scholarly journals Evaluation of cashew (Anacardium occidentale) accessions diversity of in large pools of production in Cameroon and their germ performance to increase productivity

2020 ◽  
Vol 46 (2) ◽  
pp. 8212-8225
Author(s):  
Chantal MADOU ◽  
SALI Bourou ◽  
AWE DJONGMO Victor ◽  
Abraham NOME
Keyword(s):  
Coat Color ◽  
Germination Rate ◽  
Morphological Characteristics ◽  
Fruit Weight ◽  
Plain Water ◽  
P Value ◽  
Significant Difference ◽  
Northern Zone ◽  
Plant Sciences ◽  
Production Areas

The sector faces many difficulties, in particular the mastery of the different varieties and the germination aptitudes by the planters. This study is therefore a contribution to the characterization of the diversity of cashew accessions in the northern zone of Cameroon as well as their germination performance. Physical characteristics such as: fruit weight, apple length, apple weight, nut weight and length, and seed coat color were assessed in this study.It appears that the morphological characteristics of the fruit vary according to production areas. Analysis of variance confirmed a significant difference in the morphological characteristics of cashew fruit between the four study areas (P-value <0.05), except the ratio of the weight apple and nut. The fruits of Ngaoundéré area have the best characteristics in terms of fruit weight (140.66 ± 14.24 g), length of head (75.11 ± 5.60 mm), the length of nuts (41, 63 ± 1.34 mm), apple weight (126.87 ± 13.99 mm) and nuts weight (13.78 ± 0.60 mm). The method of soaking nuts for 48 hours in plain water is a technique that provides a waiting time of 8 to 15 days before the start of germination, with a germination period of between 11-23 days and a germination rate of 90 to 100%. The results recorded showed a fairly good germination capacity of the seeds resulting from the various pretreatments. The mastery of these germination techniques is essential to improve the production of nurseries. RESUME L’anacardier occupe une place importante dans l’économie du Cameroun. La filière est confrontée cependant à un certain nombre de difficultés, notamment la méconnaissance des caractéristiques des différentes variétés par les producteurs. Cette étude est donc une contribution à la caractérisation des anacardiers présents dans la zone septentrionale du Cameroun. Les caractéristiques morphologiques (poids du fruit, longueur de la pomme, poids de la pomme, poids et longueur de la noix) ont été évaluées. Il ressort que ces caractéristiques varient en fonction des bassins de productions. L’analyse de variance atteste Madou et al., 2020 Journal of Animal & Plant Sciences (J.Anim.Plant Sci. ISSN 2071-7024) Vol.46 (2): 8212-8225 https://doi.org/10.35759/JAnmPlSci.v46-2.5 8213 une différence significative des caractéristiques morphologiques des fruits d’anacardes entre les quatre zones d’études (P-value<0,05), excepté le rapport entre le poids de la pomme et de la noix. Les fruits de la zone Ngaoundéré présentent les meilleures caractéristiques en termes du poids fruits (140,66 ± 14,24 g), de la longueur de pomme (75,11 ± 5,60 mm), de la longueur de noix (41,63 ± 1,34 mm), du poids de pomme (126,87 ± 13,99 mm) et du poids de noix (13,78 ± 0,60 mm). La méthode de trempage des noix pendant 48heurs dans de l’eau ordinaire est une technique qui permet d’obtenir une durée d’attente allant de 8 à 15 jours avant le début de la germination, avec un délai de germination variant entre 11 à 23 jours et un taux germination de 90 à 100%. Les résultats obtenus ont montré une assez bonne aptitude à la germination des semences issues des différents prétraitements. La connaissance de ces techniques est fondamentale pour tout programme d'amélioration de la production des pépinières.

scholarly journals Comparative studies of Echinometra mathaei species complex (Echinoidea: Camarodonta: Echinometridae) from two sites in Western Visayas, Philippines (Taklong Island, Guimaras and Nabas, Aklan)

