ASSESSMENT OF INTRA- AND INTER- SPECIES OF THE FIRST GENERA-TION SOYBEAN HYBRIDS

Author(s):  
N. G. Kalitskaya ◽  
V. T. Sinegovskaya ◽  
T. P. Kobozeva

The article presents the results of the study of interspecific and intraspecific first generation soybean hybrids in terms of selection and genetic parameters. The studies were carried out on an experimental field of the breeding laboratory of the FSBSI FRC "All-Russian Scientific Research Institute of Soybean" in 2018 – 2019 in order to identify the best genotypes already in the first generation and predict an increase in the selection efficiency in subsequent generations. Hybridization was carried out according to the method of K.K. Malish and T.P. Ryazantseva by forced re-pollination. Highly productive varieties of the Institute breeding Kitrossa, Intriga, Nevesta and the Chinese variety Heihe 27 were used as maternal forms, samples from the collection of wild soybeans KBl-18, KZ-671, KBl-30, KM-705 and varieties Hedi and Tundra were used as paternal forms. The parental forms and the obtained hybrids were analyzed according to the traits: the number of productive nodes, beans, seeds per plant, weight of seeds from one plant and 1000 seeds. The interspecific hybridization analysis of soybeans showed that when crossing cultivars with wild forms, heterosis did not manifest itself in the first generation for any of the economically valuable traits. Hybridization of cultivated soybeans with the wild form is unpromising for the inheritance of economically valuable traits in order to obtain highly productive varieties. Productive hybrids were revealed during intraspecific hybridization of cultivated forms. In the hybrid combination ♀Heihe 27 x ♂Tundra, an increase in heterosis was noted in three traits: the number of beans (13.3%), the mass of seeds per plant, and the number of seeds (45%). The hybrids obtained by crossing of ♀Kitross x ♂Tundra had an advantage over the parental forms in the number of productive nodes and beans, the number of seeds per plant. The size of the seeds was inherited through the maternal line. The degree of phenotypic dominance was revealed only on one trait - the number of seeds per plant (5%). The heterosis effect was noted on the number of productive nodes (80%) and beans (83.3%).

2020 ◽  
Vol 17 ◽  
pp. 00084
Author(s):  
Olga Antoshina ◽  
Julia Odnodushnova ◽  
Gennadiy Fadkin ◽  
Irina Kondakova ◽  
Olga Fedosova

In the conditions of the south of the Nonchernozem Zone, intraspecific hybridization of winter soft wheat was carried out. When selecting parental forms used in crossing, special attention was paid to such economically valuable traits as productive tilling capacity, the number of grains and the mass of grain per spike. The true (Htru) and hypothetical heterosis (Hhyp) on the basis of “plant height”, “length of the lower internode”, “length of the upper internode”, “general tilling capacity”, “productive tilling capacity”, “spike length”, “number of spikelets”, “number of grains” and “grain weight per spike” were determined. It was established that the inheritance of quantitative traits in hybrids of the first generation was distinguished by the complex nature of distribution by types. Studies have made possible to identify 5 hybrid combinations F1, in which the effect of heterosis manifests itself simultaneously on five quantitative characteristics (ear length, number of spikelets per ear, number of grains per ear, grain weight per ear, mass grain from a plant).


Author(s):  
E.F. Myagkikh ◽  
◽  
I.N. Korotkikh ◽  

The purpose of the research is a comparative study of the Origanum vulgare L. varieties ‘Raduga’, ‘Zima’, ‘Slavnitsa’ bred in the FSBSI All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (VILAR) according to morphological and biological characteristics and economically valuable traits under conditions of the Foothill zone of the Crimea. The productivity of the aforementioned varieties amounted to 1.05±0.2–1.90±0.3 kg/m2; mass fraction of essential oil – 0.04±0.06–0.06±0.24 % of fresh weight and 0.10 ±0.55–0.17±0.61% of absolutely dry weight; essential oil collection – 0.4–0.9 g/m2. Germacrene D (18.4–19.5%) and β-caryophyllene (18.8–27.0%) are the main components of the essential oil collected from some varieties of Origanum vulgare L.


2021 ◽  
Vol 181 (4) ◽  
pp. 56-64
Author(s):  
M. R. Gonikova ◽  
V. I. Khoreva ◽  
V. G. Goldshtein ◽  
L. P. Nosovskaya ◽  
L. V. Adikaeva ◽  
...  

