Flavonoid content in seeds of guar germplasm using HPLC

2007 ◽  
Vol 5 (02) ◽  
pp. 96-99 ◽  
Author(s):  
M. L. Wang ◽  
J. B. Morris
Keyword(s):  
High Performance ◽  
Significant Positive Correlation ◽  
Seed Quality ◽  
Soybean Seeds ◽  
Cyamopsis Tetragonoloba ◽  
Flavonoid Content ◽  
Beneficial Effects ◽  
Breeding Programmes ◽  
Guar Seed ◽  
Germplasm Accessions

Legume flavonoids have received considerable attention due to their beneficial effects on human health. Flavonoid content in the seeds of 36 germplasm accessions of guar [Cyamopsis tetragonoloba(L.) Taub.] was quantified by high-performance liquid chromatography (HPLC). On a per 100 g basis, the seed contained, on average, 1.114 mg (0–2.355) daidzein, 0.700 mg (0–1.685) genistein, 0.553 mg (0–1.250) quercetin and 14.460 mg (10.70–19.82) kaempferol. A significant positive correlation was observed between the content of daidzein and genistein; however, significant negative correlations existed between the content of kaempferol and daidzein and of kaempferol and genistein. Compared to soybean seeds, guar seeds contained very low amounts of daidzein and genistein, but a high amount of kaempferol. The information about the levels of flavonoids in guar seeds will be useful to breeding programmes for improving guar seed quality. Furthermore, the high kaempferol content of guar seeds may expand its nutraceutical and pharmaceutical utilization.

Flavonoid content in different legume germplasm seeds quantified by HPLC

2008 ◽  
Vol 6 (1) ◽  
pp. 62-69 ◽  
Author(s):  
M. L. Wang ◽  
A. G. Gillaspie ◽  
J. B. Morris ◽  
R. N. Pittman ◽  
J. Davis ◽  
...  
Keyword(s):  
Seed Coat ◽  
Significant Variation ◽  
High Performance ◽  
Coat Colour ◽  
Soybean Seeds ◽  
Flavonoid Content ◽  
Seed Coat Colour ◽  
Content Variation ◽  
Legume Seeds ◽  
Breeding Programmes

Flavonoid content was quantified by high performance liquid chromatography (HPLC) and seed-coat colour was recorded from different legume seeds. Soybean seeds generally contained significantly higher amounts of daidzein (315–354 μg/g), genistein (438–458 μg/g), kaempferol (38–68 μg/g) and total measured flavonoids (892–917 μg/g), while cowpea and peanut seeds contained a significantly higher amount of quercetin (214–280 μg/g and 133–289 μg/g, respectively) than the other legumes tested. Significant variation for flavonoid content existed among and within legume species. Daidzein was significantly correlated with genistein and kaempferol (r = 0.92, P < 0.0001; r = 0.68, P < 0.0001), respectively. Genistein was also significantly correlated with kaempferol (r = 0.84, P < 0.0001). Due to differences in genetic background, no consistent relationship was observed between seed-coat colour and flavonoid content. Variation observed in flavonoid content and seed-coat colour would be useful for legume breeding programmes and consumer use.

scholarly journals Autofluorescence-spectral imaging as an innovative method for rapid, non-destructive and reliable assessing of soybean seed quality

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Clíssia Barboza da Silva ◽  
Nielsen Moreira Oliveira ◽  
Marcia Eugenia Amaral de Carvalho ◽  
André Dantas de Medeiros ◽  
Marina de Lima Nogueira ◽  
...  
Keyword(s):  
Machine Learning ◽  
High Performance ◽  
Seed Quality ◽  
Soybean Seed ◽  
Spectral Imaging ◽  
Machine Learning Algorithms ◽  
Support Vector ◽  
Soybean Seeds ◽  
Linear Discriminant ◽  
Non Destructive

AbstractIn the agricultural industry, advances in optical imaging technologies based on rapid and non-destructive approaches have contributed to increase food production for the growing population. The present study employed autofluorescence-spectral imaging and machine learning algorithms to develop distinct models for classification of soybean seeds differing in physiological quality after artificial aging. Autofluorescence signals from the 365/400 nm excitation-emission combination (that exhibited a perfect correlation with the total phenols in the embryo) were efficiently able to segregate treatments. Furthermore, it was also possible to demonstrate a strong correlation between autofluorescence-spectral data and several quality indicators, such as early germination and seed tolerance to stressful conditions. The machine learning models developed based on artificial neural network, support vector machine or linear discriminant analysis showed high performance (0.99 accuracy) for classifying seeds with different quality levels. Taken together, our study shows that the physiological potential of soybean seeds is reduced accompanied by changes in the concentration and, probably in the structure of autofluorescent compounds. In addition, altering the autofluorescent properties in seeds impact the photosynthesis apparatus in seedlings. From the practical point of view, autofluorescence-based imaging can be used to check modifications in the optical properties of soybean seed tissues and to consistently discriminate high-and low-vigor seeds.

