scholarly journals ANTIOXIDANT AND ANTIRADICAL ACTIVITY IN VITRO OF EXTRACTS FROM THE LEAVES OF RUBUS CHAMAEMORUS L. (ROSACEAE)

2021 ◽  
pp. 319-325
Author(s):  
Yana Leonidovna Strakh ◽  
Ol'ga Stepanovna Ignatovets
Keyword(s):  
Phenolic Compounds ◽  
Hydroxyl Radicals ◽  
Raw Materials ◽  
Leaf Extracts ◽  
Spectrophotometric Methods ◽  
Chelating Activity ◽  
Rubus Chamaemorus ◽  
Wide Range ◽  
The Republic

Cloudberry (Rubus chamaemorus L.) is the leader among northern berries in the content of phenolic compounds, which can cause a wide range of biological activity. Currently, there is an increased interest in the study of the antioxidant activity of plant extracts in order to search for promising sources of raw materials for the pharmaceutical and food industries. The article presents the results of a comparative analysis of interpopulation and gender differences in antioxidant and antiradical activities in vitro of cloudberry leaf extracts growing on the territory of the Republic of Belarus. Classical spectrophotometric methods were used for determination: phosphomolibdenic method, Fe + 2-chelating activity, NO-inhibiting activity, inhibition of hydroxyl radicals (OH), method of superoxide radical inhibition in an alkaline solution of dimethylsulfoxide, DPPH, ABTS. Differences in the content of phenolic compounds and flavonoids in the alcoholic extracts of leaf blades of Rubus chamaemorus L. It was found that the population of the Bolshoi Mokh preserve possesses the highest inhibition rates of hydroxyl radicals and superoxide radicals. The maximum iron-chelating activity was exhibited by the extracts of the male plant of the Bolshoy Moh preserve. The least inhibitory activity of Rubus chamaemorus L. leaf extracts was shown to the nitrogen-oxide radical. The established values of antioxidant and antiradical activities, as well as the content of phenolic compounds and flavonoids of the leaf blades of Rubus chamaemorus L. may be important for the development of measures to preserve the species and specific populations on the southern border of the habitat.

scholarly journals COMPARATIVE EVALUATION OF ANTIOXIDANT ACTIVITY OF EXTRACTS OF BERGENIA CRASSIFOLIA (L.) FITSCH LEAVES OF DIFFERENT TERMS OF VEGETATION

2020 ◽  
pp. 231-239
Author(s):  
Svetlana Vladimirovna Tsyrendorzhieva ◽  
Sesegma Dashiyevna Zhamsaranova
Keyword(s):  
Raw Materials ◽  
Antioxidant Properties ◽  
Total Content ◽  
Leaf Extracts ◽  
Green Leaves ◽  
Chelating Ability ◽  
The Republic ◽  
Bergenia Crassifolia ◽  
Maximum Content

New data on the chemical composition and antioxidant properties of aqueous leaf extracts of Bergenia crassifolia (L.) Fitsch of different periods of vegetation growing in the Republic of Buryatia were obtained. It is shown that this is one of the few plants whose leaves contain a significant amount of phenolic compounds. The maximum content of tannins was noted in the extract of red leaves of the incense – 2.42%. Depending on the phase of the collection of raw materials, the content of flavonoids in the water extracts of the leaves of the frangipani varied from 3.88 to 5.42%. A higher accumulation of flavonoids was observed in the extract of black leaves of badan, which is apparently associated with leaf fermentation, in which the cellular structure is destroyed, enzymes and polyphenols are released. The leader in determining the total content of anthocyanins, as well as for flavonoids, was the extract of black leaves of frankincense. The largest amount of arbutin was contained in the extract of green leaves of frankincense – 1.22%. The studied extracts had a high ability to absorb free radicals, however, the extract of green leaves of badan had the highest antiradical activity, had a minimum EC50 of 4 mg/cm3. The iron-chelating ability of the tested aqueous extracts of frankincense leaves of different periods of in vitro vegetation showed that the leaf extracts had weak iron-chelating activity.

scholarly journals Monitoring of the PAL Enzymatic Activity and Polyphenolic Compounds in Leaves and Fruits of Two Myrtle Cultivars during Maturation