Background. Innovative technologies for deep processing of grain are widely used in maize grain processing with the release of the grain germ for subsequent extraction of oil and starch from it or production of sugar substitutes in the form of fructose syrup and alcohol, bakery or feed protein. A search for economically valuable sources of maize starch, useful byproducts of its processing, and natural modification of starch for deep processing is vital.Materials and methods. Ten high-starch maize accessions from the VIR collection, isolated by IR spectrometry, were studied. Starch content was measured according to GOST 10845-98, GOST 13586.5-93, GOST 10847-74 and GOST ISO 6647-1-2015 standards. Processing of grain into starch and assessment of the content of amylose and byproducts were carried out at the All-Russian Scientific Research Institute for Starch Products in 2018 according to L. P. Nosovskaya et al. The actual content of starch and its byproducts during deep grain processing was determined. The values of the actual percentage of dry matter (DM, %) in grain, mass fraction of starch, ash content, as well as the yield of extract, pulp, embryo, gluten, process water and starch were determined.Results. Accessions with a high yield of starch (k-4520, k-9301 and k-24730), germ (k-4520. k-8785 and k-24731), pulp (k-4520, k-8785, k-9991 and k-24732) and protein (k8785) were identified as well as those with a percentage of amylopectin in starch above 82% (k-24730 and k-24733) and 100% (k-5461 and k-9991), and amylose above 30% (k4520 and k-9301).Conclusion. Of practical interest in terms of the actual yield of starch (% DM in grain) are accessions k-4520, k-9301, k-24730, k-9991, k-5461 and k-4520. According to the results of breeding tests, accessions k-24730, k-24732 and k-24733 had the following values of starch harvest calculated for grain yield: 4.66, 4.41 and 4.18 t/ha, respectively. 


Knowledge of the nature of inheritance of traits of seed productivity in hybrid populations allows more efficient selection of highly heterozygous hybrids and rejection of low-value breeding material in the early stages of selection. Obtaining a qualitatively new source material of oilseed flax, created with the help of germplasm of wild species, requires genetic studies of traits associated with plant productivity, which are important in the selection of oilseed flax for high yields. Wild species of flax, such as L. angustifolium, L. hispanicum, L. bienne, L. crepitans are valuable genetic sources for obtaining disease-resistant forms, with early ripening, a large number of stems, side shoots, boxes on the plant. The aim of the research was to establish the features of inheritance of interspecific and intraspecific hybrids of the first generation of traits of seed productivity. The study was conducted at the Institute of Oilseeds NAAS in 2018-2019. The research material was interspecific and intraspecific hybrids of the first generation, obtained by crossing the full diallel scheme of two wild annual homostil species L. angustifolium and L. hispanicum and three samples of cultivated flax M 32/2 (IOC NAAS), L 5 (Czech Republic), L 6 (India), contrasting in terms of seed productivity (number of boxes per plant, number of seeds per plant, weight of 1000 seeds). Hybrids were studied in a hybrid nursery F1 according to the scheme: maternal form – direct and reverse hybrids F1 – paternal form. Structural analysis was performed on 10 plants of the parent components and on all F1 plants. The nature of the dominance of traits of seed productivity was determined by the degree of dominance (Beil, Atkins 1965). As a result of research it was established that the inheritance of the number of boxes on the plant in interspecific and intraspecific hybrids was dominated by positive overdominance (46,5% and 45,8%, respectively). In the inheritance of the number of seeds from the plant in interspecific hybrids, intermediate inheritance was predominant (44,7%); in intraspecific hybrids – positive supremacy (66,6%). On the basis of "weight of 1000 seeds" for interspecific and intraspecific hybrids, intermediate inheritance was predominant (95,9% and 62,5%, respectively). Our research allows us to conclude that the nature of inheritance of traits of seed productivity depends on a number of factors: genetic characteristics of experimental material, the studied trait, year conditions, type of crossing (interspecific or intraspecific). The obtained scientific data and genetic material have both theoretical and practical significance. The interspecific hybrid combinations M 32/2 / L. hispanicum, and L 6 / L. hispanicum and intraspecific M32/2 / L 6 and M32/2 / L 5 were isolated, in which heterosis was manifested by the number of boxes on the plant and the number of seeds from the plant. They will be used to create early-maturing varieties of oilseed flax with high seed productivity.


