scholarly journals Formulasi Sediaan Krim Anti Acne dan Uji Aktivitas Ekstrak Etanol Bawang Dayak (Eleutherine bulbosa (Mill.) Urb) terhadap Propionibacterium acnes

2019 ◽  
Vol 2 (2) ◽  
pp. 121-126
Syahrida Dian Ardhany ◽  
Yunari Puspitasari ◽  
Yuyun Meydawati ◽  
Susi Novaryatiin

Bawang dayak (Eleutherine bulbosa (Mill.)urb) is one of plants of Central Kalimantan. Empirically, Bawang dayak is used to treat skin problems, one of them is acne vulgaris, so that it is necessary to test the inhibition of ethanolic extract of Bawang Dayak against acne causing such as P.acnes.  In the pharmaceutical world there is still no research conducted on the manufacture of antiacne cream contain ethanolic extract of Bawang Dayak bulbs, so the researchers conducted it to improve the efficiency of the use of traditional medicine. The results of phytochemical screening showed that ethanolic extract of bawang dayak contained flavonoid, alkaloid, saponin, tanin and catechol with the inhibition zone classified as moderate activity at the concentration of 1% and weak activity at other concentrations (5%, 10% and 15%). Based on the evalution of cream preparations the results of the organoleptic test for antuacne cream have a dark brown color and distinctive odor, homogen for homogeneity test, adhesion test and dispersion test according to the requirements and the pH observation showed ph (6) suitable for topical application. Based on these results, the evaluation antiacne cream fulfills the physical test, but it needs to be developed further for stability and effectiveness of antiacne cream against the bacteria acnes causing.   


Objective: The aims of this present study were to formulate antiacne cream consisting ethanolic extract of bawang dayak and evaluate antibacterialactivity of cream on day 0 and day 7 to see stability activity and preparation.Methods: Cream formula of bawang dayak was evaluated organoleptic characteristics, homogeneity, pH, adhesion test, dispersion test, and in vitroantibacterial against Propionibacterium acnes.Results: The results of evaluate cream homogeneity were F3 and F4 on day 7 separate and non-homogen but all formula pH suitable for topicalapplication. On day 0, F1 and F2 shown antibacterial activity in category weak activity, F3 and F4 in category moderate activity, while on day 7 allformula have antibacterial activity in category weak activity.Conclusion: All cream formula potentials inhibit against P. acnes but this research must be improved both of preparation and stability activity.


Objective: The aim of the present study was to investigate phytochemical screenings and the in vitro effect antibacterial of BD (Eleutherine Sp.) and TU (Ampelocissus Sp.) against Propionibacterium acnes.Methods: The antibacterial activity was investigated against Propionibacterium acnes by well diffusion method.Results: Preliminary phytochemical screenings of BD ethanolic extract were found positive alkaloid, saponin, tannins, and steroid while TUpositive flavonoid, saponin, tannins, steroid, and triterpenoid. Antibacterial activity against Propionibacterium acnes of ethanolic extract BD with concentration 25 mg/ml and 50 mg/ml showed the zone of inhibition 3.23 mm and 7.8 mm with category weak activity while ethanolic extract TU with same concentration showed zone of inhibition 10 mm (weak activity) and 16.3 mm (moderate activity) which mean ethanolic extract TU have better antibacterial activity. A combination ethanolic extract of both with variant ratio showed a zone of inhibition 6.7 mm (1:1), 3.9 mm (1:2), and 3.63 mm (2:1).Conclusion: In this present study showed the highest potential antibacterial activity against propionibacterium acnes is an ethanolic extract of TU and The best ratio combination is 1:1. Furthermore, this study needs more research with variant concentration so that may be possible to be used as natural anti-acne formulations.

Yusufu, W. N. ◽  
Suleiman, H. O. ◽  
Akwa, V. Y. ◽  
David, D. L. ◽  
Taiga, A.

