scholarly journals GCMS AND TLC ANALYSIS OF ETHANOLIC EXTRACT OF SEED MOMORDICA CHARANTIA

2021 ◽  
Vol 9 (11) ◽  
pp. 313-318
Author(s):  
T. Purushoth Prabhu ◽  
◽  
Shanthini Naichyar ◽  
Sheela a ◽  
Kavitha b ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Standard Procedure ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Octadecanoic Acid ◽  
Conjugated Linolenic Acid ◽  
Good Source ◽  
Defense Systems ◽  
Tlc Analysis ◽  
Endogenous Defense

To perform the TLC and GCMS analysis of ethanolic extract of seed Momordica charantia. The seeds ofMomardicacharantia was extracted with ethanol and tested for TLC and GCMS by standard procedure as per the guidelines of WHO.The phytochemical and TLC studies showed the presence of alkaloids, cardiac glycosides, phenols, steroids, terpenoids and proteins.The GCMS analysis of seed Momardicacharantiashowed five major compounds were found to be n-hexadeconic acid, 9,12-Octadecadinoic acid,Octadecanoic acid,Hexadecanoic acid-2-hydroxy-1-hydroxy methyl ethyl ester and Gamma-sitosterol.The Therapeutic activity may be due to the major compounds identified by GCMS and further have to be evaluated.Mostly cucurbitaceae plant possesses the highest nutritive value and also a good source of carbohydrates, proteins, fibers, vitamins, and minerals. Fruits are composed of sufficient of water,protein and lipids respectively.In addition to this MC seeds can represent a good source of lipids, such as polyunsaturated fatty acids and they are among the few foods containing conjugated linolenic acid, being as eleostearic acid. The essential oil, obtained from drought seeds, contains sesquiterpenes, phenylpropanoids and monoterpenes. Other bioactive compounds, such as tocopherols and polyphenols have been reported in general. The pericarp, the aril, the stem and the leaves of the plant are also a good source of phenolic compounds, which can be useful to protect from oxidative damage by acting directly on reactive oxygen species and to induce endogenous defense systems.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

Magneto-Priming Improved Nutraceutical Potential and Antimicrobial Activity of Momordica charantia L. Without Affecting Nutritive Value

2019 ◽  
Vol 188 (3) ◽  
pp. 878-892
Author(s):  
Shazia Anwer Bukhari ◽  
Nabila Farah ◽  
Ghulam Mustafa ◽  
Saqib Mahmood ◽  
Syed Ali Raza Naqvi
Keyword(s):  
Antimicrobial Activity ◽  
Nutritive Value ◽  
Momordica Charantia

scholarly journals Phytochemical, physicochemical, TLC, minerals analysis and in-vitro antioxidant activity of ethanolic extract of leaves of Heldigardia populifolia

2018 ◽  
Vol 1 (1) ◽  
pp. 22-26
Author(s):  
T Bhanumathi ◽  
P Keerthana ◽  
A Cheenakesavulu ◽  
M Neeharika ◽  
E Sandhya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Permissible Limits ◽  
Leaf Powder ◽  
Composition Of Minerals ◽  
Tlc Analysis ◽  
In Vitro Antioxidant Activity

The aim of present study was to investigate the preliminary phytochemical, physicochemical, TLC, minerals analysis and In-vitro antioxidant activity of leaves of ethanolic extract of Heldigardia populifolia. The preliminary phytochemical screening of ethanolic extract showed the presence of triterpenoids, flavonoids, glycosides, sterols, steroids, phenols, carbohydrates and saponins. The composition of minerals found in the leaf powder was within the permissible limits. TLC analysis of ethanol extract showed the five spots which indicate the presence of five phytoconstituents. The extractive value of ethanol was high than acetone. Ash values were within the limits. The in-vitro antioxidant activity of ethanolic extract increased with increasing the concentration. The ethanolic extract in all the concentration showed the significant antioxidant activity.

scholarly journals Antioxidant Potential and Stabilization Studies of Sunflower Oil Using Sorbaria tomentosa Extract and its Cu(II)/Zn(II) Chelates

2020 ◽  
Vol 70 (12) ◽  
pp. 4193-4200
Keyword(s):  
Sunflower Oil ◽  
Total Phenolics ◽  
Storage Period ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Emulsion System ◽  
Synthetic Antioxidants ◽  
Good Source ◽  
Linoleic Acid Emulsion ◽  
Β Carotene

