scholarly journals Formulasi dan evaluasi karakter fisik sediaan gel ekstrak etanol daun salam (Syzygium polyanthum)

2021 ◽  
Vol 2 (1) ◽  
pp. 16-22
Author(s):  
Lalu Mukhlis Maqbul Sani ◽  
Windah Anugrah Subaidah ◽  
Yayuk Andayani
Keyword(s):  
Free Radicals ◽  
Physical Properties ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Methyl Paraben ◽  
Freeze Thaw ◽  
Gel Preparation ◽  
Antioxidant Effects

Abstrak: Bay leaves (Syzygium polyanthum) are known to have antioxidant effects that can neutralize free radicals found in the skin. The objectives of this research are to formulate the ethanol extract of bay leaves into gel formulation and evaluate the physical properties of gel formulation. Ethanol extract of bay leaves was obtained through the maceration method with 70 % ethanol as a solvent. The thick ethanol extract of bay leaves was formulated into gel formulation with CMC – Na as a gelling agent, glycerin as a humectant, methyl paraben as a preservative, and aquadest as a solvent. After the freeze-thaw test was done, there was no change found in organoleptic but there were changes in pH, spreadability, and adhesion. The pH of gel preparation before being in storage was 5 and after being in storage was 6. In the spreadability, gel formulation spread 5,5 cm before being in the storage, and 5,1 cm after being in the storage. Gel adhesion has a sticky time of 8.35 seconds before in being storage and 8.45 seconds after being in storage.

scholarly journals PENGARUH VARIASI GELLING AGENT KARBOMER 934 EKSTRAK ETANOL DAUN JERUK PURUT (Citrus hystrix DC) TERHADAP SIFAT FISIK GEL DAN AKTIVITAS ANTIBAKTERI Staphylococcus aureus

2019 ◽  
Vol 4 (1) ◽  
pp. 248-259
Author(s):  
Anggun Hari Kusumawati ◽  
Siti Hufi Hutami
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Physical Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Gelling Agent ◽  
Topical Product ◽  
Gel Preparation ◽  
Made In

ABSTRAK Ekstrak etanolik daun jeruk purut (Citrus hystrix D.C) mempunyai banyak zat khasiat dimana salah satu zat aktif tersebut berkhasiat sebagai antibakteri. Penggunaan ekstrak etanolik dalam bentuk kental sangat tidak efisien dalam pemakaiannya, sehingga perlu dibuat dalam bentuk sediaan topical, misalnya gel. Penggunaan Karbomer 934 sebagai gelling agent dapat meningkatkan konsistensi basis yang akan berpengaruh terhadap pelepasan zat aktif di dalam gel. Penelitian ini bertujuan untuk mengetahui pengaruh variasi kadar basis Karbomer 934 gel ekstrak etanolik daun jeruk purut terhadap sifat fisik gel dan aktivitas antibakteri terhadap Staphylococcus aureus. Gel dibuat dalam 4 formula dengan konsentrasi basis Karbomer 934 yaitu 0,5 %, 1%, 1,5%, dan 2%. Gel diuji sifat fisik (organolepstis, homogenitas, pH, viskositas, daya lekat, daya sebar, dan uji hedonik) dan daya antibakteri terhadap Staphylococcus aureus. Data dari uji sifat fisik dan pengukuran diameter zona hambat anti bakteri dianalisis dengan statistika Uji Kruskall-Wallis dan Mann-Withney Hasil penelitian dapat disimpulkan bahwa variasi konsentrasi Karbomer 0,5 %, 1 %, 1,5 % dan 2% sebagai gelling agent gel ekstrak etanol daun jeruk purut berpengaruh terhadap sifat fisik gel dan aktifitas antibakteri terhadap Staphylococcus aureus. Semakin besar konsentrasi Karbomer 934, viskositas semakin besar dengan FI= 3079 cP.s, FII= 8135 cP.s, FIII= 13136 cP.s, FIV= 14307 cP.s, menurunkan daya sebar dengan FI= 5.14 cm, FII= 4.04 cm, FIII= 3.51 cm, FIV= 3.35 cm, meningkatakan daya lekat dengan FI= 0.79 cm, FII= 0.87 cm, FIII= 1.05 cm, FIV= 1.16 cm dan menurunkan efektivitas antibakteri dengan FI= 6.4 cm, FII= 5.1, FIII= 5.8 cm dan FIV= 4.9 cm. Kata Kunci : gel, gelling agent, Karbomer 934, Citrus hystrix D.C , Staphylococcus aureus.   ABSTRCT Ethanolic extract of kaffir lime leaves (Citrus hystrix D.C) Has many active compounds, antimicrobial activity have been reported numerously. Ethanolic extract of Kaffir Lime leaves can’t directly exposure to the human skin. It can be increased of skin rash and eritema. The extract can be formulated in topical product like gel preparation. The aim of this work was to determine the effect of variations in base levels of Karbomer 934 gel ethanolic extract of kaffir lime leaves on the physical properties of gel and antibacterial activity against Staphylococcus aureus. The gel is made in 4 formulas with a base concentration of Carbomer 934 which is 0.5%, 1%, 1.5%, and 2%. Gel was tested for physical properties (organolepstis, homogeneity, pH, viscosity, adhesion, dispersion, and hedonic test) and antibacterial power to Staphylococcus aureus. Measuring the diameter of the antibacterial inhibition zone after incubation at 37ºC for 24 hours. Data from the physical properties test and measuring the diameter of the anti-bacterial inhibitory zone were analyzed by statistical with Kruskal-Wallis test and Mann-Withney test. The results of this study concluded that variations in Carbomer 934 concentration as gelling agent gel ethanol extract of kaffir lime leaves had an effect on the physical properties of the gel and antibacterial activity against Staphylococcus aureus. The greater concentration of Carbomer 934, increase the viscosity value with FI= 3079 cP.s, FII= 8135 cP.s, FIII= 13136 cP.s, FIV= 14307 cP.s, decrease the spreadability with FI= 5.14 cm, FII= 4.04 cm, FIII= 3.51 cm, FIV= 3.35 cm, increase in adhesion with FI= 0.79 cm, FII= 0.87 cm, FIII= 1.05 cm, FIV= 1.16 cm and decrease the effectiveness of the antibacterial with FI= 6.4 cm, FII= 5.1, FIII= 5.8 cm dan FIV= 4.9 cm. Keywords: gel, gelling agent, Carbomer 934, Citrus hystrix D.C , Staphylococcus aureus.