2019 ◽  
Author(s):  
Joseph Ricky Tamayo ◽  
Maria Celia D Malay
Keyword(s):  
Species Complex ◽  
Marine Reserve ◽  
Morphological Characteristics ◽  
The Philippines ◽  
P Value ◽  
Rocky Shores ◽  
Significant Difference ◽  
Coral Communities ◽  
Echinometra Mathaei ◽  
The Mean

Echinometra mathaei is a species complex with its constituent reproductively-isolated species informally called A, B, C and D based on studies done in Okinawa and the Red Sea. Little research has been done on this genus, and to our knowledge no studies have been done on the E. mathaei complex in the Philippines. To help clarify species delineations in the E. mathaei complex, a comparative study was done between two localities in Western Visayas, Philippines: the Taklong Island National Marine Reserve, in Nueva Valencia, Guimaras and Barangay Unidos in Nabas, Aklan. Morphological characteristics (spine color, milled rings, and skin around the peristome) and tubefeet and gonad spicules were observed. Two or possibly three species of Echinometra were found in the two sites based on their morphology and spicules, namely: Echinometra sp. A, Echinometra sp. C, and Echinometra affinity C, which resembles sp. C but differs in the milled rings and gonad spicules. Echinometra sp. C and E. affinity C cannot be distinguished on the basis of field-visible characters, thus the two morphs are referred to as Echinometra VC for the purpose of field surveys. Echinometra VC and Echinometra sp. A exhibited differences in abundance (VC was much more common) and microhabitat (VC was restricted to rocky shores and never observed in coral communities). To study the abundance and distribution of Echinometra VC, 50 m by 2 m belt transects were surveyed along the rocky shores of both sites: two parallel transects (at 0 m and at 0.9 m) and a perpendicular transect (only in Nabas), each with three replicates. The transect data showed that the mean densities for 0 m and 0.9 m in Nabas are significantly higher in Taklong yielding p-values of 0.001 and 0.002, respectively, when analyzed using t-test.Of the two sites, only Nabas showed a significant difference between the mean densities at 0 m and 0.9 m, with the mean density at 0.9 m significantly higher than that of 0 m yielding a p-value of 0.02 when analyzed using two-sample t-test. A Poisson regression on the perpendicular transect data from Nabas showed a trend of increasing Echinometra density with increasing distance from the shore. In the future, DNA barcoding and cross-fertilization studies should be performed in order to confirm the species of Echinometra observed. Many factors can affect the density and distribution of Echinometra, so further studies must be conducted to explain observed differences in their distribution and abundance.

scholarly journals The Potential of Billygoat Weed Leaf Extract (Ageratum Conyzoides) in Increasing Platelet Counts of Male Mice (Mus Musculus)

2020 ◽  
Vol 11 (1) ◽  
pp. 51-57
Author(s):  
Yessy Velina ◽  
Nirva Diana ◽  
Aulia Annisa
Keyword(s):  
Platelet Count ◽  
Positive Control ◽  
Negative Control ◽  
Plain Water ◽  
P Value ◽  
Male Mice ◽  
Ageratum Conyzoides ◽  
Platelet Counts ◽  
Randomized Design ◽  
Significant Difference

