scholarly journals Synthetic Food Coloring as an Alternative to Worm Egg Staining Ascaris lumbricoides

2021 ◽  
Vol 9 (B) ◽  
pp. 1541-1543
Author(s):  
Steven Soenjono ◽  
Budi Setiawan ◽  
Rahmah Siti Wulandari ◽  
Suyana Suyana ◽  
Marta Atik Martiningsih

Examination of worm eggs is a way of diagnosing helminthiasis by giving 2% eosin as a dye, but this reagent is carcinogenic so an alternative is needed to replace it. Synthetic food coloring (carmoisin CI 14720) can staining worm eggs against a more contrasting background than eosin. The purpose of this study was to determine the synthetic food coloring with a concentration of 1%,2%,3%,4%,5% which can be used as an alternative to 2% eosin in the examination of Ascaris lumbricoides eggs. This type of research is observational with a cross sectional research design. Positive stool samples for Ascaris lumbricoides eggs were obtained from the Parasitology Laboratory of the University of Indonesia. Data were analyzed by ANOVA and Interclass Correlation Coeficien (ICC) tests. The results of the Anova test showed that there was no difference between 2% eosin and food coloring. The results of the ICC test obtained a value of 0.845, so the dye can be used as an alternative to 2% eosin in the examination of Ascaris lumbricoides eggs.

2019 ◽  
Vol 27 (2) ◽  
pp. 348-359 ◽  
Author(s):  
Charleen McNeill ◽  
Danita Alfred ◽  
Tracy Nash ◽  
Jenifer Chilton ◽  
Melvin S Swanson

Background: Nurses must balance their perceived duty to care against their perceived risk of harm to determine their willingness to report during disaster events, potentially creating an ethical dilemma and impacting patient care. Research aim: The purpose of this study was to investigate nurses’ perceived duty to care and whether there were differences in willingness to respond during disaster events based on perceived levels of duty to care. Research design: A cross-sectional survey research design was used in this study. Participants and research context: Using a convenience sample with a snowball technique, data were collected from 289 nurses throughout the United States in 2017. Participants were recruited through host university websites, Facebook, and an American Nurses Association discussion board. Ethical considerations: Institutional review board approval was obtained from the University of Texas at Tyler and the University of Arkansas. Findings: Analysis of willingness to report to work based on levels of perceived duty to care resulted in the emergence of two groups: “lower level of perceived duty to care group” and “higher level of perceived duty to care group.” The most discriminating characteristics differentiating the groups included fear of abandonment by co-workers, reporting because it is morally the right thing to, and because of imperatives within the Nursing Code of Ethics. Discussion: The number of nurses in the lower level of perceived duty to care group causes concern. It is important for nursing management to develop strategies to advance nurses’ safety, minimize nurses’ risk, and promote nurses’ knowledge to confidently work during disaster situations. Conclusion: Level of perceived duty to care affects nurses’ willingness to report to work during disasters. Primary indicators of low perceived duty to care are amenable to actionable strategies, potentially increasing nurses’ perceived duty to provide care and willingness to report to work during disasters.


2009 ◽  
Vol 138 (7) ◽  
pp. 1004-1011 ◽  
Author(s):  
D. SCHÖNBERG-NORIO ◽  
L. MATTILA ◽  
A. LAUHIO ◽  
M.-L. KATILA ◽  
S.-S. KAUKORANTA ◽  
...  

SUMMARYThis study aimed to investigate the occurrence of complications, especially musculoskeletal symptoms, after sporadic Campylobacter jejuni enteritis of domestic origin in Finland. This multi-centre cross-sectional study was conducted during a seasonal peak in 2002. Questionnaires were sent to Campylobacter-positive patients, representing different geographical areas, 2 months after collection of positive stool samples. Medical records were viewed in several cases. Besides antimicrobial susceptibility testing C. jejuni isolates were serotyped. A total of 235 patients (58%) returned the questionnaire and 201 C. jejuni-positive patients were finally included in the study. Musculoskeletal symptoms associated with C. jejuni enteritis were frequent (39%); joint pain was most commonly reported (81%). The incidence of reactive arthritis was 4% and that of Achilles enthesopathy and/or heel pain was 9%. Stomach ache during enteritis was associated with the later development of joint pain. Antimicrobial treatment was common but did not prevent complications.


2021 ◽  
Vol 7 (2) ◽  
pp. 148
Author(s):  
Lina Fiitriani ◽  
Hilki Ofan

The problem of stunting (stunting) is one of the nutritional problems faced in the world, especially in poor and developing countries. This study aims to determine the relationship between age and parity with the incidence of stunting in children aged 0-59 months. The research design used was analytical survey method with a cross sectional research approach. The population in this study were all children aged 0-59 months. The number of samples was 67 children. The results showed that there was a relationship between age and the incidence of stunting in children aged 0-59 months with a value of 0.000 (<0.05). While the results ofthe research on the relationship between parity and the incidence of stunting in children aged 0-59 months, the value was 0.002 (<0.05), which means that there is a relationship between parity and the incidence of stunting in children aged 0-59 months.