scholarly journals Pengaruh gelling agent kombinasi karbopol dan hpmc terhadap sifat fisik sediaan gel ekstrak etanol daun bandotan (ageratum conyzoides l) The effect of gelling agent combination of carbopol and hpmc on the physical properties of the preparation of bandotan

2021 ◽  
Vol 1 ◽  
pp. 59-66
Author(s):  
Intan Siswi Saputri ◽  
Dwi Bagus Pambudi ◽  
Wirasti Wirasti ◽  
St Rahmatullah
Keyword(s):  
Physical Properties ◽  
Extraction Method ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Laboratory Research ◽  
Ageratum Conyzoides ◽  
Qualitative And Quantitative ◽  
Dispersion Test ◽  
Gel Preparation ◽  
Collection Methods

AbstractGelling agent is a base in gel preparations that are used to thicken and make drug preparations or cosmetic preparations stable. Natural ingredients have been widely used because they are efficacious as medicine. One of them is bandotan plant (Ageratum Conyzoides L). This study aims to determine the effect of the combination of gelling agent carbopol and HPMC on the physical properties of the gel preparation of bandotan leaf ethanol extract. In this study 3 formulations of gel preparations of bandotan leaf ethanol extract were made with different concentrations of carbopol and HMPC . This research is an experimental research and uses qualitative and quantitative data collection methods from laboratory research. The extraction method used in this study is the maceration method with 96% ethanol as solvent. The prepared gel preparations were tested including organoleptic tests, pH tests, adhesion tests and dispersion tests. The results of this study indicate that there is an effect on the gelling agent of the combination of carbopol and HPMC on the gel preparation of the ethanol extract of bandotan leaves (Ageratum conyzoides L) in formula III with a ratio of gelling agent carbopol and HPMC 0.5%: 6% which is the best gel seen. from pH test, dispersion test and adhesion test.Keywords: Ageratum Conyzoides L; gel; karbopol; hpmc; gelling agent AbstrakGelling agent merupakan basis dalam sediaan gel yang digunakan untuk mengentalkan dan menjadikan sediaan obat atau sediaan kosmetik tetap stabil. Bahan alam sudah banyak digunakan karenaberkhasiat sebagai obat. Salah satunya adalah tumbuhan bandotan (Ageratum Conyzoides L). Penelitian ini bertujuan untuk mengetahui adanya pengaruh kombinasi gelling agent karbopol dan HPMC terhadap sifat fisik sediaan gel ekstrak etanol daun bandotan. Dalam penelitian ini dibuat 3 formulasi sediaan gel ekstrak etanol daun bandotan dengan perbedaan konsentrasi karbopol dan HMPC. Penelitian ini merupakan penelitian experimental dan menggunakan metode pengumpulan data kualitatif dan kuantitatif dari penelitian laboratorium. Metode ekstraksi yang digunakan dalam penelitian ini adalah metode maserasi dengan pelarut etanol 96%. Sediaan gel yang telah jadi dilakukan pengujian diantaranya uji organoleptis, uji pH , uji daya lekat danuji daya sebar. Hasil dari penelitian ini menunjukkan bahwa terdapat pengaruh padagelling agentkombinasi karbopol dan HPMC terhadap sediaan gel ekstrak etanol daun bandotan (Ageratum conyzoides L) pada formula III dengan perbandingan gelling agent karbopol dan HPMC 0,5% : 6% yang merupakan gel paling baik dilihat dari uji pH , uji daya sebar dan daya lekat.Kata Kunci : Ageratum Conyzoides L; gel; karbopol; hpmc; gelling agent