Billygoat weed (Ageratum conyzoides) is mainly used to treat wounds and fever. People also do not understand the use of these leaves in the treatment to increase the platelet counts. The objectives of this research Objectives were to test whether the Billygoat Weed’s leaves can increase platelet count of male mice and to find out what is the most effective dose of the Billygoat Weed on the platelet count of male mice. This research was done by employing the true experimental approach with the completely randomized design which consisted of 5 treatments, namely the negative control (plain water), the positive control (acetylsalicylic acid), 1.68 mg/kgBW, 2.52 mg/kgBW, and 3.36 mg/kgBW on five mice in each treatment. The increase in the platelet counts, based on the results of One-Way ANOVA with a p-value of 0,0004, the Billygoat Weed extract was able to increase the platelet counts. Through further testing, the smallest significant difference showed that a dose of 3.36 mg/kgBW was effectively able to increase the platelet counts of male mice with an average of 11140.80/. Billygoat weed (Ageratum conyzoides) is mainly used to treat wounds and fever. People also do not understand the use of these leaves in the treatment to increase the platelet counts. The objectives of this research Objectives were to test whether the Billygoat Weed’s leaves can increase platelet count of male mice and to find out what is the most effective dose of the Billygoat Weed on the platelet count of male mice. This research was done by employing the true experimental approach with the completely randomized design which consisted of 5 treatments, namely the negative control (plain water), the positive control (acetylsalicylic acid), 1.68 mg/kgBW, 2.52 mg/kgBW, and 3.36 mg/kgBW on five mice in each treatment. The increase in the platelet counts, based on the results of One-Way ANOVA with a p-value of 0,0004, the Billygoat Weed extract was able to increase the platelet counts. Through further testing, the smallest significant difference showed that a dose of 3.36 mg/kgBW was effectively able to increase the platelet counts of male mice with an average of 11140.80/𝑚𝑚3.

scholarly journals Comparative studies of Echinometra mathaei species complex (Echinoidea: Camarodonta: Echinometridae) from two sites in Western Visayas, Philippines (Taklong Island, Guimaras and Nabas, Aklan)

2019 ◽  
Author(s):  
Joseph Ricky Tamayo ◽  
Maria Celia D Malay
Keyword(s):  
Species Complex ◽  
Marine Reserve ◽  
Morphological Characteristics ◽  
The Philippines ◽  
P Value ◽  
Rocky Shores ◽  
Significant Difference ◽  
Coral Communities ◽  
Echinometra Mathaei ◽  
The Mean

Echinometra mathaei is a species complex with its constituent reproductively-isolated species informally called A, B, C and D based on studies done in Okinawa and the Red Sea. Little research has been done on this genus, and to our knowledge no studies have been done on the E. mathaei complex in the Philippines. To help clarify species delineations in the E. mathaei complex, a comparative study was done between two localities in Western Visayas, Philippines: the Taklong Island National Marine Reserve, in Nueva Valencia, Guimaras and Barangay Unidos in Nabas, Aklan. Morphological characteristics (spine color, milled rings, and skin around the peristome) and tubefeet and gonad spicules were observed. Two or possibly three species of Echinometra were found in the two sites based on their morphology and spicules, namely: Echinometra sp. A, Echinometra sp. C, and Echinometra affinity C, which resembles sp. C but differs in the milled rings and gonad spicules. Echinometra sp. C and E. affinity C cannot be distinguished on the basis of field-visible characters, thus the two morphs are referred to as Echinometra VC for the purpose of field surveys. Echinometra VC and Echinometra sp. A exhibited differences in abundance (VC was much more common) and microhabitat (VC was restricted to rocky shores and never observed in coral communities). To study the abundance and distribution of Echinometra VC, 50 m by 2 m belt transects were surveyed along the rocky shores of both sites: two parallel transects (at 0 m and at 0.9 m) and a perpendicular transect (only in Nabas), each with three replicates. The transect data showed that the mean densities for 0 m and 0.9 m in Nabas are significantly higher in Taklong yielding p-values of 0.001 and 0.002, respectively, when analyzed using t-test.Of the two sites, only Nabas showed a significant difference between the mean densities at 0 m and 0.9 m, with the mean density at 0.9 m significantly higher than that of 0 m yielding a p-value of 0.02 when analyzed using two-sample t-test. A Poisson regression on the perpendicular transect data from Nabas showed a trend of increasing Echinometra density with increasing distance from the shore. In the future, DNA barcoding and cross-fertilization studies should be performed in order to confirm the species of Echinometra observed. Many factors can affect the density and distribution of Echinometra, so further studies must be conducted to explain observed differences in their distribution and abundance.

scholarly journals Germination, Growth and Development of Mangifera indica L. Varieties Used as Rootstocks on Different Substrates