Agriculture ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 389
Author(s):  
Silvia Medda ◽  
Leonarda Dessena ◽  
Maurizio Mulas
Keyword(s):  
Phenolic Compounds ◽  
Phenylalanine Ammonia Lyase ◽  
Raw Materials ◽  
Phenylpropanoid Pathway ◽  
Polyphenolic Compounds ◽  
Antioxidant Compounds ◽  
Total Polyphenols ◽  
Spectrophotometric Methods ◽  
Pal Activity ◽  
Potential Use

The leaves and berries of myrtle (Myrtus communis L.) are rich in phenolic compounds, such as phenolic acids, flavonoids, and flavanols. The richness of these antioxidant compounds allows the potential use of myrtle biomasses as raw materials for medicinal and functional food products. Most of the phenolic compounds originate from the phenylpropanoid pathway, where phenylalanine ammonia lyase (PAL) enzyme activates the first step. The objective of this research is to study the activity of PAL as related to accumulation in the myrtle fruits and leaves of some phenolic compounds in the period between blossom and full berry ripening. With this aim, we compared two model genotypes with different fruit coloration. In leaves and berries of two cultivars, ‘Giovanna’ with pigmented berries and ‘Grazia’ with white berries, the PAL activity and content of polyphenols, anthocyanins, flavonoids, and tannins were determined with spectrophotometric methods. PAL activity was quite constant in leaves and variable in berries: Greater in berries of ‘Giovanna’ than in those of ‘Grazia’ cultivar, and increasing from berry color-break to full ripening. In berries, a positive correlation between PAL and flavonoids (r = 0.44), and between PAL and anthocyanins (r = 0.69), as well as a negative correlation between PAL and total polyphenols (r = −0.471), were found.

scholarly journals A novel customized ceramic bracket for esthetic orthodontics: in vitro study

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Liu Yang ◽  
Guangfu Yin ◽  
Xiaoming Liao ◽  
Xing Yin ◽  
Niansong Ye
Keyword(s):  
Shape Matching ◽  
Raw Materials ◽  
In Vitro Study ◽  
Frictional Resistance ◽  
Orthodontic Bracket ◽  
Wide Range ◽  
Ceramic Brackets ◽  
Orthodontic Patients ◽  
Ceramic Bracket

Abstract Background This study aims to develop a novel process to establish a standardized manufacturing technique of customized esthetic ceramic bracket system (CCB) which could be endowed with individual color and shape to satisfy patients’ individual demands. Material characteristics and mechanical parameters of CCB were evaluated. Subjects and methods CCB virtual models were designed individually according to patient’s teeth morphology and clinical demands. 3D printing technology, lost-wax technology, and selected glass-ceramic ingots were employed to fabricate CCB. Scanning electron microscopy (SEM) analyses were performed to characterize the surface morphology of CCB and commercially available brackets (Clarity Advanced; Crystalline VII; Inspire ICE; Damon Q). Static and kinetic frictional resistance (FR), shear bond strength (SBS) and adhesive remnant index (ARI) scores were recorded. One-way analyses of variance (ANOVA) and post-hoc Tukey’s HSD multiple tests were used for statistical analyses. Results Multi-color and multi-transparency raw materials facilitated CCB with a wide range of color options and controllable optical properties to satisfy different esthetic demands of individual orthodontic patients. CCB presented same level of FR as commercially available ceramic brackets did. No significant differences (P ≥ 0.05) of SBS were observed among CCB-ES (treated silane), Clarity Advanced and Crystalline VII groups, and CCB-E (no silane) attained the highest ARI mean score 3. In the preliminary clinical trial, CCB presented excellent color-matching and shape-matching appearances similar to natural teeth, which made it highly invisible from social intercourse distance. Conclusions CCB were demonstrated to be an applicable labial orthodontic bracket system with optimized esthetics and biomechanics. We envision that it would be an ideal alternative for patients who pursue esthetic orthodontic treatment but were not likely to take lingual appliances or clear aligners.

scholarly journals Pleiotropic Biological Effects of Dietary Phenolic Compounds and their Metabolites on Energy Metabolism, Inflammation and Aging

