scholarly journals The Effect of Callyspongia Biru Antibacterial Extract Concentration Towards the growth of Bacterial Isolates of Eucheuma cottonii Seaweed with Ice-ice Disease

2021 ◽  
Vol 3 (2) ◽  
pp. 102
Author(s):  
Feliksitas Angel Masing
Keyword(s):  
Biology Education ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Randomized Design ◽  
Total Treatment ◽  
Completely Randomized Design ◽  
Difference Test ◽  
One Way Anova ◽  
Growth Of Bacteria ◽  
Eucheuma Cottonii

This research was conducted at the Biology Education Laboratory, Faculty of Teacher Training and Educational Sciences, Nusa Cendana University, with the aim of knowing the effect of the concentration of the antibacterial extract of Callyspongia biru on the growth of bacteria that caused Ice-ice disease. The method used in this study was an experimental method, with a Completely Randomized Design (CRD) consisting of 4 treatments (concentration of Callyspongia blue extract of 10%, 20%, 30% and 40%) with 3 times repetition. Therefore, the total treatment units were 12 units. The parameter that has been measured in this study was the diameter of the inhibition zone. The inhibition zone diameter data was then analyzed by using one-way ANOVA with the Tukay method.If Fcount  is greater than Ftable then it would be tested further using Honest Reality Difference test at 5% significant level. The result showed that the different concentrations of Callyspongia blue extract gave significantly different effects on the growth of bacterial isolates from seaweed Eucheuma cottonii with Ice-ice disease. At a concentration of 40%, Callyspongia biru extract showed the strongest average inhibition of 27.67 mm.

scholarly journals Pengaruh pH terhadap Produksi Antibakteri oleh Bakteri Asam Laktat dari Usus Itik

2017 ◽  
Vol 15 (3) ◽  
Author(s):  
Rudy Sutrisna ◽  
Christina Nugroho Ekowati ◽  
Edelina Sinaga Sinaga
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Analysis Of Variance ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Effect Of Ph ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to determine the effect of pH on the production of antibacterial by lactic acid bacteria. This study uses a completely randomized design factorial 3X 5 (3 X 5 treatment of bacterial isolates pH) with three replications. The first factor consisted of pH 4, pH 5, pH 6, pH 7, pH 8, and the second factor that isolates B2, B7, B8, whereas the parameters consists of a clear zone. The amount of production is determined by the size of the diameter of antibacterial inhibition zone against Salmonella sp. Differences in the production of antibacterial determined based on the results of analysis of variance. The treatment significantly further test BNT at 5% level. The results showed that the effect of media pH test based on the potential for the production of antibacterial effect. The diameter of the largest clear zone produced by B7 isolates at pH 6 was 17.2 mm, 17.1 mm B8 and B2 at pH 7 at 16.7 mm. The smallest diameter of the clear zone produced by B8 at pH 4 was 15.1 mm, B2 at pH 6 there is a clear zone of 12.7 mm and isolate B7 at pH 5 of 11.5 mm Keywords: Antibacterial, Lactic Acid Bacteria, pH, Salmonella sp, Clear zone

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

scholarly journals The Robusta coffee grounds residues to adsorb the heavy metal Lead (Pb) in the water

2018 ◽  
Author(s):  
Anita Dewi Moelyaningrum ◽  
Khoirul Ngibad ◽  
S.Pd Lilla Puji Lestari ◽  
Dessy Agustina Sari ◽  
Cahya Fajar Budi Hartanto ◽  
...  
Keyword(s):  
High Carbon ◽  
Control Groups ◽  
Robusta Coffee ◽  
Randomized Design ◽  
Coffee Grounds ◽  
Coffee Ground ◽  
Completely Randomized Design ◽  
Post Hoc ◽  
One Way Anova ◽  
Ks Test

