The effects of magnetic separation on cryopreserved bovine spermatozoa motility, viability and cryo-capacitation status

Zygote ◽  
2012 ◽  
Vol 22 (3) ◽  
pp. 378-386 ◽  
Author(s):  
S.S.M. Faezah ◽  
F.M.Y Zuraina ◽  
J.H.F. Farah ◽  
O. Khairul ◽  
N.I. Hilwani ◽  
...  
Keyword(s):  
Annexin V ◽  
Control Group ◽  
Preparation Technique ◽  
Sperm Preparation ◽  
Spermatozoa Motility ◽  
Male Gametes ◽  
Significant Difference ◽  
Long Time ◽  
Bovine Spermatozoa ◽  
Magnetic Activated Cell Sorting

SummaryCryopreservation is a technique used to preserve cells for long-time storage. It is widely used in agriculture to store male gametes in liquid nitrogen. The aim of this study was to determine the optimum thawing temperature and time for samples subjected to annexin V magnetic-activated cell sorting (AnMACS) as the sperm preparation technique. Pooled semen samples from three ejaculates were divided into two groups. The treatment group was subjected both to AnMACS and to being cryopreserved, whilst the control group was cryopreserved directly without MACS. Post-thaw analysis was carried out for samples thawed at either 20°C for 13 s, 37°C for 30 s, 40°C for 7 s, 60°C for 6 s or 80°C for 5 s. Sperm kinematics, viability and capacitation status were determined for samples subjected to all thawing temperatures described. Results showed that thawing at 37°C for 13 s for MACS-processed samples was a superior option compared with other thawing procedures; there was a significant difference in P < 0.05 values for curvilinear velocity (VCL μm/s) and sperm straightness (STR %) when samples were thawed at 40°C for 7 s, with fewer capacitated spermatozoa (P < 0.05) when samples were thawed at 37°C for 30 s, 40°C for 7 s or 60°C for 6 s. Hence, we can speculate that the use of AnMACS as the sperm preparation technique can somehow enhance sperm cryosurvival rate after cryopreservation, however the fertilization potential of these cells has yet to be determined.

8 THE USE OF ANNEXIN V MAGNETIC-ACTIVATED CELL SORTING TO SEPARATE APOPTOTIC SPERM FROM THE EJACULATE OF STALLIONS

2011 ◽  
Vol 23 (1) ◽  
pp. 110
Author(s):  
M. A. Coutinho da Silva ◽  
C. R. F. Pinto ◽  
J. M. Young ◽  
K. Cole
Keyword(s):  
Cell Sorting ◽  
Semen Quality ◽  
Sperm Quality ◽  
Cleveland Clinic ◽  
Membrane Integrity ◽  
Annexin V ◽  
Sperm Parameters ◽  
Control Group ◽  
Frozen Semen ◽  
Magnetic Activated Cell Sorting

Magnetic-activated cell sorting (MACS) has been used successfully in humans to remove apoptotic sperm from the ejaculate. Annexin V-conjugated microbeads recognise sperm with externalized phosphatidylserine, which is considered one of the features of apoptosis, and the labelled sperm is separated by MACS. The goals of the study were to determine if MACS can be used to separate apoptotic sperm from the ejaculate of stallions; and to determine if removal of apoptotic sperm improves the quality of stallion sperm. Our hypothesis was that MACS would improve semen quality by removing apoptotic sperm, resulting in samples with higher motility and viability. Two ejaculates from three different stallions of good fertility were used. Sperm were diluted with Tyrode’s albumin lactate pyruvate (TALP) and incubated with annexin V-conjugated microbeads for 15 min at 37°C. Control samples were incubated in the absence of annexin V microbeads. The suspension was then loaded into the separation column containing iron globes, which were fitted in a magnet (MiniMACS; Miltenyi Biotec Inc., Auburn, CA, USA). The effluent sample containing annexin-negative sperm was collected and then, the column was removed from the magnetic field and rinsed with TALP to collect the annexin-positive cells. Sperm viability, motility, morphology and caspase activation were determined in all three samples: control, annexin-negative, and annexin-positive. Data were evaluated by ANOVA and individual comparisons were performed by Tukey’s hsd test. Significance was set at P < 0.05 and data is presented as means ± SEM (Table 1). The main effect of stallion was significant only for sperm motility parameters. Sperm recovery rate following MACS was 46 ± 3%. In conclusion, the use of MACS was effective in removing apoptotic sperm from the ejaculate. The annexin-positive population displayed a higher proportion of sperm with activated caspases and lower membrane integrity and motility. However, removal of apoptotic sperm from the ejaculate did not improve sperm parameters in the annexin-negative group compared to control group. In addition, sperm morphology was not affected by MACS. Further studies are necessary to determine if MACS could be used successfully to improve sperm quality from subfertile stallions and frozen semen. Table 1.Sperm parameters following annexin V MACS (mean ± SEM) The authors are thankful to Mark Williams at Miltenyi Biotec Inc. for providing supplies; and Dr Ashok Agarwal at The Center for Reproductive Medicine, Cleveland Clinic, for scientific input.

