scholarly journals Potential of Calliandra calothyrsus Leaf Extract to Maintain Estrogen Concentration and Uterine Thickness in Rats

2021 ◽  
Vol 13 (2) ◽  
pp. 230-236
Author(s):  
Iriani Setyawati ◽  
Ni Nyoman Wirasiti ◽  
Luh Putu Eswaryanti Kusuma Yuni
Keyword(s):  
Leaf Extract ◽  
Endometrial Thickness ◽  
Embryo Implantation ◽  
Uterine Wall ◽  
The Body ◽  
Female Rats ◽  
Control Group ◽  
Calliandra Calothyrsus ◽  
Endogenous Estrogen ◽  
Estrogen Concentration

Calliandra calothyrsus Meissn. leaf extract is potential as phytoestrogens. It influenced male mice reproduction, rat estrous cycle, and ovarian histology in previous study. This research aimed to prove the C. calothyrsus leaf extract potential as phytoestrogen source and the effect on endometrial thickness where the embryo implantation take place in early pregnancy. This study used a Completely Randomized Design used 54 days old female rats (Rattus norvegicus). Rats were divided into K as control group (treated with 0.5% Na-CMC as placebo) and P1, P2, and P3 as three groups with C. calothyrsus leaf extract administration with doses of 17.5; 35; and 70 mg/ kg bw respectively. Treatments were given 1 ml/rat/day orally for 20 days. At day 21st, animals were euthanized to collect blood samples for estrogen hormone analysis. After the dissection, all uterus were collected and weighed. Histological preparation was done with paraffin method and Hematoxylin-Eosin staining. The effective dose was 70 mg/ kg bw that did not decrease the weight of the uterus and the body. This dose even maintained the normal diameter and thickness of uterine walls (endometrium, myometrium, and perimetrium layers) like control rats. The extract in this study could increase estrogen concentration in female rats. This research novelty is that C. calothyrsus leaf extract (70 mg/ kg bw) can be used as an alternative herbal suplement to maintain uterine wall thickness and estrogen concentration in productive women. With further clinical research, this extract is a good candidate as potential estrogen source to overcome women infertility or pregnancy difficulties due to problem of endometrial thickness and lack of endogenous estrogen.

scholarly journals Effects of Red Bit Extract (Beta vulgaris L.) on VEGF, and Endometrial Thickness in Mice (Rattus norvegicus) Exposed to Cigarette Smoke

2018 ◽  
pp. 133-140
Author(s):  
Zulfa Hanum Dahlan ◽  
Dewita Dewita
Keyword(s):  
Cigarette Smoke ◽  
Low Income ◽  
Endometrial Thickness ◽  
Ethanol Extract ◽  
The Body ◽  
Female Rats ◽  
Food Sources ◽  
Control Group ◽  
P Value ◽  
Red Beet

Exposure to cigarette smoke often occurs among people, especially low-income countries. Frequent exposure to cigarette smoke can cause an increase in ROS in the body resulting in increased oxidative stress. As a result of ROS increases the antioxidants in the body decreases, therefore it takes antioxidants from the outside contained in food sources, one red bit. Red bits contain betalain compounds that are high in antioxidants that function to neutralize ROS in the body. This study aims to determine the effect of red beet ethanol extract with various doses to increase the expression of VEGF, and thickness in endometrium of mice exposed to cigarette smoke. The research design is True Experiments with Post Control Only Control Group Design design. The study used 25 female rats divided into 5 groups: negative control group, positive control, treatment I, II and III. The dose of bit extract used was PI (125 mg/kgBW/day), PII (250 mg/kgBW/day), PIII (500 mg/kgBW/day). The data were analyzed using One Way Anova with p-value VEGF (p = 0.000), and p-value of thickness endometrium (p = 0.000). The results showed that administration of red beet ethanol extract at doses of 500 mg/kgBW/day increased the expression of VEGF, and endometrial thickness of mice exposed to cigarette smoke.   Keywords:  Exposure to cigarette smoke, VEGF Expression, Endometrial Thickness, Red Beet.

Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Murtala Akanji Abdullahi ◽  
Elijah Oladapo Oyinloye ◽  
Akinyinka Alabi ◽  
Aderonke Adeyinka Aderinola ◽  
Luqman Opeyemi Ogunjimi ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Laboratory Animals ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Toxicological Evaluation ◽  
Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

scholarly journals Perbedaan Kadar Glutation (GSH) Hepar Tikus Putih Jantan (Rattus norvegicus) yang diinduksi Parasetamol Dosis Toksik dengan Pemberian Ekstrak Daun Kelor (Moringa oleifera)