2016 ◽  
Vol 10 (2) ◽  
pp. 68-82
Author(s):  
Kutosi Ayub Masaba ◽  
Johnson Sekakubo ◽  
Alexander Blaszczynski

Sports betting activities, particularly among youth, is increasing rapidly in Ugandan. The aim of this study was to determine the motivation for sports betting among Ugandan youth, and the social economic impact of this form of gambling. In a cross sectional quantitative research design, a questionnaire data was obtained from a sample of 181 youth attending betting houses in the Mbale district.  Results indicated that that most youths were motivated by the desire to win money. Findings also revealed that Ugandan youth socially and economically benefited from sports betting. The study concludes by recommending that even if findings do not show the negative impacts of gambling on youth, there is need for stricter laws to avert any future negative implications.


Author(s):  
Ivy Tam ◽  
Courtney McNamara ◽  
Julie Dunbar ◽  
Katherine O’Connor ◽  
Gonzalo Manzano ◽  
...  

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a respiratory virus that can cause gastrointestinal (GI) symptoms, with studies demonstrating detection of stool viral RNA weeks after respiratory tract clearance. It is unknown if children who test negative for SARS-CoV-2 on a nasopharyngeal (NP) swab may be shedding the virus in their stool. OBJECTIVE: To measure the prevalence of SARS-CoV-2 stool shedding in children with positive and negative SARS-CoV-2 NP polymerase chain reactions (PCR) tests, and to determine clinical factors associated with GI shedding. METHODS: In this cross-sectional study, we enrolled hospitalized patients 0 to 21 years old with a positive or a negative SARS-CoV-2 NP PCR test who had respiratory and/or GI symptoms. Participants were surveyed, and stool samples were sent for viral PCR testing. Fisher’s exact test was used to evaluate bivariate associations of stool PCR test positivity with categorical variables. RESULTS: Sixty-seven patients were consented; 34 patients did not provide stool samples so 33 patients were included: 17 NP-positive and 16 NP-negative for SARS-CoV-2. Eight of the 17 NP-positive patients had a positive stool PCR test for SARS-CoV-2, while none of the 16 SARS-CoV-2 NP-negative patients had a positive result (P &lt; .01). For the 17 SARS-CoV-2 NP-positive patients, GI symptoms were associated with a positive stool PCR test (P = .05) for SARS-CoV-2, but this association was not found for all 33 patients (P = .11). No associations were found with patients in an immunocompromised state or those with a comorbid condition, fever and/or chills, respiratory symptoms, headache and/or myalgias, or anosmia and/or ageusia. CONCLUSIONS: SARS-CoV-2 GI shedding is common and associated with GI symptoms in NP-positive children, with 47% having positive stool PCRs for SARS-CoV-2. GI shedding was not demonstrated in SARS-CoV-2 NP-negative children.


2018 ◽  
Vol 15 (2) ◽  
pp. 8-23
Author(s):  
Luz Marina Bautista Rodríguez ◽  
Yari Paola García Calderón ◽  
Lessly Johana Medina Chaves ◽  
Karley Ginary Cruz Hernández

Objective: To determine the perception the family has about the humane care in the Intensive Care Units of three Health Service Institutions in the city of Cucuta (Colombia). Materials and Methods: A quantitative cross-sectional descriptive study was performed with a sample of 220 family members that met the requirements of inclusion; for the recollection of the information, the instrument used was denominated Family perception about humanization in the intensive care units, designed and validated in Cucuta by students and teachers of VIII semester from the Nursing Program at the University Francisco de Paula Santander in 2016. To determine the liability the Cronbach’s Alpha was used, obtaining a value of 0.89. Results: The humane care perception by family members was moderate in 63% of the participants. The communication and information, emotional support and family participation were the highest rating categories, while coping and visits had the lowest ratings. Conclusions: The participants of the study perceive as moderate the humane care, since they relate this to the quality of the care provided, considering the evaluated categories in the research. On the other hand, the family members point out that they receive the necessary information and support to involve themselves in the care activities, allowing them for more interaction with the hospitalized familiar and the personnel.