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 596 ◽  
Author(s):  
María del Carmen Villegas-Aguilar ◽  
Álvaro Fernández-Ochoa ◽  
María de la Luz Cádiz-Gurrea ◽  
Sandra Pimentel-Moral ◽  
Jesús Lozano-Sánchez ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Phenolic Compounds ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Biological Properties ◽  
In Vivo Studies ◽  
Wide Range ◽  
High Prevalence

Dietary phenolic compounds are considered as bioactive compounds that have effects in different chronic disorders related to oxidative stress, inflammation process, or aging. These compounds, coming from a wide range of natural sources, have shown a pleiotropic behavior on key proteins that act as regulators. In this sense, this review aims to compile information on the effect exerted by the phenolic compounds and their metabolites on the main metabolic pathways involved in energy metabolism, inflammatory response, aging and their relationship with the biological properties reported in high prevalence chronic diseases. Numerous in vitro and in vivo studies have demonstrated their pleiotropic molecular mechanisms of action and these findings raise the possibility that phenolic compounds have a wide variety of roles in different targets.

scholarly journals MICROCLONAL PROPAGATION OF KARELIAN BIRCH AND TRIPLOID ASPEN IN VITRO

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (2) ◽  
pp. 101-106
Author(s):  
N.S. Anokhina ◽  
◽  
V.F. Konovalov ◽  
E.R. Khanova ◽  
◽  
...  
Keyword(s):  
Tree Species ◽  
Gene Pool ◽  
Raw Materials ◽  
High Quality ◽  
The Republic ◽  
Methodological Aspects ◽  
Karelian Birch

Methodological aspects of microclonal reproduction of valuable tree species – triploid aspen and Karelian birch, which are important for enriching the gene pool of the main forest-forming tree species in the Republic and obtaining high-quality wood raw materials in the form of ornamental and decorative wood, are proposed.

scholarly journals Vegetable growing of the Republic of Kazakhstan in terms of pandemic

2021 ◽  
pp. 101-107
Author(s):  
Y. E. Gridneva ◽  
◽  
G. Sh. Kaliakparova ◽  
◽  
◽  
...  
Keyword(s):  
High Performance ◽  
Agricultural Sector ◽  
Raw Materials ◽  
Public Support ◽  
Global Changes ◽  
Vegetable Production ◽  
Industrial Complex ◽  
Vegetable Crops ◽  
Wide Range ◽  
The Republic

With the spread of the COVID-19 pandemic in most countries, after the adoption of emergency measures to prevent the spread of coronavirus infection, various economic measures have been implemented to support the population and stabilize the economy. Global changes are taking place in the world, which is associated with issues of cooperation, management activities using a wide range of methods, transformations in agro-industrial complex. To ensure food security of the country, the role of agricultural sector of the republic is increasing. The authors explored main directions of the effective development of vegetable growing in the southern region of the country. The issues of vegetable-growing subcomplex of agri-industrial complex have been identified. Based on the analysis, their assessment, as well as the potential of the industry's innovative activity are presented. There are great prospects for vegetable market, as the consumption of these products, which are rich in vitamins, minerals necessary for the organism, as well as a balanced diet, is increasing. The solution to the problems is in the transition to industrial technologies, the widespread dissemination of business ideas. The measures necessary to expand the scope of introduction of high-performance technologies in the vegetable sector are considered. The importance of public support for the growth of vegetable production is shown. The destabilizing factors of competitiveness of vegetable growing in Kazakhstan have been identified, proposals on improvement of the efficiency of this industry have been developed which are based on innovation. The authors state the need for innovative restructuring in breeding and seed production of vegetable crops, public variety testing, and control over the use of pesticides. The level of provision of the population with vegetable products, and processing industry with raw materials are determined by the development and location of vegetable growing in the republic, as well as labor provision and transport accessibility of sales markets.

scholarly journals Effect of in vitro gastrointestinal digestion on phenolic compounds and the antioxidant activity of Aloe vera

2020 ◽  
Vol 7 (3) ◽  
pp. 11-25
Author(s):  
Imen Laib ◽  
Farida Kehal ◽  
Nour Elyakine Haddad ◽  
Taous Boudjemia ◽  
Malika Barkat
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Aloe Vera ◽  
Total Phenol Content ◽  
Gastrointestinal Digestion ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
Total Polyphenol ◽  
Spectrophotometric Methods