Propionibacterium acnes (P. acnes) isolated in infections of deep tissues was originally thought to be as a result of improper sterilization of skin and hence contamination of medical processes with this commensal/ normal flora of the skin. P. acnes has latter been identified as the principal agent responsible for the progression of some deep tissue diseases. The antimicrobial susceptibility patterns between different strains of P. acnes was determined. Ten (10) P. acnes clinical isolates of infections were considered, two (2) from acne vulgaris and eight (8) [two (2) per recA types 1A1, 1B, II and III] from lumber herniation tissues. Antibiotic susceptibility of some antibiotics was also determined and resistance to tetracycline was seen in 1 isolate, trimethoprim (1 isolate) and weak activity seen in 5 isolates. Heteroresistance to rifampicin was witnessed among 6 isolates. Upon four days of incubation, no inhibition of the bacterial growth by tetracycline in isolate 1(III). Isolate 17(1B) had no inhibition from trimethoprim. There was low inhibition of P. acnes by this antibiotic in isolates 71(IA1), 82(IB), 55(II), 1 (III) and 64(III). Amoxicillin and rifampicin created greater zones of inhibition among P. acnes isolates. Erythromycin was the next most inhibiting antibiotic with greater inhibition zones. Although Rifampicin had large inhibition zones, heteroresistance was observed among isolates 82(IB), 24(II), I (III), 17(IB), 55(II) and 71(IA1) as colonies were observed growing within the zones after 4 days of incubation. The antibiotics resistance expressed in the study were seen displayed among the lumber disc herniation isolates only with none involving acne vulgaris isolates. This study supports the theory that most P. acnes isolates in deep tissues exhibit higher rate of antibiotic resistance. P. acnes hence might be named the etiological agent of foreign-body infections like infections of indwelling medical devices.

2019 ◽  
Vol 891 ◽  
pp. 52-59
Chanai Noysang ◽  
Teerarat Pummarin

Benchalokawichian (BLW) remedy is a Thai traditional medicine that has been notified in the List of Medicine Products of the National List of Essential Drugs A.D. 2006 and has long been used as an antipyretic. The phytochemicals of the 70% and 95% ethanolic extracts of were studied by color reaction test and HPTLC analysis. The BLW remedy showed a highest amounts of 70% ethanolic extractives (ca. 3.99 ± 0.60% of dry material weight). The several ethanolic extracts showed similar qualitative phytochemicals. The major of phytochemicals identified in these extracts were alkaloids, flavonoids, tannins, and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analysed 70% ethanolic extract showed a highest antioxidant activity at IC50= 526.09 µg/ml and both extracts not showed tyrosinase inhibitory effect. The 95% ethanolic extract showed the greatest anti-propionibacterium acnes activity with inhibition zone of 10.10 ± 0.45 mm and minimum inhibitory concentration (MIC) was 5.05 mg/ml

2022 ◽  
Vol 21 (1) ◽  
pp. 84-89
Fitri A Nurani ◽  
Nadia RS Rejeki ◽  
Tanti Setyoputri ◽  
Putri K Wardani ◽  
Fatkhan B Ridwan ◽  

Introduction: Corn silk or known as herb name stigma maydis is an important medicinal botanical in many traditional medicines worldwide, including jamu, an Indonesia traditional medicine. The exploration of corn silk to treat acne vulgaris is still lacking, therefore the current research was conducted to analyze the activity of ethanolic extract from corn silk (EECS) against 3 acne-related bacteria, Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus. Materials and Methods: The antibacterial activity of EECS at concentration range of 10 to 100% v/v was evaluated using the disk diffusion method. As comparation, distilled water was used as a solvent control, while 1% clindamycin was used as a positive control. Results: Shinoda’s test showed that flavonoid was detected in the EECS. The higher concentration of EECS exhibited higher diameter of inhibition zone indicating higher antibacterial activity on P. acnes, while the antibacterial activity of S. epidermidis was not increased at similar concentrations of EECS. The antibacterial activity of EECS against S. aureus decreased at the higher EECS concentration (>70%). Conclusion: Taken together, EECS is a potential as a bioactive source to inhibit the growth of acne-related bacteria P. acne, S. epidermidis and S. aureus. Further investigation is needed to explore the corn silk or stigma maydis as a medicinal botanical in jamu targeted to treat acne vulgaris. Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 84-89

2013 ◽  
Vol 10 (1) ◽  
pp. 152-160 ◽  
Baghdad Science Journal

The antibacterial activity of some extracts of A. eupatoria (aqueous and ethanolic) against some pathogenic bacteria (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli ) and their activity on wound healing in rats , also the presence of some active compounds in both extracts were detected . The results showed that the ethanolic extract was more effective on inhibiting tested bacteria than the aqueous extract . P.aeruginosa was the most resistant bacteria, while highest inhibition zone appeared on E.coli (20 mm) .There was a moderate activity against S.aureus with inhibition zone 15 mm. by using ethanolic extract (10 mg/ml) . The phytochemical analysis for detection of active compounds revealed the presence of Carbohydrates, Glycosides and Tannins in both extracts, while some of compounds such as Terpenoids and Phenolic compounds (flavonoids) were detected in the ethanolic but not in the aqueous extracts. Prepared ethanolic extract ointment presented obvious activity on wound healing activity in rats in contrast with fucidin ointment and aqueous extract ointment, hence the wound healing was completed in l0 days by using the ethanolic extract ointment, while it was 12 days and 14 days for the aqueous extract ointment and fucidin ointment respectively, in comparison with the untreated wound which needed more than 16 days for healing completion.