2020 ◽  
Vol 20 (1) ◽  
pp. 247
Author(s):  
Nur Insani ◽  
H.M.T Kamaluddin ◽  
Swanny Swanny
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Leaf Extract ◽  
The Body ◽  
Control Group ◽  
Bb Rat ◽  
Toxic Dose ◽  
Experimental Animals ◽  
Significant Difference ◽  
Group Ii

Glutathione (GSH) transferase deficiency due to paracetamol exposure causes further oxidative stress to liver necrosis. To reduce oxidative stress that can cause damage to the liver of the body requires antioxidants. One plant to treat liver disease is the kelor leaf (because it has an active flavonoid material also has antioxidant activity). This study was conducted to determine the difference of glutathione hepar levels of male white rat induced paracetamol toxic dose by giving kelor leaf extract. The type of research is experimental laboratory in vivo with rancagan randomized post test only control group design. With the stages as follows 1.Leaf Extract Kelor with Ethanol 96%, 2.Perpeteration of experimental animals, 3.Treatment of experimental animals by giving extract of 3-dose of kelor leaf that is KP I 250 mg / 200 gr BB rat, KP II 500 mg / 200 gr BB rat, KP III 1000 mg / 200 gr BB rat  for 14 days combined with paracetamol dose 2 gr / 200 gr BB rat compared with the negative control group (group given only paracetamol dose 2 gr / 200 gr BB rat) and control group positif only fed regular feed for 14 days). The result showed that there was a significant difference mean of GSH levels between all treatment groups obtained p = 0,000 (p <α) p values smaller than 0.05. There was the highest increase of GSH in treatment group II (142,7525 μmol / mg) and lowest dose of GSH in positive control group (57,1812 μmol / mg), dose paracetamol toxic dosage and kelor leaf extract 500 mg / gr BB rat can increase GSH hepar p = 0,000 (p <α) p less than 0 , 05. The conclusion of the test results showed that giving of kelor leaf extract at dose of treatment group II can increase GSH hepar level significantly

scholarly journals Biokimia Darah Tikus (Rattus norvegicus) Betina Ovariektomi setelah Perlakuan Ekstrak Daun Kaliandra Merah (Calliandra calothyrsus Meissn.)

2019 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Iriani Setyawati ◽  
Ngurah Intan Wiratmini ◽  
Ni Putu Ayu Dewi Wijayanti
Keyword(s):  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Calliandra Calothyrsus ◽  
Biochemical Tests ◽  
Protein Levels ◽  
Blood Biochemical ◽  
Randomized Design ◽  
Completely Randomized Design

Red calliandra (Calliandra calothyrsus Meissn.) leaf have the potential as phytoestrogens. In this study, adult female ovariectomized rats (as a model of menopause women) were given calliandra leaf extract containing estrogenic flavonoids. This study was an experimental design with a Completely Randomized Design (CRD). Forty-five ovariectomized female rats were divided into five groups i.e. untreated control (K), and the treatment group given 80% of C. calothyrsus ethanolic leaves extract doses 25 mg/ kg bw (P1), 50 mg/ kg bw (P2), 75 mg/ kg bw (P3) and 100 mg/ kg bw (P4). The test suspension was given once a day at 24-hour intervals by gavage as much as 0.5 ml/rat/day for two months. At the end of the treatments, blood was collected for blood biochemical tests including hemoglobin levels, hematocrit values, blood glucose and proteins levels. The results showed that C. calothyrsus leaf extract did not negatively affect the health status of the animals through blood biochemical parameters, i.e. hemoglobin levels, hematocrit values, and glucose and protein levels of ovariectomized female rats.

Biosynthetic growth hormone changes the collagen and elastin contents and biomechanical properties of the rat aorta

1991 ◽  
Vol 125 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Annemarie Brüel ◽  
Hans Oxlund
Keyword(s):  
Growth Hormone ◽  
Collagen Type ◽  
Hormone Treatment ◽  
Treated Group ◽  
Collagen Type I ◽  
Biochemical Properties ◽  
The Body ◽  
Female Rats ◽  
Control Group ◽  
Type I

Abstract The biomechanical and biochemical properties of aortas from female rats treated with biosynthetic human GH (b-hGH) for 80 days were investigated. b-hGH was administered at a dose of 5 mg·kg−1·d−1. Treatment with b-hGH increased the body weight by 75% and the diameter of the aorta by 14% compared with the control group. The concentration of collagen and the relative amount of collagen type I were increased, and the concentration of elastin was decreased. Aortas from the b-hGH-treated group showed increased extensibility in the regions corresponding to physiological load values (i.e. 100-200 mmHg), and increased stiffness in regions with higher load values. The increased extensibility at low load values corresponds well with the loss of elastin, and the increased stiffness at higher load values with the increase of collagen and relative increase of collagen type I. These alterations induced by the growth hormone treatment might influence the elasticity and recoiling properties of the aorta.