AbstractThe aim of this work is to study the effect of digestion on the total polyphenol content, flavonoids and the antioxidant activity of Aloe vera. Total polyphenol contents and flavonoid spectrophotometric methods: The evaluation of the antioxidant activity was carried out by three methods, DPPH, ABTS and CUPRAC. To confirm the results obtained we carried out an analysis by ATR-FTIR. The total phenol content found in the Aloe vera extract studied was 1.3638 mg EAG/100 g, while the content of flavonoids found in the Aloe vera extract studied was 0.690 mg EQ/100 g. The values of total polyphenols and flavonoids decreased under the effect of gastrointestinal digestion. The spectra obtained during the ATR-FTIR analysis show that Aloe vera is rich in phenolic compounds and flavonoids. Intense bands corresponding to O–H bonds, C=C bond, C–H, CO, CH3 and CH2 confirm the presence of these bioactive compounds. For both the DPPH and CUPRAC methods, Aloe vera extract reveals a strong antioxidant activity, which gradually decreases during the oral and gastric phase and then increases after the intestinal digestion. For the ABTS method, the antioxidant activity decreases during the oral phase, increases during the gastric phase and then decreases again during the intestinal phase.

scholarly journals Bioprocessed Wheat Ingredients: Characterization, Bioaccessibility of Phenolic Compounds, and Bioactivity During in vitro Digestion

2021 ◽  
Vol 12 ◽  
Author(s):  
Irene Tomé-Sánchez ◽  
Ana Belén Martín-Diana ◽  
Elena Peñas ◽  
Juana Frias ◽  
Daniel Rico ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Dietary Fiber ◽  
Raw Materials ◽  
Biological Effects ◽  
In Vitro Digestion ◽  
Hydroxycinnamic Acids ◽  
Total Phenolic ◽  
Insoluble Dietary Fiber ◽  
Anti Inflammatory

To enlarge the applications of whole wheat grain (WWG) and wheat bran (WB) as functional ingredients in foodstuffs that can promote human health, researchers have explored bioprocessing approaches to improve the bioaccessibility of phenolic compounds from these food matrices and, subsequently, their biological effects. The objective of this study was to compare the composition in nutrients, anti-nutrients, and bioactive compounds of WWG and WB, and their respective bioprocessed products: sprouted wheat (GERM) and WB hydrolysate (stabilized by spray-drying [SPD] and microencapsulated [MEC]). In addition, to evaluate the functional properties of these ingredients, the bioaccessibility of phenolic compounds and their potential antioxidant and anti-inflammatory activities were monitored in different digestion steps. GERM had increased amounts of insoluble dietary fiber, higher diversity of oligosaccharides, and higher concentration of monosaccharides, free phosphorous, and phenolic compounds than WWG. SPD had improved content of soluble dietary fiber, oligosaccharides, monosaccharides, free phosphorous, and phenolic compounds (vs. WB), whereas MEC was mainly composed of protein and had nearly 2-fold lower content of SPD components. All the ingredients showed lower amounts of phytic acid as compared with raw materials. In all samples, hydroxycinnamic acids were the most representative polyphenols followed by minor amounts of hydroxybenzoic acids and flavonoids. Gastrointestinal digestion of GERM, SPD, and MEC revealed high stability of total phenolic compounds in both gastric and intestinal phases. Hydroxycinnamic acids were the most bioaccessible compounds during digestion among the three bioprocessed wheat ingredients studied, although their bioaccessibility varied across ingredients. In this sense, the bioaccessibility of ferulic acid (FA) derivatives increased in GERM with progression of the digestion, while it was reduced in SPD and MEC up to the end of the intestinal phase. Microencapsulation of SPD with pea protein led to generally to lower bioaccessible amounts of phenolic acids. Comparison analysis of biological effects highlighted SPD for its most potent antioxidant effects in the gastrointestinal tract (3 out 4 antioxidant parameters with highest values), while no clear differences were observed with regard to in vitro anti-inflammatory activity. Overall, these results support the potential application of GERM, SPD, and MEC as functional and nutraceutical ingredients.

scholarly journals Rapid micropropagation, antioxidant and antibacterial assays of Ocimum spp.

