Antioxidant and Enzyme Inhibitory Properties of Mangifera indica leaf Extract

2020 ◽  
Vol 10 (4) ◽  
pp. 384-394
Author(s):  
Sainiara Begum ◽  
Archana Banerjee ◽  
Bratati De
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Mangifera Indica ◽  
Agricultural Waste ◽  
Ethyl Acetate Fraction ◽  
Soil Amelioration ◽  
Liver Disorders ◽  
Inhibitory Activities

Aims:The foliar residues of Mangiferaindica tree are usually burned or used for soil amelioration except nominal uses as fodder.Methods:To add value to this agricultural waste, extracts of the leafy residues of M. indica were studied to analyze their potential as antioxidants and to inhibit the enzymes related to the management of diabetes, Alzheimer’s Disease (AD), hepatic disorders as well as to identify important phytochemicals present in the extracts.Results:Results depicts that the leaves have notable bioactivities. The methanol extract (ME) showed much potential than ethyl acetate fraction after hydrolysis (HME) against α-amylase and α- glucosidase. The activity against the enzyme β-glucuronidase was also higher than that of the commercial β- glucuronidase inhibitor. The extract after hydrolysis showed better antioxidant and acetylcholinesterase inhibitory activities. Detection of important phytochemicals such as chrysin and myricetn, alizarin, arbutin, hydroquinone, tyrosol, taxifolin, kaempferol, mangiferin, and the vitamin alpha tocophereol, in addition to a number of organic acids, amino acids, fatty acids, sugars and polyols by GC-MS and HPTLC based analysis of the extract of M. indica leaf, also suggest the use of the leaves as sources of these important phytochemicals.Conclusion:More concisely HME with more number of detected metabolites found better to be used against oxidative stress as well as enzymes related to neural and liver disorders than that of ME.

scholarly journals Mangifera indica L. Leaf Extract Induces Adiponectin and Regulates Adipogenesis

2019 ◽  
Vol 20 (13) ◽  
pp. 3211 ◽  
Author(s):  
Giuseppe Sferrazzo ◽  
Rosa Palmeri ◽  
Luca Vanella ◽  
Lucia Parafati ◽  
Simone Ronsisvalle ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Lipid Metabolism ◽  
Lipid Content ◽  
Leaf Extract ◽  
Mangifera Indica ◽  
Adipogenic Differentiation ◽  
Obese Patients ◽  
Intracellular Lipid ◽  
Expression Of Genes

Natural bioactive compounds may be used in obese patients because of their ability to impact on various key mechanisms involved in the complex pathophysiological mechanisms of such condition. The aim of this study was to investigate the effect of a Mangifera indica L. leaf extract (MLE) on adipogenic differentiation of murine preadipocyte cells. 3T3-L1 cells were treated during their differentiation with various concentrations of (Mangifera indica L.) leaves extract (MLE) (750, 380, 150, 75 and 35 μg) in order to assess their lipid content, adiponectin production, expression profile of genes involved in lipid metabolism, oxidative stress and inflammation. Our results showed that MLE was particularly enriched in polyphenols (46.30 ± 0.083 mg/g) and that pharmacological treatment of cells resulted in a significant increase of adiponectin levels and reduction of intracellular lipid content. Consistently with these results, MLE resulted in a significant decrease of the expression of genes involved in lipid metabolism (FAS, PPARG, DGAT1, DGAT2, and SCD-1). In conclusion, our results suggest that MLE may represent a possible pharmacological tool for obese or metabolic syndrome patients.

scholarly journals Antimicrobial and Antioxidant Studies of the Leaf Extract and Fractions of Sabicea brevipes Wernham (Rubiaceae)

2019 ◽  
pp. 1-11
Author(s):  
Chimaobi O. Ugorji ◽  
Janefrances N. Ihedioha ◽  
Matthias O. Agbo ◽  
Nwachukwu R. Ekere ◽  
Felix I. Nwafor ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Significant Difference