2020 ◽  
Vol 4 (3) ◽  
pp. 435-455
Author(s):  
Ousmane NDIAYE ◽  
Boubacar CAMARA ◽  
Antoine SAMBOU ◽  
Saliou NDIAYE
Keyword(s):  
Forest Soil ◽  
Growth And Development ◽  
Growth Parameters ◽  
Germination Rate ◽  
Mangifera Indica ◽  
Principal Component ◽  
Anacardium Occidentale ◽  
P Value ◽  
Significant Difference ◽  
Khaya Senegalensis

Mango (Mangifera indica L.) is a tree of the family Anacardiaceae, native to southern Asia, widely cultivated in tropical countries for its fruit, the mango. The mango production has achieved worldwide usefulness as a fruit, a medicinal use and a economic importance.  Despite the importance of Mangifera indica L. Senegal is facing to the loss of the mango productivity and the decline of mango tree diversity. It is therefore important to improve the selection and the genetically improvement of the rootstocks. This study aims to test the germination, growth and development of Mangifera indica L varieties locally known as Diourou, Siera Leone, Pince and Kouloubadaseky on three types of substrates. The substrates used were three potting of forest soil of Anacardium occidentale, Mangifera indica and Khaya senegalensis. Thus, the nuts of the different varieties were sown in sheaths filled with pure potting forest soil from the different substrates (Anacardium occidentale, Mangifera indica and Khaya senegalensis. A germination test and a follow-up of seedlings on different substrates was carried out in the nursery to determine the effect of substrates on growth parameters. The germination dynamics of the three varieties reveals two peaks the first at the 24th day for Diourou and Kouloubadaseky varieties and another the 34th day for the Siera Leone and Pince varieties on all substrates. And the overall germination rate is 63.22% ± 1.88. The results showed that the number of stems per seed depends on the variety but not on the substrates. The Principal Component Analysis showed significative correlations between parameters such as the number of leaves, the height and the diameter at the crown of the stem. The analysis of variance showed a significant difference (P-value˂0.05) between varieties and according to the substrate on the parameters studied. All this information can be basic use for growers when choosing the varieties and type of substrate for mango rootstock production in their nurseries.

scholarly journals Relationship between the Characteristics of Bread Wheat Grains, Storage Time and Germination

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 35
Author(s):  
Dmitry A. Afonnikov ◽  
Evgenii G. Komyshev ◽  
Vadim M. Efimov ◽  
Mikhail A. Genaev ◽  
Vasily S. Koval ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Storage Time ◽  
Coat Color ◽  
Recombinant Inbred Lines ◽  
Germination Rate ◽  
Seed Viability ◽  
Morphological Characteristics ◽  
Correlation Coefficients ◽  
Seed Storage ◽  
Seed Traits

Seed storage is important to farmers, breeders and for germplasm preservation. During storage, seeds accumulate damage at the structural and metabolic level, which disrupt their function and reduce resistance to adverse external conditions. In this regard, issues related to seed aging prove to be relevant for maintaining the viability of genetic collections. We analyzed morphological characteristics of grains and their coat color for 44 recombinant inbred lines (RILs) of bread wheat grown in four different seasons, 2003, 2004, 2009 and 2014. Our investigations were performed in 2020. For 19 RILs from the same seasons germination was evaluated. Our results demonstrate that genotype significantly affects the variability of all seed traits, and the year of harvesting affects about 80% of them (including all the traits of shape and size). To identify the trend between changes in grain characteristics and harvesting year, we estimated correlation coefficients between them. No significant trend was detected for the grain shape/size traits, while 90% of the color traits demonstrated such a trend. The most significant negative correlations were found between the harvesting year and the traits of grain redness: the greater the storage time, the more intensive is red color component for the grains. At the same time, it was shown that grains of longer storage time (earlier harvesting year) have lighter coat. Analysis of linear correlations between germination of wheat seeds of different genotypes and harvesting years and their seed traits revealed a negative linear relationship between the red component of coat color and germination: the redder the grains, the lower their germination rate. The results obtained demonstrate manifestations of metabolic changes in the coat of grains associated with storage time and their relationship with a decrease of seed viability.