Analysis of Brassinosteroids in Soybean Seeds and Leaves by Liquid Chromatography-Tandem Mass Spectrometry

2017 ◽  
Vol 10 (1) ◽  
pp. 100-109
Author(s):  
Hongxia Li ◽  
Natasha Trzaskalski ◽  
R.J. Neil Emery
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Soybean Seeds ◽  
Tandem Mass ◽  
Electrospray Tandem Mass Spectrometry ◽  
Aqueous Methanol ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Objective:A simple and fast high performance liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) method has been developed for the analysis of brassinosteroids (BRs) in plants without derivatization.Materials:The BRs (including castasterone, 24-epicastasterone, brassinolide and 24-epibrassinolide) have been extracted with ice cold 80% aqueous methanol solution.Method:Five different purification strategies have been tested for the purification and enrichment of BRs.Conclusion:This analytical method was sensitive, reliable, rapid and applicable to trace analysis in complex plant samples.

Identification of quantitative trait loci underlying lutein content in soybean seeds across multiple environments

2017 ◽  
Vol 155 (8) ◽  
pp. 1263-1271 ◽  
Author(s):  
W. L. TENG ◽  
W. J. FENG ◽  
J. Y. ZHANG ◽  
N. XIA ◽  
J. GUO ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Phenotypic Variation ◽  
Quantitative Trait ◽  
Average Distance ◽  
Recombinant Inbred Lines ◽  
Chromosome 9 ◽  
Soybean Seeds ◽  
Trait Loci ◽  
Breeding Programmes ◽  
Lutein Content

SUMMARYLutein benefits human health significantly, including that of the eyes, skin and heart. Therefore, increasing lutein content in soybean seeds is an important objective for breeding programmes. However, no information about soybean lutein-related quantitative trait loci (QTL) has been reported, as of 2016. The aim of the present study was to identify QTLs underlying the lutein content in soybean seeds. A population including 129 recombinant inbred lines was developed from the cross between ‘Dongnong46’ (lutein 13·10 µg/g) and ‘L-100’ (lutein 23·96 µg/g), which significantly differed in seed lutein contents. This population was grown in ten environments including Harbin in 2012, 2013, 2014 and 2015; Hulan in 2013, 2014 and 2015; and Acheng in 2013, 2014 and 2015. A total of 213 simple sequence repeat markers were used to construct the genetic linkage map, which covered approximately 3623·39 cM, with an average distance of 17·01 cM between markers. In the present study, eight QTLs associated with lutein content were found initially, which could explain 1·01–19·66% of the observed phenotypic variation in ten different tested environments. The phenotypic contribution of qLU-1 (located near BARC-Satt588 on chromosome 9 (Chr 9; linkage group (LG) K)) was >10% across seven tested environments, while qLU-2 (located near Satt192 of Chr 12 (LG H)) and qLU-3 (located near Satt353 of Chr12 (LGH)) could explain 5–10% of the observed phenotypic variation in more than seven environments, respectively. qLU-5, qLU-6, qLU-7 and qLU-8 could be detected in more than four environments. These eight QTLs were novel, and have considerable potential value for marker-assistant selection of higher lutein content in soybean lines.

scholarly journals Lignification of the plant and seed quality of RR soybeans sprayed with herbicide glyphosate

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 286-292
Author(s):  
Cristiane Fortes Gris ◽  
Edila Vilela de Resende Von Pinho ◽  
Maria Laene de Moreira Carvalho ◽  
Rafael Parreira Diniz ◽  
Thaís de Andrade
Keyword(s):  
Seed Quality ◽  
Lignin Content ◽  
Block Design ◽  
Accelerated Aging ◽  
Soybean Seeds ◽  
Randomized Block Design ◽  
Physiological Quality ◽  
Hand Weeding ◽  
Herbicide Glyphosate

Differences in levels of lignin in the plant between conventional and transgenic cultivars RR has been reported by several authors, however, there are few studies evaluating the influence of spraying of glyphosate on the lignin in the plant and RR soybean seeds. The aim of this study was to evaluate the physiological quality of RR transgenic soybean seeds and the lignin contents of plants sprayed with the herbicide glyphosate. The assays were conducted both in greenhouse and field in the municipality of Lavras, MG, in the agricultural year 2007/08. The experiment was arranged in a splitplot design with four replicates, considering the treatments hand weeding and herbicide glyphosate as plots, and five RR soybean cultivars (BRS 245 RR, BRS 247 RR, Valiosa RR, Silvânia RR and Baliza RR) as splitplots. In the greenhouse, the cultivars tested were BRS 245 RR and Valiosa RR in a randomized block design with four replicates. The sprayings were carried out at stages V3, V7 and early R5 (3L/ha). The 1000 seed weight, mechanical injury, germination and germination velocity index, emergence velocity index, accelerated aging, electrical conductivity and water soaking seed test, lignin content in the seed coat, in the stem and legumes were determined. The spraying of glyphosate herbicide, in greenhouse and field, did not alter the physiological quality of seeds and the lignin contents in the plant.