Sorbaria tomentosa,, commonly known as “Berre” is native to Himalaya and Hindukush range in Pakistan. Qualitative phytochemical screening as well as quantitative antioxidant potential of its ethanolic extract was evaluated. Antioxidant potential of the extract was determined using standard methods like DPPH, FRAP, total phenolics, total flavonoids, ABTS radical cation scavenging assay and β-carotene linoleic acid emulsion system. Cu(II) and Zn(II) chelates of ,,Sorbaria tomentosa,, were also prepared and their antioxidant potential was compared with the extract as well as with synthetic antioxidants (BHT and BHA). It was observed that ,,Sorbaria tomentosa,, is a good source of natural antioxidant that worked efficiently compared to respective chelates. Keeping in view less efficiency of chelated extracts, stabilization studies of sunflower oil were conducted with ethanolic extract (250, 500, 1000 ppm) of ,,Sorbaria tomentosa,,. Various parameters like PV, FFA and IV were estimated to evaluate stabilization of oil. ,,Sorbaria tomentosa,, extract (1000 ppm) showed almost same role during stabilization like BHA at ambient condition during a storage period of 45 days. Keywords: Sorbaria tomentosa, phytochemicals, antioxidant potential, chelation, stabilization

scholarly journals The effect of bitter gourd (Momordica charantia) ethanolic extract on inflammatory infiltrates and NF-κB activation in periodontitis

2019 ◽  
Vol 1 (1) ◽  
pp. 6
Author(s):  
Aryudhi Armis ◽  
Tetiana Haniastuti ◽  
Heni Susilowati
Keyword(s):  
Alveolar Bone ◽  
Inflammatory Responses ◽  
Ethanolic Extract ◽  
Inflammatory Cells ◽  
Momordica Charantia ◽  
Bitter Gourd ◽  
Tnf Α ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
Inflammatory Infiltrates

Periodontitis is a periodontal disease involving the gingiva, periodontal ligament, cementum, and alveolar bone due to an inflammatory process. Virulence factors of periodontopathogens and inflammatory responses in periodontitis can stimulate nuclear factor-kappa B (NF-κB) activity. Charantoside c and Momordicosides g in bitter gourd prevent NF-κB activation stimulated by TNF-α in HepG2 cells. This study aims to determine the effect of bitter gourd fruit (Momordica charantia) ethanolic extract as an anti-inflammatory substance on the level of inflammatory infiltrates and the number of cells that experience NF-κB activation in the periodontitis model. Eighty male Wistar rats were divided into 5 groups. The mandibular incisors were ligated for 14 days to induce periodontitis. Each group was given Momordica charantia extract of 500 mg/kg BW, 250 mg/kg BW, and 100 mg/kg BW; ibuprofen 100 mg/kg BW; and aquades orally using oral gavage on the day 14. Rat necropsy was carried out on day 1, 3, 5, and 7 after giving the substances. Taking out the lower jaw was done to make tissue preparations followed by staining them with hematoxylin eosin (HE). Immunohistochemicalanalysis was performed to observe cells that were positive for NF-κB activation. The results showed a decrease in the density of inflammatory infiltrates in all groups, except for those given aquades. The number of inflammatory cells ofneutrophils, macrophages, and lymphocytes that experienced NF-κB activation showed the most effective decrease in the group of Momordica charantia 500 mg/kg BW, on the 7th day. The conclusion of this study is that ethanolic extractof Momordica charantia has an anti-inflammatory effect and prevents the activation of NF-kB in rat gingival induced by periodontitis. The highest effect was found at a dose of 500 mg/kg BW on day 7 after giving of extracts.

scholarly journals Evaluation of Antioxidant Capacity, Protective Effect on Human Erythrocytes and Phenolic Compound Identification in Two Varieties of Plum Fruit (Spondias spp.) by UPLC-MS

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3200 ◽  
Author(s):  
Karen L. Hernández-Ruiz ◽  
Saul Ruiz-Cruz ◽  
Luis A. Cira-Chávez ◽  
Laura E. Gassos-Ortega ◽  
José de Jesús Ornelas-Paz ◽  
...  
Keyword(s):  
Protective Effect ◽  
Antioxidant Capacity ◽  
Ethanolic Extract ◽  
Ultra Performance Liquid Chromatography ◽  
Human Erythrocytes ◽  
Compound Identification ◽  
Good Source ◽  
Edible Part ◽  
Biological Interest