302 VIABILITY OF BOVINE SPERMATOZOA INCUBATED WITH FOREIGN DNA

2006 ◽  
Vol 18 (2) ◽  
pp. 258
Author(s):  
W. B. Feitosa ◽  
M. P. Milazzoto ◽  
M. Rovegno ◽  
L. F. Martins ◽  
J. A. Visintin ◽  
...  
Keyword(s):  
Incubation Period ◽  
Molecular Probes ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Sperm Viability ◽  
Exogenous Dna ◽  
Significant Difference ◽  
Class 1 ◽  
Bovine Spermatozoa ◽  
Over Time

Several studies have been performed over the years to promote the understanding and improvement of the sperm-mediated gene transfer technique. However, little is known about the effect of exogenous DNA in the sperm cells. The aim of this study was to evaluate the effect of the incubation period and exogenous DNA addition on mitochondrial activity and acrosomal status of bovine spermatozoa. Frozen-thawed semen was separated by Percoll gradient (45/90%) at 600g for 30 min, and the pellet was resuspended and washed by centrifugation in sperm-TALP at 200g for 5 min. The spermatozoa were resuspended in fertilization medium (without heparin) at a concentration of 5 × 106 spermatozoa/mL and incubated at 39°C, 5% CO2 in air with 500 ng pEYFP-Nuc/mL (Clontech, Mountain View, CA, USA) (DNA group) for 1 and 2 h or without DNA (control group) for 0, 1 and 2 h. JC-1 (Molecular Probes; Invitrogen Brasil, Ltd., Sao Paulo, Brazil) was used to determine mitochondrial potential and fluorsecein isothiogyanate (FITC-PSA; Sigma-Aldrich, Brazil) was used to assess acrosomal status. Two microliters of JC-1 (76.5 μM in DMSO) and 50 μL of FITC-PSA (100 μg/mL in DPBS) were added to 150 μL of IVF medium + 5 × 106 spermatozoa/mL; the mixture was incubated at 25°C for 10 min. DNA incorporation was evaluated by p-EYFP-Nuc PCR amplification. All treatments were repeated ten times and data were analyzed by ANOVA and Tukey test (P < 0.05). The results are described in Table 1. The sperm were classified as: Class 1 (intact acrosome and high mitochondria potential), Class 2 (intact acrosome and low mitochondria potential), Class 3 (reacted acrosome and high mitochondria potential) and Class 4 (reacted acrosome and low mitochondria potential). In both groups, the Class 1 sperm decreased over time, whereas an increase in number was verified for Class 2 sperm. There was no significant difference in numbers among the incubation periods in both groups for Class 3 sperm. However, there was an increase in Class 4 sperm number over time, indicating that the main effect of the incubation period was the loss of mitochondria potential. There was no effect of the exogenous DNA addition on sperm viability in relation to the control group, indicating that the exogenous DNA had no effect on mitochondrial activity and acrosomal status of bovine semen. Table 1. Effect of incubation period and exogenous DNA addition on sperm viability This work was supported by FAPESP 03/10234–7; 03/07456–8.