Effects of lead and/or cadmium on the distribution patterns of some essential trace elements in immature female rats

2011 ◽  
Vol 30 (12) ◽  
pp. 1914-1923 ◽  
Author(s):  
Lin Wang ◽  
Xuelei Zhou ◽  
Dubao Yang ◽  
Zhenyong Wang
Keyword(s):  
Trace Elements ◽  
Inductively Coupled Plasma ◽  
Distribution Patterns ◽  
The Body ◽  
Female Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Inductively Coupled ◽  
Lead And Cadmium ◽  
Essential Trace Elements

Lead acetate (300 mg/L) and/or cadmium chloride (50 mg/L) were administered as drinking water to Sprague-Dawley rats for 9 weeks to investigate the effects of concurrent exposure to lead and cadmium on the distribution patterns of five essential trace elements. Inductively coupled plasma mass spectrometry was used to determine the concentrations of zinc, copper, manganese, selenium and iron in the urine at different exposure times, as well as their levels in the renal cortex and serum at the end of treatment. Compared with the control group, exposure to lead and/or cadmium resulted in a significant increase in the urinary excretion of these five elements during the experiment, whereas significant decreased levels of these elements were found in kidney and serum. In conclusion, increased urinary loss of antioxidant trace elements due to lead and/or cadmium exposure induced the deficiency of antioxidants in the body, which could result in further oxidative damage. Moreover, there was an obvious synergistic effect of lead combined with cadmium on the distribution patterns of these essential trace elements, which may be related to the severity of co-exposure to these two metals.

scholarly journals Impact of lead acetate and sodium and potassium stearates on lipid peroxidation processes in the body of experimental animals

2021 ◽  
Vol 100 (4) ◽  
pp. 406-410
Author(s):  
Olha Ye. Fedoriv ◽  
Alexandra Ye. Kopach ◽  
Nataliia A. Melnyk
Keyword(s):  
Lipid Peroxidation ◽  
Drinking Water ◽  
Lead Acetate ◽  
Sodium Stearate ◽  
The Body ◽  
Female Rats ◽  
White Female ◽  
Control Group ◽  
Diene Conjugates ◽  
Group 2

Introduction. Given the significant prevalence of lead in the environment, research in this area has significant social and economic importance. Lead compounds are characterized by high toxicity and increased ability to cumulate in ecosystems, humans, and animals. Lead enters the human body with food, drinking water, atmospheric air, and smoking. Lead causes pathological changes in the nervous system, blood-forming organs, kidneys, etc. Materials and methods. The experiments were carried out on four groups of white female rats, each included seven animals, weighing 150-200 g. The first group of animals was a control. The second group consumed dechlorinated water from the city water supply, followed by lead acetate. The animals from the third and fourth groups drank the same water with sodium stearate and potassium stearate content in a dose of 1/250 LD50. After the 40th-day of the use of these waters, the animals were orally administered lead acetate at a dose of 7 mg/kg. The levels of lipid peroxidation biomarkers were studied by studying the content of diene conjugates (DC) and malondialdehyde (MDA) in blood serum, liver, and kidney homogenates. Results. The administration of 1/2 acetate LD50 to lead in experimental rats drinking water with stearates was accompanied by a significant increase in the DCs concentration and (MDA) in animals. Higher concentrations of LPO products were observed in the group of animals that consumed water from potassium stearate. Conclusions. 1. With the oral administration of lead acetate against the background of drinking water containing stearates at a dose of 1/250 LD50, an increase in lipid peroxidation indices was noted compared with the control group. 2. Higher concentrations of LPO products were observed in the group of animals consuming water from potassium stearate.

scholarly journals JUMLAH KOLONI CANDIDA ALBICANS PADA PLAT RESIN AKRILIK HEAT CURED SETELAH DILAKUKAN PERENDAMAN EKSTRAK DAUN KEMANGI (OCIMUM BASSILICUM LINN) 50%

2020 ◽  
Vol 16 (2) ◽  
pp. 61-63
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
Sintha Nugrahini
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Acrylic Resin ◽  
The Body ◽  
Control Group ◽  
Control Group Design ◽  
Denture Stomatitis ◽  
Parametric Test ◽  
Group Design ◽  
Cleaning Methods

Accumulation of plaque and food scraps on an acrylic resin base will increase bacterial colonies and C. albicans which will cause denture stomatitis. Maintenance of denture hygiene using mechanical, chemical and combination of two methods. Basil leaf extract contain essential oils which are important in fight against resistant C. albicans biofilms. The purpose of this study was to determine the comparison of C. albicans colonies after cleaning various denture cleaning methods. The method of this study is used an experimental method, the study design is posttest only with control group design. Sample size of heat-cured acrylic resin plate is 40x12x3mm. this study use Kruskall-Wallis test and Mann-Whitney test as data analysis for comparison tests between groups (non-parametric test). The results showed that there were significant differences between the various cleansing methods used in reducing C. albicans colonies. The compotition of flavonoids basil leaf extract is anti-microbial which can prevent the entry of fungi that harm the body.