2018 ◽  
pp. 29-32
Author(s):  
O. R. Davletbaeva ◽  
M. G. Ibragimbekov ◽  
A. N. Khovrin

In commodity farms, the technology of growing onions is sown by sowing seeds from a one-year crop. This, in turn, introduces new requirements for growing technology, technical means and, most importantly, for varieties and hybrids of onions. The average yield of onion in Russia is at a low level - 20-30 tons / ha. New varieties and hybrids must have precocity, high yield, resistance to diseases and pests, good crabness. The success of selection work, as is known, is largely determined by the source material. The priority is to create heterotic hybrids of onion, showing increased viability, high stable yield and leveling, general resistance to pathogens, increasing the content of vitamins, proteins, dry substances, increasing cold resistance, drought resistance, and keeping quality. In the department of selection and seed production of All-Russian Scientific Research Institute of Vegetable Growing – a branch of FSBSI FSVC is breeding onions for growing in the annual culture of the Central strip of Russia. The study was conducted in 2017 with the setting of experiments in the open field. In the collection nursery 24 samples of onions of different geographical origin were investigated: Russia, the Netherlands, Italy and the USA. During the study, phenological, biometric measurements and a morphological description of the samples were carried out. As a result of the study of a set of economically valuable traits, the following samples of onion were identified and involved in the work: Ermak, Stuttgarter Riesen, Sturon, Forward, Crx 2381 F1, Santero F1, Sandra F1, Lamyca F1, Bennito F1, Premium F1.


2018 ◽  
Vol 12 (2) ◽  
pp. 46-52 ◽  
Author(s):  
A.I. Varlamova

The purpose of the research: study of the influence of increased doses of fenbendazole supramolecular complex (FSMC) on sheep’s organism. Materials and methods. The experiment was carried out at the Podolsk Department of All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin on 20 manorial invasion-free sheep aged 2-3 years old. Animals were divided according to the principle of analogues into 4 groups, 5 heads in each group. Animals of the 1, 2 and 3 group were orally administered with FSMC given as a single dose of 2, 6, 10 mg/kg, respectively, according to the active substance, i.e in therapeutic and in a dose increased by 3 and 5 times. Sheep of the fourth group didn’t receive the drug and they were as control. Study of clinical, hematological and biochemical parameters of animals from all groups was conducted 1 day before and in 1, 3, 5 days after administration of the drug by means of standard methods. Results and discussion. FSMC in therapeutic dose as well as in a dose increased by 3 and 5 times doesn’t have negative influence on clinical, hematological and biochemical parameters of the sheep. State of the sheep, which received the drug in doses of 20, 60, 100 mg/kg, was within the physiologically normal state and didn’t differ from the state before administration of the drug and from the animals of the control group. Drug security index exceeds 5. Red blood cell count, white blood cell count, hemoglobin count, leukogram parameters as well as biochemical parameters of blood: activity of alkaline phosphatase and amylase, bilirubin, creatinine, urea and glucose counts were within normal limits and didn’t differ from the parameters of the control animals.


Author(s):  
С. І. Силенко ◽  
О. С. Силенко

Доведено, що ступінь фенотипового домінування у гібридів F1 може варіювати від hp > +1 до hp < -1 залежно від компонентів схрещування. Встановлено, що переважна більшість гібридів F1 тривалість веґетаційного періоду та його міжфазних періодів успадковують за типами позитивного наддомінування та позитивним домінуванням. Успадкування висоти рослин відбувається за типами позитивного домінування, проміжного успадкування та за типом негативного домінування. Характер успадкування висоти прикріплення нижнього ярусу бобів у значній мірі визначається поєднанням батьківських компонентів. Так, у першому поколінні у гібридних комбінаціях спостерігається явище наддомінування. За ознаками урожайності та її елементів (урожайність насіння, маса насіння з рослини, маса 1000 насінин, кількість бобів на рослині, кількість насінин із рослини, кількість насінин у бобі) у всіх без виключення гібридів першого покоління спостерігався гетерозис. It was demonstrated that the degree of phenotypic predominance in F1 hybrids can vary from hp to hp < +1 > -1 depending on mating components. The vast majority of the F1 hybrids inherit the length of the growing season and its interphase by type of over& positive dominance. F1 hybrids inherit the height of plants by types of positive dominance, intermediate inheritance and negative domination. The height of the attachment of the lower tier of the beans is largely determined by a combination of parent components. Thus, in the first generation in hybrid combinations there is the phenomenon of over dominance. Due to the yield and its components (seed yield, seed mass per plant, 1000 seeds, number of beans per plant, number of seeds per plant, number of seeds per seedpod) in all first generation hybrids heterosis is observed.