RNA Interference Against Bcl-2 mRNA Increases Radiosensitivity of Raji Cells.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4601-4601
Author(s):  
Dongmei He ◽  
Yuan Zhang ◽  
Gexiu Liu
Keyword(s):  
Caspase 3 ◽  
Radiation Treatment ◽  
Annexin V ◽  
Treated Group ◽  
Parp Cleavage ◽  
Control Group ◽  
Raji Cells ◽  
Protein Levels ◽  
Significant Difference ◽  
Induced Apoptosis

Abstract Bcl-2 is the prominent member of a family of proteins responsible for dysregulation of apoptosis, and resistance to chemotherapy. It has been shown that reduction in Bcl-2 protein levels could ultimately induce a lower apoptotic threshold and restore chemosensitivity in a variety of malignancies. Short interfering RNA (siRNA) has been evaluated as an attractive and effective tool for suppressing a target protein by specifically digesting its mRNA. In our lab, we have identified a siRNA targeting against Bcl-2 could effectively down-regulate Bcl-2 protein. In this study, we investigated the effect of gamma radiation combined with the siRNA targeting Bcl-2 on proliferation and apoptosis in B-lymphoma Raji cells. The siRNA was introduced into cells using Oligofectamine transfection. Cells were treated with Bcl-2 siRNA alone or with 2–8 Gy dose of (60)Co gamma rays. Expression of Bcl-2 mRNA and protein was assayed by quantitative reverse transcriptase-polymerase chain reaction and Western blotting analysis. Radiosensitivity was determined by clonogenic cell survival assay. Apoptosis was determined by Giemsa staining, Annexin-V binding assay and flow cytomertry. Furthermore caspase-3 activity and poly (ADP-ribose) polymerase (PARP) cleavage were evaluated. Transfection of Raji cells with 100 nmol/L siRNA targeted against Bcl-2 resulted in reduction of Bcl-2 mRNA by 75% compared with control-siRNA treated group and the vehicle control group(p<0.05). The levels of Bcl-2 protein were significantly reduced by 70% compared with the two control groups (p<0.05). There was significant difference in the radiosensitivity of Raji cells in which Bcl-2 was silenced compared with the cells transfected vehicle or control siRNA.Apoptosis index of the Raji cells treated with Bcl-2 siRNA combined with radiation was significantly increased (p<0.05), compared with either control siRNA / radiation combination or radiation-treatment cells alone, or Bcl-2 siRNA-treatment cells alone.Raji cells treated with Bcl-2 siRNA combined with radiation revealed enhanced caspase-3 and PARP cleavage as compared to Bcl-2 siRNA treated cells alone or only irradiated cells. These findings show that Bcl-2 siRNA synergistically enhances radiation-induced apoptosis through the expression of proteins involved in the programmed cell death.

scholarly journals Pengaruh pemberian jus jeruk manis (citrus sinensis.) terhadap nilai VO2 max atlet sepak bola di Gendut Dony Training Camp (GDTC) Salatiga

2017 ◽  
Vol 5 (2) ◽  
pp. 68-74
Author(s):  
Sofia Arum Andani ◽  
Nurmasari Widyastuti
Keyword(s):  
Orange Juice ◽  
The Body ◽  
Control Group ◽  
Training Camp ◽  
Glucose Levels ◽  
Total Food Intake ◽  
Subject Characteristic ◽  
Significant Difference ◽  
Post Test ◽  
Long Time

Background : Endurance is the ability of  the body to perform activities or work for a long time without experiencing fatigue. Based on using of energy systems, endurance is divided into aerobic and anaerobic. Aerobic endurance is measured using VO2max value. Aerobic endurance is supported by the availability of carbohydrates and fats. Intake of carbohydrates as much as 30-60 grams/hour can maintain glucose levels and maintain the level of burning carbohydrates in the body, so it can improve the endurance about 30-60 minutes. Carbohydrate content in 300 ml of sweet orange juice is 54,9 grams that can be used to increase endurance atheletes. Objective : The purpose of this study was to analyze the effect of orange juice on VO2max value  in football athletes. Method : This study was in the field of  experimental design with post test only with controlled group design.  Subject for these study were tweenty-one football athletes which are match with inclusion criteria in Gendut Dony Training Camp (GDTC) Salatiga. The Subject has given 300 ml orange juice and 300 ml placebo 30 minutes before test. VO2max value was measured by using Balke running test. All datas were analyzed by Independent Sample T-tes. Result : Subject characteristic includes age, weight, height, body mass index (BMI), and total food intake were not showing significant difference (p<0,05), therefore subject were categorized as homogen. Significant difference was showed in average of  VO2max value between the treatment group (43,67±2,26) and control group (39,33±4,39). Conclusion : Consumption of orange juice 30 minutes before exercising shows a significant impact to increase the VO2max value.