scholarly journals Antioxidant Activity and Polyphenolic Profile of Walnut (Juglans regia L.) Green Husks and Liqueurs

2020 ◽  
Vol 69 (1-2) ◽  
pp. 5-11
Author(s):  
Dijana Kulačanin ◽  
Sandra Bijelić ◽  
Jovana Šućur ◽  
Borivoje Bogdanović ◽  
Sezai Ercisli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Growth And Development ◽  
Juglans Regia ◽  
Flavonoid Content ◽  
Beneficial Effects ◽  
Cultivar Selection ◽  
Polyphenolic Content ◽  
Polyphenolic Profile ◽  
Selection Of ◽  
Time Point

SummaryThe beneficial effects of walnuts and walnut products on human health, due primarily to their rich polyphenolic content, have been appreciated as an empirical fact for centuries. The purpose of this study is to determine the polyphenolic contents of liqueurs made from the walnut selections ‘Rasna’ and ‘Sava’ and the walnut cultivar ‘Šampion’ (all harvested at three different times), as well as the polyphenolic contents of their green husks. The walnut liqueurs were prepared according to two traditional recipes using young walnut fruits. The highest antioxidant capacity was recorded in the liqueur made from the ‘Rasna’ walnut selection (89.94%), whereas the highest contents of phenols (83.28 mg GAE/g FW), flavonoids (0.83 mg QE/g FW) and proanthocyanidins were found in the liqueur made from the ‘Šampion’ cultivar (14.75 mg CE/g FW). The youngest ‘Rasna’ walnuts, harvested at the first experimental time point, exhibited the highest phenolic and tannin contents, whereas the highest flavonoid content was observed in the ‘Šampion’ cultivar. The biochemical results obtained indicate a decrease in the polyphenolic content of walnut fruits with their growth and development. Moreover, the polyphenolic profiles of the walnut liqueurs considered were found to be greatly affected by the method of preparation and the cultivar/selection of walnuts.

scholarly journals Quantification of rutin and quercetin by HPTLC/HPLC and in vitro immunomodulatory and anticancer activities of Capparis moonii fruits extracts

2017 ◽  
Vol 7 (1) ◽  
pp. 153
Author(s):  
Gaurav Mahesh Doshi ◽  
Manjushree Kundalik Pawar ◽  
Kajal Haribhai Chavda
Keyword(s):  
Gallic Acid ◽  
Cell Lines ◽  
Antibody Titre ◽  
High Performance ◽  
Assay Method ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid ◽  
Rutin And Quercetin

Background: The current research was undertaken on dried fruits of Capparis moonii to screen its potential for immunomodulatory and cancer indications with identification of phytoconstituents by chromatographic techniques.Methods: Methanolic (MECN), hydro-methanolic (HMECN) and aqueous extracts (AQCN) of Capparis moonii were subjected to high performance thin layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) after studying the total phenolic and flavonoid content by using rutin and gallic acid as standards respectively as well as undertaking powder characteristics and preliminary phytochemical screening. Immunomodulatory activities covered were hemagglutination antibody titre and delayed-type hypersensitivity reaction with the aid of sheep red blood cells (0.5×109) as antigens.  The extracts were studied for antioxidant potential. Anticancer prospects were focusing on in vitro cell lines screening (MCF 7 and HCT 15) by Sulforhodamine B assay method and potato disc assay.Results: The total phenolic and flavonoid content of MECM, HMECM and AQCM fruits extracts were found to be 0.20, 0.11 and 0.47 mg of gallic acid/g and 78.3, 18.8 and 64.4 mg of rutin/g respectively. Rutin and quercetin were confirmed by HPTLC and HPLC showing well resolved peaks. IC50 values in antioxidant studies were found to be significant with all the extracts. Significant immunomodulatory effect was noticed at 200mg/kg in both models (high antibody titre levels and decrease paw volume after 48 h). Unsatisfactory results were observed with selected cell lines and disc assay.Conclusions: Thus, selected fruits may probably have immunomodulatory potential due to presence of flavonols (rutin and quercetin).

scholarly journals Utilización de aceite quemado y paraquat en la desecación de soya y sus efectos en la calidad de la semilla durante el almacenamiento