scholarly journals OPTIMIZATION OF CARBOPOL 940 AND PROPYLENE GLYCOL CONCENTRATION ON THE CHARACTERISTIC AND INHIBITORY EFFECT OF ETHANOL EXTRACT GEL OF PAPAYA (Carica papaya L.) SEEDS AGAINST Staphylococcus aureus

2021 ◽  
Vol 18 (1) ◽  
pp. 15-25
Author(s):  
Octavianus Yandri ◽  
Wahyuning Setyani
Keyword(s):  
Staphylococcus Aureus ◽  
Physical Properties ◽  
Propylene Glycol ◽  
Inhibitory Activity ◽  
Carica Papaya ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Activity Test ◽  
Carbopol 940 ◽  
Papaya Seed

Papaya (Carica papaya L.) seeds contain alkaloids, flavonoids, tannins, phenolic compounds and saponins have been proven its synergistic effect in inhibiting the growth of Staphylococcus aureus. In this research, ethanol extract of papaya seeds was formulated in gel preparations. Optimization of the composition of the gelling agent and humectant was carried out to obtain the gel preparation of papaya seed ethanol extract with good physical properties and stability. The parameters used to determine the stability of the preparation are Physical properties which include viscosity, spreadability, and percentage of viscosity shift. Data analysis was performed using Design-Expert software version 12 and SPSS. The inhibitory activity test was carried out by the disk-diffusion agar method with Staphylococcus aureus ATCC 25923 as the test bacteria. The results of the inhibitory activity test of papaya seed ethanol extract at a concentration of 20% had moderate activity and at concentrations of 40%, 60%, 80% and 100% classified as strong against the Staphylococcus aureus. Carbopol 940 is dominant factor in influencing the response of viscosity (92.504%) and spreadability (59.539%). Preparations with good physical properties and stability were obtained on the use of carbopol 940 and propylene glycol as much as 1.06604 grams and 13.2146 grams respectively.

scholarly journals Studi Variasi Gelling Agent PVA (Propil Vinil Alkohol) pada Formulasi Masker Peel-Off Ekstrak Lidah Buaya (Aloe vera) sebagai Anti Jerawat

2019 ◽  
Vol 10 (2) ◽  
pp. 245
Author(s):  
Dias Ardini ◽  
Pudji Rahayu
Keyword(s):  
Physical Properties ◽  
Staphylococcus Epidermidis ◽  
Univariate Analysis ◽  
Aloe Vera ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Adhesive Properties ◽  
Drying Time ◽  
Study Evaluation ◽  
Ph Range

<p><span>Gel mask can increase skin moisture and enhance the effect of the main composition (Aloe vera L) as an anti-acne on the epithelial and that is the cause of occlusivity of the polymer layer formed. PVA formulation is used to give exfoliated effect because they have adhesive properties or can form films that are easily peeled off after drying. The purpose of this study was to determine the effect of polyvinyl alcohol (PVA) variations as a gelling agent (7%, 10%, 13% and 16%) on the physical properties of ethanol extract Aloe vera leaves skin gel mask included organoleptic, homogeneity, pH, dispersibility, drying time and inhibition test against Staphylococcus epidermidis. The research conducted is experimental. The design of this research is a one-shot case study. Evaluation results were analyzed using univariate analysis. For physical properties, frequency distribution and inhibitory tests were analyzed with Analysis of variance (ANOVA). Peel off gel mask is formulated with 4 treatments and 6 repetitions. The results showed that the peel-off gel mask of Aloe vera leaves ethanol extract have clear organoleptic properties, very weak alcohol flavor and 50% slightly thick and 25% thick and 25% very thick. 75% of mask preparations are homogeneous. Has a pH range of 5.95-6.2. The spreadability of 75% gel mask preparations are meet the requirements of mask masking and 75% meets the requirements of good drying time. All variations of the formula have an inhibition effect against Staphylococcus epidermidis and between training groups, no difference was understood.</span></p>

scholarly journals FORMULASI SEDIAAN GEL NANOPARTIKEL LIPID EKSTRAK DAUN BI-NAHONG (Anredera cordifolia (Ten.) Steenis)