Author(s):  
Traoré Roukiatou Zongo Cheikna ◽  
Zongo Oumarou Ouédraogo Ganamé Abasse ◽  
Zida Adama Yonaba Caroline ◽  
Traoré Yves Savadogo Aly

In sub-Saharan Africa, Gastrointestinal parasites infections are widespread, particularly among immune deficient individuals. Prevalence of these infections varies from locality to other due to many factors. The present cross-sectional study aimed to assess the prevalence of intestinal parasites among HIV-positive children in the paediatric clinic at the University Hospital Yalgado Ouedraogo of Ouagadougou. A cross-sectional study was conducted in the University hospital of Ouagadougou from July to November 2015. Ninety-three (93) freshly issued stool samples were collected in HIV-positive children less than 15 years old. Stool samples were analysed for the presence of various intestinal parasites using direct microscopy, formol-ether concentration method, modified Ziehl Neelsen stain and Weber trichrome stain. The study included 93 HIV positive children. The prevalence of intestinal parasitic infection was 24.73% (23/93). The most detected parasites were Entamoeba coli (52.17% of total detected parasites), Trichomonas intestinalis (21.73%), Giardia intestinalis (8.65%), Entamoeba histolytica (4.34%). Patients with multi-infection (2 or 3 parasites) have been detected. The participants, 97.5% (89/93) were on cotrimoxazole prophylaxis and 81.76% (76/93) were on ARV therapy. Despite the medical care given to the HIV-infected children, the prevalence of opportunistic intestinal is high at the University hospital Yalgado Ouédraogo (CHU-YO)and deserves special attention.


2020 ◽  
pp. 1-2
Author(s):  
Shir Mohammad Amirzda ◽  

Introduction: Strabismus is a clinical condition in which the eyes are not aligned properly and are pointed in different directions when looking at an object. Strabismus is a type of illness that requires medical and surgical treatment. If left untreated, amblyopia will increase the eyes of patients. Objective: Prevalence of strabismus in children visiting at University Eye Hospital in March 25, and 21 September 2019. Method and Materiel: The research design was cross-sectional study.The ophthalmology research site was university eye hospital. The total included cases were 1862 Strabismus patients the age was sixteen and under who were referred for examination and treatment of ocular diseases within the prescribed period. Age and sex information were collected from patients’ files were analyzed using Microsoft excel. Patients by age were into four groups (0–1) years, (2–5) years, (6–10) years and (11–16) years. Result: This study were examined the numbers of (1862) children in the 25 March to 21 September 2019 at the university eye hospital. Including their number of strabismus patients during the study was 3.59%. The average age is 9 years. The number of boys 37 (55.22%) and the number of girls is 30 (44.77%). The most common type of strabismus is the esotropia form (73.1%), exotropia were (26.9%). Alternate strabismus (55.2%), unilateral strabismus (44.8%). Conclusion: This study shows that the most common type of strabismus was esotropia boys were more affected than girls.


Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 519
Author(s):  
Kristýna Vaverková ◽  
Martin Kracík ◽  
Lenka Ryšková ◽  
Pavla Paterová ◽  
Rudolf Kukla ◽  
...  

Clostridioides difficile is the most common pathogen responsible for hospital-acquired diarrhea. This complication of antibiotic treatment mainly endangers the health of elder patients. Preventing the development of C. difficile infections (CDI) is still a challenge that needs to be addressed. In our study, the results of 872 C. difficile positive stool samples were used to describe the epidemiological situation affected by a change in the prescription of fluoroquinolone antibiotics. In a total, 93 of strains were typed by polymerase chain reaction (PCR) and capillary gel electrophoresis. Between years 2014 and 2018 the decline in the fluoroquinolones consumption was 69.3 defined daily dose (DDD) per 1000 patient-days (from 103.3 to 34.0), in same period CDI incidence declined by 1.3 cases per 10,000 patient-bed days (from 5.6 to 4.3). Results of epidemiologic and statistical analysis shows that decline in fluoroquinolones consumption has significant influence on CDI incidence and prevalence of hypervirulent strains. In the University Hospital Hradec Králové properly managed antibiotic stewardship policy has reduced CDI incidence by 23.2% and lowered rate of hypervirulent ribotypes 001 and 176.


Author(s):  
Aqsa Noor ◽  
Muhammad Azam Khan ◽  
Bareera Saeed ◽  
Hafsa Nareen ◽  
Fahad Massod

Aims: To determine the awareness of stammering disorder among university students of related fields that are Speech Language Pathology, Psychology, and Allied Professionals. Study design: Cross sectional survey is used as research design in this study. Place and Duration of Study: University of Central Punjab, The University of Lahore. Superior University, Lahore campus between June 2020 and July 2021. Methodology: Sample (N=333) was drawn through purposive sampling technique from various universities of Lahore with age range of 17 – 35 years of 44% male and 56% female students of related fields( Speech Language Pathology, Psychology, Allied Professionals).Standardized questionnaire was used to determine the awareness. Data was analyzed through SPSS with descriptive statistic of frequency and percentages. Results: Majority of participants 59.6% had accurate awareness regarding stuttering is more common in male and stuttering is not hereditary. Conclusion: It was concluded that majority university students of related fields have accurate awareness regarding stammering disorder in Lahore, Pakistan.


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