This paper has been published in IOP Conf. Series: Journal of Physics: Conf. Ser1i2e3s415161748(9200‘1’8“)”012058 Lead (Pb) were toxic. Lead found in pipes, batteries, paint, dyes ceramic glaze, gasoline, and final waste disposal. The robusta coffee grouds residues had high carbon, nitrogen etc which can adsorb heavy metal.The purpose of this study is to analyze the robusta coffee grounds residues to adsorb the Pb in the water. The method of this research is a True Experimental using completely randomized design (CRD) method. There were control groups (C) and three treatments groups (T1; T2;T3; 5gram/ litre; 8gram/ litre; 10gram/ litre) with six repetitions. The robusta coffee ground residues were contacted for 2 hours.Total samples were24 samples which analyzed each parameter of the Pb with Atomic Adsorption Spectophotometry Analysis. The results showed that the more coffee ground residues that are exposed, increasingly turbid. The KS test showed that data were a normal distribution (sig=0,324). One way ANOVA test; Turkey post Hoc showed that there was sig difference between the control and treatment (F=4,326, Sig= 0,017). There were sig difference between control and treatment 2 and 3 (Sig=0,019; Sig=0,038). Robusta coffee grounds residues can reuse to adsorb the Pb pollution in the water. It can be a solution for treating the lead pollution in the water because of it easy to the application.

scholarly journals UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI
Keyword(s):  
Plant Height ◽  
Growth Media ◽  
Growing Media ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

scholarly journals Sensitivity Testing of Ciplukan (Physalis angulata) Rod Crude Extract on The Bacterium Pseudomonas fluorescens In Vitro

2019 ◽  
Vol 10 (1) ◽  
pp. 24-30
Author(s):  
Ion Tarsardo Sianturi ◽  
Arief Prajitno ◽  
Ellana Sanoesi
Keyword(s):  
Experimental Method ◽  
Crude Extract ◽  
Inhibition Zone ◽  
Natural Material ◽  
Sensitivity Testing ◽  
Randomized Design ◽  
Physalis Angulata ◽  
Completely Randomized Design

Diseases is a problem in aquaculture, one of which is the diseases caused by the bacteriumP.fluorescens. Antibiotics and chemicals materials themselves can give the bacterium resistance and cause harm for the environment. One alternative that can be done is using a natural material, namely the rod of ciplukan (p. angulata). The purpose of this research was to explain the effect of P. angulata on the growth of p. fluorescens. The method which can be use is experimental method by using the research design of completely randomized design (ral) with 5 treatment and 2 control with 3 repetitions. The results showed that extract of P. angulata at various dose (6,67 ppt, 13,33 ppt, 19,99 ppt, 26,66 ppt and 33,33 ppt) exhibited anti-P. fluorescens activity with inhibition zone diameters in the range of (4,06±0,07 mm - 9,63±1,61 mm). Increashing extract dose lead to increased the inhibition zone. The extract dose of 33,33 ppt exhibited best anti-P. fluorescens activity in this research. The research results show that P. angulata is proven to be able to inhibit the P. fluorescens, but to prove the effectiveness of this material, an in vivo is required.

scholarly journals PENGARUH SUBSTITUSI TEPUNG IKAN DENGAN TEPUNG KEPALA IKAN PATIN (Pangasius sp) TERHADAP PERTUMBUHAN IKAN LELE SANGKURIANG (Clarias sp)

2018 ◽  
Vol 6 (2) ◽  
pp. 129-140
Author(s):  
Yesica Manullang ◽  
Limin Santoso ◽  
Tarsim .
Keyword(s):  
Growth Rate ◽  
Fish Meal ◽  
Daily Growth Rate ◽  
Daily Growth ◽  
Protein Retention ◽  
Weight Growth ◽  
Randomized Design ◽  
Absolute Growth ◽  
Completely Randomized Design ◽  
Difference Test

ABSTRACTThe research was conducted at the Aquaculture Laboratory, University of Lampung in May to July,  2018. The aim of this study is to determine the effect of substitution of fish meal with catfish head flour on the growth of catfish, and head flour dosage the best for catfish growth. The study used a Completely Randomized Design with 3 treatments and 3 replications A (100% fish meal), B (70% fish meal and 30% catfish flour), and C (40% fish meal and 60% catfish flour). Parameters observed included absolute growth, daily growth rate, protein retention, feed convertion ratio (FCR), survival rate (SR), and supporting parameters that is feed cost and water quality. The data obtained were analyzed by variance and continued with smallest real difference test with a 95% confidence level. The results showed that the proportion of catfish head flour in artificial feed had an effect on absolute weight growth and daily growth rate (P, 0.001). The proportion of the use of 30% starch in the feed of catfish has a real influence on absolute growth (40.25 ± 5.67 g), and daily growth rate (0.67 ± 0.09 g/day).Keywords : Catfish, catfish head waste, substitution, feed, growth

scholarly journals PENGARUH JARAK TANAM PADA PENGGUNAAN METODE BUDIDAYA RAKIT TALI TERHADAP BERAT RUMPUT LAUT (Eucheuma cottonii) DI DUSUN TOISAPU KECAMATAN LEITIMUR SELATAN