Phytochemical analysis, antimicrobial and antioxidant properties of the leaf extract and fractions of Sabicea brevipes were studied. The leaves were defatted and the marc extracted with methanol. The extract was further purified by solvent-solvent partitioning using n-hexane, ethyl acetate and n-butanol to obtain the three solvent fractions. They were screened for phenolics, flavonoids, tannins, saponins, terpenoids, glycosides, and steroids. Total phenolics, flavonoids and tannins were determined quantitatively. The antimicrobial test was screened in vitro by agar diffusion method. Analysis of variance (ANOVA) was used to test for significant difference at p ≤ 0.05 in all study groups. The methanol extract exhibited the most significant amount of phenolics (110.78 ± 1.06 mg GAE/g) while ethyl acetate fraction had the least total phenolics content (50.55 ± 2.91 mg GAE/g). The same trend was observed for the total flavonoids content whereas the methanol extract measured (418.40 ± 14.03 mg QE/g) while ethyl acetate fraction had 192.40 ± 3.06 mg QE/g. Total tannins contents were: methanol extract (102.22 ± 7.58 mg GAE/g) and ethyl acetate (27.33 ± 0.77 mg GAE/g). The antioxidant results showed that the methanol extract had the highest DPPH free radical scavenging ability (93.69%) with half maximal inhibitory concentration (IC50) of 0.601± 0.02 and also highest ferric ion reducing power (50.381 ± 1.56 µmol Fe2+/g). Also, the methanol extract showed high total antioxidant capacity (96.79 ± 0.31 mg AAE) and IC50 of 0.798± 0.01. The antimicrobial results revealed that the methanol extract showed better activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans than the solvent fractions at concentrations of 200, 100, 50, 25, 12.5, mg/mL having various inhibition zone diameters (IZDs). The methanol extract and fractions of S. brevipes compared favourably in terms of zone of inhibition and minimum inhibition concentration (MIC) with the standard drug disc (Gentamycin and Ketoconazole) against the tested microorganisms. The MIC of the extract and solvent fractions ranged from 6.31 mg/mL to 50.12 mg/mL. The continual use of the extract of Sabicea brevipes in preventing oxidative stress and in the treatment of common infection is justified by these results.

Antioxidant and acetylcholinesterase inhibitory activities of leaf extract and fractions of Albizia adianthifolia (Schumach) W.F. Wright

2017 ◽  
Vol 28 (2) ◽  
Author(s):  
Mubo A. Sonibare ◽  
Ibukun O. Ayoola ◽  
Taiwo O. Elufioye
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phenolic Contents ◽  
Inhibitory Activities ◽  
Polar Extracts ◽  
Albizia Adianthifolia ◽  
Gallic Acid Equivalent

AbstractBackground:This study was conducted to determine the antioxidant and acetylcholinesterase inhibitory activities of the leaf extract and different fractions ofMethods:The antioxidant and acetylcholinesterase inhibitory activities of the methanol extract (CME), ethyl acetate (EAF), chloroform (CHF), andResults:Phytochemical screening revealed important classes of secondary metabolites. All extracts showed good antioxidant and acetylcholinesterase inhibitory activities. The EAF and CHF gave the highest total phenolic contents of 18.02±0.14 and 18.43±0.95 mg gallic acid equivalent per gram of extract, respectively. The polar extracts gave the highest activity in both assays with lower ICConclusions:The results of this study suggest that the leaf of

Effects of Papaya Leaf Extract (Carica papaya L.) on Cellular Proliferation and Apoptosis in Cervical Cancer Mice Model

2019 ◽  
Vol 17 (5) ◽  
pp. 265-275
Author(s):  
Y. Peristiowati ◽  
Y. Puspitasari ◽  
Indasah
Keyword(s):  
Cervical Cancer ◽  
Cell Proliferation ◽  
Hela Cells ◽  
Leaf Extract ◽  
Caspase 3 ◽  
Methanol Extract ◽  
Negative Control ◽  
Proliferation Index ◽  
Control Group ◽  
P53 Expression

This study is aimed at analyzing the anticancer properties of papaya leaf extract, specifically the inhibition of cell proliferation and apoptotic induction through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and p53 pathways. Twenty-five mice (Mus musculus), aged 2 months and weighing 20–30 g, was injected with 0.5 mg dexamethasone for 7 days. The mice were then injected intracutaneously with 1 ml of HeLa cells (8 × 106 HeLa cells/microliter). The mice were divided into five groups (5 each): negative control (P1) (5% CMC-Na, sodium carboxymethyl cellulose), treatment II (225 mg/kg BW (body weight) papaya leaves methanol extract), treatment III (450 mg/kg BW), treatment IV (750 mg/kg BW), and treatment PV (2 mg alcohol anticancer drug). Papaya leaf extract treatments were applied for 2 weeks. Then, the tumor tissue was isolated for hematoxylin and eosin staining. Immunohistochemical imaging was used to detect Ki-67, caspase-3, NF-κB, and p53 expression. Further analysis was undertaken using the ImmunoRatio software program. The results indicated that administration of papaya leaf methanol extract significantly increased the expression of NF-κB and p53 at a dose of 450 mg/kg BW. Our results also showed that the mice treated with 450 mg of papaya leaf extract per kg of BW (P3) had the largest increase of caspase-3 expression compared to the negative control group. Papaya leaf ethanol extract decreased the cancer cell proliferation index and increased apoptosis of cancer cells in animal models of cervical cancer; it may also work to increase NF-kB expression and expression of the p53 gene.