scholarly journals Comparative studies of Echinometra mathaei species complex (Echinoidea: Camarodonta: Echinometridae) from two sites in Western Visayas, Philippines (Taklong Island, Guimaras and Nabas, Aklan)

2019 ◽  
Author(s):  
Joseph Ricky Tamayo ◽  
Maria Celia D Malay
Keyword(s):  
Species Complex ◽  
Marine Reserve ◽  
Morphological Characteristics ◽  
The Philippines ◽  
P Value ◽  
Rocky Shores ◽  
Significant Difference ◽  
Coral Communities ◽  
Echinometra Mathaei ◽  
The Mean

Echinometra mathaei is a species complex with its constituent reproductively-isolated species informally called A, B, C and D based on studies done in Okinawa and the Red Sea. Little research has been done on this genus, and to our knowledge no studies have been done on the E. mathaei complex in the Philippines. To help clarify species delineations in the E. mathaei complex, a comparative study was done between two localities in Western Visayas, Philippines: the Taklong Island National Marine Reserve, in Nueva Valencia, Guimaras and Barangay Unidos in Nabas, Aklan. Morphological characteristics (spine color, milled rings, and skin around the peristome) and tubefeet and gonad spicules were observed. Two or possibly three species of Echinometra were found in the two sites based on their morphology and spicules, namely: Echinometra sp. A, Echinometra sp. C, and Echinometra affinity C, which resembles sp. C but differs in the milled rings and gonad spicules. Echinometra sp. C and E. affinity C cannot be distinguished on the basis of field-visible characters, thus the two morphs are referred to as Echinometra VC for the purpose of field surveys. Echinometra VC and Echinometra sp. A exhibited differences in abundance (VC was much more common) and microhabitat (VC was restricted to rocky shores and never observed in coral communities). To study the abundance and distribution of Echinometra VC, 50 m by 2 m belt transects were surveyed along the rocky shores of both sites: two parallel transects (at 0 m and at 0.9 m) and a perpendicular transect (only in Nabas), each with three replicates. The transect data showed that the mean densities for 0 m and 0.9 m in Nabas are significantly higher in Taklong yielding p-values of 0.001 and 0.002, respectively, when analyzed using t-test.Of the two sites, only Nabas showed a significant difference between the mean densities at 0 m and 0.9 m, with the mean density at 0.9 m significantly higher than that of 0 m yielding a p-value of 0.02 when analyzed using two-sample t-test. A Poisson regression on the perpendicular transect data from Nabas showed a trend of increasing Echinometra density with increasing distance from the shore. In the future, DNA barcoding and cross-fertilization studies should be performed in order to confirm the species of Echinometra observed. Many factors can affect the density and distribution of Echinometra, so further studies must be conducted to explain observed differences in their distribution and abundance.

PENENTUAN NILAI PARAMETER LIPOFILITAS SENYAWA 4-KLOROBENZOILTIOUREA DAN UJI POTENSIASI TERHADAP TIOPENTAL PADA MENCIT PUTIH (Mus musculus)

2019 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Dini Kesuma
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Electronic Properties ◽  
Mus Musculus ◽  
Log P ◽  
P Value ◽  
Lead Compounds ◽  
Significant Difference ◽  
Central Nervous System Depressant ◽  
The Central Nervous System

Synthesis of the 4-chlorobenzoylthiourea compound was carried out by acylating thiourea with 4-chlorobenzoyl chloride. The 4-chlorobenzoylthiourea compound  will increase the lipophilic and the electronic properties other than the lead compounds of benzoylthiourea in order to, by expectation, raise the central nervous system depressant as well. The lipophilic would affect the ability of the compounds in penetrating biological membranes, which is highly dependent on the solubility of the drug within lipid/water. Log P is the most common method used in determining the parameter value. This experiment was to mix two dissolvents (octanol and water) which are immissible. The both levels of the compounds were carefully observed by a spectrophotometer UV-Vis. From the test, the result of log P value of the 4-chlorobenzoylthiourea compound was 2.32, while the theoretical log P value of the compounds, by using the π Hansch-Fujita method is 1.62 and the f Rekker-Mannhold method is 2.225. Consequently, the result of the test shows that there is a significant difference between the progress experiment and both theoretical log P methods. Moreover, in the test of the central nervous system depressant through the potentiation test to thiopental using mice indicates that the 4-chlorobenzoylthiourea compound have potentiation effects to thiopental compared to the lead compounds of benzoylthiourea.