Sains Medika ◽  
2016 ◽  
Vol 6 (2) ◽  
pp. 56
Indri Kusuma Dewi ◽  
Titik Lestari ◽  
Siti Nur Rofi'ah

Introduction: Andrographis paniculata had known contained active substance such as andrographolida, atsiri oil, flavonoid, tannin, alkaloid, and saponin which functions as antibacterial, antitoxic, analgesic, and anti-pyretic. Based on its antibacterial properties, an ointment form of Andrographis paniculata leaf is then formulated, for practical usage. Ointment formula is semi solid for topical use in the skin or mucosal membranes. Objectives: to know the result of ointment physical test of etanolic extract Andrographis paniculata leaf. Methods: physical tests contain organoleptic test, pH, homogenity, adhesion test and dispersive test. Results: Ointment shaped semi solid, blacky green and special smell of Andrographis paniculata, pH 6, homogenity test obtained homogenous results, adhesion test was 82 second and dispersive test was 5.6 cm. Conclusion: the result of physical tests of etanolic Andrographis paniculata leaf extract ointment appropriate with quality standard.

2018 ◽  
Vol 1 (1) ◽  
pp. 20-26
Anom Parmadi

Injuries are lost or damaged part of the body's tissues. Wounds are also defined as physical damage due to the opening or destruction of the skin which can cause abnormal skin function and anatomy. These plants widely used traditionally from fresh noni leaves used as a medicine for broken bones, cuts, burns and pain by boiling and drinking the juice. These studys to make use of more effective and efficient Noni leaf extract made in cream preparations as a wound medicine, so that people are easy to use. This study used an experimental research method, namely the cream formulation of noni concentrated ethanol extract of 5%, 10% and 15%. Cream preparation evaluation test included: Organoleptic, pH test, cream type test, homogeneity test, sticky power test, dispersion test and protection power test. The test method was carried out on mice by means of cream preparations applied to the back of mice which were given an incision wound. Its effectiveness is seen from the measurement of wound length for seven days to calculate the percentage of wound healing power. The results of the One Way ANOVA test showed that there were significant differences between concentrations of 5%, 10% and 15% with significant values <0.05. The percentage of healing power of noni leaf extract cream concentration of 5% = 7,69%, concentration of 10% = 17,26%, and concentration of 15% = 29,35%. The results showed that the ethanol extract of noni leaves can be made in cream dosage form and has the effectiveness to accelerate the healing of incision wounds in mice.

2021 ◽  
Vol 4 (1) ◽  
pp. 36
Indriyani Arman,Hosea Jaya Edy, Karlah L.R Mansauda

AbstractMiana leaves (Coleus Scutelleroides (L.) Benth.) contained flavonoid compounds that has antioxidants properties. This study aimed to formulate peel-off gel mask ethanol extract from Miana leaves became peel-off gel mask that is physically stable and to knows the type of base that form a formulation with good physical quality based on the test parameters of physical properties and the stability of preparation. Formulation  of peel-off gel mask ethanol extract of miana leaves used three different types of base, they are HPMC, Carbopol, and Na.CMC. Each formula is differentiated based on base of concentration which were HPMC 4%, carbopol 1%, and Na.CMC 3%. Evaluation to predict the physical stability of the preparation included organoleptic test, homogeneity test, pH test, dispersion test, adhesion test and dry time test. In stability test, each formula is placed at temperature 27 oC for 28 days and made observation on the 1st, 7th, 14th, 21th, and 28th day. Based on the result, the ethanol extract of Miana leaves can be formulated into a peel-off mask and formulation with HPMC and Carbopol base has a good physical quality and physically stable compared to Na.CMC base.Keywords: Miana Leaves, Peel-Off Gel Mask, Stability Teh  AbstrakDaun Miana (Coleus Scutelleroides (L.) Benth.) memiliki kandungan senyawa flavonoid yang berkhasiat sebagai antioksidan. Penelitian ini bertujuan untuk memformulasikan ekstrak etanol daun Miana menjadi masker gel peel-off yang stabil secara fisik serta mengetahui jenis basis yang membentuk formulasi sediaan masker dengan mutu fisik yang baik berdasarkan parameter uji sifat fisik dan stabilitas sediaan. Formulasi sediaan masker gel peel-off  ekstrak etanol daun Miana dibuat dengan menggunakan tiga jenis basis yang berbeda yaitu HPMC, karbopol, dan Na.CMC. Masing-masing formula dibedakan berdasarkan basis konsentrasi yaitu HPMC 4%, karbopol 1%, dan Na.CMC 3%. Evaluasi yang dilakukan terhadap stabilitas fisik sediaan masker gel peel-off meliputi uji organoleptis, uji homogenitas, uji pH, uji daya sebar, uji daya lekat, dan uji waktu mengering. Uji stabilitas dilakukan pada suhu kamar (27 oC) selama 28 hari dan dilakukan pengamatan pada hari ke 1, 7, 14, 21, dan 28. Hasil uji stabilitas fisik sediaan masker gel peel-off ekstrak etanol daun Miana dapat diformulasikan dalam bentuk sediaan masker gel peel-off , dan formula masker dengan basis HPMC dan karbopol memiliki mutu fisik yang baik dan stabil secara fisik dibandingkan dengan basis Na.CMC.Kata kunci: Daun Miana, Masker gel peel-off, Uji Stabilitas.