FORMULATION AND CHARACTERIZATION OINMENT OF ARUM MANIS MANGO (Mangifera indica L.) LEAVES METHANOL EXTRACTS AS AN ANTIBACTERIAL AGAINST Propionibacterium acnes

2020 ◽  
Vol 16 ◽  
Author(s):  
Dian Riana Ningsih ◽  
Zusfahair Zusfahair ◽  
Dadan Hermawan ◽  
Wulan Anggraeni ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Methanol Extract ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Sweat Glands ◽  
Ointment Formulation ◽  
Inhibition Zones ◽  
Mango Leaves ◽  
Dispersive Power

Background: Acne is caused by several factors including the active secretion of sebaceous sweat glands, hyperkeratosis in the hair infundibulum and the effects of bacteria. One of the plants that has the potential as an antibacterial is the extract of arumanis mango leaves. Method: Determine the Minimum Inhibitory Concentration (MIC) of methanol extract of mango leaves which can inhibit Propionibacterium acnes activity. The antibacterial activity tests were performed using agar diffusion. The ointment formulation, the characteristics of ointment preparations and the ointment activity against P. Acnes are discussed. Result: MIC of methanol extract of mango leaves value is 5 ppm with an inhibition zone of 1 mm. The ointment obtained is white, has distinctive smell, semisolid form, possesses a pH of 4.92 - 5.87, dispersive power of 5.05 - 6.30 cm, adhesive power of 1 - 3.67 seconds, homogeneous and protective. Ointment preparations of methanol extract of mango leaves has activity on P. acnes on the 0 and 15th day of storage. The activities of ointment preparation on day 0 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 10.20 mm; 19.97 mm and 23.60 mm respectively, while the inhibition zones produced by the preparation of ointment on day 15 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 5.71 mm; 9.58 mm and 21.88 mm respectively. Conclusion: Methanol extract of mango leaves (Mangifera indica L.) and oinment preparation are able to inhibit the growth of Propionibacterium acnes.

scholarly journals Amino Acid and Acylcarnitine Levels in Chronic Patients with Schizophrenia: A Preliminary Study

Metabolites ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 34
Author(s):  
Irina A. Mednova ◽  
Alexander A. Chernonosov ◽  
Marat F. Kasakin ◽  
Elena G. Kornetova ◽  
Arkadiy V. Semke ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Amino Acid ◽  
Chronic Schizophrenia ◽  
Integrated Approach ◽  
Serum Levels ◽  
Tandem Mass ◽  
Chronic Patients ◽  
Healthy Donors ◽  
Preliminary Study

Amino acids and acylcarnitines play an important role as substrates and intermediate products in most of pathways involved in schizophrenia development such as mitochondrial dysfunction, inflammation, lipid oxidation, DNA damage, oxidative stress, and apoptosis. It seems relevant to use an integrated approach with ‘omics’ technology to study their contribution. The aim of our study was to investigate serum amino acid and acylcarnitine levels in antipsychotics-treated patients with chronic schizophrenia compared with healthy donors. We measured serum levels of 15 amino acids and 30 acylcarnitines in 37 patients with schizophrenia and 36 healthy donors by means of tandem mass spectrometry. In summary, patients with chronic schizophrenia had an altered concentration of a few amino acids and acylcarnitines in comparison to the healthy probands. Further research is needed to assess and understand the identified changes.

Can keto/amino acids reduce oxidative stress in peritoneal dialysis patients with hypoalbuminemia?

2021 ◽  
Author(s):  
Erdem Çankaya ◽  
Yusuf Bilen ◽  
Abdullah Uyanık ◽  
Hasan Dogan ◽  
Ahmet Kızıltunç ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Peritoneal Dialysis ◽  
Dialysis Patients
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