2019 ◽  
Vol 180 (1) ◽  
pp. 59-65
Author(s):  
M. V. Gurkina

Background. The problem of a deficit in vegetable protein and the need to diversify agricultural produce require involvement of new and little-known crops, such as cowpea, in the production. Cowpea (Vigna unguiculata (L.) Walp.) is a high-protein leguminous crop; its breeding and production have been intensified in Russia only in recent years. The study of the VIR collection, carried out at the Institute’s branch in Astrakhan, makes it possible to disclose the crop’s breeding potential. Objective: Studying the range of variability and tracing the relationships of economically valuable traits in vegetable (green pod) cowpea under the conditions of Astrakhan Province.Materials and methods. In 2008‑2010, a study was implemented at an experiment plot of the Astrakhan branch of VIR. Thirty- three accessions of green pod cowpea from the VIR collection were studied to analyze the variability of 12 phenotypic traits. Positive and negative correlations were identified between the following characters: interphase periods (flowering, industrial ripeness and seed maturation), shape of the shrub, lengths of the stem and to the first pod, length and weight of the pod, number of seeds per pod, presence of a parchment layer and fiber in pod valves, pod yield per plant, number of pods and peduncles, seed weight per plant, and weight of 1000 seeds.Results and conclusion. Medium variability was observed for such characters as the interphase periods ‘shoots–industrial ripeness’ and ‘shoots– seed maturation’, number of seeds per pod, and weight of 1000 seeds: the coefficient of variation (CV) was 15‑18%. High variation was registered for the length and weight of the pod (CV = 26‑32%), shrub shape, stem length, and length of the stem up to the first pod (CV = 35‑37%). The widest ranges of variability were demonstrated in the quantitative yield characters of cowpea accessions: number of pods per plant (CV = 33‑49%), number of flower stalks (CV = 38‑51%), and seed weight per plant (CV = 38‑44%), which means that cowpea yield is quite susceptible to weather conditions. During the three years of studying, the lowest levels of variability in economically valuable traits were characteristic of the accessions of the early-ripening group: k‑668 and k‑873 (China); mid- ripening group: k‑971 (India); and late-ripening group: k‑141 (China). Cowpea pod productivity depended to the greatest degree from mean values of the number of pods per plant (r = 0,73) and the number of peduncles (r = 0,71). The identified correlations in the productivity components make it possible to optimize the selection of accessions promising as source materials for the development of new high-yielding cultivars.


2021 ◽  
Vol 36 ◽  
pp. 01019
Author(s):  
Dina Razantsvey ◽  
Evgenia Fokina ◽  
Valentina Sinegovskaya

The results of study of the samples of competitive variety testing in the Amur region are presented. The research was carried out in 2018-2020 on experimental plots of the breeding crop rotation of the All-Russian Scientific Research Institute of Soybean. As a result of the phytopathological survey, 12 best highly immune soybean samples were identified, of which the most adaptive (Ka > 1) to the conditions of the region were marked with 5 numbers: Amurskaya 24/42 (Ka = 1, 07), Amurskaya 24/54 (Ka = 1.07) and Amurskaya 24/60 (Ka = 1.11), Alena standard (Ka = 1.05), Amurskaya K-4/23 (Ka = 1.02) and Amurskaya K-14/17 (Ka = 1.05). As a result of a comprehensive assessment, considering the indicators of adaptability, productivity, and resistance to diseases, the 3 best soybean samples were identified: early-ripening – Amur 24/42; medium-ripening - Amur 24/54, Amur 24/60 with a yield of 2.78 (+ 0.46 t/ha to st); 2.80 (+ 0.32 t/ha to st); 2.75 (+0.27 t/ha to st). Two of which Am. 24/60 and Am. 24/42 were transferred in 2020 to the State Variety Testing for 2021-2022 as new highly productive, immune varieties Tisei and Luchistaya.


2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Ridwan Kusuma ◽  
Nyimas Sa’diyah ◽  
Yayuk Nurmiaty

Soybean consumption here in Indonesia continues to increase each year that is notaccompanied by an increase in soybean production. One way to increase soybeanproduction by using improved varieties. The purpose of this study were (1) Estimating thevalue of the diversity of phenotypes soy F6 generations from crosses between Wilis x Mlg2521,(2) Estimating the heritability estimates soybean F6 generations from crosses Wilis x Mlg2521,(3) Know the numbers expectation of F6 generation crosses Wilis x Mlg2521. The researchwas conducted from March 2014 until June 2014 at the Land Lab Lampung StatePolytechnic and observations made in Laboratory of Seed and Plant Breeding, University ofLampung. Soybean seeds used were F6 generation zuriat from Wilis x Mlg2521, Wilis andMlg2521. The treatment laid out in a randomized block design, 2 replications. The resultsshowed that (1) The diversity of phenotypes population F6 for characters date of flowering,harvesting age, plant height, number of productive branches, total number of pods plant,weight of 100 grains, grain weight plant and number of seeds plant including all the broadcriteria, (2) The value of heritability estimates the population F6 for characters date offlowering, harvesting age, plant height, number of productive branches, total number ofpods plant, weight of 100 grains, grain weight per plant and number of seeds plantbelonging to the high criteria, (3) Number expectations of the population F6 is 7-64-1-8 and7-64-1-3.Keywords: heritability, phenotypic variance, soybean


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