2016 ◽  
Vol 2 (2) ◽  
pp. 106-111
Author(s):  
Etnah Garpenassy ◽  
H Tuaputty
Keyword(s):  
Growth Rate ◽  
Local Community ◽  
Randomized Design ◽  
Preparation Phase ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Planting Distance ◽  
Cultivation Techniques ◽  
Implementation Stage ◽  
Eucheuma Cottonii

Background: Toisapu village is one of village in Ambon Island which have potential to develop seaweed. Prospects Seaweed cultivation is very beneficial if growth and quality can be developed as a base for the production of various purposes in the industrial world. However, cultivation techniques undertaken by the local community have not fully had good planting quality. Method: The study includes the preparation phase, the implementation stage, and the observation stage. The parameters measured were the growth rate of Eucheuma cottonii seaweed is weight in the form of gram, using Completely Randomized Design (RAL) with 4 treatment levels and 6 replications. Results: The results of this study show that at a distance of 30 cm seaweed growth experienced a better growth rate when compared with other growth spacing, with wet weight for 45 days was 218 grams. Conclusion: Plant spacing affects the weight of seaweed, a good planting distance for seaweed growth is 30 cm at sea ebb and sea level with average wet weight for 45 days (6 weeks) is 218 grams.

scholarly journals UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC

2017 ◽  
Vol 5 (2) ◽  
pp. 47 ◽  
Author(s):  
Kadek Sudarmi ◽  
Ida Bagus Gede Darmayasa ◽  
I Ketut Muksin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Syzygium Cumini ◽  
E Coli ◽  
Randomized Design ◽  
Test Concentration ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Test Level

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins.   Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC  

scholarly journals Giving Lemna Minor and Mud to the Growth of Tubifex sp.

2019 ◽  
Vol 1 (3) ◽  
pp. 23-27
Author(s):  
Mahendra Mahendra ◽  
Doni Damara ◽  
Mursidatun Nufus ◽  
Vira Riana Putri
Keyword(s):  
Analysis Of Variance ◽  
Lemna Minor ◽  
Research Results ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Difference Test

This study aims to determine the effect of the use of a combination of lemna minor substrates and mud on the biomass of the population of Tubifex sp. This research is experimental, with a completely randomized design (CRD), with five levels of treatment P1 = 100% (lemna minor ) + 2,000 gr (mud), P2 = 75% (lemna minor ) + 2,000 gr (mud), P3 = 50 % (lemna minor ) + 2,000 gr (mud), P4 = 25% (lemna minor) + 2,000 gr (mud), P5 = 0% (lemna minor) + 2,000 gr (mud). Data obtained from research results in analysis of variance (ANOVA) and in further testing with the Smallest Significant Difference test. The main parameters observed were the number of population and biomass of Tubifex sp. The results of the study showed that the combination of Lemna minor substrate and mud significantly (p <0.05) on the growth of biomass and the population of Tubifex sp. The best results were found in the treatment with the treatment formulation P1 = 100% (lemna minor ) + 2,000 gr (mud) which produced a population of 6,250 ind / gr and biomass 8.60 gr / m2.

scholarly journals Tingkat kepadatan populasi Alona sp. pada media tumbuh ragi

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Henneke Pangkey ◽  
Sartje Lantu ◽  
Fanny Silooy
Keyword(s):  
Stocking Density ◽  
Quality Parameter ◽  
Water Quality Parameter ◽  
Well Water ◽  
Density Level ◽  
Horse Manure ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The One ◽  
One Way Anova

The purpose of this research was to investigate the population density of Alona sp. on yeast media with different concentrations. The study was conducted using a completely randomized design (CRD) consisting of 5 treatments and 3 replications for 21 days. The treatments given were A: 10 ppm yeast, B: 5 ppm yeast, C: 1 ppm yeast, D: horse manure, and E: well water. The initial stocking density of Alona sp. for each treatment was 15 individuals/300 mL of well water. The water quality parameter measured during the study was temperature. The results of the one-way ANOVA test showed that there were significant differences between treatments on the density level of Alona sp. (p <0.05). Tukey's further test showed that treatment B was the best with a density of Alona sp. as many as 8059 individuals/300 mL of well water. The temperature during the study, in the morning was 26 ± 0.52°C and in the afternoon was 26 ± 0.46°C.

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