations of NaCl (25, 50, 75, 100 mM) and fresh and dry weight of leaf and stem were measured as well as morphological characteristics of the plant. Plant growth was reduced with the increased salinity concentrations. The results showed that all growth-related traits and SPAD were decreased both in vivo and in vitro. Additionally, increased salt concentration affected the cell membrane integrity. Total phenolics content of plants growing in the greenhouse, increased by 21% at 50 mM NaCl, but at higher stress levels (100 mM NaCl), the amounts were decreased significantly. Total flavonoids contents followed similar patterns, with a slight difference. In addition, the maximum and minimum total phenolics contents of plants growing under in vitro condition were observed at 50 mM NaCl and control treatments, respectively. Increasing the salt concentration significantly affected the total flavonoids content, and as a result, the highest amount was observed in 50 and 75 mM NaCl treatments. Antioxidant activity was also measured. Among the NaCl treatments, the highest DPPH scavenging activities (IC50) under greenhouse and in vitro conditions were detected at 50 mM and 25 mM concentrations, respectively. In general, based on the results, with increasing the salinity level to 75 mM, the activities of CAT and APX were significantly upregulated in both greenhouse and in vitro culture conditions. A correlation between total phenolics and flavonoids contents as well as antioxidant activity were obtained. With shifting salinity stress, the type and the amount of the identified essential oil compounds changed. Compounds such as styrene, tridecanol, germacrene-D, beta-Ionone, beta-bisabolene, and caryophyllene oxide increased compared to the controlled treatment.

Antifungal, Anti-aflatoxigenic, Antioxidant Activity and in vivo Efficacy of Essential Oil of the Aerial Parts of Thymus capitatus (L.) Hoffmanns & Link

2018 ◽  
Vol 17 (6) ◽  
pp. 299-309 ◽  
Author(s):  
Y. Ben Miri ◽  
D. Djenane
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Aspergillus Flavus ◽  
Good Alternative ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Human Beings ◽  
Thymus Capitatus ◽  
Aflatoxin B

Aspergillus flavus has been reported to be the most common fungus used as food by human beings. This fungus may have the potential to produce aflatoxin B1 (AFB1), which is reported as being the most hepatotoxic, teratogenic, mutagenic, and immunosuppressive to humans and other livestock. Therefore, the objective of this work was to study the chemical profile of Thymus capitatus essential oil (EO) and the inhibition of growth of Aspergillus flavus E73 and AFB1 production by the EO. The antioxidant activity and phytotoxicity were also evaluated. The gas chromatography– mass spectrometry (GC–MS) analysis showed that the major components of Thymus capitatus EO were thymol (25.82%), linalool (23.40%), geraniol (14.22%), pcymen- 3-ol (8.93%), and p-cymene (6.76%). The results showed that the EO could inhibit the growth of Aspergillus flavus E73 in the range of 40.72 to 87.00%. The minimal inhibitory concentration (MIC) of Thymus capitatus EO against Aspergillus flavus E73 was found to be at 1.00 mg/ml. The oil revealed complete inhibition of dry mycelium weight and AFB1 production at 1.00 mg/ml. The EO revealed a broad spectrum of fungitoxicity against some fungi. The antioxidant activity was also assessed where IC50 (when initial concentration is reduced to half) and β- carotene/linoleic acid inhibition percentage of Thymus capitatus EO were 619.16 ± 3.94 μg/ml and 65.55%, respectively, while the total phenolic content was 21.45 μg/mg. The EO showed non-phytotoxicity on two varieties of wheat seeds. These findings demonstrated that EO could be good alternative to protect food.

scholarly journals Preharvest Methyl Jasmonate Treatment Increased the Antioxidant Activity and Glucosinolate Contents of Hydroponically Grown Pak Choi

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 131
Author(s):  
Min Woo Baek ◽  
Han Ryul Choi ◽  
Tifsehit Solomon ◽  
Cheon Soon Jeong ◽  
Ok-Hwan Lee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methyl Jasmonate ◽  
Total Phenolics ◽  
Trolox Equivalent Antioxidant Capacity ◽  
World Population ◽  
Pak Choi ◽  
Phytochemical Composition ◽  
Meja Treatment ◽  
Vertical Hydroponics ◽  
Hydroponically Grown