2016 ◽  
Vol 3 ◽  
pp. 61
Author(s):  
R. Ibañez
Keyword(s):  
Environmental Conditions ◽  
Seed Quality ◽  
Rio Grande ◽  
Engine Oil ◽  
Soybean Seeds ◽  
Harvest Time ◽  
Rio Grande Do Sul ◽  
Used Engine Oil ◽  
Soybean Plants ◽  
Germination Vigor

The assay was conducted in Pelotas, Rio Grande do Sul-Brasil, to compare the effect of used engine oil with to paraquat (Gramoxone) applied during the physiological maturition of soybean seeds (Glicyne max (L.) Merrill), Bragg variety. The oil doses were 5.3, 8.5 and 12 l/ha. The desiccation intensity was determined and 30 pods per plot were periodically collected to follow the humidity content of the seeds, to 18%. The humidity was later reduced to 10.5 +0.5 and 2.0 kg of seeds were stored, in cotton bags,during 6 months under the environmental conditions at the Centro de Entrenamiento de Semillas of the Federal University of Pelotas. The seeds were subjected to germination, vigor and phytosanitary tests at harvest time and after 3 and 6 months of storage. Under the conditions of this trial, the doses of 12 l/ha of oil and 2 l/ha of paraquat: a) act as desiccants for soybean plants, b) favors the production of seeds with better viability and vigor after 6 months of storage and c) guarantied better sanitary seed quality after 6 months of storage.

scholarly journals Agronomic Performance of Soybean With Seeds Treated With an Algae Extract Base Biostimulant

2020 ◽  
Vol 13 (1) ◽  
pp. 147
Author(s):  
Gabriel Bressiani Melo ◽  
Alessandro Guerra da Silva ◽  
Adriano Perin ◽  
Guilherme Braga Pereira Braz ◽  
Christiano Lima Lobo de Andrade
Keyword(s):  
Grain Yield ◽  
Block Design ◽  
Natural Compounds ◽  
Soybean Seeds ◽  
Agronomic Performance ◽  
Grain Yields ◽  
Beneficial Effects ◽  
Agronomic Characteristics ◽  
Organic Components ◽  
Randomized Block Design

The use of biostimulants in the treatment of soybean seeds can provide beneficial effects on the crop due to the synergism between the organic components present in products from natural compounds. Due to the scarcity of results of research on biostimulants in the treatment of soybean seeds, the objective of this study was to evaluate the effects of doses of a biostimulant in the treatment of seeds, on agronomic characteristics, productivity components and profitability of soybean culture. Therefore, two experiments were carried out in the field in the 2016/17 and 2017/18 harvests, using a randomized block design with six replications and four treatments referring to doses of 0.00; 0.05; 0.10; and 0.15 L 100 kg seeds-1. The agronomic characteristics of soybeans, grain yield and profitability of using the biostimulant were evaluated. From the obtained results, it can be seen that the dose of 0.15 L 100 kg seeds-1 provided greater profitability in both harvests. In addition, doses above 0.12 L 100 kg seed-1 provide higher grain yields and a higher grain mass, in addition to other productivity components.

Comparison of fatty acid composition of oil from original and regenerated populations of wild Helianthus species

2014 ◽  
Vol 13 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Gerald J. Seiler
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Wild Species ◽  
Oil Quality ◽  
Wild Sunflower ◽  
Breeding Programmes ◽  
Precise Relationship ◽  
Germplasm Accessions

Monitoring and protecting germplasm in genebanks using in situ collections while preserving its original genetic integrity is a priority of germplasm curation. Many germplasm accessions need to be regenerated due to their demand and/or seed condition. The regeneration of wild Helianthus (sunflower) species poses several challenges due to the diversity of 53 wild species. Fatty acid composition of sunflower oil is an important quality factor for the crop. Since oil quality is environmentally influenced, and evaluation of this trait is usually performed on oil from achenes from the original accessions of wild sunflower species, we conducted a study on 72 accessions of eight annual and four perennial taxa of wild sunflower species to compare the oil quality of the original accessions and those regenerated for genebank maintenance. The results showed that the fatty acid composition profiles of achenes from the original and regenerated accessions are not the same. It seems that selection for specific fatty acids in several species will require the analysis of both populations to identify germplasm accessions for use in breeding programmes. It should be borne in mind that accessions of wild species are open-pollinated segregating populations, so one would expect some variability in each succeeding generation. While there may be differences between the original and regenerated accessions, the interrelationships of fatty acids are generally similar in wild and cultivated sunflower species, so there should be no detrimental effects on oil quality when using the wild species for other traits. As more regenerated accessions become available, a more precise relationship between the original and regenerated accessions should emerge.

Sign in / Sign up

Export Citation Format

Share Document