scholarly journals 121 HEAT SHOCK TO PIG OOCYTES DOES NOT INDUCE APOPTOSIS BUT REDUCES EMBRYO DEVELOPMENT

2005 ◽  
Vol 17 (2) ◽  
pp. 211
Author(s):  
J.-K. Tseng ◽  
P.-C. Tang ◽  
J.-C. Ju
Keyword(s):  
Heat Shock ◽  
Apoptotic Cell ◽  
Electric Pulse ◽  
Annexin V ◽  
Developmental Competence ◽  
Control Group ◽  
Hsp 70 ◽  
Cell Numbers ◽  
Significant Difference

Oocytes are susceptible to heat shock (HS) during the maturation process. It has been demonstrated that HS induces apoptosis and/or the expression of HS protein 70 (hsp 70) in in vitro-produced oocytes and embryos. The objectives of this study were to analyze the effects of HS on the development and apoptosis of pig oocytes and embryos. Porcine ovaries were collected from a local slaughterhouse and the cumulus-oocyte complexes (COCs) were aspirated from follicles 3–6 mm in diameter and subjected to standard in vitro maturation procedures at 39°C for 42 h. The in vitro matured oocytes were then randomly allocated to different HS treatments at 41.5°C for 0 (control, C0h), 1 (HS1h), 2 (HS2h), or 4 h (HS4h). An additional control group of oocytes was cultured for 4 h without HS (C4h). Data were analyzed by chi-square test. In Experiment 1, anti-hsp 70 (SPA-810AP, Stressgen, San Diego, CA, USA) and Western blotting were used to examine the expression of hsp 70. Results indicated that no significant difference of hsp 70 expression in metaphase II porcine oocytes occurred between controls and HS groups (P > 0.05, 7 replicates). In Experiment 2, apoptosis of metaphase II oocytes after HS was identified by annexin V-FITC (Sigma, St. Louis, MO, USA) staining and TUNEL (Roche, Indianapolis, IN, USA). No significant apoptotic signal was detected in the HS groups compared to the controls. The intensity of annexin V staining was not affected by HS, but it increased with the time of culture (P < 0.05, n = 24–37). In Experiment 3, the apoptotic rate and developmental competence of the HS-oocytes were evaluated by TUNEL assay (n = 123–137, 4 replicates). Parthenogenetic activation (n = 123–137) was performed by an electric pulse (2.2 kV cm−1) combined with 6-dimethyaminopurine treatment (6-DMAP, 2.5 μM, 4 h, Sigma). The cleavage rates in HS2h (43 ± 29%) and HS4h (35 ± 28%) decreased (P < 0.05) compared to those in C0h (62 ± 12%) and C4h (66 ± 8%). In addition, the blastocyst formation rates and total cell numbers reduced (P < 0.05) after 2 h (11 ± 10%, 20 ± 16) and 4 h (11 ± 8%, 19 ± 8) of HS treatments compared to those in C0h (23 ± 14%, 32 ± 22) and C4h (21 ± 11%, 27 ± 17), all respectively. The numbers of blastocysts with TUNEL-positive signals were not significantly different between the HS and control groups, but the signals increased (P < 0.05) before the 8-cell stage in HS groups (22–24%) compared to the C0h and C4h controls (16 and 11%), respectively. These results indicate that reduction in developmental competence of in vitro-matured pig oocytes after heat shock is not closely correlated to the expression of hsp 70 in the oocytes and to the apoptotic cell numbers in the blastocyst. Whether detection of apoptosis by TUNEL or annexin V-FITC in oocytes is a good indicator requires further investigation.

scholarly journals Influence of matrix nature on the functional efficacy of biomedical cell product for the regeneration of damaged liver (experimental model of acute liver failure)

2017 ◽  
Vol 19 (2) ◽  
pp. 78-89
Author(s):  
S. V. Gautier ◽  
M. Yu. Shagidulin ◽  
N. A. Onishchenko ◽  
I. M. Iljinsky ◽  
V. I. Sevastianov
Keyword(s):  
Liver Failure ◽  
Acute Liver Failure ◽  
Experimental Model ◽  
Cell Activity ◽  
Liver Cells ◽  
Control Group ◽  
Nanostructured Composite ◽  
Hydrogel Matrix ◽  
Significant Difference ◽  
Long Time