2020 ◽  
Vol 49 (3) ◽  
pp. 459-465
Author(s):  
Shamoly Akter ◽  
Barna Goswami ◽  
Salim Khan ◽  
Shahina Akter ◽  
Sanjida Rahman Mollika ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Root Formation ◽  
Shoot Induction ◽  
Leaf Extracts ◽  
In Vitro Micropropagation ◽  
Wide Range ◽  
Half Strength ◽  
Ocimum Tenuiflorum ◽  
High Inhibition

In vitro micropropagation was studied in two species of Ocimum and one species was used for antioxidant and antibacterial activity. The maximum number of shoots/explant (10.1 and 9.5) was induced in MS + 1.0 mg/l BAP + 0.5 mg/l Kn and MS + 2.0 mg/l BAP + 0.5 mg/l IAA in Ocimum tenuiflorum and O. americanum, respectively. GA3 (0.1 mg/l) was found to be effective for shoot induction and elongation in both the species. Strong and stout root formation was observed on half strength MS medium supplemented with 0.2 mg/l IBA for O. tenuiflorum and half strength MS + 0.5 mg/l NAA for O. americanum. Methanolic leaf extract of O. tenuiflorum showed high inhibition of DPPH activity compared to standard antioxidants like quercetin and the chloroform leaf extracts exhibited wide range of antibacterial activity.

Ethnobotany, Phytochemistry and Traditional Uses of Curcuma spp. and Pharmacological Profile of Two Important Species (C. longa and C. zedoaria): A Review

2019 ◽  
Vol 25 (8) ◽  
pp. 871-935 ◽  
Author(s):  
Zahra Ayati ◽  
Mahin Ramezani ◽  
Mohammad Sadegh Amiri ◽  
Ali Tafazoli Moghadam ◽  
Hoda Rahimi ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Animal Studies ◽  
Inorganic Compounds ◽  
Modern Medicine ◽  
Important Species ◽  
Chemical Structures ◽  
Wide Range ◽  
Anti Inflammatory

Ethnopharmacological relevance: The genus Curcuma, which is the most important source of curcumin, has been widely used in different traditional medicines. Various species of Curcuma have long been used for several purposes such as healing wounds, liver disorders, jaundice and also as a blood purifier. Aim of the study: This review focused on the ethnopharmacological uses and phytochemical aspects of Curcuma. Additionally, in this study, the different properties of two species of Curcuma in Islamic Traditional Medicine (ITM), C. longa and C. zedoaria, as well as their pharmacological aspects in modern medicine are reviewed. Materials and methods: ITM literatures were searched to find Curcuma’s applications. Also, electronic databases including PubMed and Scopus were searched to obtain studies giving any in vitro, in vivo or human evidence of the efficacy of C. longa and C. zedoaria in the treatment of different diseases. ChemOffice software was used to find chemical structures. Results: The analysis showed that ethno-medical uses of Curcuma have been recorded for centuries. Approximately, 427 chemical compounds have been isolated and identified from Curcuma spp. This genus is rich in flavonoids, tannins, anthocyanin, phenolic compounds, oil, organic acids and inorganic compounds. Curcumin is one of the main active ingredients in Curcuma which has strong anti-inflammatory and antioxidant effects. Besides, pharmacological studies have indicated wide range of Curcuma’s activities, such as hepato-protective, antifungal, antihypertensive and neuroprotective. Conclusions: In this study, we reviewed various studies conducted on ethno-medicinal, ITM properties and photochemistry of Curcuma spp. Also, pharmacological activities of two species, C. longa and C. zedoaria are summarized. Pre-clinical investigations have demonstrated some of the traditional aspects of Curcuma, such as wound healing, anti-arthritic, anti-tumor and liver protective activities. These could be related to antioxidant and anti-inflammatory properties of Curcuma which might be due to high amounts of phenolic compounds. Curcuma is mentioned to have neural tonic properties in ITM which have been confirmed by some animal studies. Considering various preclinical studies on C. longa and C. zedoaria and their active ingredient, curcumin, randomized controlled trials are warranted to confirm their promise as a clinically effective hepato and neuro-protective agents.

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