scholarly journals MORPHOLOGICAL COMPOSITION OF BLOOD AND BIOCHEMICAL PROCESSES IN THE ORGANISM OF FEMALE RATS F1 UNDER LONG-TERM EXPOSURE OF CITRIC ACID

2020 ◽  
Vol 21 (2) ◽  
pp. 183-188
Author(s):  
U. І. Теsarinska ◽  
R. S. Fedoruk ◽  
S. Ya. Маrtynyk ◽  
Т. V. Yurynec ◽  
V. А. Smuk ◽  
...  
Keyword(s):  
Citric Acid ◽  
Biochemical Parameters ◽  
Group Analysis ◽  
Relative Content ◽  
The Body ◽  
Female Rats ◽  
Control Group ◽  
Study Results ◽  
Morphological Composition

The paper considers the effect on rats of long-term feeding of an aqueous solution of citric acid (C6H12O6 x H2O, "chemically pure.") in the amount of 8 mg/l. The study was performed on 10 laboratory female rats F1 of the Wistar line, obtained from F0 females. Two groups were set up: control and experimental, 5 animals in each. Animals were under standard conditions with natural light regime day/night, aqueous solutions of citrate, water, and feed received ad libitum. Were determined the morphological composition and biochemical parameters of the blood of female F1 rats. The results of the study indicate the absence of an unfavorable effect of citric acid on red blood cells. Concerning white blood, there is a decrease in the number of leukocytes, which may be due to the development of an adaptive response of the body to citric acid or the inhibition of citric acid's immune response to long-term adaptive action. There is a significant increase in the relative content of eosinophils in females F1, which probably may be due to the action of citric acid. The analysis of the obtained results of blood biochemical parameters indicates a certain tendency to decrease, in terms of control, the content of serum iron, as well as its total and unsaturated Fe-binding capacity. Ferrum saturation of serum transferrin was present at the level of animals in the control group. Analysis of the study results indicates no changes in control in the activity of enzymes: Alkaline phosphatase ALT and AST, also have no statistically significant confirmation of differences in the control group. The level of urea was statistically not significantly higher in terms of control. Thus, long-term watering of citric acid to female rats in the generation F0 and F1 during their physiological and sexual gestation and pregnancy reveal un-favorable effects in female rats F1. However, there was distinguished the adaptive immune-biological response of the animals organism. The immunological response was supported by a decrease in the number of leukocytes and a significant increase in the relative content of eosinophils. The results of biochemical studies of serum indicate the absence of statistically significant differences in animals of the control group.

scholarly journals Stress-Induced Morphological Changes of Ovarian Histology in Female Wistar Rats

2020 ◽  
Vol 13 (4) ◽  
pp. 1625-1643
Author(s):  
Nwogueze Bartholomew Chukwuebuka ◽  
Ojieh Anthony Emeka ◽  
Ovuakporaye Simon Irukefe ◽  
Wilson Josiah Iju ◽  
Ogbutor Udoji Godsday ◽  
...  
Keyword(s):  
Morphological Changes ◽  
The Body ◽  
Female Rats ◽  
Cellular Damage ◽  
Reproductive Capacity ◽  
Health Concern ◽  
Control Group ◽  
Weight Changes ◽  
Stress Induction ◽  
Ovarian Histology

Stress as it relatesto infertility has become a global issue attracting public health concern. The present study examined the morphological changes in ovarian histology in response to oxidative stress-induced in female rats following exposure to different stressors. 92 rats of 12-14weeks old weighing between 120-160g were used for the study. Three (3) different stress models were utilized for stress induction at the rate of 1, 3, and 5hours per day for 1, 2, and 3weeks respectively. At the end of stress induction durations, bodyweights were obtained and the rats were euthanized via cervical dislocation while the ovary weights were carefully isolated and their weights recorded. The harvested ovary was sectioned, mounted on slides, stained, and observed under the microscope for histopathological investigations. Findings from this study established that exposure to restraint mirror or intruder stressor significantly (p<0.05) altered the body and/or ovary weights of the rats irrespective of the rate of exposure when compared to the control group. Cellular degeneration, infiltration, and atretic follicular changes were observed in the ovarian histology of rats in response to stress-inducedchanges caused by exposure to restraint or intruder stressors, whereas, equivalent exposure of the rats to mirror stressor did not result in any observed degenerative changes in the histology of the ovary studied. Our study revealed that exposure to restraint or intruder stressor points towards the existence of stress contributes towardsorgan/body weight changes and cellular damage inthe ovarian tissuespossiblycausing pathogenesis in reproductive capacity of females.

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