Aim. A comparative analysis of the functional efficacy of biomedical cell products (BMCP) for the regeneration of damaged liver based on biopolymer scaffolded porous and hydrogel matrices was performed on the experimental model of acute liver failure. Materials and methods. Matrices allowed for clinical use were employed for BMCP in the form of a sponge made from biopolymer nanostructured composite material (BNCM) based on a highly purified bacterial copolymers of poly (β-hydroxybutyrate-co-β-oxyvalerate) and polyethylene glycol and a hydrogel matrix from biopolymer microheterogeneous collagen-containing hydrogel (BMCH). Cellular component of BMCP was represented by liver cells and multipotent mesenchymal bone marrow stem cells. The functional efficacy of BMCP for the regeneration of damaged liver was evaluated on the experimental model of acute liver failure in Wistar rats (n = 40) via biochemical, morphological, and immunohistochemical methods. Results. When BMCP was implanted to regenerate the damaged liver on the basis of the scaffolded BNCM or hydrogel BMCH matrices, the lethality in rats with acute liver failure was absent; while in control it was 66.6%. Restoration of the activity of cytolytic enzyme levels and protein-synthetic liver function began on day 9 after modeling acute liver failure, in contrast to the control group, where recovery occurred only by days 18–21. Both matrices maintained the viability and functional activity of liver cells up to 90 days with the formation of blood vessels in BMCP. The obtained data confirm that scaffolded BNCM matrix and hydrogel BMCH matrix retain for a long time (up to 90 days) the vital activity of the adherent cells in the BMCP composition, which allows using them to correct acute liver failure. At the same time, hydrogel matrix due to the presence of bioactive components contributes to the creation of the best conditions for adhesion and cell activity which accelerate the regeneration processes in the damaged liver compared to BMCP on scaffolded matrix. Conclusion. A statistically significant difference was found between the functional efficacy of the BMCP studied based on BNCM and BMCH matrices. BMCP based on hydrogel BMCH matrix was more effective for the regeneration of damaged liver.

Effect of the MACS technique on rabbit sperm motility

2011 ◽  
Vol 6 (6) ◽  
pp. 958-962
Author(s):  
Jaromir Vasicek ◽  
Alexander Makarevich ◽  
Peter Chrenek
Keyword(s):  
Harmful Effect ◽  
Sperm Concentration ◽  
Annexin V ◽  
Outer Leaflet ◽  
Significant Difference ◽  
Sperm Plasma Membrane ◽  
And Control ◽  
Casa System ◽  
Control Samples ◽  
Magnetic Activated Cell Sorting

AbstractMagnetic-activated cell sorting (MACS) separates apoptotic spermatozoa by the use of annexin V-conjugated nanoparticles which bind to phosphatidylserine that is externalized on the outer leaflet of the sperm plasma membrane. This technique yields two fractions: annexin V-negative (AnV−) and annexin V-positive (AnV+). The aim of the study was to evaluate the effect of MACS application on the motility parameters of rabbit spermatozoa. Rabbit semen samples collected separately from 4 bucks (I, II, III, and IV) were filtered and separated in a MACS system. The semen samples from a control (untreated) group, AnV− and AnV+ fraction were evaluated using CASA system. The experiment was replicated 4 times for each buck. The AnV+ sperm had significantly lower concentration than the AnV− fractions and the control samples (P<0.05 for bucks I, II, III, but not IV). We observed that the proportion of apoptotic spermatozoa in the semen of NZW bucks is about 20%. There was no significant difference in the percentage of motile and progressively motile spermatozoa between the AnVfractions and control samples. In conclusion, the MACS technique has no harmful effect on the rabbit sperm concentration and motility.

scholarly journals The Using Piper ornatum as Biopesticide Larvae Musca domestica

BIOEDUSCIENCE ◽  
2020 ◽  
Vol 4 (1) ◽  
pp. 106-112
Author(s):  
Anita Dewi Moelyaningrum ◽  
Violita Pita Nugraheni ◽  
Prehatin Trirahayu Ningrum
Keyword(s):  
Treatment Group ◽  
Essential Oils ◽  
Experimental Method ◽  
Musca Domestica ◽  
House Fly ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Long Time