Employing Personal Reading Logs through Short Stories to Enhance Students’ L2 Reading Comprehension Achievement

2019 ◽  
Vol 3 (2) ◽  
pp. 1
Author(s):  
Eliyas Sulaiman Mohandas ◽  
Nik Mastura Nik Ismail Azlan ◽  
Salwa Othman ◽  
Muhammad Aizat Azhari
Keyword(s):  
Short Stories ◽  
Reading Comprehension ◽  
P Value ◽  
L2 Reading ◽  
Reading Score ◽  
Paired Samples ◽  
Significant Difference ◽  
Post Test ◽  
The Mean ◽  
L2 Reading Comprehension

This study aims to investigate whether the use of six selected short stories throughout the duration of a 14-week course could enhance students’ reading comprehension achievement at the end of the semester. Out of the six short stories read, three were chosen as in-class assignments known as ‘Personal Reading Logs’ (hereafter, PRLs). One group of semester two Diploma students taking a reading skills course was selected through a convenience sampling method. A pre-test was conducted by having the students answer a past semester reading quiz of which the results would then be compared to their post-test (final reading exam) results. A paired samples t-test revealed no significant difference in the reading scores of the pre-test and the post-test, t (17) = -.265, p > .05. Since the p-value was bigger than 0.05, this indicated that the mean reading score of the post-test (M = 50.556) was not significantly higher than the mean reading score of the pre-test (M = 49.722). Therefore, the null hypothesis which stated that there was no difference in the mean score of the pre-test and post-test was retained. Overall, the result refuted the findings of other studies promoting the effectiveness of using short stories to enhance L2 reading comprehension achievement.

THE ANALYSIS OF NURSING STUDENT SOFT SKILLS DEVELOPMENT BETWEEN LECTURE AND PROBLEM BASED LEARNING APPROACHES 2011

2018 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Hilman Syarif
Keyword(s):  
Nursing Students ◽  
Nursing Student ◽  
Soft Skills ◽  
Comparative Method ◽  
P Value ◽  
Learning Approaches ◽  
Soft Skill ◽  
Student Centered Learning ◽  
Hard Skills ◽  
Significant Difference

Introduction: Student Centered Learning (SCL) is an effective method to develop student's soft skills and hard skills which are very important to support their successful carrier later. This research was conducted to identify the differences of developed soft skills between students who learn with PBL method and lecturing method. Methods: Descriptive comparative method was used in this study. The samples consisted of 15 students who learned with PBL method and 15 students who learned with lecturing method. The sample for this research was selected by random sampling method. Results: The result showed that the average of student's soft skills score in PBL method was 122.63, while student's soft skills score in lecturing method was 116.27. Discussion & Conclusion: There was significant difference of student's soft skills in PBL method and lecturing method (p value = 0.038; α = 0.05). This study recommends nursing program management, faculty of medicine at the University of Syiah Kuala keeps PBL method running and develops other methods which facilitate hard skills and soft skills are development. Keywords: nursing students, soft skill, PBL

Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit

2018 ◽  
Vol 1 (2) ◽  
pp. 114
Author(s):  
Wahdaniah Wahdaniah ◽  
Sri Tumpuk
Keyword(s):  
Screening Test ◽  
Venous Blood ◽  
The Body ◽  
Ethylene Diamine ◽  
P Value ◽  
Cross Sectional ◽  
Significant Difference ◽  
Ethylene Diamine Tetra Acetate ◽  
The Difference ◽  
Index Value

Abstract: Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk  K2EDTA dan  K3EDTA. K3EDTA  biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.

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