Vertical hydroponics farming has emerged as an alternative solution to feed the continuously growing world population. Additionally, recent studies reported that the exogenous treatments of jasmonic acid influence the phytochemical composition of Brassicaceae. We conducted this study to determine the effect of preharvest methyl jasmonate (MeJA) treatment on the phytochemical composition and antioxidant activities of soil- and hydroponically grown pak choi. An aqueous solution of 0.5-mM MeJA was sprayed to saturation on the aerial plant part three days before harvest. The harvested pak choi was freeze-dried and then powdered to measure the antioxidant activity and the contents of chlorophylls (Chls), total phenolics and flavonoids, and glucosinolates (GSLs). The overall results revealed that pak choi grown in vertical hydroponics had higher total Chls and total phenolics than those grown in soil in the greenhouse, regardless of MeJA treatment. Nevertheless, the GSLs content and total flavonoids increased significantly due to MeJA treatment in both growing systems, and the highest values were recorded in hydroponically grown MeJA-treated pak choi. Similarly, the 2, 2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, Trolox-equivalent antioxidant capacity (ABTS), oxygen radical absorbance capacity (ORAC), and ferric-reducing antioxidant power (FRAP) were highest in hydroponically grown MeJA-treated pak choi. Taken together, the preharvest foliar treatment of MeJA can be used to improve the phytochemical composition of pak choi grown in both growing systems. Interestingly, the results strongly support the use of MeJA treatment in the vertical hydroponics growing system compared to the conventional growing system in the soil. This indicates that supplementing the vertical hydroponic growing system with preharvest MeJA treatment could be the best option to improve both the yield per square meter and the quality of pak choi. Besides, MeJA-treated pak choi could be used as a value-added horticultural commodity, as its antioxidant activity increased after treatment. Moreover, after further studies, MeJA could also be applied to other Brassica vegetables to improve their GSL contents and antioxidant properties.

scholarly journals Antioxidant Property and Bactericidal Activity of Linum usitatissimum Seed Essential Oil Nanoemulsion (LSEO-NE) on Staphylococcus aureus

2020 ◽  
Vol 7 (2) ◽  
Author(s):  
Roghaye Keykhasalar ◽  
Masoud Homayouni Tabrizi ◽  
Pouran Ardalan
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Bactericidal Activity ◽  
Linum Usitatissimum ◽  
Bacterial Infections ◽  
Antioxidant Property ◽  
In Vivo Studies ◽  
Force Microscopy

Background: Linum usitatissimum Seed Essential Oil (LSEO) as an efficient antimicrobial compound contains various types of phytochemicals, such as lignans and phenols. Objectives: In the current study, we produced LSEO nanoemulsion (LSEO-NE) to study its antioxidant capacity and bactericidal activity against Staphylococcus aureus. Methods: The LSEO-NE was produced using the ultrasonication method and characterized by Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS), and Scanning Electron Microscopy (SEM). Then, we measured its antioxidant activity utilizing both ABTS and DPPH tests at four different LSEO-NE concentrations (200, 600, 800, and 1000 µg/mL) compared to glutathione. Finally, we evaluated its bactericidal activity on S. aureus by studying Antibiotic Susceptibility Testing (AST) using LSEO-NE-smeared discs compared to non-smeared and kanamycin discs. Results: The 67.3 nm droplets of LSEO-NE with PDI of 0.452 exhibited strong antioxidant activity, similar to glutathione, in both ABTS (IC50 = 350 µg/mL) and DPPH (IC50 = 235 µg/mL) tests. Moreover, the AST results revealed the significant sensitivity of S. aureus to LSEO-NE-smeared discs when compared to non-smeared and kanamycin discs. Conclusions: According to the results, LSEO-NE can be applied as a safe, natural, and effective antibiotic for bacterial infections caused by S. aureus in most organs, such as the respiratory system and skin. However, further in vivo studies are required to evaluate the LSEO-NE antibacterial efficiency against other pathogenic S. aureus strains.

Sign in / Sign up

Export Citation Format

Share Document