Background: Red betel (Piper ornatum) contains several compounds including flavonoids, alkaloids, tannins, and essential oils that have the ability as bioinsecticides. The purpose of this study was to analyze the differences in the number of deaths of house fly larvae (Musca domestica) exposed to red betel in the control group (0%) and the treatment group with a concentration of 1%; 1.5% and 2% for 24 hours. Methods: used is true experimental method with only posttes control group design. There were 4 treatments with 6 replications per treatment. Each treatment was described on 8 larvae, so the number of larvae used in this study was 192 tails. Results: Research shows that red betel powder is indeed effective in killing Musca domestica larvae, but it still requires a long time, which is at least 24 hours. Concentration is needed at least 2% if used as a biopesticide to reduce the density of flies. Conclusion: There was no significant difference in the mortality of Musca domestica larvae in the administration of red betel powder (Piper ornatum.  

scholarly journals Mechanical properties and microstructural analysis of an AgPd alloy cast under different temperatures

2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Karina Andrea Novaes Olivieri ◽  
Maximiliano Piero Neisser ◽  
Marco Antônio Bottino ◽  
Milton Edson Miranda ◽  
Rafael Dario Wernek
Keyword(s):  
Mechanical Properties ◽  
Elevated Temperatures ◽  
Microstructural Analysis ◽  
Casting Temperature ◽  
Control Group ◽  
Significant Difference ◽  
Different Temperatures ◽  
Long Time ◽  
New Material ◽  
T1 And T2

The metal restorations are used in Dentistry a long time ago. Nowadays we have resources that can get casting more accurate, with new material and equipments and techniques more precise. The purpose of this study was to evaluate the metallurgical and mechanical aspects of the AgPd dental alloy when it was submitted to different casting temperatures. It was used 30 specimens, divided in three groups (n=10): a) control group (no cast); b) casting temperature in accordance with the manufactures’ instructions (T1); b) casting temperature above manufactures´ instructions (like a torch) (T2). It was evaluated chemical and metallographic aspects, mechanical properties and Vickers hardness. The results showed a microstructure similar to T1 and T2 conditions, but with greater amount of light phase and particles in this last one. It was observed that the rupture tensile strength for the T1 condition was greater than the T2. The alloy in the no cast condition presented greater hardness but there was no statistically significant difference between T1 and T2. Supporting by the metallographic and mechanical results, it may predict that when elevated temperatures was used, above manufacture´s recommendations, it can occur failures in the prosthesis, like porosities, fissures or cracks.

scholarly journals EFFECT OF VITAMIN E (α-TOCOPHEROL) ADMINISTRATION ON SPERM MOTILITY AND MORPHOLOGY OF SPRAGUE DAWLEY STRAIN RATS AFTER CISPLATIN TREATMENT

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Dimas Visa Aditya ◽  
Tarmono Djojodimedjo ◽  
Doddy M Soebadi
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
Control Group ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Control Groups ◽  
Spermatozoa Motility ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Tocopherol Isomer

Objective: To evaluate the protective effects of vitamin E α-tocopherol isomer against the toxicity of cisplatin on sperm motility and morphology in Sprague Dawley rats. Material & Methods: Twenty-four rats were grouped into four groups (n=6). The control group (CN) was injected with normal saline, second group (CP) was injected with cisplatin, the third group (P1) was injected with cisplatin and vitamin E 50 mg/kgBW for 7 weeks P.O, the fourth group (P2) was injected with cisplatin and vitamin E 200 mg/kgBW for 7 weeks P.O. Vitamin E was given from 3 weeks before cisplatin injection and 4 weeks following cisplatin injection. At 7th week, all the samples were undergoing bilateral orchidectomy. Vitamin E that being used in this study was α-tocopherol isomer. Results: Cisplatin decreased motility and morphology of spermatozoa significantly against controls. Vitamin E 50 mg/kgBW and 200 mg/kgBW significantly increased motility of spermatozoa (p<0.05) compared to those in the cisplatin group only. Vitamin E 50 mg/kgBW, and 200 mg/kgBW did not have a significant difference in spermatozoa motility compare to control groups. Vitamin E 50 mg/kgBW and 200 mg/kgBW could increase the spermatozoa morphology significantly compare to those cisplatin only group. Vitamin E 50 mg/kgBW, and 200 mg/kgBW did not have a significant difference in spermatozoa morphology compared to control groups. Conclusion: α-tocopherol 50 mg/kgBW and 200 mg/kgBW provided a same protective effect against spermatozoa damage especially in motility and morphology aspect due to cisplatin exposure. Therefore, in this study it was more recommended to use α-tocopherol in 50 mg/kgBW dose than 200 mg/kgBW.

Sign in / Sign up

Export Citation Format

Share Document