2021 ◽  
Vol 1 ◽  
pp. 1035-1045
D Dananirroh ◽  
Urmatul Waznah ◽  
W Wirasti ◽  
S Slamet

AbstractMusa paradisiaca Linn, also known as the banana plant in Indonesia, is a herbaceous plant that belongs to the Musaceaa family. Cotton banana peel (Musa paradisiaca Linn.) can be used as a wound medicine. For use, it is still simple, namely by applying the inside of the banana peel to the injured body part, and by scraping the banana peel from the inside to bandage the wound. For this reason, it is necessary to develop a dosage form of this cotton banana peel so that it is easier and provides comfort in its use. Purpose: To find out the skin of cotton banana (Musa paradisiaca Linn) is formulated in cream preparations. The method in this study was experimental, the sample was extracted by maceration using 96% ethanol as solvent. The viscous extract obtained was used at concentrations of 0.08%, 0.10%, and 0.12%. The results showed that cotton banana peel extract (Musa paradisiaca Linn) could be made as a cream and fulfilled the physical evaluation of the preparation. The results of the homogeneity test were that the preparations were made homogeneous, the pH of the cream was obtained at pH 5-7 still fulfilling the skin pH range of 4-7, the adhesion test results obtained more than 5 seconds, the dispersion test results obtained 6-7 cm, the viscosity test at formula I is 3564 – 4253 cPas, Formula II is 4042 – 4746 cPas, Formula III is 4466 – 5254 cPas. The conclusion of this study, the formulation of the ethanol extract cream of cotton banana peel (Musa paradisiaca Linn) met the requirements for physical evaluation of the preparation including homogeneity test, pH test, organoleptic test, dispersibility test, adhesion test, viscosity test and stability test.Keywords: Formulation, banana peel, cream, evaluation. AbstrakMusa paradisiaca Linn atau dikenal dengan nama tumbuhan pisang di Indonesia adalah tumbuhan herba yang termasuk dalam keluarga Musaceaa. Kulit buah pisang kapas (Musa paradisiaca Linn.) dapat digunakan sebagai obat luka. Untuk penggunaan masih sederhana yaitu dengan mengoleskan bagian dalam dari kulit buah pisang pada bagian tubuh yang luka, dan dengan cara mengorek kulit buah pisang dari dalam untuk membalut luka. Untuk itu perlu adanya pengembangan bentuk sediaan dari kulit pisang kapas ini sehingga lebih memudahkan dan memberikan kenyamanan dalam penggunaannya. Tujuan Untuk mengetahui kulit buah pisang kapas (Musa paradisiaca Linn) diformulasikan dalam sediaan krim. Metode pada penelitian ini dilakukan secara eksprimental, sampel di ekstraksi dengan cara maserasi dengan menggunakan pelarut etanol 96%. Ekstrak kental yang diperoleh digunakan pada konsentrasi 0.08%, 0.10%, dan 0.12%. Hasil penelitian menunjukkan bahwa ekstrak kulit pisang kapas (Musa paradisiaca Linn) dapat dibuat sebagai krim dan memenuhi evaluasi fisik sediaan. Hasil uji homogenitas bahwa sediaan yang dibuat homogen, pH krim diperoleh pH 5-7 masih memenuhi kisaran pH kulit 4-7, uji daya lekat hasil yang diperoleh lebih dari 5 detik, uji daya sebar hasil yang diperoleh 6 – 7 cm, uji viskositas pada formula I yaitu 3564 – 4253 cPas, Formula II yaitu 4042 – 4746 cPas, Formula III yaitu 4466 – 5254 cPas. Kesimpulan pada penelitian ini, formulasi sediaan krim ekstrak etanol kulit pisang kapas (Musa paradisiaca Linn) memenuhi syarat evaluasi fisik sediaan meliputi uji homogenitas , uji pH, uji organoleptis, uji daya sebar, uji daya lekat, uji viskositas dan uji stabilias.Kata kunci: Formulasi, kulit pisang, krim, evaluasi.

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