Plum edible part was used to obtained extracts by during a 4 h maceration process using three different solvents (ethanol, methanol and water) for the determination of total phenols and flavonoids, antioxidant capacity by (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hemolysis inhibition in human blood assays. Subsequently, phenolic compounds were identified using ultra-performance liquid chromatography (UPLC-MS). The results indicated that the ethanolic extract of plum fruit being a good source of phenolic (12–18 mg GAE/g FW) and flavonoids (2.3–2.5 mg QE/g FW) content in both varieties of plum. Also, the fruits proved a good source of antioxidants as measured by DPPH and ABTS; likewise, plum aqueous extracts showed the highest protective effect on human erythrocytes with 74.34 and 64.62% for yellow and red plum, respectively. A total of 23 bioactive compounds were identified by UPLC-MS, including gallic acid, rutin, resorcinol, chlorogenic acid, catechin, and ellagic acid, and the antioxidant capacity can be attributed to these species. The edible part of plum contains compounds of biological interest, suggesting that this fruit has antioxidant potential that can be exploited for various technologies.

scholarly journals Analysis of Bioactive Chemical Compounds of Leaves Extracts from Tamarindus indica Using FT-IR and GC-MS Spectroscopy

2021 ◽  
pp. 22-34
Author(s):  
Mansour Abdulnabi H. Mehdi ◽  
Abdul-hakim M. A. Al- Alawi ◽  
Ahmed Zain A. Thabet ◽  
Fadel Y. S. Alarabi ◽  
Gozif Mohammed N. Omar ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Vaccenic Acid ◽  
Octadecenoic Acid ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Tamarindus Indica ◽  
Octadecanoic Acid ◽  
Leaf Extracts ◽  
Phytochemical Compounds ◽  
Ft Ir

Tamarindus indica is one of the medicinal plants used in the treatment of various diseases traditionally. Aims: This study was conducted to identify the phytochemical constituents of T. indica leaf extracts. Methods: Using Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography-mass spectrometry (GC-MS) to identification of bioactive compounds in extracts of T. indica. Results: The FT-IR spectrum confirmed the presence of alcohol group, alkene group, amine group, carbonates, ethers, carboxylic acid and disulfides in both extracts. A total of 22 and 38 bioactive phytochemical compounds were identified in the ethanolic and aqueous extracts of T. indica, respectively. The major bioactive compounds of the ethanolic extract of T. indica leaves were cis-Vaccenic acid, trans-13-Octadecenoic acid, Oleic Acid, Octadecanoic acid, Octadecanoic acid, 2-(2-hydroxyethoxy) ethyl ester, Eicosanoic acid and Eicosane, 1-Iodo-2-methylundecane, 10-Methylnonadecane. While the major bioactive compounds of the aqueous extract were 3-O-Methyl-d-glucose, Myo-Inositol, 4C-methyl-, Myo-Inositol, 2-C-methyl-, Propane, 2,2-dimethoxy-, 1,3-Dioxolane, Ethanol, 2-(1-methylethoxy)-, and 2-Pentanone, 4-hydroxy-4-methyl-, 2-Hexanol, 2-methyl-, Ethanamine, N-methyl.

scholarly journals Potensi Ekstrak Etanol Daun Pare (Momordica charantia L.) sebagai Alternatif Obat Penyembuh Luka pada Punggung Mencit Jantan (Mus musculus L.)

2017 ◽  
Vol 17 (2) ◽  
Author(s):  
Muhammad Pazry ◽  
Hendri Busman ◽  
Nuning Nurcahyani ◽  
Sutyarso Sutyarso
Keyword(s):  
Wound Healing ◽  
Mus Musculus ◽  
Povidone Iodine ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Male Mice

The leaves of bitter melon (Momordica charantia L.) contains flavonoid, tannin and saponin which plays a role in wound healing. The purpose of this study was to determine the effect of bitter melon leaves (M. charantia L.) ethanolic extract on wound healing the back injury on male mice. The parameters of this study were the length of wound closure and histopathological observations by looking at the level of epithelialization, the number of new blood vessels, and the number of inflammatory cells.This study was conducted in a complete randomized design. The mice were divided into five groups: [K (+)], [K (-)], [ P1], [P2], [P3], each group consist four mice. All of mice was wounded by cut on the back skin about 1,5 cm in length. The wound were treated every two times a day for 9 days, K(+) is given povidone iodine as a positive control, K(-) is given 95% ethanol as a negative control, P1 is given 50% ethanolic extract of bitter melon, P2 is given 75% ethanolic extract of bitter melon and P3 is given 100% ethanolic extract of bitter melon. On the 10th day, one of the mice from all of groups was taken to observe the histopathology of their skin. The results showed that the leaf extract of bitter melon has wound healing potential by accelerate the healing process of back skin’s wound in mice and has best histopathology picture compared to controls. Keywords : Bitter Melon (Momordica charantia L.), Male Mice (Mus musculus L.), povidone iodine

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