2020 ◽  
Vol 3 (2) ◽  
pp. 40
Author(s):  
Rini Dwiastuti ◽  
Shinta Elvina Ardiyati
Keyword(s):  
Physical Properties ◽  
Leaf Extract ◽  
Water Solubility ◽  
Physical Stability ◽  
Lipid Nanoparticles ◽  
Stability Test ◽  
Soy Lecithin ◽  
Freeze Thaw ◽  
Lipid Nanoparticle ◽  
Gel Preparation

ABSTRACT Binahong leaf extract has low water solubility. To increase the solubility of binahong leaf extract, it is necessary to formulate lipid nanoparticle gel dosage form. Lipid nanoparticles are preparations that can deliver both hydrophilic and lipophilic drugs. This study aims to make a gel lipid nanoparticles from binahong leaf extract with the physical properties parameters of the gel preparation. This research is a  experimental study with independent variables of the concentration of binahong extract and variables depending on the size of the lipid nanoparticles and the physical properties of the gel. Gel making was done by using a variation of the amount of extract 5% and 10%. Testing the physical properties of the gel includes organoleptic, viscosity, dispersibility, pH, homogeneity, and syneresis. The physical stability test carried out is accelerated stability test using the freeze thaw cycling method for 3 cycles. Based on the results of the PSA test, of the two formulas made, one formula fulfills the range 50-100 nm. After testing the physical properties of the gel, the two formulas had a dispersion capacity within the 3-5 cm range, were homogeneous, and did not occur syneresis. The results of the viscosity test showed that the two formulas did not meet the 20-30 Pa.s. The pH test results showed that the two formulas did not meet the pH range 4.5-6.5. Key word: binahong; lipid nanoparticle; soy lecithin, gel ABSTRAKEkstrak daun binahong memiliki kelarutan dalam air yang rendah. Untuk meningkatkan kelarutan ekstrak daun binahong, perlu dilakukan formulasi sediaan dalam bentuk gel nanopartikel lipid. Nanopartikel lipid merupakan sediaan yang dapat membawa obat hidrofilik maupun lipofilik. Penelitian ini bertujuan untuk membuat gel nanopartikel lipid ekstrak daun binahong yang  memenuhi parameter sifat fisis sediaan gel. Penelitian ini merupakan eksperimental murni dengan variabel bebas konsentrasi ekstrak binahong dan variabel tergantung ukuran nanopartikel lipid dan sifat fisis gel. Pembuatan gel dilakukan dengan menggunakan variasi jumlah ekstrak 5% dan 10%. Pengujian sifat fisis gel meliputi organoleptis, viskositas, daya sebar, pH, homogenitas, dan sineresis.. Uji stabilitas fisis yang dilakukan adalah uji stabilitas dipercepat dengan metode freeze thaw cycling selama 3 siklus. Berdasarkan hasil uji PSA, dari dua formula yang dibuat, satu formula memenuhi rentang 50-100 nm. Setelah dilakukan uji sifat fisik gel, kedua formula memiliki daya sebar sesuai rentang 3-5 cm, homogen, dan tidak terjadi sineresis. Hasil uji viskositas menunjukkan kedua formula tidak memenuhi rentang 20-30 Pa.s. Hasil uji pH menunjukkan kedua formula tidak memenuhi rentang pH 4,5-6,5. Kata Kunci: binahong; nanopartikel lipid; soy lecithin, gel

scholarly journals Formulasi dan Karakterisasi Sediaan Nanoemulgel Serbuk Lidah Buaya (Aloe Vera L.)

2019 ◽  
Vol 16 (1) ◽  
pp. 28-37
Author(s):  
Teguh Imanto ◽  
Roseh Prasetiawan ◽  
Erindyah Retno Wikantyasning
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Physical Properties ◽  
Physical Stability ◽  
Aloe Vera ◽  
Tween 80 ◽  
Gelling Agent ◽  
Freeze Thaw ◽  
Particle Size Analyzer ◽  
Oil In Water

Aloe vera L. contains antiseptic saponins and anthraquinone complexes as antibacterial. This research was conducted to formulate aloe vera powder into nanoemulgel, characterize and test its physical properties, and to know the effect of gelling agent concentration (carbopol and chitosan) on nanoemulgel. Emulsion type used is oil in water (O / W) with surfactant tween 80 and co-surfactant propylene glycol. Nanoemulsion is characterized by percent of transmittance with UV-Vis spectrophotometer, also potential droplet and zeta size distribution with Particle Size Analyzer. Hydrogels consist of 4 combination formulas of carbopol 0.5%; 1%; 1.5%; 2% and 0.3% chitosan. The formula is tested for physical properties including organoleptic, pH, spreadability, adhesion, viscosity and stability with freeze thaw method. The results showed that the nanoemulsion droplet size was 65.05nm ± 13.49 with zeta potential of -0.1mV and the percent of transmittance above 98%. The result of physical properties of the four formulas shows that the different gelling agent concentration gives different physical properties of viscosity and spreadability. Overall, formula 3 has the best physical properties and physical stability compared to formula 1, 2 and 4.

scholarly journals Optimization Carbopol And Glycerol As Basis Of Hand Gel Antiseptics Extract Ethanol Ceremai Leaf (Phyllantus Acidus (L.) Skeels) With Simplex Lattice Design

2017 ◽  
Vol 2 (01) ◽  
pp. 35 ◽  
Author(s):  
Dwi Saryanti ◽  
Izzatun Ni'mah Zulfa
Keyword(s):  
Physical Properties ◽  
Ethanol Extract ◽  
Gelling Agent ◽  
Test Results ◽  
Lattice Design ◽  
Simplex Lattice Design ◽  
Carbopol 940 ◽  
Phyllanthus Acidus ◽  
Simplex Lattice ◽  
The One

Ceremai leaf (<em>Phyllanthus acidus </em>(L.) Skeels) is the one of the potential plant which contain flavonoid that had antibacterial activity. Carbopol is a gelling agent that is easily dispersible in water and provide consistency to the gel, while the glycerin is a humectant that can improve the softness of the gel. Carbopol can provide viscosity / hardness in the gel it is necessary to combine with glycerin to soften the gel and the increase of the spread The aims of this research to know the optimum composition from carbopol and gliserol in antiseptic gel formulation of ceremai leaf ethanol extract by Simplex Lattice Design (SLD). The method of extraction used maseration method with ethanol 70 % solvent. extract made of gel with components carbopol 940(0-100%) and glycerin(0-100%) with respond criteria physical properties such as pH, spreadability, sticking and protection power. The optimum formulated based on Simplex Lattice Design<em> </em>was the combination of carbopol 50 % : glycerol 50 %. Optimum results are obtained gel has a good physical properties. The test results showed that the effectiveness of antiseptic hand gels have generated a good antiseptic. Acceptability test results showed that the gel can be accepted by society.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

Physical Properties of Building Blocks from Hemp Shives Aggregate and Cementitious Binder, Manufactured in the Expanded Clay (Vibro Pressing) Production Line

2017 ◽  
Vol 908 ◽  
pp. 118-122 ◽  
Author(s):  
Giedrius Balčiūnas ◽  
Viktor Kizinievič ◽  
Justinas Gargasas
Keyword(s):  
Mechanical Properties ◽  
Thermal Conductivity ◽  
Compressive Strength ◽  
Physical Properties ◽  
Production Line ◽  
Scientific Literature ◽  
Building Blocks ◽  
Freeze Thaw ◽  
Cementitious Binder ◽  
Pressing Technology

Scientific literature mostly aims at investigation of composites with fibre hemp shives (FHS) aggregate and lime binder, although, such materials are characterised by pretty low mechanical properties. In order to obtain higher mechanical properties of a composite, it is appropriate to use cementitious binder. This work investigates physical properties of blocks from hemp shives aggregate and cementitious binder, manufactured in the expanded clay production line using vibro pressing technology. Following properties of the blocks are determined: freeze-thaw resistance, compressive strength, thermal conductivity and density. Thermal resistance according to EN ISO 6946 for the block with cavities is calculated as well. It is found that compressive strength of FHS-cement blocks may be up to 3.18 MPa when the density is of ~850 kg/m3 and thermal conductivity up to 0.135 W/(m∙K). It is found as well that the decrease of compressive strength is 8.7% after 25 freeze-thaw cycles.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

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